Mutualistic Bacteria Research Articles

A cellular automata-based model of rhizosphere colonization by mutualistic bacteria accounts for the role of quorum sensing on successful concentration near plant roots

A cellular automata-based model of rhizosphere colonization by mutualistic bacteria accounts for the role of quorum sensing on successful concentration near plant roots

Silkworm Gut Microflora as a Source: Biotechnological Applications

The term "microflora" has gained popularity as researchers explore the symbiotic relationships between microorganisms and their hosts, from our skin to our gut. Insects, including the silkworm, have mutualistic ties with their gut microflora, making them a valuable resource for studying essential molecules. The silkworm, with its rapid generation time, abundant genetic resources, well-defined genetic background, and numerous homologous genes, is an ideal model organism for various life science studies. Silkworm rearing is simple, inexpensive, and does not require ethical clearance, allowing the gut microflora of Bombyx mori to be harnessed for biotechnological applications. Silkworm gut microbes can synthesize and produce extracellular enzymes, such as cellulases, proteases, and lipases, which are crucial for industries ranging from biofuel production to waste management and textile processing. Additionally, these microbes can produce vitamins, such as vitamin B12, and metabolites like short-chain fatty acids (SCFAs), which have potential applications in the food and pharmaceutical industries. Antimicrobial substances produced by the gut microflora, including bacteriocins and lactic acid, are of particular therapeutic importance, offering natural alternatives to traditional antibiotics and contributing to the development of novel antimicrobial therapies. Moreover, the antioxidants generated by these symbionts, such as glutathione and superoxide dismutase (SOD), have significant implications for health supplements and cosmetics, owing to their ability to combat oxidative stress and support immune function. Beyond their metabolic capacities, silkworm symbionts can be used to enhance health, target and manage agricultural pests, and control vectors of human diseases in an environmentally friendly manner. Recent research has also highlighted the potential of mutualistic symbiotic bacteria for plastic disposal, with enzymes like PETases showing promise in degrading synthetic polymers. Despite extensive knowledge on the biology and physiology of the silkworm Bombyx mori, few studies have focused on its gut microflora. Modern genetic techniques, such as the genetic transformation of silkworms with genes of interest, may help overcome these research gaps. Increasing research efforts on silkworm gut microflora will not only reveal new symbiotic relationships but also identify new sources of biotechnologically important molecules and enzymes for therapeutic and industrial applications.

Social environment influences microbiota and potentially pathogenic bacterial communities on the skin of developing birds

BackgroundAnimal bacterial symbionts are established early in life, either through vertical transmission and/or by horizontal transmission from both the physical and the social environment, such as direct contact with con- or heterospecifics. The social environment particularly can influence the acquisition of both mutualistic and pathogenic bacteria, with consequences for the stability of symbiotic communities. However, segregating the effects of the shared physical environment from those of the social interactions is challenging, limiting our current knowledge on the role of the social environment in structuring bacterial communities in wild animals. Here, we take advantage of the avian brood-parasite system of Eurasian magpies (Pica pica) and great spotted cuckoos (Clamator glandarius) to explore how the interspecific social environment (magpie nestlings developing with or without heterospecifics) affects bacterial communities on uropygial gland skin.ResultsWe demonstrated interspecific differences in bacterial community compositions in members of the two species when growing up in monospecific nests. However, the bacterial community of magpies in heterospecific nests was richer, more diverse, and more similar to their cuckoo nest-mates than when growing up in monospecific nests. These patterns were alike for the subset of microbes that could be considered core, but when looking at the subset of potentially pathogenic bacterial genera, cuckoo presence reduced the relative abundance of potentially pathogenic bacterial genera on magpies.ConclusionsOur findings highlight the role of social interactions in shaping the assembly of the avian skin bacterial communities during the nestling period, as exemplified in a brood parasite—host system.

