Survival Rate Of Catfish Research Articles

Utilization of palm oil mill effluent as growth media in budikdamber system

Palm oil mill effuent (POME) has the potential to become an environmental pollutant if left unutilized. This study aims to examine the effect of POME on catfish and water spinach growth in the bucket fish culture system (budikdamber). This research was conducted in August-November 2021, and comprises of acclimatization of fish, seeding, POME media preparation, rearing, observation, and data analysis. This study used a completely randomized design, consisting of four treatments and three replications. The treatments was the POME concentrations, consisting of Control (0 mg.L−1), A (1.565 mg.L−1), B (2.347 mg.L−1), and C (3.130 mg.L−1). The fish used were catfish measuring 5-7 cm totaling 20 fish/bucket, while the plant used was seedlings measuring 2-3 cm. Data were analyzed using one-way ANOVA at 95% confidence level. The research parameters included the catfish Specific Growth Rate (SGR) and Survival Rate (SR), along with the height, number of branches, and leaves of water spinach. The results showed that the concentration of 3.130 mg.L−1 caused significant differences in fish and plant growth parameters. SGR of catfish decreased significantly at concentrations of 2.347 and 3.130 mg.L−1, meanwhile, SR of catfish was not significantly different. Concentrations of 1.565, 2.347, and 3.130 mg.L−1 showed a significant increase in height, branches, and leaves number of water spinach plants.

EFEKTIVIAS EKOENZIM TERHADAP KELULUSHIDUPAN IKAN LELE (Clarias sp.) PADA MEDIA YANG TERCEMAR LIMBAH BATIK

Ecoenzymes are active substances that can accelerate the degradation process of waste and improve the quality of the environment. The benefits of ecoenzymes for the environment, particularly in agriculture as fertilizer, and in households as a germ and bacteria killer are very good. It is hoped that ecoenzymes can neutralize batik waste, which is toxic to aquatic environments. This study aims to evaluate the effectiveness of ecoenzymes on the survival rate of catfish (Clarias sp.) in media contaminated with batik waste. The method used is an experiment using three concentrations of ecoenzymes and measuring the sustainability of catfish life at each concentration. Based on the results of the research, catfish that were exposed to batik waste and added with ecoenzymes with concentrations of 0% and 10% showed that the catfish could not survive. This shows that ecoenzymes have not yet been proven to increase the effectiveness in neutralizing batik waste against the survival rate of catfish (Clarias sp.).

THE GROWTH AND SURVIVAL RATE OF CATFISH (Clarias gariepinus) REARED INTENSIVELY USE BIO-FLOC TECHNOLOGY

The study purposed to investigate the growth and survival rate of catfish (Clarias gariepinus) reared intensively use bio-floc technology. The research used an experimental method, consists of two treatments. Treatment A was catfish reared uses Noni fruit (Morinda citrifolia) fermentation on culture media every seven days with a dose of 10 ml/liter. The treatment also uses papaya leaves as additional feed. Treatment B was catfish reared apply bio-floc technology use probiotics. Probiotic consists of Lactobacillus spp. and Thiobacillus spp. The addition of 5 grams of Lactobacillus spp. and 500 ml molasses in rearing media were carried out every seven days. The feed used in the experiment was fermented by Thiobacillus spp. and molasses at a dose of 150 ml/kg of feed. Two plastic tanks with a volume of 5000 L were used in the experiments. Amount 3000 catfish with the average initial body weight of 6 grams reared in each plastic tank. Catfish were fed twice daily with a feeding rate of 7%. Feed nutrition content was protein minimum 28%, fat maximum 4%, and fiber maximum 5%. Catfish were reared for 64 days. The research data consist of fish growth, feed conversion ratio, and survival rate of fish. Data were analyzed quantitatively descriptively. The results showed that survival rate of catfish in treatment B higher than treatment A, but growth of catfish in treatment B lower than treatment A. Feed conversion ratio in treatment A lower than treatment B. The specific growth rate, feed conversion ratio, and survival rate of catfish in treatment A were 5.19%, 69.83%, and 0.78, respectively. At the same time, the specific growth rate, feed conversion ratio, and survival rate of catfish in treatment B were 3.89%, 90.80%, and 0.95, respectively. The results were indicated that bio-floc technology could improve the survival rate of catfish but no increased growth of catfish.

