Membrane Surface Tension Research Articles

Simple differences in the protein-membrane attachment mechanism have functional consequences for surface mechanics.

This paper describes two methods for propagating coupled membrane and embedded particle dynamics with ensembles that are valid to second order in the deformation of the membrane. Proteins and functional lipids associate with cellular membranes, and their attachments influence membrane physical and dynamical properties. Therefore, it is necessary to accurately model the coupled dynamics of the membrane and any associated material of interest. We have developed two methods for coupling membrane and particle dynamics that differ in the binding mechanism of the particle to the surface. The "on-surface" mechanism should be used for particles that slide along the membrane; this description leads to an effective reduction in the membrane surface tension. The "in-surface" mechanism treats the particles as tightly bound to the lipidic binding sites; the method avoids double counting lateral entropy of implicitly modeled lipids. We emphasize the differences between these two mechanisms, when it is appropriate to use them, and how the methods differ from previously used dynamic methods.

A Tense Situation: Maintaining ER Homeostasis during Lipid Droplet Budding

In this issue of Developmental Cell, Chorlay etal. (2019) provide evidence that asymmetric membrane surface tension determines the directionality of lipid droplet (LD) emergence. Furthermore, phospholipid synthesis "refills" the outer leaflet of the endoplasmic reticulum (ER) membrane to maintain cytosolic LD emergence and prevent disruptions to ER homeostasis.

Design of Small Nanoparticles Decorated with Amphiphilic Ligands: Self-Preservation Effect and Translocation into a Plasma Membrane.

Design of nanoparticles (NPs) for biomedical applications requires a thorough understanding of cascades of nano-bio interactions at different interfaces. Here, we take into account the cascading effect of NP functionalization on interactions with target cell membranes by determining coatings of biomolecules in biological media. Cell culture experiments show that NPs with more hydrophobic surfaces are heavily ingested by cells in both the A549 and HEK293 cell lines. However, before reaching the target cell, both the identity and amount of recruited biomolecules can be influenced by the pristine NPs' hydrophobicity. Dissipative particle dynamics (DPD) simulations show that hydrophobic NPs acquire coatings of more biomolecules, which may conceal the properties of the as-engineered NPs and impact the targeting specificity. Based on these results, we propose an amphiphilic ligand coating on NPs. DPD simulations reveal the design principle, following which the amphiphilic ligands first curl in solvent to reduce the surface hydrophobicity, thus suppressing the assemblage of biomolecules. Upon attaching to the membrane, the curled ligands extend and rearrange to gain contacts with lipid tails, thus dragging NPs into the membrane for translocation. Three NP-membrane interaction states are identified that are found to depend on the NP size and membrane surface tension. These results can provide useful guidelines to fabricate ligand-coated NPs for practical use in targeted drug delivery, and motivate further studies of nano-bio-interactions with more consideration of cascading effects.

Surface tension of membranes depending on the boundary shape

In this paper, we study the boundary effect on the surface (or frame) tension of elastic membrane surface models. The frame tension generally depends only on the projected area of the boundary over which the surface spans. However, from a spin model analogy, the frame tension is expected to be dependent also on the boundary shape at the continuous transition point. We confirm this expectation using the following fixed-connectivity and tethered surface models: the surface model of Helfrich and Polyakov and a surface model with deficit angle term. We also discuss the reason why this expectation is worthwhile to study.

Directional and Rotational Motions of Nanoparticles on Plasma Membranes as Local Probes of Surface Tension Propagation.

Mechanical heterogeneity is ubiquitous in plasma membranes and of essential importance to cellular functioning. As a feedback of mechanical stimuli, local surface tension can be readily changed and immediately propagated through the membrane, influencing structures and dynamics of both inclusions and membrane-associated proteins. Using the nonequilibrium coarse-grained membrane simulation, here we investigate the inter-related processes of tension propagation, lipid diffusion, and transport of nanoparticles (NPs) adhering on the membrane of constant tension gradient, mimicking that of migrating cells or cells under prolonged stimulation. Our results demonstrate that the lipid bilayer membrane can by itself propagate surface tension in defined rates and pathways to reach a dynamic equilibrium state where surface tension is linearly distributed along the gradient maintained by the directional flow-like motion of lipids. Such lipid flow exerts shearing forces to transport adhesive NPs toward the region of a larger surface tension. Under certain conditions, the shearing force can generate nonzero torques driving the rotational motion of NPs, with the direction of the NP rotation determined by the NP-membrane interaction state as functions of both NP property and local membrane surface tension. Such features endow NPs with promising applications ranging from biosensing to targeted drug delivery.