Activation of denitrification potential within anammox consortia to exceed nitrogen removal thresholds via Fe-Mn functionalized biochar: Enhanced interspecies electron transfer and metabolite cross-feeding

Anammox exerts a crucial role in nitrogenous effluents remediation; nevertheless, nitrogen removal efficiency thresholds triggered by additional nitrate production poses a critical obstacle for anammox performance. Herein, Fe-Mn functionalized biochar (FeMn#BC) was synthesized and incorporated into anammox consortia to elicit the exogenous organics-independent metabolic potential of denitrifying bacteria, which decreased nitrate accumulation and achieved up to 90.69 ± 0.48 % total nitrogen removal efficiency. Enzyme content analysis indicated that FeMn#BC bolstered biometabolic vigour and electron transport system activity by stimulating the synthesis of heme c and NADH. Microbial community succession results demonstrated that FeMn#BC facilitated the proliferation of anammox bacteria (AnAOB) and nitrate-reducing functional bacteria. Metagenomic analysis uncovered that FeMn#BC increased the relative abundance of genes related to energy metabolism, nutrient-substrate ingestion, cofactor synthesis, and iron-manganese transport pathways. Candidatus Brocadia sp013360945 interacted nitrogen metabolism substrates and electrons with dominated denitrifying species PR03 sp. (dependent on endogenous organics and FeMn#BC-released Fe(II)), while interacted cofactors with other mutualistic bacteria possessing nitrate-reducing potential (e.g. ATM1 sp003577165). FeMn#BC-released Mn(II) served as an additional electron donor, augmenting electron availability for non-dominant anammox species like Candidatus Jettenia caeni and Candidatus. Kuenenia stuttgartiensis. This research highlights the critical mechanism of Fe-Mn synergism for overcoming the bottleneck of anammox nitrogen removal efficiency, and demonstrates the substantial potential of Fe-Mn functionalized materials to revolutionize the existing wastewater treatment technologies towards a carbon-free approach.

Does introduced European Phragmites australis experience belowground microbial enemy release in North America?

AbstractEscape from native range enemies can give invasive species a competitive edge according to the enemy‐release hypothesis. While more commonly associated with predators and herbivores, release from belowground microbial antagonists has been recently demonstrated to benefit invasive plants. Biogeographic variation in dominance and comparisons of soil communities suggest that invasive European Phragmites australis may have also benefitted from belowground enemy release in North America (NA). Here we examine the effects of native range (Europe) versus introduced range (NA) soil communities on European and North American P. australis using a reciprocal inoculation seedling growth experiment. Contrary to the enemy‐release hypothesis, we found that North American P. australis was sensitive to soil community origin in that the seedlings grown in European soil communities had higher total biomass than seedlings grown in North American soil communities. This pattern was not observed in the European P. australis seedlings which had similar biomass when grown with North American or European soil communities. Notably, North American P. australis had higher biomass than European P. australis regardless of which soil community it was grown in, suggesting a growth–defense tradeoff. Though the relative abundance of mutualists and pathogens composition did not differ between the two ranges, an indicator analysis revealed that mutualistic fungi and bacteria were key components of European soil communities but not in North American communities. Interestingly, North American soil communities had lower β diversity than European communities suggesting higher levels of community conservation among North American populations. This research represents the first evidence of growth–defense trade‐offs in North American P. australis and offers a novel mechanism in understanding the invasion of P. australis in NA.

Reverse Vaccinology Approach to Identify Novel and Immunogenic Targets against Streptococcus gordonii.

Streptococcus gordonii is a gram-positive, mutualistic bacterium found in the human body. It is found in the oral cavity, upper respiratory tract, and intestines, and presents a serious clinical problem because it can lead to opportunistic infections in individuals with weakened immune systems. Streptococci are the most prevalent inhabitants of oral microbial communities, and are typical oral commensals found in the human oral cavity. These streptococci, along with many other oral microbes, produce multispecies biofilms that can attach to salivary pellicle components and other oral bacteria via adhesin proteins expressed on the cell surface. Antibiotics are effective against this bacterium, but resistance against antibodies is increasing. Therefore, a more effective treatment is needed. Vaccines offer a promising method for preventing this issue. This study generated a multi-epitope vaccine against Streptococcus gordonii by targeting the completely sequenced proteomes of five strains. The vaccine targets are identified using a pangenome and subtractive proteomic approach. In the present study, 13 complete strains out of 91 strains of S. gordonii are selected. The pangenomics results revealed that out of 2835 pan genes, 1225 are core genes. Out of these 1225 core genes, 643 identified as non-homologous proteins by subtractive proteomics. A total of 20 essential proteins are predicted from non-homologous proteins. Among these 20 essential proteins, only five are identified as surface proteins. The vaccine construct is designed based on selected B- and T-cell epitopes of the antigenic proteins with the help of linkers and adjuvants. The designed vaccine is docked against TLR2. The expression of the protein is determined using in silico gene cloning. Findings concluded that Vaccine I with adjuvant shows higher interactions with TLR2, suggesting that the vaccine has the ability to induce a humoral and cell-mediated response to treat and prevent infection; this makes it promising as a vaccine against infectious diseases caused by S. gordonii. Furthermore, validation of the vaccine construct is required by in vitro and in vivo trials to check its actual potency and safety for use to prevent infectious diseases caused by S. gordonii.

