Sustainable Production Research Articles

Comparative Characterization of Three Homologous Glutathione Transferases from the Weed Lolium perenne

The comparative analysis of homologous enzymes is a valuable approach for elucidating enzymes’ structure–function relationships. Glutathione transferases (GSTs, EC. 2.5.1.18) are crucial enzymes in maintaining the homeostatic stability of plant cells by performing various metabolic, regulatory, and detoxifying functions. They are promiscuous enzymes that catalyze a broad range of reactions that involve the nucleophilic attack of the activated thiolate of glutathione (GSH) to electrophilic compounds. In the present work, three highly homologous (96–98%) GSTs from ryegrass Lolium perenne (LpGSTs) were identified by in silico homology searches and their full-length cDNAs were isolated, cloned, and expressed in E. coli cells. The recombinant enzymes were purified by affinity chromatography and their substrate specificity and kinetic parameters were determined. LpGSTs belong to the tau class of the GST superfamily, and despite their high sequence homology, their substrate specificity displays remarkable differences. High catalytic activity was determined towards hydroxyperoxides and alkenals, suggesting a detoxification role towards oxidative stress metabolites. The prediction of the structure of the most active LpGST by molecular modeling allowed the identification of a non-conserved residue (Phe215) with key structural and functional roles. Site-saturation mutagenesis at position 215 and the characterization of eight mutant enzymes revealed that this site plays pleiotropic roles, affecting the affinity of the enzyme for the substrates, catalytic constant, and structural stability. The results of the work have improved our understanding of the GST family in L. perenne, a significant threat to agriculture, sustainable food production, and safety worldwide.

Can farmers’ adoption of conservation tillage achieve sustainable agricultural production targets? Evidence from the Jianghan Plain, Hubei, China

ABSTRACT As a major agricultural country, China is under threat from land degradation because of long-term high-carbon-emission land use patterns. Conservation tillage (CT) technologies are expected to restore soil fertility, transform agricultural production models, and improve sustainability. However, the impacts of farmers’ CT adoption on achieving sustainable agricultural development remain unclear. To investigate the heterogeneous ecological effects resulting from farmers’ adoption of CT technologies, this study estimates the ecological effects of CT and examines the differences in these effects across different technologies, utilizing survey data from 1,449 farmers in the rural Jianghan Plain of China. The results show that farmers’ technology perceptions, previous adoption behaviour, and collective economic conditions significantly affect their CT adoption behaviour. The agricultural eco-efficiency in the Jianghan Plain is only 0.65. After removing bias from self-selection and unobservable factors, the ecological effect of CT adoption was 8.43%. The four different CT technologies evaluated in this study were found to increase agricultural eco-effects and achieve sustainable agriculture. However, the agricultural eco-effects of different CT technologies vary, indicating heterogeneity. Finally, policies that include implementing different combinations of CT models according to local conditions, technical training, and establishing subsidies for CT adoption are highly recommended.

Toward Sustainable Utilization and Production of Tartaric Acid.

Global efforts toward establishing a circular carbon economy have guided research interests towards exploring renewable technologies that can replace environmentally harmful fossil fuel-based production routes. In this context, sugar-based bio-derived substrates have been identified as renewable molecules for future implementation in chemical industries. Tartaric acid, a special C4 bio-compound with two hydroxyl and carboxylic groups in the structure, displays great potential for the food, polymer, and pharmaceutical industries due to its unique biological reactivity and performance-enhancing properties. To this point, there has yet to be a comprehensive literature review and perspective on the applications and synthesis of tartaric acid. As such, we have conducted a detailed and thorough outlook and discussion in terms of biological activity, organic synthesis, catalysis, structural characterization and synthetic routes. Lastly, we provide a critical discussion on the applications and synthesis of tartaric acid to give our insights into developing sustainable chemical technologies for the future.

Deciphering Electrocatalytic Hydrogen Production in Water Through a Bioinspired Water-Stable Copper(II) Complex Adorned with (N2S2)-Donor Sites.

