Chip Surface Research Articles

Strengthening mechanisms and wear performance of Al/APC/micro-B4Cp hybrid composites prepared by HTCDC process and hot extrusion

An Al/APC/micro-B4Cp hybrid composites were fabricated using the hydrothermal carbonized deposition on chips (HTCDC) process and hot extrusion process. The microstructure and mechanical properties and wear resistance of the Al/APC/micro-B4Cp hybrid composites were characterized. APC spheres and irregular micro-B4Cp could be observed on the Al chips surface after the HTCDC process, and the micro-B4Cp were also coated with an APC film. Al/APC/micro-B4Cp hybrid composites were prepared by hot extrusion from the Al chips covered with APC and micro-B4Cp. With the increase of micro-B4Cp content, the α-Al grain sizes of composites decrease from 18.8 µm to 12.8 µm, because the dispersed micro-B4Cp in the composites can pin the grain boundaries, hinder the migration of grain boundaries, and inhibit the growth of recrystallized grains. The highest values were obtained when the micro-B4Cp content was 3 wt%, and the UTS and hardness values of Al/APC/B4Cp hybrid composites (223.66 MPa and 101.8HV) were increased by 14.07% and 24%, respectively, compared with Al/APC composite. The results of the YS calculation show that thermal mismatch strengthening is the main strengthening mechanism, followed by grain-refined strengthening, and load transfer strengthening. Because the APC of the self-lubricating effect can form a lubrication film on the wear surface during sliding, the COF and wear rate of the Al/APC composite are low. When the micro-B4Cp is added to the matrix, the micro-B4Cp will strip the APC film from the wear surface, so the COF increases. However, the addition of micro-B4Cp can greatly increase the hardness of the composite, as a consequence that the wear rate of the composite material is still low(1.44 ×10−5 mm3/Nm).

Label-free and early detection of HER2 breast cancer biomarker based on UiO-66-NH2 modified gold chip (Au/UiO-66-NH2) using surface plasmon resonance technique

Breast cancer is one of the leading causes of cancer death for women worldwide. This cancer occurs when the breast cells grow abnormally and spread uncontrolled. Although there are several treatments, including chemotherapy, radiation, and surgery in a clinical setting to cure cancer, these treatments cannot provide a 100 % success rate in eliminating cancer, mainly due to the late diagnosis of the disease. Thus, early detection is the key to provide better breast cancer treatment and a higher chance for survival. Herein, simple and sensitive biosensor by combining the emerging multifunctional materials of metal-organic frameworks (MOFs) with a label-free surface plasmon resonance (SPR) instrument was developed. Two types of stable Zirconium (Zr)-based MOFs, namely Zr benzene dicarboxylic acid (Zr-BDC or UiO-66) and Zr 2-amino-benzene dicarboxylic acid (Zr-BDC-NH2 or UiO-66-NH2) were synthesized using the solvothermal method. X-ray diffractometer (XRD) and fourier transform infra red (FTIR) confirmed the formation of the prepared UiO-66 and UiO-66-NH2. The electron microscopy and nitrogen adsorption desorption isotherm characterization reveal that the MOFs have a cubic crystal structure with a high surface area of 672.4 m2/g for UiO-66 and 286.1 m2/g for UiO-66-NH2. The MOFs are then functionalized on the SPR gold (Au) chip surface to serve as a matrix of Human Epidermal Growth Factor Receptor 2(HER2) antibody receptors. Interestingly, the functionalized SPR biosensors could detect the HER2 protein target with high sensitivity in the ng/mL range and a remarkable detection limit, which is 0.457 ng/mL for UiO-66-NH2. This LOD value is significantly lower than clinical concentrations of HER2 proteins in human blood (∼15 ng/mL), which indicate that the biosensor can offer the capability of early detection test.

