Sustainable Industrial Development Research Articles

Exploration of the advantages of targeted isolation of deep-sea microorganisms and genetically engineered strains.

Oil, mineral processing and environmental restoration can be dangerous processes. Attempts are often made to apply microorganisms to reduce the risks, but the adaptability of terrestrial organisms is often weak. Although genetically engineered strains can improve their environmental adaptability through targeted modification, there are problems such as metabolite accumulation, poor plasmid stability and potential pathogenicity. Screening of extremophiles from the natural environment has become an inevitable choice. The special environment in the deep sea (high pressure, low temperature, low nutrition, high salinity) is a natural place for extremophiles to grow and survive, thus screening of extremophiles from the deep sea is conducive to the green and sustainable development of industry. In this paper, the application status and problems of genetically engineered strains are reviewed based on the microorganisms needed for extreme industry. This paper focuses on the application status and advantages of deep-sea microorganisms. It is found that their advantages are strong adaptability, stable gene, friendly environment, simple and convenient technology (compared with genetic engineering), which has a broad industry processes application prospect. This review broadens the scope of microbial applications.

Bio-Based Polyurethane Composites with Adjustable Fluorescence and Ultraviolet Shielding for Anti-Counterfeiting and Ultraviolet Protection.

Polyurethane and its composites play an important role in innovative packing materials including anticounterfeiting and ultraviolet protection, however, they are mainly derived from petroleum resources that are not sustainable. In this study, a 100% biobased thermoplastic polyurethane (Bio-TPU) was synthesized using biobased poly(trimethylene ether) glycol, pentamethylene disocyanate, and 1,4-butanediol. Subsequently, biobased tannic acid (TA) was employed to prepare biobased composites. The structures and properties of Bio-TPU and its composites were systematically evaluated. The results showed that the Bio-TPU/TA composite films had excellent and controllable fluorescence and UV-shielding properties. The fluorescence colors of the Bio-TPU/TA composite films could be adjusted to blue, green, and yellow by varying the TA content and adding coupling agents. Moreover, the UV transmittance of the Bio-TPU/TA composites decreased from 79.25 to 5.43% below 400 nm with an increasing TA content, indicating an excellent ultraviolet-barrier performance. Consequently, biobased TPU/TA composite films can be utilized as innovative anticounterfeiting materials and UV-shielding protection films. This study is expected to facilitate sustainable development in the polyurethane industry and broaden the high-end applications of polyurethane such as fashion, electronics, food manufacturing, pharmaceuticals, and finance.

A novel blockchain-based system for improving information integrity in building projects from the perspective of building energy performance

Global population growth has long been intertwined with environmental concerns and sustainable development. The building sector, a significant energy consumer worldwide, contributes substantially to total energy consumption and greenhouse gas emissions. With the projected global population projected to reach 9.7 billion by 2050, building energy consumption is expected to rise continuously, emphasizing the need to optimize energy performance for environmental sustainability. Despite efforts by many countries to formulate energy conservation objectives and strategies, empirical evidence reveals a substantial disparity between designed and actual building energy consumption, known as the building energy performance gap (BEPG). This gap poses a significant challenge to energy conservation efforts, and the lack of information integrity is a significant contributor to this gap. To address the challenge of inadequate information integrity in building energy projects, this research proposes an innovative solution based on blockchain technology. By utilizing blockchain's decentralized, transparent, and tamper-resistant nature, the proposed model aims to enhance information transmission mechanisms among stakeholders. Ten key energy-related stakeholders and various information flows within building projects are identified. Based on this, an exploratory architectural information management model incorporating blockchain technology is developed. The model features functionalities such as data recording, retrieval, transmission, and incentive mechanisms to ensure data immutability and reliable access security. Through a case study, the model's performance is evaluated in terms of storage cost, latency, and privacy, demonstrating significant time savings in uploading and transferring files. The proposed blockchain-based model offers a feasible solution to the challenges of inadequate information integrity in building energy projects, promoting collaboration and accurate information transmission. This model contributes to improving project outcomes and advancing sustainable development in the construction industry.

