Suture Material Research Articles

PENİL KURVATÜR NEDENİYLE NESBİT OPERASYONU YAPILAN HASTALARIN MEMNUNİYET DÜZEYLERİ VE CERRAHİ SONUÇLARI

OBJECTIVE: In this study, it is aimed to evaluate the satisfaction level and surgical results of patients who were performed Nesbit operation due to penile curvature. MATERIAL AND METHODS: Patients who were performed Nesbit corporoplasty on the penile convex surface due to penile curvature were included in a single center between January 2017 and March 2023 in the study. In the preoperative period, the patients' International Erectile Function Index erectile function (IIEF-EF) score, penile curvature angle, penile length and curvature location were evaluated. In the postoperative period, satisfaction level, penile deformity, penile length and IIEF-EF scores were evaluated every three months. RESULTS: 40 patients who developed penile curvature due to 30 Peyronie's disease and 10 congenital penile curvature disease were included in our study. Curvature recurrence was observed in 11 (27.5%) patients in the postoperative period. There were eight (20%) patients whose curvature angle was less than 20 degrees. Postoperative complications were observed in 9 (22.5%) patients and erectile functions were preserved in 97.5% of the patients. While the preoperative IIEF-EF score was 22.08 ± 3.46, it increased to 24.02 ± 3.06 in the postoperative period, and postoperative erectile functions were found to be significantly higher according to the paired sample t test (p = 0.003). While the mean penile length was 13.2 ± 0.57 cm in the preoperative period, it was 11.8 ± 0.66 cm in the postoperative period (p <0.001). The level of patient satisfaction measured using the visual analog scale was found to be 77.37 ± 19.15. The most common complications were penile hypoesthesia and suture sensation. CONCLUSIONS: Nesbit’s procedure is an effective and safe option in the treatment of penile curvature. Penile hypoesthesia and ‘sensation of suture material’ are the two most frequent post-procedural complaints.

A Review on Polymers for Biomedical Applications on Hard and Soft Tissues and Prosthetic Limbs.

In the past decades, there has been a significant increase in the use of polymers for biomedical applications. The global medical polymer market size was valued at USD 19.92 billion in 2022 and is expected to grow at a CAGR of 8.0% from 2023 to 2030 despite some limitations, such as cost (financial limitation), strength compared to metal plates for bone fracture, design optimization and incorporation of reinforcement. Recently, this increase has been more pronounced due to important advances in synthesis and modification techniques for the design of novel biomaterials and their behavior in vitro and in vivo. Also, modern medicine allows the use of less invasive surgeries and faster surgical sutures. Besides their use in the human body, polymer biomedical materials must have desired physical, chemical, biological, biomechanical, and degradation properties. This review summarizes the use of polymers for biomedical applications, mainly focusing on hard and soft tissues, prosthetic limbs, dental applications, and bone fracture repair. The main properties, gaps, and trends are discussed.

Flexor Tendon Injuries and Hand Tendinorrhaphy: A Comprehensive Review

Flexor tendon injuries of the hand represent a challenging clinical entity with significant functional implications. This comprehensive article delves into the intricate realm of flexor tendon injuries, shedding light on the pathophysiology, diagnostic approaches, and treatment modalities, with a primary focus on the surgical technique of tendinorrhaphy. The article commences with an exploration of the anatomy and biomechanics of flexor tendons, elucidating the pivotal role they play in hand function. It subsequently outlines the diverse etiologies that lead to tendon injuries, encompassing traumatic, iatrogenic, and spontaneous causes, while emphasizing the importance of prompt and accurate diagnosis through clinical assessment, imaging, and functional testing. A detailed exposition of the principles and techniques of tendinorrhaphy follows, elucidating the surgical intricacies involved in repairing damaged flexor tendons. The article discusses suture materials, repair methods, and rehabilitation protocols, highlighting the importance of postoperative care and meticulous hand therapy in optimizing outcomes. Furthermore, this article critically reviews emerging trends and innovations in the field, including the use of tissue engineering, biomaterials, and minimally invasive approaches, all of which hold promise for enhancing the management of flexor tendon injuries. In conclusion, this article offers a comprehensive overview of flexor tendon injuries in the hand and underscores the pivotal role of tendinorrhaphy as a cornerstone in their treatment. With a holistic perspective on the subject, it provides clinicians and researchers with a valuable resource to navigate the intricacies of this challenging clinical scenario and fosters ongoing advancements in the field of hand surgery.

