Sustainable Alternative Sources Research Articles

Treated wastewater reuse and its impact on soil properties and potato and corn growth.

Water scarcity is a growing challenge in semi-arid regions. Many farmers have resorted to treated wastewater (TWW) as an available and low-cost water source. This study investigated the impact of irrigating potato (Solanum Tuberosum) and corn (Zea mays) with tertiary-treated (TW) and secondary-treated (SW) wastewater compared to freshwater, over two years. We studied the impact of TWW reuse on soil properties, soil microbes, crop yield, and potato tuber health. Irrigation of both corn and potato with TW significantly increased organic matter (OM) content; on average across both years and crops OM increased by about 35% under SW and 42% under TW. TWW irrigation also increased cation exchange capacity (CEC) by the second year under SW and TW in potato (average 67%), and by the second year under TW in corn (average 13%). TWW also enhanced soil fertility with no heavy metals contamination. However, potato field irrigated with SW showed high levels of total and thermotolerant coliforms in soil, exceeding predefined thresholds, in the second season. No microbial contamination was recorded in TW-irrigated fields, however, it raised salinity concerns compared to control with 935mg Na /kg in TW soil compared to 465mg/kg in control soil during the first season in potato soil. Significant increases in potato tillers, number of tubers (average 6 tubers/plant in TW vs 3 tubers/plant in the control), and tuber weight were recorded in season two under TW irrigation. Both SW and TW increased corn biomass during both seasons. In conclusion, TW is a sustainable alternative water source that enhances crop yields and improves soil quality. This study highlighted the critical role of TWW management and monitoring to address challenges such as salinity and microbial contamination. Further research is required to optimize TWW long-term reuse sustainable agriculture, balancing crop benefits while safeguarding human health.

Effect of multispecies swards on ruminal fermentation, methane emission and potential for climate care cattle farming- an in vitro study

Greenhouse gas (GHG) emissions from livestock ruminants, particularly methane (CH4), nitrous oxide (N2O), and indirectly ammonia (NH3) significantly contributes to climate change and global warming. Conventional monoculture swards for cattle feeding, such as perennial ryegrass or Italian ryegrass, usually require substantial fertilizer inputs. Such management elevates soil mineral nitrogen levels, resulting in GHG emissions and potential water contamination. Mitigating the environmental footprint of these farming practices requires sustainable alternative feeding strategies for cattle production. Multispecies grassland swards (grass + legumes or legumes + herbs) represent a promising alternative to monoculture grassland swards for cattle nutrition due to their reduced nitrogen requirements, excellent herbage yield, and polyphenolic compounds (tannins, formononetin, luteolin, quercetin, and acteoside) which may have positive effects on animals. This study investigated the effects of selected multispecies grassland swards (plant blends) on in vitro ruminal fermentation and DM digestibility. Three experimental blends of plants cultivated without fertilizers were utilized: 1) perennial ryegrass (PRG) + red clover (RC), 2) chicory (C) + red clover (RC), and 3) Tonic plantain (PLA) + red clover (RC). The control blend included perennial ryegrass (PRG), and red clover (RC) cultivated with fertilizer. The in vitro trial showed a reduction in CH4 production and ruminal NH3 concentration (by 14.7% and 28.8%, respectively; p<0.01) in the PLA+RC blend compared to the control. This plant blend also increased propionate concentration (p<0.05) and reduced acetate and butyrate concentrations and the acetate-propionate ratio (p<0.01). Additionally, the total protozoal and methanogen counts were mostly reduced by the PLA+RC blend (p<0.01) among all blends investigated. In conclusion, the Tonic plantain and red clover blend (PLA+RC) cultivated without fertilizers have the potential to be utilized as a sustainable alternative feed source for climate-friendly cattle production, aligning with the aims of the European Climate Care Cattle Farming project.

Breeding and biotechnology approaches to enhance the nutritional quality of rapeseed byproducts for sustainable alternative protein sources- a critical review.

