Swan Estuary Research Articles

Analytical solutions for the advection-dispersion model for radon-222 production and transport in shallow porewater profiles

Quantifying the water flux between surface water (SW) and groundwater (GW) bodies is important for determining water balances and understanding controls on surface water quality and sustainable allocation of water resources. Methods that quantify water exchange at point scales are highly desirable due to the heterogeneous nature of SW-GW connectivity. Porewater radon-222 (222Rn) profiles can be easily collected and variations of 222Rn activity within the sediments below SW bodies can be used to infer water flow and residence times. The current models used to interpret these profiles assume downward flows, limiting their use in gaining, or dynamic environments. We investigate the use of a new analytical solution based on a finite solution of the advection dispersion equation to predict bi-directional water fluxes using 222Rn profiles in riverbed sediments. We apply this solution to profiles collected from the Swan Estuary in Western Australia. The estimated fluxes of water from the estuary to groundwater were up to 2.07 m/day, and fluxes from the groundwater to the estuary were up to 1.79 m/day at various times. Estimates made with a previously applied piston-flow model were broadly consistent when predicting fluxes from the estuary to the groundwater; however, the method was unable to predict fluxes from the groundwater to the estuary, as degassing in the surface water meant that all profiles indicated ageing with depth and hence downward flow. The reversal of fluxes was predicted for a period of seasonally high groundwater levels. Our results highlight that not accounting correctly for upward flows can impact predictions of groundwater exchange with surface water bodies such as estuaries. This may lead to errors in water and chemical balances in stream, lake and estuarine environments and sustainable allocation of freshwater resources between ecosystems and human demands.

Distinct Endophytic Bacterial Communities Inhabiting Seagrass Seeds.

Seagrasses are marine angiosperms that can live completely or partially submerged in water and perform a variety of significant ecosystem services. Like terrestrial angiosperms, seagrasses can reproduce sexually and, the pollinated female flower develop into fruits and seeds, which represent a critical stage in the life of plants. Seed microbiomes include endophytic microorganisms that in terrestrial plants can affect seed germination and seedling health through phytohormone production, enhanced nutrient availability and defence against pathogens. However, the characteristics and origins of the seagrass seed microbiomes is unknown. Here, we examined the endophytic bacterial community of six microenvironments (flowers, fruits, and seeds, together with leaves, roots, and rhizospheric sediment) of the seagrass Halophila ovalis collected from the Swan Estuary, in southwestern Australia. An amplicon sequencing approach (16S rRNA) was used to characterize the diversity and composition of H. ovalis bacterial microbiomes and identify core microbiome bacteria that were conserved across microenvironments. Distinct communities of bacteria were observed within specific seagrass microenvironments, including the reproductive tissues (flowers, fruits, and seeds). In particular, bacteria previously associated with plant growth promoting characteristics were mainly found within reproductive tissues. Seagrass seed-borne bacteria that exhibit growth promoting traits, the ability to fix nitrogen and anti-pathogenic potential activity, may play a pivotal role in seed survival, as is common for terrestrial plants. We present the endophytic community of the seagrass seeds as foundation for the identification of potential beneficial bacteria and their selection in order to improve seagrass restoration.

The Contrasting Ecology of Free-Living Nematodes in Macrotidal and Microtidal Estuaries

This paper compares the diversity, distribution and trophic composition of the nematode assemblages in the intertidal sediments of the macrotidal Exe Estuary (UK) and the shallow subtidal sediments of the microtidal Swan Estuary (Australia), the latter having been subjected to long-standing anthropogenic disturbance. Data from studies partially described in earlier papers are analysed in a novel comparative way to demonstrate that any differences are attributable to the effects of their contrasting tidal regimes and not to levels of anthropogenic disturbance. They will thus have generic applicability to other macrotidal and microtidal estuaries and to taxa other than nematodes. Although nematode taxonomic and trophic α diversity in the two estuaries are similar, β diversity and consequently γ diversity are much higher in the Exe as a result of the greater heterogeneity of its benthic habitats. In the Exe, there is a gradual transition in taxonomic composition from the estuary to the coast, whereas the composition within the Swan is more uniform and contrasts sharply with that of the coast. Taxonomic and trophic composition correlate closely with sediment environmental variables in the Exe but not the Swan, in which physico-chemical properties of the overlying water are more important. Significant differences between the nematode faunas in the two estuaries result principally from the relatively greater importance of predatory families in the Exe and of deposit-feeding families in the Swan. Nematode diversity is greater than that of the macrobenthos in both estuaries, and this may be true for other naturally disturbed shallow water environments. The lack of faunistic heterogeneity in microtidal estuaries may account for their relative lack of resilience to anthropogenic disturbances and climate change.