Electrocatalytic hydrogen production stands as a pivotal cornerstone in ushering the revolutionary era of the hydrogen economy. With a keen focus on emulating the significance of hydrogenase-like active sites in sustainable H2 generation, a meticulously designed and water-stable copper(II) complex, [Cl-Cu-LN2S2]ClO4, featuring the N,S-type ligand, LN2S2 (2,2'-((butane-2,3-diylbis(sulfanediyl))bis(methylene))dipyridine), has been crafted and assessed for its prowess in electrocatalytic H2 production in water, leveraging acetic acid as a proton source. The molecular catalyst, adopting a square pyramidal coordination geometry, undergoes -Cl substitution by H2O during electrochemical conditions yielding [H2O-Cu-LN2S2]2+ as the true catalyst, showcases outstanding activity in electrochemical proton reduction in acidic water, achieving an impressive rate of 241.75 s-1 for hydrogen generation. Controlled potential electrolysis at -1.2 V vs. Ag/AgCl for 1.6 h reveals a high turnover number of 73.06 with a commendable Faradic efficiency of 94.2 %. A comprehensive analysis encompassing electrochemical, spectroscopic, and analytical methods reveals an insignificant degradation of the molecular catalyst. However, the post-CPE electrocatalyst, present in the solution domain, signifies the coveted stability and effective activity under the specified electrochemical conditions. The synergy of electrochemical, spectroscopic, and computational studies endorses the proton-electron coupling mediated catalytic pathways, affirming the viability of sustainable hydrogen production.

Transformational leadership for sustainable productivity in higher education institutions of Cameroon

AbstractThis study investigates the need for transformational leadership in the sustainable development of the productivity in higher education institutions (HEIs) in Cameroon. While institutions always request additional funding, the need for effective allocation and utilization of existing resources is relevant for sustainability. Participants for this study included teachers from state and private HEIs in Cameroon. Correlation and regression models were used to assess the impact of transformational leadership on sustainable productivity grounded in Michael Fullan’s (2011) six secrets of educational change theory. A positive and significant relationship was observed between transformational leadership and the productivity of HEIs. While the practice of transformational leadership was observed more in state-owned HEIs, the impact was more in private than in state HEIs. The mean value for intellectual stimulation for sustainable productivity of higher institutes of learning is higher for the private as compared to that of state institutions showing that intellectual stimulation is more used in private HEIs to improve their productivity than in state-owned HEIs in Cameroon. While the correlational analysis showed that intellectual stimulation has the strongest correlate effect on sustainable productivity, the regression analysis showed that inspirational motivation has more added value to productivity. Based on these findings, we recommend a leadership model for sustaining organizational productivity, where the essence of the effective practice of transformational leadership is collaboration, especially in the change process.

Uncovering novel polyhydroxyalkanoate biosynthesis genes and unique pathway in yeast hanseniaspora valbyensis for sustainable bioplastic production

This study delves into the exploration of polyhydroxyalkanoate (PHA) biosynthesis genes within wild-type yeast strains, spotlighting the exceptional capabilities of isolate DMG-2. Through meticulous screening, DMG-2 emerged as a standout candidate, showcasing vivid red fluorescence indicative of prolific intracellular PHA granules. Characterization via FTIR spectroscopy unveiled a diverse biopolymer composition within DMG-2, featuring distinct functional groups associated with PHA and polyphosphate. Phylogenetic analysis placed DMG-2 within the Hanseniaspora valbyensis NRRL Y-1626 group, highlighting its distinct taxonomic classification. Subsequent investigation into DMG-2’s PHA biosynthesis genes yielded promising outcomes, with successful cloning and efficient PHA accumulation confirmed in transgenic E. coli cells. Protein analysis of ORF1 revealed its involvement in sugar metabolism, supported by its cellular localization and identification of functional motifs. Genomic analysis revealed regulatory elements within ORF1, shedding light on potential splice junctions and transcriptional networks influencing PHA synthesis pathways. Spectroscopic analysis of the biopolymer extracted from transgenic E. coli DMG2-1 provided insights into its co-polymer nature, comprising segments of PHB, PHV, and polyphosphate. GC-MS analysis further elucidated the intricate molecular architecture of the polymer. In conclusion, this study represents a pioneering endeavor in exploring PHA biosynthesis genes within yeast cells, with isolate DMG-2 demonstrating remarkable potential. The findings offer valuable insights for advancing sustainable bioplastic production and hold significant implications for biotechnological applications.

