Susceptible Rice Genotypes Research Articles

Genome-wide analysis of calmodulin binding Protein60 candidates in the important crop plants.

Efficient management of environmental stresses is essential for sustainable crop production. Calcium (Ca²⁺) signaling plays a crucial role in regulating responses to both biotic and abiotic stresses, particularly during host-pathogen interactions. In Arabidopsis thaliana, calmodulin-binding protein 60 (CBP60) family members, such as AtCBP60g, AtCBP60a, and AtSARD1, have been well characterized for their involvement in immune regulation. However, a comprehensive understanding of CBP60 genes in major crops remains limited. In this study, we utilized the Phytozome v12.1 database to identify and analyze CBP60 genes in agriculturally important crops. Expression patterns of a Oryza sativa (rice) CBP60 gene, OsCBP60bcd-1, were assessed in resistant and susceptible rice genotypes in response to infection by the bacterial pathogen Xanthomonas oryzae. Localization of CBP60 proteins was analyzed to predict their functional roles, and computational promoter analysis was performed to identify stress-responsive cis-regulatory elements. Phylogenetic analysis revealed that most CBP60 genes in crops belong to the immune-related clade. Expression analysis showed that OsCBP60bcd-1 was significantly upregulated in the resistant rice genotype upon pathogen infection. Subcellular localization studies suggested that the majority of CBP60 proteins are nuclear-localized, indicating a potential role as transcription factors. Promoter analysis identified diverse stress-responsive cis-regulatory elements in the promoters of CBP60 genes, highlighting their regulatory potential under stress conditions. The upregulation of OsCBP60bcd-1 in response to Xanthomonas oryzae and the presence of stress-responsive elements in its promoter underscore the importance of CBP60 genes in pathogen defense. These findings provide a basis for further investigation into the functional roles of CBP60 genes in crop disease resistance, with implications for enhancing stress resilience in agricultural species.

Bakanae Disease Resistance in Rice: Current Status and Future Considerations

Bakanae disease is mainly caused by Fusarium fujikuroi and is a significant fungal disease with a number of disastrous consequences. It causes great losses in rice production. However, few studies have focused on the details of bakanae disease resistance in rice. Here, we summarize and discuss the progress of bakanae disease resistance in rice. Besides rice germplasm screening and resistance-related gene/quantitative trait locus (QTL) exploration, the route of pathogen invasion in rice plants was determined. We further discussed the regulation of phytohormone-related genes and changes in endogenous phytohormones in rice plants that are induced by the pathogen. To achieve better control of bakanae disease, the use of natural fungicides was assessed in this review. During rice—F. fujikuroi interactions, the infection processes and spatial distribution of F. fujikuroi in infected seedlings and adult plants exhibit different trends. Fungal growth normally occurs both in resistant and susceptible cultivars, with less abundance in the former. Generally, bakanae disease is seed-borne, and seed disinfection using effective fungicides should always be the first and main option to better control the disease. Besides the friendly and effective measure of using natural fungicides, breeding and utilization of resistant rice cultivars is also an effective control method. To some extent, rice cultivars with low grain quality, indica subspecies, and some dwarf or semi-dwarf rice germplasms are more resistant to bakanae disease. Although no highly resistant germplasms were obtained, 37 QTLs were located, with almost half of the QTLs being located on chromosome 1. Using omics methods, WRKYs and MAPKs were usually found to be regulated during rice—F. fujikuroi interactions. The regulation of certain phytohormone-related genes and changes in some endogenous phytohormones induced by the pathogen were clear, i.e., it downregulated gibberellin-related genes and repressed endogenous gibberellins in resistant genotypes, but the opposite results were noted in susceptible rice genotypes. Overall, exploring resistant germplasms or resistance-related genes/QTLs for the breeding of rice with bakanae disease resistance, expanding research on the complex mechanism of rice—F. fujikuroi interactions, and using cost-effective and eco-friendly innovative control methods against the disease are necessary for present and future bakanae disease management.

Melatonin induces acclimation to heat stress and pollen viability by enhancing antioxidative defense in rice (Oryza sativa L.)

