Sustained Input Research Articles

Organophosphate esters in terrestrial environments of Fildes Peninsula, Antarctica: Occurrence, potential sources, and bioaccumulation

Despite growing concerns regarding the long-range transport (LRT) and ecological risks of organophosphate esters (OPEs), information on the environmental behaviors of OPEs in polar terrestrial ecosystems remains inadequate. In the present study, 10 OPEs were analyzed in soil and vegetation samples collected from Fildes Peninsula, Antarctica. The OPE concentrations in Antarctic soils, mosses, and lichens ranged from 0.87 to 15.7 ng/g dry weight (dw), 9.8 to 113 ng/g dw, and 3.6 to 75.2 ng/g dw, respectively. Non-chlorinated OPEs predominated in terrestrial matrices, accounting for approximately 76 % of the OPE composition. Source identification indicated that OPE contamination in Antarctica likely resulted from local anthropogenic sources and LRT. Moreover, the bioaccumulation behavior of OPEs from soil to vegetation was assessed using bioconcentration factors (BCFs), revealing a significant non-linear trend of initial increase and subsequent decrease in BCFs relative to the lipophilicities of the octanol-air partition coefficient (log KOA) and octanol-water partition coefficient (log KOW). While low levels of OPEs in Antarctic terrestrial environments were reported in this study, their sustained inputs and potential ecological risks in polar regions warrant further attention.

IJCM_207A: Deaf blindness in India: Prevention and Long-Term Challenges

Background: Deaf-blindness is a disability encompassing varying degrees of hearing impairment and low vision together. Congenital deafblindness is when its onset under 2 years age. Objectives: In India, there are no organized data for deafblindness and estimates indicate there could be more than 500,000 deafblind people. Voluntary organizations provide unwavering support to deafblind children and adults alike. Methodology: (i) early identification (ii) prompt referral for community rehabilitation (iii) early assessment and intervention by identification of high- risk children (iv) individualized education to monitor progress (v) continued review and planning for further education and employment in adulthood. Results: Primary prevention by (i) immunization against Rubella to prevent Congenital Rubella syndrome; (ii) education of all women of childbearing age about healthy habits and avoiding alcohol and drug use; and (iii) accessible antenatal health care for all pregnant women. Conclusion: Strategists and politicians need joint up thinking with perseverance and a strong will to tackle this condition with long term sustained input. They are urged to consider deafblindness as a distinct category in the national census and survey counts.

Long-term changes in water quality downstream of three abandoned metal mines

Abandoned metal mines pollute rivers with both acidic and circumneutral metal-rich waters, sometimes from well-mapped mines, but often from long-abandoned adits with limited historical records. Changes in water quality over the monitored timescales were calculated for three mines across Wales (Nantymwyn, Parys Mountain and Frongoch), each abandoned since the early 20th century. Using all available spot sampling data provided by the environmental regulator, it was found that there was a sustained input of metals to the receiving watercourses with no or limited reduction in the concentrations from the mines without remediation, despite the passage of time and impacts of weathering. At Nantymwyn, comparison with five months’ sampling in 2019 showed no significant change (p > 0.05) in metal concentrations compared to the same months in 1985. Parys Mountain showed an increase in filtered zinc concentrations of 2 % between 2004 and 2020. At Frongoch, however, a substantial reduction in its environmental impact was observed after remediation, including a 90 % reduction in total lead concentrations. Realisation that the pollution from these sites does not reduce over the decades since abandonment should galvanize the resolve to understand the highest priority sites, and where possible remediate them.

Commissioning of the ALICE readout software for LHC Run 3

ALICE (A Large Ion Collider Experiment) is a heavy-ion detector studying the physics of strongly interacting matter and the quarkgluon plasma at the CERN LHC (Large Hadron Collider). During the second long shut-down of the LHC, the ALICE detector was upgraded to cope with an interaction rate of 50 kHz in Pb-Pb collisions, producing in the online computing system (O2) a sustained input throughput of 3.5 TB/s. In the past years, the O2/FLP project built the new data-acquisition system capable of handling this load. It consists of 200 readout nodes, collecting the data transferred from over 8000 detector links to PCs memory by dedicated PCI boards. The readout software manages the hardware and software memory buffers used for DMA and inter-process communication. It initiates the data flow, performs on-the-fly consistency checks, formats the data, reports performance, and finally pushes the data to the local processing pipeline. The output is then sent by the data distribution software over 100Gb/s links to a dedicated event processing farm. The readout software modular design allowed to address the manifold needs faced during the prototyping, installation and commissioning phases, which proved essential from the lab tests to physics production, like file replay and recording, or online multi-threaded LZ4 compression. We will describe the hardware and software implementation of the O2 readout system, and review the challenges met during the commissioning and first months of operation with LHC collisions in 2022.

