Supplementary Treatment Research Articles

Preventive interventional strategies mitigate age-associated degeneration of dorsal hippocampal neural cells in naturally ageing mice

Objectives: The objective of this study was to investigate the effectiveness and potential protective effects of various intervention strategies, such as choline and docosahexaenoic acid (Ch-DHA) supplementation, Clitoria ternatea root extract (CTR) supplements, human embryonic kidney stem cell conditioned media (HEK-CM) treatment and exposure to enriched environments (EEs), in mitigating neural cell degeneration in dorsal hippocampal subregions of naturally ageing mice brain. Materials and Methods: Twelve–fifteen-month-old CF1 male mice were subdivided (n = 6/group) into normal age-matched control, Ch-DHA supplemented, EE exposed, HEK-CM administered and CTR-supplemented groups. Neuro morphological alterations in the dorsal hippocampus were assessed using cresyl violet staining. Results: Higher neural cell degeneration was observed in the CA1–CA3 dorsal hippocampal subregions in normal ageing mice brains. Notably, interventions such as HEK-CM administration, Ch-DHA supplementation, exposure to an EE and CTR supplementation significantly reduced degeneration, particularly in the CA3 and CA2 regions. Conclusion: Ch-DHA supplementation and HEK-CM treatment are observed to significantly reduce age-dependent degeneration of dorsal hippocampal CA3 and CA2 neurons in naturally ageing mice compared to EE exposure or CTR supplementation.

Efficacy of microwave ablation in the treatment of large benign thyroid nodules: a multi-center study.

To evaluate the efficacy, safety, and improvement of symptoms by ultrasound-guided microwave ablation (MWA) for patients with large benign thyroid nodules (BTNs). Eighty-seven patients with 87 BTNs (≥ 4cm) treated with MWA between April 2015 and March 2021 were enrolled in this retrospective multicenter study, with clinical and ultrasound examinations performed at the 1st, 3rd, 6th, and 12th months. A multivariable linear mixed effects model was employed to explore the alterations in volume and volume reduction ratio (VRR), as well as the potential factors associated with VRR. The mean age of the 87 patients was 45.69 ± 14.21years (range 18-76years), and the ratio of men to women was 1:4.8. The mean volumes were much decreased at the 12th month after ablation compared to the initial volumes (p < .001). The mean VRR was 76.09% at the 12th month. The technique efficacy (VRR > 50%) was 90.80% at the 12th month. A multivariate analysis revealed that VRR was related to the initial volume (p = .015), annular flow (p = .010), and nodule composition (p = .024). The mean symptomatic score decreased from 4.40 ± 0.28 to 0.26 ± 0.06 at the 12th month (p < .001). At the same time, the mean cosmetic score decreased from 3.22 ± 0.10 to 1.31 ± 0.08 (p < .001). MWA could serve as a safe and effective therapy for large BTNs, significantly reducing the volume of BTNs and significantly improving compressive symptoms and appearance problems. Microwave ablation could serve as a safe and effective therapy for large benign thyroid nodules, leading to significant volume reduction and satisfied symptom and cosmetic alleviation period. • This multicenter study investigated the feasibility and safety of microwave ablation for large benign thyroid nodules. • After ablation, the nodule volume was significantly reduced, and patients' symptoms and appearance problems were significantly improved. • Microwave ablation is feasible for large benign thyroid nodules and has been a supplement treatment.

Homeopathic Pyrogenium Ointment as Adjuvant in Treatment of Traumatic and Infected Myiasis by Cochliomyia Hominivorax (Diptera: Calliphoridae) in Sheep: A Case Series.

The blowfly Cochliomyia hominivorax (Diptera: Calliphoridae) causes traumatic myiasis in farm animals, with deeply embedded larvae that lead to serious tissue damage and sometimes death. Treating this form of myiasis is difficult, often resulting in poor outcomes. As a homeopathic preparation that can mimic traumatic myiasis symptoms, Pyrogenium could potentially supplement treatment. This study aimed to describe the adjuvant effect that a Pyrogenium ointment might have in the treatment of sheep with traumatic myiasis. The study reports a homeopathic adjuvant treatment with Pyrogenium 12cH ointment in six sheep with traumatic and infected myiasis. Daily clinical examinations were performed to monitor the evolution of the health of animals. Pyrogenium 12cH ointment was applied topically on the wounds, after cleaning and mechanical removal of the larvae from the lesion. Blood samples were collected for blood counts, and wound exudate samples were obtained for bacteriological examination before and after wound treatment. The ointment of Pyrogenium 12cH limited the development of parasitic larvae and was associated with the repair of tissue losses resulting from the myiases and with subsequent wound healing, together with control of fever and local inflammation. Homeopathic Pyrogenium 12cH, formulated as an ointment, may be a useful new and adjuvant therapeutic option for treating traumatic and infected myiasis in animals.