Reappearance of Eumarcia fumigata (G.B. Sowerby II, 1853) (Bivalvia: Veneridae) into the Swan River, Western Australia

ABSTRACTThe venerid bivalve Eumarcia fumigata was a common species in Western Australia (WA) during the Pleistocene, where it was distributed as far north as Shark Bay. It became extinct in WA as the climate changed several thousand years ago but remains common in eastern Australia from southern Queensland to South Australia and Tasmania. The species has recently reappeared in the Swan River, probably due to shipping movements. Of the > 60 marine and estuarine species introduced into WA it is only the third confirmed introduction from eastern Australia, and the first that is a reappearance in the Swan River of a species present in the geological past. The present reappearance of E. fumigata, and the introduction of other species, has been made possible by the removal of a rocky bar at the mouth of the estuary and the creation of more marine conditions in the lower Swan estuary.

Simple shade plots aid better long-term choices of data pre-treatment in multivariate assemblage studies

Shade plots, simple visual representations of abundance matrices from multivariate species assemblage studies, are shown to be an effective aid in choosing an overall transformation (or other pre-treatment) of quantitative data for long-term use, striking an appropriate balance between dominant and less abundant taxa in ensuing resemblance-based multivariate analyses. Though the exposition is entirely general and applicable to all community studies, detailed illustrations of the comparative power and interpretative possibilities of shade plots are given in the case of two estuarine assemblage studies in south-western Australia: (a) macrobenthos in the upper Swan Estuary over a two-year period covering a highly significant precipitation event for the Perth area; and (b) a wide-scale spatial study of the nearshore fish fauna from five divergent estuaries. The utility of transformations of intermediate severity is again demonstrated and, with greater novelty, the potential importance seen of further mild transformation of all data after differential down-weighting (dispersion weighting) of spatially ‘clumped’ or ‘schooled’ species. Among the new techniques utilized is a two-way form of the RELATE test, which demonstrates linking of assemblage structure (fish) to continuous environmental variables (water quality), having removed a categorical factor (estuary differences). Re-orderings of sample and species axes in the associated shade plots are seen to provide transparent explanations at the species level for such continuous multivariate patterns.

Development and validation of fish-based, multimetric indices for assessing the ecological health of Western Australian estuaries

We describe the development of the first fish-based, multimetric indices for assessing and monitoring the health of Australian estuaries, and their application to the nearshore (<2 m depth) and offshore (>2 m depth) waters of the Swan Estuary, Western Australia. Suites of fish community metrics, including measures of species composition, diversity and abundance, trophic structure and life history function, were selected via a novel weight of evidence approach on the basis of their sensitivity to detect inter-annual change in estuarine condition. For each selected metric, seasonally-adjusted reference conditions were established for each spatial management zone of the Swan Estuary using 30 years of standardised historical fish assemblage data. This extensive data set provided a sound basis for determining the ‘best available’ standard of biotic integrity recorded over that time period and thus a reliable benchmark against which the current and future health of the estuary may be assessed and compared. The nearshore and offshore indices were robust to the effects of natural, intra-seasonal variability in environmental conditions, and so provide reliable tools for quantifying and classifying the ecological health of the Swan Estuary and its constituent management zones. The response of the nearshore index to an algal bloom confirmed that it is sufficiently sensitive to quantify ecological health responses to local-scale environmental perturbations and to track the subsequent recovery of the system following their removal. The indices provide managers with a reliable, quantitative method for assessing and communicating the health of the Swan Estuary and, similarly, of other estuaries across south-western Australia.