Soil properties influence the toxicity and availability of Zn from ZnO nanoparticles to earthworms

To develop models that support site-specific risk assessment for nanoparticles (NPs), a better understanding of how NP transformation processes, bioavailability and toxicity are influenced by soil properties is needed. In this study, the influence of differing soil properties on the bioavailability and toxicity of zinc oxide (ZnO) NPs and ionic Zn to the earthworm Eisenia fetida was investigated. Earthworms were exposed to ZnO_NPs and ionic Zn, between 100 and 4400 mg Zn/kg, in four different natural soils (organic matter content: 1.8–16.7%, soil pH: 5.4–8.3, representing sandy loam to calcareous soils). Survival and reproduction were assessed after 28 and 56 days, respectively. Zn concentrations in soil pore waters were measured while labile concentrations of Zn were measured using an in-situ dynamic speciation technique (diffusive gradient in thin films, DGT). Earthworm Zn tissue concentrations were also measured. Soil properties influenced earthworm reproduction between soil controls, with highest reproductive output in soils with pH values of 6–7. Toxicity was also influenced by soil properties, with EC50s based on total Zn in soil ranging from 694 to >2200 mg Zn/kg for ZnO_NP and 277–734 mg Zn/kg for ionic Zn. Soil pore water and DGT measurements showed good agreement in the relative amount of Zn extracted across the four soils. Earthworms exposed to ZnO_NPs survived higher Zn concentrations in the soils and had higher tissue concentrations compared with ionic Zn exposures, particularly in the high organic content calcareous soil. These higher tissue concentrations in ZnO_NP exposed earthworm could have consequences for the persistence and trophic mobility of Zn in terrestrial systems and need to be further investigated to elucidate if there any longer-term risks associated with sustained input of ZnO_NP to soil.

EXCURSIONS INTO HEALTHCARE SYSTEMS TO HELP INFORMAL CAREGIVERS MANAGE DEMENTIA

Abstract With the establishment in 2019 of the National Institute on Aging-funded IMPACT Collaboratory, the era of designing and implementing pragmatic clinical trials in partnership with health care systems to improve care and health-related outcomes for people living with dementia (PLWD) is well underway. An important focus for Collaboratory-funded work involves targeting and engaging family and other informal caregivers of PLWD across health care system settings. In this Symposium, we feature three Collaboratory-funded investigators who are partnering with health care systems to determine how to engage informal caregivers most pragmatically, with the goals of improving caregivers’ capacity to manage dementia and provide sustained input into the clinical care of PLWD. Symposium presenters Quincy Samus and Hillary Lum are the first two Collaboratory-funded Health Care System Scholars and Richard Fortinsky is a pilot study awardee. Dr. Samus will present on her experiences and lessons learned in her multiple efforts to adapt and embed the MIND at Home family-focused care management model into health care systems, including her current work with a large managed care organization. Dr. Lum will explain ongoing capacity-building stakeholder engagement activities at a large academic health system in preparation for pragmatic clinical trials for PLWD and their caregivers. Dr. Fortinsky will present on experiences and lessons learned from ongoing efforts to identify caregivers of PLWD to join caregiver support programs, and store caregiver data electronically, in two health system outpatient care settings. Dr. Christopher Callahan, Leader of the Training Core for the Collaboratory, will serve as Symposium Discussant.

Development of Alcohol Control Policy in Vietnam: Transnational Corporate Interests at the Policy Table, Global Public Health Largely Absent.