Melatonin is a signalling molecule in plants which act as a growth regulator and stress buffering agent but little is known about its defensive role in heat stress tolerance in rice. In this study we analysed the acclimation response of two contrasting heat responsive rice genotypes NERICA-L-44 (NL-44, heat stress tolerant) and Pusa Basmati 1121 (PB-1121, heat stress susceptible) to exogenous melatonin treatment under heat stress. Heat stress induces melatonin biosynthesis in both the genotypes and maximum increase was observed in NL-44. Application of exogenous melatonin further increases melatonin biosynthesis and endogenous content significantly in PB-1121. To understand melatonin’s role in alleviating effects of heat stress, some of the heat stress associated traits such as membrane stability index, hydrogen peroxide content, malondialdehyde content, total antioxidant capacity and enzymatic antioxidants like superoxide dismutase, catalase, ascorbate peroxidase and peroxidase were analysed. We observed that melatonin significantly enhanced membrane stability by reducing hydrogen peroxide mediated membrane damage. Higher activity of antioxidative enzymes and increased total antioxidant capacity helped to maintain reactive oxygen species homeostasis by scavenging mechanism. Exogenous melatonin increased pollen viability, spikelet fertility and grain yield under heat stress treatment. The findings of this study indicates that heat stress induced increase in melatonin biosynthesis may be involved in heat stress tolerance in rice. Exogenous melatonin treatment can further improve heat stress tolerance in susceptible rice genotype (PB-1121) by activating antioxidative mechanism.

Impact of salinity on protein metabolism and enzyme activities in salt-tolerant and susceptible rice genotypes

Impact of salinity on protein metabolism and enzyme activities in salt-tolerant and susceptible rice genotypes

Unraveling the transcriptional network regulated by miRNAs in blast-resistant and blast-susceptible rice genotypes during Magnaporthe oryzae interaction

The plant pathogen Magnaporthe oryzae poses a significant threat to global food security, and its management through the cultivation of resistant varieties and crop husbandry practices, including fungicidal sprays, has proven to be inadequate. To address this issue, we conducted small-RNA sequencing to identify the roles of miRNAs and their target genes in both resistant (PB1637) and susceptible (PB1) rice genotypes. We confirmed the expression of differentially expressed miRNAs using stem-loop qRT-PCR analysis and correlated them with rice patho-phenotypic and physio-biochemical responses. Our findings revealed several noteworthy differences between the resistant and susceptible genotypes. The resistant genotype exhibited reduced levels of total chlorophyll and carotenoids compared to the susceptible genotype. However, it showed increased levels of total protein, callose, H2O2, antioxidants, flavonoids, and total polyphenols. Additionally, among the defense-associated enzymes, guaiacol peroxidase and polyphenol oxidase responses were higher in the susceptible genotypes. In our comparative analysis, we identified 27 up-regulated and 43 down-regulated miRNAs in the resistant genotype, while the susceptible genotype exhibited 44 up-regulated and 62 down-regulated miRNAs. Furthermore, we discovered eight up-regulated and five down-regulated miRNAs shared between the resistant and susceptible genotypes. Notably, we also identified six novel miRNAs in the resistant genotype and eight novel miRNAs in the susceptible genotype. These novel miRNAs, namely Chr8_26996, Chr12_40110, and Chr12_41899, were found to negatively correlate with the expression of predicted target genes, including Cyt-P450 monooxygenase, serine carboxypeptidase, and zinc finger A20 domain-containing stress-associated protein, respectively. The results of our study on miRNA and transcriptional responses provide valuable insights for the development of future rice lines that are resistant to blast disease. By understanding the roles of specific miRNAs and their target genes in conferring resistance, we can enhance breeding strategies and improve crop management practices to ensure global food security.

Phenotyping for the Seedling Stage of the Drought Stress Tolerance in Rice (Oryza sativa L.)