Effect of probiotics addition on total organic matter and survival rate of catfish (Clarias sp.) maintenance using recirculating aquaculture system (RAS)

Most catfish farmers do not make efforts to manage water quality. This causes high organic matter. Therefore, water management is carried out through the Recirculating Aquaculture System (RAS). Continuous use of water in the RAS system causes filter performance to decrease, so technological improvements are needed with the addition of probiotics. The research was conducted on 22 February – 23 March 2021 at the Chemistry and Analysis Laboratory, Faculty of Fisheries and Marine, Universitas Airlangga to determine the influence of probiotic additions on the RAS system total organic matter and the survival rate of catfish.The study was conducted experimentally using RAL by adding different doses of probiotics, namely P0 (Control), P1 (0.5 ml/L), P2 (1 ml/L), P3 (1.5 ml/L) and P4 (2 ml/L ) with four replays. Data analysis using ANOVA and DMRT showed that the addition of probiotics in the RAS system has an effect (p<0.05) on total organic matter and survival rate. Organic matter was highest at P2 (46.89 mg/L) and lowest at P2 (34.07 mg/L). Survival rates were highest at P2 (85%) and lowest at P0 (71.25%).

Fermented medicinal herbs improve hematological and physiological profile of Striped catfish (Pangasianodon hypophthalmus)

This study sought to determine the effect of fermented medicinal herbs (FMH), i.e. cutchery (Kaempferia galanga), turmeric (Curcuma longa) and curcuma (Curcuma xanthorrhiza) in combination with molasses and probiotic drink (Yakult), administered orally on the hematological and physiological profile of striped catfish (Pangasianodon hypophthalmus). A complete randomized design (CRD) experiment was used with four levels of treatments, namely P0 (control), P1 (FMH 100 mL/kg), P2 (FMH 200 mL/kg) and P3 (FMH 300 mL/kg) of feed. The fish were kept in a farm in cages at 75 fish/m3 and fed with the experimental diets for 60 days. The results revealed that FMH (P2) dietary administration improved hematological and physiological profile of catfish, i.e total erythrocytes of 2.81 x 106 cells/mm3, hematocrit values of 39.00%, hemoglobin levels of 10.73 g/dL, total leukocytes of 11.41 x 104 cells/mm3, blood glucose 97.33 mg/dL, and total serum protein 4.10 mg/dL compared to controls with 1.89 x 106 cells/mm3, 32.33 %, g/dL, 9.67 x 104 cells/mm3, 67.33 mg/dL, and total serum protein of 3.10 mg/dL, respectively. Moreover, the diet improved special growth rate, feed conversion ratio, feed efficiency and the survival rate of catfish. The hematological and physiological profile of catfish improvement are considered to be due to the content of secondary metabolites of FMH, namely curcuminoids, vitamin C, essential oils, tannins, and flavonoids, which trigger immunostimulation. The presence of curcuminoids provide an antioxidant effect on cell membranes reducing erythrocyte cell membrane damage due to oxidation. Similarly, flavonoids are natural antioxidants, which are credited with the ability of reducing free radicals and anti-free radicals.

Application of Carbon and Nitrogen Ratio in The Sangkuriang Catfish Culture Clarias gariepinus var in Tarpaulin Tanks

Sangkuriang catfish (Clarias gariepinus var) is a freshwater commodity fish that has the potential to be developed. Sangkuriang catfish farming activities still often experience problems in their implementation, especially water quality problems in the form of a buildup of organic matter that does not decompose and causes poison to catfish. The determining factor of success in growing heterotrophic bacteria in aquaculture waters is the ratio of C/N in the tank of sangkuring catfish. This study aims to determine the C/N ratio, growth rate, and survival of catfish. The results showed that the addition of a C/N ratio of 10:1 has an effect on the growth, survival rate of catfish and can reduce the ammonia content in catfish culture media.

