ABSTRACT Weed management in upland rice under subtropical climate with high rainfall is challenging. The diverse weed flora in upland rice ecosystem emerges in several flushes, necessitating sequential application of herbicides. A 4-year study conducted in Meghalaya (950 m above sea level), India indicated that sequential application of Cyhalofop butyl [2-{4-(4-cyano-2-fluorophenoxy) phenoxy} propionic acid, butyl ester (R)], a grass weed killer high efficacy low volume herbicide applied 80 g ha−1 at 25 days after sowing (DAS) and 2,4-D, a broadleaf weed killer herbicide applied 0.75 kg ha−1 at 35 DAS was effective for weed control and produced significantly higher grain yield (3572 kg ha−1) of rice with the highest weed control efficiencies than other treatments. Application of Pretilachlor followed by (fb) 2,4-D or Fenoxaprop-p-ethyl fb 2,4-D yielded significantly higher chlrophyll (chl) a, chl b and total leaf chl content compared with other herbicides at 90 DAS. The highest net energy (111,443 MJ ha−1), energy profitability and benefit:cost ratio was recorded with sequential application of Cyhalofop butyl and 2,4-D. Thus, sequential application of Cyhalofop butyl and 2,4-D could provide a sustainable weed management option in upland rice under high rainfall conditions in the Eastern Himalayas, India.