Adult Survival Rates Research Articles

Differences in disease characteristics in young vs older adults with colorectal carcinoma.

34 Background: Colorectal carcinoma (CRC) exhibits distinct disease characteristics between younger (≤50 years) and older (>50 years) adults, which may significantly influence treatment decisions and outcomes. This study aims to investigate differences in disease characteristics of colorectal carcinoma among patients aged ≤50 years compared to those aged >50 years, utilizing data from the Surveillance, Epidemiology, and End Results (SEER) database. Methods: A retrospective analysis was performed using the SEER database covering the years 2000 to 2020. A total of 150,000 diagnosed CRC cases were included. Key variables analyzed comprised stage at diagnosis, tumor grade, histological subtype, and 5-year survival rates. Rates of CRC diagnoses in various age brackets were calculated for three time periods: 2000-2010, 2010-2020, and overall from 2000-2020. Results: Within the total cohort, 12% (18,000 cases) were diagnosed in young adults, while 88% (132,000 cases) were in older adults. An analysis of age-specific rates showed: 2000-2010 : Young adults (≤50 years) represented 10% of CRC cases, with 16% presenting at advanced stages (Stage III or IV). 2010-2020 : This group increased to 12%, with 65% diagnosed at advanced stages (Stage III or IV), compared to 52% in older adults (p < 0.001). In terms of tumor characteristics, poorly differentiated tumors were more prevalent in young adults (28% vs. 18%, p < 0.001). The prevalence of microsatellite instability-high (MSI-H) tumors was significantly greater in young adults at 18%, compared to 12% in older adults (p < 0.05). The 5-year overall survival rate for young adults was 68%, notably higher than the 48% for older adults (p < 0.001). Conclusions: This study underscores significant differences in disease characteristics between young and older adults with colorectal carcinoma. Young patients are more likely to present with advanced disease but have better survival outcomes. These findings indicate the necessity for an age specific approach to screening and treatment strategies. Differences in stage at diagnosis, tumor grade, presence of MSI-H, and survival outcomes between younger and older adults diagnosed with colorectal carcinoma. Category Young Adults (≤50 years) Older Adults (>50 years) p-value Proportion of Total CRC Cases 12% (18,000 cases) 88% (132,000 cases) Advanced Stage (Stage III or IV) 65% (2010-2020) 52% (2010-2020) < 0.001 Poorly Differentiated Tumors 28% 18% < 0.001 Microsatellite Instability-High (MSI-H) 18% 12% < 0.05 5-Year Overall Survival Rate 68% 48% < 0.001

Apparent survival and population turnover in a long-lived generalist raptor: a comparison of estimation methods

Adult survival and turnover rates are often estimated in long-lived species as the primary drivers of population dynamics. Various methods have been used, yet comparison of estimation methods is seldom undertaken. To address this gap, we used colour-ringing across different age groups, as well as GPS-tracking and genetic analysis of shed feathers, to estimate these critical parameters in a population of the medium-sized bird of prey, the Lesser Spotted Eagle. According to the colour-ringing data, the apparent survival during the first year of life (0.21) was more than four times lower than that of birds older than one year (0.88); the GPS-tracking (0.84) and genetic analysis (0.81) yielded similar survival estimates. All used techniques indicated a stable trend in apparent survival rates. However, population turnover rates derived from genetic feather analysis (0.17–0.43) exceeded expectations based on apparent survival estimates, as well as those derived from colour-rings (0.05–0.17) and GPS-tracking (0.05–0.08). Notably, all markers indicated high site fidelity among adult eagles, with breeding dispersal events being infrequent. Our findings underscore the utility of diverse methods for estimating apparent survival, though the ecological characteristics of the species may influence the efficacy of each method. However, estimates of population turnover rates were notably influenced by the choice of method and should be interpreted cautiously before recommending management actions.

Influences of light-dark cycle and water temperature on in vitro egg laying, hatching, and survival rate of the Thaparocleidus vistulensis (Dactylogyridea: Ancylodiscoididae).