The Use of Cholangioscopy In the Diagnosis of Indeterminate Biliary Strictures: A Case Report and Review of the Literature

Biliary strictures or narrowing of a portion of the biliary tree can have benign and malignant etiologies. One third of these will originate from iatrogenic injury and these are typically managed endoscopically. We present the case of a 76-year-old man with history of laparoscopic cholecystectomy ten years prior, who presented with one week of jaundice, dark urine, and pruritus. Along with non-invasive biliary imaging, he underwent cholangioscopy during endoscopic retrograde cholangiopancreatography revealing inflammatory-appearing stenosis of the common hepatic duct with embedded suture material. The stenotic area was biopsied using mini forceps and subsequently dilated with a balloon dilator. A plastic biliary stent was placed with improvement in the patient’s hepatic function, with final cytology and pathology being negative for malignancy. Cholangioscopy facilitated detailed visual inspection of strictures and biopsies under direct visualization. The visual impression increased the sensitivity of the biopsy and assisted in ruling out malignancy from the most critical portion of the stricture. While timing of cholangioscopy remains to be determined, its use is a valuable tool in the diagnosis of indeterminate biliary strictures.

SAT321 Ectopic ACTH Cushing's Syndrome Caused By A Large-cell Neuroendocrine Lung Carcinoma Responding To Desmopressin

Abstract Disclosure: S. Larose: None. D. Rioux: None. R. Albadine: None. A. Lacroix: None. Background: Ectopic ACTH secretion (EAS) is a less frequent cause of ACTH-dependent Cushing’s syndrome (CS), most often caused by a thoracic neuroendocrine tumor (NET). Large-cell neuroendocrine carcinomas (LCNEC) with EAS are rare and usually present a more severe phenotype of ACTH secretion and hypercortisolism. Clinical Case: We report the case of a 44-yo non-smoking man, who presented clinical and biochemical evidence of ACTH-dependent severe CS complicated by pulmonary emboli. In the context of an initial equivocal pituitary MRI, desmopressin and CRH stimulation tests were performed. Desmopressin produced a 157% increase in ACTH (basal: 59.0 pmol/L to peak: 151.9 pmol/L) and a 25% increase in cortisol from baseline; there was no stimulation of ACTH or cortisol during a CRH test and no suppression with high dose dexamethasone tests. Repeat pituitary MRI identified a 5 mm lesion, but inferior petrosal venous sinus sampling (IPSS) under desmopressin did not identify a central ACTH source. Thorax and abdominal imaging including Indium-111 octreoscan identified a left lung 3 mm micronodule which became more evident when pulmonary emboli resolved. In this context of probable ectopic ACTH secretion, the patient was treated with left inferior lung lobectomy for a suspected NET. Pathological studies confirmed a lung LCNEC with morphologic features of carcinoid tumor and strongly positive ACTH immunohistochemistry (IHC) in the primary lesion and lymph node metastasis. The patient was in CS remission after surgery and adjuvant chemotherapy, but developed a CS recurrence 9.5 years later, with a morning ACTH of 56.9 pmol/L, (N <11.0 pmol/L), a non-suppressed AM cortisol of 633 nmol/L after 1mg of dexamethasone overnight and urinary free cortisol of 1717 nmol/24 hours (N:< 789). Imaging showed signs of recurrence, with a hypermetabolic, 14 x 11 mm left hilar lymph node lesion on a TEP-DOTATATE scan in the left lung, near the surgical suture. An endobronchial ultrasound-guided biopsy confirmed the recurrence of the LCNEC. Surgery was performed and demonstrated again positive ACTH IHC of the lesion. The patient achieved remission of CS with secondary adrenal insufficiency, treated with hydrocortisone. Six months later, there was no response of ACTH or cortisol to a desmopressin test. Conclusion: This is the first report of LCNEC with morphologic feature of carcinoid tumor of the lung with ectopic ACTH secretion stimulated by desmopressin. Long delay prior to metastatic recurrence indicates relatively indolent NET. This case report indicates that response to desmopressin can occur in malignant LCNEC. Combining the desmopressin test with other dynamic tests, sellar MRI, IPSS and thoraco-abdominal functional imaging improves diagnostic accuracy of ectopic ACTH source. Presentation: Saturday, June 17, 2023

