الإجهاد التأکسدى ومضادات الأکسدة للفئران المصابة بالسکرى والتى تم تغذیتها على بعض مستخلصات الأجزاء النیاتیة Oxidative stress and antioxidant defense systems status in diabetic rats feeding some selected plant partexctract

Abstract

Abstract:Oxidative stress plays a pathological role in the development of various diseases including diabetes. Systemic oxidative stress results from an imbalance between oxidants derivatives production and antioxidants defenses. The present study aims to investigate the oxidative stress and antioxidant defense system status in diabetic rats feeding some selected plant parts such okra fruits water extract (OFWE), white mulberry leaves water extract (MLWE), celery leaves water extract (CLWE) and their Mixture. Treatment of animals with alloxancaused a significant increased (p≤0.05) in serum glucose concentration by the ratio 136.51% compared to normal animals (negative control group). Supplementation of the rat diets with 2% w/w by OFWE, MLWE, CLWE and their mixture decreased this elevation value compared to normal controls and recorded 57.35, 38.69, 52.27 and 35.48%, respectively. Also, consumption of OFWE, MLWE, CLWE and their mixture induced significant improvements on plasma glutathione fractions concentration through increasing the reduced glutathione (GSH) and oxidized glutathione (GSSG)compared to normal controls by the ratio of -15.31; -11.22, -14.29 and -7.14%; and -15.31, -11.22, -14.29 and -7.14%, respectively. The opposite direction was observed with the biological oxidants includingthiobarbituric acid reactive substances (TBARS) and nitric oxides (NO2 and NO3).In conclusion,the present data support the benefits of dietary modification, including bioactive compounds and antioxidant vitamins supplementation, in alleviating oxidative stress associated diabetes.