Global protein consumption is increasing exponentially, which requires efficient identification of potential, healthy, and simple protein sources to fulfil the demands. The existing sources of animal proteins are high in fat and low in fiber composition, which might cause serious health risks when consumed regularly. Moreover, protein production from animal sources can negatively affect the environment, as it often requires more energy and natural resources and contributes to greenhouse gas emissions. Thus, finding alternative plant-based protein sources becomes indispensable. Rapeseed is an important oilseed crop and the world's third leading oil source. Rapeseed byproducts, such as seed cakes or meals, are considered the best alternative protein source after soybean owing to their promising protein profile (30%-60% crude protein) to supplement dietary requirements. After oil extraction, these rapeseed byproducts can be utilized as food for human consumption and animal feed. However, anti-nutritional factors (ANFs) like glucosinolates, phytic acid, tannins, and sinapines make them unsuitable for direct consumption. Techniques like microbial fermentation, advanced breeding, and genome editing can improve protein quality, reduce ANFs in rapeseed byproducts, and facilitate their usage in the food and feed industry. This review summarizes these approaches and offers the best bio-nutrition breakthroughs to develop nutrient-rich rapeseed byproducts as plant-based protein sources.

Uncovering the Potential Somatic Angiotensin-Converting Enzyme (sACE) Inhibitory Capacity of Peptides from Acheta domesticus: Insights from In Vitro Gastrointestinal Digestion.

Entomophagy is being proposed as a sustainable and nutritious alternative protein source. Additionally, insect consumption is also associated with some health benefits mediated by bioactive compounds produced during gastrointestinal (GI) digestion. The antihypertensive property resulting from the inhibition of the somatic angiotensin-converting enzyme (sACE) by small peptides is one of the most common bioactivities related to insect consumption. This study aimed to investigate the potential sACE-inhibitory capacity of six peptides (AVQPCF, CAIAW, IIIGW, QIVW, PIVCF, and DVW), previously identified by the in silico GI digestion of Acheta domesticus proteins, validate their formation after in vitro GI digestion of A. domesticus by LC-MS/MS, and assess the bioactivity of the bioaccessible digesta. The results showed that the IC50 values of AVQPCF, PIVCF, and CAIAW on sACE were 3.69 ± 0.25, 4.63 ± 0.16, and 6.55 ± 0.52 μM, respectively. The obtained digesta demonstrated a sACE-inhibitory capacity of 77.1 ± 11.8 µg protein/mL extract (IC50). This is the first report of the sACE-inhibitory capacity attributed to whole A. domesticus subjected to GI digestion without any pre-treatment or protein concentration. This evidence highlights the potential antihypertensive effect of both the digesta and the identified peptides.

Sustainable Dietary Patterns and Alternative Food Sources

Sustainable nutrition ensures optimal dietary intake by evaluating the life cycle of foods and their environmental impacts, aiming to meet current needs without jeopardizing the ability of future generations to do the same. It is characterized by low greenhouse gas emissions, minimal water footprint, efficient energy and land use, and reduced food waste. Sustainable dietary models, which have become increasingly important in the face of rising food demand and climate change challenges, include the Mediterranean Diet, Nordic Diet, DASH Diet, Double Pyramid Model, Flexitarian Diet, EAT-Lancet Commission Reference Diet, and Vegetarian and Vegan diets. Alternative protein sources such as algae, insects, and cultured meat are also significant. Algae offer sustainability through ocean use but have unclear metabolic impacts. Insects are efficient and environmentally friendly but require safety assessments. Cultured meat promises environmental benefits but faces cost and acceptance hurdles. Promoting sustainable eating habits requires a collective effort and is a shared responsibility towards our planet and ourselves.

Tea tourism landscape development through a sustainable alternative livelihood approach: a case study of Pedro tea estate, Nuwara Eliya, Sri Lanka

Tea tourism has gained traction in the tourism industry around the world as a sustainable and eco-friendly niche in agro-tourism. Sri Lanka, a significant tea-producing nation renowned for its “Ceylon Tea” brand, has the opportunity to harness tea agro-tourism as a sustainable alternative income source. This research focuses on the case of Pedro Tea Estate in Nuwara-Eliya, Sri Lanka, to examine the sustainable alternative livelihood approach in tea tourism landscape development and its impact on both the tea tourism industry and community empowerment. Tea tourism offers immersive experiences to eco-conscious travellers seeking authentic encounters with the tea production process. By integrating sustainable practices, tea tourism fosters environmental responsibility and contributes to economic development in tea-growing regions. Aligned with the Sustainable Development Goals, tea tourism demonstrates its potential to foster economic growth, social inclusion, cultural preservation, and environmental stewardship. This study aims to demonstrate how responsible practices and community engagement can enable the tea tourism industry to thrive while safeguarding the cultural and environmental heritage of tea-growing areas. The research endeavours to inspire other tea-growing regions to adopt sustainable landscape development and community empowerment strategies, fostering a more responsible and sustainable global tea tourism industry.