Extracting lipid vesicles from plasma membranes via self-assembly of clathrin-inspired scaffolding nanoparticles.

Single-cell analysis is a new and rapidly expanding field, the goal of which is obtaining fresh information from individual cells to understand the regulatory mechanisms of cell development and diseases. Conventional approaches generally rely on the cell lysis which, however, is destructive to cells and against multiple sampling from the living cell. Here, we propose and design a scaffolding nanoparticle (NP) system that enables us to sample cytoplasmic contents without rupturing the cellular membrane, by mimicking the unusual features of clathrin. Our simulation results reveal the design principles, following which scaffolding NPs can extract lipid vesicles from plasma membranes, with both the pathway and the mechanism resembling the clathrin-mediated endocytosis, i.e. multiple NPs deposit at the membrane, assembling into cage-like structures to deform the membrane into a vesicle shape. As important design parameters, the interaction between different NPs should be properly stronger than that between each NP and the membrane to ensure the cage formation, and optimal NP concentration and the membrane surface tension are also requisite for extracting lipid vesicles. Our results provide useful guidelines for design of bio-inspired scaffolding NPs as an intelligent machine for practical use in but not limited to the single-cell analysis.

Fixed Boundary Vibration Measurement Method for Surface Tension of Membrane Structure

At present, some methods for measuring the surface tension of membrane structure are applied to engineering practice. But there are some defects happened in the engineering practice. This paper proves the partial boundary of the fixed membrane surface. The natural vibration frequency of the membrane surface is only related to the membrane surface tension. Based on this principle, a magnetic clip-type membrane surface fixed boundary fixture is designed. A method for measuring the tension of the membrane surface tension by measuring the natural vibration frequency of the inner membrane surface of the fixed boundary is proposed. The finite element method was used to analyze the vibration frequency of the fixed boundary membrane under different tensions. The vibration frequency of the fixed boundary membrane under different tensions was measured by experiments. The simulation results were compared with the experimental results, and the accuracy of the method was verified. Through this method, the calibration result of the small sample on the calibration table can be used for the tension measurement of any boundary condition in the actual project and the material film structure, which is in accordance with the requirements of simple operation, wide applicability and high measurement precision in engineering practice.

Experimental Study and Simulation of Film Tension Measurement by Apex Method

In order to ensure the normal use of the tensioned membrane structure, it is necessary to effectively detect the tension applied to the tensioned membrane surface. In this paper, a top-projection membrane surface tension measuring instrument with fixed boundary was designed. During the measure process, the force and the apex displacement were measured simultaneously to obtain the calibration curve group of force and abutment displacement. Compared with the traditional apex method, the measuring instrument eliminates the influence of the membrane structure boundary on the measurement results, and the measurement results were accurate and reliable. The correctness of the device is verified by numerical simulation and experimental results. Theoretically solved the technical problem of rapid measurement of membrane surface tension, and obtained stable results in actual measurement, thus providing a new method for membrane surface tension measurement.

Frontiers of Complex Disease Mechanisms: Membrane Surface Tension May Link Genotype to Phenotype in Glaucoma.