A Hierarchical Classification Method for High-accuracy Instruction Disassembly with Near-field EM Measurements

Electromagnetic (EM) fields have been extensively studied as potent side-channel tools for testing the security of hardware implementations. In this work, a low-cost side-channel disassembler that uses fine-grained EM signals to predict a program's execution trace with high accuracy is proposed. Unlike conventional side-channel disassemblers, the proposed disassembler does not require extensive randomized instantiations of instructions to profile them, instead relying on leakage-model-informed sub-sampling of potential architectural states resulting from instruction execution, which is further augmented by using a structured hierarchical approach. The proposed disassembler consists of two phases: (i) In the feature-selection phase, signals are collected with a relatively small EM probe, performing high-resolution scans near the chip surface, as profiling codes are executed. The measured signals from the numerous probe configurations are compiled into a hierarchical database by storing the min-max envelopes of the probed EM fields and differential signals derived from them, a novel dimension that increases the potency of the analysis. The envelope-to-envelope distances are evaluated throughout the hierarchy to identify optimal measurement configurations that maximize the distance between each pair of instruction classes. (ii) In the classification phase, signals measured for unknown instructions using optimal measurement configurations identified in the first phase are compared to the envelopes stored in the database to perform binary classification with majority voting, identifying candidate instruction classes at each hierarchical stage. Both phases of the disassembler rely on a four-stage hierarchical grouping of instructions by their length, size, operands, and functions. The proposed disassembler is shown to recover ∼97–99% of instructions from several test and application benchmark programs executed on the AT89S51 microcontroller.

Biohybrid Photonic Platform for Subcellular Stimulation and Readout of In Vitro Neurons.

Targeted manipulation of neural activity via light has become an indispensable tool for gaining insights into the intricate processes governing single neurons and complex neural networks. To shed light onto the underlying interaction mechanisms, it is crucial to achieve precise control of individual neural activity, as well as a spatial read-out resolution on the nanoscale. Here, a versatile photonic platform with subcellular resolution for stimulation and monitoring of in-vitro neurons is demonstrated. Low-loss photonic waveguides are fabricated on glass substrates using nanoimprint lithography and featuring a loss of only -0.9±0.2dBcm-1 at 489nm and are combined with optical fiber-based waveguide-access and backside total internal reflection fluorescence microscopy. Neurons are grown on the bio-functionalized photonic chip surface and, expressing the light-sensitive ion channel Channelrhodopsin-2, are stimulated within the evanescent field penetration depth of 57nm of the biocompatible waveguides. The versatility and cost-efficiency of the platform, along with the possible subcellular resolution, enable tailor-made investigations of neural interaction dynamics with defined spatial control and high throughput.

Characterization of the current crowding effect on chip surface using a quantum wide-field microscope

Characterization of the current crowding effect on chip surface using a quantum wide-field microscope

Low-Power Fully Integrated 256-Channel Nanowire Electrode-on-Chip Neural Interface for Intracellular Electrophysiology.

Intracellular electrophysiology, a vital and versatile technique in cellular neuroscience, is typically conducted using the patch-clamp method. Despite its effectiveness, this method poses challenges due to its complexity and low throughput. The pursuit of multi-channel parallel neural intracellular recording has been a long-standing goal, yet achieving reliable and consistent scaling has been elusive because of several technological barriers. In this work, we introduce a micropower integrated circuit, optimized for scalable, high-throughput in vitro intrinsically intracellular electrophysiology. This system is capable of simultaneous recording and stimulation, implementing all essential functions such as signal amplification, acquisition, and control, with a direct interface to electrodes integrated on the chip. The electrophysiology system-on-chip (eSoC), fabricated in 180nm CMOS, measures 2.236 mm × 2.236 mm. It contains four 8 × 8 arrays of nanowire electrodes, each with a 50 μm pitch, placed over the top-metal layer on the chip surface, totaling 256 channels. Each channel has a power consumption of 0.47 μW, suitable for current stimulation and voltage recording, and covers 80 dB adjustable range at a sampling rate of 25 kHz. Experimental recordings with the eSoC from cultured neurons in vitro validate its functionality in accurately resolving chemically induced multi-unit intracellular electrical activity.