The ever-increasing demand for rice raises the need to increase productivity by developing drought-tolerant rice varieties. Drought tolerance is a complex polygenic trait that largely depends upon plant developmental stages and showed genotype-specific variability. The experiment was conducted using drought tolerant (Binadhan-19, BRRI dhan83) and drought susceptible (BRRI dhan26, BRRI dhan48) rice genotypes at the glasshouse of Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh to characterize the seedling stage drought tolerance as well as disclose the variability of the genotypes for morphological and biochemical traits. A completely randomized design (CRD) with three replications and treatments (control and drought stress imposed by 20% PEG) was used for the experiment. Data on shoot length, root length, fresh root weight, fresh shoot weight, dry root weight, dry shoot weight, chlorophyll content (SPAD value), MDA, and H2O2 were recorded and analyzed through appropriate breeding tools. Significant variation (p<0.01) was observed for genotype, treatment, and genotype (G) × treatment (T) interactions viz., shoot length, root length, root fresh weight, shoot fresh weight, root dry weight, shoot dry weight, chlorophyll, H2O2, and MDA. Drought stress leads to a significant decrease in root and shoots growth whereas the level of H2O2 and MDA increased significantly. A greater decrease in root and shoot growth was observed in susceptible genotypes (BRRI dhan28, BRRI dhan48) compared to tolerant genotypes (BRRI dhan71, Binadhan-19). In contrast, a limited increase in H2O2 and MDA was recorded in tolerant genotypes compared to susceptible genotypes. H2O2 showed a significant positive correlation with root and shoot characteristics under control conditions, whereas H2O2 showed a significant negative correlation with chlorophyll content under drought conditions. MDA showed a significant negative correlation with most of the studied traits under well-watered conditions. Considering all of the traits at the seedling stage, the genotype Binadhan-19 is considered a drought-tolerant genotype both under well-watered and drought conditions, and this genotype was selected for further study under direct field conditions as well as for genetic improvement against drought stress.

Comparative Root Morpho-Anatomical Studies On Meloidogyne Graminicola Resistant And Susceptible Rice Genotypes

The work characterizes root morpho-anatomical changes in rice due to infestation of rice root-knot nematode, Meloidogyne graminicola. The susceptible rice genotype, Satabdi (IET-4786) and resistant rice genotypes Marichsal and Lakshmansal were inoculated with three different geographic isolates of M. graminicola viz., Mg1 (Ashui, West Medinipur), Mg2 (Sumitrapur, West Medinipur) and Mg3 (Gayeshpur, Nadia). The results of demonstrated that, transverse section of morpho-anatomical structure of Mg1 isolate inoculated root at 25 DAI to susceptible and resistant rice genotypes viz., Satabdi and Marichsal showed presence of adult females and egg-mass in the cortex which symbolized a successful host parasitic relationship. Organization of cellular architecture of adventitious root of rice genotypes Satabdi and Marichsal when inoculated with Mg2 isolate then nematode infestation led to drastic changes as evidenced by the disorganization of cortical cells along with dissolution of epidermal, exodermal and sclerenchymatous cells at 10 DAI. The rice genotype, Lakshmansal did not reveal any changes in the root morpho-anatomy when inoculated with Mg2 isolate at 10 DAI. The cross section of the roots of Satabdi and Marichsal revealed complete obliteration of root anatomical structures along with formation of multinucleate giant cells, cavities in the stele owing to nematode injury along with females and egg-masses at 10 and 25 DAI of Mg3 isolate. The rice genotype Lakshmansal, was characterized as resistant against all three isolates of Mg as confirmed by histological study of roots. Cortical thickness of uninfected rice roots was comparatively lesser than infected ones, while maximum stele diameter was recorded with Marichsal when inoculated with Mg1 isolate i.e. 523.01 µm at 25 DAI. Outer portion of the root was also less than infected ones. Giant cell diameter of Satabdi and Marichsal ranged from 43.45-107.46 µm and 30.68-102.67 µm, respectively, indicating successful establishment of host-nematode interaction.