Fermented medicinal herbs improve hematological and physiological profile of Striped catfish (Pangasianodon hypophthalmus)

This study sought to determine the effect of fermented medicinal herbs (FMH), i.e. cutchery (Kaempferia galanga), turmeric (Curcuma longa) and curcuma (Curcuma xanthorrhiza) in combination with molasses and probiotic drink (Yakult), administered orally on the hematological and physiological profile of striped catfish (Pangasianodon hypophthalmus). A complete randomized design (CRD) experiment was used with four levels of treatments, namely P0 (control), P1 (FMH 100 mL/kg), P2 (FMH 200 mL/kg) and P3 (FMH 300 mL/kg) of feed. The fish were kept in a farm in cages at 75 fish/m3 and fed with the experimental diets for 60 days. The results revealed that FMH (P2) dietary administration improved hematological and physiological profile of catfish, i.e total erythrocytes of 2.81 x 106 cells/mm3, hematocrit values of 39.00%, hemoglobin levels of 10.73 g/dL, total leukocytes of 11.41 x 104 cells/mm3, blood glucose 97.33 mg/dL, and total serum protein 4.10 mg/dL compared to controls with 1.89 x 106 cells/mm3, 32.33 %, g/dL, 9.67 x 104 cells/mm3, 67.33 mg/dL, and total serum protein of 3.10 mg/dL, respectively. Moreover, the diet improved special growth rate, feed conversion ratio, feed efficiency and the survival rate of catfish. The hematological and physiological profile of catfish improvement are considered to be due to the content of secondary metabolites of FMH, namely curcuminoids, vitamin C, essential oils, tannins, and flavonoids, which trigger immunostimulation. The presence of curcuminoids provide an antioxidant effect on cell membranes reducing erythrocyte cell membrane damage due to oxidation. Similarly, flavonoids are natural antioxidants, which are credited with the ability of reducing free radicals and anti-free radicals.

Growth Performance and Survival Rate of Catfish (Pangasius sp) with the Application of the Nitrifying and Denitrifying Bacteria

Nitrifying and denitrifying bacteria can be used as a bioremediation agents in aquaculture. The purpose of this experiment is to evaluate the optimal growth and survival performances of catfish rearing with the application of nitrifying and denitrifying bacteria. A completely randomized design was performed with the following treatments: A) nitrifying and denitrifying bacteria NP2-DP1; B) nitrifying and denitrifying bacteria NP2-DP2; C) commercial bacteria and D) without bacterial isolate (control), each with 3 replications. Twelve containers (34 x 34 x 45 cm) were used with a volume of 20 L equipped with aeration. The catfish used (Pangasius sp) has a body weight of 8.33 g ± 0.1 and stocking density of 20 fish / container reared within 30 days. Feed was applied to the fish at 3% of their body weight for three times a day at 08.00 am, 12.00 and 15.00 pm . . Inoculation of bacteria on day 10th and; 20th, that is 108 cfu / mL. The parameters measured were growth rate, survival rate, and water quality. Sampling was carried out every 10 days. The results showed that the application of NP2 and DP1 was the optimal to increase the growth and survival of catfish.

Utilization of herbal supplements on feed on growth and survival rate of catfish (Clarias sp.)

The feed is one of the most important factors in the growth and survival of fish. One of the efforts made to increase the growth and survival of fish is the provision of herbal supplements in fish feed. The research aim is to provide information about the utilization of herbal supplements on the growth and survival of catfish (Clarias sp). This research has been carried out from July to August 2020 at Bandar Agung Lahat Village, South Sumatera. Catfish rearing containers use two waring units placed in concrete ponds with two treatment that P0: control (without herbal supplements) and P1: utilization of herbal supplements and. The data taken includes absolute growth and absolute length, survival, feed efficiency, and water quality. The utilization of herbal supplements has a good effect on fish growth, feed efficiency, and survival rate. Maintenance of P0 (control) resulted in an absolute weight of 9.79 grams, an absolute length of 6.3 cm, the survival of 85.15%, feed efficiency of 107.89% while maintenance of P1 with the utilization of herbal supplements resulted in an absolute weight of 14.17 grams, an absolute length 7.8 cm, survival 98.57%, feed efficiency 161.170%.