A method for selecting health index metrics in the absence of independent measures of ecological condition

We describe a novel, weight of evidence-based approach for selecting fish community metrics to assess estuarine health, and its application in selecting metrics for a multi-metric health index for the Swan Estuary, Western Australia. In the absence of reliable, independent measures of estuarine condition against which to test the sensitivity of candidate metrics, objective, multivariate statistical analyses and multi-model inference were employed to select metric subsets likely to be most sensitive to inter-annual changes in the health of this ecosystem. Novel pre-treatment techniques were first applied to down-weight the influence of highly erratic metrics and to minimise the effects of seasonal and spatial differences in sampling upon metric variability. A weight of evidence approach was then adopted to select those metrics which responded most consistently across multiple analyses of nearshore and offshore fish abundance data sets collected between 1976 and 2009. Sets of 11 and seven metrics were selected for assessing the health of the nearshore and offshore waters of the Swan Estuary, respectively. Selected metrics represented species composition and diversity, trophic structure, life history and habitat functions and, in the case of the nearshore index, a potential sentinel species. These metric sets are currently being used to construct a multi-metric health index for the Swan Estuary, which is the first such tool to be developed for assessing the health of estuaries in Australia. More broadly, while the methodology has in the present case been applied to the fish fauna of the Swan Estuary, it is generally applicable to any ecosystem and type of biotic community from which an ecosystem health index might be sensibly derived.

Importance-satisfaction analysis for marine-park hinterlands: A Western Australian case study

Tourist use of national and marine parks continues to increase worldwide. Effective management depends on being able to evaluate the quality of visitors’ experiences, as well as protecting the natural environment. In tourism management, importance-performance analysis (IPA) has been used as part of quality management. It has recently been applied to national park management. This paper re-conceptualises this analysis to one of importance satisfaction, enabling a focus on the quality of experience. Two methods, importance-performance analysis and service quality gap, were modified and applied in the hinterland of Swan Estuary Marine Park in Western Australia. Both provided data useful for evaluating satisfaction, with the choice of method depending on the end user's resources and requirements as well as cognisance of each method's limitations. For most of the Marine Park attributes, satisfaction exceeded importance and hence no management attention is needed. Exceptions were the condition of the Swan River and associated footpaths, and the presence of litter and wildlife. For these, satisfaction was lower than importance, suggesting management attention is needed.

The benthic macroinvertebrate community of the upper reaches of an Australian estuary that undergoes marked seasonal changes in hydrology

AbstractBenthic macroinvertebrates were collected from the substrata of nearshore, shallow waters (depths &lt;1 m) and offshore, deeper waters (depths 2·5–5 m) of three regions in the saline lower reaches of the tributary rivers that comprise the upper Swan Estuary in each season between winter 1995 and autumn 1997. Freshwater discharge in the two tributary rivers varied markedly during both years and consequently salinities in the upper estuary ranged from &lt;1‰ in winter to &gt;30‰ in autumn. The most abundant species, the galeommatid bivalve Arthritica semen, contributed &gt;25% to the total numbers of macroinvertebrates in both shallow and deeper waters. The continuous reproduction, rapid growth, short life cycle and tolerance to a wide range of salinities, that characterize this species, represent ideal adaptations for life in a seasonally very variable environment. The overall number of species and density of benthic macroinvertebrates were typically greater in shallow than deeper waters. Species composition also differed significantly between water depths. The nereidid polychaete Ceratonereis aequisetis and mytilid bivalve Xenostrobus securis, which feed on plant material, were relatively far more abundant in shallow than deeper waters, whereas the reverse was true for the spionid polychaete Prionospio cirrifera and sabellid polychaete Desdemona ornata, which feed on the detrital material that settles out in deep waters. Heavy freshwater discharge in winter 1996 was accompanied by very marked changes in the richness, density and composition of species in shallow waters, but not in those of deeper waters where scouring would have been less pronounced. The species compositions in both the shallow and deeper waters of each region usually differed significantly among the seasons of each year and between the corresponding seasons in the different years. Region within the estuary had little or no influence on the species richness, density and faunal composition of benthic macroinvertebrates, even though the regions were located in two different tributary rivers. The above results demonstrate that, within each region, certain species were particularly successful in the first year, while others were more abundant in the second year, which in turn suggests that the benthic macroinvertebrate fauna of each region is responding to the same fine‐scale differences between environmental conditions in the two years. Copyright © 2001 John Wiley &amp; Sons, Ltd.

