Background: Today, government agencies, including medical universities, are facing a changing environment that needs to take advantage of new approaches such as entrepreneurship and organizational agility to maintain or enhance competitive advantage. Therefore, this study was designed to identify the factors affecting entrepreneurship and agility of organizations that have a great impact on sustainability in today's changing and competitive environment of organizations. Methods: The present study is a qualitative study that was done in two stages of reviewing literature and expert opinion survey by Delphi method. In the first step, the variables affecting agility and organizational entrepreneurship were extracted by reviewing related literature. The variables extracted were then finalized by Delphi method and after reaching the consensus of experts. Based on the findings of the study, 8 dimensions for organizational agility questionnaire and 6 dimensions for entrepreneurship questionnaire were identified. For organizational agility, 27 components and 39 components for entrepreneurship were obtained. Results: The results showed that there are many different factors affecting entrepreneurship and agility of the organization, including factors affecting organizational agility, flexibility, organizational culture, human capital, organizational agility, change management, information and communication technology. Also, factors affecting entrepreneurship include human factors, entrepreneurship culture, motivational factors, accelerating variables, information technologies, and rehabilitation variables. Conclusion: It seems that, considering the need for current organizations to benefit from the move towards entrepreneurial management and agility, managers will pay particular attention to the effective dimensions of this management style identified in the present study, providing resources, facilities, and equipment needed to achieve these dimensions can help to continually improving the organization.