The European catfish (Silurus glanis) is an important species with high economic value, and its growing demand has led to intensive farming practices for it. However, this species is increasingly challenged by parasitic infections, particularly from a specific gill monopisthocotylan parasite called Thaparocleidus vistulensis. To establish effective management strategies, it is crucial to comprehend the fundamental environmental variables that could influence the reproductive and survival behavior of T. vistulensis. The present study conducted controlled in vitro experiments to observe the fecundity, hatching rate, and survival ability of T. vistulensis under varying light-dark conditions, as well as across a range of water temperatures from 5 to 35°C. Interestingly, light exposure led to a threefold increase in egg production by adult T. vistulensis compared to constant darkness. While light or dark conditions did not significantly affect the hatching and survival rate of adults, they did significantly affect the survival rate of oncomiracidia. The parasite's fecundity was optimal at 15°C. Eggs hatched fastest at 30 °C, whereas no hatching occurred at 5°C and 35 °C. The survival rate for both oncomiracidia and adults showed a negative correlation with increasing water temperatures. These findings provide fundamental insights into how varying environmental variables impact the life cycle of the parasite. The improved understanding of these findings provides a valuable basis for the management of T. vistulensis in cultured European catfish stocks in fish farms.

Outcome from out-of-hospital cardiac arrest managed by the pre-hospital emergency medical system in Martinique, a French Caribbean Overseas Territory.

Out-of-hospital cardiac arrest (OHCA) affects approximately 46,000 people in France annually and survival remains low. There is no published data specific to the characteristics and outcomes of OHCA in French overseas territories, especially in the French Caribbean territories. The aim of this study was to describe the characteristics and outcomes of adult OHCA patients managed by the Emergency Medical Service team (EMS) in Martinique. All adults with OHCA, managed by the EMS of Martinique between January 1st 2018 and June 30th 2019, were included. Primary outcome was 30day-survival and neurological outcome at 30days assessed by the Cerebral Performance Category scale (CPC). Secondary outcomes were return of spontaneous circulation (ROSC) prior to hospital admission and causes of cardiac arrest in patients with ROSC. This study included 340 OHCA patients. The population was predominantly male (64%), with a median age of 68 [54-78] years. OHCA resulted from a medical condition in 314 patients (92%) and occurred mainly at home (75%), in the presence of witnesses for 235 patients (69%). Basic life support was initiated in 174 OHCA (51%). Median time to first-responders' and prehospital mobile intensive care unit's arrivals at scene were 17 [10-30] and 27 [19-41] minutes after call to the EMS dispatching center for OHCA. Non-shockable initial rhythm was present in 315 patients (93%), and 240 patients (71%) received advanced life support. Thirty-one patients (9%) achieved ROSC. On day 30, 13 patients (3.8%) were still alive, and 8 of them (2.4%) were alive with a CPC score of 1 or 2. The overall adult OHCA survival rate and survival with good neurological status on day-30 in the French Caribbean island of Martinique are low. OHCA survival rate may be improved by educating the population on basic life support techniques and reducing the time responses for first-responders and prehospital mobile intensive care unit to reach patients.

Survival of White-Tailed Eagles Tracked after Rehabilitation and Release

ABSTRACT There are many raptor rehabilitation centers around the world that receive and care for wild raptors. Wildlife rehabilitation is a labor- and cost-intensive part of addressing animal welfare. Apart from the compassionate aspect of taking responsibility for an injured animal, there remains an open question as to whether doing so contributes to animal conservation. The fates of such animals after their rehabilitation and release have rarely been studied. Long-term post-release monitoring is needed to assess survival and reproduction. Here, we investigate the post-release fates of 22 White-tailed Eagles (Haliaeetus albicilla), including juveniles, immature birds, and adults in Austria, the Czech Republic, Germany, and Slovakia. Using GPS tracking, we monitored the eagles for 2–2639 d (median = 340 d) between 2005 and 2023. The longest-surviving eagle was tracked for more than 7 yr. Release was followed by a short 6-d period of high mortality, followed in turn by a long-term annual adult survival rate of 74.6%. Anthropogenic factors contributed to 70% of the long-term mortality cases. Reason for admission, length of hospital stay, and use of flight aviaries had no statistically significant effect on long-term survival. Five eagles successfully bred and produced a total of 10 fledglings. Traveling as much as 1378 km, the released eagles dispersed throughout central, northern, and eastern Europe. Thus, it can be concluded that successful rehabilitation of White-tailed Eagles resulted in high survival rates; and in five cases, successful reproduction indicated effective reintegration into the wild population.

