Sweet Orange Peel Research Articles

Influence of the Rootstock and the Ploidy Level of the Scion and the Rootstock on Sweet Orange (Citrus sinensis) Peel Essential Oil Yield, Composition and Aromatic Properties

Rootstock is widely used for the cultivation of citrus fruits because it brings resistance or tolerance to diseases or environmental constrains and modulates the fruit quality. Polyploidization is a widespread improvement strategy in citrus. The objective was to evaluate the effect of rootstock and ploidy level on the composition of essential oils. Two trials were conducted, one displaying a ‘Navelina’ orange grafted on three rootstocks and a second combining two ploidy levels (di and tetraploid) of scion (‘Pineapple’ orange) and rootstock (‘Carrizo’ citrange). The composition of peel essential oil (PEO) was analyzed by gas chromatography coupled with mass spectrometry, and a panel of experts analyzed its flavor variation with a triangle test approach. The rootstock influenced the yield and composition of the orange PEO, with a low impact on flavor. Neither the rootstock nor the scion ploidy level affected the PEO yield. Only the tetraploid level of the scion significantly modified the PEO composition, reducing the oxygenated compound fraction. Sensitive significant differences were detected between the reference sample (diploid scion–diploid rootstock) and the three other combinations. These results suggest that for the profiling of an aromatic flavor, the rootstock is a key element as is the ploidy level of the scion.

Sweet Orange (Citrus sinensis) Peel Powder with Xylanase Supplementation Improved Growth Performance, Antioxidant Status, and Immunity of Broiler Chickens

The global population is estimated to reach approximately 9.7 billion persons by 2050. To meet the demands of the growing population, highly valuable feedstuffs that have traditionally been included as staples in poultry diets (e.g., corn and soybeans) are being reallocated for human nutrition purposes. As the availability of conventional poultry feedstuffs has decreased, poultry nutritionists are investigating the potential use of nonconventional feedstuffs in poultry diets. The objective of this study was to investigate the effects of utilizing dried sweet orange (Citrus Sinensis) peel powder (with and without xylanase supplementation) as a nonconventional feedstuff on production and health parameters in broiler chickens. A total of 375-day-old male Cobb 500 chicks were weighed and blocked for their initial body weight and randomly assigned to 25 pens (15 birds/pen). Dietary treatments were randomly and independently applied to each pen (5 replicates/per treatment). The following five dietary treatments were investigated in this study: 1) CON: basal corn-soya diet, 2) OPP3: basal diet containing 3% orange peel powder, 3) OPP5: basal diet containing 5% orange peel powder, 4) OPP3ENZ: OPP3 diet with xylanase enzyme added (50g/metric ton) and 5) OPP5ENZ: OPP5 diet with xylanase enzyme added (50g/metric ton). Feed intake and body weight were recorded on a weekly basis. Blood samples were collected at the end of the trial and analyzed for antioxidant and immune-related metabolites. The weights of internal organs (including immunological organs) were collected and reported. The data were statistically analyzed using PROC MIXED in SAS (SAS 9.4). Regardless of xylanase supplementation, the OPP-fed birds had decreased feed intakes and improved feed efficiency than the birds that received the control treatment (P=0.01). Significantly reduced Malondialdehyde (MDA) levels in the blood in all OPP-fed groups (P=0.01) compared to the CON group, but relative thymus weight was increased in the OPP5ENZ group. In conclusion, the inclusion of OPP in the broiler chicken diet (up to 5% of DMI) with xylanase enzyme supplementation could improve antioxidant capacity and cellular immune responses, positively impacting production.

