Assessing the efficiency of nitrogen application is a valuable first step in establishing an optimum N fertilizer management plan. The aim of this study was to elaborate a sustainable N management system for sown grassland installed in temperate regions, through manipulation of nitrogen use efficiency and nitrogen response efficiency (NRE) indicators. Research was performed in western Romania. The biological material consists in two forage species of major importance for the forage base worldwide, namely Trifolium pratense L. and Lolium multiflorum Lam., in pure stands and in mixture. Both forage species were sown at two different spacings between rows (12.5 and 25.0 cm) and were fertilized with nitrogen (N) in three doses (N0-control, N50-fertilized with 50 kg N ha−1 and N100-fertilized with 100 kg N ha−1). The studies were made in 2016 and 2017, years very different from each other in matter of rainfalls. The results revealed a different behavior of the three sward types analyzed: while in Trifolium pratense monoculture and in the mixture of the two species studied, less N was removed than was applied; on the other hand Lolium multiflorum monoculture used a high proportion of the N applied, being at the limit to access N from the organic pool or residual N from fertilizer applications to a preceding crop.