Shedding light on bacteria-host interactions with the aid of TnSeq approaches.

Bacteria are highly adaptable and grow in diverse niches, where they often interact with eukaryotic organisms. These interactions with different hosts span the entire spectrum from symbiosis to pathogenicity and thus determine the lifestyle of the bacterium. Knowledge of the genetic determinants involved in animal and plant host colonization by pathogenic and mutualistic bacteria is not only crucial to discover new drug targets for disease management but also for developing novel biostimulant strategies. In the last decades, significant progress in genome-wide high-throughput technologies such as transposon insertion sequencing has led to the identification of pathways that enable efficient host colonization. However, the extent to which similar genes play a role in this process in different bacteria is yet unclear. This review highlights the commonalities and specificities of bacterial determinants important for bacteria-host interaction.

Inorganic carbon limitation decreases ammonium removal and N2O production in the algae-nitrifying bacteria symbiosis system

Ammonium removal by a symbiosis system of algae (Chlorella vulgaris) and nitrifying bacteria was evaluated in a long-term photo-sequencing batch reactor under varying influent inorganic carbon (IC) concentrations (15, 10, 5 and 2.5 mmol L−1) and different nitrogen loading rate (NLR) conditions (270 and 540 mg-N L−1 d−1). The IC/N ratios provided were 2.33, 1.56, 0.78 and 0.39, respectively, for an influent NH4+-N concentration of 90 mg-N L−1 (6.43 mmol L−1). The results confirmed that both ammonium removal and N2O production were positively related with IC concentration. Satisfactory ammonium removal efficiencies (>98 %) and rates (29–34 mg-N gVSS−1 h−1) were achieved regardless of NLR levels under sufficient IC of 10 and 15 mmol L−1, while insufficient IC at 2.5 mmol L−1 led to the lowest ammonium removal rates of 0 mg-N gVSS−1 h−1. The ammonia oxidation process by ammonia oxidizing bacteria (AOB) played a predominant role over the algae assimilation process in ammonium removal. Long-time IC deficiency also resulted in the decrease in biomass and pigments of algae and nitrifying bacteria. IC limitation led to the decreasing N2O production, probably due to its negative effect on ammonia oxidation by AOB. The optimal IC concentration was determined to be 10 mmol L−1 (i.e., IC/N of 1.56, alkalinity of 500 mg CaCO3 L−1) in the algae-bacteria symbiosis reactor, corresponding to higher ammonia oxidation rate of ∼41 mg-N gVSS−1 h−1 and lower N2O emission factor of 0.13 %. This suggests regulating IC concentrations to achieve high ammonium removal and low carbon emission simultaneously in the algae-bacteria symbiosis wastewater treatment process.

A mutualistic bacterium rescues a green alga from an antagonist

Photosynthetic protists, known as microalgae, are key contributors to primary production on Earth. Since early in evolution, they coexist with bacteria in nature, and their mode of interaction shapes ecosystems. We have recently shown that the bacterium Pseudomonas protegens acts algicidal on the microalga Chlamydomonas reinhardtii. It secretes a cyclic lipopeptide and a polyyne that deflagellate, blind, and lyse the algae [P. Aiyar et al., Nat. Commun. 8, 1756 (2017) and V. Hotter et al., Proc. Natl. Acad. Sci. U.S.A. 118, e2107695118 (2021)]. Here, we report about the bacterium Mycetocola lacteus, which establishes a mutualistic relationship with C. reinhardtii and acts as a helper. While M. lacteus enhances algal growth, it receives methionine as needed organic sulfur and the vitamins B1, B3, and B5 from the algae. In tripartite cultures with the alga and the antagonistic bacterium P. protegens, M. lacteus aids the algae in surviving the bacterial attack. By combining synthetic natural product chemistry with high-resolution mass spectrometry and an algal Ca2+ reporter line, we found that M. lacteus rescues the alga from the antagonistic bacterium by cleaving the ester bond of the cyclic lipopeptide involved. The resulting linearized seco acid does not trigger a cytosolic Ca2+ homeostasis imbalance that leads to algal deflagellation. Thus, the algae remain motile, can swim away from the antagonistic bacteria and survive the attack. All three involved genera cooccur in nature. Remarkably, related species of Pseudomonas and Mycetocola also act antagonistically against C. reinhardtii or as helper bacteria in tripartite cultures.