Gallium oxide cantilevered thin film-based solar-blind photodetector and its arc detection applications

The performance of gallium oxide (Ga<sub>2</sub>O<sub>3</sub>) thin film detector based on metal-semiconductor-metal (MSM) is highly dependent on the uniformity of the Ga<sub>2</sub>O<sub>3</sub> thin film, and the manufacturing process is quite sophisticated, which poses a challenge for the scale-up and mass production of thin film photodetectors. In this work, an MSM Ga<sub>2</sub>O<sub>3</sub> thin film solar-blind photodetector with five-finger interdigital electrodes is fabricated by physically depositing Ga<sub>2</sub>O<sub>3</sub> thin film on the surface of a mass-produced cantilevered thin film chip. Through the microelectromechanical system (MEMS) process, the cantilever electrode structure is prepared, which protects the internal circuit and the integrity of the thin film. The Ga<sub>2</sub>O<sub>3</sub> thin film treated by argon plasma at a low temperature is amorphous, but the photodetector still possesses considerable ultraviolet detection performance. At a bias voltage of 18 V, it approaches the detection performance of crystalline Ga<sub>2</sub>O<sub>3</sub> thin film, with a detectivity of 7.9×10<sup>10</sup> Jones, an external quantum efficiency of 1779%, rise time and decay time of 1.22 s and 0.24 s, respectively. Moreover, a system of arc detection is built in outdoor environments. Without any optical focusing system, this photodetector achieves sensitive detection of pulsed arc in an outdoor sunlight environments. For pulsed arcs with an output voltage of 100 kV, the photodetector is capable of sensitive detection at a distance of 25 cm. Besides, the maximum detection distance of 155 cm indicates that the photodetector will have a favorable potential application value in the field of solar-blind detection. This work develops a sensitive functional thin film deposition technology based on the cantilever electrode structure fabricated by the MEMS process, which avoids the influence of the large-area uniformity of the functional thin film on the etching circuit. It provides a new technical approach and process route for preparing MSM photodetectors.

Recent Progress of Electromagnetic Field Characterization of Chip Surface

The rapid development of chip manufacturing technology has increased the demand for precise characterization techniques. The characterization technique of the physical field on the surface of a chip is crucial for analyzing chip failures and diagnosing faults. In this review, the latest advancements based on different measurement mechanisms are analyzed and summarized for the electromagnetic field characterization of the chip surfaces. In addition, their advantages and limitations are discussed. Finally, prospects for future development are presented.

Numerical modelling and analysis of porous surface-enhanced jet cooling using copper inverse opals in single-phase flow

Abstract In this study, we propose a novel cooling scheme that utilizes copper inverse opals (CIOs) for surface enhancement in a single-phase impingement jet cooling system. We perform computational fluid dynamics simulations to evaluate the cooling performance of the CIO jet coolers. Our modelling results indicate that the proposed CIO-coated cooler can significantly reduce the average temperature and improve the temperature uniformity across the entire chip surface. The average Nusselt number of the CIO-coated cooler can reach up to 2.8 times that of the flat surface jet cooler. However, the porous structure of the CIO-coated cooler increases the total pressure drop. To determine designs with high cooling performance and low energy consumption, we investigate two crucial design factors, namely the inlet velocity and the nozzle-to-CIO distance. Our analysis reveals that increasing the inlet velocity further enhances the heat transfer, but at the expense of high pressure drop. On the other hand, a larger nozzle-to-CIO distance results in a lower pressure drop but also reduces the heat transfer coefficient. The effects of nozzle-to-CIO distance are further understood by studying the flow resistance network. Furthermore, we present a reduced-order model that accurately captures the thermofluidic characteristics of the proposed design.

High-Speed and Accurate Cascade Detection Method for Chip Surface Defects

High-Speed and Accurate Cascade Detection Method for Chip Surface Defects

MS Identification of Blood Plasma Proteins Concentrated on a Photocrosslinker-Modified Surface.

This work demonstrates the use of a modified mica to concentrate proteins, which is required for proteomic profiling of blood plasma by mass spectrometry (MS). The surface of mica substrates, which are routinely used in atomic force microscopy (AFM), was modified with a photocrosslinker to allow "irreversible" binding of proteins via covalent bond formation. This modified substrate was called the AFM chip. This study aimed to determine the role of the surface and crosslinker in the efficient concentration of various types of proteins in plasma over a wide concentration range. The substrate surface was modified with a 4-benzoylbenzoic acid N-succinimidyl ester (SuccBB) photocrosslinker, activated by UV irradiation. AFM chips were incubated with plasma samples from a healthy volunteer at various dilution ratios (102X, 104X, and 106X). Control experiments were performed without UV irradiation to evaluate the contribution of physical protein adsorption to the concentration efficiency. AFM imaging confirmed the presence of protein layers on the chip surface after incubation with the samples. MS analysis of different samples indicated that the proteomic profile of the AFM-visualized layers contained common and unique proteins. In the working series of experiments, 228 proteins were identified on the chip surface for all samples, and 21 proteins were not identified in the control series. In the control series, a total of 220 proteins were identified on the chip surface, seven of which were not found in the working series. In plasma samples at various dilution ratios, a total of 146 proteins were identified without the concentration step, while 17 proteins were not detected in the series using AFM chips. The introduction of a concentration step using AFM chips allowed us to identify more proteins than in plasma samples without this step. We found that AFM chips with a modified surface facilitate the efficient concentration of proteins owing to the adsorption factor and the formation of covalent bonds between the proteins and the chip surface. The results of our study can be applied in the development of highly sensitive analytical systems for determining the complete composition of the plasma proteome.