Biotreatment of Selected Agrowaste Products with Some Culinary-Medicinal Mushrooms Enhances Their Nutrient Composition for Use as Animal Feed in Nigeria

Biological treatment of agrowaste products using white-rot fungi can enhance their physicochemical and nutritional values for further use as animal feed. In this study, sweet orange and ripe plantain peels were biotreated during their use as substrates to grow three mushroom species (Lentinus squarrosulus, Pleurotus pulmonarius, and P. ostreatus), with the aim to reuse these wastes for animal feed. The effects of mushroom biotreatment on these wastes were analyzed at 0, 30, and 60 days of incubation with respect to physical and chemical properties of the substrates. Results from the mineral composition revealed the presence of appreciable quantities of sodium, magnesium, phosphorus, nitrogen, calcium, potassium, and iron for both peels after treatment with all of the mushrooms. The amino acid content of orange peels treated with L. squarrosulus and P. pulmonarius increased after treatment compared with the control and plantain peel. The carbohydrate level in both wastes also increased after treatment with L. squarrosulus, P. pulmonarius, and P. ostreatus, respectively, while the moisture and ash contents of treated wastes decreased as the incubation periods progressed. However, there was no significant difference (P ≤ 0.05) in the protein, fiber, and fat content of the control compared to those of treated wastes. This study affirms that biotreatment of these agrowaste products using the selected mushrooms can enhanced their value for further use.

Efektivitas Lama Perendaman Serbuk Kulit Jeruk Manis (Citrus sinensis) Terhadap Bilangan Peroksida Pada Minyak Jelantah

Used cooking oil is the remaining cooking oil from the frying process. The use of cooking oil repeatedly at high temperatures and for a long time causes the peroxide number to increase. High peroxide levels can present a health hazard. Phenolic and flavonoid content contained in sweet orange peel is an antioxidant that acts as an antidote to free radicals so that it can slow down the oxidation of used cooking oil. The purpose of this study was to determine the effectiveness of the long soaking time of the sweet orange peel powder (Citrus sinensis) on the peroxide number in used cooking oil. This type of research is experimental with the number of sample repetitions for each treatment, namely 25 samples. The formulation of the problem of this research is whether there is an effect of the effectiveness of the long soaking time of the sweet orange peel powder (Citrus sinensis) on the peroxide number in used cooking oil. From the results of the examination, the average levels of peroxide numbers in used cooking oil that had been soaked were 1 day 22.7949 mEq, 2 days 19.5965 mEq, 3 days 13.5975 mEq and 4 days 9.5984 mEq. Statistical analysis using ANOVA showed the effect of the effect of soaking duration of sweet orange peel powder (Citrus sinensis) on the peroxide number in used cooking oil p (sig) = 0,000 which is less than 0.05. Keywords: Sweet Orange, Peroxide Number, Used Cooking Oil.

The Effect of Solvent Type on Oil Yield on Essential Oil of Sweet Orange Peel Extract (Citrus X Sinensis)

Introduction: Citrus fruits can be processed into various types of preparations that are very useful. Sweet orange (Citrus X sinensis) is a type of orange that is consumed by squeezing it into a drink. So far, sweet orange peels (Citrus X sinensis) have not been fully utilized and become waste that is thrown away. Meanwhile, the peels of citrus fruits have useful content, so that the peels of citrus fruits can be processed into products that are useful for the community. Wet sweet orange peel is better to use than dried sweet orange peel. This is because the sweet orange peel contains essential oils which have not completely evaporated so that the oil can be taken directly.
 Method: This research is a laboratory-based experimental study.
 Results: From the analysis, it was found that the water content of sweet orange peel was 75.5%. The results of data analysis showed that the highest oil yield was obtained using the soxhletation method for 12 hours using 6.15% hexane as a solvent, 4.89% ethanol as a solvent, and 1.67% aquadest as a solvent. When compared to these three solvents, hexane solvent is more effective than ethanol and aquadest. It shows that in general the essential oil from lime peel produced is still within the range of essential oil quality standards based on ISO 3519:2005.
 Conclusion: In this study, the best solvent was hexane. The sweet orange peel essential oil produced has a greenish yellow color for hexane and aquadest solvents and yellowish green for ethanol solvents. The essential oil produced has a density of 0.8580 g/cm3.

Safety and efficacy of feed additives consisting of expressed sweet orange peel oil and its fractions from Citrus sinensis (L.) Osbeck for use in all animal species (FEFANA asbl).

