Antioxidant Vitamin Supplementation Research Articles

#1526 Effects of dietary intervention on diabetic nephropathy: an umbrella review of systematic review and meta-analysis of randomized controlled trials

Abstract Background and Aims To evaluate the quality of evidence, potential biases, and validity of all available studies on dietary intervention and diabetic nephropathy (DN). Method We conducted an umbrella review of existing meta-analyses of randomized controlled trials (RCTs) that focused on the effects of dietary intervention on DN. Literature searching was performed via PubMed, Embase, Web of Science, Cochrane Database of Systematic Reviews, and hand searching of the reference lists. Systematic reviews and meta-analyses of RCTs that evaluated the effects of any dietary intervention on clinical outcomes of DN were included. Two reviewers evaluated the methodological quality of the included articles through AMSTAR score. In addition, according to the Grading of Recommendations, Assessment, Development and Evaluation (GRADE), evidence of each outcome was evaluated and graded as “high”, “moderate”, “low” or “very low” quality to draw conclusions. Additionally, we classified evidence of outcomes into 4 categories (class I to IV and non-significant (NS)). Results We identified 36 meta-analyses of RCTs and 55 clinical outcomes of DN from 395 unique articles (Fig. 1). Moderate quality evidence suggested that probiotics supplementation could significantly improve blood urea nitrogen (BUN), total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) in DN patients. Low quality evidence indicated that probiotics supplementation significantly improved serum creatinine, urinary albumin creatinine ratio (UACR), fasting blood glucose (FBG), HbA1c and high-density lipoprotein cholesterol (HDL-C) in DN patients. In addition, low quality evidence suggested that a salt restriction diet could significantly improve creatinine clearance rate (CrCl) in patients with DN. Low quality evidence suggested that vitamin D supplementation could significantly improve UACR in patients with DN. Besides, low quality evidence indicated that soy isoflavone supplementation could significantly improve BUN, FBG, TC, triglycerides (TG) and LDL-C in patients with DN. Furthermore, low quality evidence suggested that coenzyme Q10 supplementation could significantly improve HbA1c, TC and HDL-C in patients with DN, and dietary polyphenols also significantly improved HbA1c in patients with DN. Finally, low quality evidence suggested supplementation of antioxidant vitamins could significantly improve serum creatinine, systolic blood pressure, and HbA1c in patients with DN. The remaining outcomes were rated as very low quality due to significant risk of bias, significant heterogeneity, imprecision, and publication bias. Given the small sample size, all significantly associated outcomes were evaluated as class IV evidence (Fig. 2). Conclusion Moderate to low evidence suggests that supplementation with probiotics, vitamin D, soy isoflavones, coenzyme Q10, dietary polyphenols, antioxidant vitamins, and salt-restricted diets may significantly improve clinical outcomes in patients with DN.

Effects of antioxidant vitamin supplementation on sports performance, endurance and strength performance: a systematic review and meta-analysis

Effects of antioxidant vitamin supplementation on sports performance, endurance and strength performance: a systematic review and meta-analysis

Antioxidant Vitamins (Vit C and E) Protect the Kidney from Glyphosate- Exposed Renal Damage in Experimental Rats - A Molecular Study

Glyphosate is a widely used herbicide and the active ingredient in Monsanto's Roundup, among other herbicide formulations. Vitamin C (ascorbic acid) and vitamin E (tocopherol) are both antioxidants that play important roles in reducing oxidative DNA damage and protecting cells from the harmful effects of free radicals. The study aimed to analyse the effect of antioxidant vitamins (vitamins C and E) on the expression of IGF-1 and KIM-1 molecules in the kidneys of glyphosate-exposed rats. Adult male Albino Wistar rats were grouped into 3. Group I: Control; Group II: Glyphosate treated (100mg); Group III: Glyphosate+ vitamin E & C treated. Serum, insulin, and mRNA expression of insulin-like growth factor (IGF-1) and kidney injury molecules (KIM-1) mRNA expression by RT-PCR. Serum insulin was measured by the ELISA method. Glyphosate-exposed rats developed hyperglycaemia and hyper compared to control but antioxidant vitamins (Vitamin C and E) supplementation, reduced the glyphosate mediate increased fasting blood glucose and insulin near to that of the control level. Similarly, mRNA expression of both IGF-1 and KIM-1 were also significantly altered due to glyphosate induction whose effects were found to be reduced when vitamins C and E were treated. It is concluded that glyphosate exposure causes renal damage and develops diabetic nephropathy by modulating the expression of IGF-1 and KIM-1 molecules in the kidney. Vitamin C and E potentially were able to reduce the detrimental changes caused by glyphosate. Hence, vitamin C and E supplements could be considered therapeutic drugs for diabetic nephropathy.