Neurocognitive effects a combined polyphenolic-rich herbal extract in healthy middle-aged adults – a randomised, double-blind, placebo-controlled study

ABSTRACT Objective: This study assessed whether polyphenolic rich supplement containing Bacopa monnieri (BM: 300 mg), Panax quinquefolius ginseng (PQ: 100 mg) and whole coffee fruit extract (WCFE: 100 mg) could enhance cognitive performance, affect and cerebral-cortical activation over 28-days of intervention. Method: A randomised, double-blind, placebo-controlled, between-group study of 52 healthy adults between 35 and 65 years (M = 50.20, SD = 9.37) was conducted. Measures of cognition, affect and brain activity were measured at three time points: baseline, 28 days post intervention and 14 days post washout. At each time point, haemodynamic response in the prefrontal cortex (PFC) was measured using functional near-infrared spectroscopy (fNIRS), and serum brain-derived neurotrophic factor (BDNF). Results: The polyphenolic-rich supplement reliably improved positive affect and delayed recall compared to placebo following 28 days of supplementation. For the brain, those in the active condition showed greater PFC activation on performance of the 2-back tasks post supplementation compared to placebo (p < .05, d = 0.6). Discussion: This is the first report of a 28-day supplement intervention and 2-week follow-up study to assess changes in affect, cognition, cerebral haemodynamic response and BDNF in healthy middle-aged adults. The potential synergistic effects of polyphenolic compounds on neurocognitive function in middle-aged adults through emotional-cognitive processing and cognitive reserve are important for promoting brain and cognitive health.

The advancement in irrigation solution within the field of endodontics, A Review

The purpose of endodontic treatment is to eliminate all germs, microbial by-products, and vital tissues from the root canal system. Root canals can be mechanically and chemically used for this purpose. The needs and details of the irrigation solutions are described in this article. Because of its wide range of antibacterial activities and its ability to dissolve organic tissues, sodium hypochlorite is suggested as the main irrigation. On the contrary, chelation solutions are recommended as supplemental treatments to either eliminate the smear layer or prevent it from forming on the dentin surface. Data: Only articles that were published electronically were searched within the review. Sources: ‘Google Scholar’ and "Pub-med" websites were used to search data, The most papers related to the topic were chosen, specifically original articles , including only articles from 1999 to 2023.Journal papers that weren't published in a highly regarded publication, irrelevant studies, editorials, personal viewpoints, and social media sources were all disqualified from consideration. 98 articles were retained after the filtering procedure. In conclusion, this article reviews many types of new irrigations that may be used in endodontic practice in the future.

Effectiveness of Postnatal Maternal or Caregiver Interventions on Outcomes among Infants under Six Months with Growth Faltering: A Systematic Review.

The care of infants at risk of poor growth and development is a global priority. To inform new WHO guidelines update on prevention and management of growth faltering among infants under six months, we examined the effectiveness of postnatal maternal or caregiver interventions on outcomes among infants between 0 and 6 months. We searched nine electronic databases from January 2000 to August 2021, included interventional studies, evaluated the quality of evidence for seven outcome domains (anthropometric recovery, child development, anthropometric outcomes, mortality, readmission, relapse, and non-response) and followed the GRADE approach for certainty of evidence. We identified thirteen studies with preterm and/or low birth weight infants assessing effects of breastfeeding counselling or education (n = 8), maternal nutrition supplementation (n = 2), mental health (n = 1), relaxation therapy (n = 1), and cash transfer (n = 1) interventions. The evidence from these studies had serious indirectness and high risk of bias. Evidence suggests breastfeeding counselling or education compared to standard care may increase infant weight at one month, weight at two months and length at one month; however, the evidence is very uncertain (very low quality). Maternal nutrition supplementation compared to standard care may not increase infant weight at 36 weeks postmenstrual age and may not reduce infant mortality by 36 weeks post-menstrual age (low quality). Evidence on the effectiveness of postnatal maternal or caregiver interventions on outcomes among infants under six months with growth faltering is limited and of 'low' to 'very low' quality. This emphasizes the urgent need for future research. The protocol was registered with PROSPERO (CRD42022309001).