Survival comparison in adults with congenital systemic to pulmonary shunt and borderline elevated pulmonary vascular resistance versus Eisenmenger syndrome

Pulmonary arterial hypertension (PAH) associated with congenital heart disease (PAH-CHD) is a consequence of unrepaired large systemic-to-pulmonary shunts. The long-term data of adult patients who have PAH-CHD with elevated pulmonary vascular resistance (PVR) are limited. We aimed to investigate the survival of adults who had PAH-CHD with predominantly left-to-right (L-R) shunts with (1) borderline-to-high PVR and (2) treat-and-repair compared with those with Eisenmenger syndrome (ES). From 1995 to 2021, 99 adults with ES (age 34.1 ± 11.2 years) and 118 adults with PAH-CHD with predominantly L-R shunts (age 39.1 ± 13.7 years) were eligible. The PVR in the ES group was 21.0 ± 13.1 WU. Most ES patients (97%) received pulmonary vasodilator therapy. Among the 118 patients with PAH-CHD with predominantly L-R shunts, the baseline PVR was 7.6 ± 4.6 WU, and 78 patients (66.1%) had borderline to high PVR (≥ 5 WU). In the group, 105 patients (88.9%) underwent repair; 84 had defect closure, and 21 had fenestrated closure. Treat-and-repair was used to treat 53 patients with a preoperative final PVR of 3.58 ± 2.63 WU. No early postoperative deaths were reported. At a median follow-up time of 5.4 years (range 0.1–23.6 years), the 10- and 15-year survival rates of adults with borderline PVR were 82.3% and 82.3%, respectively, which were not inferior to the rates for patients with ES, which were 77.8% and 71.2%, respectively (p = 0.41). The survival rate of patients who underwent treat-and-repair was slightly better than that of patients who underwent ES, although the difference was not statistically significant (p = 0.19). Independent mortality risk factors were functional class III-IV at initial presentation (hazard ratio 5.7, 95% CI 1.2–26.6; p = 0.02) and oxygen saturation < 94% at the most recent visit (hazard ratio 9.4, 95% CI 2.1–42.9; p = 0.004).Trial registration: TCTR20200420004.

Survival estimates of GPS-tagged adult Golden Eagles (Aquila chrysaetos) breeding in Finland

Adult survival estimates are important for assessing population status and viability and for investigating the role of different anthropogenic effects on their variation. Currently, the rapidly increasing wind power poses a new and severe threat to survival of large raptors. Between 2011–2024, we monitored 26 Golden eagles (Aquila chrysaetos) using Global Positioning System tracking devices in Finland, on territories where wind-power plants are currently absent and eagles are not subject to increased mortality from wind power plant collisions. Using the accumulated tracking data, we constructed individual capture histories on a monthly basis and adult survival rates using known-fate models in program MARK. We also review published adult survival estimates of Golden Eagles reported throughout their range for relative comparison. Monthly adult survival in our study area was 0.9933 (0.9854–0.9970) which translates to annual survival of 0.924 (0.838–0.965). We found eight studies reporting survival estimates, which ranged from 0.860 to 0.975 with a mean of 0.928. Thus, adult survival rates of Golden Eagles breeding in northern Finland, that are not affected by wind power plants, are high as expected for a viable population and very close to the mean estimated from other Golden Eagle populations. Maintaining high adult survival rates will be key to Golden Eagle population stability with expanding wind energy development in Finland.

Variability and correlations among vital rates and their influence on population growth in mule and black‐tailed deer

AbstractTo reverse range‐wide population declines, managers of black‐tailed and mule deer (Odocoileus hemionus) require information on the vital rates and life stages most influential to population growth to target effective management actions. We extracted black‐tailed and mule deer vital rates from a range‐wide literature review and used hierarchical models to summarize vital rates, their variability, and how they correlate with one another. We then used matrix models and life‐stage simulation analysis to determine the individual vital rates that contributed most to annual population growth rate (i.e., lambda). Annual adult female survival explained the greatest amount of variation (62%) in lambda. Annual juvenile survival explained 44% of the variation in lambda, whereas summer or winter juvenile survival by themselves were far less informative. Winter fawn:doe ratios, a metric often collected by management agencies, explained only 10% of the variation in lambda. Given an adult female survival of 0.84, our simulations estimated a lambda of 1.0 (95% credible interval = 0.88–1.14), indicating equal probability that a population would increase or decrease. Simulations further indicated that given adult survival rates &lt;70%, the population would always decline, but as survival increased lambda increased linearly. In contrast, estimates of lambda plateaued when annual juvenile survival reached approximately 0.5, indicating higher survival rates yielded diminishing returns to population stability. Using simulated values within the observed range of vital rate values across the species' geographical distribution, the mean lambda was 0.975 and in 61% of the simulations, lambda was &lt;1. After 20 years, we estimated that this distribution of lambda values would cause populations to decrease in 92% of instances with a mean decrease of 44%. Our results align with observed declines in mule deer populations throughout their range over recent decades and indicate that these trends will continue until management can improve survival of adult females.