Do the dietary compositions of Acanthopagrus butcheri in four estuaries and a coastal lake vary with body size and season and within and amongst these water bodies?

The dietary compositions were determined for Acanthopagrus butcheri in four estuaries and a saline, coastal lake, which vary in the extent, if any, of their connection to the sea and amongst which, in spring and summer, their salinities ranged from 2 to 7‰ in an intermittently open estuary to &gt;40‰ in a normally closed estuary. The dietary compositions of A. butcheri in each of the five water bodies were significantly different, which reflected differences in the abundance of different components of the biota in those systems. Biotic differences amongst water bodies thus accounted for the far larger contributions made to the volume of the stomach contents by the macroalgae Cladophora sp. in the Moore River Estuary on the lower west coast of Australia, by amphipods and decapods in the Swan River Estuary 85 km further south and by polychaetes in the landlocked Lake Clifton a further 85 km further south. The diet of A. butcheri in the Nornalup/Walpole Estuary on the south coast of Western Australia contained atypically large volumes of the seagrass Ruppia megacarpa and teleosts, whereas that in the normally closed Wellstead Estuary, 260 km to the east was characterised by large volumes of the macroalgae Chaetomorpha sp. and a tube‐dwelling amphipod. However, there is evidence that A. butcheri selects certain prey, when two or more of the typical prey of A. butcheri are present in the environment, and that it prefers to feed on or above the substratum, rather than within the substratum. The dietary composition of A. butcheri underwent pronounced ontogenetic changes in each water body, these being progressive in estuaries on the lower west coast and abrupt in those on the south coast. Within the upper Swan Estuary, the dietary composition changed in an upstream direction, reflecting changes in the relative abundance of certain benthic macroinvertebrate prey, but did not undergo conspicuous seasonal changes, which is consistent with the lack of any clear cut seasonal changes in the abundance of their major prey.

Do the dietary compositions of Acanthopagrus butcheri in four estuaries and a coastal lake vary with body size and season and within and amongst these water bodies?

The dietary compositions were determined for Acanthopagrus butcheri in four estuaries and a saline, coastal lake, which vary in the extent, if any, of their connection to the sea and amongst which, in spring and summer, their salinities ranged from 2 to 7‰ in an intermittently open estuary to >40‰ in a normally closed estuary. The dietary compositions of A. butcheri in each of the five water bodies were significantly different, which reflected differences in the abundance of different components of the biota in those systems. Biotic differences amongst water bodies thus accounted for the far larger contributions made to the volume of the stomach contents by the macroalgae Cladophora sp. in the Moore River Estuary on the lower west coast of Australia, by amphipods and decapods in the Swan River Estuary 85 km further south and by polychaetes in the landlocked Lake Clifton a further 85 km further south. The diet of A. butcheri in the Nornalup/Walpole Estuary on the south coast of Western Australia contained atypically large volumes of the seagrass Ruppia megacarpa and teleosts, whereas that in the normally closed Wellstead Estuary, 260 km to the east was characterised by large volumes of the macroalgae Chaetomorpha sp. and a tube-dwelling amphipod. However, there is evidence that A. butcheri selects certain prey, when two or more of the typical prey of A. butcheri are present in the environment, and that it prefers to feed on or above the substratum, rather than within the substratum. The dietary composition of A. butcheri underwent pronounced ontogenetic changes in each water body, these being progressive in estuaries on the lower west coast and abrupt in those on the south coast. Within the upper Swan Estuary, the dietary composition changed in an upstream direction, reflecting changes in the relative abundance of certain benthic macroinvertebrate prey, but did not undergo conspicuous seasonal changes, which is consistent with the lack of any clear cut seasonal changes in the abundance of their major prey.

