Sustainable Agriculture Research Articles

Implementasi Pertanian Berkelanjutan melalui Pembuatan Pupuk Organik Cair di Karot, Kabupaten Manggarai

Excessive use of synthetic fertilizers causes a decrease in soil fertility, which can reduce crop production. Making liquid organic fertilizer (POC) from vegetable and fruit waste can reduce the use of synthetic fertilizers and support sustainable agriculture. The Golo Curu Permai farmer group has never implemented sustainable agriculture. This activity aims to reduce synthetic fertilizers' use to support sustainable agriculture and increase the knowledge and skills of farmer groups in producing liquid organic fertilizers independently. This activity was carried out at the Golo Curu Permai farmer group in Karot Village, Langke Rembong District, Manggarai Regency, with a total of 15 participants and through activity methods in the form of coordination and field surveys, extension, training or demonstration and evaluation. Evaluation is carried out by analyzing the initial test results before training (pre-test) and after training (post-test). The results of this activity show that the knowledge and skills of farmer groups after participating in training in making POC from organic waste increased by 30% from 50% to 85%.

Sustainable Agriculture and Climate Change: A Deep Learning Approach to Remote Sensing for Food Security Monitoring

Sustainable Agriculture and Climate Change: A Deep Learning Approach to Remote Sensing for Food Security Monitoring

OPTIMALISASI KISSIDAN ECO HILL MENUJU DESA SIDAN SEBAGAI DESA WISATA MELALUI PENGEMBANGAN SENSORY GARDEN

Desa Sidan has great potential as a sustainable tourist destination, but it requires revitalization to make its infrastructure and facilities more attractive and comfortable. Kissidan Eco Hill, as part of the ecotourism initiative in Desa Sidan, focuses on organic farming and local culture; however, optimizing management and physical revitalization are needed to enhance its appeal. The implementation of community service activities through the revitalization and optimization of Kissidan Eco Hill is designed to position Desa Sidan as a village focused on ecological education and sustainable agriculture. The main program includes educational tours of Subak, Bali's traditional irrigation system, with stages that range from planning and development to implementation, monitoring, and evaluation. The development stage focuses on improving planting media and promotion, while the implementation phase includes planting and the creation of irrigation channels. Monitoring is conducted through routine checks to assess the impact and performance of the program sustainably. Key partners, such as the Head of Sidan Village, village officials, and Kissidan Eco Hill staff, play an essential role in ensuring the smooth operation and success of this program. The optimization of Kissidan Eco Hill can be further enhanced by developing a sensory garden. The available planting area at Kissidan Eco Hill is sufficient to accommodate the planting of various plants that support the establishment of a sensory garden

Integrated Assessment and Agricultural Planning in Selenium-Rich Hilly Soils: A Case Study on Land Use, Heavy Metal Contamination, and Nutrient Element Distribution

The strategic development of selenium-enriched soil cultivation is essential for effective agricultural land management. This research explores the sustainable utilization of selenium-rich soils in Qiongzhong County, Hainan Island. An extensive evaluation was conducted on 7266 surface soil samples, assessing the selenium concentrations, nutrient levels, heavy metals, pH values, and soil organic matter (SOM). In addition, analyses of 70 samples for organochlorine compounds and PAHs were performed. The results indicated average selenium content of 0.46 mg/kg, with 55.2% of the samples surpassing the selenium enrichment threshold of 0.4 mg/kg. According to the GB15618-2018 standards, 127 samples (1.75%) showed medium or high chromium-associated risks. No contamination from organochlorine compounds or PAHs was found, including the 16 priority-controlled PAHs with an average concentration of 30.3 µg/kg, confirming the soil’s high quality. The correlation and factor analysis identified surface enrichment as the main factor influencing selenium accumulation, presenting minimal environmental risks. Consequently, three categories of selenium-rich soil were defined: selenium-rich, pollution-free, and high-nutrient selenium-rich soil. Recommendations based on the soil characteristics and existing agricultural practices were made for the cultivation of selenium-rich vegetables, rubber, and nuts. This study lays a foundation for the sustainable management of selenium-rich soils, providing insights for further research and decision-making to optimize these resources, thus promoting environmental protection and agricultural sustainability.