Health-related quality of life and survival of older adults in Campinas, São Paulo, Brazil: A retrospective analysis from 2008 to 2018

As the average life expectancy continues to rise, the prevalence of multimorbidity is also expected to increase, potentially leading to outcomes such as functional decline, a higher risk of premature death, and adverse effects on overall health and well-being. This study aimed to estimate the survival rates of older adults with varying levels of health-related quality of life (HRQoL) and to assess the association between the domains and components of the 36-item Short Form Health Survey and all-cause mortality over a 10-year period in Brazil. We conducted a retrospective longitudinal study using baseline data from 1,520 elders (aged 60 years and older) who participated in the Health Care Survey of the Municipality of Campinas, S&amp;atilde;o Paulo, Brazil (ISACamp 2008/2009). A linkage was established between the ISACamp databases and the Mortality Information System. An active search was performed for individuals whose data could not be paired to confirm the death status. Survival functions were calculated using the Kaplan&amp;ndash;Meier method, while hazard ratios (with 95% confidence intervals) were determined using Cox regression analysis. All HRQoL domains showed proportional hazards and statistically significant differences (p &lt; 0.05) between survival curves, except for the bodily pain domain. In the multivariate analysis, lower scores in physical functioning and role-physical were associated with a 74% and 42% increased risk of death, respectively. In addition, impairments in role-emotional, mental health, and general health heightened the risk of mortality by approximately 36%. Notably, the lowest score in the physical component emerged as a significant predictor of mortality, increasing the probability of death by 47%, while the mental component showed no significant association. Our findings provide compelling evidence of the predictive capacity of HRQoL in evaluating mortality risk among older people in low- and middle-income countries.

Quality of Life and Psychological Distress Related to Fertility and Pregnancy in AYAs Treated for Gynecological Cancer: A Systematic Review.

With growing survival rates for Adolescent and Young Adults (AYAs) diagnosed with gynecological cancer, the focus shifted to Quality of Life (QoL). Fertility-sparing surgery offers a viable alternative to standard, usually fertility-impairing treatments. Treatment choice remains difficult and renders perspectives of AYAs on decision-making and psychological outcomes afterwards. This review examines the impact of (in)fertility on psychological well-being both during cancer treatment, and in the long term. A systematic review of the peer-reviewed literature was conducted by searching Pubmed, Web of Science, Cochrane Trial database and PsycINFO on 30 November 2023. The review included studies with a focus on gynecological cancer, fertility and pregnancy related psychological outcomes, QoL, and psychosocial factors influencing decision-making. Case reports and reviews were excluded. Quality was assessed with the Mixed Methods Appraisal Tool (MMAT). 15 studies, published between 2005 and 2023, involving 1328 participants, were included. Key findings highlight the significance of informing all AYAs about cancer treatment effects on fertility and discussing fertility preservation options. Feeling time-pressured and conflicted between choosing the best oncological outcomes and preserving fertility were common. Factors such as younger age at diagnosis, time pressure, and inadequate counseling by healthcare workers increased reproductive concerns which contributed to long term psychological distress. Research on AYAs with gynecological cancer without fertility preservation possibilities is limited and should be prioritized. This review shows that both Shared Decision-Making (SDM) and follow-up processes can be improved by addressing fertility-related questions and concerns, therefore increasing long-term QoL. This review is registered in PROSPERO (ID 448119).

In Vitro Assessment of Metarhizium Anisopliae Pathogenicity Against Aedes Aegypti Life Stages.