Impact on methemoglobin and certain oxidative stress markers in diabetic and non-diabetic Hansen’s disease patients with dapsone treatment

Introduction: Hansen’s (leprosy) disease is a worldwide healthcare problem, caused by Mycobacterium leprae. Dapsone (a synthetic sulfone), is an antibacterial agent, has both anti-inflammatory and immunomodulatory properties for the treatment of all forms of leprosy. The goal of the study is to evaluate the effect of dapsone on HbA1C, methemoglobin and certain oxidative stress markers in young patients suffering with Hansen’s disease with and without diabetes.
 
 Methodology: The study was conducted on 70 subjects in age group of 20-45 years. These subjects include 20 healthy subjects (Group 1); 35 non-diabetic Hansen’s patients on dapsone (100mg/day) treatment (Group 2) and 15 diabetic Hansen’s disease on dapsone (100 mg/day) (Group 3). The fasting blood samples were drawn for the evaluation of methemoglobin, HbA1C, malondialdehyde, reduced glutathione and total antioxidant activity from all the groups.
 
 Results: A significant increase in methemoglobin and malondialdehyde was noted in Group 2 and 3 patients with reference to healthy control subjects (Group 1) while the levels of reduced glutathione, HbA1C and total antioxidant activity were notably reduced in investigated Group 2 and 3 compared to Group 1. A similar trend of significant increase in methemoglobin and malondialdehyde levels in Group 3 with respect to Group 2 and significant fall in glutathione and total antioxidant activity and nominal fall in HbA1C was recorded in Group 3 with respect to Group 2.
 
 Conclusion: Aforesaid observations suggested that oxidative stress induced in diabetic and non-diabetic Hansen’s disease patients treated with dapsone due to alteration in Met. Hb, MDA, reduced glutathione and total antioxidant activity levels might be accountable for the etiology of various diseases like cancer, arthritis, and cardiovascular events. A comprehensive study with clinical trials inclusive of complete oxidative stress markers, antioxidant enzymes, pattern of dietary intake with antioxidant vitamin supplements alone and in combination might be beneficial.

Effect of Antioxidants on oxidative damage, and hematological and psychological parameters in cement-exposed workers

Some studies suggest that exposure to cement dust can cause oxidative stress, and have adverse effects on liver function, and hematological and psychological parameters. This study aimed to assess the effect of antioxidant vitamin supplementation on indices of oxidative stress, biochemical indicators, and psychological factors among cement plant workers. A total of 80 subjects (workers and non-exposed office employees) were divided into two groups. The intervention group that took vitamin E (400 IU)+ C (500 mg) and the control group that took placebo, for 60 days. The levels of malondialdehyde (MDA), total antioxidant capacity (TAC), superoxide dismutase (SOD) and catalase (CAT) were measured in plasma, and hematological parameters were also recorded before and after the intervention. A significant decrease in the mean level of MDA, SOD and CAT, alkaline phosphatase (ALP), alanine transaminase (ALT) and aspartate transaminase (AST) were detected. On the other hand, a significant increase in the level of TAC was observed in the workers, after the intervention. Depression and anxiety also decreased significantly after the intervention. Vitamin E and C were probably effective in reducing oxidative stress and improving mental health and liver function especially among the workers.

Alzheimer's Disease Neuropathologic Change and Vitamin Supplement Use Decades Earlier: The 90+ Study.