A review of the Arhythmacanthidae (Acanthocephala) with a description ofHeterosentis hirsutusn. sp. FromCnidoglanis macrocephala(Plotosidae) in Australia

Heterosentis hirsutus n. sp. is described from Cnidoglanis macrocephala (Siluriformes: Plotosidae) from the Swan Estuary, Western Australia. It is distinguished by having 14 longitudinal rows of 6-7 hooks per row on the proboscis, a trunk armed anteriorly and posteriorly (= genital spines) with minute spines and lemnisci that may extend to the posterior margin of the proboscis receptacle. The new species also has prominent fragmented nuclei in its trunk wall. New information is given for Heterosentis plotosi Yamaguti, 1935 from Plotosus lineatus (Siluriformes: Plotosidae) and H. paraplagusiarum (Nickol, 1972) Amin, 1985 from Paraplagusia guttata (Pleuronectiformes: Cynoglossidae), both from Queensland. A key to the species of Heterosentis Van Cleave, 1931 is provided. The Arhythmacanthidae subfamilies are reviewed: there is little utility in the recognition of these taxa because of the small number of genera involved and the validity of the characters on which they are based is in doubt, particularly whether trunk spines are present or absent. Only Acanthocephaloides Meyer, 1932, Breizacanthus Golvan, 1969, Euzetacanthus Golvan & Houin, 1964, Heterosentis, Hypoechinorhynchus Yamaguti, 1939 and Paracanthocephaloides Golvan, 1969 of the Arhythmacanthidae are considered valid. A key to these genera is provided. The monotypic genus Neoacanthocephaloides Cable & Quick, 1954 is considered a new synonym of Acanthocephaloides thus creating Acanthocephaloides spinicaudatus (Cable & Quick, 1954) n. comb. Arhythmacanthus Yamaguti, 1935 is maintained as a synonym of Heterosentis because the distinction between two and three hook types is made equivocal when the transition between the apical and subapical hooks is gradual.

Biannual spawning periods and resultant divergent patterns of growth in the estuarine goby Pseudogobius olorum: temperature-induced?

Samples of juveniles and adults of the goby Pseudogobius olorum were collected from seven sites in the shallows of the upper Swan Estuary, Western Australia, using a 3 mm-mesh seine net on one or two occasions in each month between September 1983 and April 1985. The mean gonadosomatic index of female fish rose from very low values in winter (June–August) to a sharp peak in mid-spring (October), reflecting the rapid maturation of ovaries over, this period. Ovaries with post-ovulatory follicles and ovaries that were undergoing degeneration were present, in November and December, but were then either rare or absent in those members of the corresponding cohort which survived into January and February. Female fish with advanced oocytes and mature ovaries were not found in December and January, but were present in February to April. The above trends exhibited by ovarian maturity indices, together with the appearance of larvae and small fish in both spring and autumn, demonstrate that P. olorum spawns in both spring and autumn and at best to only a limited extent in summer. Length-frequency and gonadal data show that the progeny of the spring-spawning group frequently spawn in the following autumn, when they are ∼ 5 mo old, and that those of the autumn-spawning group frequently spawn in the following spring, when they are ∼ 7 mo old. Some representatives of these two spawning groups survive through the winter and summer, respectively, to breed in a second season. Growth of the progeny of the spring-spawning group was relatively rapid between late spring and mid-autumn, whereas that of the autumn-spawning group was negligible during winter, but then inceased markedly in spring. It is proposed that the biannual spawning periods in each year by P. olorum in the Swan Estuary developed as a result of a rise in water temperature over the last few thousand years. Such a rise would have brought forward further into spring and extended later into autumn the periods when the water temperatures lie within the range (20 to 25°C) at which P. olorum typically spawns. However, mid-summer is now characterised by water temperatures >25°C, which are considered less conducive to reproductive success.

Growth, movements and diet of the terapontid Amniataba caudavittata in an Australian estuary