Valorization and optimization of Prosopis africana pod and cowpea husk wastes for densified hybrid briquette production

Biomass is an eco-friendly alternative sustainable renewable energy source when agricultural wastes are effectively utilized. It has become critical to assess the suitability of biofuel briquettes as an energy source for cooking, electricity, and fuel for heating. Certainly, the integration of energy sources into energy policies is warranted, considering the huge potential to provide energy for rural communities and the peri-urban poor with alternative fuel. This research describes a technique to generate densified hybrid solid biofuels from biomass waste and valorize them. Prosopis africana pod (PAP) and cowpea husk (CPH) wastes were combined to create high-energy-density fuel briquettes. The experiment involves improving briquette manufacturing parameters using response surface methodology to better understand the impacts of particle sizes, composition, binder concentrations, and densification pressures on briquette performance. The optimal conditions were particle size 150 µm, 50% PAP, 10% binder concentration, and densification pressure 103.42 kN/m2. The optimum values for burning rate, ignition time, specific fuel consumption, and heating value were 0.35 g/min, 2.75 min, 0.186 g/L, and 22.98 MJ/kg, respectively. The findings show the potential for using PAP and CPH biomass waste to produce environmentally friendly energy sources. This increases the potential agro-waste feedstocks for bioenergy in developing countries, reducing energy insecurity while promoting waste management, renewable energy generation, and environmental sustainability.

Extraction of cell wall pectins and hemicellulose from agro-industrial wastes: A sustainable alternative source

The efficient repurposing of agro-industrial waste has significantly enhanced the utilization of food resources. This study aims to propose a methodology for extracting cell wall polysaccharides from residues of mango (Mangifera indica), passion fruit (Passiflora edulis), and cashew (Anacardium occidentale). Polysaccharide fractions were obtained through sequential extraction protocols involving water, cyclohexane-trans-1,2-diamine tetracetate (CDTA), sodium carbonate:CDTA, and potassium hydroxide. These fractions were categorized as water-soluble pectin (WSP), CDTA-soluble pectin (CSP), sodium carbonate-soluble pectin (SSP), and hemicellulose (HC), respectively. Each polysaccharide fraction was characterized by Nuclear Magnetic Resonance (NMR) spectroscopy and Gel Permeation Chromatography (GPC). Monosaccharide composition was determined using Gas Chromatography-Mass Spectrometry (GC–MS). NMR spectra of WSP, CSP, and SSP fractions exhibited characteristic pectin features, while the HC fraction primarily comprised hemicellulose. Consequently, the proposed methodology demonstrates potential as a standardized protocol for the extraction of pectin and hemicellulose from various food sources.

Utilization of Tunnel Waste Slag for Cement-Stabilized Base Layers in Highway Engineering.

The rapid expansion of highway infrastructure in the mountainous regions of China has led to a significant increase in tunnel construction, generating substantial amounts of tunnel waste slag. Concurrently, the development of transportation infrastructure has created a critical shortage of natural aggregates, necessitating the exploration of alternative sustainable sources. This study aimed to conduct a comprehensive evaluation of the physical and mechanical properties of tunnel waste slag and explore its potential for utilization in cement-stabilized base courses for highway engineering applications. The uniaxial compressive strength of the parent rock (tunnel waste slag) ranged from 81 MPa to 89 MPa in the desiccated state, indicating its suitability for use as a construction material. This study also determined the maximum dry density (2.432 g/cm3) and optimal moisture content (5.4%) of cement-stabilized mixtures incorporating recycled aggregates derived from tunnel waste slag. The splitting tensile strength of these mixtures at 28 days varied from 0.48 MPa to 0.73 MPa, demonstrating robust mechanical performance. Moreover, the unconfined compressive strength of these mixtures escalated from 7.0 MPa at 7 days to 11.0 MPa at 90 days, signifying a substantial enhancement in strength over time. These results validate the viability of utilizing tunnel waste slag in highway engineering and furnish valuable insights for designers, concrete manufacturers, and construction firms engaged in the development of cement-stabilized aggregate base courses.