Clip migration after minimally-invasive surgical procedures

Introduction. Today absorbable suture material as well as plastic nonabsorbable clips are widely used for surgical haemostasis and stabilisation of sutures during minimally invasive operations, however, they are capable of potential and spontaneous migration into the lower and upper urinary tract.Objective. In this paper, we present clinical cases of clip migration after radical prostatectomy and renal resection.Clinical cases. Clinical cases of spontaneous clip migration after the two most common minimally invasive interventions: kidney resection with subsequent removal of a foreign body after the use of lithokinetic therapy and a case of hemostatic material migration after robot-assisted radical prostatectomy. The literature review is also available.Conclusion. Excessive use of non-absorbable plastic clips around their close contact with the kidney cavity or the bladder wall should be avoided to achieve adequate hemostasis during minimally invasive methods of treating patients.

The potential of cell-assembled extracellular matrix for biological sutures: A promising innovation.

Most surgical procedures require using suture materials that are mechanically efficient and accepted by the patient's body. These sutures are essentially composed of synthetic polymers. However, once implanted in patients, they are recognized as foreign bodies and generate chronic inflammation. Thereafter, the patient's immune system will degrade, encapsulate, or even expel the materials. Our innovation, the Cell-Assembled extracellular Matrix (CAM), synthesized from mesenchymal cells, replicates native tissue environments and promotes integration, reducing complications. In a recent study, we introduced CAM-based biological sutures, demonstrating favorable mechanical properties and vascular surgery compatibility. Controlled culture duration tailors CAM for specific applications. Diverse CAM-based suture models were ex vivo tested in animal aorta anastomoses, confirming compatibility. In vivo carotid anastomoses in sheep validated the clinical significance of these innovative sutures. CAM sutures, derived from immunologically favorable allogeneic fibroblast cells, offer high biocompatibility and exhibit superior mechanical properties compared to synthetics by reducing permeability and increasing burst resistance. In vivo testing in sheep underscores clinical applicability, achieving hemostasis and immediate complication prevention. Importantly, CAM-based sutures are compatible with existing vascular surgery techniques, facilitating adoption by surgeons. In conclusion, our findings underscore the effectiveness and clinical significance of these innovative biological sutures.

Conservative Treatment Outcomes for Achilles Tendon Re-Rupture Occurring in the Subacute Phase after Primary Repair

Category: Ankle; Sports Introduction/Purpose: Achilles tendon re-rupture (ATRR) during the subacute postoperative phase is the rupture of the repaired site combined with suture material failure induced by an unexpected traumatic event during postoperative months one to three after primary Achilles tendon repair. This study aimed to determine the effectiveness of conservative treatment for subacute ATRR. Methods: We retrospectively reviewed 390 cases (385 patients) who underwent primary Achilles tendon repair between January 2010 and August 2021. All patients had more than 12 months of follow-up and were divided into two groups based on the development of subacute ATRR (Group 1: 370 cases without ATRR; Group 2: 20 cases with ATRR). Then, we compared several outcome parameters between the groups after administering conservative treatment to those with ATRR. The baseline timepoints in Groups 1 and 2 were the primary repair and re-injury dates. Results: After primary Achilles tendon repair, 5.13% of patients experienced subacute ATRR. The time to performing a single heel raise (SHR) and performing ten repetitive SHRs did not differ between the groups (P = 0.281, 0.486). Likewise, isokinetic dynamometer results showed that the peak torque of 30o/s and 120o/s plantar flexions (the absolute value and the percentage to the contralateral side) also did not differ between the groups (P >0.05, each). However, the early Achilles tendon Total Rupture Score scale and the Foot and Ankle Ability Measure scores before six months were significantly lower in Group 2 than in Group 1 (both P < 0.05); at 12 months, the scores did not differ between the two groups (both P >0.05). Conclusion: Conservative treatment for subacute ATRR after primary Achilles tendon repair has comparable clinical outcomes to those without ATRR.