Wirksamkeit manueller thorakaler Therapie zur Behandlung des drohenden Impingement-Syndroms: systematische Übersicht

Abstract Background Impingement syndrome, a common cause of shoulder pain, often leads to functional limitations. Manual thoracic therapy is frequently employed as a non-surgical intervention, but its efficacy remains debated. This review assesses the impact of manual thoracic therapy on pain reduction and functional improvement in impingement syndrome. Methods A systematic review of randomized clinical trials was conducted, focusing on studies that applied manual thoracic therapy to patients with impingement syndrome. The primary outcomes were pain reduction and functional improvement. Studies were evaluated for methodological quality using the PEDro scale, with scores ≥ 6 indicating high quality. Results Nine studies met the inclusion criteria. All studies demonstrated high methodological quality (PEDro score ≥ 6). Pain reduction was consistent across studies, with an NPRS score reduction of 0.6 to 1.5 points immediately after treatment and up to 3.2 points at follow-up. Functionality improvements were statistically significant in some studies. However, the results showed limited homogeneity, and the majority of studies did not report substantial differences between intervention and placebo groups. Conclusion This review suggests that manual thoracic therapy may lead to pain reduction in impingement syndrome, with some evidence of functional improvement. However, the variability in manual therapy techniques and the limitations in research methodologies indicate a need for further controlled studies. These findings underscore the potential of manual therapy as a supplementary treatment but also highlight the necessity for more robust clinical trials to fully ascertain its effectiveness in clinical practice. Graphic abstract

Feline exocrine pancreatic insufficiency: case report

Feline Exocrine Pancreatic Insufficiency is a disease where the digestion and absorption of nutrients its impaired due to lacking pancreatic enzymes in the intestine. This study aimed to report a case of EPI in a femeale cat, with five years of age, that presented weight loss, liquid diarrhea, and coat alterations. A hemogram and serum biochemestry (ALT and Creatinine), coproparasitological and abdominal ultrasonography were requested. Due to clinical worsening, test results, hepatomegaly and splenomegaly in the imaging exam, serum mensuration for Free T4, Cobalamin, Folate, Albumin, Globulin, serum GGT, Ionic Calcium, SOdium, Potassium, Total Bilirrubin, Indirect Bilirrubin, Direct Bilirrubin, Urinalyses, urinary creatinin-protein ratio, and especific pancratic lipase were requested, and results did not displayed significative alterations. Hospitalization and fluidotherapy were indicated, along with fenbendazol, probiotic, and cobalamin supplementation treatment, as well as hypoalergeninc diet. After, supplementation with pancreatic enzymes begun in order to verify EPI, resulting in clinical manifestations improvement, and feline trypsinogen was asssesed at 7mcg/L, indicating the correct diagnosis assumption.

Therapeutic efficacy and anti-inflammatory mechanism of baicalein on endometriosis progression in patient-derived cell line and mouse model

BackgroundBaicalein is a flavonoid extracted from the roots of Scutellaria baicalensis G. that has anti-inflammatory and antitumor effects. However, therapeutic mechanisms of baicalein in patients with endometriosis in vivo have yet to be elucidated. As a chronic inflammatory gynecological disease, endometriosis causes pain and infertility, and has no complete treatment to date. Current treatment strategies cause several side effects and have high recurrence rates. PurposeThis study aimed to identify the in vivo therapeutic effects of baicalein on endometriosis and verify the action mechanisms of baicalein, focusing on regulating inflammation. MethodsIn this study, an autologous transplant mouse model and patient-derived immortalized human ovarian endometriotic stromal cells (ihOESCs) were used to investigate the therapeutic activities of baicalein. The mouse model was administered with 40 mg/kg baicalein by oral gavage for 4 weeks, and the treatment outcomes of baicalein-treated mice were compared with vehicle- and dienogest-treated groups. ihOESCs were treated with 0–5 μg/ml baicalein for in vitro studies. ResultsBaicalein significantly alleviated the progression of endometriosis in mouse models. Baicalein reduced the expression of proinflammatory cytokines in endometriotic lesions and ihOESCs, and cytokine expression and T cell proportions in mouse spleen. in vitro results showed that baicalein increased mitochondrial calcium flux and induced mitochondrial depolarization and ROS generation in ihOESCs. Ultimately, baicalein inactivated the MAPK/PI3K signaling and induced cell death in ihOESCs. ConclusionIn conclusion, baicalein effectively attenuated the progression of endometriosis through its anti-inflammatory activities. Baicalein can be an alternative or supplemental treatment for endometriosis to ameliorate the side effects of hormonal therapy.