Samples of Amniataba caudavittata were collected from the Swan Estuary in south-western Australia between February 1977 and December 1980, using seine nets in inshore shallow waters and gillnets and otter trawls in offshore waters. Although fish could only be aged using otoliths until they were 18–22 months old, they had by that time reached c. 75% of their final length. The mean lengths of the cohorts determined by using MIX, which analysed each of the monthly length-frequency distributions independently, were similar to those yielded by MULTIFAN, which constrains the means of each of the sequential and corresponding cohorts to a von Bertalanffy growth curve. Consequently, the von Bertalanffy growth curve parameters determined by MULTIFAN and those derived from the use of the means produced by MIX were similar. The clear cut trends exhibited by the progression of modal lengths in successive samples, and their close correspondence to the trends shown by the growth curves, demonstrate that many A. caudavittata live for at least 3 years. Growth was seasonal and confined to the warmer months of the year. The apparent negative growth recorded in winter can be attributed to a tendency for the smaller representatives of the different cohorts to remain inshore at that time and thus to be more susceptible to capture by seine netting, the main sampling method. Offshore movements by larger fish in the winter allow those fish to remain in the high salinities found beneath the pronounced haioclines that form in the deeper waters of this estuary during the heavy freshwater discharge that occurs typically at that time of the year. Adult A. caudavittata move into the upper estuary where spawning occurs, with considerable numbers of the resultant juveniles then moving downstream into the middle estuary. A, caudavittata is a benthic omnivore, with the 0+ age class ingesting algae and a range of small crustaceans, while older fish prey to a greater extent on polychaetes.

Growth, movements and diet of the terapontid Amniataba caudavittata in an Australian estuary

Samples of Amniataba caudavittata were collected from the Swan Estuary in south‐western Australia between February 1977 and December 1980, using seine nets in inshore shallow waters and gillnets and otter trawls in offshore waters. Although fish could only be aged using otoliths until they were 18–22 months old, they had by that time reached c. 75% of their final length. The mean lengths of the cohorts determined by using MIX, which analysed each of the monthly length‐frequency distributions independently, were similar to those yielded by MULTIFAN, which constrains the means of each of the sequential and corresponding cohorts to a von Bertalanffy growth curve. Consequently, the von Bertalanffy growth curve parameters determined by MULTIFAN and those derived from the use of the means produced by MIX were similar. The clear cut trends exhibited by the progression of modal lengths in successive samples, and their close correspondence to the trends shown by the growth curves, demonstrate that many A. caudavittata live for at least 3 years. Growth was seasonal and confined to the warmer months of the year. The apparent negative growth recorded in winter can be attributed to a tendency for the smaller representatives of the different cohorts to remain inshore at that time and thus to be more susceptible to capture by seine netting, the main sampling method. Offshore movements by larger fish in the winter allow those fish to remain in the high salinities found beneath the pronounced haioclines that form in the deeper waters of this estuary during the heavy freshwater discharge that occurs typically at that time of the year. Adult A. caudavittata move into the upper estuary where spawning occurs, with considerable numbers of the resultant juveniles then moving downstream into the middle estuary. A, caudavittata is a benthic omnivore, with the 0+ age class ingesting algae and a range of small crustaceans, while older fish prey to a greater extent on polychaetes.

Reproductive biology and larval development of the terapontid Amniataba caudavittata, including comparisons with the reproductive strategies of other estuarine teleosts in temperate Western Australia

Samples collected monthly from the Swan Estuary between March 1978 and May 1979, together with environmental data for 1977 to 1980, have been used to elucidate various aspects of the reproductive biology of Amniataba caudavittata in this estuary. The gonads of A. caudavittala started to develop rapidly in the spring, when day length, water temperatures and salinities were increasing markedly. Spawning, which occurred mainly towards the top end of the upper estuary, was initiated in November, when water temperatures and salinities in that region were c. 24° C and 9‰, and it peaked in December/January when they were c. IT C and 17%○, Maturity is attained by at least the majority offish at the end of the second and each subsequent year of life. Although some of the larger 1‐year‐old fish attained maturity, this occurred in only one of the two tributary rivers, possibly reflecting differences in the salinity regimes in these rivers. Fecundities ranged from 50 000 in a 150‐mm fish to 705 000 in a 254‐mm fish, with a mean of 310 000. The mature, unfertilized eggs are small and spherical and have a diameter of 560 μm. The larvae are pelagic and characterized by an elongate body, which becomes moderately deep and laterally compressed during development, a short to moderate, tightly‐coiled gut, a distinct gap between the anus and the origin of the anal fin and 25 or 26 myomeres. The development of fins and settlement of A. caudavittata larvae occurs at a smaller size than in the larvae of other terapontid species previously described. The success of A. caudavittata in the Swan Estuary can be attributed in part to its production of very large numbers of eggs at a time when, due to low fresh water discharge and a smait tidal range, conditions in the estuary are relatively stable. Comparisons between the biology of A. caudavittata and that of other abundant teleosts that spawn in south‐western Australian estuaries show that these species exhibit a wide range of reproductive strategies.