Research on the Efficiency of Major Airports in China’s Six Major Airport Cluster: Based on Common Frontier Super-Efficiency DEA and Malmquist Method

Airport clusters are of great significance to the sustainable development of the civil aviation transportation industry. The study utilises common frontier and super-efficiency DEA methods to assess the efficiency of China’s six major airport groups. It then employs the Malmquist index method to analyse changes in airport productivity. The results highlight regional disparities in airport efficiency. The East China Airport Group and the Southwest Airport Group consistently demonstrate excellent efficiency values, while the North China Airport Group and the Northeast Airport Group have significant room for improvement. Most airports within the groups operate at low and ineffective levels, with efficiency initially increasing and then decreasing. Moreover, the technology gap ratio (TGR) for each airport group somewhat shows a downward trend. The Malmquist index indicates that the overall factor productivity of each airport has generally remained stable, with efficiency growth primarily dependent on scale efficiency. On average, technical efficiency has increased by 1.5%. However, in terms of technological changes, most airports have experienced technological regression, indicating insufficient focus on technological improvement. Therefore, it is crucial to prioritise technological innovation and enhance management efficiency to achieve efficiency improvements in airport clusters. It is necessary to formulate strategies accurately based on the specific conditions of different regions, promote coordinated development, foster regional exchanges and cooperation, address regional disparities, ensure sustainable development of China’s airport clusters, and establish a world-class airport cluster.

Evolutionary Game Analysis of Electric Vehicle Distribution Entities with Shared Charging Facilities

This study investigates the evolutionary game dynamics among electric vehicle distribution entities in the context of shared charging facilities, addressing the critical issue of inadequate charging resources. To understand the behavior of different stakeholders under government incentive policies, we develop an evolutionary game model involving a government department and two logistics enterprises (A and B). Through stability analysis, we explore equilibrium conditions of evolutionarily stable strategies (ESSs) for the tripartite evolutionary game. To ensure the robustness of our findings, we conduct a MATLAB simulation analysis to validate the analytical results. Our findings highlight that government subsidies, the costs incurred by logistics enterprises to share charging facilities, and the additional distribution income derived from this sharing are critical in determining whether the evolutionary game can achieve a stable equilibrium state. This research enables logistics companies to optimize the use of charging resources, lower operating costs, and enhance delivery efficiency. Additionally, government subsidy policies play a crucial role in encouraging logistics enterprises to engage in charging facility sharing, thereby fostering the sustainable development of the entire logistics industry. Based on these insights, the paper offers practical recommendations to further promote the sharing of charging facilities in electric vehicle distribution.

Enterprise green supply chain management under the dual-carbon background: A case study of Geely Automobile

With the severe challenge of global climate change and the increasing constraints of resources and environment, China has put forward the goals of “carbon peaking” and “carbon neutrality” (referred to as “dual carbon”), aiming to promote green economic transformation and high-quality development. Green supply chain management, as an important means to achieve the goal of “double carbon”, is gradually becoming a key path for the sustainable development of enterprises. this study takes Geely Automobile as an example, an in-depth analysis of its green supply chain management practice under the background of “dual carbon”, discusses its successful experience and existing problems, and puts forward suggestions for further optimization, to provide reference and reference for other Chinese enterprises, and promote the green transformation and sustainable development of the entire industry.