This paper analyses input from global interests in the policy process leading up to the passing of alcohol control legislation in Vietnam in 2019. The global alcohol industry now relies on growth in volume in emerging markets in middle-income countries such as Vietnam, a large, rapidly industrialising country with a youthful population and emerging middle class. The industry's role in the alcohol policy process is compared with that of global health interests. Document analysis of letters and English language media coverage was supplemented by and triangulated with data from key informants on changes in the content of draft alcohol legislation and participant observation. The alcohol legislation was negotiated in the context of active engagement from the global alcohol industry and some input from global public health interests. The global alcohol industry established a partnership relationship with politicians using CSR and funded a local employee in Hanoi over the decade prior to the draft legislation being considered. Direct lobbying took place over the content of the legislation, which went through six published drafts. Trade and investment agreements provided a supportive environment and were referred to by both politicians and industry. In contrast public health resource was limited and lacked the support of a normative global policy to counter the economic imperatives. Vietnamese Ministry of Health proposals for cost effective alcohol policy were not enacted. Global commercial interests employed their considerable resources to engage in corporate social responsibility (CSR) and build partnerships with policy-makers over a long period, contributing significantly to an environment unsupportive of enacting effective alcohol control policy. The absence of structural support from a global health treaty on alcohol and lack of an equivalent level of long-term sustained input from global health actors contributed to the legislative outcome, which excluded proposed cost-effective policies to reduce alcohol harm.

5‐HT2 receptor antagonism reduces human motoneuron output to antidromic activation but not to stimulation of corticospinal axons

The intrinsic electrical properties of motoneurons strongly affect motoneuron excitability to fast‐acting excitatory ionotropic inputs. Serotonin (5‐HT) is a neurochemical that alters the intrinsic properties of motoneurons, whereby animal models and in vitro experiments indicate that 5‐HT increases motoneuron excitability by activating 5‐HT2 receptors on the somato‐dendritic compartment. In the current study, we examined how antagonism of the 5‐HT2 receptor affects motoneuron excitability in humans. We hypothesised that motoneuron excitability would be reduced. The 5‐HT2 antagonist cyproheptadine was administered to 10 healthy participants in a double‐blinded, placebo‐controlled, crossover trial. Electrical cervicomedullary stimulation was used to deliver a synchronised excitatory volley to motoneurons to elicit cervicomedullary motor evoked potentials (CMEPs) in the surface electromyography (EMG) signal of the resting biceps brachii. Likewise, electrical peripheral nerve stimulation was used to generate antidromic spikes in motoneurons and cause recurrent discharges, which were recorded with surface EMG as F‐waves in a resting hand muscle. Compared with placebo, we found that 5‐HT2 antagonism reduced the amplitude and persistence of F‐waves but did not affect CMEP amplitude. 5‐HT2 antagonism also reduced maximal contraction strength. The reduced recurrent discharge of motoneurons with 5‐HT2 antagonism suggests that 5‐HT2 receptors modulate the electrical properties of the initial segment or soma to promote excitability. Conversely, as cyproheptadine did not affect motoneuron excitability to brief synaptic input, but affected maximal contractions requiring sustained input, it seems likely that the 5‐HT2‐mediated amplification of synaptic input at motoneuron dendrites is functionally significant only when excitatory input activates persistent inward currents.

258 The Impact of Types of Clerking Document on the Compliance of Initial HAT Risk Assessment on Admission in Trauma and Orthopaedic Patients

Abstract Aim Assessment of risk of venous thromboembolism (VTE) is crucial as VTE is a significant problem in surgical patients. Trauma and orthopaedic (T&O) patients have additional risks given lower limbs and/or pelvic surgery with restricted mobility periods/ weightbearing status. Risks should be assessed as soon as possible after admission. At this point, clerking is a first point of patients’ information gathering. This study aims to examine the compliance of VTE risk assessment on admission against guideline and its relation to the types of clerking proforma. Method A QI project was undertaken using PDSA cycle analysing documents of clerking and VTE risk assessment in 44 T&O patients. Education sessions and routine reminder emails were conducted aimed at oncall doctors receiving admissions. A further 44 patients’ records were audited after 3 months. Primary outcome: VTE risk assessment completion on admission, secondary outcome: impact of types of admission pro-forma upon risk completion rate. Results Initial VTE risk assessment compliance was improved after interventions: from 77.27% to 88.63% generally and from 69% to 100% full compliance particularly in neck of femur cases. Use of structured pro-forma can increase the compliance rate of 91.3% (pre-intervention) to 100% (post-intervention) compared to other 2 freehand documents: 91% Vs 85.71% and 30% Vs 63.64%. Conclusions VTE risk assessment is improved through education sessions, and it is evident the use of structured clerking document can help to optimize the compliance rate although further sustained input is needed to achieve the targeted compliance rate.

Spike-Rate Adaptation in a Computational Model of Human-Shaped Spiral Ganglion Neurons.