Advances in Soybean Genetic Improvement.

The soybean (Glycine max) is a globally important crop due to its high protein and oil content, which serves as a key resource for human and animal nutrition, as well as bioenergy production. This review assesses recent advancements in soybean genetic improvement by conducting an extensive literature analysis focusing on enhancing resistance to biotic and abiotic stresses, improving nutritional profiles, and optimizing yield. We also describe the progress in breeding techniques, including traditional approaches, marker-assisted selection, and biotechnological innovations such as genetic engineering and genome editing. The development of transgenic soybean cultivars through Agrobacterium-mediated transformation and biolistic methods aims to introduce traits such as herbicide resistance, pest tolerance, and improved oil composition. However, challenges remain, particularly with respect to genotype recalcitrance to transformation, plant regeneration, and regulatory hurdles. In addition, we examined how wild soybean germplasm and polyploidy contribute to expanding genetic diversity as well as the influence of epigenetic processes and microbiome on stress tolerance. These genetic innovations are crucial for addressing the increasing global demand for soybeans, while mitigating the effects of climate change and environmental stressors. The integration of molecular breeding strategies with sustainable agricultural practices offers a pathway for developing more resilient and productive soybean varieties, thereby contributing to global food security and agricultural sustainability.

Risk Management of Green Bean Farming Production in Gluranploso Village, Gresik District

Green beans are the most important food crop in Indonesia and production continues to increase to meet domestic demand. However, production is still insufficient due to several risks faced by farmers. This research analyzes the risk management of green bean production in Gluranploso Village using the Coefficient of Variation (KV) and Failure Mode and Effect Analysis (FMEA) methods. The results showed that yield variation was relatively low with a KV value of 17%, indicating good yield stability. The FMEA analysis identified four main sources of risk affecting production: seed aspect with an RPN value of 5.808, weather with an RPN value of 7.04, pests and diseases with an RPN value of 12.936, and technical aspects with an RPN value of 47.88, particularly delays in pest and disease control spraying. In addition, the market aspect is also a source of risk with the largest value in the increase in production input prices at 12.288. These sources require special attention in risk management. The study concludes that the implementation of appropriate risk management strategies, focusing on the identified key risk sources, can help farmers improve yield stability and farm profitability. These strategies include improving seed quality, mitigating weather risks, controlling pests and diseases, and improving technical procedures in cultivation and market risks. Thus, effective risk management can be key in increasing the productivity and sustainability of green bean farming in Gluranploso village.

The Effect of Supplier Collaboration, Transport Efficiency, Technology Adoption, and Market Demand on Distribution Efficiency and Profitability of Pumpkin Seed Supply Chain in Indonesia

This study examines the effects of supplier collaboration, transportation efficiency, technology adoption, and market demand on the distribution efficiency and profitability of the pumpkin seed supply chain in Indonesia. Using a quantitative approach, data were collected from 150 respondents involved in the supply chain, including suppliers, distributors, and logistics providers. The data were analyzed using Structural Equation Modeling-Partial Least Squares (SEM-PLS 3). The findings reveal that supplier collaboration, transportation efficiency, and technology adoption significantly enhance distribution efficiency, which in turn positively impacts profitability. Market demand was also found to moderate the relationship between distribution efficiency and profitability, particularly during periods of high demand. These findings underscore the importance of fostering collaboration, optimizing transportation, and adopting technology to improve the supply chain’s operational performance and financial outcomes. The study provides valuable insights for practitioners and policymakers aiming to enhance the agricultural supply chain's competitiveness and sustainability in Indonesia.