Oceanic Breakthroughs: Marine-Derived Innovations in Vaccination, Therapy, and Immune Health

The vast, untapped potential of the world’s oceans is revealing groundbreaking advancements in human health and vaccination. Microalgae such as Nannochloropsis spp. and Dunaliella salina are emerging as resources for recombinant vaccine development with specific and heterologous genetic tools used to boost production of functional recombinant antigens in Dunaliella salina and Nannochloropsis spp. to induce immunoprotection. In humans, several antigens produced in microalgae have shown potential in combating diseases caused by the human papillomavirus, human immunodeficiency virus, hepatitis B virus, influenza virus, Zika virus, Zaire Ebola virus, Plasmodium falciparum, and Staphylococcus aureus. For animals, microalgae-derived vaccine prototypes have been developed to fight against the foot-and-mouth disease virus, classical swine fever virus, vibriosis, white spot syndrome virus, and Histophilus somni. Marine organisms offer unique advantages, including the ability to express complex antigens and sustainable production. Additionally, the oceans provide an array of bioactive compounds that serve as therapeutics, potent adjuvants, delivery systems, and immunomodulatory agents. These innovations from the sea not only enhance vaccine efficacy but also contribute to broader immunological and general health. This review explores the transformative role of marine-derived substances in modern medicine, emphasizing their importance in the ongoing battle against infectious diseases.

Agricultural waste-based lactic acid production by the fungus Rhizopus oryzae: a tool for sustainable polylactic acid production for agricultural use - a review

Agricultural waste-based lactic acid production by the fungus Rhizopus oryzae: a tool for sustainable polylactic acid production for agricultural use - a review

Advancing Vegetable Grafting: A Comprehensive Review of Techniques, Challenges, and the Future of Automated Solutions

Vegetable grafting, a practice that combines two plant parts rootstock and scion into a single, superior plant, is an increasingly important technique in modern agriculture. By enhancing yields, stress resistance, and disease tolerance, grafting plays a crucial role in addressing challenges such as soil borne pathogens, extreme environmental conditions, and crop vulnerability to nematodes. Despite its benefits, vegetable grafting faces several challenges, including the need for compatible plant material, labour-intensive nursery production, high seed costs, and the potential for pathogen spread. Effective grafting requires meticulous selection of rootstock and scion, precise grafting techniques, and optimal post-grafting care to ensure survival and success. Looking toward the future, significant advancements in grafting technologies and nursery practices are required to overcome these challenges and improve efficiency. The development of grafting robots, capable of mechanizing the grafting process, holds promise for increasing productivity while reducing labor costs. Additionally, the establishment of specialized plug plantlet nurseries, equipped with advanced seeders, growth chambers, and acclimatization facilities, offers a pathway to enhance seedling vigour and uniformity in developing countries. Future research should also focus on refining seed-priming methods, improving seedling storage technologies, and exploring digital tools such as databases, crop models, and mobile applications to optimize grafting practices. These innovations could provide valuable insights for growers, helping them select the best rootstock-scion combinations and manage crops more efficiently. With continued research and technological advancements, vegetable grafting is poised to become a global solution, offering sustainable growth and productivity improvements in agriculture.

