Supplementary Carbon Source Research Articles

The effect of carbon source supplementation on the production of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) by Cupriavidus necator

The objective of the present study was to investigate the ability of Cupriavidus necator to produce poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) on various carbon sources in batch cultivation. These results show that C. necator produces poly-3-hydroxybutyrate from single carbon sources. The highest poly-3-hydroxybutyrate (P3HB) content was achieved at growth on fructose in the exponential growth phase. The maximum yield of the P3HV content was obtained when fructose was mixed with acetate. The highest content P3HB-co-3HV was also achieved by C. necator when we supplied C-excess and N- and P-normal conditions. These results indicate that C. necator accumulates high polyhydroxyalkanoates (PHA) content by depleting these elements in the culture medium. Nitrogen and phosphorus limitation has no significant effect on the PHA production, whereas C-excess leads to an increase in PHA formation of up to 92% PHAs of cell dry weight after growth on 5 g/L acetate and 40 g/L fructose.

유류누출 지역에서 유래한 토착세균, Pseudoalteromonas sp. HK-3 배양에서 생물계면활성제의 최적 생산

The principal objective of this study was to determine the optimal conditions for the production of biosurfactant by the indigenous bacterium, Pseudoalteromonas sp. HK-3, originating from oil-spilled areas. The relationship between total biosurfactant production and the factors affecting biosurfactant production were evaluated by statistical analysis using SPSS software. The effects of various supplemental carbon sources (e.g., glucose, dextrose, mannitol, citrate, acetate) on the maximal production of biosurfactant by the test culture of Pseudoalteromonas sp. HK-3 was then evaluated. As a result, mannitol was found in this study to be the best supplemental carbon source for the production of biosurfactant. A spot inoculation of crude cultural liquid containing the HK-3 cells generated the largest clear zone, whereas only small clear zones appeared around the spots inoculated with either supernatant only or cell pellets following centrifugation. Our results demonstrated that the HK-3 test culture supplemented with 2% mannitol at an initial pH of 6 generated the maximal amount of biosurfactant within 72 h of incubation.

Study on nitrogen removal enhanced by shunt distributing wastewater in a constructed subsurface infiltration system under intermittent operation mode

Subsurface wastewater infiltration system is an efficient and economic technology in treating small scattered sewage. The removal rates are generally satisfactory in terms of COD, BOD 5, TP and SS removal; while nitrogen removal is deficient in most of the present operating SWIS due to the different requirements for the presence of oxygen for nitrification and denitrification processes. To study the enhanced nitrogen removal technologies, two pilot subsurface wastewater infiltration systems were constructed in a village in Shenyang, China. The filled matrix was a mixture of 5% activated sludge, 65% brown soil and 30% coal slag in volume ratio for both systems. Intermittent operation mode was applied in to supply sufficient oxygen to accomplish the nitrification; meanwhile sewage was supplemented as the carbon source to the lower part in to denitrify. The constructed subsurface wastewater infiltration systems worked successfully under wetting-drying ratio of 1:1 with hydraulic loading of 0.081 m 3/(m 2 d) for over 4 months. Carbon source was supplemented with shunt ratio of 1:1 and shunt position at the depth of 0.5 m. The experimental results showed that intermittent operation mode and carbon source supplementation could significantly enhance the nitrogen removal efficiency with little influence on COD and TP removal. The average removal efficiencies for NH 3-N and TN were 87.7 ± 1.4 and 70.1 ± 1.0%, increased by 12.5 ± 1.0 and 8.6 ± 0.7%, respectively.

Studies on cytochromes of lichenized fungi under optimized culture conditions

Cytochromes are membrane-bound hemoproteins responsible for the generation of ATP via the electron transport system to fuel the metabolic processes of the organism for their growth. This study reports the properties of cytochromes present in the isolated lichenized fungi of the cultured lichen Usnea ghattensis under optimized conditions. The fungal partner of the cultured lichen Usnea ghattensis contains a, b and c types of cytochromes. The concentrations of a, b and c type cytochromes were found to be significantly high (0.0967, 0.0900, and 0.1030 mM/ mg protein, respectively) in the isolated fungal symbiont of cultured lichen grown in malt-yeast extract medium supplemented with 0.01 mol/l sucrose and 0.01 mol/l polyethylglycol. The results suggest that supplementation of additional carbon sources may play a role in optimizing the growth via activating the cytochrome respiratory system in lichenized fungi.