IMPACT OF FARMERS PERCPTION OF USING ORGANIC MANURE ON THE ONION PRODUCTION IN KUDAN LOCAL GOVERNMENT AREA OF KADUNA STATE, NIGERIA

This study assessed the impacts of farmers’ perception of using organic manure on the onion production in Kudan Local Government Area (LGA) of Kaduna State, Nigeria. The specific objectives were to: describe the socio-economic characteristics of onion farmers, assess the impacts of using organic manure on income and productivity, and perception of farmers in using organic manure. A multi-stage sampling procedure was employed to select 200 respondents for the study. Primary data were collected using structured questionnaires and analyzed through descriptive and inferential statistics. The findings revealed that the majority of respondents were male (89.5%) with a mean age of 40.76 years, and most households comprised 10-15 members. Compost manure (53%) was the most widely used organic input due to its availability and effectiveness in enhancing soil fertility. The results indicated that the use of organic manure had a positive impact on the productivity and income of onion farmers. Key socio-economic factors influencing productivity and income-included education, access to extension services where farmers received training on organic manure use, farming experience and the quantity of manure applied. For sustainability and continuous use of organic manure in Kudan LGA. Improving access to organic inputs, enhancing transportation infrastructure, and providing targeted education and training on organic farming practices are critical. These recommendations align with recent policies promoting sustainable agriculture in Nigeria.

Biochar as a strategy to manage stem rot disease of groundnut incited by Sclerotium rolfsii

Due to the pathogen’s ability to survive in the soil for longer durations, soil-borne diseases are often difficult to control. This study investigates the multifaceted impacts of biochar on the management of stem rot disease in groundnut and its influence on soil properties and microbial communities. The effects of biochar at different concentrations, such as 0%, 1%, 3%, and 5% on groundnut stem rot disease incited by Sclerotium rolfsii were evaluated thoroughly. Under laboratory conditions, biochar exhibited no direct inhibitory effects on S. rolfsii at varying concentrations but revealed an indirect suppression of sclerotial body production, suggesting a concentration-dependent influence on pathogen resting structures. Further, it was observed that biochar treatments effectively delayed symptom onset and reduced disease progression in groundnut plants, with significant variation observed among genotypes and biochar concentrations. Notably, interactions involving genotypes ICGV 171002 and ICGV 181035 with BC2 + Sr (3% conc. of biochar + S. rolfsii) and BC3 + Sr (5% conc. of biochar + S. rolfsii) treatments showed superior efficacy in disease reduction under controlled conditions. Field evaluations confirmed these findings, highlighting genotype-specific responses to biochar treatments. However, no significant difference was observed between BC2 + Sr (3%) and BC3 + Sr (5%) treatments in managing stem rot disease compared to controls. Biochar application significantly increased soil nutrient levels, including nitrogen, phosphorus, and potassium, and increased soil organic matter content, EC, pH, emphasizing its potential to improve soil fertility. Overall, these findings highlight the potential benefits of biochar for sustainable agriculture through disease management, soil nutrient enrichment, and microbial modulation, warranting further investigation into optimal application strategies across different agricultural contexts.

Effects of the Application of Organic Fertilizers on the Yield, Quality, and Soil Properties of Open-Field Chinese Cabbage (Brassica rapa spp. pekinensis) in China: A Meta-Analysis