Optimized Polyhydroxybutyrate Production by Neobacillus niacini GS1 Utilizing Corn Flour, Wheat Bran, and Peptone: A Sustainable Approach

Plastic pollution is a pressing environmental challenge, necessitating the development of biodegradable alternatives like polyhydroxybutyrate (PHB). This study focuses on optimizing PHB production by Neobacillus niacini GS1, a bacterium isolated from a municipal dumping site. By utilizing agricultural residues such as corn flour, wheat bran, and peptone as substrates, we aimed to establish an eco-friendly method for biopolymer production, contributing to sustainable agricultural residue management and bioplastic innovation. The bacterium was identified using morphological, biochemical, and molecular techniques. The optimization process involved adjusting variables such as inoculum age, inoculum size, incubation time, agitation rate, incubation temperature, pH of the medium, carbon sources, and nitrogen sources. Response surface methodology (RSM) was employed to identify optimal conditions, with the highest PHB yield of 61.1% achieved under specific conditions: 37 °C, pH 7, and an agitation rate of 150 rpm. These findings underscore the potential of Neobacillus niacini GS1 in converting agro-industrial residues into valuable biopolymers, promoting sustainable bioplastic production, and advancing agricultural residue valorization efforts through the use of eco-friendly materials.

Integrated organic and mineral fertilizer strategies for achieving sustainable maize yield and soil quality in dry sub-humid inceptisols

Maize is one of the important cereal crops grown in rainfed regions of northwestern Himalayas, however, persistent use of chemical fertilizers coupled with poor soil nutrients and water holding capacity due to coarse textured soils poses serious threat to sustaining maize yield and soil health. To address these bottlenecks, a long-term experiment with application of organic manures and mineral fertilizer provides insights to quantify changes in soil organic carbon (SOC), crop yield and rain water use efficiency (RWUE) in rainfed area having low water use efficiency. A twelve years field experiment was conducted under dry sub-humid Inceptisols in northern India to study the potential impacts of organic and mineral fertilization on maize (Zea mays L.) productivity, water use efficiency and soil quality. Ten treatments were assessed, involving different nitrogen levels (20, 30, and 40 kg N ha⁻¹) combined with 10 tha⁻¹ year⁻¹ of farmyard manure (FYM), in-situ green manure from sunhemp, and the incorporation of Leucaena leucocephala leaves at 5 tha⁻¹ year⁻¹, including an unfertilized control. Maize yield increased linearly with increasing nitrogen application rates. The combination of FYM @ 10t ha−1 and 40 kg N ha−1(T4) yielded the highest maize production. Manure addition improved soil organic carbon (SOC) and major soil nutrients (N, P and K) while unfertilized control showed decline in soil nutrients compared to their initial values. Compared with control, incorporation of 10 t ha−1 FYM increased SOC by 1.3, 1.41, 1.44 times at application rate of 20, 30, 40 kg N ha−1, respectively. Application of N@40 kg ha−1 + 10t FYM ha−1 showed highest rain water use efficiency (RWUE) and relative production efficiency index (RPEI) (2.74 kg ha−1 mm−1 and 82, respectively) and the lowest rank sum of 6. Highly significant positive relationship existed between RPEI and RWUE, RPEI and sustainability yield index (SYI), RWUE and SYI indicated the superiority of FYM in combination with mineral fertilizer. Regression models, correlating yield with monthly rainfall and crop growing periods, indicated that the integration of FYM (10 tha⁻¹) with 40 kg N ha⁻¹ was most effective in achieving the highest relative soil quality index (RSQI) of 76 and the greatest sustainability yield index (SYI) of 49.3%. Based on results, we recommend balanced fertilization (N@40 kg ha−1 +10t FYM ha−1) which is easily manageable by farmers as the optimal strategy for improving soil quality and achieving sustainable maize productivity in nutrient depleted Inceptisols of northern India.

