BACKGROUND Although Omicron's hospitalization rate and mortality rate decreased significantly in the immunocompetent population (Wang B, et al. J Infect 2023; Maslo C, et al. JAMA 2022), it remains a fatal threat to patients with immunocompromised hematological malignancies (HM) (Zhu X, et al. BJH 2023). The overall mortality rate of Omicron infection was 16.5% among hospitalized HM patients according to the “EPICOVIDEHA survey report” (Blennow O, et al. AJH 2022), significantly higher than that observed in the general population. The main cause of Omicron-related death in HMs was respiratory failure caused by Omicron pneumonia and data showed that approximately 10% of HM patients develop Omicron pneumonia after infection (Zhu X, et al. BJH 2023). However, little is known about the risk factors for Omicron pneumonia in HM patients after Omicron infection. METHODS The data in this study comes from a registered multi-center, prospective, observational study during the latest Omicron wave in Chongqing, China (November 2022 to January 2023), of which the initial purpose was to investigate the neutralizing antibody levels in HM patients after Omicron infection. Detailed information can be found on international clinical trials registry platform (ICTRP) of world health organization (WHO) (https://trialsearch.who.int/) (No.ChiCTR2300071830) or the Chinese clinical trial registry website (http://www.chictr.org.cn). Patients were enrolled at the time of diagnosis of SARS-CoV-2 infection. Immune function stats, which was assessed by the counts of immune cells including neutrophils, total lymphocytes, CD4 (+) T cells, B cells, NK cells, were measured at the time of enrollment. All patients were followed up once a week for a total of 6 weeks after enrollment. During the period, chest CT scans were performed to determine Omicron pneumonia on patients with any of the following characteristics: 1) Sustained high fever > 3 days; 2) Respiratory rate ≥ 30 beats/minute; 3) Oxygen saturation at rest < 93%; 4) PaO 2/FiO 2 ≤ 300mmHg. The sequencing results were download from the Global Initiative on Sharing Avian Influenza Data (GISAID) database (https://db.cngb.org/gisaid) to identify the potential SARS-CoV-2 strains present in our patients. Risk factors were analysed by logistic univariate / multivariate regression methods. RESULTS Omicron subvariant BA.5.2.48 was speculated to be the dominant SARS-CoV-2 strain in our patients. A total of 475 HM patients enrolled in this study. Omicron pneumonia was observed in 15.8% (75/475) of patients. In the Omicron pneumonia group, patients had a median age of 58 years [IQR 48-69] and males accounting for 61.3%, 56 (74.7%) patients with a baseline disease of lymphoma, 41 (54.7%) with disease status of active disease, 33 (44.0%) patients had no COVID-19 vaccination history, and 65 (86.7%) patients had received targeted chemotherapy; B/ CD4 (+) T/ NK-cell reduction rate were 49 (65.3%), 66 (88.0%) and 47 (62.7%), respectively. Risk factors associated with Omicron pneumonia included active disease status of HM at infection (OR=3.42, 95% CI: 1.59-7.37, P=0.002), (1-2) dose of COVID-19 vaccination (OR=2.55, 95% CI: 1.28-5.10, P=0.008), no COVID-19 vaccination history (OR=4.81, 95% CI: 2.45-9.43, P<0.001), chemotherapy prior to infection <6 months (OR=2.58, 95% CI: 1.12-5.96, P=0.027), chemotherapy prior to infection ≥ 6 months (OR=2.93, 95% CI: 1.31-6.53, P=0.009) and NK-cell reduction (< 150/μL) (OR=2.19, 95% CI: 1.27-3.79, P=0.005). CONCLUSIONS Our study investigated risk factors for Omicron pneumonia in HM patients after Omicron (BA.5.2.48) infection. Highlights that HM patients with these risk factors, may be susceptible to lung involvement after Omicron infection and need to be taken seriously in clinical practice.