The Expanded and Diversified Calmodulin-Binding Protein 60 (CBP60) Family in Rice (Oryza sativa L.) Is Conserved in Defense Responses against Pathogens

Plant disease management is key to sustainable production of staple food crops. Calcium (Ca2+) signal and phytohormones play critical roles in regulating plant defense responses against pathogens. The Ca2+ signals are sensed, decoded and transduced by calmodulin and other Ca2+ -binding proteins, followed by interaction with and modulation of activities of target proteins such as calmodulin-binding proteins (CBPs). Members of the Arabidopsis CBP60 gene family, AtCBP60g and AtSARD1, have emerged as major regulators of immune responses. In this study, we identified a 15 member CBP60 gene family in rice (Oryza sativa) of which OsCBP60g-3, OsCBP60g-4, OsCBP60a and OsSARD-like1 genes were consistently upregulated in rice seedlings in response to infection with both fungal (Magnaporthe oryzae) and bacterial (Xanthomonas oryzae) pathogens as well as by salicylic acid (SA). OsCBP60g-4 and OsCBP60g-3 were induced maximally by SA and brassinosteroid (BR), respectively, and OsCBP60g-4 was expressed at 3-fold higher levels in the M. oryzae resistant rice genotype (IC-346004) as compared to the susceptible rice genotype (Rajendra Kasturi). The considerable expansion of the immunity clade and the up-regulation of several OsCBP60 genes in response to pathogens and defense hormones supports the importance of further investigating OsCBP60 genes as targets for increasing disease resistance in rice.

Transcriptomic analysis of bakanae disease resistant and susceptible rice genotypes in response to infection by Fusarium fujikuroi.

Fusarium fujikuroi causing bakanae is one of the most significant pathogens of rice and much responsible for yield losses thereby emerging as a major risk to food security. In the present study transcriptomic analysis was conducted between two contrasting resistant (C101A51) and susceptible (Rasi) genotypes of rice with the combinations of C101A51 control (CC) vs. C101A51 inoculated (CI); Rasi control (RC) vs. Rasi inoculated (RI) and C101A51 inoculated (CI) vs. Rasi inoculated (RI). In CC vs. CI commonly expressed genes were 12,764. Out of them 567 (4%) were significantly upregulated and 1399 (9%) genes were downregulated. For the RC vs. RI 14, 333 (79%) genes were commonly expressed. For CI vs. RI 13,662 (72%) genes were commonly expressed. Genes related to cysteine proteinase inhibitor 10, disease resistance protein TAO1-like, oleosin 16kDa-like, pathogenesis-related protein (PR1), (PR4), BTB/POZ and MATH domain-containing protein 5-like, alpha-amylase isozyme were upregulated in resistant genotype C101A51. Whereas, genes related to GDSL esterase/lipase, serine glyoxylate aminotransferase, CASP-like protein 2C1, WAT1-related protein, Cytoplasmic linker associated proteins, xyloglucan endotransglucosylase/hydrolase protein and β-D xylosidase 7 were upregulated in susceptible genotype Rasi. Gene ontology analysis showed functions related to defence response (GO:0006952), regulation of plant hypersensitive type response (GO:0010363), Potassium ion transmembrane activity (GO:0015079), chloroplast (GO:0009507), response to wounding (GO:0009611), xylan biosynthetic process (GO:0045492) were upregulated in resistant genotype C101A51 under inoculated conditions. Real time PCR based validation of the selected DEGs showed that the qRT-PCR was consistent with the RNA-Seq results. This is the first transcriptomic study against bakanae disease of rice in Indian genotypes. Further, functional studies on identified genes and their utilization through different methodology will be helpful for the development of bakanae disease management strategies.

Response of rice genotypes to rice root‐knot nematode (Meloidogyne graminicola) infection under varying temperature regimes