Absorbable vs Nonabsorbable Sutures for Achilles Tendon Repair: A Systematic Review and Meta-analysis.

Nonabsorbable sutures are still the main choice for acute Achilles tendon rupture (AATR) repair due to strength provided. However, the rerupture rates, infection risks, foreign body reaction, and postsurgical recovery differences between absorbable and nonabsorbable suture materials in AATR repair have not been carefully reviewed. A systematic review was done on PubMed, EBSCO, Cochrane Central Register of Controlled Trials, and Embase to find research studies in relation to complications associated with AATR repair using the PRISMA guidelines. The risk of bias from each study included will be assessed using the Cochrane Risk of Bias Tool for randomized study (RoB 2) and Risk of Bias in Non-Randomized Studies of Interventions (ROBINS-I) for nonrandomized study. Five studies with a total of 255 patients, 105 in the absorbable suture group and 150 in the nonabsorbable suture group, were included for analysis. Risk of rerupture, infection, and foreign body reaction shown no significant difference between groups, and the mean difference of recovery scores were similar. Existing literature shows that absorbable sutures appear to be associated with similar outcomes to nonabsorbable sutures regarding rates of rerupture, infection, foreign body reaction, and outcomes grading following surgical repair of acute Achilles tendon repair.

Role of adipose-derived stem cells in healing surgically induced trauma of the rat's tunica albuginea.

Injection of adipose-derived stem cells (ADSCs) into the injured tunica albuginea (TA) may prevent fibrosis, restore the balance between pro- and antifibrotic pathways, and potentially mitigate erectile dysfunction caused by abnormal TA healing. To assess the potential role of ADSC injection on structural, ultrastructural, functional, and molecular changes in surgically induced trauma of the rat's TA. Forty adult male albino Wistar rats were divided into 5 groups of 8 rats each: group 1, sham; group 2, injury to TA without treatment; group 3, injury to TA and suture repair; group 4, injury to TA and injection of ADSCs without suture repair; group 5, injury to TA followed by injection of ADSCs and suture repair. After 6 weeks, all groups were subjected to functional, histologic, and ultrastructural examination and molecular expression of healing growth factors. The intracavernous pressure (ICP; mean ± SD) was 114 ± 2, 32 ± 2, 65 ± 2, 68 ± 2, and 111 ± 2mm Hg in groups 1 to 5, respectively. There were significant differences in ICP between each of groups 3 to 5 and group 2 (P < .05), and groups 3 and 4 each had significant differences with group 1 (P < .05). No significant difference in ICP occurred between groups 3 and 4 (P > .05). There were significant histologic and ultrastructural alterations in tunical tissues from group 2; however, these changes were markedly less in group 5 in terms of lower levels of fibrotic changes, elastosis, and superior overall neuroendothelial expression. Groups 3 and 4 showed improved structural and ultrastructural parameters when compared with group 2. Group 5 demonstrated lower levels of transforming growth factor β1 and basic fibroblast growth factor expression. This experimental model may encourage administration of ADSCs to prevent the deleterious effects of trauma to the TA. Injecting ADSCs can improve the healing process and erectile dysfunction in a rat model following TA injury, and combining ADSC injection with surgical suturing resulted in superior outcomes. The main limitation was the absence of long-term ICP measurements and a longer follow-up period that may provide further insight into the chronic phase of the healing process. ADSC injection may prevent structural, ultrastructural, functional, and molecular alterations in surgically induced trauma of the rat's TA and enhance the effect of tunical suturing after trauma.