Legal Standards for Selenium Enriched Foods and Agricultural Products: Domestic and International Perspectives

Background/Objectives: Selenium is indispensable for human health, yet vast regions worldwide grapple with selenium-deficient soils, rendering dietary intake a critical avenue for supplementation. This narrative review aims to systematically examine and compare domestic and international regulations and standards related to selenium enrichment, providing insights to enhance regulatory frameworks and standardization within the selenium-enrichment industry. Methods: From June to September 2024, we conducted a comprehensive search of official websites belonging to international organizations (e.g., Codex Alimentarius Commission, European Union) and governmental agencies of countries such as China and the United States. Keywords, like “selenium enrichment”, “selenium standards”, and “selenium detection methods”, were employed to identify pertinent regulations, standards, and guidelines encompassing intake benchmarks, detection methodologies, product specifications, technical guidelines for production, labeling requirements, and certification management norms. Results: Our analysis reveals several challenges within the current selenium-enriched regulatory and standardization systems, including inconsistent product determination criteria and limit settings, incomplete technical guidelines for selenium-enriched agricultural production, and a lack of unified regulations for labeling selenium-enriched agricultural products. Conclusions: These findings underscore the need for harmonization of standards and enhanced regulatory oversight. To address these issues, we recommend bolstering safety risk assessments for selenium-enriched agricultural products, establishing and refining a comprehensive standard system for selenium-enriched agriculture, and intensifying quality and safety supervision. This study offers a valuable reference for policymakers and stakeholders to promote the sustainable development of the selenium-enrichment industry.

Apparel Supply Chain Management and Practice under the Perspective of Sustainable Development

Abstract: This study explores the challenges faced by the apparel industry and the means of attaining sustainable development via effective supply chain management amidst the backdrop of global economic integration and the evolving information technology landscape.Firstly, the current situation of the apparel industry is analyzed, including factors such as the increase in consumers' personalized demands, the increasingly fierce competition, the rising costs of labor and raw materials, and the growing consciousness of environmental preservation. The development status and problems of apparel supply chain management are then analyzed, including the excessively long supply chain, the lack of flexibility and adaptability in management, the opacity of information, the lack of a supplier evaluation system, and the neglect of sustainable development by enterprises. It further discusses achieving sustainable development in the apparel industry, including reducing water consumption and pollution, addressing the adverse consequences arising from the rapid turnover of fashion trends, promoting the construction of a circular supply chain, etc. Corresponding solutions are proposed. Finally, this study concludes how supply chain management and sustainable development practices can inject new vitality into apparel enterprises and achieve the industry's sustainable development goals.

Distribution of Microorganisms and Antibiotic Resistance Genes in Production Wastewater During Pumped Storage Power Station Construction

The construction period of pumped storage power stations (PSPS) generates amounts of production wastewater, which may contain pathogenic bacteria and antibiotic resistance genes (ARGs) in these bacteria, potentially posing environmental and health risks. This study used the metagenome approach to analyze the distribution of microorganisms, ARGs and their correlation with water quality indicators in wastewater collected from two typical PSPSs. Coagulation system wastewater exhibits strong alkalinity (11.88), and aggregate system wastewater has high suspended solids (SS, 8 × 104 mg/L), resulting in lower richness and diversity of bacterial communities. Serpentinimonas, a kind of alkaliphilic bacteria, had the highest relative abundance (48.58–99.7%). The ARG subtypes obtained conferred wastewater resistance to tetracycline, macrolide, fluoroquinolone and so on, but wastewater treatment has limited removal effect on ARGs. The results indicate that resistant bacteria and resistance genes can still be present and distributed under highly alkaline conditions, and the removal efficiency of ARGs by wastewater treatment in PSPS is limited. Attention should be given to the environmental and health risks posed by production wastewater, thereby providing a theoretical basis for the sustainable development of the PSPS industry.

Sustainable logistics development strategy based on SWOT and analytic hierarchy process.

Due to the implementation of sustainable green development strategy, the study uses the competitive situation method and analytical hierarchy process of logistics enterprises to conduct structural analysis and weight calculation of factor indicators for their development strategies. Afterwards, the exponential smoothing prediction method was used to weight the logistics data. Moreover, through comparative analysis of the indicator evaluation system, the average weights of the advantageous factors of logistics enterprises were found to be 0.0232, 0.1102, 0.0522, and 0.0468, respectively. The energy consumption, transportation volume, and turnover of logistics transportation increased by 68.9% and 52.3% in 2022, respectively, which had a significant impact on the sustainable development of the logistics industry. It has been demonstrated that a sustainable logistics development strategy based on the enterprise competitive situation method and the analytic hierarchy process is both feasible and superior. Furthermore, this strategy has the potential to enhance the environmental benefits and green development of the economy and society.