AbstractThe rice root‐knot nematode (RRKN), Meloidogyne graminicola, is one of the major pests of the rice–wheat cropping system. Resistance against M. graminicola in rice could be most valuable in alleviating this problem. The host response of 75 Oryza genotypes was examined at three day/night temperature regimes, 29/26°C, 34/31°C and 38/35°C, in Pluronic gel as well as in soil. Out of the 75 selected rice genotypes, only Zhenshan 97 B exhibited high resistance to this set of temperature regimes, with the least number of galls/root system. At 34/31°C, more second‐stage juveniles (J2s) were hatched and J2 population densities in roots of the susceptible rice genotypes increased significantly compared with those of plants grown at the 29/26°C. In resistant genotypes, only a few J2s penetrated roots and developed into mature females. The histopathological studies revealed that in susceptible rice genotypes at high‐temperature regimes, the multinucleate giant cells were well developed. The results presented in this study revealed that an increase in temperature had a significant effect on the resistance of rice genotypes and resistance appeared more pronounced in genotype Zhenshan 97 B. This resistant genotype can be used in marker‐assisted selection to develop resistant elite cultivars.

Integrated Metabarcoding and Culturomic-Based Microbiome Profiling of Rice Phyllosphere Reveal Diverse and Functional Bacterial Communities for Blast Disease Suppression.

Phyllosphere—the harsh foliar plant part exposed to vagaries of environmental and climatic variables is a unique habitat for microbial communities. In the present work, we profiled the phyllosphere microbiome of the rice plants using 16S rRNA gene amplicon sequencing (hereafter termed metabarcoding) and the conventional microbiological methods (culturomics) to decipher the microbiome assemblage, composition, and their functions such as antibiosis and defense induction against rice blast disease. The blast susceptible rice genotype (PRR78) harbored far more diverse bacterial species (294 species) than the resistant genotype (Pusa1602) that showed 193 species. Our metabarcoding of bacterial communities in phyllomicrobiome revealed the predominance of the phylum, Proteobacteria, and its members Pantoea, Enterobacter, Pseudomonas, and Erwinia on the phyllosphere of both rice genotypes. The microbiological culturomic validation of metabarcoding-taxonomic annotation further confirmed the prevalence of 31 bacterial isolates representing 11 genera and 16 species with the maximum abundance of Pantoea. The phyllomicrobiome-associated bacterial members displayed antifungal activity on rice blast fungus, Magnaporthe oryzae, by volatile and non-volatile metabolites. Upon phyllobacterization of rice cultivar PB1, the bacterial species such as Enterobacter sacchari, Microbacterium testaceum, Pantoea ananatis, Pantoea dispersa, Pantoea vagans, Pseudomonas oryzihabitans, Rhizobium sp., and Sphingomonas sp. elicited a defense response and contributed to the suppression of blast disease. qRT-PCR-based gene expression analysis indicated over expression of defense-associated genes such as OsCEBiP, OsCERK1, and phytohormone-associated genes such as OsPAD4, OsEDS1, OsPR1.1, OsNPR1, OsPDF2.2, and OsFMO in phyllobacterized rice seedlings. The phyllosphere bacterial species showing blast suppressive activity on rice were found non-plant pathogenic in tobacco infiltration assay. Our comparative microbiome interrogation of the rice phyllosphere culminated in the isolation and identification of agriculturally significant bacterial communities for blast disease management in rice farming through phyllomicrobiome engineering in the future.

Characterization of evolutionarily distinct rice BAHD-Acyltransferases provides insight into their plausible role in rice susceptibility to Rhizoctonia solani.

Plants produce diverse secondary metabolites in response to different environmental cues including pathogens. The modification of secondary metabolites, including acylation, modulates their biological activity, stability, transport, and localization. A plant-specific BAHD-acyltransferase (BAHD-AT) gene family members catalyze the acylation of secondary metabolites. Here we characterized the rice (Oryza sativa L.) BAHD-ATs at the genome-wide level and endeavor to define their plausible role in the tolerance against Rhizoctonia solani AG1-IA. We identified a total of 85 rice OsBAHD-AT genes and classified them into five canonical clades based on their phylogenetic relationship with characterized BAHD-ATs from other plant species. The time-course RNA sequencing (RNA-seq) analysis of OsBAHD-AT genes and qualitative real-time polymerase chain reaction (qRT-PCR) validation showed higher expression in sheath blight susceptible rice genotype. Furthermore, the DNA methylation analysis revealed higher hypomethylation of OsBAHD-AT genes that corresponds to their higher expression in susceptible rice genotype, indicating epigenetic regulation of OsBAHD-AT genes in response to R. solani AG1-IA inoculation. The results shown here indicate that BAHD-ATs may have a negative role in rice tolerance against R. solani AG1-IA possibly mediated through the brassinosteroid (BR) signaling pathway. Altogether, the present analysis suggests the putative functions of several OsBAHD-AT genes, which will provide a blueprint for their functional characterization and to understand the rice-R. solani AG1-IA interaction.