Alzheimer's disease (AD) is the most common cause of dementia. AD neuropathologic change (ADNC) likely begins decades before clinical manifestations. One mechanism implicated in AD is oxidative stress. We explored the potential association of ADNC with antioxidant vitamin supplements taken about 30 years before death. The 264 brain-autopsied participants were part of The 90+ Study, a longitudinal study of aging among people aged 90+ years, and originally members of the Leisure World Cohort Study, a population-based health study established in the 1980s. Intake of supplemental vitamins A, C, and E was collected by the Leisure World Cohort Study about 30 years before ADNC assessment. Odds ratios of ADNC (intermediate/high vs. none/low) for vitamin intake were estimated using logistic regression. The adjusted odds ratio (95% CI) of ADNC was 0.52 (0.29-0.92) for vitamin E supplements and 0.51 (0.27-0.93) for vitamin C supplements. Supplemental vitamin E intake was the first variable, after education, to enter the stepwise model. Intake of vitamin A or C did not improve the model fit. The observed association of ADNC and supplemental vitamin E intake decades earlier suggests a beneficial effect and supports further investigation into a nutritional approach to preventing AD with vitamin supplementation.

CIGARETTE SMOKE INTENSITY AND AN AMELIORATIVE ROLE OF VITAMIN C ON BLOOD CELL COUNTS IN ALBINO RATS

In the present study, cigarette smoke exposure shows significant alterations on blood parametersviz., Total Erythrocyte counts (TEC), Haemoglobin concentration (Hb.con.), packed cell volume(PCV), Erythrocyte Sedimentation rate (ESR), Total leukocyte counts and platelets count in albinorats. These changes may be due to the oxidative stress and inflammatory responses induced bycigarette smoke on haematopoietic system. Cigarette smoke consists of carbon monoxide andnicotinamide, which are toxic gases having the capacity to binds with the haemoglobin in redblood cells and interferes with the body’s ability to supply inadequate oxygen to the tissues thatacts as a stimulus for marrow production in the result of hypoxic polycythemia and also inducedan irritated action of tobacco smoke on the respiratory tract which causes leukocytosis in albinorats. While after supplementation of antioxidant vitamin C, which reduces the toxicity generatedby cigarette smoke to a maximum extent. This is due to the antioxidant defence mechanism ofvitamin C, which mitigates the toxic actionof cigarette smoke in albino rats.

Antioxidant vitamin supplementation on muscle adaptations to resistance training: A double-blind, randomized controlled trial

ObjectivesThe aim of this study was to examine whether antioxidant vitamin supplementation with vitamin C (VitC) and vitamin E (VitE) affects the hypertrophic and functional adaptations to resistance training in trained men. MethodsThis was a double-blind, randomized controlled trial in which participants were supplemented daily with VitC and VitE ( n = 12) or placebo ( n = 11) while completing a 10-wk resistance training program accompanied by a dietary intervention (300 kcal surplus and adequate protein intake) designed to optimize hypertrophy. Body composition (dual-energy x-ray absorptiometry), handgrip strength, and one-repetition maximum (1-RM), maximal force (F0), velocity (V0), and power (Pmax) were measured in bench press (BP) and squat (SQ) tests conducted before and after the intervention. To detect between-group differences, multiple-mixed analysis of variance, standardized differences, and qualitative differences were estimated. Relative changes within each group were assessed using a paired Student's t test. ResultsIn both groups, similar improvements were produced in BP 1-RM , SQ 1-RM SQ, and BP F0 (P < 0.05) after the resistance training program. A small effect size was observed for BP 1-RM (d = 0.53), BP F0 (d = 0.48), and SQ 1-RM (d = –0.39), but not for SQ F0 (d = 0.03). Dominant handgrip strength was significantly increased only in the placebo group (P < 0.05). According to body composition data, a significant increase was produced in upper body fat-free mass soft tissue (FFMST; P < 0.05) in the placebo group, whereas neither total nor segmental FFMST was increased in the vitamin group. Small intervention effect sizes were observed for upper body FFSMT (d = 0.32), non-dominant and dominant leg FFMST (d = –0.39; d = –0.42). Although a significant increase in total body fat was observed in both groups (P < 0.05) only the placebo group showed an increase in visceral adipose tissue (P < 0.05), showing a substantial intervention effect (d = 0.85). ConclusionsThe data indicated that, although VitC/VitE supplementation seemed to blunt upper body strength and hypertrophy adaptations to resistance training, it could also mitigate gains in visceral adipose tissue elicited by an energy surplus.