Following a request from the European Commission, the EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the safety and efficacy of expressed sweet orange oil and its fractions obtained from the fruit peels of Citrus sinensis (L.) Osbeck, when used as sensory additives (flavourings) in feed and water for drinking for all animal species. The presence of perillaldehyde was identified as a source of potential concern. However, in target species fed citrus by‐products as part of daily feed, the use of the expressed orange oil and its fractions was not expected to increase the exposure to perillaldehyde to a relevant extent (< 10%). For dogs, cats, ornamental fish and ornamental birds not normally exposed to citrus by‐products, no conclusion can be drawn. For the other species, the FEEDAP Panel concluded that the additives under assessment are safe at the maximum proposed use levels in complete feed. The FEEDAP Panel considered that the use in water for drinking is safe provided that the total daily intake of the additive does not exceed the daily amount that is considered safe when consumed via feed. No concerns for consumer safety were identified following the use of the additives up to the maximum proposed use level in feed. The additives under assessment should be considered as irritants to skin and eyes, and as skin and respiratory sensitisers. The use of the additives under the proposed conditions of use in animal feed was not expected to pose a risk for the environment. Expressed orange oil and its fractions were recognised to flavour food. Since their function in feed would be essentially the same as that in food, no further demonstration of efficacy was considered necessary.

Influence of sweet orange peel oil additive on physicochemical properties of gasoline

The effect of a sweet orange peel oil additive on fuel blends of low-octane gasoline was investigated. The additive contains limonene, β-myrcene, eugenol, and triacetin, which have characteristics resembling gasoline but with advantages in terms of molecular structure that can increase RON and produce a homogeneous mixture. The additive changed the molecular behaviors influencing the physicochemical properties of the fuel blends. The experiments were conducted using fuel blends with additive fractions of 0%–75% (v/v). The fuel blends were mixed manually in 50-ml volumes in closed beaker glasses under STP conditions. The physicochemical properties were then measured to evaluate the chemical molecular structure compositions, viscosities, densities, heating values, and RONs of the blends, to determine the effect of the fuel additive on engines.The results show that the additive compounds were able to change the molecular behaviors of the fuel blends. The fuel blends produced 50 hydrocarbon compounds from 32 pure gasoline hydrocarbon compounds, which influenced the physical properties of the blends. The viscosities, beyond the lowest value at approximately 1.8 cSt, exhibited an increasing pattern compared to the viscosity of base gasoline fuel. The density and heating values tended to increase, even though there were fluctuating values due to intramolecular force movement and differences in molecular dipoles in the fuel blends. The RONs of the fuel blends, with additive fractions of 1%, 5%, 10%, 25%, 50%, and 75%, increased to 88.2, 91.5, 92.1, 95.7, 103.9, and 120.45, respectively. The increase in RON was also affected by the additive components among the fuel-blend molecules, which reacted to form highly stable hydrocarbon molecules, particularly aromatic and branched hydrocarbons, that inhibited combustion at low temperatures. Therefore, sweet orange peel oil as an additive can improve the physicochemical properties of gasoline, and thus can be mixed with any commercial fuel, especially those with a low octane number. It can be used for engines that require standard physical and high-octane properties.

Nano-formulations of hesperidin and essential oil extracted from sweet orange peel: Chemical Properties and Biological Activities