The purpose of this study is to develop a biophysical model of human spiral ganglion neurons (SGNs) that includes voltage-gated hyperpolarization-activated cation (HCN) channels and low-threshold potassium voltage-gated, delayed-rectifier low-threshold potassium (KLT) channels, providing for a more complete simulation of spike-rate adaptation, a feature of most spiking neurons in which spiking activity is reduced in response to sustained stimulation. Our model incorporates features of spike-rate adaptation reported from in vivo studies, whilst also displaying similar behaviour to existing models of human SGNs, including the dependence of electrically evoked thresholds on the polarity of electrical pulses. Hypothesizing that the mode of stimulation-intracellular or extracellular-predicts features of spike-rate adaptation similar to in vivo studies, including the influence of stimulus intensity and pulse-rate, we find that the mode of stimulation alters features of spike-rate adaptation. In particular, the reduction in spiking over time with sustained input was generally greater for extracellular, compared to intracellular, stimulation, when simulating a multi-compartment SGN with human morphological features. In contrast, time-constants of spike-rate adaption reported for in vivo data did not fit our predicted responses, highlighting the need for a more complete physiological understanding of the factors contributing to spike-rate adaptation in electrically stimulated human SGNs. Our model extends previous computational models of SGNs with human morphology with ionic channels accounting for features of spike-rate adaptation. The significance of this work resides in the ability to improve the modeling of cochlear implant (CI) stimulation and its effects on neural responses. This will help develop novel, and perhaps personalised, stimulation strategies to reduce variability in CI user outcomes.

Membrane electrical properties of mouse hippocampal CA1 pyramidal neurons during strong inputs

In this work, we highlight an electrophysiological feature often observed in recordings from mouse CA1 pyramidal cells that has so far been ignored by experimentalists and modelers. It consists of a large and dynamic increase in the depolarization baseline (i.e., the minimum value of the membrane potential between successive action potentials during a sustained input) in response to strong somatic current injections. Such an increase can directly affect neurotransmitter release properties and, more generally, the efficacy of synaptic transmission. However, it cannot be explained by any currently available conductance-based computational model. Here we present a model addressing this issue, demonstrating that experimental recordings can be reproduced by assuming that an input current modifies, in a time-dependent manner, the electrical and permeability properties of the neuron membrane by shifting the ionic reversal potentials and channel kinetics. For this reason, we propose that any detailed model of ion channel kinetics for neurons exhibiting this characteristic should be adapted to correctly represent the response and the synaptic integration process during strong and sustained inputs.

MATRIX16: A 16-Channel Low-Power TDC ASIC with 8 ps Time Resolution

This paper presents a highly configurable 16-channel TDC ASIC designed in a commercial 180 nm technology with the following features: time-of-flight and time-over-threshold measurements, 8.6 ps LSB, 7.7 ps jitter, 5.6 ps linearity error, up to 5 MHz of sustained input rate per channel, 9.1 mW of power consumption per channel, and an area of 4.57 mm2. The main contributions of this work are the novel design of the clock interpolation circuitry based on a resistive interpolation mesh circuit and the capability to operate at different supply voltages and operating frequencies, thus providing a compromise between TDC resolution and power consumption.

Dynamics of the Auditory Continuity Illusion.

Illusions give intriguing insights into perceptual and neural dynamics. In the auditory continuity illusion, two brief tones separated by a silent gap may be heard as one continuous tone if a noise burst with appropriate characteristics fills the gap. This illusion probes the conditions under which listeners link related sounds across time and maintain perceptual continuity in the face of sudden changes in sound mixtures. Conceptual explanations of this illusion have been proposed, but its neural basis is still being investigated. In this work we provide a dynamical systems framework, grounded in principles of neural dynamics, to explain the continuity illusion. We construct an idealized firing rate model of a neural population and analyze the conditions under which firing rate responses persist during the interruption between the two tones. First, we show that sustained inputs and hysteresis dynamics (a mismatch between tone levels needed to activate and inactivate the population) can produce continuous responses. Second, we show that transient inputs and bistable dynamics (coexistence of two stable firing rate levels) can also produce continuous responses. Finally, we combine these input types together to obtain neural dynamics consistent with two requirements for the continuity illusion as articulated in a well-known theory of auditory scene analysis: responses persist through the noise-filled gap if noise provides sufficient evidence that the tone continues and if there is no evidence of discontinuities between the tones and noise. By grounding these notions in a quantitative model that incorporates elements of neural circuits (recurrent excitation, and mutual inhibition, specifically), we identify plausible mechanisms for the continuity illusion. Our findings can help guide future studies of neural correlates of this illusion and inform development of more biophysically-based models of the auditory continuity illusion.