Towards optical MAS magnetic resonance using optical traps

Higher magic angle spinning (MAS) frequencies than currently available are desirable to improve spectral resolution in NMR and EPR systems. While conventional strategies employ pneumatic spinning limited by fluid dynamics, this paper demonstrates the development of an optical spinning technique in which vacuum quality dictates the maximum achievable spinning frequency. Using optical traps, we levitated a range of micron-sized samples. Under vacuum we achieved optical rotation of a single ∼10 μm diameter particle of vaterite at several mbar up to hundreds of Hz and of 20 μm diameter SiO2 particles at ≤10−2 mbar at several kHz. At ambient conditions, we optically levitated γ-irradiated alanine particles of 20–50 μm diameter. Additionally, using a single chip EPR detector operating at 11 GHz, we measured the EPR spectrum for a 30 μm γ-irradiated alanine particle in contact with the chip surface (i.e., without optical levitation) in a single scan lasting 92 s. These observations suggest that a γ-irradiated alanine particle having a diameter in the order of 30 μm is a promising candidate for our aim of demonstrating the first magnetic resonance experiment on optically levitated samples. Furthermore, we discuss strategies, limitations, and the potential of implementing MAS with optical traps for NMR and EPR.

Gold nanoparticle-powered screening of membrane protein-specific lipids from complex lipid mixtures

Membrane proteins (MPs) are affected by binding of specific lipids. We previously developed a methodology for systematically analyzing MP-lipid interactions leveraging surface plasmon resonance (SPR). In this method, the gold sensor chip surface was modified with a self-assembled monolayer (SAM), which allowed for a larger amount of MP-immobilization. However, the laborious lipid purification step remained a bottleneck. To address this issue, a new strategy has been developed utilizing gold nanoparticles (AuNPs) instead of the gold sensor chip. AuNPs were coated with SAM, on which MP was covalently anchored. The MP-immobilized AuNPs were mixed with a lipid mixture, and the recovered lipids were quantified by LC–MS. Bacteriorhodopsin (bR) was used as an MP to demonstrate this concept. We optimized immobilization conditions and confirmed the efficient immobilization of bR by dynamic light scattering and electron micrographs. Washing conditions for pulldown experiments were optimized to efficiently remove non-specific lipids. A new binding index was introduced to qualitatively reproduce the known affinity of lipids for bR. Consequently, the low-abundant and least-studied lipid S-TeGD was identified as a candidate for bR-specific lipids. This technique can skip the laborious lipid purification process, accelerating the screening of MP-specific lipids from complex lipid mixtures.

Multifunctional DNA scaffold mediated gap plasmon resonance: Application to sensitive PD-L1 sensor

The introduction of noble metal nanoparticles with good LSPR characteristics can greatly improve the sensitivity of SPR through resonance coupling effect. The plasma resonance response and optical properties of film coupling nanoparticle systems largely depends on the ingenious design of gap structures. Nucleic acid nanostructures have good stability, flexibility, and high biocompatibility, making them ideal materials for gap construction. 2D MOF (Cu-Tcpp) has a large conjugated surface similar to graphene, which can provide a stable substrate for the directional fixation of nucleic acid nanostructures. However, research on gap coupling plasmon based on nucleic acid nanostructures and 2D MOF is still rarely reported. By integrating the advantages of Cu-Tcpp assembled film and DNA tetrahedron immobilization, a nano gap with porous scaffold structure between the gold film and gold nanorod was build. The rigidity of DNA tetrahedron can precisely control the gap size, and its unique programmability allows us to give the coupling structure greater flexibility through the design of nucleic acid chain. The experimental results and FDTD simulation show that the film coupling nanoparticle systems constructed with DNA tetrahedrons greatly enhance the electric field strength near the chip surface and effectively improve the sensitivity of SPR. This research shows the huge potential of nucleic acid nanomaterials in the construction of SPR chip surface microstructures.