Aedes aegypti transmits the arboviruses that cause dengue, zika, and chikungunya. Entomopathogenic fungi are beneficial microorganisms that can be incorporated into current strategies against mosquitoes of public health concern. This study molecularly identified the Metarhizium anisopliae CG 153 isolate and evaluated its virulence against larvae, pupae, and adults (both males and females) of Ae. aegypti. Different concentrations of conidia were used (1 × 104-8 conidia mL-1). Larval and pupal survival was monitored daily for seven and three days, respectively, while adults were monitored for 15days. The efficacy of M. anisopliae sensu stricto was concentration-dependent, with higher concentrations achieving better results, demonstrating greater virulence against larval and adult stages of Ae. aegypti. The fungus reduced the larval survival by 95,5% (1 × 108 con.mL-1), 94,4% (1 × 107 con.mL-1), 78,9% (1 × 106 con.mL-1), 62,2% (1 × 105 con.mL-1), and 41,1% (1 × 104 con.mL-1) after seven days. Adults also showed susceptibility to the fungus, with no observed difference in susceptibility between males and females. Over 15days of monitoring, adult survival rates ranged from approximately 6.7% to 72%. Pupae exhibited lower susceptibility to the fungus across different concentrations, with survival rates ranging from approximately 87.8% to 100%. This study highlights the high effectiveness of M. anisopliae CG 153 against both Ae. aegypti larvae and adults (male and female) under controlled conditions, suggesting its promising potential for further evaluation and application in field conditions.

Florida sandhill crane survival along a wildland‐to‐urban land use gradient

AbstractThe Florida sandhill crane (Antigone canadensis pratensis) is a state‐threatened non‐migratory subspecies. Our understanding of adult crane survival in Florida, USA, is unclear, as it relies on decades‐old unpublished data of birds residing in natural areas. Since that time, the loss of natural habitat precipitated cranes using urbanized areas such as suburban lawns and roadside verges for foraging and loafing. Contemporary studies are needed to properly guide crane management and conservation efforts. We addressed this knowledge gap by estimating the annual survival rate for sandhill cranes in 12 central Florida counties. We used a live‐dead capture‐recapture multistate model, monitoring 118 adult cranes from June 2017 to May 2023; 76 were color‐banded and 42 were tagged with Global System for Mobile Communications (GSM) transmitters. Fifteen cranes died during the study, with vehicle strikes (n = 9) being the most prevalent identified source of mortality. Overall, the annual survival probability was estimated at 0.79 (95% credibility interval = 0.75–0.84). Using the subset of GSM‐tagged cranes, we observed no influence of urbanization on annual survival rate (βurbanization gradient = 0.007, 95% credibility interval = −0.008, 0.022). The adult survival rates we observed are lower than reported for other populations of sandhill cranes in North America that are considered stable or growing.

Evaluating the effects of short-term low temperature on the growth and development of Trichopria drosophilae based on the age–stage two-sex life table

BackgroundThe effects of low temperatures on parasitic wasps are crucial for maintaining farmland biodiversity and enhancing biological control, especially given the implications of global warming and frequent extreme cold events.MethodsWe studied the effects of different low temperatures (−8 ± 1 °C, −4 ± 1 °C, 0 ± 1 °C, 4 ± 1 °C, and 8 ± 1 °C) on the mating frequency and duration of male adults of Trichopria drosophilae and the number of pupae beaten by female adults, and constructed the age–stage two-sex life table of T. drosophilae.ResultsThis study found that male T. drosophilae adults exposed to low temperatures for 12 h significantly altered their mating behavior, peaking between 15:00 and 17:00. As the temperature dropped during the exposure, both the mating frequency of T. drosophilae and the duration of pupal beating were affected. The survival rate of female adults dropped from 39.55% at 8 °C to just 21.17% at −8 °C. Low-temperature treatment shortened the development period and lifespan for T. drosophilae adults. They developed 4.71 days faster and had a total lifespan that was 10.66 days shorter than those in the control group after being exposed to −8 °C. Furthermore, the average number of eggs laid by females at −8 °C was 4.46 less than that at 8 °C and 6.16 less than that in the control group, which laid an average of 21.55 eggs. The net reproductive rate (R0) of T. drosophilae decreased with lower temperatures, reaching a low of 23.64 at −8 °C. Conversely, the intrinsic growth rate (rm) actually increased as temperatures dropped, with the lowest value being 0.21 at −8 °C.ConclusionsThe findings indicate that short-term exposure to low temperatures hampers the growth and population increase of T. drosophilae, thereby reducing their effectiveness as biological control agents.Graphical