Studies on cytochromes of lichenized fungi under optimized culture conditions

Cytochromes are membrane-bound hemoproteins responsible for the generation of ATP via the electron transport system to fuel the metabolic processes of the organism for their growth. This study reports the properties of cytochromes present in the isolated lichenized fungi of the cultured lichen Usnea ghattensis under optimized conditions. The fungal partner of the cultured lichen Usnea ghattensis contains a, b and c types of cytochromes. The concentrations of a, b and c type cytochromes were found to be significantly high (0.0967, 0.0900, and 0.1030mM/ mg protein, respectively) in the isolated fungal symbiont of cultured lichen grown in malt-yeast extract medium supplemented with 0.01mol/l sucrose and 0.01mol/l polyethylglycol. The results suggest that supplementation of additional carbon sources may play a role in optimizing the growth via activating the cytochrome respiratory system in lichenized fungi.

Combined electrochemical and biological treatment for pesticide degradation – Application to phosmet

The aim of this study was to determine the feasibility of coupling an electrochemical process with a biological treatment in order to degrade phosmet, an organophosphorous pesticide. The absence of biodegradability of phosmet by Pseudomonas fluorescens and activated sludge was verified in our operational conditions. So, a conventional biological treatment is not appropriate for phosmet polluted effluents. Electrochemical behavior of phosmet was studied by cyclic voltammetry and the feasibility of an electrochemical pretreatment was thus demonstrated. Preliminary results with activated sludge showed a diminution of 26% for COD (chemical oxygen demand) measured when the electrolyzed solution was used as the sole carbon and nitrogen sources. When glucose and ammonium were added as supplementary carbon and nitrogen sources, the COD diminution reached 34% after 79 h of culture. This study demonstrates the feasibility of an electrochemical pre-treatment prior to biotreatment.

Anaerobic Acidogenesis of Food Waste and Application as a Supplemental Carbon Source for Denitrification

Anaerobic Acidogenesis of Food Waste and Application as a Supplemental Carbon Source for Denitrification

The Bethune Point WWTF Utilizes a Non–Flammable Supplemental Carbon Source and Online Nitrate Analyzers for Process Control to Optimize Low Level Nitrogen Removal

The Bethune Point WWTF Utilizes a Non–Flammable Supplemental Carbon Source and Online Nitrate Analyzers for Process Control to Optimize Low Level Nitrogen Removal

Ability of Fungal Strains to Degrade PVA Based Materials

The aim of this work was to select a fungal strain with degradative potential upon PVA based materials. The polymeric materials tested were PVA films which contain different percentages of PVA, starch and glycerol. These materials are of interest for food packaging applications, which presume to solve the problems concerning accumulation, disposal and degradation. Eleven strains were tested in solid culture for the ability to use PVA based composites as carbon and energy sources. The fungal strain selected was cultivated in liquid medium with different compositions. The scanning electron microscopy (SEM) investigations revealed the effects of microorganism growth upon polymeric films. Significant changes in polymer surface aspects were observed depending on the medium culture composition, the presence of supplementary carbon source facilitating microbial growth and degradation process.

Microbial degradation of 17β -estradiol and 17α -ethinylestradiol followed by a validated HPLC-DAD method

This work aimed at studying the biodegradation of two estrogens, 17α -estradiol (E2) and 17β -ethinylestradiol (EE2), and their potential metabolism to estrone (E1) by microbial consortia. The biodegradation studies were followed by High Performance Liquid Chromatography–Diode Array Detector (HPLC–DAD) using a specifically developed and validated method. Biodegradation studies of the estrogens (E2 and EE2) were carried out with activated sludge (consortium A, CA) obtained from a Wastewater Treatment Plant (WWTP) and with a microbial consortium able to degrade recalcitrant compounds, namely fluorobenzene (consortium B, CB). E2 was more extensively degraded than EE2 by CA whereas CB was only able to degrade E2. The addition of acetate as a supplementary carbon source led to a faster biodegradation of E2 and EE2. E1 was detected as a metabolite only during the degradation of E2. The 16S rRNA gene sequence analyses of strains recovered from the degrading cultures revealed the presence of the genera Pseudomonas, Chryseobacterium and Alcaligenes. The genera Pseudomonas and Chryseobacterium were retrieved from cultures supplied with E2 and EE2, while the genus Alcaligenes was found in the presence of E2, suggesting that they might be involved in the degradation of these compounds.