Understanding residents’ choice of urban farming systems in the Kumasi metropolis of Ghana: Land use policy implications

The heightened food insecurity in urban settings has rekindled the debate over the promotion of urban agriculture (UA) as a sustainable alternative food supply source across the globe. This study, therefore, examines the determinants of urban residents’ decisions to engage in UA and their choice of agriculture practices. Cross-sectional data was solicited from 430 urban dwellers through a multi-stage sampling technique. The data was analysed using descriptive as well as binary and multinomial logit regression models. The descriptive analysis shows that the cultivation of crops (34.7 %) predominates urban agricultural practices before crop-livestock integration (32.6 %) and livestock farming (32.6 %). The study identifies gender, age, religious affiliation, years of formal education, location of residence, household size, food insecurity status, availability of vacant land, extension access, and household income as significant determinants of urban households’ decision to participate in UA. The multinomial logit regression analysis revealed that urban farmers’ decision to practice only crop production is invariant between livestock-only production and integrated crop-livestock systems. When compared to the base category of the integrated crop-livestock system, the findings indicate that farmers’ educational background, food insecurity, engagement in salaried jobs, location, extension, and credit access are the main drivers of the decision to practice either crop-only or sole livestock production systems. The findings of this study have useful implications for the formulation of UA programs based on the different farming systems practiced in the study location.

Use of Periplaneta americana as a Soybean Meal Substitute: A Step towards Sustainable Transformative Poultry Feeds.

Insects are becoming increasingly popular as a sustainable and nutritious alternative protein source in poultry feeds, due to their high protein content, low environmental impact, and efficient feed conversion rates. Using insect-based feeds can reduce the need for traditional protein sources like soybean meal (SBM), which often contribute to environmental issues such as deforestation and high water consumption. The current experiments were devised to assess the impacts of the partial replacement of SBM with the American cockroach Periplaneta americana and black soldier fly Hermetia illucens on the performances, hematology, gut morphometry, and meat quality of male broilers (Ross 308). A total of 350, 1-day-old chicks weighing 40.05 ± 0.27 g were divided into 7 dietary treatments (5 pens/treatment and 10 birds/pen) at random, i.e., a 4, 8, or 12% SMB replacement with P. americana and H. illucens. Soybean meal was used as a basal diet and taken as a control. The results indicated that broilers fed on 12% P. americana or H. illucens showed significant improvements (p < 0.05) in feed conversion ratio, live weight, and daily weight gain. Hematological traits significantly improved (p < 0.05). A gut histology showed increased villus height, villus width, crypt depth, and villus height/crypt depth ratios, indicating improved nutrient absorption. Broiler meat fed on 12% P. americana meal had significantly higher redness and yellowness (p < 0.05). Substituting soybean meal with up to 12% P. americana or H. illucens meal in poultry feed can enhance the broilers' growth performance, hematology, gut morphometry, and meat quality. Hence, these findings imply that P. americana or H. illucens meal are viable and constructive alternative protein sources in poultry nutrition, offering a sustainable approach to meet the increasing demand for animal protein across the world.

Foods of the Future: Challenges, Opportunities, Trends, and Expectations.

Creating propositions for the near and distant future requires a design to catch the tide of the times and move with or against trends. In addition, appropriate, adaptable, flexible, and transformational projects are needed in light of changes in science, technology, social, economic, political, and demographic fields over time. Humanity is facing a period in which science and developing technologies will be even more important in solving food safety, health, and environmental problems. Adapting to and mitigating climate change; reducing pollution, waste, and biodiversity loss; and feeding a growing global population with safe food are key challenges facing the agri-food industry and the food supply chain, requiring systemic transformation in agricultural systems and sustainable future agri-food. The aim of this review is to compile scientific evidence and data, define, and create strategies for the future in terms of food security, safety, and sufficiency; future sustainable foods and alternative protein sources; factors affecting food and nutrition security and agriculture; and promising food systems such as functional foods, novel foods, synthetic biology, and 3D food printing. In this review, the safety, conservation, nutritional, sensory, welfare, and potential challenges and limitations of food systems and the opportunities to overcome them on the basis of new approaches, innovative interpretations, future possibilities, and technologies are discussed. Additionally, this review also offers suggestions for future research and food trends in light of future perspectives. This article focuses on future sustainable foods, alternative protein sources, and novel efficient food systems, highlights scientific and technological advances and new research directions, and provides a significant perspective on sustainability.