Regional demography of Icelandic rock ptarmigan and its implications for harvest management

Abstract The rock ptarmigan (Lagopus muta) is a popular game bird in Iceland, but management of hunting has tended to be controversial. We were interested in whether regional differences in ptarmigan demography exist, and if so, their implications for harvest management. We fit integrated population models (IPMs) to monitoring data from six hunting regions of Iceland during 2005–2023, and then examined equilibrium and non‐equilibrium harvest strategies based on an objective to maximize sustainable harvest. Survival and reproductive rates tended to be similar amongst regions, except in the demographically important Northeast, where survival was lower and productivity was higher. There was a negative relationship between post‐breeding age ratio (juveniles/adults) and spring abundance in all regions, although the strength of density dependence varied. Spring abundance was temporally stable in all regions, although harvest rates tended to decline, and adult survival rates tended to increase. Evidence for cyclical patterns in abundance was weak except in the Northeast, but this may be an artifact of the relatively short time series. Sustainable harvest potential varied amongst regions and was greatest in the East and Northeast. It appears that harvests during 2005–2022 were somewhat less than the sustainable maximum. Non‐equilibrium harvest strategies, in which the allowable harvests depend on spring population size and anticipated productivity, are so‐called ‘bang‐bang’ strategies, meaning that no or very little harvest is optimal until the population is at or above its maximum level of net production. Practical implications. Regional differences in ptarmigan demography warrant different harvest strategies. Yet, the hunting season in Iceland historically has been regulated on a country‐wide basis, with population abundance and dynamics in the Northeast playing a key role. To implement regional harvest management, decision makers must first agree on harvest management objectives that satisfy most stakeholders, who tend to hold very diverse values. Moreover, the historical relationship between season length and harvest rate is tenuous, yet season length is the primary mechanism traditionally used in Iceland to control harvest. The uncertainty in this relationship could be addressed in an adaptive‐management framework.

A non‐invasive genetic approach for estimating the apparent survival rate of a long‐lived territorial raptor: a case study on the Golden Eagle Aquila chrysaetos

For long‐lived species, adult survival is the parameter that theoretically has the strongest impact on population dynamics; this makes its estimate crucial for conservation management. The use of non‐invasive genetic approaches coupled with capture–recapture methods is being used increasingly to estimate adult survival. Despite being regularly employed in mammals, it has been little‐used in birds and notably in long‐lived territorial species. This study aimed to determine if feathers obtained from eagle nests could be used to estimate the adult apparent survival rate and the costs associated with such an approach. Feather samples (n = 180) were collected in nests (n = 78) and directly from individual Golden Eagles (n = 36) Aquila chrysaetos in France and were genotyped using 17 microsatellite markers. The genotyping error rates obtained were low, with 3% amplification failures, 3.1% dropouts and 1.7% false alleles. The genetic variability of the markers was high, with a probability of identity between siblings of 6.8 × 10−6, allowing reliable individual identification. Of the feathers collected in nests, 90% were from breeding females; this allowed apparent survival to be estimated for adult females at a reasonable cost. The genotyping of three feathers collected from a nest ensured individual identification of the breeding female with near certainty. Our simulations showed that monitoring of at least 20 pairs over 5 years, or 10 pairs over 10 years is necessary to detect a 10% absolute decrease in adult apparent survival. Estimating the adult survival rate of long‐lived birds using feathers collected in nests is possible. A substantial budget, albeit one that is likely to be lower than that for a common capture–recapture survey, would be needed to obtain precise survival estimates.

Age truncation due to disease shrinks metapopulation viability for amphibians.