With the development of sustainable agriculture, trials on the benefits of the application of organic fertilizers around the world have been conducted. Herein, we investigated the impact of the pure chemical fertilizers (CFs) combined with organic fertilizers compared with the application of CFs (100% CFs) and no fertilizers (NFs) on soil properties as well as the yield and quality of Chinese cabbage through meta-analysis. Results indicate that: (1) Compared with NFs, the application of organic fertilizers can significantly improve the yield and quality of Chinese cabbage and increase soil nutrients. (2) Compared with CFs, the application of organic fertilizers can increase the fresh weight, number of leaves, transverse diameter, leaf length, and development of Chinese cabbage per plant, with increases of 8.54%, 6.6%, 9.905%, 8.42%, and 10.03%; Meanwhile, organic fertilizers can significantly increase the yield (total amount of above-ground parts produced) and commercial yield (the portion that meets the required quality standards and is intended for sale) of Chinese cabbage to increase the yield and commercial yield by 10.08% and 35.56%, respectively. However, it has no significant impact on the income from growing Chinese cabbage. (3) Compared with CFs, the application of organic fertilizers can significantly increase the content of vitamin C (11.06%), soluble sugar (19.16%), and soluble protein (8.83%) and reduce the content of nitrate and nitrite in Chinese cabbage, with a reduction of up to 19.02% and 20.9%, respectively. The application of organic fertilizers will also have a certain impact on the absorption of heavy metals in Chinese cabbage. (4) Compared with CFs, the application of organic fertilizers can significantly improve soil organic matter, soil carbon sequestration, nitrogen absorption, and potassium absorption, showing increases of 12.73%, 13.19%, 7.91%, and 7.37%, and the application of organic fertilizers reduces soil electrical conductivity and available nitrogen, showing decreases of 36.78% and 38.75%, respectively. (5) The application of organic fertilizers significantly increased the content of soil urease and soil sucrase, increasing by 9.42% and 17.16%, respectively. This study helps inform the application of organic fertilizers in Chinese cabbage production.

Forecasting Western Corn Rootworm (Diabrotica virgifera virgifera LeConte) Density and Non-Chemical Control of Larvae: A Practical Review

The western corn rootworm (WCR) (Diabrotica virgifera virgifera LeConte; Chrysomelidae) is one of the most significant maize pests in Europe, with farmers spending a substantial amount (approximately 140 EUR) on its control. In the context of climate change, WCRs could pose an even greater threat to EU maize production, particularly as the European Union continues to withdraw an increasing number of effective yet environmentally harmful active agents. Biological control methods have now emerged to the forefront in creating sustainable agriculture. In this review, we carried out an extensive literature analysis on methods for forecasting WCRs and evaluated the practical applicability of the latest non-chemical control methods targeting its larvae. Effective forecasting is essential for successful pest management, enabling informed planning and the selection of the most suitable control methods. Several traditional predicting techniques remain in use today, but recent advancements have introduced modern electronic forecasting units combined with sensor-equipped pheromone and colour traps, as well as thermal sum calculations. Research has demonstrated that crop rotation is one of the most effective methods for controlling WCR larvae. Biological agents, such as entomopathogenic fungi (Beauveria bossiana and Mettarrhyzum anasoplia), entomopathogenic nematodes (Heterorhabditis bacteriophora), and botanical insecticides such as azadirachtin can significantly reduce larval populations and root damage, thereby maintaining infestation levels below the economic threshold. Genetically modified maize plants that produce specific toxins, along with conventional breeding efforts to increase root system regeneration, are also promising tools for the sustainable management of this pest. This review summarizes the solutions for prediction of western corn rootworm infestations and non-chemical control of its larvae. Accurate forecasting methods provide a clear picture of infestation levels in a given area, enabling precisely targeted control measures. In all cases, the control should be directed primarily against the larvae, thereby reducing root damage and reducing the size of the emerging imago population. This review demonstrates that biological control methods targeting larvae can be as effective as pesticides, supporting sustainable pest management.

Evaluation of herbicidal potential of Siderophores produced by Amycolatopsis lurida strain 407