Polypropylene mesh versus nylon mosquito net mesh for external abdominal hernias repair in cattle and buffaloes

Herein, the effectiveness of locally sourced and autoclaved nylon mosquito net mesh for the repair of external abdominal hernias was investigated. Eleven animals (7 cattle and 4 buffaloes) having hernial ring sizes of 8 to 30 cm in diameter were divided into two Groups 1 (5 animals) and Group 2 (6 animals). In Group 1, nylon mosquito net mesh was used, and in Group 2, polypropylene mesh was used for the repair of defects. The animals were sedated with xylazine HCl and given local anaesthesia during surgery. Over the external hernial sac, an elliptical skin incision was made. The internal hernial sac was bluntly dissected and freed down to the hernial ring. The internal hernial sac was opened, and the hernial ring was repaired with nylon mosquito net mesh or polypropylene mesh using the inlay technique. After hernioplasty, the sac and subcutaneous tissue were repaired with prolene suture material. The skin incision was closed with simple interrupted mattress sutures using monofilament polyamide suture material. Antibiotics and analgesics were given postoperatively, and povidone iodine was used to treat the suture line daily. The skin sutures were removed on the 10th to 15th postoperative day, depending upon the wound condition. Mild inflammatory swelling in Group 1 was observed at the incision line, which had completely subsided by the 15th postoperative day. The wounds healed by first intention in both groups. In any case, no recurrence was seen in a follow-up period of 3 months. Without clinical signs of wound dehiscence, infection, or recurrence, all animals recovered uneventfully.

Art of Suturing Techniques

Suturing is necessary and important event in both oral and general surgeries [1]. The term ‘Suture’ describes any strand of material utilized to ligate blood vessels or approximate tissues without the necessity of a mechanical Support [2]. Moreover, Surgical Suture is nothing. but isolating the healing centre, promoting the circulation process, controlling haemostasis stabilising the tissues and the requested position. Various methods &amp; materials used for precise flap placement [3, 4].

THE CURRENT STATE OF THE DEPARTMENT OF GENERAL AND EMERGENCY SURGERY DURING THE PERIOD OF MARTIAL LAW IN UKRAINE

Abstract. Medical work in wartime has its own peculiarities. Among the patients admitted to the clinic for treatment, the number of operations for primary treatment of wounds, necroctomy and amputation of limbs and fingers, removal of foreign bodies (bullets, fragments, glass, etc.), treatment of combined injuries of soft tissues with bone fractures, and others is increasing sharply. The use of VAC and pulse-VAC devices, ultrasonic cavitators, dermatomes, micro-instruments and suture material for microsurgical interventions is very important for the preparation of surgical wounds for reconstructive and restorative operations.

EXPERIMENTAL DEVELOPMENT OF THE METHOD OF SEAMLESS CONNECTION OF THE DURA MATER USING HIGH-FREQUENCY BIPOLAR ELECTRIC WELDING AND SUBSTANTIATION OF ITS GREATER TIGHTNESS, COMPARED TO THE TRADITIONAL METHOD OF CONNECTION USING SUTURE MATERIAL

One of the main tasks of surgical treatment of patients with frontal sinus neoplasms with intracranial spread is to seal the subdural space, which is achieved by hermetically connected dura mater. The purpose of this study was to investigate the structure of the tissues of the dura mater and dura mater-fascia junction using bipolar electric welding. The obtained data shows, that electric welding provided a stable advantage in the tensile strength and tightness of the electric welded connection, compared to the connection using suture material. The value of the tensile strength for the connection of the DM with the DM was established - 32.795±0.1 (increase in strength compared to the suture connection by 1.35 times) mm Hg, for the connection of the DM with the aponeurosis of the skull 30, 71±0.37 (1.26 times increase in strength compared to the suture connection), for the connection of DM with the fascia lata was 29.67±0.33 mmHg. (increase in strength compared to a seam connection by 1.22 times). Also the methods of light microscopy and infrared spectroscopy were used. When evaluating the results, no β-structured aggregates were observed in the tissue samples under study. There was minimal traumatic damage to the adjacent dura mater tissue and minimal thermal damage from vaporisation. Pacchioni granulations, venous pial, and cortical vessels of the dura mater were preserved. The study observed complete identity of the wave values of amide A, B, amide I, II spectra; preservation of amide A, B peaks. Preservation and curvature of the N-H arm and O=C-N band were changed within acceptable limits. The findings indicate the absence of β-structured protein aggregates in the junction area, which excludes the possibility of fibrous structures and, as a result, does not contribute to the formation of a meningeal scar. The structure of the collagen protein in the junction area is normal, altered but intact, with preservation of its functions. This indicates the feasibility of using the method of high-frequency bipolar electric welding for surgical closure of defects of the dura mater, as an alternative to closure with suture material