Biochemical Profiling of Resistant Phenotypes Against Bipolaris oryzae Causing Brown Spot Disease in Rice

Brown leaf spot disease of rice is a dominant lethal disease, caused by the fungus Bipolaris oryzae. The pathogen is an obligate parasite and causes qualitative and quantitative damage to rice crop. The objective of the present study was to investigate what extent the defense related biochemical compounds reflect the distinct categories of resistance phenotypes in rice against brown spot disease. This was done by determining the concentration of Catalase (CAT), Phenylalanine ammonia-lyase (PAL), Polyphenol oxidase (PPO), Peroxidase (POD), and β-1,3-Glucanase enzymes in resistant, moderately resistant and susceptible rice genotypes. The disease resistant phenotypes in rice line (PARC-7) reflect the higher accumulation of CAT, PAL, PPO, POD, and β-1,3-Glucanase. The pattern of enzyme accumulation was similar in all resistant genotypes. The rice genotypes with moderately resistant phenotypes showed significant difference with respect to the concentration of biochemical defense-related compounds. The difference in accumulation of defense related enzymes reflected the level of disease severity (% leaf area covered) on resistant and moderately resistant genotypes. The susceptible rice genotypes showed the minimum concentration of these enzymes, with the lowest concentrations found in the rice variety Bas-2000 (80% Disease Index). The differential defense response in resistant and susceptible genotypes suggests that these enzymes can be used as biochemical markers for early detection of disease resistant genotypes. The study of enzyme accumulation at different time points and at different levels of disease severity helps to understand the resistance mechanisms against brown spot disease in rice.

Identification and expression analysis of pathogenicity-related genes of Rhizoctonia solani anastomosis groups infecting rice.

The online version contains supplementary material available at 10.1007/s13205-021-02934-1.

Comparative transcriptome profiling reveals the basis of differential sheath blight disease response in tolerant and susceptible rice genotypes.

Rice sheath blight (ShB) disease, caused by the fungal pathogen Rhizoctonia solani AG1-IA, is one of the devastating diseases and causes severe yield losses all over the world. No completely resistant germplasm is known till now, and as a result, the progress in resistance breeding is unsatisfactory. Basic studies to identify candidate genes, QTLs, and to better understand the host-pathogen interaction are also scanty. In this study, we report the identification of a new ShB-tolerant rice germplasm, CR 1014. Further, we investigated the basis of tolerance by exploring the disease responsive differentially expressed transcriptome and comparing them with that of a susceptible variety, Swarna-Sub1. A total of 815 and 551 genes were found to be differentially regulated in CR 1014 and Swarna-Sub1, respectively, at two different time points. The result shows that the ability to upregulate genes for glycosyl hydrolase, secondary metabolite biosynthesis, cytoskeleton and membrane integrity, the glycolytic pathway, and maintaining photosynthesis make CR 1014 a superior performer in resisting the ShB pathogen. We discuss several putative candidate genes for ShB resistance. The present study, for the first time, revealed the basis of ShB tolerance in the germplasm CR1014 and should prove to be particularly valuable in understanding molecular response to ShB infection. The knowledge could be utilized to devise strategies to manage the disease better.

A 1.84-Mb region on rice chromosome 2 carrying SPL4, SPL5 and MLO8 genes is associated with higher yield under phosphorus-deficient acidic soil.