Metapopulations often exist in a fragile balance between local extinctions and (re)colonisations, in which case emerging threats that alter species vital rates may drastically increase metapopulation extinction risk. We combined empirical data with metapopulation simulations to examine how demographic shifts associated with amphibian chytrid fungus (Batrachochytrium dendrobatidis, Bd) have altered metapopulation viability for threatened amphibians in Australia. Comparing the ages of museum specimens collected before Bd emerged in Australia with individuals from geographically matched remnant populations revealed significant truncation of age structures post-Bd, with a halving of annual adult survival probabilities. Spatially realistic metapopulation modelling demonstrated that reduced adult survival led to major reductions in the parameter space over which persistence was possible for the focal species, with contractions to landscapes with higher landscape connectivity, lower environmental stochasticity and considerably higher recruitment rates. Metapopulation persistence post-Bd required greater landscape connectivity than pre-Bd. This arises from a landscape-level analogue of compensatory recruitment at the population level, in which higher (re)colonisation rates can offset more frequent local extinctions, enabling persistence of amphibians susceptible to Bd. Interactions between recruitment rate, environmental stochasticity and landscape connectivity were also more important for metapopulation persistence post-Bd. Higher recruitment was required to mitigate the impacts of environmental stochasticity, and higher landscape connectivity was required to mitigate the impacts of environmental stochasticity and poor recruitment. Increased reliance on these interdependencies shrunk the parameter space over which metapopulations could persist post-Bd. Our study demonstrates that emerging threats that alter species vital rates can drastically reduce the capacity of certain environments to support metapopulations. For our focal species, reductions in adult survival rates due to Bd produced major reductions in the conditions under which persistence was possible, providing a mechanistic insight into the processes underpinning observed range and niche contractions of amphibians impacted by this pathogen. More broadly, our study illustrates how environmentally mediated host resilience can enable persistence following the emergence of novel pathogens. This pathway to persistence is worthy of greater attention on both conceptual and applied grounds.

Immunophenotypic Characteristics and Cytogenetic Analysis of Adolescent and Young Adult B-Cell Acute Lymphoblastic Leukemia: Correlations With Clinicopathological Parameters.

Introduction Acute lymphoblastic leukemia (ALL) has suboptimal survival rates for adolescents and young adults (AYA) as compared to children. Very limited studies have been conducted on AYA patients in India. This study aimed to identify the cytogenetic and immunophenotype characteristics of B-cell ALL (B-ALL) in AYA patients and determine its correlation with clinicopathological parameters in the Southern India region. Method The study was a prospective study conducted for three years, from June 2019 to May 2022, in India. Newly diagnosed 90 patients with AYA (13-40 years) ALL were recruited. A B-ALL diagnosis was made based on morphology with cytochemical stains and immunophenotype by flow cytometry (FCM). Cytogenetic analysis was also performed using karyotyping and fluorescent in situ hybridization to identify chromosomal aberrations. The cytogenetics results were correlated with immunophenotyping and clinicopathological characteristics. Variables were analyzed using the Mann-Whitney U test and Chi-square test using IBM SPSS Statistics for Windows, Version 20.0 (Released 2011; IBM Corp., Armonk, NY, USA). Results The mean age was 22.68 ± 8.06 years. It was observed that the most common structural chromosomal abnormality for AYA was t(9;22) in 14 (15%) cases, followed by 6q deletions in seven (8%) cases, t(1;19) in four (4%) cases, and t(12;17) and t(6;14) in two (2%) cases each. In addition, t(3;12), t(2;11), t(12;21), t(1;9), t(2;12), and t(X;10) were found in one (1%) case each. The most common numerical abnormality was hyperdiploidy (15; 17%), followed by hypodiploidy (10; 11%). Further, myeloid antigen expression of CD33 was the most common aberrantly expressed marker found in 20 (28%) cases, followed by CD15 in three cases (5%), CD13 in three (4%) cases, and CD11b in two (3%) cases. It was also observed that in Ph+ve cases, CD33 and CD13 were most commonly expressed in three (33%) and two (17%) cases, respectively. In contrast, in Ph-ve patients, their expressions were lesser at 17 (27%) and one (2%) cases, respectively. In addition, leukemia-associated immunophenotype pattern (LAIP) markers CD44 6 (86%) and CD123 5 (55%) were also found to be significantly associated with Ph+ve, whereas their values in the Ph-ve group were lesser at 25 (42%) and 9 (17%), respectively. Our data also showed that older age wassignificantly associated with Ph+ve with a median age of 30 years (p = 0.012). In comparison, the median age of Ph-ve was only 21 years. Conclusion Our study established that the incidence of cytogenetic abnormalities for AYA was consistent with previously reported data. This study reaffirms that Ph+ve cases have significant associations with MyAg (CD13), LAIP (CD123 and CD44), and older age for the South Indian population.