Recent Developments in the Application of Filamentous Fungus Aspergillus oryzae in Ruminant Feed.

The resource-intensive nature of the ruminant farming sector, which has been exacerbated by population growth and increasing pressure to reduce feed antibiotics and growth promoters, has sparked interest in looking for sustainable alternative feed sources to enhance ruminant production efficiency. Edible filamentous fungi, rich in macronutrients like proteins, offer promise in reducing the reliance on conventional protein sources and antimicrobials to improve feed quality and animal performance. The inclusion of single-cell proteins, particularly filamentous fungi, in ruminant feed has long been of scientific and industrial interest. This review focuses on the potential application of the extensively studied Aspergillus oryzae and its fermentation extracts in ruminant nutrition. It provides an overview of conventional ruminant feed ingredients, supplements, and efficiency. Additionally, this review analyzes the re-utilization of organic residues for A. oryzae cultivation and examines the effects of adding fungal extracts to ruminant feed on ruminal digestibility and animal performance, all within a circular bioeconomy framework.

Production of Bioactive Peptides from Microalgae and Their Biological Properties Related to Cardiovascular Disease

Microalgae are a substantial group of unicellular prokaryotic and eukaryotic marine organisms. Due to their high protein content of 50–70%, microalgae have the potential to become a sustainable alternative protein source, as well as aiding in the development of bioactive peptide-based nutraceuticals. A series of major steps are involved in the production of peptides from microalgae, which include the disruption of the microalgal cell wall, the hydrolysis of proteins, and the extraction or isolation of peptides derived from hydrolysis. Physical methods of cell wall disruptions are favored due to the ability to obtain high-quality protein fractions for peptide production. Bioactive peptides are protein fragments of two to twenty amino acid residues that have a beneficial impact on the physiological functions or conditions of human health. Strong scientific evidence exists for the in vitro antioxidant, antihypertensive, and anti-atherosclerotic properties of microalgal peptides. This review is aimed at summarizing the methods of producing microalgal peptides, and their role and mechanisms in improving cardiovascular health. The review reveals that the validation of the physiological benefits of the microalgal peptides in relation to cardiovascular disease, using human clinical trials, is required.

The Allergen Profile of Two Edible Insect Species-Acheta domesticus and Hermetia illucens.

Edible insect proteins are increasingly introduced as an alternative sustainable food source to address the world's need to feed the growing population. Tropomyosin is the main insect allergen; however, additional potential allergens are not well characterized and the impact of extraction procedures on immunological reactivity is unknown. Proteins from different commercial food products derived from cricket (Acheta domesticus) and black soldier fly (BSF) (Hermetia illucens) are extracted using five different extraction buffers. The proteins are analyzed by SDS-PAGE and immunoblotting using allergen-specific antibodies and crustacean allergic patient sera. IgE binding bands are analyzed by mass spectrometry as well as the complete allergen profile of all 30 extracts. Urea-based buffers are most efficient in extracting insect allergens. Shrimp-specific antibody cross-reactivity to tropomyosin from cricket and BSF indicates high sequence and structural similarity between shrimp and insects. Additional unique allergens are identified in both species, including hemocyanin, vitellogenin, HSP20, apolipophorin-III, and chitin-binding protein. Identifying potential allergenic proteins and their isoforms in cricket and BSF requires specific extraction approaches using urea-based methods. While tropomyosin is the most abundant and immunoreactive allergen, seven unique allergens are identified, highlighting the need for insect species-specific allergen detection in food products.