КОРМОВЫЕ ДОБАВКИ С СОРБЦИОННЫМИ И АНТИОКСИДАНТНЫМИ СВОЙСТВАМИ ДЛЯ КОРРЕКЦИИ ИММУННОГО СТАТУСА И УВЕЛИЧЕНИЯ ПРОДУКТИВНОСТИ КРУПНОГО РОГАТОГО СКОТА ПРИ ОБЪЁМИСТОМ ТИПЕ КОРМЛЕНИЯ

The article presents results of studies on correction of silage and haylage rations of young fattening cattle and lactating cows by using sorbing, sorption- probiotic and antioxidant vitamin and mineral supplements. The application of such feed additives as Koretron and Biokoretron-forte in hay rations of fattening cattle in the amount of 1.1 % of its dry matter stimulates protein-synthesizing liver function, which is shown by concentration increase of total protein in blood serum of young animals in the experimental groups compared to the control (by 3, 97 and 5.01 %) albumin (6.21 and 9.34 %) and alpha globulins (9.26 and 16.74 %), concentration increase of immunoglobulins of A, M, G classes, all these parametres are reflected in an increase of average daily gains by 11.75 and 14.73 %. Adding the antioxidant vitamin and mineral supplement Lipovitam-beta to silage rations of cows at a dose of 4 g every 5 days increases their productivity by 7.41 %, which is also confirmed by an increase of serum protein concentration in their blood (by 2.04 %), besides, there is a significant increase of globulins by 3.52 % in the fractional spectrum, the fraction increase of gamma globulins (by 4.69 %), which carry the main load for formation of antibodies, is especially pronounced. The application of Lipovitam - beta has an immunostimulating effect during the period of late pregnancy of cows (7-8 months of pregnancy), which is confirmed by a significant increase of immunoglobulins of A, M and G classes in blood serum, by 8.38 %; 9.37 % and 13.01 %, respectively. Cow reproduction rates are improved

الإجهاد التأکسدى ومضادات الأکسدة للفئران المصابة بالسکرى والتى تم تغذیتها على بعض مستخلصات الأجزاء النیاتیة Oxidative stress and antioxidant defense systems status in diabetic rats feeding some selected plant partexctract

Abstract:Oxidative stress plays a pathological role in the development of various diseases including diabetes. Systemic oxidative stress results from an imbalance between oxidants derivatives production and antioxidants defenses. The present study aims to investigate the oxidative stress and antioxidant defense system status in diabetic rats feeding some selected plant parts such okra fruits water extract (OFWE), white mulberry leaves water extract (MLWE), celery leaves water extract (CLWE) and their Mixture. Treatment of animals with alloxancaused a significant increased (p≤0.05) in serum glucose concentration by the ratio 136.51% compared to normal animals (negative control group). Supplementation of the rat diets with 2% w/w by OFWE, MLWE, CLWE and their mixture decreased this elevation value compared to normal controls and recorded 57.35, 38.69, 52.27 and 35.48%, respectively. Also, consumption of OFWE, MLWE, CLWE and their mixture induced significant improvements on plasma glutathione fractions concentration through increasing the reduced glutathione (GSH) and oxidized glutathione (GSSG)compared to normal controls by the ratio of -15.31; -11.22, -14.29 and -7.14%; and -15.31, -11.22, -14.29 and -7.14%, respectively. The opposite direction was observed with the biological oxidants includingthiobarbituric acid reactive substances (TBARS) and nitric oxides (NO2 and NO3).In conclusion,the present data support the benefits of dietary modification, including bioactive compounds and antioxidant vitamins supplementation, in alleviating oxidative stress associated diabetes.

Kronik Böbrek Yetmezliğinde Antioksidan Vitaminlerin İnflamasyon ve Oksidatif Stres Üzerine Etkisi