Although many monogenic diseases are understood based upon structural changes of gene products, less progress has been made concerning polygenic disease mechanisms. This article presents a new interdisciplinary approach to understand complex diseases, especially their genetic polymorphisms. I focus upon primary open angle glaucoma (POAG). Although elevated intraocular pressure (IOP) and oxidative stress are glaucoma hallmarks, the linkages between these factors and cell death are obscure. Reactive oxygen species (ROS) promote the formation of oxidatively truncated phosphoglycerides (OTP), free fatty acids, lysophosphoglycerides, oxysterols, and other chemical species that promote membrane disruption and decrease membrane surface tension. Several POAG-linked gene polymorphisms identify proteins that manage damaged lipids and/or influence membrane surface tension. POAG-related genes expected to participate in these processes include: ELOVL5, ABCA1, APOE4, GST, CYP46A1, MYOC, and CAV. POAG-related gene products are expected to influence membrane surface tension, strength, and repair. I propose that heightened IOP overcomes retinal ganglion cell (RGC) membrane compressive strength, weakened by damaged lipid accumulation, to form pores. The ensuing structural failure promotes apoptosis and blindness. The linkage between glaucoma genotype and phenotype is mediated by physical events. Force balancing between the IOP and compressive strength regulates pore nucleation; force balancing between pore line tension and membrane surface tension regulates pore growth. Similar events may contribute to traumatic brain injury, Alzheimer's disease, and macular degeneration.

Role of Surface Tension in Gas Nanobubble Stability Under Ultrasound.

Shell-stabilized gas nanobubbles have recently captured the interest of the research community for their potential application as ultrasound contrast agents for molecular imaging and therapy of cancer. However, the very existence of submicron gas bubbles (especially uncoated bubbles) has been a subject of controversy in part due to their predicted Laplace overpressure reaching several atmospheres, making them supposedly thermodynamically unstable. In addition, the backscatter resulting from ultrasound interactions with nanoparticles is not predicted to be readily detectable at clinically relevant frequencies. Despite this, a number of recent reports have successfully investigated the presence and applications of nanobubbles for ultrasound imaging. The mechanism behind these observations remains unclear but is thought to be, in part, influenced heavily by the biophysical properties of the bubble-stabilizing shell. In this study, we investigated the effects of incorporating the triblock copolymer surfactant, Pluronic, into the lipid monolayer of nanobubbles. The impact of shell composition on membrane equilibrium surface tension was investigated using optical tensiometry, using both pendant drop and rising drop principles. However, these techniques proved to be insufficient in explaining the observed behavior and stability of nanobubbles under ultrasound. Additionally, we sought to investigate changes in membrane surface tension (surface pressure) at different degrees of compression (analogous to the bubble oscillations in the ultrasound field) via Langmuir-Blodgett experiments. Results from this study show a significant decrease ( p < 0.0001) in the nanobubble equilibrium surface tension through the incorporation of Pluronic L10, especially at a ratio of 0.2, where this value decreased by 28%. However, this reduction in surface pressure was seen only for specific compositions and varied with monolayer structure (crystalline phase or liquid-crystalline packing). These results indicate a potential for optimization wherein surface pressure can be maximized for both contraction and expansion phases with the proper lipid to Pluronic balance and microstructure.

Benchmarking of Sterilizing-Grade Filter Membranes with Liposome Filtration.

Cytotoxic drugs can be encapsulated in liposomes vesicles, which act as drug delivery vehicles and reduce the risk of exposure of drug to healthy cells. The sterility of such liposome solutions is typically ensured using 0.2 μm-rated sterilizing-grade membranes, but due to the high viscosity and low surface tension of these formulations, they can cause premature blocking and increased risk of bacterial penetration through a 0.2 μm sterilizing-grade membrane. The low surface tension of liposome solutions affects the contact angle with membrane and reduces bubble point, leading to bacterial penetration through the membrane. This poses a great challenge to select an appropriate sterilizing-grade membrane for a given process and for filter manufacturers to develop a sterilizing-grade membrane that specifically addresses these needs. In this study, the influence of different variables that could affect the total throughput and bacterial retention performance of different membrane types on processing of liposome solutions was evaluated. Based on the results, we conclude that the membrane properties, for example, surface porosity, surface tension, pore size, symmetry/asymmetry, hydrophilicity and liposome properties (e.g., composition, lipid size, and concentration) affect bacterial retention and total throughput capacity. Process parameters such as temperature, pressure, and flow should also be optimized to improve process efficiency.LAY ABSTRACT: Cytotoxic drugs can be encapsulated in liposomes vesicles, which act as drug delivery vehicles and reduce the risk of exposure of drug to healthy cells. Liposome solution cause premature blocking and increased risk of bacterial penetration through a 0.2 μm sterilizing-grade membrane due to their high viscosity and low surface tension. In this study, we demonstrated the total throughput and bacterial retention performance of different sterilizing-grade membranes with liposome solution. Based on the results, we conclude that some sterilizing-grade membranes yield less throughput and bacterial retention compared to other membranes. This is due to liposome formulation and membrane properties. Therefore, it is important to identify the product formulation and membrane properties before selection of a suitable sterilizing-grade filter for a given process application to ensure expected throughput and bacterial retention.