Amorphous silica production from Colombian rice husk: demonstration in scaled-up process Products

Introduction: the agroindustry generates significant waste, posing environmental, health, and economic challenges. Among these, rice husk, a byproduct of the food industry, stands out due to its potential as a source of silicon. Due to its silicon content, rice husk offers a unique opportunity for sustainable energy production and the extraction of high-value products, such as amorphous silicon dioxide (SiO2). However, optimizing processes for its efficient conversion remains a challenge.Objective: the aim of this study was to optimize the nitric acid concentration for the pretreatment of Colombian rice husk in order to produce high-purity amorphous SiO2 and demonstrate the feasibility of scaling up the process.Methods: a two-stage process was developed, which involved treating rice husk with nitric acid, followed by calcination at 620 °C. The nitric acid concentration was optimized to achieve the highest SiO2 purity. Material characterization was performed using thermogravimetric analysis (TGA), X-ray diffraction (XRD), X-ray fluorescence (XRF), and nitrogen adsorption-desorption. To assess the scalability of the process, the treatment was replicated on a larger scale using the optimized acid concentration.Results: the optimized process using a nitric acid concentration of 0.2 M yielded amorphous SiO2 with a purity of 94.9% and a surface area of 298 m²/g. When scaled up, the process achieved SiO2 with a purity of 95.5%, confirming the feasibility of the methodology for industrial applications. Conclusions: the treatment of rice husk with nitric acid followed by calcination proves to be an effective and scalable approach for producing high-purity amorphous SiO2. This process not only holds potential for industrial applications but also provides a sustainable solution for valorizing agroindustrial waste, contributing to the circular economy.

Scaled-up clonal propagule production in Gracilaria dura (Rhodophyta) for sustainable feedstock production and implications for circular economy

Scaled-up clonal propagule production in Gracilaria dura (Rhodophyta) for sustainable feedstock production and implications for circular economy

Appraising the potentials of reusing plastic bottles as building blocks for housing construction at Paipe village Abuja Nigeria

Plastic bottles package a multitude of commodities consumed worldwide. Upon consumption of the commodity, the disposed plastic bottles accumulate as waste, having impacts on both the aquatic and terrestrial environment. In a bid to convert such waste to wealth, plastic bottles are creatively reused for different applications, such as pedestrian bridge boats and street furniture, amongst others. Another application of reusing plastic bottles is their serving as building blocks for housing construction. Reports and research in Nigeria confirm the proliferation of plastic bottles littering the environment, which if reused in housing construction has the potential to contribute to achieving both UN Sustainable Development Goals (SDG) 11 (making human settlements sustainable) and 12 (ensuring sustainable consumption and production). Although Nigeria is traced to being the first country in Africa to reuse plastic bottles in housing construction, not much research output exists from practitioners’ experience on the potentials of reusing plastic bottles as a sustainable construction material as practiced in countries like Vietnam, India, and the Philippines, among others. As such, this study investigates the potential factors driving the practice of reusing plastic bottles in Nigeria with a view to ascertaining the satisfaction derived from the practice for sustainable housing construction. Primary data was collected using a structured questionnaire from 41 respondents identified as having experience in using plastic bottles in construction (5 staffs of Awonto Konsult as well as 36 staffs of Brains and Hammers Construction). Data was analysed descriptively using both IBM SPSS Statistics 23 as well as MS Excel to compute the Mean Score as well as the Relative Satisfaction Index (RSI). Only 30 questionnaires were successfully retrieved and fully answered. Amongst the 10 potential factors studied driving reusing plastic bottles, results show that almost all respondents tend to be ‘satisfied’ with both ‘strength and stability’ (having a Mean Value of 4.70 and RSI of 0.94) as well as ‘durability’ (having a Mean Value of 4.50; RSI of 0.90) of buildings built with plastic bottles. These two factors recorded the highest ‘satisfaction’ ratings, leaning towards ‘very satisfied’. Regarding the factor ‘fire resistance’ of buildings built with plastic bottles (having a Mean Value of 3.40; RSI of 0.68), results reveal that 50 percent of the respondents are ‘unsure’ if it is a satisfactory factor driving reusing plastic bottles or not. The study found that the satisfaction ratings of technical and environmental factors have higher appeal to respondents compared to health and safety and also financial factors. It is recommended that Awonto Konsult and also Brains and Hammers Construction invest more in information related to the fire resistance of plastic bottles used in construction because fire outbreaks pose great threats to buildings. Equally, wider empirical research on plastic bottle wastes, if undertaken, could support the development of policies for waste management, particularly in developing countries. This research has the potential to convert waste into wealth in a bid to minimising environmental impacts of disposed plastic bottles as well as contribute to sustainable materials, particularly for rural housing. Since this study was based on a survey, experimental studies of potentials driving the reuse of plastic bottles in housing construction will reveal results that could enable more sustainable housing construction in Nigeria.