Phosphorus (P) deficiency is one of the major limiting factors for rice productivity with only one locus (PSTOL1) available for field based application. A biparental mapping population (F6) derived from two P deficiency tolerant genotypes (Sahbhagi Dhan (SD) (PSTOL1+) and Chakhao Poreiton (CP) (PSTOL1-)), in which, transcriptome data generated from our lab had previously shown existence of diverse mechanisms was used to identify novel regions for better yield under lowland acidic soils. Phenotyping at F4, F5 and F6 generations revealed significant correlation between traits like tiller number at 30days (TN 30), tiller number at 60days (TN 60), filled grains (FG), percent spikelet fertility (SF%), panicle number (PN) and grain yield per panicle (GYPP) and also association with better yield/performance under low P acidic soil conditions. Through selected genotyping on a set of forty superior and inferior lines using SSR, candidate gene-based and SNP polymorphic markers, 5 genomic regions associated with various yield-related traits were identified. Marker trait association studies revealed 13 markers significantly associated with yield attributing traits and PUE under lowland acidic field conditions. Chi-square and regression analyses of markers run on the entire population identified seven and six markers for SF% and GYPP, respectively, and two for biological yield with positive allele derived from SD which constitute a novel 1.847-Mb region on chromosome 2 flanked by two markers RM12550 and PR9-2. Expression analysis of 7 candidate genes lying within this region across SD, CP and two low P susceptible rice genotypes has revealed that expression of four genes including SPL4, SPL5, ACA9 and MLO8 is significantly upregulated only in SD under low P conditions. In CP, there is low expression of MLO8 under low P conditions, whereas SPL4, SPL5 and Os02g08120 are downregulated. In the case of the two susceptible genotypes, there is no expression of Os02g08120 either in optimum or limiting conditions. Sequence data across a panel of 3024 rice genotypes also suggests that there is polymorphism for these differentially expressed genes. The genes and underlying markers identified on chromosome 2 will be key to imparting tolerance to low P in diverse genetic backgrounds and for marker-assisted selection for higher yield under lowland acidic conditions.

Role of sedoheptulose-1,7 bisphosphatase in low light tolerance of rice (Oryza sativa L.).

Rice grain yield is drastically reduced under low light especially in kharif (wet) season due to cloudy weather duringmost part of crop growth. Therefore, 50-60% of yield penalty was observed. To overcome this problem, identification of low light tolerant rice genotypes with a high buffering capacity trait such as photosynthetic rate has to be developed. Sedoheptulose-1,7 bisphosphatase, a light-regulated enzyme, plays pivotal role in the Calvin cycle by regenerating the substrate (RuBP) for RuBisCo and therefore, indirectly regulates the influx of CO2 for this crucial process. We found a potential role of SBPase expression and activity in low light tolerant and susceptible rice genotypes by analyzing its influence on net photosynthetic rate and biomass. We observed a significant relationship of yield with photosynthesis, SBPase expression and activity especially under low light conditions. Two tolerant and two susceptible rice genotypes were used for the present study. Tolerant genotypes exhibited significant butleast reduction compared to susceptible genotypesin the expression and activity of SBPase, which was also manifested in its photosynthetic rate and finally in the grain yield under low light. However, susceptible genotypes showed significant reduction in SBPase activity along with photosynthesis and grain yield suggesting that tracking the expression and activity of SBPase could form a simple and reliable method to identify the low light tolerant rice cultivars. The data were analyzed using theIndostat 7.5, Tukey-Kramer method through Microsoft Excel 2019 and PAST4.0 software. The significant association of SBPase activity with the grain yield, net assimilation rate, electron transfer rate, biomass and grain weight were observed under low light stress. These traits should be considered while selecting and breeding for low light tolerant cultivars. Thus, SBPase plays a major role in the low light tolerance mechanism in rice.

Lipidomic analyses reveal enhanced lipolysis in planthoppers feeding on resistant host plants.