Comparative assessment of oestradiol removal in Scenedesmus-Rhodococcus and Microcystis-Raoultella symbiotic systems versus monoculture systems

ABSTRACT In this study, the abilities two kinds of algae, Scenedesmus and Microcystis aeruginosa, and two kinds of bacteria, Rhodococcus and Raoultella planticola, to remove oestradiol when in a symbiotic system were investigated. The effects of the combined systems as well as the effects of each bacterium and algae alone were examined. We then compared the improvements from the bacteria-algae symbiosis systems with Microcystis aeruginosa and Raoultella planticola. The results showed that the oestradiol removal rate of the best system was 97.6%, while that from the Microcystis aeruginosa and Raoultella planticola system was 89%. HIGHLIGHTS Oestradiol has an effect on algal growth. The effects of algae on the growth of degrading bacteria were investigated. Degrading bacteria can affect the growth of algae. The symbiosis of bacteria and algae effectively removed oestradiol, with a removal rate that reached 97.6%.

Entomopathogenic pseudomonads can share an insect host with entomopathogenic nematodes and their mutualistic bacteria.

A promising strategy to overcome limitations in biological control of insect pests is the combined application of entomopathogenic pseudomonads (EPPs) and nematodes (EPNs) associated with mutualistic bacteria (NABs). Yet, little is known about interspecies interactions such as competition, coexistence, or even cooperation between these entomopathogens when they infect the same insect host. We investigated the dynamics of bacteria-bacteria interactions between the EPP Pseudomonas protegens CHA0 and the NAB Xenorhabdus bovienii SM5 isolated from the EPN Steinernema feltiae RS5. Bacterial populations were assessed over time in experimental systems of increasing complexity. In vitro, SM5 was outcompeted when CHA0 reached a certain cell density, resulting in the collapse of the SM5 population. In contrast, both bacteria were able to coexist upon haemolymph-injection into Galleria mellonella larvae, as found for three further EPP-NAB combinations. Finally, both bacteria were administered by natural infection routes i.e. orally for CHA0 and nematode-vectored for SM5 resulting in the addition of RS5 to the system. This did not alter bacterial coexistence nor did the presence of the EPP affect nematode reproductive success or progeny virulence. CHA0 benefited from RS5, probably by exploiting access routes formed by the nematodes penetrating the larval gut epithelium. Our results indicate that EPPs are able to share an insect host with EPNs and their mutualistic bacteria without major negative effects on the reproduction of any of the three entomopathogens or the fitness of the nematodes. This suggests that their combination is a promising strategy for biological insect pest control.

Unraveling Microbial Endosymbiosis Dynamics in Plant-Parasitic Nematodes with a Genome Skimming Strategy

Bacterial endosymbionts, in genera Wolbachia and Cardinium, infect various arthropods and some nematode groups. Manipulating these microbial symbionts presents a promising biocontrol strategy for managing disease-causing parasites. However, the diversity of Wolbachia and Cardinium in nematodes remains unclear. This study employed a genome skimming strategy to uncover their occurrence in plant-parasitic nematodes, analyzing 52 populations of 12 species. A metagenome analysis revealed varying endosymbiont genome content, leading to the categorization of strong, weak, and no evidence for endosymbiont genomes. Strong evidence for Wolbachia was found in five populations, and for Cardinium in one population, suggesting a limited occurrence. Strong Wolbachia evidence was noted in Pratylenchus penetrans and Radopholus similis from North/South America and Africa. Heterodera glycines from North America showed strong Cardinium evidence. Weak genomic evidence for Wolbachia was observed in Globodera pallida, Meloidogyne incognita, Rotylenchus reniformis, Pratylechus coffeae, Pratylenchus neglectus, and Pratylenchus thornei; for Cardinium was found in G. pallida, R. reniformis and P. neglectus; 27/52 populations exhibited no endosymbiont evidence. Wolbachia and Cardinium presence varied within nematode species, suggesting non-obligate mutualism. Wolbachia and Cardinium genomes differed among nematode species, indicating potential species-specific functionality. This study advances knowledge of plant-parasitic nematode–bacteria symbiosis, providing insights for downstream eco-friendly biocontrol strategies.