Kronik böbrek yetmezliği (KBY), inflamasyonun ve oksidatif stresin sıklıkla eşlik ettiği bir süreç olarak bilinmektedir. Kronik böbrek yetmezliği hastalarında artan oksidatif stres düzeyleri, azalmış antioksidan savunma ve ortaya çıkan kronik inflamasyon kardiyovasküler hastalıklar başta olmak üzere birçok sağlık sorunu için risk faktörü olabilmektedir. Bu nedenle hastalarda antioksidan tedavinin uygun kullanımı oldukça önem taşımaktadır. Antioksidan vitaminlerin sürece etkisi değerlendirildiğinde, KBY hastalarında retinol bağlayıcı protein katabolizmasındaki azalmalar nedeniyle serum A vitamini konsantrasyonlarının sıklıkla yüksek olduğu düşünülmektedir. Buna bağlı olarak KBY hastalarına ek A vitamini suplemantasyonu önerilmemektedir. Diğer bir antioksidan olan C vitamininin, prooksidan etki gösterebilme ve oksalat oluşumunu artırma riski nedeniyle bu hastalara yönelik öneri konusu tartışmalıdır. Ancak diyaliz tedavisi alan hastalar değerlendirilirken C vitamininde gözlenen önemli kayıplar ve diyet kısıtlılıkları nedeniyle alımdaki azalmalar dikkate alınmalıdır. Kronik böbrek yetmezliği hastalarında E vitamininin birçok olumlu etkisi bildirilmekle birlikte suplemantasyonun dozu, süresi ve uygulama yolu gibi farklılıklar nedeniyle net öneri vermek mümkün olmamaktadır. Bununla birlikte özellikle E vitamini kaplı diyaliz membran kullanımının oksidatif stresi azaltabileceği düşünülmektedir. Kronik böbrek yetmezliği hastalarında antioksidan vitamin desteğine yönelik çalışmaların devam etmesi gerekmekle birlikte, hastaların diyet antioksidan vitamin alımlarının iyi değerlendirilmesi oldukça önem taşımaktadır.

Antioxidant Vitamins for Cataracts: 15-Year Follow-up of a Randomized Trial

Cataract remains the leading cause of blindness worldwide. 1 Flaxman S.R. Bourne R.R.A. Resnikoff S. et al. Global causes of blindness and distance vision impairment 1990-2020: a systematic review and meta-analysis. Lancet Glob Health. 2017; 5: e1221-e1234 Abstract Full Text Full Text PDF PubMed Scopus (1312) Google Scholar Antioxidant vitamins have been proposed as a potential prophylactic therapy but results from randomized trials have not shown a protective effect thus far. 2 Mathew M.C. Ervin A.M. Tao J. Davis R.M. Antioxidant vitamin supplementation for preventing and slowing the progression of age-related cataract. Cochrane Database Syst Rev. 2012; (CD004567) Crossref Google Scholar Existing trials have largely been conducted in industrialized nations and have had relatively short follow-up. Longer-term studies in developing countries might provide a greater chance of finding an effect because such settings have high rates of both cataract and micronutrient deficiencies. We report 15-year results from such a trial: the Antioxidants for the Prevention of Cataracts (APC) study.

The Effects of a 6-Month High Dose Omega-3 and Omega-6 Polyunsaturated Fatty Acids and Antioxidant Vitamins Supplementation on Cognitive Function and Functional Capacity in Older Adults with Mild Cognitive Impairment.

The aim of the present study was to examine the effects of a high-dose omega-3 and omega-6 fatty acids supplementation, in combination with antioxidant vitamins, on cognitive function and functional capacity of older adults with mild cognitive impairment (MCI), over a 6-month period in a randomized, double-blind, placebo-controlled trial. Forty-six older adults with MCI (age: 78.8 ± 7.3 years) were randomized 1:1 to receive either a 20 mL dose of a formula containing a mixture of omega-3 (810 mg Eicosapentaenoic acid and 4140 mg Docosahexaenoic acid) and omega-6 fatty acids (1800 mg gamma-Linolenic acid and 3150 mg Linoleic acid) (1:1 w/w), with 0.6 mg vitamin A, vitamin E (22 mg) plus pure γ-tocopherol (760 mg), or 20 mL placebo containing olive oil. Participants completed assessments of cognitive function, functional capacity, body composition and various aspects of quality of life at baseline and following three and six months of supplementation. Thirty-six participants completed the study (eighteen from each group). A significant interaction between supplementation and time was found on cognitive function (Addenbrooke’s Cognitive Examination -Revised (ACE-R), Mini-Mental State Examination (MMSE) and Stroop Color and Word Test (STROOP) color test; p < 0.001, p = 0.011 and p = 0.037, respectively), functional capacity (6-min walk test and sit-to-stand-60; p = 0.028 and p = 0.032, respectively), fatigue (p < 0.001), physical health (p = 0.007), and daily sleepiness (p = 0.007)—showing a favorable improvement for the participants receiving the supplement. The results indicate that this nutritional modality could be promising for reducing cognitive and functional decline in the elderly with MCI.