Mix proportion design and carbon emission assessment of high strength geopolymer concrete based on ternary solid waste

To achieve dual optimization of the mechanical properties and environmental impacts of geopolymer concrete (GPC), this study proposes a high-strength geopolymer concrete (HSGPC) without coarse aggregate. The mix proportion of HSGPC was optimized using the response surface methodology, targeting compressive strength and splitting tensile strength to determine the optimal mix. Additionally, the carbon emission impact of HSGPC was assessed and compared with ordinary Portland cement concrete, ultra-high-performance concrete, and reactive powder concrete. The results indicate that the optimal mix proportion for HSGPC includes 15% fly ash content, 10.30% silica fume content, alkali activator ratio of 2.5, and a NaOH molar concentration of 10 M. Simultaneously, the carbon emissions of HSGPC are reduced by about 30% compared to ordinary Portland cement concrete. Compared to ultra-high-performance concrete and reactive powder concrete of the same strength, the production of HSGPC respectively reduces carbon emissions by 59.87% and 68.24%. This study not only provides valuable technical support for the practical application of GPC in engineering but also holds significant implications for promoting sustainable development in the construction industry.

Deciphering the impact of MreB on the morphology and pathogenicity of the aquatic pathogen Spiroplasma eriocheiris

BackgroundSpiroplasma eriocheiris has been proved to be a pathogen causing tremor disease of Eriocheir sinensis, it is also infectious to other aquatic crustaceans, resulting in a serious threat on the sustainable development of the aquaculture industry. S. eriocheiris is a helical-shaped microbe without a cell wall, and its motility is related to the cytoskeleton protein MreB which belongs to the actin superfamily and has five MreB homologs.ResultsIn this study, we purified MreB3, MreB4 and MreB5, and successfully prepared monoclonal antibodies. After S. eriocheiris treated with actin stabilizator Phalloidin and inhibitors A22, we found that Phalloidin and A22 affect the S. eriocheiris morphology by altering MreB expression. We confirmed that the ability of S. eriocheiris to invade E. sinensis was increased after treatment with Phalloidin, including that the morphology of E. sinensis blood lymphocytes was deteriorated, blood lymphocytes viability was decreased, peroxidase activity and cell necrosis were increased. On the contrary, the pathogenicity of S. eriocheiris decreased after treatment with A22.ConclusionsOur findings suggest that the MreB protein in S. eriocheiris plays a crucial role in its morphology and pathogenicity, providing new insights into potential strategies for the prevention and control of S. eriocheiris infections.

Spatial optimization strategies for China’s hydrogen infrastructure industry chain

Promoting the development of China’s hydrogen energy industry is crucial for achieving green energy transition. However, existing research lacks systematic studies on the spatial layout of the hydrogen industry chain. This study constructed a comprehensive theoretical framework encompassing hardware infrastructure, software systems, and soft power. Using multi-source heterogeneous data, GIS analysis, and NVivo text coding methods, the current regional layout and challenges of China’s hydrogen infrastructure industry chain were systematically evaluated. The findings determined that economically developed eastern regions lead in infrastructure and soft power, while central and western regions leverage their resource and manufacturing advantages. Major challenges include regional imbalances in hardware infrastructure, uneven distribution of soft power, and misalignment between software systems and actual needs. Analysis of the “14th Five-Year Plan” of various regions elucidated deep insights into the diversity of local hydrogen energy development strategies, identifying five types of hydrogen cities: resource-advantaged, market-oriented, regionally collaborative, innovation-driven, and policy-supported. Accordingly, strategies to enhance industry chain synergy, clarify city roles, and optimize regional ecosystems were proposed. It is recommended to integrate hydrogen infrastructure with urban planning and incorporate environmental impact assessments into spatial optimization decisions. This study provides a systematic analytical framework and progressive policy recommendations for the efficient and green layout of China’s hydrogen infrastructure, offering important implications for the sustainable development of the hydrogen industry and other rapidly developing economies.