The urgent need for sustainable agriculture has intensified the search for environmentally friendly alternatives to chemical herbicides. This study investigates the herbicidal potential of siderophores produced by Amycolatopsis lurida strain 407, focusing on its effects on the growth of ryegrass and redroot weeds. Strain 407 exhibited two distinct colony morphologies—red and white—when cultured under varying environmental conditions. The cell-free culture filtrate (CFCF) from both colony types significantly inhibited the growth of ryegrass and redroot. The concentration of siderophore produced in the iron-deficient medium was measured to be 613.4 ppm for 407 red and 388.5 ppm for 407 white, which indicates significant iron chelating activity. This study also showed a direct relationship between the presence of siderophore in plant culture medium and reduced growth. Also, analysis of fractions of the aqueous phase resulting from column chromatography revealed that all fractions from the 407 red reduced ryegrass shoot length by up to 45% and root length by 83–86%, while redroot seedling length decreased by up to 36%. Fractions from 407 white completely inhibited germination or reduced ryegrass root length by up to 94% and redroot seedling length by 52%. Fractions F4 W to F7 W and F2 R to F8 R, which showed iron chelating activity were most effective in reducing plant growth, suggesting that there are metabolites, alone or in company with siderophores, synergistically do herbicidal activity. The innovative application of siderophores as bioherbicide presents a promising environmentally friendly alternative to chemical herbicides.

Deciphering the role of growth regulators in enhancing plant immunity against herbivory.

Plants are central to global food production, and the pursuit of sustainability aims to enhance or preserve food quality while safeguarding the environment. Due to their immobility, plants are unable to evade unfavourable climatic setups or interactions with other living creatures. Upon their interaction with insect herbivores, plants face biotic stress, which is a constant challenge for plants, causing molecular, physiological, and biochemical changes and reducing their productivity. To combat biotic stress caused by herbivores, plants have evolved intricate defence mechanisms through growth regulators such as auxins, cytokinins, gibberellins, salicylic acid (SA), jasmonic acid (JA), ethylene (ET), abscisic acid (ABA), strigolactones and brassinosteroids. The intricate network of specific proteins, metabolites and certain phytohormones orchestrates plant defensive reactions, leading to their skilful coordination in responding to insect attacks. Comprehending the defence mechanisms holds the key to mitigating significant crop and economic losses. This review entails a comprehensive analysis of the role of growth regulators in enhancing plant immunity against herbivory, highlighting the substantial efforts by the scientific community to manage and mitigate damages from biotic stress in plants, ultimately contributing to the advancement of sustainable agriculture.

Optimization of Long Line Management in Seaweed Farming: Strategies for Increasing Farmers' Group Income in Pinrang Regency, Indonesia

This research aims to explore the role of the long-line method in increasing the income of seaweed farmers in Pinrang Regency, South Sulawesi, Indonesia, while examining the challenges they face and the strategies they employ to overcome them. The study employs a qualitative case study approach, involving 15 seaweed farmers from various coastal villages within Pinrang Regency, representing diverse demographic backgrounds and experiences. Data collection was conducted through semi-structured interviews, allowing for an in-depth understanding of the participants' farming practices and challenges. The findings indicate that seaweed farmers increase their income by optimizing resources, collaborating within farmer groups, and adapting to environmental and market changes. Strategies such as space optimization, material reuse, and knowledge sharing among farming communities significantly enhance productivity and sustainability. Furthermore, farmers employ adaptive techniques to mitigate environmental risks and market volatility, including using durable materials and processing seaweed into higher-value products. The implications of these findings are crucial for policymakers and development organizations, as they highlight the potential of sustainable farming practices in improving the livelihoods of coastal communities. Policymakers are encouraged to develop support programs that promote resource optimization, foster community-driven farming networks, and train farmers in adaptive strategies. However, the study's limitations, including the small sample size and reliance on self-reported data, may affect the generalizability of the results. Future research should incorporate larger, more diverse samples and objective measures to enhance the robustness of the findings, and explore the long-term impacts of the long-line method on income and sustainability.