The Effects of Strength Training Combined with Vitamin C and E Supplementation on Skeletal Muscle Mass and Strength: A Systematic Review and Meta-Analysis.

Intense muscle contractile activity can result in reactive oxygen species production in humans. Thus, supplementation of antioxidant vitamins has been used to prevent oxidative stress, enhance performance, and improve muscle mass. In this sense, randomized controlled studies on the effect of vitamin C and E supplementation combined with strength training (ST) on skeletal muscle mass and strength have been conducted. As these studies have come to ambiguous findings, a better understanding of this topic has yet to emerge. The purpose of the present review is to discuss the current knowledge about the effect of vitamin C and E supplementation on muscle mass and strength gains induced by ST. Search for articles was conducted in the following databases: PubMed/Medline, Web of Science, Scopus, Cochrane Central Register of Controlled Trials, and Google Scholar. This work is in line with the recommendations of the PRISMA statement. Eligible studies were placebo-controlled trials with a minimum of four weeks of ST combined with vitamin C and E supplementation. The quality of each included study was evaluated using the Physiotherapy Evidence Database Scale (PEDro). 134 studies were found to be potentially eligible, but only seven were selected to be included in the qualitative synthesis. A meta-analysis of muscle strength was conducted with 3 studies. Findings from these studies indicate that vitamins C and E has no effect on muscle force production after chronic ST. Most of the evidence suggests that this kind of supplementation does not potentiate muscle growth and could possibly attenuate hypertrophy over time.

Recurrent Indoor Environmental Pollution and Its Impact on Health and Oxidative Stress of the Textile Workers in Bangladesh.

Perennial indoor environmental pollution in the textile industrial area is a potential health hazard for workers engaged in this line of work, resulting in mental aberration to severe health risks. This study was designed to investigate the indoor environmental quality of textile industries and correlate its effect on the occupational health and well-being of the textile workers by measuring plasma oxidative stress status in textile workers and healthy control subjects. Environmental samples were collected from 15 textile industries located in Dhaka division, and 30 volunteer textile workers and 30 volunteer office workers (control) aged 18 to 57 years participated in the study. The concentration of plasma ascorbic acid (P-ASC), plasma malondialdehyde (P-MDA), and plasma conjugated diene (P-CD) was measured in both groups. The noise level (78.0 ± 0.68 dB) and the formaldehyde level (141.80 ± 4.47 µg/m3) were found to be significantly higher in the indoor environmental area compared with those in the control area (70.17 ± 0.25 dB and 108.0 ± 0.76 µg/m3, respectively). Furthermore, the daily average concentration of suspended particulate matters (PMs), that is, PM2.5 (322.2 ± 13.46 µg/m3) and PM10 (411.0 ± 17.57 µg/m3), was also found to be significantly higher in the indoor environmental air compared with that in the control area (78.59 ± 1.66 and 174.0 ± 2.33 µg/m3, respectively). The levels of P-MDA (0.37 ± 0.03 nmol/L) and P-CD (14.74 ± 0.61 nmol/L) were significantly increased, whereas the level of P-ASC level (0.46 ± 0.04 mg/dL) was markedly decreased in the textile workers compared with the healthy control subjects (0.18 ± 0.01 nmol/L of P-MDA, 10.04 ± 0.44 nmol/L of P-CD, and 1.29 ± 0.06 mg/dL of P-ASC). The textile plants were found to have significantly elevated levels of indoor environmental pollutants compared with those in the control area, and the textile workers were significantly exposed to oxidative stresses compared with the control subjects. The use of noise pads and high-efficiency air filters is perhaps highly instrumental to put an end to this prevailing situation. Moreover, to overcome the oxidative stresses among workers, supplementation of antioxidant vitamins (ie, ascorbic acid and/or vitamin E) may be beneficial. In addition, to prevent serious health-related issues, proper precautions should be taken to protect the occupational health of the textile workers.