Addition of Transfixation Suture to Purse String Suture During Intraperitoneal Inguinal Hernia Repair Increases Peri-Hernia Sac Neck Collagen Formation

BackgroundThe worldwide accepted repair for indirect inguinal hernia in children is high ligation of the hernia sac with open herniotomy. However, laparoscopic pediatric inguinal hernia repair (IHR) has been gaining popularity in the last two decades. An experimental study was conducted to investigate the effects of different intraperitoneal IHR suture techniques on the collagen formation at the hernia sac neck. MethodsPresent study was conducted on thirty-five male adult (3–6 months old) Wistar-Albino rats (260–300 g). Intraperitoneal IHR with different hernia sac neck suturing techniques (purse string suture only, transfixation suture only and purse string suture plus transfixation suture) were performed through median laparotomy using open operative techniques. Non-absorbable 2/0 braided polyester suture with 16 mm 1/2 curved round needle (Ti-cron, Covidien, MN) was used as suture material. ResultsThe highest collagen thickness around the suture was detected in intraperitoneal IHR with purse-string plus transfixation suture group. The collagen thickness of the intraperitoneal IHR with purse string suture only and IHR with tranfixation suture only groups were not statistically significantly different. The collagen thickness of the intraperitoneal IHR with purse string suture plus transfixation suture group was statistically significantly higher compared with the intraperitoneal IHR with purse string suture only and intraperitoneal IHR with transfixation suture only groups. ConclusionsThe combined usage of purse string suture and transfixation suture during laparoscopic intraperitoneal inguinal hernia repair further stimulates mesothelial fibrosis at the hernia sac neck compared with mesothelial fibrosis induced by purse string suture only or transfixation suture only.

In Vivo Time-Lapse Observation of PLA/CL and PVDF Surgical Sutures by Optical Coherence Tomography

In Vivo Time-Lapse Observation of PLA/CL and PVDF Surgical Sutures by Optical Coherence Tomography

General Morphological Characteristics of the Results of Experimental Modeling of Aseptic Peritonitis

BackgroundAseptic peritonitis is a reaction of the local immune system aimed at rejection of a foreign body, which, having antigenic properties, does not (unlike a pathogen) counteract the immune system. The suture materials, namely catgut thread, used in intracavitary surgical operations possess xenogenic properties and can be used for antigenic stimulation of the immune system of the peritoneal cavity. Consequently, we decided to use a catgut suture for antigenic stimulation of the immune system of the peritoneal cavity and to study the morphological features of the results of experimental modeling of aseptic peritonitis in albino rats. MethodThe study involved 15 Wistar albino male rats, weighing 286,13 ± 6,26 g. To study the dynamics of destructive changes made by the catgut implant in the peritoneal cavity of the experimental animals, the animals were assigned into three groups in accordance with the time interval of their euthanasia at 3, 7, and 14 days of the experiment. ResultsAfter modeling an aseptic peritonitis, the investigation the abdominal cavity showed that in four out of five animals of the first group, that is, on day 3 of the experiment, the catgut implant had adhered to the greater omentum. The search for the fifth implant led to an unexpected discovery: we found it conjoined with the second derivative of the visceral peritoneum, similar in structure to the greater omentum, but related to the testes. On day 7, the implant embedded into the peritoneal cavity of the animals, had adhered to the serous formations of the testes in all five cases (100%, three of them – to the left epididymal omentum, and another two – to the right one). On day 14 (n = 5) it was found that in three cases it had adhered to the serous formations of the testes (60%, one of them to the left epididymal omentum, another two – to the right one) and in two cases it had adhered to the greater omentum (40%). ConclusionDuring the experiment on implantation of a xenogenic substrate in the form of flat bundles made from the catgut thread into the peritoneal cavity of sexually mature male rats, it was found for the first time that their acceptors were not only the greater omentum, but also two derivatives of the peritoneum, homeomorphic to it and associated with the epididymides, which we reasonably called epididymal omenta and described in details.