Essential oils and Flavonoids are widely recognized for their beneficial effects in possessing many biological activities, such as antioxidant, antimicrobial and cytotoxic properties, also using in food additives and in the cosmetic industry. The aim of this work is to produce nanoparticles of hesperidin and essential oil extracted from waste orange peels, and study its biological impact compared to native products (hesperidin and essential oil) as antioxidant, antimicrobial and cytotoxic. Obtained products (nano-formulation) are characterized by zeta potential, electron scanning microscopy, spectrophotometric method and color analysis. The final products (nano-formulation) were examined as antioxidant agent by two assays (DPPH and ABTS analysis). The antioxidant activity of nano hesperidin and essential oil was evaluated and compared with none formulated. Nanoparticles of hesperidin and essential oil have a great potential in DNA damage prevention compared to their respective controls which were examined by DNA damage assay induced by oxidative stress protection analysis. The in vitro cytotoxic activity of nano formulated hesperidin and essential oil, towards (three cancerous cell lines, including: breast (MCF-7), colorectal (HCT-116) and liver (HepG2), and two non-cancerous control cell lines (fibroblast BJ-1and MCF-12F) compared with native product (essential oil and hesperidin) were examined using MTT assay. The hesperidin and essential oil NPs showed a positive effect on the inhibition of the proliferation of all tested cancer cell line. The antimicrobial activity of hesperidin, essential oil and their nano formulation form was evaluated using well diffusion assay. The nano formulation form of hesperidin and essential oil showed to improve the antimicrobial activity. These findings showed that nano-hesperidin and nano - essential oils play an important role as antioxidant, antimicrobial and cytotoxic agent's effects. These effects might be used for clinical trials and can represent driving formulation for novel chemotherapeutic agents and in food industry. Further studies about the therapeutic effects of nano-hesperidin and essential oil extracted from Egyptian orange peel are requested

Pengaruh Penambahan Bubuk Jahe Emprit (Zingiber officinale var. Amarum) Terhadap Karakteristik Teh Herbal Celup Kulit Jeruk Manis (Citrus sinensis L.)

This study aimed to determine the effect of emprit ginger powder (Zingiber officinale var. Amarum) on the characteristic of sweet orange peel herbal tea (Citrus sinensis L.) as well as the appropriateness of emprit ginger powder for the best characteristic. This study was designed using an experimental method which is Completely Randomized Design with the treatment of adding emprit ginger powder (0%, 5%, 10%, 15%, 20%) that was repeated 3 times to obtain 15 experimental units. Observed variabels were water content, extract water content, vitamin C, total phenolic, total flavonoid, antioxidant activity, scoring test, and hedonic test. The obtained data were analyzed by using the analysis of variance. If it had a significant effect, it was continued to be tested by using the Duncan Multiple Range Test. The results showed that the addition of emprit ginger powder had a very significant effect on the water content, vitamin C, total phenolic, total flavonoid, antioxidant activity, aroma (scoring and hedonic test), taste (scoring and hedonic test), and overall acceptance, while extract levels in the water had a significant effect and color (hedonic test) had a non significant effect. Furthermore, it showed that the addition of 20% emprit ginger powder extract was the best treatment with the characteristic as follows: 8.13% w / w water content, 26.93% w / d extract water content, vitamin C 25.56 mg / g tea powder, total phenol 17.86 mg GAE / g tea powder, total flavonoids 1.17 mg QE / g tea powder, and antioxidant activity 56.89%. The characteristics of the tea brewing water involves the distinctive aroma of ginger emprit which is very liked, the color is liked, the taste is not bitter and liked, and overall acceptance is liked.

Isolation and Identification of Essential Oil Made From Kisar Sweet Orange Peels

Kisar sweet orange is an endemic fruit and primary commodity from Kisar Island, Southwest Maluku Regency - Maluku Province. Currently, Kisar sweet orangeswere only consumed as fresh fruit during this time, partially processed into juice and the peels were discarded as wastes. Kisar sweet orange peels can be processed into essential oil to increase their economic value. The essential oil made from orange peels is highly preferable, especially for the middle to upper-class consumers, for health and air-freshening purposes. This essential oil has a higher selling value. This research aims at isolating and identifying the chemical components and quality of essential oil made from Kisar sweet orange peels. This essential oil was isolated using a steam distillation method, and its chemical components were identified using GC-MS instruments.. The research results showed that the essential oil made from Kisar sweet orange peels dried with a cabinet dryer for two days utilizing the steam distillation method provides a yield of 0.53%, with its properties of specific gravity of 0.8457, refractive index of 1.4651 (20°C), acid number of 1.92, ester number of 8.05, and solubility in alcohol of 1:9. The main chemical component of the essential oil made from Kisar sweet orange peels detected by GC-MS is limonene (70.80%).