Fermented medicinal herbs improve hematological and physiological profile of Striped catfish (Pangasi anodon hypophthalmus)

This study sought to determine the effect of fermented medicinal herbs (FMH), i.e. cutchery ( Kaempferia galanga), turmeric ( Curcuma longa) and curcuma ( Curcuma xanthorrhiza) in combination with molasses and probiotic drink (Yakult), administered orally on the hematological and physiological profile of striped catfish ( Pangasi a nodon hypophthalmus). A complete randomized design (CRD) experiment was used with four levels of treatments, namely P0 (control), P1 (FMH 100 mL/kg), P2 (FMH 200 mL/kg) and P3 (FMH 300 mL/kg) of feed. The fish were kept in a farm in cages at 75 fish/m 3 and fed with the experimental diets for 60 days. The results revealed that FMH (P2) dietary administration improved hematological and physiological profile of catfish, i.e total erythrocytes of 2.81 x 10 6 cells/mm 3, hematocrit values of 39.00%, hemoglobin levels of 10.73 g/dL, total leukocytes of 11.41 x 10 4 cells/mm 3, blood glucose 97.33 mg/dL, and total serum protein 4.10 mg/dL compared to controls with 1.89 x 10 6 cells/mm 3, 32.33 %, g/dL, 9.67 x 10 4 cells/mm 3, 67.33 mg/dL, and total serum protein of 3.10 mg/dL, respectively. Moreover, the diet improved special growth rate, feed conversion ratio, feed efficiency and the survival rate of catfish. The hematological and physiological profile of catfish improvement are considered to be due to the content of secondary metabolites of FMH, namely curcuminoids, vitamin C, essential oils, tannins, and flavonoids, which trigger immunostimulation. The presence of curcuminoids provide an antioxidant effect on cell membranes reducing erythrocyte cell membrane damage due to oxidation. Similarly, flavonoids are natural antioxidants, which are credited with the ability of reducing free radicals and anti-free radicals.

INTEGRASI BUDIDAYA IKAN PATIN (Pangasius hypophthalmus) DAN TANAMAN AIR PADA PEMELIHARAAN SISTEM AKUAPONIK

The purpose of this study is to find out whether the integration of catfish cultivation (P. hypophthalmus) and aquatic plants in the maintenance of aquaponic systems in order to produce high fish survival. The research was conducted using a Complete RandomIzed Design (RAL) environmental design with 4 (four) treatments and 3 (three) replays, each of these treatments is :P A behavior: 10 plant stems / holes and 2 fish / liter, Treatment B : 10 plant stems / holes and 3 fish / liters, Treatment C : 10 plant stems / holes and 4 fish / liter, Treatment D : 10 plant stems / holes and 5 fish / liter. The observed parameters are survival, growth and measurement of water quality. The results showed that the survival rate of catfish was 85.18%, 2.38 cm for length gain and weight gain of 2.80 g

Fermented medicinal herbs improve the hematological and physiological profile of Striped catfish (Pangasionodon hypophthalmus)

This study sought to determine the effect of fermented medicinal herbs (FMH), i.e. cutchery ( Kaempferia galanga), tumeric ( Curcuma longa) and curcuma ( Curcuma xanthorrhiza) in combination with molasses and probiotic drink (Yakult), administered orally on the hematological and physiological profile of striped catfish ( Pangasionodon hypophthalmus). A complete randomized design (CRD) experiment was used with four levels of treatments, namely P0 (control), P1 (FMH 100 ml/kg), P2 (FMH 200 ml/kg) and P3 (FMH 300 ml/kg) of feed. The fish were kept in a farm in cages at 75 fish/m 3 , and fed with the experimental diets for 60 days. The results revealed that FMH (P2) dietary administration improved hematological and physiological profile of catfish, i.e total erythrocytes of 2.81 x 10 6 cells/mm 3, hematocrit values of 39.00 %, hemoglobin levels of 10.73 g/dl, total leukocytes of 11.41 x 10 4 cells/mm 3, blood glucose 97.33 mg/dl, and total serum protein 4.10 mg/dl compared to controls with 1.89 x 10 6 cells/mm 3, 32.33 %, g/dl, 9.67 x 10 4 cells/mm 3, 67.33 mg/dl, and total serum protein of 3.10 mg/dl, respectively. Moreover, the diet improved special growth rate, feed conversion ratio, feed efficiency and the survival rate of catfish.