The brown planthopper (BPH) (Nilaparvata lugens Stål) is a highly destructive pest that seriously damages rice (Oryza sativa L.) and causes severe yield losses. To better understand the physiological and metabolic mechanisms through which BPHs respond to resistant rice, we combined mass-spectrometry-based lipidomics with transcriptomic analysis and gene knockdown techniques to compare the lipidomes of BPHs feeding on either of the two resistant (NIL-Bph6 and NIL-Bph9) plants or a wild-type, BPH susceptible (9311) plant. Insects that were fed on resistant rice transformed triglyceride (TG) to phosphatidylcholine (PC) and digalactosyldiacylglycerol (DGDG), with these lipid classes showing significant alterations in fatty acid composition. Moreover, the insects that were fed on resistant rice were characterized by prominent expression changes in genes involved in lipid metabolism processes. Knockdown of the NlBmm gene, which encodes a lipase that regulates the mobilization of lipid reserves, significantly increased TG content and feeding performance of BPHs on resistant plants relative to dsGFP-injected BPHs. Our study provides the first detailed description of lipid changes in BPHs fed on resistant and susceptible rice genotypes. Results from BPHs fed on resistant rice plants reveal that these insects can accelerate TG mobilization to provide energy for cell proliferation, body maintenance, growth and oviposition.

Biochemical and molecular variability of Fusarium fujikuroi isolates and their differential interactions with rice genotypes during infection

Bakanae of rice caused by Fusarium fujikuroi has become an important disease in major rice production regions worldwide. Twenty-nine isolates of F. fujikuroiwere differentiated based on produced pigments, gibberellic acid (GA3), molecular characteristics and correlated with bakanae disease symptomsof rice seedling. All isolates of F. fujikuroi produced pigments except six isolates. Also, all isolates differed in the production of gibberellic acid. The isolate FI 12 was the highest produced for GA3 and disease severity index. Molecular analysis showed that nine ISJ primers produced a total of 809 amplified bands. Current results indicated that the Fusarium isolates were clustered largely based on location and/or severity of isolates and level of GA3 production. The molecular results obtained proved the existence of high levels of genetic variation and high-resolution power of ISJ marker in detecting Fusarium molecular diversity. The differential behavior of bakanae pathogen in resistant and susceptible rice genotypes during infection was studied. Under greenhouse condition, Giza 177 was highly susceptible to rice genotypes while Giza 179 and Giza 178 were moderately resistant. Biochemical changes such as antioxidant enzymes and hydrogen peroxide (H2O2)was observed in rice seedling after 7, 15 and 21 days from inoculation. Enzymes activities were increased in Giza 179 and Giza 178 genotypes, but in H2O2 content was decreasing. Under field conditions, all genotypes showed increased chlorophyll content, number of tillers/hills and means of panicle length/10 healthy plants compared with infected plants. Giza 179 showed the highest grain yield.

ITRAQ proteomics reveals the regulatory response to Magnaporthe oryzae in durable resistant vs. susceptible rice genotypes.

Rice blast disease caused by Magnaporthe oryzae (M. oryzae) is one of the most serious diseases. Although previous research using two-dimensional gel-based proteomics to assess the proteins related to the rice blast resistance had been done, few proteins were identified. Here, we used the iTRAQ method to detect the differentially expressed proteins (DEPs) in the durable resistant rice variety Gangyuan8 (GY8) and the susceptible rice variety Lijiangxintuanheigu (LTH) in response to M. oryzae invasion, and then transcriptome sequencing was used to assist analysis A total of 193 and 672 DEPs were specifically identified in GY8 and LTH, respectively, with only 46 similarly expressed DEPs being shared by GY8 and LTH.39 DEPs involved in plant-pathogen interaction, plant hormone signal transduction, fatty acid metabolism and peroxisome biosynthesis were significantly different between compatible interaction (LTH) and incompatible interaction (GY8). Some proteins participated in peroxide signal transduction and biosynthesis was down-regulated in GY8 but up-regulated in LTH. A lot of genes encoding pathogenesis-related gene (PR), such as chitinase and glucanase, were significantly up-regulated at both the transcriptome and proteome levels at 24 hours post-inoculation in GY8, but up-regulated at the transcriptome level and down-regulated at the proteome level in LTH. Our study reveals that the pathogen-associated molecular pattern (PAMP)-triggered immunity defense system may be activated at the transcriptome level but was inhibited at the protein level in susceptible rice varieties after inoculation. The results may facilitate future studies of the molecular mechanisms of rice blast resistance.