A pattern recognition method on smartphones for planar chromatography and verification on chromatograms of four herbal medicines from citrus fruits

Image retrieval techniques in combination with support vector machine (SVM) on smartphones were studied to make pattern recognition of planar chromatograms. A micellar TLC method employed less toxic and flammable solvents was studied to discriminate four herbal medicines (HMs) from citrus fruits. And the chromatograms were used to test the pattern recognition method. The TLC method employed O/W microemulsion–formic acid (98:2) as the mobile phase, polyamide as the solid phase, and 1% aluminum trichloride aqueous solution as the visualization reagent. TLC of samples of dried lemon peel, dried sweet-orange peel, pummelo peel, and mandarin orange peel of varying origins were performed in three separate runs. Image retrieval techniques combined with SVM directly classified the chromatograms based on migration path images. A mobile application running on an Android smartphone was developed to perform the pattern recognition. The micellar TLC method satisfactorily characterized the chemical profile of the four HMs from citrus fruits. The pattern recognition method combining color layout descriptor with SVM correctly classified all the chromatograms. A smartphone with an application combining color layout descriptor with SVM would be a convenient tool for pattern recognition of planar chromatograms.

Micronization and coating of bioflavonoids extracted from Citrus sinensis L. peels to preparation of sustained release pellets using supercritical technique

Bioflavonoids such as hesperidin and hesperetin as anticancer and antioxidant agents are widely used in the formulation of many herbal drugs. Recently, promising evidence for the prevention and treatment of COVID 19 using these compounds has been reported. Unfortunately, these compounds are often insoluble in aqueous media and have low stability in gastric acid environment. These factors reduced the bioavailability of related oral dosage forms in digestive system. Reduction of particle size and controlled release delivery system can decline the mentioned problems. In the presented research, extraction of bioflavonoids from Citrus sinensis L. (sweet orange) peels was performed using supercritical carbon dioxide. Experimental central composite design was employed for determination of optimal conditions. Qualification analysis was performed using LC–MS, and hesperidin and hesperetin were identified in herbal extract. Micronization and coating of the flavonoids nanoparticles on sugar spheres have been performed using an inhouse developed supercritical technique. Characterization of coated nanoparticles was done using FESEM and ImageJ software. The in vitro antioxidant activity of coated samples was investigated by ferrous ion chelating activity. According to the extraction results, concentration of ethanol as organic co-solvent has the greatest effect on the extraction yield. Analysis of FESEM images illustrated that nanoparticles of extract components with particle size distribution of 5–100 nm were coated successfully. The solubility and antioxidant activity of nanoparticles in aqueous media have increased significantly compared to original form. This process led to increasing dissolution rate and improving the oral bioavailability of flavonoids.Graphical abstract

Preparation of Poliblend Suweg Starch-Chitosan with Addition of Essential Oil from Sweet Orange Peel as Edible Coating on Malang’s Apples

Poliblend edible coating of suweg starch-chitosan (SSC) was prepared as a package with the addition of essential oil from sweet orange peel as antibacterial because of the high limonene content (95%) to increase the shelf life of malang’s apples. There are 6 variations formulation of the suweg starch: chitosan, namely 10: 0, 9: 1, 8: 2, 7: 3, 6: 4 and 5: 5 in total volume 60 mL. Essential oil of sweet orange peel was added with variations in the concentration of essential oil is 0%; 2%; 4%, and 6%.). Furthermore, characterization was carried out by mechanical testing, Water Vapor Transmission Rate (WVTR), solubility, and swelling of the SSC polyblend edible film and the antibacterial activity test of the SSCO polyblend edible coating on Staphylococcus Aureus bacteria before it was applied on malang’s apples. Organoleptic tests and physical observations of malang’s apples were carried out after the application of edible coating SSCO. The result characterization of the functional groups of polyblend edible film SSCO showed the absorption of the wave number 3373.64 cm−1; 1646.32 cm−1; 1570.12 cm−1; 1416.78 cm−1 and 1337.69 cm−1; and 1040.64 cm−1 with functional groups-OH, N-H, C=C, CH3, and C-O. The variation composition affects on the physical properties of edible film SSC in the mechanical test, WVTR, and WVP with the optimum concentration edible film SSC 7: 3, while the solubility and swelling reach the optimum concentration edible film SSC of 5: 5. The addition of sweet orange peel as essential oil had an effect on the antibacterial activity of polyblend edible film SSCO with a maximum inhibition zone 16.55 mm on concentration 6% for S. aureus bacteria. Based on organoleptic test, consumers prefer edible coating SSC with the lowest essential oil content and malang’s apples can last 22 days on physical performance observations.