Growth performance and immunological response of African Catfish (Clarias gariepinus) juveniles reared in biofloc system

This study was conducted to examine the growth and immune performance of catfish ( Clarias gariepinus ) juveniles cultured in a biofloc fish farming system. To achieve this, 900 C. gariepinus juveniles (9.0 ± 0.23 g) were cultured in an intensive zero water exchange biofloc system for 72 days in 9 separate tanks (2 m 3 each) aerated by an air blower, after which growth assessment of fish was conducted. The trial consisted of three treatments in triplicates, a control without carbon source addition, and two biofloc treatments with carbon source addition of rice bran or cassava flour. The two carbon sources added had a C/N rate of 15:1 to form the floc. The results showed no significant difference between dissolved oxygen (DO), pH, and temperature in all the treatments, however, conductivity, total dissolved solids, and salinity showed significant differences between the bioflocs and control treatments and the treatment group. Nevertheless, the obtained values for the water parameters were within the range required for culturing C. gariepinus. Survival rate of catfish was significantly higher in the biofloc culture with a cassava carbon source (98.3%) compared with the control experiment (64.3 %). Weight gain of the fish was highest in rice bran based biofloc (44.9 ± 3.00 g) and lowest (37.0 ± 4.15 g) in the control treatment. The non-specific immune assay revealed that monocytes, serum lysozyme, and myeloperoxidase were higher in the biofloc treated groups compared to the control group. In contrast, neutrophils' percentage was lower in the treated groups than in the control group. Therefore, this study demonstrated the suitability of biofloc as an aquaculture wastewater purifier, growth-promoting, and immune-enhancing technology for the small-scale culture of C . gariepinus .

The Effect of Adding Bacillus NP5 to Feed on Growth, Survival Rate, and Protection Against Aeromonas hydrophila of Catfish (Clarias sp.)

Probiotics are useful organisms in preventing the spread of disease in aquaculture. Therefore this study aims to evaluate the effect of administering Bacillus NP5 on the growth, survival rate, and histology of catfish with Aeromonas hydrophila infection. To achieve this, catfish (7.75 ± 0.02 g) were reared for 45 days with a density of 20 per tank. Furthermore, the study consisted of 4 treatments and 3 replications, namely K+: 0% Bacillus NP5+0.1 mL A. hydrophila injection, K-: 0% Bacillus NP5 + without A. hydrophila injection, A: 0.3% Bacillus NP5, and B: 0.8% Bacillus NP5. The results showed that the addition of probiotics significantly increased the growth and survival rate of catfish (P <0.05) compared to K+. The higher value of specific growth rate was found in treatments A and B (0.22 ± 0.03 and 0.23 ± 0.03% day-1, respectively) and the lowest in K+ (0.14 ± 0.03% day-1). Also, the tissue damage in K+ was higher than in the probiotic treatment. Besides, hyperplasia, congestion, and secondary lamella fusion in the gill tissue were found in the K+, while other treatments were found only in hyperplasia. Melanomacrophages occurred in catfish kidney tissue for all treatments, however, hydropic degeneration was found in the control treatment. The addition of Bacillus NP5 in feed resulted in higher growth, survival, and tissue damage compared to K+. Also, the addition of 0.8% Bacillus NP5 resulted in less catfish tissue damage.

ANALYSIS OF DIFFERENT C:N RATIOS IN THE BIOFLOC SYSTEM ON THE GROWTH OF Pangasius hypophthalmus (Sauvage, 1878)

The development of catfish culture affects increasing waste in the waters. Aquaculture waste containing high nutrients has the potential to damage the cultivation environment. The Biofloc system in catfish farming can break down NH3 waste into flocks that can be consumed directly by fish. This study aims to analyze the growth and survival rate of catfish in biofloc systems with different C:N ratios. The research design used was a completely randomized design (CRD) with four treatments and three replications. Siamese catfish seeds were maintained in an aquarium container size 40 x 30 x 30 cm for 40 days with treatment media A (maintenance without biofloc), treatment B C:N ratio 15, treatment C C:N ratio 20, and treatment D C:N ratio 25. The results showed that the biofloc system with different C:N ratios had significant effect on the weight growth, daily growth rate, and feed convertion ratio of catfish. The best treatment for growth and viability of catfish is C:N ratio 25.