Relevance of antioxidant vitamin supplementation for improvement of milk production, milk quality and energy status of lactating ewes

This study aimed to determine the potential benefits of vitamin A or C supplementation for lactating ewes in terms of milk production, milk quality and energy status. Late-stage pregnant Rahmani ewes received an oral dose of either 50.000 IU of vitamin A (n = 8) or 3 g of vitamin C daily (n = 8) and were compared with ewes that did not receive any vitamin dose (control, n = 7). The milk yield was determined, and milk samples were collected and analysed for milk compositions and fatty acid (FA) profiles. The energy status of the ewes was evaluated by estimating the milk energy value (MEV), net energy required for lactation (NEL) and energy balance calculated from milk components (EBM). The antioxidant activity of the milk was evaluated by determining the 1,1-diphenyl-2-picrylhydrazyl (DPPH)scavenging activity and the concentration of malondialdehyde (MDA, lipid peroxidation index). The quality of milk FAs was determined by estimating the atherogenicity index (AI) and the concentration of hypercholesterolaemic FAs (C12:0 + C14:0 + C16:0). The results revealed that vitamin A treatment resulted in the greatest milk yield (P = 0.073), energy-corrected milk yield (ECM, P ≤ 0.05), fat yield (P = 0.034) and NEL (P = 0.049); however, the MEV and EBM were not affected by the treatment. Both vitamins increased (P < 0.05) the concentrations of C13:0, C17:0, C18:1 and total mono-unsaturated fatty acids (MUFAs). The antioxidant activity of the milk produced by ewes that received either vitamin A or C was significantly greater than that of the milk produced by control ewes, as indicated by the increased DPPH scavenging activity and decreased MDA concentration. The FA quality was improved in both vitamin-treated ewes compared to the control ewes, as both the AI and hypercholesterolaemic FA levels decreased. In summary, administration of vitamin A or C to lactating ewes from late pregnancy to mid-lactation improved milk quality by increasing the concentrations of healthy FAs and the antioxidant capacity of the milk. In the context of animal productivity, vitamin A should be given priority due to the specific potential of this vitamin to improve milk yield and energy utilization compared to vitamin C.

ANTIOXIDANT VITAMINS SUPPLEMENTATION DURING 7-DAYS HIGH-SALT DIET PREVENTS IMPAIRMENT OF MICROVASCULAR RECTIVITY IN RESPONSE TO VASCULAR OCCLUSION IN YOUNG HEALTHY INDIVIDUALS

Objective: Our earlier studies demonstrated that 7-days high-salt (HS) diet impairs vascular reactivity in forearm skin microcirculation, as well as increases level of oxidative stress in young healthy individuals, independently of arterial blood pressure (BP) changes. The aim of the present study was to test whether HS diet-induced increased oxidative stress have direct role in observed microvascular changes by functionally testing if antioxidant vitamins supplementation during 7-days HS diet will prevent impairment of microvascular reactivity in response to vascular occlusion in young healthy individuals. Design and method: 55 young healthy individuals participated in this study (27 men, 28 women, age ranged 18–26). All subjects took 7-days low-salt (LS) diet – washout period (<3.2 g salt/day) followed by 7-days HS diet (∼14 g salt/day) without (HS group) or with vitamin C (1000 mg/day) and E (800 IU/day) supplementation (HS+C+E group). Forearm skin microvascular reactivity in response to vascular occlusion - post-occlusive reactive hyperemia (PORH) was assessed by laser Doppler flowmetry (LDF) before and after diet protocols. BP and heart rate (HR) were measured at the beginning of each study visit. Compliance to the prescribed LS and HS diet was assessed by calculating salt intake from 24 h urinary sodium excretion. Results: There was no change in BP and HR after HS or HS+C+E protocol compared to LS. Changes in 24 h urinary sodium excretion confirmed that subjects conformed to the diet protocols. Consistent with our earlier results, PORH was significantly impaired in HS group (R-O% LS 135 ± 34 vs. HS 105 ± 19, P < 0.001). However, such impairment was not observed, and PORH was not changed in group taking antioxidant vitamins during HS diet (R-O% LS 133 ± 25 vs. HS+C+E 131 ± 27, P = 0.813). Conclusions: The results of the present study confirmed our previous findings that 7-days HS diet affects peripheral microvascular reactivity independently of BP changes, and for the first time demonstrated that antioxidant vitamins (C and E) supplementation during HS diet prevents microvascular function impairment in healthy individuals. These results indicate that increased level of oxidative stress level presents an important mechanism (and link) through which HS adversely affects vascular function in healthy normotensive individuals.