Evaluating the Influence of Nutrient-Rich Substrates on the Growth and Waste Reduction Efficiency of Black Soldier Fly Larvae

Background: The black soldier fly (Hermetia illucens) has emerged as a promising tool in sustainable waste management, owing to its larvae’s ability to efficiently convert organic waste into valuable biomass. Objective: This study investigates the impact of various substrate compositions on the growth, waste reduction efficiency, and bioconversion rate of black soldier fly (BSF) larvae (Hermetia illucens). The aim is to optimize feeding strategies to enhance the effectiveness of BSF larvae in sustainable waste management and protein production. Methods: A controlled experiment was conducted over a 20-day period, using four different substrate types: 100% sludge, 75% sludge + 25% chicken feed, 25% sludge + 75% chicken feed, and 100% chicken feed. Each treatment had three replicates with 100 larvae each. Larval growth metrics, including weight and width, were recorded bi-daily. The waste reduction efficiency and bioconversion rate were calculated based on the remaining substrate weight and larval biomass, respectively. Elemental analysis was performed to determine the impact of substrate type on the accumulation of various elements in the larvae. Results: Significant differences were observed in larval growth, waste reduction efficiency, and bioconversion rates across the different substrates. The 100% chicken feed substrate led to the highest larval growth (M = 0.0881 g/day, SD = 0.0042) and bioconversion rate (M = 7.52%, SD = 0.34), while the 100% sludge substrate achieved the highest waste reduction rate (M = 86.2%, SD = 2.15). ANOVA tests indicated that substrate composition significantly affected these outcomes (p < 0.05). Elemental analysis showed substantial variations in the concentrations of calcium, cadmium, and nickel among the substrates, with the 100% sludge substrate having the highest nickel accumulation (M = 0.2763 ppm, SD = 0.023), significantly different from the other treatments (p < 0.001). Conclusions: The results demonstrate that substrate composition is crucial for optimizing BSF larvae growth and waste reduction efficiency. Nutrient-rich substrates, such as chicken feed, significantly enhance bioconversion rates and larval biomass production, although careful consideration of elemental accumulation, especially heavy metals, is essential for safe application in animal feed.

Editorial: Sustainable aquaculture production for improved food security

Editorial: Sustainable aquaculture production for improved food security

IMPACT OF QUALITY CERTIFICATION ON THE VISIBILITY AND COMPETITIVENESS OF COFFEE PRODUCTION IN CERRADO MINEIRO

The demand for sustainable agricultural practices and products that meet ethical criteria has grown among consumers, making certifications such as Fair Trade, Rainforest Alliance and UTZ essential for producers seeking to stand out in the market. This study is justified by the need to understand how certifications can add value to coffee and influence the profitability of producers, in addition to promoting practices that guarantee the economic and social sustainability of rural communities. The general objective was to investigate the impact of certifications on the visibility and competitiveness of coffee from the Cerrado Mineiro region. The methodology adopted was qualitative, with documentary analysis and literature review, following ethical guidelines and without direct interaction with producers. Data were collected from reports, academic articles and statistics from relevant institutions. The results indicated that certified coffees tend to achieve prices up to 30% higher than non-certified coffees, reflecting the willingness of consumers to invest in products that meet quality and sustainability standards. In addition, certifications encouraged the adoption of more responsible agricultural practices, generating social and environmental benefits for producing communities. The study concludes that quality certifications are essential for the competitiveness of coffee farming in Cerrado Mineiro, recommending continued support for these practices to promote a sustainable and ethical production model.