The growth performance and survival rate of catfish (Clarias sp.) that given probiotic controlling nitrogen

The water quality can be improved by using probiotics as bioremediation. The probiotics are a consortium of nitrification and denitrification bacteria (Pseudomonas aeruginosa and Achromobacter insuavis), which both of the indigenous bacteria are the products of the Research Institute for Freshwater Aquaculture and Fisheries Extension (RIFA). The objective study is to determine the adding frequency of RIFA probiotic into the water fish pond of catfish culture concerning growth performance and survival. The catfish fingerling with a size of 7-8 cm long and weighing 16.45 ± 0.3 g was used. The stocking density was 750 fish/pond. Nine plastic sheet ponds with a water volume of 1.6 m3 and completed with aeration were used. A dose of probiotics was 10 mL/m3. The difference in adding the frequency of probiotic as a treatment was as followed: A) every 5 days; B) every 10 days, and C) without probiotics. The parameters measured survival rate (SR), absolute weight growth, specific growth rate (SGR), feed conversion ratio (FCR), water quality. One way ANOVA with three treatments and three replications were performed. The results showed that the use of probiotics could control nitrogen compounds in catfish cultivation, where the frequency of administration every 5 days gives the best results.

Application of organic fertilizer on swamps pond for catfish (Pangasius sp.) culture

Swamp fish pond has a problems related to submerged flat soil and water pH value and result in low productivity of ponds. Fertilization increase productivity of pond water by providing nutrients for fish food organisms, The objective of this research was to determine the best dosage of organic fertilizer to improve water quality and pond productivity and it’s effect to survival and absolute growth of catfish Pangasius sp. The research was conducted with using completely randomized design with four treatments and three replications. All of ponds unit were limed using dolomite of 10 ton/ha, incubated for 13 days, then gave four difference dosage of organic fertilizer, that were without fertilizer (P0), with fertilizer of 100 g/m2 (P1), 170 g/m2 (P2), and 240 g/m2 (P3). Pangasius sp. fish (average body length of 5±0.5 cm) was stocked with the density of 15 fish/m2 and cultured for 30 days. The parameters observed were soil (pH, N, P2O5, Ca, and C-organic) and water quality (pH, alkalinity, temperature, dissolved oxygen, ammonia, total Phosphate, Ca, and TOC), pond productivity, density of phytoplankton, survival, absolute growth, feed efficiency, and production of fish. The data were statistically analyzed by one-way ANOVA, Least Significant Difference test, and regression models analysis. The results showed that P1 (100 g/m2 of fertilizer) was the best dosage indicated by pH 7.60, nitrogen 0.13%, P2O5 0.16%, Ca 2.59%, C-organic 1.49% of pond soil; pH 7.42, temperature 27.3-32.4°C, dissolved oxygen 4.94-5.09 mgL−1, alkalinity 51.33 mgL−1, Ca 55 mgL−1,TOC 12.3 mgL−1, ammonia 0.24 mgL−1, Total Nitrogen (TN) 3.78 mgL−1, Total Phosphate (TP) 0.109 mgL−1 of pond water. Based on TN:TP (10.09) of water showed nutrient was balanced. Productivity of ponds was on eutrophic state based on both TN and TP. Survival rate of catfish was 100%, absolute gain of weight 8.46 g and lenght 3.99 cm, Feed efficiency of fish was 101.55% and the density of phytoplankton was 217 cell/mL.

Efektivitas Infusum Daun Durian (Durio zibethinus) Sebagai Anestesi Alami Ikan Lele (Clarias gariepinus)

Bahan anestesi buatan atau alami sangat dibutuhkan dalam transportasi ikan hidup, karena ikan secara fisiologi harus tetap hidup dan sehat sampai tempat tujuan. Daun durian adalah merupakan salah satu bahan alami yang dapat digunakan sebagai anetesi dalam transport karena mengandung mengandung senyawa metabolit sekunder seperti saponin, tanin dan flavonoid. Tujuan dari penelitian ini yaitu untuk mengetahui konsentrasi efektif infusum daun durian sebagai anestesi ikan lele terhadap waktu induksi dan sedatif, profil glukosa darah dan sintasan. Ikan dengan berat rataan 185,62 ± 9,06 g dalam 3 L air dengan konsentrasi bahan anestesi daun durian yang berbeda digunakan untuk mengetahui status pingsan ikan. Rancangan acak lengkap dengan empat perlakuan (konsentrasi infusum daun durian 5%, 15%, 25%, dan 35%.) dan tiga ulangan digunakan dalam penelitian ini. Hasil dari penelitian ini menunjukkan bahwa konsentrasi terbaik yang diperoleh adalah 35%, dengan waktu induktif 9 menit-19 detik dan waktu sedatif 1 menit-42 detik. Sintasan ikan lele pada perlakuan terbaik sebesar 88.89%, dengan kadar glukosa darah sebesar 63,23 mg/dL.