Analysis of Osteosarcoma Cell Lines and Patient Tissue Using a 3D In Vivo Tumor Model—Possible Effects of Punicalagin

Osteosarcomas are the most common primary malignant bone tumors and mostly affect children, adolescents, and young adults. Despite current treatment options such as surgery and polychemotherapy, the survival of patients with metastatic disease remains poor. In recent studies, punicalagin has reduced the cell viability, angiogenesis, and invasion in cell culture trials. The aim of this study was to examine the effects of punicalagin on osteosarcomas in a 3D in vivo tumor model. Human osteosarcoma biopsies and SaOs-2 and MG-63 cells, were grown in a 3D in vivo chorioallantoic membrane (CAM) model. After a cultivation period of up to 72 h, the tumors received daily treatment with punicalagin for 4 days. Weight measurements of the CAM tumors were performed, and laser speckle contrast imaging (LSCI) and a deep learning-based image analysis software (CAM Assay Application v.3.1.0) were used to measure angiogenesis. HE, Ki-67, and Caspase-3 staining was performed after explantation. The osteosarcoma cell lines SaOs-2 and MG-63 and osteosarcoma patient tissue displayed satisfactory growth patterns on the CAM. Treatment with punicalagin decreased tumor weight, proliferation, and tumor-induced angiogenesis, and the tumor tissue showed pro-apoptotic characteristics. These results provide a robust foundation for the implementation of further studies and show that punicalagin offers a promising supplementary treatment option for osteosarcoma patients. The 3D in vivo tumor model represents a beneficial model for the testing of anti-cancer therapies.

165 Does supplementation of beef cattle impact the agronomy performance of tall fescue and bermudagrass pastures?

Abstract In forage-based beef cattle systems, bermudagrass [Cynodon dactylon (L.) Pers.] and tall fescue (Festuca arundinacea Schreb.) stand as widely used forage species. The strategic supplementation of animals may reduce forage intake, thereby potentially affecting the agronomic performance of these grasses. Our objective was to evaluate the impact of beef cattle supplemental feeding on forage accumulation, forage mass, and nutritive value in tall fescue and bermudagrass pastures. Two studies were conducted where pregnant beef cows were supplemental fed 1% of BW on DM basis of corn silage daily (SUP) or received no supplemental feeding (free-choice mineral; NON). Trial 1: conducted on bermudagrass pastures (BERM) grazed from late May to early October. Experimental design for BERM was completely randomized design with 4 pastures per treatment (n = 3 cows/pasture; average 5 years of age; 550 ± 72 kg initial BW). Trial 2: conducted on fescue pastures (FES) with an experimental design of a 2 x 2 factorial arrangement. The two factors evaluated were fescue type [TOXIC (507 ppm ± 0.82 SEM ergot alkaloids; 92.5% endophyte infection)] or non-toxin-producing (NT; 5 ppm ± 2.50 SEM ergot alkaloids; 38.6% endophyte infection) and two levels of supplemental feeding (NON or SUP). There were 3 or 4 replications per fescue x supplemental feeding treatment (n = 15 pastures), stocked with 3 cows/pasture (average age 4.75 years; 558 ± 73 kg initial BW) and grazed from early May through November. Forage accumulation (FA) was estimated every 28 d using the paired-cage method (4 exclosure cages/pasture). On each exclusion period, the forage mass (FM) was estimated both inside and outside the cages through destructive sampling method and samples analyzed for forage quality. Pasture was experimental unit and repeated measures were included in analysis. Bermudagrass FM tended to be increased by 31% with SUP (P = 0.09; 774 kg/ha greater). Forage accumulation tended (P = 0.07) to be affected by feeding method x month. There was greater FA in June for SUP; however, there was greater FA for NON in July. SUP pastures had greater ADF (1% more; P = 0.01) and reduced TDN (1.5%; P &amp;lt; 0.01) than NON. Forage mass tended to be 10% greater for TOXIC pastures (P = 0.09; 409 kg/ha more forage mass). Supplemental feeding on fescue did not impact FM (P = 0.74). Forage accumulation was not impacted by fescue type, feeding method, nor the interaction (P &amp;gt; 0.30). Cows generally do not consume as much toxic fescue, thus more forage is available in pastures and supplemental feeding did not alter forage parameters. However, on a warm-season grass that is growing while supplemental feeding, forage availability was increased and maybe useful as method to increase stocking rates or as mitigation tool during drought.