Research on the green development path of prefabricated building industry based on intelligent technology

PurposeIn order to make the construction industry develop in the direction of greening, this paper analyzes whether the application of intelligent technology in prefabricated buildings can achieve carbon emission reduction, starting from the problems of weak technology and insufficient encouragement policies in the prefabricated building industry. It also designs dynamic and adjustable incentives for the smart transformation of prefabricated buildings and makes recommendations to facilitate the transformation of assembly manufacturers into “smart factories”.Design/methodology/approachThis paper takes the intelligent technology for carbon reduction, energy efficiency and policy design in the prefabricated buildings industry as the starting point. Based on in-depth expert interviews and questionnaire survey data, a linear multiple regression model is used to establish an association network of intelligent technology in the production and transportation, construction, operation and maintenance, demolition and scrapping stages. On this basis, an evolutionary game theory is used to construct a smart transformation and carbon reduction utility game model between the government and manufacturers, and relevant suggestions for smart empowerment of green construction development technology combinations and policy settings are proposed.FindingsAn assembly manufacturing plant with smart empowerment is an important way to achieve green and sustainable development in the construction industry. Among them, BIM and IoT have made a greater impact on carbon emission reduction of prefabricated buildings in all stages of the whole life cycle. The government’s proposed energy efficiency incentives and environmental tax amount will effectively increase companies' motivation for smart transformation of prefabricated buildings. However, when the environmental tax amount is low, the government should strengthen the regulation of the industry in order to increase the speed of smart transformation of assembly manufacturers. Therefore, a reasonable setting of the environmental tax rate and energy-saving incentives and flexible adjustment of the regulatory efforts can maximize the functional utility of the government in the process of smart transformation.Research limitations/implicationsThis paper focuses on the impact of intelligent technologies on the overall carbon emissions of the industry and provides an evolutionary analysis of the strategic game between the government and assembly manufacturers, the main players in the smart transformation process of prefabricated buildings. However, smart technologies for different categories of assembly manufacturing plants and strategic options for a wider range of stakeholders have not been examined in depth.Originality/valueDifferent from existing research, this study focuses on exploring the strategic game between the government and assembly manufacturers in the smart transformation of prefabricated buildings. It provides an innovative explanation of the connection between intelligent technology and carbon emissions. The study develops an evolutionary game model for both parties, addressing the research gap on the combined effects of policy incentives and intelligent technology on carbon reduction and efficiency improvement in the prefabricated buildings industry. This research not only offers practical reference for the government in designing incentive mechanisms and establishing regulatory systems but also provides feasible practical guidance for the smart transformation and carbon reduction efforts of assembly manufacturing plants.

An integrated three-stream network model for discriminating fish feeding intensity using multi-feature analysis and deep learning.

Feed costs constitute a significant part of the expenses in the aquaculture industry. However, feeding practices in fish farming often rely on the breeder's experience, leading to feed wastage and environmental pollution. To achieve precision in feeding, it is crucial to adjust the feed according to the fish's feeding state. Existing computer vision-based methods for assessing feeding intensity are limited by their dependence on a single spatial feature and manual threshold setting for determining feeding status constraints. These models lack practicality due to their specificity to certain scenarios and objectives. To address these limitations, we propose an integrated approach that combines computer vision technology with a Convolutional Neural Net-work (CNN) to assess the feeding intensity of farmed fish. Our method incorporates temporal, spatial, and data statistical features to provide a comprehensive evaluation of feeding intensity. Using computer vision techniques, we preprocessed feeding images of pearl gentian grouper, extracting temporal features through optical flow, spatial features via binarization, and statistical features using the gray-level co-occurrence matrix. These features are input into their respective specific feature discrimination networks, and the classification results of the three networks are fused to construct a three-stream network for feeding intensity discrimination. The results of our proposed three-stream network achieved an impressive accuracy of 99.3% in distinguishing feeding intensity. The model accurately categorizes feeding states into none, weak, and strong, providing a scientific basis for intelligent fish feeding in aquaculture. This advancement holds promise for promoting sustainable industry development by minimizing feed wastage and optimizing environmental impact.