Electrical Analysis of Combination of Orange Peel and Tamarind for Bio-battery Application as an Alternative Energy

The use of disposable primary batteries causes environmental pollution due to the lack of battery waste treatment facilities, so it is necessary to handle the problem by making bio-batteries from orange peel and tamarind. The purpose of this study is to know the influence of good electricity on variations of the combination of orange peel and tamarind. The novelty of this study is (1) the use of biomass combination of orange peel and tamarind as bio-battery electrolytes and (2) comparison of orange peel to tamarind. The method used in this study is by experimenting with a mixture of sweet orange peel and tamarind made into pasta and put in a used battery. The results showed that bio-batteries with a variation of 25% orange to tamarind had the highest voltage and the most powerful electric current. This happens because the ion content in the variation is not concentrated so it is easy to move widely. In conclusion, bio-batteries with a mixture of orange peel and acid are the best variation of 25% orange peel against tamarind. The study is expected to reduce the use of chemicals in batteries and reduce disposable battery waste.

The effect of sweet orange peel addition into premium gasoline on fuel consumption and flue gas emissions

This study offered an investigation as to how the use of sweet orange peel oil as an additive for premium gasoline can increase octane performance which influence combustion. Sweet orange peel oil-acting as a RON booster-contains monoterpene compounds and oxygenated hydrocarbons which can improve the quality of combustion. The study also aimed to analyze the combustion characteristics of premium gasoline mixture affected by sweet orange peel additives on SI engines. This study carried out tests on an engine fuel consumption and HC emissions. The cyclic rings and methyl groups of monoterpene compounds in sweet orange peel additives contribute to the increase of electronegativity of additives, therefore being able to interfere with the premium gasoline’s compounds which are dominated by aromatics hydrocarbons. It has an impact on the branches of single-bond molecules series of premium gasoline. This makes the premium gasoline cannot be ignited at low temperatures, which results in perfect combustion. In addition, the polarity of triacetin and eugenol, which includes oxygenated hydrocarbons, is able to reduce CO as the avoided gas in combustion. Sweet orange peel additives—processed by maceration extraction method—are added to premium gasoline. There are 3 ratios of additives in this study; orange peel essential oil extracted with a concentration ratio of dried orange peels simplicial to n-hexane (v / v) of 1% (EO A); 0.75% (EO B); and 0.6% (EO C). To determine the effect of the additive, exhaust gas emission testing and fuel consumption measurement were carried out on the 2013 Honda Absolute Revo motorcycle 4-step, SOHC, 1 cylinder, 109.17 cc engine. The exhaust gas, i.e. CO, HC, CO2, and O2 were analyzed using a Stargas-898-type gas analyzer. Furthermore, fuel consumption measurement was carried out at 3500 rpm engine speed. The results show that CO could be reduced by 37.7%, while the remaining O2 was reduced by 17%. The addition of sweet orange peel additives could increase CO2 levels in exhaust gases when compared to premium gasoline combustion without additives. However, the premium gasoline with additive A (EO A) blend tends to produce higher hydrocarbon emissions compared to other types of premium additive proportions. In conclusion, adding sweet orange peel additives to premium gasoline can reduce fuel consumption by 3.3%; 3.2%; and 3.4% for each blend, respectively.