Using Whey as a Supplemental Carbon Source under Real Time Control Conditions …or a story of… Turds and Whey

Using Whey as a Supplemental Carbon Source under Real Time Control Conditions …or a story of… Turds and Whey

Production and regulation of extracellular chitinase from the entomopathogenic fungus Isaria fumosorosea

Extracellular chitinase production by the entomopathogenic fungus, Isaria fumosorosea IF28.2 was studied by using submerged fermentation. Maximum chitinase production (178.34±3.91 mU/mL) was obtained when fermentation was carried out at 25°C for 120 h using 72-h-old mycelium in a medium. The effect of inoculum size on chitinase activity was also observed and maximum chitinase activity (159.41±2.91 mU/mL) was obtained with an inoculum size of 3 discs while an incubation period of 96 h proved the most active inducer of chitinase production yielding a chitinase activity of 186.14±3.81 mU/mL. Colloidal chitin (1.5%, w/v) proved to be the best concentration. The optimum pH for chitinase production was 5.7 while 25°C proved to be the best temperature for chitinase production. Supplementation of additional carbon source like 1.5% N-acetylglucosamine (GlcNAc) showed further enhancement in chitinase production. The divalent metal salts, CaCl2, MgCl2 and ZnSO4, inhibited chitinase activity at 10 and 100 mM concentration, whereas inhibition of chitinase activity by KCl, FeSO4 and EDTA was observed only at higher concentrations. The results presented in this study increase the knowledge on chitinase production in I. fumosoroseus opening new avenues for the study of the role of this enzyme in virulence against different insect pests during the infection process.

Considerations for Alternative Supplemental Carbon Sources in Deep Bed Denitrification Filters to Achieve Low Effluent Nutrient Concentrations

Considerations for Alternative Supplemental Carbon Sources in Deep Bed Denitrification Filters to Achieve Low Effluent Nutrient Concentrations

Biosurfactant production by Pseudomonas aeruginosa SP4 using sequencing batch reactors: Effect of oil-to-glucose ratio

Sequencing batch reactors were used for biosurfactant production from Pseudomonas aeruginosa SP4. The lab-scale aerobic sequencing batch reactor units were operated at an oil loading rate of 2 kg/m 3 day, a cycle time of 2 days/cycle, and a temperature of 37 °C. A mineral medium with palm oil was used as the culture medium. Glucose, a supplemental carbon source, was added for enhancing the microbial growth, which, in turn, gave a better process stability. The optimum oil-to-glucose ratio for the biosurfactant production was 40:1, providing a surface tension reduction of 58.5%, a chemical oxygen demand removal of 85.1%, and an oil removal of 77.7%. The maximum biosurfactant concentration in the bioreactors was about 1.1 times the critical micelle concentration. The biosurfactant product was predominantly composed of Rha-Rha-C 8-C 10 and Rha-Rha-C 10-C 8, and its critical micelle concentration was 150 mg/l.

Optimization of Xylanase Production byThermomyces lanuginosusin Solid State Fermentation

Extracellular xylanase production by the thermophilic fungus Thermomyces lanuginosus 195 in solid state fermentation (SSF) was found to be significantly affected by fermentation temperature, duration, and inoculum volume (p < or = 0.001). Optimization of these parameters corresponded to a 21.7% increase in xylanase yield. Maximum activity (2,335 U/g of wheat bran) was obtained when 10 g of wheat bran was inoculated with 10 ml of liquid culture and cultivated at 45 degrees C for 40 h. The influence of supplemental carbon and nitrogen sources (3% w/v) on xylanase production was also assessed. Wheat bran, supplemented with glucose and cellulose, facilitated 10% and 7% increases in relative activity respectively. Ammonium based salts, nitrates, and a number of organic nitrogen sources served only to reduce xylanase production (p < or = 0.005) significantly. The enhanced xylanase titers achieved in the present study emphasize the need for optimizing growth conditions for maximum enzyme production in SSF.

Nutrient removal in an electrically enhanced membrane bioreactor

Integration of the membrane bioreactor (MBR) into wastewater treatment facilities has gained popularity in recent years due to increasingly stringent discharge permits. However, up to now no research has been conducted on the combination of nitrification, denitrification and electrochemical phosphorus removal into a MBR system. In this study a novel electrically enhanced MBR (EMBR) system was used. Without pH adjustment and external carbon source supplementation, using synthetic feed, ammonium-nitrogen was completely eliminated; COD, total nitrogen and ortho-phosphorus were removed by 94.3%, 77% and 86.6%, respectively. The power consumption was 0.22 kW/m(3) of the influent synthetic wastewater. With a control MBR run in parallel, the applied voltage gradient of 1.82 V/cm did not exhibit adverse influence on the microbial growth. This system has the potential to achieve phosphorus removal through alternating the direct current intensity.