Beneficial soil fungi enhance tomato crop productivity and resistance to the leaf-mining pest Tuta absoluta in agronomic conditions

Research has shown that soil-borne beneficial microorganisms can enhance plant growth, productivity, and resistance against pests and pathogens and could thus serve as a sustainable alternative to agrochemicals. To date, however, the effect of soil-beneficial microbes under commercial crop production has been little assessed. We here investigated the effect of root inoculation with nine well-characterized bacterial and fungal strains and two consortia on tomato performance under intensive tomato crop management practices. We measured the impact of these root inoculations on plant growth, fruit quality, yield, and pest and pathogen incidence. While most microbial strains showed weak effects, we found that the fungal strains Trichoderma afroharzianum T22 and Funneliformis mosseae significantly increased marketable tomato yield. Moreover, we found that inoculation with most of the fungal strains led to a significant reduction in the incidence of the devastating leaf-mining pest Tuta absoluta, while this effect was not observed for bacterial inoculants. In addition, we found that microbial inoculations did not impact the incidence of introduced natural pest enemies, supporting their compatibility with well-established integrated pest management strategies in horticulture. In summary, the observed general positive effects of soil microbes on tomato yield and resistance reinforce the move toward broader adoption of microbial inoculants in future crop production, ultimately improving agricultural sustainability.

Shaping Consumers’ Attitude and Purchase Intention towards Halal Sustainable Organic Food Products

Increased global recognition of organic food has prompted developed nations to adopt ecological agriculture concepts. This trend is also reflected within the sustainable Halal (permissible) organic food sector. This study examines whether Halal awareness, certification, social influences, and promotion affect consumers' attitude and subsequently, intention to purchase Halal organic food in Malaysia. Using convenient sampling technique, online surveys were conducted with 200 usable responses. A Smart PLS statistical software was used for data analyses. Results revealed that Halal awareness, certification, and social influence significantly affect attitudes, which, in turn, significantly influence consumers' intentions to purchase Halal organic food products. Implications are addressed.

HUBUNGAN MODAL SOSIAL PETANI TERHADAP KEBERLANJUTAN USAHATANI JAGUNG KUNING DI DESA WAKADIA KECAMATAN WATOPUTE KABUPATEN MUNA

The objective of this study is to ascertain the correlation between farmers' social capital and the sustainability of yellow corn farming in Wakadia Village, Watopute Subdistrict, Muna Regency. The study population consisted of all yellow corn farmers in Wakadia Village, Watopute Subdistrict, Muna Regency, comprising a total of 103 households. The number of samples was determined using the Slovin formula with a 10% error margin, resulting in a sample size of 51 respondents. The sampling technique employed was simple random sampling. The data collection techniques employed included surveys, interviews, and document analysis, with data collected using questionnaires. This study employs two variables: social capital, comprising trust, participation, networks, and social norms, and farm sustainability, encompassing economic, social, and environmental sustainability. The data were subjected to descriptive analysis using a class interval formula and a Rank Spearman correlation formula. The findings indicate that the social capital of farmers engaged in yellow corn farming in Wakadia Village, Watopute Subdistrict, Muna Regency is in an optimal category, suggesting that their social capital is capable of sustaining yellow corn farming over the long term. The sustainability of yellow corn farming in Wakadia Village is sufficient, indicating that farmers have implemented business sustainability indicators but still require improvement. There is a significant and positive relationship between farmers' social capital and yellow maize business sustainability in Wakadia Village.

Application of Internet of Things (IoT) on Monitoring EC and pH Values for Fertigation System

This paper introduces a novel Internet of Things (IoT) based fertigation control system designed to enhance traditional agricultural practices in Malaysia, particularly in rural areas. The system integrates a fertigation approach, combining fertilization and irrigation, with smart devices and a NodeMCU ESP32 microcontroller to automate the tracking of data from liquid fertilizer mixtures. By utilizing EC and pH sensors, the system accurately monitors the nutrient concentration and pH levels of the fertilizer mix, with data displayed on an IoT platform for real-time observation and control. This approach mitigates common issues such as overfertilization and inefficient nutrient use, offering significant improvements over manual data collection methods. The system's performance was validated, showing successful monitoring and control of the fertilization process, leading to enhanced plant health and crop quality. Key findings indicate that the system's integration with IoT technology provides a more efficient, automated solution for managing fertilizer applications, reducing labor and time while ensuring optimal plant nutrient levels. The study also highlights the potential for future development, including the integration of additional sensors and advanced data analytics to optimize fertigation practices further. The implementation of this IoT-based system represents a significant advancement towards sustainable agriculture, offering practical benefits for Malaysian farmers and setting a foundation for further innovation in fertigation technology.