Antioxidant and antimicrobial activities and UPLC-ESI-MS/MS polyphenolic profile of sweet orange peel extracts

With growing consumer awareness, exploitation of renewable resources is cost-effective and environment friendly. This work examines the potential of citrus peels as natural antioxidants and antimicrobials for food preservation. Extraction yield, total soluble phenols and flavonoids of various citrus peels (sweet orange, lemon, tangerine and grapefruit) were optimized by varying the solvent type. While the highest extract yield (~16 ​g/100g) was obtained from the sweet orange peels in methanol, extraction with ethanol maximized the concentration of total phenols and flavonoids (~80 ​mg catechol equivalents/100 ​g dry weight). In addition, sweet orange peel extract showed the highest DPPH, ABTS and hydroxyl radical scavenging values. UPLC-ESI-MS/MS analysis of aqueous and ethanolic extracts of sweet orange peels revealed more than 40 polyphenolic compounds including phenolic acids and flavonoids, some of which have not been previously reported. The predominant polyphenols were narirutin, naringin, hesperetin-7-O-rutinoside naringenin, quinic acid, hesperetin, datiscetin-3-O-rutinoside and sakuranetin. The incorporation of sweet orange peel extract into two vegetable oils enhanced their oxidative stability. In addition, all citrus peel extracts possessed high antimicrobial activity against several food-borne pathogens, and the activity was highest for the sweet orange peel extract. Overall results suggested the great potential of sweet orange peels as natural antioxidant and antimicrobials, which can be efficiently extracted using a simple and low-cost method, for enhancing the storage stability and safety of vegetable oils.

Growth performance, blood parameters and production cost of broiler chickens fed dietary sweet orange peel meal diets with and without enzyme addition

The growth performance, blood parameters and production cost of two hundred and fifty two (252) Abor acre plus broiler chickens fed sweet orange peel meal (SOPM) with and without enzymes were investigated. The experimental diets consist of control (T ), maize was 0 replaced with SOPM at 15, 20 and 25 % without exogenous enzymes to form T , T and T 1 2 3 respectively, and another supplemented with exogenous enzyme to form T , T and T 4 5 6 respectively. The birds were randomly allocated to seven treatment groups replicated three times to have 12 birds per replicate and were fed experimental diet ad-libitum for eight weeks. Result showed that broiler chickens fed control diet (T ) had significantly (P&lt;0.05) 0 higher final weight (FW) of 2.44kg, weight gain (WG) of 42.91, feed intake (FI) of 86.53, better feed conversion ratio (FCR)of 2.01 and protein conversion ratio (PCR)of 0.44 compared to other dietary treatments. Broiler chickens fed T and T had significantly 1 4 (P&lt;0.05) higher final weight of 1.98 and 1.98 kg, weight gain of 34.69 and 34.71 g and feed intake of 78.35 and 81.63 g respectively while significantly (P&lt;0.05) least values of 1.75 and 1.80 kg final weight, 30.73 and 31.49 g weight gain and 69.47 and 71.84 g feed intake respectively were recorded in broiler chickens fed T and T . Haematological indices namely: 2 5 PCV, RBC, WBC and Hb were not significantly different (P&gt;0.05) but MCV, MCH, MCHC, lymphocytes, heterophil, eosinophil and monocytes differed significantly (P&lt;0.05). Serum biochemical indices namely total protein, albumin, globulin and uric acid were significantly (P&lt;0.05) influenced by the experimental diets. Though, there was no detrimental effect of SOPM and enzyme on production cost as profit was made at all replacement levels of SOPM with and without enzyme treatment, but profit per bird (N) and cost to benefit ratio revealed the superiority of the broiler chickens fed control diet (T ). Based on the results obtained, 0 SOPM can replace maize up to 25 % without posing any deleterious effect on the broiler chickens growth performance, blood parameters and production cost. However, the cost to benefit ratio was compromised with the use of SOPM.