Bioactives of the Freshwater Aquatic Plants, Nelumbo nucifera and Lemna minor, for Functional Foods, Cosmetics and Pharmaceutical Applications, with Antioxidant, Anti-Inflammatory and Antithrombotic Health Promoting Properties

Despite significant progress, certain inflammation related to chronic disorders, including cardiovascular diseases (CVD) and cancer, still present high mortality rates. Thus, further study is needed to address such pathologies more appropriately. Apart from classic therapeutics, natural bioactives with less toxic side effects have gained attention, including those with potential pharmaceutical properties derived from several plants. Within this article, the potential utilization of freshwater aquatic plants as sources of bioactives with antithrombotic, anti-inflammatory and antioxidant properties is outlined. Emphasis is given to a well-established aquatic plant with known but not fully clarified and overviewed bio-functional and pharmaceutical properties, the Chinese lotus (Nelumbo nucifera), as well as to a so far neglected aquatic plant, Lemna minor, which has not yet been thoroughly reviewed for such applications. The latent usually grows naturally in large numbers at the surface of stored water basins of retrieved water from the last stages of wastewater treatment facilities. The continuous growth of this aquatic plant in such conditions further suggests that it can be a sustainable source of natural bioactives, if appropriately valorized, with an economic benefit and in a friendly environmental approach. The abundant content of both freshwater aquatic plants in bioactive components with potent antioxidant, anti-inflammatory and antithrombotic activities is thoroughly outlined, while their applications as functional ingredients in several functional products (functional foods, cosmetics and pharmaceuticals) are also discussed. The outlined outcomes urge further study of both aquatic plants and especially of Lemna spp. to fully elucidate their potential as alternative sustainable sources of bioactive ingredients for functional foods, supplements, nutraceuticals, nutricosmetics, cosmeceuticals, cosmetics and pharmaceutical products with health-promoting properties against inflammation and thrombosis related manifestations and their associated chronic disorders.

Estimating the dynamics of greenhouse gas emission during black soldier fly larvae growth under controlled environmental conditions

Edible insects are considered a sustainable alternative protein source. Several studies have indicated that greenhouse gas (GHG) emissions from insects are generally low. These data were usually recorded in closed static chambers, which did not reflect the actual rearing conditions. On the other hand, open respiration chambers need high amounts of substrate and insects, making the analyses time consuming and expensive. In the present study, a novel small-scale open gas exchange recording system for evaluation of GHG emissions from insects is presented. The device consists of four independent respiration chambers, each one equipped with a pump for supplying air, a flow meter and a septum for sampling the flowing air. A validation experiment was carried out using black soldier fly larvae as model organisms and moistened chicken feed (dry matter 40%) as rearing substrate. Three independent runs were conducted, each with two replicates, using 200 neonates larvae and 300 g of substrate. Moistened chicken feed (300 g) without larvae was used as control. Throughout the 12 days rearing period, gas sampling occurred every 12 h. Data interpolation considering a linear tendency between two successive sampling points was applied and overall amounts of gases were quantified as area under the curve. Irrespective of the run, CO2 emissions in the insect-substrate systems increased during the initial 7 d of experiment, consistently followed by a rapid decrease. Multiple cross correlation analysis showed high reproducibility (r ≥ 0.9), while significant differences in the CO2 emissions between insects-substrate system (range 7.76–11.88 g g−1 dry insects) and sole substrate (range 0.20–0.33 g g−1 initial dry substrate) were observed. Very limited N2O (≤1.13 mg g−1 dry insects) and CH4 (≤1.33 mg g−1 dry insects) emissions were detected in this experiment, indicating that the performed black soldier fly larvae rearing did not result in significant production of these GHGs. Overall, the experiment showed that the novel device may be particularly suited for quick and reliable estimation of GHG emissions from edible insects.

Dietary Pediastrum boryanum microalgal extract improves growth, enhances immunity, and regulates immune-related genes in Nile tilapia