Cell membrane biophysics with optical tweezers.

Membrane elastic properties play important roles in regulating cell shape, motility, division and differentiation. Here I review optical tweezer (OT) investigations of membrane surface tension and bending modulus, emphasizing didactic aspects and insights provided for cell biology. OT measurements employ membrane-attached microspheres to extract long cylindrical nanotubes named tethers. The Helfrich-Canham theory yields elastic parameters in terms of tether radius and equilibrium extraction force. It assumes initial point-like microsphere attachment and no cytoskeleton content within tethers. Experimental force-displacement curves reveal violations of those assumptions, and I discuss proposed explanations of such discrepancies, as well as recommended OT protocols. Measurements of elastic parameters for predominant cell types in the central nervous system yield correlations between their values and cell function. Micro-rheology OT experiments extend these correlations to viscoelastic parameters. The results agree with a quasi-universal phenomenological scaling law and are interpreted in terms of the soft glass rheology model. Spontaneously-generated cell nanotube protrusions are also briefly reviewed, emphasizing common features with tethers. Filopodia as well as tunneling nanotubes (TNT), which connect distant cells and allow transfers between their cytoplasms, are discussed, including OT tether pulling from TNTs which mediate communication among bacteria, even of different species. Pathogens, including bacteria, viruses and prions, opportunistically exploit TNTs for cell-to-cell transmission of infection, indicating that TNTs have an ancient evolutionary origin.

Micromechanical and surface adhesive properties of single saccharomyces cerevisiae cells

The adhesion and mechanical properties of a biological cell (e.g. cell membrane elasticity and adhesiveness) are often strong indicators for the state of its health. Many existing techniques for determining mechanical properties of cells require direct physical contact with a single cell or a group of cells. Physical contact with the cell can trigger complex mechanotransduction mechanisms, leading to cellular responses, and consequently interfering with measurement accuracy. In the current work, based on ultrasonic excitation and interferometric (optical) motion detection, a non-contact method for characterizing the adhesion and mechanical properties of single cells is presented. It is experimentally demonstrated that the rocking (rigid body) motion and internal vibrational resonance frequencies of a single saccharomyces cerevisiae (SC) (baker’s yeast) cell can be acquired with the current approach, and the Young’s modulus and surface tension of the cell membrane as well as surface adhesion energy can be extracted from the values of these acquired resonance frequencies. The detected resonance frequency ranges for single SC cells include a rocking (rigid body) frequency of 330 ± 70 kHz and two breathing resonance frequencies of 1.53 ± 0.12 and 2.02 ± 0.31 MHz. Based on these values, the average work-of-adhesion of SC cells on a silicon substrate in aqueous medium is extracted, for the first time, as . Similarly, the surface tension and the Young’s modulus of the SC cell wall are predicted as and 9.20 ± 2.80 MPa, respectively. These results are compared to those reported in the literature by utilizing various methods, and good agreements are found. The current approach eliminates the measurement inaccuracies associated with the physical contact. Exciting and detecting cell dynamics at micro-second time-scales is significantly faster than the currently known metabolistic response times of cells (milliseconds to seconds), thus, it has the potential to decouple metabolistic and mechanotransduction effects from external stimuli and to operate at high throughput rates.