Algal-bacterial consortium promotes carbon sink formation in saline environment

IntroductionThe level of atmospheric CO2 has continuously been increasing and the resulting greenhouse effects are receiving attention globally. Carbon removal from the atmosphere occurs naturally in various ecosystems. Among them, saline environments contribute significantly to the global carbon cycle. Carbonate deposits in the sediments of salt lakes are omnipresent, and the biological effects, especially driven by halophilic microalgae and bacteria, on carbonate formation remain to be elucidated. ObjectivesThe present study aims to characterize the carbonates formed in saline environments and demonstrate the mechanisms underlying biological-driven CO2 removal via microalgal-bacterial consortium. MethodsThe carbonates naturally formed in saline environments were collected and analyzed. Two saline representative organisms, the photosynthetic microalga Dunaliella salina and its mutualistic halophilic bacteria Nesterenkonia sp. were isolated from the inhabiting saline environment and co-cultivated to study their biological effects on carbonates precipitation and isotopic composition. During this process, electrochemical parameters and Ca2+ flux, and expression of genes related to CaCO3 formation were analyzed. Genome sequencing and metagenomic analysis were conducted to provide molecular evidence. ResultsThe results showed that natural saline sediments are enriched with CaCO3 and enrichment of genes related to photosynthesis and ureolysis. The co-cultivation stimulated 54.54% increase in CaCO3 precipitation and significantly promoted the absorption of external CO2 by 49.63%. A pH gradient was formed between the bacteria and algae culture, creating 150.22 mV of electronic potential, which might promote Ca2+ movement toward D. salina cells. Based on the results of lab-scale induction and 13C analysis, a theoretical calculation indicates a non-negligible amount of 0.16 and 2.3 Tg C/year carbon sequestration in China and global saline lakes, respectively. ConclusionThe combined effects of these two typical representative species have contributed to the carbon sequestration in saline environments, by promoting Ca2+ influx and increase of pH via microalgal and bacterial metabolic processes.

Comparative phylogenomics and phylotranscriptomics provide insights into the genetic complexity of nitrogen-fixing root-nodule symbiosis

Plant root-nodule symbiosis (RNS) with mutualistic nitrogen-fixing bacteria is restricted to a single clade of angiosperms, the Nitrogen-Fixing Nodulation Clade (NFNC), and is best understood in the legume family. Nodulating species share many commonalities, explained either by divergence from a common ancestor over 100 million years ago or by convergence following independent origins over that same time period. Regardless, comparative analyses of diverse nodulation syndromes can provide insights into constraints on nodulation—what must be acquired or cannot be lost for a functional symbiosis—and the latitude for variation in the symbiosis. However, much remains to be learned about nodulation, especially outside of legumes. Here, we employed a large-scale phylogenomic analysis across 88 species, complemented by 151 RNA-seq libraries, to elucidate the evolution of RNS. Our phylogenomic analyses further emphasize the uniqueness of the transcription factor NIN as a master regulator of nodulation and identify key mutations that affect its function across the NFNC. Comparative transcriptomic assessment revealed nodule-specific upregulated genes across diverse nodulating plants, while also identifying nodule-specific and nitrogen-response genes. Approximately 70% of symbiosis-related genes are highly conserved in the four representative species, whereas defense-related and host-range restriction genes tend to be lineage specific. Our study also identified over 900 000 conserved non-coding elements (CNEs), over 300 000 of which are unique to sampled NFNC species. NFNC-specific CNEs are enriched with the active H3K9ac mark and are correlated with accessible chromatin regions, thus representing a pool of candidate regulatory elements for genes involved in RNS. Collectively, our results provide novel insights into the evolution of nodulation and lay a foundation for engineering of RNS traits in agriculturally important crops.

Three cuticular amides in the tripartite symbiosis of leafcutter ants.