Effects of effluents from wastewater treatment plants on the abiotic and biotic uptake of phosphorus by streambed sediments

Sustained inputs of nitrogen and/or phosphorus from wastewater treatment plants (WWTPs) to aquatic environments lead to an increased risk of eutrophication. The purpose of this study was to analyze the relative proportions of biotic and abiotic processes in the uptake of phosphorus by streambed sediments and their response to exogenous carbon (sodium acetate) concentration gradients, as well as explore the effects of WWTP effluent discharges on the biotic and abiotic uptake potential of phosphorus by streambed sediments in the receiving streams. Experiments were undertaken with streambed sediments collected from four different sites in different seasons, with or without the addition of a chloroform solution (biological inhibitor), to which were added different concentrations of a source of carbon (sodium acetate). After completing the indoor simulation culture, the total uptake potential (SPUlive), abiotic uptake potential (SPUabiotic), and biotic uptake potential (SPUbiotic) of phosphorus by the sediments were calculated. The SPUlive values of the sediments in the receiving section of the WWTP effluent were higher than those at the control site and gradually decreased with the flow direction. A larger amount (c. 79.04–98.11%) of the added phosphorus was taken up abiotically by the sediments with no added carbon. The SPUbiotic value of all sampling sites increased with the increase in exogenous carbon concentration by the addition of acetate, and accordingly the maximum contribution rate of the biotic uptake of phosphorus in sediments was 14.83–36.40%. Although WWTP effluents can increase the risk of release of phosphorus from sediments to the overlying water, the abiotic and biotic uptake potential of phosphorus by sediments is significantly increased by sediment–nutrient interactions. There may be more serious carbon limitations in the uptake of phosphorus by streambed sediments downstream of WWTPs than those at the reference site.

The largest negative carbon isotope excursions in Neoproterozoic carbonates caused by recycled carbonatite volcanic ash

The late Ediacaran Shuram Excursion (SE) records the most prominent negative δ13C excursions (δ13C = −12‰) during Earth’s history. It has been hypothesized to have resulted from oxidation of dissolved organic matter, diagenetic or authigenic precipitates. However, the origin of the SE remains enigmatic; current models face challenges regarding the significant amount of atmospheric oxygen needed to balance such extensive oxidation and sustained inputs of light carbon with extremely negative C isotope compositions. Here, we show that the Doushantuo Formation at the Jiulongwan section in South China, a key stratum recording the SE event, contains mineralogical and geochemical signatures related to igneous processes. Both the occurrence of ankerite, feldspar, moissanite and euhedral quartz in the SE samples and the relatively consistent Ce anomalies of carbonate and O isotopes of quartz indicate a contribution from an igneous source. In particular, the SE samples have trace element and C isotope compositions similar to those of recycled carbonatites formed by decarbonation and melting of sedimentary carbonate rocks. These observations suggest that the deep cycle of ancient carbonate rocks, which were subjected to decarbonation during subduction, melting and eruption related to the breakup of the Rodinia supercontinent, contributed to the SE. This igneous model for the SE may provide a connection between the deep and shallow carbon cycles of the Earth.

Type III Responses to Transient Inputs in Hybrid Nonlinear Neuron Models

Experimental characterization of neuronal dynamics involves recording both of spontaneous activity patterns and of responses to transient and sustained inputs. While much theoretical attention has ...

Volatilization of polycyclic aromatic hydrocarbons (PAHs) over the North Pacific and adjacent Arctic Ocean: The impact of offshore oil drilling.