Spatial segregation amongst goby species within an Australian estuary, with a comparison of the diets and salinity tolerance of the two most abundant species

The present study was undertaken to determine whether the various species of gobies that are found within the large Swan Estuary in south-western Australia are segregated within that system, and to attempt to determine the basis for any differences in their spatial distributions. The Swan Estuary comprises a long entrance channel (lower estuary), two wide basins (middle estuary) and the saline reaches of the tributary rivers (upper estuary). A total of 26232 gobies, representing seven species, was collected using a 3 mm-mesh seine net at 15 sites throughout this estuary on at least one occasion monthly over seven consecutive seasons between September 1983 and March 1985. Favonigobius lateralis and Pseudogobius olorum contributed 47.0 and 47.8%, respectively, to the total catch of gobies at all sites. The densities of each species at each site were used to determine the relative contribution of each species to the gobiid fauna at each of the sites in the lower, middle and upper estuary. Comparisons of these data with those published on the distribution and abundance of gobiid larvae confirmed that F. lateralis, which was found predominantly in the lower estuary, is a marine species that spawns in high salinities near the estuary mouth or in inshore coastal waters. In contrast, the life cycle of P. olorum and Papillogobius punctatus are typically completed within the saline reaches of the upper estuary, and that of Arenigobius bifrenatus within both this region and parts of the middle estuary where the substrate is particularly soft. Afurcagobius suppositus also spawns in this area, as well as in fresh water. Tridentiger trigonocephalus, represented by only eight individuals, is an introduced, marine species that was found mainly in the lower estuary. A single representative of the marine species Callogobius depressus was caught. The relatively low numbers of gobies caught in the middle estuary, where they contributed only about 3.5% to the total number of all gobies at all sites, may represent an aversion to the presence of rougher waters in the large basins. Circumstantial evidence suggests that the sandy substrate and consistently high salinities found in the lower estuary are preferred by F. lateralis, whereas the silty surface to the substrate and lower salinities of the upper estuary are preferred by Pseudogobius olorum. Densities of three of the four most abundant species were higher in either spring or summer than in winter, reflecting the influx of 0 + recruits, and possibly also the tendency for species in estuaries to congregate in the shallows during the warmer periods of the year. F. lateralis fed mainly on polychaetes and crustaceans, whereas P. olorum ingested predominantly algae, reflecting differences in mouth morphology and feeding behaviour, rather than the type of food available.

Reproductive biology and larval morphology of the marine plotosid Cnidoglanis macrocephalus (Teleostei) in a seasonally closed Australian estuary

Monthly trends shown by gonadosomatic indices, the prevalence of the different gonadal stages, and the size distribution of the oocytes, indicate that the large marine and commercially important plotosid Cnidoglanis macrocephalus spawns in Wilson Inlet between October and January. The conclusion that spawning occurs within this seasonally closed estuary was confirmed by the presence of males in large nests and by the capture of newly-hatched, yolk sac larvae from one of those nests. The fact that C. macrocephalus, which is also widely distributed in coastal marine waters throughout much of southern Australia, can spawn within Wilson Inlet would be of particular value to this species in those periods when closure of the estuary would preclude a seawards spawning migration. Sexual maturity is size dependent, with spawning rarely occurring before fish have reached a total length of 425 mm. Sexual maturity was attained by a few fish at the end of their second year, by several at the end of their third year and by most, if not all fish, at the end of their fourth year. Comparisons with data for the more northern and permanently open Swan Estuary indicate that C. macrocephalus also spawns within that system and that the spawning time of this species is related to water temperature. The adult male guards the larvae under its pelvic fins in burrows. The larvae increased in total length from 29 mm just after hatching to 43 mm in the 17–18 days after capture, during which time their yolk sac was resorbed. Details are given of the morphology, morphometrics, meristics and pigmentation of larval C. macrocephalus. In comparison with the larvae of three other plotosid genera, the larva of C. macrocephalus is far larger in size and more developed at hatching and takes a shorter time to transform into a juvenile.