SUMOylation of rice DELLA SLR1 modulates transcriptional responses and improves yield under salt stress

Main conclusionSUMOylation of SLR1 at K2 protects productivity under salt stress, possibly by modulation of SLR1 interactome.DELLA proteins modulate GA signaling and are major regulators of plant plasticity to endure stress. DELLAs are mostly regulated at the post-translational level, and their activity relies on the interaction with upstream regulators and transcription factors (TFs). SUMOylation is a post-translational modification (PTM) capable of changing protein interaction and has been found to influence DELLA activity in Arabidopsis. We determined that SUMOylation of the single rice DELLA, SLENDER RICE1 (SLR1), occurs in a lysine residue different from the one identified in Arabidopsis REPRESSOR OF GA (RGA). Artificially increasing the SUMOylated SLR1 levels attenuated the penalty of salt stress on rice yield. Gene expression analysis revealed that the overexpression of SUMOylated SLR1 can regulate GA biosynthesis, which could partially explain the sustained productivity upon salt stress imposition. Furthermore, SLR1 SUMOylation blocked the interaction with the growth regulator YAB4, which may fine-tune GA20ox2 expression. We also identified novel SLR1 interactors: bZIP23, bHLH089, bHLH094, and OSH1. All those interactions were impaired in the presence of SUMOylated SLR1. Mechanistically, we propose that SUMOylation of SLR1 disrupts its interaction with several transcription factors implicated in GA-dependent growth and ABA-dependent salinity tolerance to modulate downstream gene expression. We found that SLR1 SUMOylation represents a novel mechanism modulating DELLA activity, which attenuates the impact of stress on plant performance.

Mammalian cell-based production of glycans, glycopeptides and glycomodules

Access to defined glycans and glycoconjugates is pivotal for discovery, dissection, and harnessing of a range of biological functions orchestrated by cellular glycosylation processes and the glycome. We previously employed genetic glycoengineering by nuclease-based gene editing to develop sustainable production of designer glycoprotein therapeutics and cell-based glycan arrays that display glycans in their natural context at the cell surface. However, access to human glycans in formats and quantities that allow structural studies of molecular interactions and use of glycans in biomedical applications currently rely on chemical and chemoenzymatic syntheses associated with considerable labor, waste, and costs. Here, we develop a sustainable and scalable method for production of glycans in glycoengineered mammalian cells by employing secreted Glycocarriers with repeat glycosylation acceptor sequence motifs for different glycans. The Glycocarrier technology provides a flexible production platform for glycans in different formats, including oligosaccharides, glycopeptides, and multimeric glycomodules, and offers wide opportunities for use in bioassays and biomedical applications.

A Paris aligned 1.5 °C mitigation pathway for the chemical industry based on 100% renewable energy and novel production technologies

With renewables growing at an unprecedented pace and critical carbon budget deadlines approaching, research is shifting to the new frontier of Paris aligned 1.5 °C mitigation pathways for hard-to-abate sectors, including the chemical industry sector. This is a significant challenge with chemical products such as plastics, fertilizers and many more products intertwined with today’s society and with CO2 emissions originating both from energy and from chemical conversions. This paper presents a detailed bottom-up production-based energy and emission calculation to make a 1.5 °C compatible scenario for the chemical industry based on three main measures compared to a business-as-usual scenario. This research found that the holistic combination of demand and supply measures is critical to reduce the different types of emissions in the sector (energy and non-energy process emissions). An overall reduction of 82% of CO2 emissions in 2050 is calculated with a decrease in base chemical production growth (− 21%), 100% renewable energy for heat and electricity (− 50%) and the introduction of novel electrical-based and biomass-based production technologies for ethylene, propylene, methanol and ammonia (− 10%). Critical system developments include slowing chemical demand growth with recycling and circularity best practices, advanced electrification of heat supply and the substantial market penetration of the current most advanced sustainable production technologies for methanol, ammonia, and ethylene based on their reasonable technology maturation trajectories. Here, green methanol and ammonia will require 381 TWh in 2030 and 3232 TWh in 2050 for green hydrogen electrolysis, which will represent 1% and 4% of global electricity demand.