Effects of high levels of water soaked sweet orange peel meal on the Performance response and blood profile of West African dwarf goats

A total of sixteen West African grower bucks of about 7-10 months were used in a completely randomised design to assess the effect of feeding diets with high levels of water soaked sweet orange peel meal (WSSOPM) on the performance response and blood profile of West African dwarf goats. Collected sweet orange peels were soaked in water and drained after 24 hours. The peels were then sun-dried on concrete floors for 48hour and afterwards crushed into a meal using a feed milling machine. The WSSOPM was used to compound four(4) experimental diets containing 0%, 60%, 80% and 100% WSSOPM, these were designated treatment T1, T2, T3, and T4, respectively. Each treatment had four (4) replicate. The study lasted for 11weeks. Results showed that, the mean daily feed intake, mean daily weight gain and the feed conversion ratio of the goats did not show treatment effect (P&gt;0.05) among the treatments. The cost analysis showed that profitability increased with increasing levels of WSSOPM in the diets. The blood parameters measured did not show treatment effect, except the albumin values in which T2 (3.53g/dL) was higher than T1 (2.80 g/dL), T3 (2.68 g/dL) and T4(2.55 g/dL).The mineral indices measured showed variations (P&lt;0.05) in only potassium values, treatment effect was not apparent on the serum calcium, phosphorus, magnesium, sodium and zinc values. Water soaked sweet orange peel meal can be used to replace maize offal at 60%, 80% and 100 % in the diets of West African dwarf goats without negatively affecting both performance and blood indices.

Comparative study of extraction yield and antioxidant property of sweet orange peels (Citrus Sinesis) essential oil

The research evaluates the extraction yield and antioxidant potentials of essential oil (EO) of sweet orange peels using pressurized liquid extraction (PLE), Soxhlet (Sox) and hydro distillation (HD). The extracts were investigated to find out the antioxidant properties using 2, 2 -diphenyl-1-picryl-hydrazyl (DPPH) and 2, 2 azino-bis (3-ethylbenzothiazoline-6-sulfonate) radical (ABTS•+). PLE and Soxhlet extracted essential oil showed additional polyphenol compounds and tannins using thin layerchromatogram (TLC) and chemical analyses,respectively. Hydrodistillation indicating a pure essential oil without identified tannins and polyphenols with the highest ABTS activity compared to other produced essential oils of PLE and Soxhlet. The major chemical constituents of the pure essential oil were identified by gas chromatography-mass spectrometry (GC-MS) and they include limonene (90.72%), myrcene (2.82%) and octanol acetate (1.24%). PLE had moderate high yield within short extraction time and the highest antioxidant (DPPH) and can be adjusted to individual materials to maximize the extraction yield and antioxidant property.

Effects of dried water melon and sweet orange peel (dwmop) meal mixture on the haematological and serum indices of growing rabbits

The objective of the present study was to evaluate effects of dried water melon and sweet orange peel (dwmop) meal mixture on some haematological and serum indices of growing rabbits. A total of sixty rabbits 7-8 weeks old with an average weight of 630 – 645 g were randomly divided into five (5) dietary treatments with three (3) replicates and four rabbits per replicate in a Completely Randomized Design (CRD). The dietary treatments include a control diet with no DWMOP in (T1), T2 (5.0% DWMOP), T3 (10.0 % DWMOP), T4 (15.0 % DWMOP) and T5 (20.0 % DWMOP) respectively. Feed and water were offered ad libitum throughout the experiment which lasted for 12 weeks. Data obtained were used to evaluate the haematological parameters (PCV, RBC, Hb, MCV, MCH , MCHC, WBC and its differentials ), some serum biochemical indices (Albumin, globulin, total protein, creatinine, bilirubin, AST and ALT). All the haematological parameters measured were significantly (P˂0.05) different among the treatments. Total protein, bilirubin and creatinine values were not significantly (P˃0.05) influenced by DWMOP, however, glucose level and activities of ALP and AST were significantly (P˂0.05) affected by DWMOP. It was concluded that inclusion of DWMOP at 20 % in the diet of rabbits does not have any deleterious effect on the health status of the animal.