Pressure-Spun Fibrous Surgical Sutures for Localized Antibacterial Delivery: Development, Characterization, and In Vitro Evaluation.

Surgical sutures designed to prevent infection are critical in addressing antibiotic-resistant pathogens that cause surgical site infections. Instead of antibiotics, alternative materials such as biocides have been assessed for coating commercially used sutures due to emerging antibiotic resistance concerns worldwide. This study has a new approach to the development of fibrous surgical sutures with the ability to deliver localized antibacterial agents. A new manufacturing process based on pressure spinning was used for the first time in the production of fibrous surgical sutures by physically blending antibacterial triclosan (Tri) agent with poly(lactic-co-glycolic acid) (PLGA) and poly(ethylene oxide) (PEO) polymers. Fibrous surgical sutures with virgin PLGA, virgin PEO, different ratios of PLGA-PEO, and different ratios of Tri-loaded PLGA-PEO fibrous sutures were produced to mimic the FDA- and NICE-approved PLGA-based sutures available in the market and compared for their characteristics. They were also tested simultaneously with commercially available sutures to compare their in vitro biodegradation, antibacterial, drug release, and cytotoxicity properties. After in vitro antibacterial testing for 24 h, sutures having 285 ± 12 μg/mg Tri loading were selected as a model for further testing as they exhibited antibacterial activity against all tested bacteria strains. The selected model of antibacterial fibrous sutures exhibited an initial burst of Tri release within 24 h, followed by a sustained release for the remaining time until the sutures completely degraded within 21 days. The cell viability assay showed that these surgical sutures had no cytotoxic effect on mammalian cells.

The type of suture material affects transverse aortic constriction-induced heart failure development in mice: a repeated measures correlation analysis.

Transverse-aortic constriction (TAC) operation is a widely used animal model to induce hypertrophy and heart failure through left-ventricular pressure overload. In mice, the cardiac response to TAC exhibits considerable variability influenced by factors such as strain, sub-strain, age, sex and vendor. To investigate the impact of suture material (silk versus prolene) and size (6-0 versus 7-0) on the TAC-induced phenotype, we performed surgeries on male C57BL6/N mice at 9 weeks of age defining the aortic constriction by a 27G needle, thereby employing most frequently used methodological settings. The mice were randomly assigned into four separate groups, 6-0 silk, 7-0 silk, 6-0 prolene and 7-0 prolene (10 mice per group). Echocardiography was conducted before TAC and every 4 weeks thereafter to monitor the development of heart failure. Repeated measures correlation analysis was employed to compare disease progression among the different groups. Our findings reveal a significant influence of the chosen suture material on TAC outcomes. Mice operated with prolene showed increased mortality, slower body weight gain, faster left-ventricular mass increase, and a faster decline in left-ventricular ejection fraction, fractional shortening and aortic pressure gradient compared to silk-operated mice. Moreover, despite non significant, using thinner suture threads (7-0) tended to result in a more severe phenotype compared to thicker threads (6-0) across all tested parameters. Collectively, our results highlight the importance of suture material selection in determining the cardiac phenotype induced by TAC and emphasize the need to consider this factor when comparing data across different research laboratories.

An Experimental Design to Compare the Compatibility of Absorbable and Nonabsorbable Suture Materials in the Lateral Canthus Area.

Polyglactin 910 (Vicryl) and polypropylene (Prolene) are common suture materials used in ophthalmic surgical procedures. However, there are limited studies assessing tissue reactions to different sutures in eyelid areas. In this study, these suture materials were used subcutaneously in 10 lateral canthi of 5 pigs. Clinical reactive score (CRS) was assessed 14 days and 60 days after surgery. On histology, subcutaneous tissue reactions were assessed by the presence of different inflammatory cells, and the aggregate tissue irritation score was calculated. CRS and the histology score did not differ between these sutures. None of the suture materials used were superior for use in the lateral canthi of pig eyes. Therefore, we recommend both of these sutures for use in the subcutaneous tissue of the eyelid area in animal experiments or clinical practice.