Sulfur Supplementation Enhanced the Growth and Photosynthesis of Lettuce in Hydroponic Production Using One-bag Complete Fertilizer

Sulfur (S) is an essential plant nutrient that regulates plant growth and metabolism. However, S is often absent from certain one-bag hydroponic fertilizers designed to provide a complete and balanced mixture of nutrients. We quantified the effects of S supplementation on the growth, morphology, and photosynthesis of lettuce grown in a deep-water culture hydroponic system. Two lettuce (Lactuca sativa) cultivars, green butterhead Rex and red oakleaf Rouxai, were grown using a prepackaged fertilizer specially formulated for reverse osmosis (RO) and other low-alkalinity water sources. The base nutrient solution was mixed using Jack’s FeED 12–4–16 fertilizer and RO water at a nitrogen concentration of 100 mg⋅L−1 (control). Three S supplementation treatments were implemented over a 4-week production period: 10 mg⋅L−1 supplemental S (provided using MgSO4); 20 mg⋅L−1 supplemental S (MgSO4); and a treatment using H2SO4 (instead of nitric acid) for pH adjustment. In both lettuce cultivars, shoot fresh and dry mass, total leaf area, leaf photosynthetic rate, total chlorophyll content, and leaf S concentration with all three S supplementation treatments increased significantly compared with those of the control. In contrast, the ratio of shoot dry mass to fresh mass, root dry mass, and percentage of root dry mass (i.e., root dry mass/total shoot and root mass) were significantly higher with the control treatment. Notably, ‘Rouxai’ lettuce grown in the control treatment had intense red coloration with a 216.6% to 288.9% increase in the anthocyanin index. There were no statistical differences in any of the growth and morphological parameters among the three S supplementation treatments. Overall, we observed significantly enhanced lettuce growth and photosynthetic performance with S supplementation, resulting in a 144.0% to 215.9% increase in shoot fresh mass in the two cultivars compared with the control. Thus, we recommend that at least 10 mg⋅L−1 of S should be supplemented when growing lettuce hydroponically to ensure optimal plant growth, especially when S is absent or low in the fertilizer and water source.

Review on Weight Loss Interventions that Can Prevent Muscle Mass Loss in Sarcopenic Obesity

Objectives: The objective of this study was to review clinical studies conducted over the last ten years that investigated weight or fat loss interventions that can preserve muscle or fat-free mass in Sarcopenic obesity Methods: PubMed, Embase, Cochrane Central Register of Controlled Trials (CENTRAL), Research Information Sharing Service (RISS) and Korea Studies Information Service (KISS) were searched for Randomized clinical trials that had investigated all-type of interventions on the management of sarcopenic obesity from October 2013 to September 2023.Results: A total of 14 studies met all the inclusion criteria. Interventions that increase muscle mass while reducing body fat at the same time included resistance training (including using elastic bands) and whole-body electromyostimulation(WB-EMS) in exercise intervention and Hypocaloric high-protein diet in nutritional intervention, exercise and nutritional combined intervention, and combination intervention of electrical acupuncture and amino acid supplementation. Among them, the most positive method of changing the body composition in sarcopenic obesity was the electric acupuncture and amino acid supplements.Conclusion: Varying diagnostic criteria and management interventions for sarcopenic obesity in the included studies made it hard to maintain homogeneity across the studies. Well-defined criteria for diagnostic sarcopenic obesity should be considered. In addition, since all of the interventions examined did not show sufficient clinical effectiveness, follow-up studies are needed to confirm effective interventions for sarcopenic obesity patients in the future.

