This study aims to explore the potential of olive leaves nanoparticles (OLNPs) in scavenging free radicals, particularly 2,2-diphenyl-1- picryl-hydrazyl-hydrate (DPPH), and their capability to mitigate the effects of ionizing radiation. The in vitro assessment of antioxidant activity revealed promising results for OLNPs, exhibiting effective DPPH scavenging within a concentration range of 0.00002 to 0.0001 g/l. Additionally, the natural nanoparticles demonstrated significant antioxidant capacity, suggesting their potential as radical scavengers against free radical-induced damages. Characterization of OLNPs was conducted using UV-Visible absorption spectroscopy, revealing surface Plasmon resonance absorption peaks around 287 nm. Furthermore, atomic force microscopy was employed to determine the morphology and size of the OLNPs, indicating an average diameter of 65 nm.