Acoustofluidics-assisted strategy of zinc oxide nanoarrays for enhancement of phase-change chip cooling

Enhancing flow boiling in microchannel via surface modification is crucial for addressing the energy consumption challenges posed by high-power compact electronic devices. However, improving boiling heat transfer performance with well-defined nanostructured surfaces in a limited space remains a challenge. Herein, we present a simple and straightforward acoustofluidics strategy for stable, controllable, and efficient fabricates of functional Zinc oxide (ZnO) nanoarray silicon chip surface with excellent phase change cooling performance. The intentionally designed flower-like sharp-edge structure integrated acoustic has been experimentally and numerically verified for its enhanced mass transfer mixing. The resulting ZnO nanoarray-coated chip with customizable lengths, densities, and morphology is implemented by simple reactor parameter adjustment. Excellent boiling heat transfer performance is obtained on this surface, giving priority to nucleation (superheat≈ 4 °C), low energy consumption (≤3.2 kPa) and simultaneously enhancing the critical heat flux (CHF) and heat-transfer coefficient (HTC) by up to 70.8 % and 107.5 %, respectively, compared with a smooth chip surface. In situ observation and analysis of the wicking of the nanoarray and nucleation, growth, and departure of the bubbles reflect that ZnO nanoarray promotes the phase change heat exchange process by the large number of nucleation sites and ultrafast liquid re-wetting. These findings not only provide important guidelines for the precise control and rational design of functional nanomaterials, but also provide new insights for embedded cooling and significant energy savings on power devices.

The effect of using wood chips exposed to mold fungi on the properties of chipboard

ABSTRACT Considering that commonly occurring mold fungi have the ability to develop very quickly on the surface of wood chips and the ongoing studies on finding the ways to use degraded wood, the conducted research was aimed at determining the physical and mechanical properties of chipboards containing various amounts (0%, 30%, 50%, and 70%) of pine chips inoculated with Aspergillus and Penicillium fungi, varying in a degree of infestation. The inoculation did not cause significant changes in the chemical composition of chips; however, it caused a decrease in the pH of their surface after nine-week incubation. While using them, lowered pH caused a deterioration in the mechanical characteristics of the boards and increased their thickness swelling and water absorption. Moreover, no changes in density, formaldehyde content and decomposition by brown-rot fungi were found. Overall, it was concluded that the addition of partially infested chips did not significantly affect any properties of the board; however, in the case of complete overgrowth, the amount of degraded chips should be less than 30%.

Selection of Aptamers for Use as Molecular Probes in AFM Detection of Proteins.

Currently, there is great interest in the development of highly sensitive bioanalytical systems for diagnosing diseases at an early stage, when pathological biomarkers are present in biological fluids at low concentrations and there are no clinical manifestations. A promising direction is the use of molecular detectors-highly sensitive devices that detect signals from single biomacromolecules. A typical detector in this class is the atomic force microscope (AFM). The high sensitivity of an AFM-based bioanalysis system is determined by the size of the sensing element of an atomic force microscope-the cantilever-the radius of the curvature of which is comparable to that of a biomolecule. Biospecific molecular probe-target interactions are used to ensure detection system specificity. Antibodies, aptamers, synthetic antibodies, and peptides can be used as molecular probes. This study has demonstrated the possibility of using aptamers as molecular probes for AFM-based detection of the ovarian cancer biomarker CA125. Antigen detection in a nanomolar solution was carried out using AFM chips with immobilized aptamers, commercially available or synthesized based on sequences from open sources. Both aptamer types can be used for antigen detection, but the availability of sequence information enables additional modeling of the aptamer structure with allowance for modifications necessary for immobilization of the aptamer on an AFM chip surface. Information on the structure and oligomeric composition of aptamers in the solution was acquired by combining small-angle X-ray scattering and molecular modeling. Modeling enabled pre-selection, before the experimental stage, of aptamers for use as surface-immobilized molecular probes.