Recovery of Low-Concentration Tungsten from Acidic Solution Using D318 Macroporous Resin.

Tungsten is a crucial strategic metal that plays a significant role in various fields, such as the defense industry, fine chemicals, and the preparation of new materials. During the practice of numerous tungsten smelting processes, a large amount of acidic wastewater containing low concentrations of WO3 is generated. The adsorption method, known for its simplicity, effectiveness, and ease of operation, represents the most promising approach for tungsten recovery and is vital for the sustainable development of the tungsten industry. In this study, D318 macroporous resin was used as an adsorbent to investigate its effectiveness in adsorbing WO3 from acidic solutions. Static adsorption experiments revealed that the adsorption capacity of D318 resin for WO3 was 683 mg·g-1. Kinetic analysis indicated that the controlling step for the adsorption of WO3 from acidic solutions by D318 resin was intraparticle diffusion. Thermodynamic analysis demonstrated that the adsorption process was endothermic and could occur spontaneously. By fitting the isothermal adsorption equation, it was found that the Langmuir model was more suitable for describing the adsorption process of WO3 on D318 resin in acidic solutions. The results of dynamic adsorption experiments showed that under optimized conditions, the dynamic adsorption capacity for WO3 was 529 mg·g-1; when using NaOH as the desorbent for cyclic desorption, the desorption rate for WO3 was 98.21%. XPS and SEM-EDS testing and analysis confirmed that D318 macroporous resin exhibited excellent adsorption performance for tungsten in acidic solutions.

Utilization of natural kapok and coconut fiber in thermally insulated sustainable concrete design.

Nowadays, when regenerable alternative green sources are attracting more caution under sustainability targets, kapok and coconut fibers, known as natural fibers, have come to the fore as a very significant raw material source. In this experimental study, compressive strength, thermal insulation, and pore structure characteristics of kapok fiber (KP)- and coconut fiber (CC)-incorporated concrete samples under different curing conditions were analyzed. For that purpose, randomly distributed fiber-incorporated concrete mixtures containing 0%, 0.5%, 1%, and 1.5% KP and CC fiber by the weight of cement were prepared and under H2O2 and NaClO curing conditions, the effects of KP and CC fiber inclusion on properties mentioned above of fiber-incorporated concrete samples were researched in detail. Experimental results depict that a maximum thermal conductivity coefficient decrease of 24.31% was detected at a content ratio of 1.5% by the reason of the pore modification effect of used natural fibers in the H2O2 curing group. Because of the remarkable pore modification effect of KP fiber incorporation into the cement matrix compared to the CC fiber inclusion cases, strong linear correlations revealing the insulation-strength mechanism could be detected for both H2O2 and NaClO curing cases. This work intends to promote sustainable development in the building industry by integrating natural fibers into concrete mixtures as an innovative design approach.

The Feasibility of Egypt's Photovoltaic Industry Drawing on China's Successful Experience