Air and seawater samples were collected in 2016 over the North Pacific Ocean (NPO) and adjacent Arctic Ocean (AO), and Polycyclic Aromatic Hydrocarbons (PAHs) were quantified in them. Atmospheric concentrations of ∑15 PAHs (gas+particle phase) were 0.44-7.0ngm-3 (mean=2.3ngm-3), and concentrations of aqueous ∑15 PAHs (dissolved phase) were 0.82-3.7ngL-1 (mean=1.9ngL-1). Decreasing latitudinal trends were observed for atmospheric and aqueous PAHs. Results of diagnostic ratios suggested that gaseous and aqueous PAHs were most likely to be related to the pyrogenic and petrogenic sources, respectively. Three sources, volatilization, coal and fuel oil combustion, and biomass burning, were determined by the PMF model for gaseous PAHs, with percent contributions of 10%, 44%, and 46%, respectively. The 4- ring PAHs underwent net deposition during the cruise, while some 3- ring PAHs were strongly dominated by net volatilization, even in the high latitude Arctic region. Offshore oil/gas production activities might result in the sustained input of low molecular weight 3- ring PAHs to the survey region, and further lead to the volatilization of them. Compared to the gaseous exchange fluxes, fluxes of atmospheric dry deposition and gaseous degradation were negligible. According to the extrapolated results, the gaseous exchange of semivolatile aromatic-like compounds (SALCs) may have a significant influence on the carbon cycling in the low latitude oceans, but not for the high latitude oceans.

Hyperexcitable Neurons Enable Precise and Persistent Information Encoding in the Superficial Retrosplenial Cortex

The retrosplenial cortex (RSC) is essential for memory and navigation, but the neural codes underlying these functions remain largely unknown. Here, we show that the most prominent cell type in layers 2/3 (L2/3) of the mouse granular RSC is a hyperexcitable, small pyramidal cell. These cells have a low rheobase (LR), high input resistance, lack of spike frequency adaptation, and spike widths intermediate to those of neighboring fast-spiking (FS) inhibitory neurons and regular-spiking (RS) excitatory neurons. LR cells are excitatory but rarely synapse onto neighboring neurons. Instead, L2/3 is a feedforward, not feedback, inhibition-dominated network with dense connectivity between FS cells and from FS to LR neurons. Biophysical models of LR but not RS cells precisely and continuously encode sustained input from afferent postsubicular head-direction cells. Thus, the distinct intrinsic properties of LR neurons can support both the precision and persistence necessary to encode information over multiple timescales in the RSC.

Occurrence, gas-particle partitioning, and sources of polybrominated diphenyl ethers in the atmosphere over the Yangtze River Estuary, East China Sea.

To investigate the occurrence, gas-particle partitioning, and potential sources of polybrominated diphenyl ethers (PBDEs) in the atmosphere over the Yangtze River Estuary, gas and particle samples were collected at the remote Huaniao Island, East China Sea, during a whole year from 2013 to 2014. Nine PBDEs, with total atmospheric concentration of Σ9BDEs of 20.3 ± 26.5 pg/m3, were found in both the gas and particle phases in most samples. BDE-209 dominated both the gas and particle phases, which is consistent with the PBDE usage record in China. Seasonal variation of particle-phase Σ9BDEs was observed, with the highest concentration in winter and the lowest in summer; however, a reversed seasonal trend was observed in the gas phase. Correlation analysis between log Kp and log KOA suggested that the gas-particle (G/P) partitioning was in a non-equilibrium state, particularly for BDE-209 throughout the year. The KOA-based adsorption model prediction performed relatively well for the particle-phase fraction of Br<10-BDEs, but largely overestimated BDE-209. A steady-state model could be superior to predict G/P partitioning of BDE-209 based on annual values, though with the exception of summer samples. A relatively higher gas-phase distribution for BDE-209 than high-brominated BDEs was observed, especially in summer, when it reached 73%, implying a sustained input of gas-phase BDE-209. The potential source contribution function showed that the possible source regions for BDE-209 included Shandong and Jiangsu Provinces (the main BDE-209 production regions in China), the Yangtze River Delta region, and the southeastern coastal areas (which hosts intensive electronic waste recycling activities).

Electrostatic Forces in Field-Perturbed Equilibria: Nanopore Analysis of Cage Complexes

Summary Molecular recognition has been widely investigated under equilibrium conditions, but little is known about such processes when perturbed by external forces. Here, we investigate the influence of electric fields on complexes formed between metallosupramolecular cages and a protein nanopore at the single-molecule level. Association rates were dominated by the applied voltage, whereas local electrostatic interactions between the cage and the nanopore more greatly influenced the dissociation kinetics. By exploiting these principles, we showed that the externally applied voltage could be used to selectively bind a specific cage from a mixture containing a large excess of other cages. Moreover, the applied voltage could also be used to drive supramolecular enantio-inversion of the chiral cages or, occasionally, the non-equilibrium capture and disassembly of cages deep within the nanopore. Similar principles might be exploited in the design of other molecular devices that operate within externally applied electric fields or biogenic transmembrane potentials.