Characterization of magnetic field for loading, trapping and transferring cold atom close to the atom chip’s surface

Atom chips provide flexible technologies for implementing modern concepts in quantum optics, quantum measurement, and quantum information processing. Atom chips are miniature devices that confine, control and manipulate cold atoms using electric, magnetic, and light fields. Due to the shrinkage of scale, arbitrary magnetic traps can be generated from current sources outside the chip’s vacuum compartment, in contrast to a traditional setup. This makes it easy to change trap configurations in an experiment that involves rapid prototyping of quantum states and quantum trajectory designs in free space. In this work, we show relevant parameters needed for transferring a cold atom cloud at the recoil limit from a magneto-optical trap (MOT) to an area close to the atom chip. To create a movable magnetic potential for this transfer, we used the MOT coils and an additional pair of coils in an anti-Helmholtz configuration. The properties of the movable potential were obtained by performing the Computer Simulation Tool (CST EM Studio suite®). Furthermore, an appropriate magnetic trap on a chip is developed, based on the simulation from COMSOL Multiphysics. We used a magnetic field gradient of around 20 G/cm to transport the cold atom with a distance over 20 mm with a temperature gain below 100 micro-Kelvin. The simulation results are based on an atom chip with a size of 2×2 cm2 and a copper wire thickness of 2 mm. The atom chip consists of Z, U and I-shaped wires that generate a quadrupole magnetic field. The resulting field minimum can be made at least 7 mm away from the chip surface.

Computational analysis of protein synthesis, diffusion, and binding in compartmental biochips

Protein complex assembly facilitates the combination of individual protein subunits into functional entities, and thus plays a crucial role in biology and biotechnology. Recently, we developed quasi-twodimensional, silicon-based compartmental biochips that are designed to study and administer the synthesis and assembly of protein complexes. At these biochips, individual protein subunits are synthesized from locally confined high-density DNA brushes and are captured on the chip surface by molecular traps. Here, we investigate single-gene versions of our quasi-twodimensional synthesis systems and introduce the trap-binding efficiency to characterize their performance. We show by mathematical and computational modeling how a finite trap density determines the dynamics of protein-trap binding and identify three distinct regimes of the trap-binding efficiency. We systematically study how protein-trap binding is governed by the system’s three key parameters, which are the synthesis rate, the diffusion constant and the trap-binding affinity of the expressed protein. In addition, we describe how spatially differential patterns of traps modulate the protein-trap binding dynamics. In this way, we extend the theoretical knowledge base for synthesis, diffusion, and binding in compartmental systems, which helps to achieve better control of directed molecular self-assembly required for the fabrication of nanomachines for synthetic biology applications or nanotechnological purposes.

Thermo-hydraulic performance evaluation of radial tree-branching microchannel heat sinks for electronic cooling applications

The fractal-like tree-branching microchannel heat sink (TB-MCHS) can be a potential solution for the cooling of electronic devices due to its ability to dissipate high heat flux and maintain uniform temperature distribution on the chip surface while requiring lower pumping power compared with the conventional TB-MCHS with constant channel depth. This work proposed a novel design of radial TB-MCHS with continuously varying, either increasing or decreasing, channel depth from the centre inlet to the radial outlet, resulting in different fluid volumes in channels compared to the conventional constant channel height TB-MCHS. A three-dimensional numerical model is developed and validated with in-house experimental results to study the thermo-hydrodynamic performance and entropy generation of the TB-MCHSs. The results reveal that the proposed designs of TB-MCHS with different fluid volumes negligibly enhance the heat transfer coefficient and significantly reduce the pressure drop and pumping power requirement compared to that of constant fluid volumes. The TB-MCHS with continuously increasing channel depth (TB-MCHS-DIV) exhibits the lowest pressure drop, thermal resistance, substrate temperature, and entropy generation among all the designs of TB-MCHS considered. However, the TB-MCHS-DIV shows the lowest convective heat transfer coefficient and higher coefficient of performance (COP) and heat removal rate than the TB-MCHS-ST. Further, it is found that the TB-MCHS-DIV can be performed better than TB-MCHS-CON and TB-MCHS-ST at constant pumping power.