Study on Surface Tension of Membrane Made in Cellulose Diacetate

The value of surface tension is important for choosing oil in CA fibers production. So indirect method were used to analyze the surface tension of CA fibers in this paper. First, Chips of CA were dissolved in acetone solution with 5%, 10%, 15% three kinds of concentration. The three kinds of concentration of the solution were heated 5 hours with 55°C and then standing and defoaming for 24h. Squeeze the solution with two flat glass slides to make the membranes. Second, choose deionized water, Glycerin, Formamide, Bromonaphthalene and Diiodomethane with different polarity as tested liquids to measure the contact angle of CA membrane with JC2000C contact angle meter. Then, Zisman’s linear extrapolation method was used to measure the critical surface tension of CA membranes. On the other hand, S. Wu’S harmonic equation on average method was used too for comparison. The value of two different methods was the same, 42 mN/m, and the result indicated that the estimated method was reliable.

Salt backwashing of organic-fouled ultrafiltration membranes: Effects of feed water properties and hydrodynamic conditions

In the application of ultrafiltration (UF) membranes, organics can result in severe hydraulically irreversible fouling which is not eliminated effectively by periodic backwashing. Using NaCl solution as backwash water, the effects of feed water properties and hydrodynamic conditions on irreversible fouling caused by humic acid and sodium alginate were systematically investigated. Results showed that NaCl solution backwashing resulted in high hydraulic cleaning efficiencies (HCEs) which were hardly affected by feed concentrations, ionic strengths, solution temperatures, filtration fluxes, backwash fluxes, and backwash intervals (p > 0.05). An alleviated hydraulically irreversible fouling was observed at a low feed concentration, a low ionic strength, a high temperature, a small filtration flux, a large backwash flux, a long backwash duration, and a short backwash interval. Using a constant backwash volume, all the multiple backwash parameters of NaCl solution backwashing led to high and comparable HCEs (∼97%), and the HIFIs were equal statistically (p > 0.05) for a fixed backwash duration or interval. Membrane charge and surface tension analysis suggested that NaCl solution backwashing outperformed UF permeate backwashing by restoring almost characteristics of UF membranes. Scanning electron microscopy observation further revealed the superiority of NaCl solution backwashing to UF permeate backwashing for alleviating organic fouling of UF membranes.

The utilization of round window membrane surface tension in facilitating slim electrodes insertion during cochlear implantation.

This is a prospective randomized study aimed to evaluate the round window membrane (RWM) surface tension in facilitating slim electrodes insertion during cochlear implantation. A total number of (118) children were included in this study (118 implantations). Mean age was 36.72months (range from 18 to 60months). This study was conducted from January 2015 to September 2016 at a cochlear implant centre in a tertiary referral hospital. Slit incision in the anterosuperior quadrant of the RWM was done in 70 cases, While RWM cruciate incision was done in 48 cases. Of the 48 patients who underwent RWM cruciate incision, 13 cases had no problem, while in 35 cases, we faced difficult insertion. When slit incision of the RWM was done (70 cases), 68 cases showed smooth insertion, meanwhile, we faced increased operative time due to flopping of the electrode in 2 cases only. Moreover, residual low-frequency hearing preservation was more achieved when slit incision of the RWM was done. Tensile strength of the round window membrane after slit incision of the RWM offers support to slim electrodes during introduction, decreasing incidence of kinking and floppiness, hence shortening the maneuver time and minimizing the number of trials. This facilitates easy smooth slim electrodes introduction, decreasing intracochlear trauma. Moreover, slit incision of the RWM may offer better residual hearing preservations than cruciate incision of the RWM during slim electrodes introduction.

ER Membrane Phospholipids and Surface Tension Control Cellular Lipid Droplet Formation

Cells convert excess energy into neutral lipids thatare made in the endoplasmic reticulum (ER) bilayer. The lipids are then packaged into spherical or budded lipid droplets (LDs) covered by a phospholipid monolayer containing proteins. LDs play a key role in cellular energy metabolism and homeostasis. A key unanswered question in the life of LDsis how they bud off from the ER. Here, we tackle this question by studying the budding of artificial LDsfrom model membranes. We find that the bilayerphospholipid composition and surface tension are key parameters of LD budding. Phospholipids have differential LD budding aptitudes, and those inducing budding decrease the bilayer tension.We observe that decreasing tension favors the egress of neutral lipids from the bilayer and LD budding. In cells, budding conditions favor the formation of small LDs. Our discovery reveals the importance of altering ER physical chemistry for controlled cellular LD formation.