CPPU Improves Fruit Setting and Growth in Greenhouse-grown Oriental Melons (Cucumis melo L. var. makuwa Makino)

The production of melons (Cucumis melo L.) in greenhouses relies on pollination. Extreme temperature and insufficient light reduce not only flower visitation by pollinators but also pollen viability, resulting in inefficient pollination. In this study, we investigated the effects of forchlorfenuron [(N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU)] on the fruit setting and growth of oriental melons (C. melo L. var. makuwa Makino). The primary objective was to devise a new strategy for the management of oriental melons. Treatment with 5 mg·L−1 CPPU a day before flowering (T1), on the day of flowering (T2), and a day after flowering (T3) increased the fruit setting rate (by 20.1% to 30%) in melons subjected to artificial pollination (AP) or no pollination (NP) compared with the rate in those subjected to only artificial pollination without CPPU (CK). CPPU treatment induced unfertilized seeds; in addition, a tendency toward parthenocarpy was noted. The highest fruit setting rate (∼95%) was noted in plants subjected to the following treatments: AP+T1 and NP+T3. The rates of abnormal fruit formation decreased from 45.2% of CK group to 9.4% in plants subjected to AP+T1 and to 19.4% in those subjected to NP+T3. Elevated exogenous concentrations of CPPU markedly increased fruit weight. Plants subjected to NP+10 mg·L−1 CPPU bore the heaviest fruits (541.0 g), which were heavier than those borne by plants subjected to AP+10 mg·L−1 CPPU. CPPU treatment reduced the fruit cavity ratio in a concentration-dependent manner from 47.3% to 33.6% and increased the pulp thickness from 1.5 to 2.5 cm. Notably, supplementary CPPU treatment exerted no significant effects on fruit traits. Regarding taste, inconsistent results were obtained for sugar accumulation. Although the content of cucurbitacin B increased immediately after the initial CPPU treatment, it markedly decreased after 15 days of CPPU treatment. Therefore, mature melons did not have a bitter taste.

Clinical efficacy and potential mechanism of ginseng polysaccharides in the treatment of non-small cell lung cancer based on meta-analysis associated with network pharmacology

BackgroundThe ginseng polysaccharide injection is a well-known traditional Chinese medicine often employed as a supplementary treatment for cancer. This treatment can not only alleviate the adverse effects caused by tumor radiotherapy and chemotherapy but also enhance the immune system of individuals diagnosed with lung cancer. It is important to acknowledge the efficacy of ginseng polysaccharide injection in the treatment of non-small cell lung cancer (NSCLC). However, these small-sample studies may have certain biases, and the underlying mechanisms of ginseng polysaccharides therapy for NSCLC are still unclear. MethodsThe present study involved a systematic review of the literature on randomized controlled trials (RCTs) focusing on using ginseng polysaccharide injection as a therapeutic approach for NSCLC. Seven databases were searched for eligible studies published before April 2023. Two researchers independently managed data extraction, risk of bias assessment, and data analyses using RevMan 5.3 software. In network pharmacology, we thoroughly searched the relevant literature on ginseng polysaccharides (GPs) and the PubChem database. This search aimed to identify the main active ingredients and targets associated with ginseng polysaccharides. Subsequently, we compared these targets with those of NSCLC and utilized bioinformatics techniques to analyze and explore their potential interactions. ResultsA total of 11 RCTs involving 845 patients with NSCLC were included in the meta-analysis. The meta-analysis revealed that ginseng polysaccharide injection combined significantly improved the objective response rate [RR = 1.45, 95% CI (1.26, 1.67), P < 0.00001]. Furthermore, it was observed that ginseng polysaccharide injection increased the serum levels of CD4+ T-lymphocytes (CD4+ T) [MD = 8.98, 95% CI (5.18, 12.78), P < 0.00001], and decreased the serum levels of CD8+ T-lymphocytes (CD8+ T) [MD = −2.68, 95% CI (−4.66, −0.70), P = 0.008]. Through network pharmacology analysis, a total of 211 target genes of GPs and 81 common targets were identified. GAPDH, EGFR, VEGFA, JUN, SRC, CASP3, STAT3, CCND1, HSP90AA1, and MMP9 were identified as the core target proteins. Additionally, KEGG enrichment analysis revealed 122 relevant signaling pathways, including Pathways in cancer, PD-L1 expression and PD-1 checkpoint pathway in cancer, and Proteoglycans in cancer. ConclusionGinseng polysaccharide injection can improve the ORR of patients with NSCLC, increase the serum levels of CD4+ T, and decrease the serum levels of CD8+ T. The potential mechanism may be associated with the PD-1/PD-L1 signaling pathway.

