ABSTRACT Land surface temperature (LST) is an important physical parameter that reflects changes in surface processes. Remote sensing makes it possible to obtain global or regional LSTs with high spatial and temporal resolution. Feng-Yun 3E (FY-3E) is the world’s first civilian dawn-dusk orbiting meteorological satellite on board the Medium Resolution Spectral Imager – Low-Light (MERSI-LL) sensor capable of acquiring LST at 250 m spatial resolution at dawn and dusk. By complementing with existing polar-orbiting satellites, FY-3E can fill the satellite observation gap within the 6-hour assimilation window and further enhance the observation capability of LST. To retrieve accurate LST from MERSI-LL, the generalized split-window (GSW) method was developed. The GSW coefficients were derived from the simulation dataset produced by Moderate Resolution Transmittance Code 5.2 with an atmospheric profile database. As a key input to the GSW algorithm, the land surface emissivity was dynamically estimated by the Advanced Spaceborne Thermal Emission and Reflection Radiometer Global Emissivity Dataset in combination with the fractional vegetation cover. The water vapour content is calculated in real time by the split-window covariance/variance ratio method. The comparison between retrieved LSTs and in situ LSTs shows that the GSW algorithm can effectively retrieve the LSTs of vegetation cover surfaces. The root mean square error ranged from 0.79 to 1.79 K at dawn and from 1.32 to 1.92 K at dusk.