Seasonal and spatial changes in the larval fish fauna within a large temperate Australian estuary

A total of 66814 fish larvae, representing 37 families and 74 species, were collected in samples taken monthly between January 1986 and April 1987 from 13 sites located at frequent intervals throughout the large Swan Estuary in south-western Australia. The Gobiidae was the most abundant family, comprising 88.2% of the total number of larvae, followed by the Clupeidae (3.4%), Engraulididae (2.9%) and Blenniidae (1.0%). The most abundant species were Pseudogobius olorum (53.3%), Arenigobius bifrenatus (31.2%) and Engraulis australis (2.9%). Abundance of fish larvae in the lower, middle and upper regions of the estuary each reached a maximum between mid-spring and early summer, 2 to 4 mo before the attainment of maximum temperatures. Larvae of species such as Nematalosa vlaminghi and Apogon rueppellii were collected only between November and February, whereas those of others such as P. olorum, E. australis and Leptatherina wallacei were present over many months. The times and locations of capture of larvae have been related to the distribution and breeding periods of the adults of these species. The mean monthly number of species was far greater in the lower than upper estuary (14.7 vs 2.7), whereas the reverse was true for mean monthly concentration (42 vs 197 larvae per 100 m3). Classification, using the abundance of each of the 74 species recorded at the different sites, showed that the composition of the larval fish fauna in the lower, middle and upper estuary differed markedly from each other. Most larvae caught in the lower estuary belonged to marine species, whereas those in the upper estuary almost exclusively represented species that spawn within the estuary. The fact that the larvae of the 59 species of marine teleosts recorded during this study were restricted mainly to the lower estuary, and yet contributed only 6.2% to the total numbers for the whole estuary, helps to account for the relatively high species diversity in this region. The lack of penetration of many of these larvae beyond the first 12.5 km of the estuary presumably reflects the weak tidal effect in the wide basins of the middle estuary and saline regions of the tributary rivers. The larvae of the 13 teleosts that typically spawn within the estuary contributed 93.8% to the total numbers of larvae. Most of these estuarine-spawned larvae belong to teleosts that deposit demersal eggs and/or exhibit parental care (egg-guarding and oral and pouch-brooding), characteristics which would maximize their chances of retention within the estuary.

Factors influencing community structure and distribution of different life-cycle categories of fishes in shallow waters of a large Australian estuary

Fish were collected at regular intervals over 5 yr (February 1977 to December 1981) from ten shallow-water sites located throughout the lower, middle and upper regions of the large Swan Estuary in temperate southwestern Australia. Analysis of the catch data showed that the total number of species and total density of fishes were both influenced to a greater extent by site and season within the estuary than by year. The number of species and density of fishes within the whole system were greatest during the summer and autumn, when salinities and temperatures were at a maximum, and declined with distance from the estuary mouth. This reflects the trends shown by marine species, which comprise many species that occur only occasionally in the estuary (marine stragglers) and others which enter estuaries regularly and in considerable numbers (marine estuarine-opportunists). The density of marine estuarine-opportunists was also correlated with temperature, reflecting the tendency for the species of this category to congregate in the shallows during the summer and autumn. The similar seasonal aggregations of the single anadromous species and representatives of species that complete their whole life cycle in the estuary were frequently related to spawning. The density of the estuarine category was correlated neither with salinity nor distance from the estuary mouth. The number of estuarine species was also not correlated with distance from the estuary mouth. The density of freshwater species was inversely correlated with salinity and positively correlated with distance from the estuary mouth. The composition of the fish fauna changed progressively through the estuary, with that of the lower estuary being the most discrete. The composition also changed seasonally, particularly in the upper estuary where, during the winter and spring, the volume of freshwater discharge increased greatly and as a consequence the salinity declined markedly. The species diagnostic of the lower estuary were generally marine estuarine-opportunists, whereas those of the upper estuary typically belonged to either the estuarine or anadromous categories. The marine estuarine-opportunistMugil cephalus was, however, also one of the diagnostic species in the upper estuary during the winter and spring.