The Effect of Papain Enzymes and Probiotic Addition on Feed Towards The Catfish Growth Performance, Feed Utilization Efficiency and Survival Rate in Biofloc Techniques

The success of catfish farming (Clarias sp.) is influenced by feed and water quality. The low feed utilization results in high feed production costs. Alternative use of feed using the enzyme papain and probiotics, the papain enzyme is able to hydrolyze proteins into peptides to increase the utilization of protein in feed by the body. In addition, increased digestibility is done by the addition of probiotic bacteria such as Lactobacillus sp., Saccharomyses sp., Azotobacter sp., Streptomyces sp., Aspergillus sp., Trichoderma sp. into food that can increase feed digestibility and nutrient absorption. Water quality is one of the factors that caused a low survival rate of fish. Biofloc technology utilizes microbial activity that can convert organic waste intensively to form a flock that used by fish as a food source. This study aims to examine the effect of papain and probiotics enzyme addition in feed towards the growth, efficiency of feed utilization and survival rate of catfish using biofloc techniques. The research used a Completely Randomized Design (CRD) by five treatments using biofloc maintenance media and five treatments using non-biofloc, each repeated three times. This research used catfish with a density of 1 head /L kept for 4 weeks as a test animal, treatment P0 (test feed 0.0 gr / kg papain enzyme and 0 mL probiotics), P1 (test feed 0.25 gr / kg papain enzyme and 1 mL probiotic), P2 (Test feed 0.25 gr / kg papain enzyme and 2 mL probiotics), P3 (Test feed 0.5 gr / kg papain enzyme and 1 mL probiotic), P4 (Test feed 0.5 gr / kg of papain enzyme and 2 mL probiotics). The results showed that the addition of the papain and probiotics enzyme in feed with the best dosage was P2 with biofloc maintenance media that gave a real effect (P &lt;0.05) on the efficiency of feed utilization and survival, but had no significant effect (P&gt; 0.05) on growth of catfish (Clarias sp.). Water quality in the maintenance media is in a decent range for the maintenance of test fish.

The potential of mixed epibiotic (binahong leaves, anrederacordifolia, and garlic, allium sativum, extracts) as a feed additive to combat aeromonashydrophilainfection on catfish (clariasgariepinus)

Catfish are one of the most commercially important fish cultured and consumed in Indonesia. Catfish (Clarias garipienus) is one of the most commercially important freshwater fish cultured and consumed in Indonesia. However, Motile Aeromonas Septicemia (MAS) disease was caused by Aeromonas hydrophila is one of the obstacles which are often to be found during the culture. This study aimed was to determine the epibiotic extracts of binahong leaves (BLE) and garlic (GE) as a feed additive on the growth performance and survival rate of catfish against Aeromonas hydrophila infection. This research was conducted with Completely Randomized Design (6 treatments and 3 replications)in the aquaculture department laboratory, Fisheries and Marine Science Faculty of Diponegoro University. The treatments were various dosages of the mixture of epibiotic extracts, namely A (2.500 ppm BLE+0 ppm GE), B (1.875 ppm BLE+250 ppm GE), C (1.250 ppm BLE+500 ppm GE), D(625 ppm BLE+750 ppm GE), E (0 ppm BLE+1.000 ppm GE) and K (0 ppm BLE+ 0 ppm GE). The result of the research indicated that a mixture of epibiotic was able to increase the survival rate but not in the growth rate. This treatment also was affected by the blood profile of cat fish ie. Erythrocyte, leucocyte, and hemoglobin These results showed that the best dosage of a mixture of epibiotic extracts (1.250 BLE+500 ppm GE) resulted in 86,67% of survival rate.