Enumeration of Genetic Variability Parameters and Association among Early Seedling Growth Parameters under Polyethylene Glycol (PEG) induced Osmotic Stress in rabi Sorghum (Sorghum bicolor (l.) Moench)

Aims: The research aims to explore the variability within diverse rabi sorghum germplasms and identify the relationships among different early seedling growth traits. This will provide valuable insights for future breeding programs, aiding in the development of resilient sorghum varieties that can contribute to improved food security and agricultural sustainability. Study Design: The study was conducted by employing factorial complete randomized design (FCRD) with two replications. Where, two factors was considered, genotype as first factor and PEG concentration as second. Place and Duration of Study: The experiment was conducted during rabi season of 2023 at University of Agricultural Sciences Dharwad, Karnataka. Methodology: A total of 156 germplasm lines of rabi sorghum were assessed in vitro for early seedling growth characteristics, including germination percentage, shoot length, root length, seedling dry weight, and seedling vigor indices I and II, under both control and osmotic stress conditions induced by polyethylene glycol (PEG). Osmotic stress was induced by dissolving PEG 6000 in distilled water to achieve the desired concentration. The germplasm lines were then subjected to germination and early growth under these conditions using the paper towel method. The genetic variability parameters for each trait were quantified to examine the degree of variability in the material used, and correlation analysis was performed to understand the relationships among the traits studied. Results: The variance analysis showed highly significant differences among genotypes, treatments, and the genotype-by-environment (G×E) interactions. Genetic variability parameters revealed considerable variability across all traits studied, which tended to increase with higher stress levels. Moderate to high GCV and PCV was observed for all the traits studied. Correlation analysis demonstrated a strong, significant positive relationship among all seedling parameters, except between root length and shoot length, as well as between shoot length and germination percentage under control conditions. Conclusion: The study emphasizes the effect of osmotic stress on the early vigor and growth of sorghum seedlings. The findings reveal significant variability in seedling traits, suggesting that selection can be employed for genetic enhancement of these traits. Correlation analysis identified specific traits or combinations of traits that should be considered when selecting genotypes tolerant to osmotic stress conditions. These findings offer great potential to guide and improve selection and breeding programs focused on enhancing drought tolerant sorghum varieties.

Sustainable bioconversion of rice straw into indole-3-acetic acid by Streptomyces coelicoflavus using response surface methodology

AbstractRice straw is an abundant agricultural waste that poses environmental disposal challenges and can be utilized for biotechnological applications. This study investigates the potential of actinobacteria to enhance rice straw biodegradation and sustainable indole-3-acetic acid (IAA) production, addressing the need for sustainable agricultural practices. Certain actinobacteria strains can effectively degrade rice straw while optimizing IAA production under controlled fermentation conditions. Twenty actinobacteria isolates were screened for lignocellulolytic enzyme activity, and ten were selected for rice straw biodegradation. IAA production was further optimized using response surface methodology based on temperature, pH, and agitation speed (RPM). Isolate S16 achieved a degradation rate of 68.75%, while S18 produced the highest IAA concentration (1040.625 μg/mL) under optimized conditions (25 °C, pH 9, 160 RPM). The purified IAA significantly improved Medicago sativa L. growth. Furthermore, the 16S rRNA gene sequence of isolate S18 was identified it as Streptomyces coelicoflavus strain NSH24 with accession number PP 320383.1. These findings underscore the potential of actinobacteria to efficiently convert agricultural waste into valuable bioproducts and promote sustainable farming practices. By transforming rice straw into high-value products like IAA, this approach contributes to a circular economy, offering an environmentally friendly solution for biomass utilization and agricultural sustainability.