Cuticular hydrocarbons (CHCs) play various roles in insects' chemical ecology. As leafcutter ants live in a specific symbiosis with fungi, they harvest and with different bacteria, some of these CHCs might be associated with a mutualistic function within this symbiosis. To obtain a more precise picture in that respect we compared the CHC profiles of the leafcutter ants, Atta sexdens, Atta cephalotes, and Acromyrmex octospinosus inhabited by mutualistic bacteria with the profiles of Polyrhachis dives and Messor aciculatus by GC-EI-MS analysis and 28 other ant species by data from the literature. We were able to identify three alkyl amides (hexadecanamide, hexadecenamide, and tetradecanamide), occurring only in the CHC profiles of leafcutter ants inhabited by symbiotic bacteria. Our results lead to the conclusion that those alkyl amides could have a function in the tripartite symbiosis of leafcutter ants.

Optimizing Entomopathogenic Nematode Genetics and Applications for the Integrated Management of Horticultural Pests

Entomopathogenic nematodes (EPNs) can kill and recycle in their host populations, which bodes well for EPNs’ exploitation in long-term and safe pest management. However, EPNs’ cost and efficacy need transformational technology to supplant less expensive and more effective but toxic/unhealthy pesticides. A technology that allows for the significant uptake of commercial EPNs should both boost their market suitability and provide genetic improvements. This review provides brief overviews of EPNs’ biology and ecology from the standpoint of pest/pathogen management as a prerequisite for EPN improvements. Understanding the biology and ecology of EPNs, particularly their symbiotic relationships with bacteria, is crucial to their effective use in pest management. This review provides relevant insights into EPN-symbiotic bacteria and the EPN–symbiont complex. The symbiotic relationship between EPNs and bacteria plays a key role in IPM, providing unique advantages. Either of them can be included in mechanisms underlying the various positive sides of plant–insect interactions in emerging integrated pest management (IPM) systems. Recent approaches, in which EPNs can act additively or synergistically with other production inputs in IPM programs, are discussed for further expansion. The simultaneous favorable effects of EPNs and/or their mutualistic bacteria on several pest/pathogen species of crops should be identified. Merits, such as the rapid killing of insect pests, ease of EPN/the symbiont’s mass production and a broad host range, are presented in order to widely disseminate the conditions under which EPN usage can offer a cost-effective and/or value-added technique for IPM. To maximize the effectiveness of EPNs in IPM, various genetic improvement techniques are being explored. Such techniques, along with their merits/demerits and related tools, are reviewed to optimize the common biocontrol usage of EPNs. Examples of genetic improvements to EPNs that allow for their use in transformational technology, such as a cost-effective application technique, increased infectivity, and toleration of unfavorable settings, are given. Proper production practices and genetic techniques should be applied carefully to avoid undesirable results; it is suggested that these are considered on a case-by-case basis. This will enable us to optimize EPN performance based on the given variables.

Influence of nitrogen sources on wastewater treatment performance by filamentous algae in constructed wetland system

Although filamentous algae have the characteristics of high nutrient assimilation ability, and adaptation to different conditions, studies on their role in water purification of constructed wetlands (CWs) are limited. In this study, the wastewater treatment capacity under different nitrogen sources was explored by constructing a filamentous algal CW (FACW) system. Results confirmed the fast and stable operation efficiency of the FACW system. Ammonia nitrogen was preferred in Cladophora sp. absorption and assimilation. The nutrient consumption rate (NCR) for total nitrogen (TN) of AG was 2.65 mg g−1 d−1, much higher than that of nitrate nitrogen (NG) (0.89 mg g−1 d−1). The symbiosis of bacteria and Cladophora sp. Contributed to pollutant removal. A stable and diverse community of microorganisms was found on Cladophora sp. Surface, which revealed different phylogenetic relationships and functional bacterial proportions with those attached on sediment surface. In addition, temperature and light intensity have great influence on the purification ability of plants, and low hydraulic retention time is beneficial to the cost-effective operation of the system. This study provides a method to expand the utilization of wetland plants and apply large filamentous algae to the purification of wetland water quality.

Evaluation of Legume-Rhizobial Symbiotic Interactions Beyond Nitrogen Fixation That Help the Host Survival and Diversification in Hostile Environments.