Modeling the Mechanics of Cell Division: Influence of Spontaneous Membrane Curvature, Surface Tension, and Osmotic Pressure.

Many cell division processes have been conserved throughout evolution and are being revealed by studies on model organisms such as bacteria, yeasts, and protozoa. Cellular membrane constriction is one of these processes, observed almost universally during cell division. It happens similarly in all organisms through a mechanical pathway synchronized with the sequence of cytokinetic events in the cell interior. Arguably, such a mechanical process is mastered by the coordinated action of a constriction machinery fueled by biochemical energy in conjunction with the passive mechanics of the cellular membrane. Independently of the details of the constriction engine, the membrane component responds against deformation by minimizing the elastic energy at every constriction state following a pathway still unknown. In this paper, we address a theoretical study of the mechanics of membrane constriction in a simplified model that describes a homogeneous membrane vesicle in the regime where mechanical work due to osmotic pressure, surface tension, and bending energy are comparable. We develop a general method to find approximate analytical expressions for the main descriptors of a symmetrically constricted vesicle. Analytical solutions are obtained by combining a perturbative expansion for small deformations with a variational approach that was previously demonstrated valid at the reference state of an initially spherical vesicle at isotonic conditions. The analytic approximate results are compared with the exact solution obtained from numerical computations, getting a good agreement for all the computed quantities (energy, area, volume, constriction force). We analyze the effects of the spontaneous curvature, the surface tension and the osmotic pressure in these quantities, focusing especially on the constriction force. The more favorable conditions for vesicle constriction are determined, obtaining that smaller constriction forces are required for positive spontaneous curvatures, low or negative membrane tension and hypertonic media. Conditions for spontaneous constriction at a given constriction force are also determined. The implications of these results for biological cell division are discussed. This work contributes to a better quantitative understanding of the mechanical pathway of cellular division, and could assist the design of artificial divisomes in vesicle-based self-actuated microsystems obtained from synthetic biology approaches.

A 3D Multi-Phase Hydrodynamic Model for Cytokinesis of Eukaryotic Cells

AbstractIn the late stage of the mitotic cycle of eukaryotic cells, cytokinesis ensues during which a parent cell replicates its nucleus with the necessary genetical substances (i.e., DNAs and chromosomes) and splits into two similar offspring cells. This mitotic process involves complex chemical, biophysical andmechanical processes whose details are just beginning to be unfolded experimentally. In this paper, we propose a full 3-D hydrodynamical model using a phase field approach to study the cellular morphological change during cytokinesis. In this model, the force along the contracting ring induced by remodeling of actin-myosin filament on cell cortex layer at the division plane of the parent cell during cytokinesis, is approximated using a proxy force anchored on the newly formed nuclei. The symmetric or asymmetric cell division is simulated numerically with the model. Our numerical results show that the location of the division plane and the contracting force along the cytokinetic ring on the division plane are essential for the cell division. In addition, our numerical study also shows that, during cytokinesis, surface tension of the cell membrane also contributes to this process by retaining the morphological integrity of the offspring cells. This model and the accompanying numerical simulation tool provide a solid framework to build upon with more sophisticated whole cell models to probe the cell mitotic process.

Dependence of the surface tension on the shape of surface boundary

We numerically check that the surface tension of membranes is independent of the shape of surface boundary. The surface tension is calculated by means of the Monte Carlo simulation technique on two types of cylinders made of rubans of size L1 and L2, where the rubans are the same for the projected area and different in the ratio L1/L2. The difference of the surface tension disappears in the thermodynamic limit in both models of Helfrich–Polyakov and Landau–Ginzburg.