Swine Wastewater Treatment Using Submerged Biofilm SBR Process: Enhancement of Performance by Internal Circulation through Sand Filter

Pollutants removal from swine wastewater by a submerged biofilm sequencing batch reactor (BSBR) with internal circulation of liquor through a sand filter was studied. The variation of nutrient removal efficiencies with changes in volumetric circulation ratios and rates were determined. The reactor was operated under the following conditions: One cycle per day, hydraulic retention time of 15 days, average NH4 –N loading rate of 55 g m−3 d−1 , and without supplemental external carbon source. System performance was enhanced by conducting internal circulation of liquor through the sand filter. When compared with the performance of a single BSBR without sand filter, nitrogen and phosphorus removal efficiencies were found to increase by 18% and over 33%, respectively. With a circulation rate of 170 L h−1 m−3 , and duration of 22 h (circulation ratio of 0.9), TOC, NH4 –N , and total soluble inorganic nitrogen (as NH4 –N plus NOx –N ) removal efficiencies of 73, 97.8, and 85.6%, respectively, were achieved....

The impact of supplemental carbon sources on Arabidopsis thaliana growth, chlorophyll content and anthocyanin accumulation

This research explores the impacts of a broad range of supplemental carbon sources on growth and development of Arabidopsis thaliana. Parameters measured include dark-germinated hypocotyl length, light-germinated root growth, rosette growth, chlorophyll concentration and anthocyanin content. Treatment sugars include sucrose, maltose, d-glucose, d-fructose, l-arabinose, l-fucose, d-galactose, d-mannose, l-rhamnose and d-xylose each supplied at 4, 20 or 100 mM. This comparison of the effect of different carbon sources on multiple parameters and under identical conditions showed that every carbon source had unique qualitative and quantitative effects on Arabidopsis growth and development. Root growth was particularly sensitive to supplemental carbon source. Growth on 100 mM sucrose, maltose, glucose or xylose stimulated root growth by ~100%. Growth on arabinose, fucose, galactose, mannose or rhamnose inhibited root growth by 50% or more. Several sugars that strongly inhibited root growth had either no effect (galactose and fucose) or a positive effect (arabinose) on hypocotyl elongation and rosette growth. Rhamnose was the only carbon source that inhibited hypocotyl elongation across all concentrations. Sucrose, maltose, glucose, fructose, arabinose or xylose stimulated rosette growth by ~50%. Chlorophyll content was strongly reduced by mannose while sucrose, glucose, galactose and rhamnose caused smaller reductions. Anthocyanin accumulation was strongly induced by both galactose and mannose. Only mannose impacted all parameters across all concentrations. Based on these data it can be concluded that the effect of each carbon source on Arabidopsis growth and development is specific in terms of both magnitude and the parameters impacted.

Enhanced biological denitrification of high concentration of nitrite with supplementary carbon source

Nitrite accumulates during biological denitrification processes when carbon sources are insufficient. Acetate, methanol, and ethanol were investigated as supplementary carbon sources in the nitrite denitrification process using biogranules. Without supplementary external electron donors (control), the biogranules degraded 200 mg l(-1) nitrite at a rate of 0.27 mg NO(2)-N g(-1) VSS h(-1). Notably, 1,500 mg l(-1) acetate and 700 mg l(-1) methanol or ethanol enhanced denitrification rates for 200 mg l(-1) nitrite at 2.07, 1.20, and 1.60 mg NO(2)-N g(-1) VSS h(-1), respectively; these rates were significantly higher than that of the control. The sodium dodecyl sulfate polyacrylamide gel electrophoresis of the nitrite reductase (NiR) enzyme identified three prominent bands with molecular weights of 37-41 kDa. A linear correlation existed between incremental denitrification rates and incremental activity of the NiR enzyme. The NiR enzyme activity was enhanced by the supplementary carbon sources, thereby increasing the nitrite denitrification rate. The capacity of supplementary carbon source on enhancing NiR enzyme activity follows: methanol > acetate > ethanol on molar basis or acetate > ethanol > methanol on an added weight basis.

Transparent, highly conductive graphene electrodes from acetylene-assisted thermolysis ofgraphite oxide sheets and nanographene molecules

Transparent and highly conductive graphene electrodes have been fabricated throughacetylene-assisted thermolysis of graphite oxide (GO) sheets. This novel procedure usesacetylene as a supplemental carbon source to repair substantial defects within GO sheets,leading to the enhancement of graphitization of synthesized graphene electrodes. Theas-prepared graphene on quartz substrates exhibits an electrical conductivity of1425 S cm−1 with an optical transmittance of more than 70% at a wavelength of 500 nm. Such anacetylene-assisted thermal treatment approach is also adopted to fabricate grapheneelectrodes from synthetic nanographene molecules, with an almost five times increase inconductivity compared to samples prepared by the common thermal reduction.