Antioxidant vitamin supplementation prevents oxidative stress but does not enhance performance in young football athletes.

The aim of this study was to verify the effects of supplementation with antioxidants (vitamins C and E) on oxidative stress, delayed-onset muscle soreness (DOMS), and performance in football players during a recovery period after an exercise-induced oxidative stress protocol. Twenty-one football athletes were randomly assigned to two groups: placebo and antioxidant-supplemented. Supplementation was performed in a double-blind, controlled manner using vitamin C (500 mg/d) and E (400 UI/d) for 15 d. After 7 d of supplementation, athletes were submitted to an exercise-induced oxidative stress protocol consisting of plyometric jumping and strength resistance sets to exhaustion. Blood samples, performance tests, and DOMS were determined before and 24, 48, and 72 h after exercise. Antioxidant supplementation was continued during the recuperation week and for a total of 15 d. Antioxidant supplementation caused a significant increase in plasma vitamins C and E. The antioxidant supplementation could inhibit oxidative stress characterized by elevated lipid peroxidation markers malondialdehyde and total lipid peroxidation as well as reduced ratio of glutathione to oxidized glutathione promoted by exercise. Antioxidant supplementation, however, did not significantly reduce the plasma creatine kinesis concentration or DOMS during the recovery days. Likewise, supplementation with vitamin C and E did not improve lower body power, agility, or anaerobic power, nor did it provide any indication of faster muscle recovery. Antioxidant supplementation does not attenuate elevated markers of muscle damage or muscle soreness promoted by acute exercise and do not exert any ergogenic effect on football performance of young athletes, although it reduced oxidative stress.

Effect of Vitamin A on Lung Function Test in patient with Chronic Bronchial Asthma

In chronic bronchial asthma inflammation may be accompanied by intensive air flow limitation. Endogenous oxidants produced by overactive inflammatory cells destroy airway epithelium which slough into bronchial lumen and thus aggravates asthma. When oxidant overwhelm anti-oxidants, tissue injury and disease results. It is observed that decrease level of anti-oxidants in the lungs is a feature of chronic bronchial asthma and that there is a marked decrease of these levels during acute asthmatic attacks. These observations highlight the positive correlation between anti-oxidant therapy in asthmatic patients. The study was carried out to observe the changes in lung function in patients with chronic bronchial asthma both before and after supplementation of anti-oxidant vitamin A. Pulmonary function variables such as FVE, FEV1, FEV1/FVC%and PEFR were measured by spirometer in patients with chronic bronchial asthma both before and three month after supplementation of vitamin A 10,000 I.U orally daily. The mean FVC, FEV1, FEV1/FVC% and PEFR following vitamin A was unchanged significantly (P&lt;0.001) than the pre supplementation values in patients with chronic bronchial asthma. This study reveals that no improvement of pulmonary functions occur after supplementation of anti-oxidant vitamin A in chronic bronchial asthma patients.TAJ 2014; 27(1): 10-13

The use of antioxidant vitamin supplements among oncological patients.

Dietary supplementation is becoming more and more common among both healthy and unhealthy people. The use of supplements is often unjustified, though in some groups of patients it is a necessary management for providing the required vitamins and minerals. The aim of the study was to assess the frequency of using antioxidant vitamin supplements (A, C and E) among the patients of the oncology ward. The study group included 78 patients aged 19-83 years. The dietary intake of vitamins as well as the intake of supplements was assessed based on the data from the Food Frequency Questionnaire (FFQ). It was observed that 46.2% of patients used some kind of a dietary supplement and 77.8% of them used antioxidant vitamins. Among those taking vitamin A, C or E supplements, 72.2% of women and 80% of men used multivitamins. It was reported that the average fulfillment of the recommended daily intake for vitamin A was 303 ±136%, for vitamin C it was 282 ±166% and for vitamin E it was 199 ±80%. More than 25% of the patients whose diets contained at least the same level of vitamins as dietary recommendations were using antioxidant vitamin supplements at the same time. Although the average dietary intake of antioxidant vitamins among the patients was not insufficient, the use of dietary supplements in different forms was common in our study. The results of other studies concerning the safety of using dietary supplements by cancer patients are not conclusive. Dietary supplementation in oncological patients should always be used after a medical consultation with a doctor and a dietician.