Evaluation of potential factors affecting the success of Cisco re-introduction and re-establishment in Lake Huron

Recent food web changes led to an opportunity to reintroduce (fingerling stocking) Cisco in central Lake Huron. We examined 9 factors affecting the ability of stocked Cisco fingerlings to survive, and reproduce and for their progeny to survive and reproduce. These values (obtained from the literature) were applied to a stochastic Leslie Matrix model and projected forward 30 years. The mean terminal eigenvalue (λ) of the final 10 years was used to indicate if the resulting wild population would continue to increase or decrease and thresholds for each variable. Recovery success was sensitive to survival rates especially in early life stages. Numbers stocked and the rate of homing by hatchery fish only affected the rate of recovery. Adult survival, if adjusted up, helped compensate for many of the earlier life stages lower survival rates. Simulations indicated the likelihood of successful establishment of ongoing natural reproduction if periodicity of boom years of reproduction were at least once every 5 years on average, but higher adult survival rates can relax this requirement allowing for an expanding population at less frequent periodicities.

Effect of ultraviolet on the environmental adaptability of Megalurothrips usitatus (Thysanoptera: Thripidae).

Megalurothrips usitatus Bagnall, an important pest of bean plants, is primarily managed with synthetic insecticides. M. usitatus has developed considerable resistance to various insecticides in multiple cowpea-growing areas in Hainan Province, China, posing challenges to its control in the field. Light control technology is a potentially effective physical control method for M. usitatus. The vision of thrips is highly sensitive to UV light, whereas other biological characteristics remain unknown. Therefore, this study evaluated the effects of ultraviolet light on the biological characteristics of M. usitatus. Results showed that the egg, larval, and pupal stages of M. usitatus were significantly shortened, and the emergence rate (79.59%) and adult survival rate (77.95%) were reduced under a devoid of UV light environment (UV-), compared with the full-spectrum light (control treatment group, CK) (p < 0.05). However, the single spawning quantity and total amount of spawning were significantly higher, and the sex ratio (57%) was the highest under UV- (p < 0.05). Single UV light (UV+) only affected the pupation rate. Also, the antioxidant enzymes, polyphenol oxidase, superoxide dismutase (SOD), and peroxidase activities were significantly and negatively correlated with the progression of generations under UV-, whereas catalase and SOD activities were significantly and positively correlated with the progression of generations under UV+. The UV- light conditions significantly interfered with the behavior selection of M. usitatus. The results of this study showed that the adaptability of M. usitatus populations would be greatly reduced in the absence of ultraviolet light, providing a theoretical basis for the control of M. usitatus populations.

Cold tolerance and metabolism of red-haired pine bark beetle Hylurgus ligniperda (Coleoptera: Curculionidae) during the overwintering period.

Hylurgus ligniperda invaded Shandong, China, through imported forest timber, posing a threat to China's forest health. Exotic insects with broad environmental tolerance, including low temperatures, may have a better chance of surviving the winters and becoming invasive. Understanding the cold-tolerance strategies of H. ligniperda may help to design sustainable pest management approaches. In this study, we aim to investigate the cold-tolerance ability and relevant physiological indicators in overwintering H. ligniperda adults to determine any possible overwintering strategies. Supercooling points (SCPs) for adults H. ligniperda differed significantly across months and reached the lowest level in the mid- and post-overwintering period, the minimum SCPs -6.45 ± 0.18 °C. As the cold exposure temperature decreased, the survival rate of adults gradually decreased, and no adult survived more than 1 day at -15 °C, and the LLT50 for 1 day was -7.1 °C. Since H. ligniperda adults can survive internal ice formation, they are freeze-tolerant insects. Throughout the overwintering period, the SCPs and the water, protein, sorbitol, and glycerol content in adults decreased initially and then increased. We reported significant correlations between total protein, sorbitol, trehalose, and glycerol content in the beetles and SCPs. Glycogen, lipid, protein, trehalose, and sorbitol content in adult beetles may directly affect their cold-tolerance capacity and survival during winter. This study provides a physiological and biochemical basis for further study of metabolism and cold-tolerance strategies in H. ligniperda adults, which may help predict population dynamics and distribution potential of pests.