Plants often experience unfavorable conditions during their life cycle that impact their growth and sometimes their survival. A temporary phase of such stress, which can result from heavy metals, drought, salinity, or extremes of temperature or pH, can cause mild to enormous damage to the plant depending on its duration and intensity. Besides environmental stress, plants are the target of many microbial pathogens, causing diseases of varying severity. In plants that harbor mutualistic bacteria, stress can affect the symbiotic interaction and its outcome. To achieve the full potential of a symbiotic relationship between the host and rhizobia, it is important that the host plant maintains good growth characteristics and stay healthy under challenging environmental conditions. The host plant cannot provide good accommodation for the symbiont if it is infested with diseases and prone to other predators. Because the bacterium relies on metabolites for survival and multiplication, it is in its best interests to keep the host plant as stress-free as possible and to keep the supply stable. Although plants have developed many mitigation strategies to cope with stress, the symbiotic bacterium has developed the capability to augment the plant's defense mechanisms against environmental stress. They also provide the host with protection against certain diseases. The protective features of rhizobial-host interaction along with nitrogen fixation appear to have played a significant role in legume diversification. When considering a legume-rhizobial symbiosis, extra benefits to the host are sometimes overlooked in favor of the symbionts' nitrogen fixation efficiency. This review examines all of those additional considerations of a symbiotic interaction that enable the host to withstand a wide range of stresses, enabling plant survival under hostile regimes. In addition, the review focuses on the rhizosphere microbiome, which has emerged as a strong pillar of evolutionary reserve to equip the symbiotic interaction in the interests of both the rhizobia and host. The evaluation would draw the researchers' attention to the symbiotic relationship as being advantageous to the host plant as a whole and the role it plays in the plant's adaptation to unfavorable environmental conditions.

Drosophila melanogaster Imd signaling interacts with insulin signaling and alters feeding rate upon parasitic nematode infection

Significant progress has been made in recent years on exploring immunometabolism, a field that integrates two processes essential for maintaining tissue and organismal homeostasis, immunity and metabolism. The nematode parasite Heterorhabditis gerrardi, its mutualistic bacteria Photorhabdus asymbiotica, and the fruit fly Drosophila melanogaster constitute a unique system to investigate the molecular basis of host immunometabolic response to nematode-bacterial complexes. In this study, we explored the contribution of the two major immune signaling pathways, Toll and Imd, to sugar metabolism in D. melanogaster larvae during infection with H. gerrardi nematodes. We infected Toll or Imd signaling loss-of-function mutant larvae with H. gerrardi nematodes and assessed larval survival ability, feeding rate, and sugar metabolism. We found no significant differences in the survival ability or levels of sugar metabolites in any of the mutant larvae when responding to H. gerrardi infection. However, we found that the Imd mutant larvae have higher feeding rate than controls during the early stages of infection. In addition, feeding rates are lower in Imd mutants relative to the control larvae as the infection progresses. We further showed that Dilp2 and Dilp3 gene expression increases in Imd mutants compared to controls early in the infection, but their expression levels decrease at later times. These findings indicate that Imd signaling activity regulates the feeding rate and Dilp2 and Dilp3 expression in D. melanogaster larvae infected with H. gerrardi. Results from this study facilitate our understanding of the link between host innate immunity and sugar metabolism in the context of infectious diseases caused by parasitic nematodes.

Responses of biofilm communities in a hybrid moving bed biofilm reactor-membrane bioreactor system to sulfadiazine antibiotic exposure

Antibiotics in wastewater can affect the structures and functions of bacterial communities, subsequently influencing how well a biological process performs. Therefore, the characteristics of bacterial community were investigated in a hybrid moving bed biofilm reactor-membrane bioreactor system when treating domestic wastewater containing sulfadiazine (SDZ). Results indicated total nitrogen removal reduced by 10.2%, 9.1%, 2.7% and 2.9%, respectively, with increasing carbon to nitrogen (C/N) ratios (2.5, 4, 6 and 9) when SDZ was present (0.5 mg/L). The microbial communities' analysis revealed that the abundance of nitrogen removal-related bacteria increased with C/N. Specifically, the abundance of ammonia-oxidizing bacteria (0.46%-0.90%) was low, and the nitrite-oxidizing bacteria (2.16%-7.13%) and denitrifying bacteria showed a significant increase (Hyphomicrobium: 0.57%-3.54%) when C/N ratio increased. The abundance of denitrifying bacterial declined by 4.82–8.56% at different C/N ratios, while nitrifying bacterial rose by 0.70–5.67%. Interestingly, the denitrifying bacteria Enterobacter, Sphingomonas and Gemmatimonas acted as mutualistic bacteria that stabilized denitrification.