BackgroundIdentifying alternative sustainable feed sources with high nutritional values is crucial for the future of environmentally and socially responsible aquaculture. In this regard, microalgae have been proven to have positive effects on fish health, which overwhelmed our interest in this study.MethodsPediastrum boryanum (P. boryanum) was incorporated into Nile tilapia feed at concentrations of 0, 0.75, and 1.5 mg/kg, as control, PbExt0.75, and PbExt1.5 groups to assess its effects on growth and biochemical indices, oxidant/antioxidant activities, immune and stress-related gene expression, and intestinal morphology.ResultsAfter 8 weeks, fish fed P. boryanum supplemented feed exhibited significant increases in final weight, length, condition factor, body weight gain, and specific growth rate, while the spleen-somatic index (SSI) and hepatosomatic index (HSI) showed no significant differences compared to the control group. Dietary P. boryanum supplementation also enhanced IgM levels and lysozyme activity, along with no marked effect on markers of liver function enzymes (alanine aminotransferase/ALT and aspartate aminotransferase/AST) or protein status (total protein and albumin). Furthermore, P. boryanum addition increased the activity of superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) enzymes, highlighting its antioxidant potential, whereas malondialdehyde (MDA) concentrations showed no significant differences among the groups. Gene expression analysis revealed that tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10), and transforming growth factor-β1 (TGF-β1) expression notably increased in groups fed P. boryanum containing feed, while no significant difference was observed in hepatic Heat Shock Protein 70 (HSP70) mRNA expression. Histopathological examination revealed no adverse effects of P. boryanum supplementation on the liver, spleen, or intestinal tissues. Villous height and villous surface area were notably increased in the high P. boryanum supplementation group, suggesting improved intestinal integrity and nutrient absorption.ConclusionDietary P. boryanum supplementation can potentially improve growth performance, immune response, antioxidant status, and intestinal health of Nile tilapia, making it a promising candidate for sustainable aquaculture.

Application of Carboxymethyl Cellulose and Glycerol Monostearate as Binder Agents for Protein Powder Production from Honey Bee Brood Using Foam-Mat Drying Technique.

This study investigates the development of protein powder from honey bee drone broods using foam-mat drying, a scalable method suitable for community enterprises, as well as the preservation of bee broods as a food ingredient. Initially, honey bee broods were pre-treated by boiling and steaming, with steamed bee brood (S_BB) showing the highest protein content (44.71 g/100 g dry basis). A factorial design optimized the powder formulation through the foam-mat drying process, incorporating varying concentrations of S_BB, glycerol monostearate (GMS), and carboxymethyl cellulose (CMC). The physicochemical properties of the resulting powder, including yield, color spaces, water activity, solubility, protein content, and total amino acids, were evaluated. The results showed that foam-mat drying produced a stable protein powder. The binders (CMC and GMS) increased the powder's yield and lightness but negatively affected the hue angle (yellow-brown), protein content, and amino acid content. The optimal quantities of the three variables (S_BB, GMS, and CMC) were determined to be 30 g, 6 g, and 1.5 g, or 80%, 16%, and 4%, respectively. Under this formulation, the protein powder exhibited a protein content of 19.89 g/100 g. This research highlights the potential of bee brood protein powder as a sustainable and nutritious alternative protein source, enhancing food diversification and security.

Economic feasibility and direct greenhouse gas emissions from different phosphorus recovery methods in Swedish wastewater treatment plants

Phosphorus (P) is a finite, non-renewable resource that is a critical component of fertilizers; therefore, recovering P from municipal wastewater can provide an alternative sustainable source of this nutrient. This work analyses economic impacts and greenhouse gas emissions of P recovery in Swedish municipal wastewater treatment plants. The study examines different scenarios, including P recovery technologies in individual plants and hubs, and considers various P-rich streams (supernatant, sludge, and ash) in plants, different plant sizes, and multiple sludge management strategies such as land application, incineration, and hydrochar production, under current market conditions. The goal is to identify and offer solutions tailored to local conditions, addressing both technical opportunities and strategies to reduce costs.The results show varying recovery rates: 5 % from supernatant, 36–65 % from sludge, and 17 % from sludge ash relative to total P in wastewater. Despite technical feasibility, P recovery costs are not covered at current market prices of P, indicating a lack of financial incentive, especially for smaller treatment plants. The least expensive recovery method costs about 7 k€/t P for ash, compared to 30–187 k€/t P for supernatant, however with the latter coming with the co-benefit of mitigated greenhouse gas emissions. The emissions from studied plants range from 84 to 123 kt CO2 eq (CO2 equivalent) for supernatant, 94–141 kt CO2 eq for sludge, and 75–102 kt CO2 eq for ash among different P recovery methods. Comparatively, P recovery methods from supernatant showed the lowest emissions, while the lower emissions range for ash is due to the consideration of fewer plants. Developing hub networks and converting sludge into products like hydrochar are crucial for attracting investments, enhancing P recovery, and leveraging economies of scale. Results highlight the urgency for localized strategies and proactive policy interventions to reconcile economic and environmental objectives in P recycling. Furthermore, P recovery from wastewater treatment plants, although more resource-intensive than mineral fertilizer, promotes circularity in the food chain and mitigates the risk of eutrophication.