Although surface finish can be a critical variable in clinical performance, there is a dearth of information regarding surface characteristics of teeth prepared for artificial crowns. This study characterized teeth prepared for complete cast restorations using three representative types of rotary instruments. One hundred and five standardized tooth preparations for complete crowns were performed using a modified milling machine on extracted human teech with diamond, tungsten carbide, and tungsten carbide finishing burs of similar shape (n = 35). The prepared dentin was analyzed with a surface profilometer and a scanning electron microscope (SEM). Differences between rotary instrument groups were determined with parametric ANOVA and Tukey's Studentized Range (HSD). Statistically significant differences in the surface topography of prepared teeth were seen. Mean surface roughnesses (Ra) were 8.6 and 6.8 μm for teeth prepared with diamond and tungsten carbide burs. Teeth completed with finishing burs appeared to result in a smoother surface (1.2 μm).