This study aims to evaluate the feasibility of Egypt's photovoltaic industry drawing on China's successful experience. Firstly, based on Egypt's national conditions and actual situation, the current situation, development potential, and challenges faced by the Egyptian photovoltaic market were analyzed.Egypts economic foundation remains less competitive, with outdated electricity infrastructure and long-term dependence on natural gas, posing challenges to its transformation. Egypt is one of the regions with the most concentrated solar energy resources in the world, which gives it the possibility and new opportunities to overtake in the photovoltaic field. Secondly, by analyzing the development process of China's photovoltaic industry, this paper explores China's successful experiences in policy support, market demand, technological level, financial support, and talent cultivation.Compared with China, Egypt's photovoltaic industry is still in an underdeveloped and developing stage, and requires sustained efforts in multiple aspects. This article compares the significant differences in the development of photovoltaic industries between China and Egypt, and analyzes the opportunities and challenges faced by Egypt's photovoltaic industry, as well as the corresponding strategies from three aspects: policy stability, demand activation, and desert photovoltaics. Finally, based on the research results, policy recommendations and development paths are proposed to promote the development of Egypt's photovoltaic industry, to provide reference and inspiration for the sustainable development of Egypt's photovoltaic industry.

Pathogenicity of Citrobacter freundii Causing Mass Mortalities of Macrobrachium rosenbergii and Its Induced Host Immune Response.

Citrobacter freundii is an opportunistic pathogen of freshwater aquatic animals, which severely restricts the sustainable development of the aquaculture industry. In this study, a dominant strain, named FSNM-1, was isolated from the hepatopancreas of diseased Macrobrachium rosenbergii. This strain was identified as C. freundii based on a comprehensive analysis of its morphological, physiological, and biochemical features and molecular identification. Challenge experiments were conducted to assess the pathogenicity of C. freundii to M. rosenbergii. The results showed that the FSNM-1 strain had high virulence to M. rosenbergii with a median lethal dose (LD50) of 1.1 × 106 CFU/mL. Histopathological analysis revealed that C. freundii infection caused different degrees of inflammation in the hepatopancreas, gills, and intestines of M. rosenbergii. The detection of virulence-related genes revealed that the FSNM-1 strain carried colonization factor antigen (cfa1, cfa2), ureases (ureG, ureF, ureD, ureE), and outer membrane protein (ompX), and virulence factor detection showed that the FSNM-1 strain had lecithinase, amylase, lipase, gelatinase, and hemolysin activities but did not produce protease and DNase activities. To investigate the immune response of M. rosenbergii to C. freundii, the expression levels of ALF3, MyD88, SOD, proPO, TRAF6, and TNF immune-related genes were monitored at different points of time in the hepatopancreas, gills, intestines, and hemocytes of M. rosenbergii after infection. The results demonstrated a significant upregulation in the expression levels of the ALF3, MyD88, SOD, proPO, TRAF6, and TNF genes in M. rosenbergii at the early stage of C. freundii infection. This study highlights C. freundii as a major pathogen causing mass mortality in M. rosenbergii and provides valuable insights into its virulence mechanisms and the host's immune response.

Changes of microbial active ingredients and antioxidant capacity during fermentation process of dark tea

Dark tea, a traditional fermented tea in China, is known for its unique flavor and enhanced health potential due to its fermentation process. However, previous studies on the evolution of its active ingredients and antioxidant properties have been limited by inadequate sample collection, single analytical methods, and insufficient data processing. To address these challenges, this study employed a comprehensive strategy to analyze the dynamic changes of active compounds and antioxidant efficacy during dark tea fermentation using refined sampling, diverse assay techniques, and advanced data analysis. A multi-point temporal sampling method was used to capture key stages of fermentation, ensuring comprehensive data. High-Performance Liquid Chromatography (HPLC) and various antioxidant assays (1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), Ferric ion reducing antioxidant power (FRAP)) enabled precise quantification of tea polyphenols, catechins, theaflavins, and thearubigins. Multivariate statistical analysis revealed that tea polyphenols and catechins decreased, theaflavins increased then slightly declined, and thearubigins steadily rose as fermentation progressed. These changes were linked to fluctuations in antioxidant capacity, peaking at around 30 mg/g of phenolic compounds. The study also explored optimizing fermentation to enhance the retention of beneficial components, maximizing antioxidant properties and improving product quality. This research advances the understanding of dark tea fermentation and supports the sustainable development of the dark tea industry.