The role of microbiota on rheumatoid arthritis onset.

Rheumatoid arthritis (RA) is a chronic autoimmune disease that causes inflammation and pain, which can lead to the loss of normal joint function. Although the exact cause of the disease is not yet fully understood, both environmental factors and genetics may play a role in its development. Moreover, research suggests microbiota contributes to the onset and progression of RA. People with RA show higher quantities of bacteria such as Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Prevotella copri, Proteus mirabilis, and Lactobacillus salivarius compared to healthy individuals. Conversely, studies propose that Lactobacillus casei, a probiotic bacterium with immunomodulatory properties, has beneficial effects for RA in murine and human models. Therefore, this work reviews the potential role of the gut microbiota in the development of RA and explores the feasibility of using probiotic bacteria as a supplementary treatment for this disease.

Sorghum Response to Supplementary Irrigation at Different Growth Stage in Rain-Fed Agriculture for Increasing Yield and Water Productivity at Raya Valley

In arid and semi-arid ecosystems, the efficient use of water becomes increasingly important because water is a crucial limiting factor for increased food and fibre production to supply an ever-growing number of people. A field experiment was conducted in three consecutive seasons three year in Mekhoni Agricultural Research Centre, Raya valley, to investigate with the objective of to evaluate the influence of supplemental irrigation applied at different growth stages on the yield, to prevent sorghum failure caused by less rain water and to maximize the water productivity of the sorghum. The experiment was laid out in a random complete block design (RCBD) consisting of eleven supplemental irrigation treatments (one supplemental irrigation during initial stage, one supplemental during development stage, one supplemental during mid-stage, one supplemental during Late stage, one supplemental during initial and development stage, one supplemental during initial and mid stage, one supplemental during initial and late stage, one supplemental during development and late stage, one supplemental during development and late stage and rain-fed (with no SI) as a control treatment with three replications. The result was showed a significant difference on plant height, panicle length, grain, biomass yield and water productivity sorghum. The highest grain yield was obtained from the treatment supplement one SI at development and mid stage and followed by one SI at mid stage and at late stage. Whereas, the lowest grain yield of sorghum was observed from treatment with rainfed (non-supplementary). It is concluded that in the study area the rain fed amount were very inadequate and supplementary irrigation will help to increase crop productivity, minimize crop failure due to the low rainfed. The experiment showed that supplementary irrigation offers a great potential for improving crop production in Raya valley.

Probiotics Supplementation during Pregnancy: Can They Exert Potential Beneficial Effects against Adverse Pregnancy Outcomes beyond Gestational Diabetes Mellitus?

Probiotics, as supplements or food ingredients, are considered to exert promising healthy effects when administered in adequate quantity. Probiotics' healthy effects are related with the prevention of many diseases, as well as decreasing symptom severity. Currently, the most available data concerning their potential health effects are associated with metabolic disorders, including gestational diabetes mellitus. There is also clinical evidence supporting that they may exert beneficial effects against diverse adverse pregnancy outcomes. The purpose of the current narrative study is to extensively review and analyze the current existing clinical studies concerning the probable positive impacts of probiotics supplementation during pregnancy as a protective agent against adverse pregnancy outcomes beyond gestational diabetes mellitus. a comprehensive and thorough literature search was conducted in the most precise scientific databases, such as PubMed, Scopus, and Web of Sciences, utilizing efficient, representative, and appropriate keywords. in the last few years, recent research has been conducted concerning the potential beneficial effects against several adverse pregnancy outcomes such as lipid metabolism dysregulation, gestational hypertensive disorders, preterm birth, excessive gestational weight gain, caesarean risk section, vaginal microbiota impairment, mental health disturbances, and others. up to the present day, there is only preliminary clinical data and not conclusive results for probiotics' healthy effects during pregnancy, and it remains questionable whether they could be used as supplementary treatment against adverse pregnancy outcomes beyond gestational diabetes mellitus.

Evaluation of Weed Management Practices in Maize (&amp;lt;i&amp;gt;Zea mays&amp;lt;/i&amp;gt;) at Metu, Ethiopia

A field experiment was conducted at Metu Agricultural sub – centre on station in 2015 and 2016 cropping seasons to evaluate the effectiveness and economic feasibility of weed management methods on maize grain yield at Metu area. BH 661 at 25 kg/ha used as seed source. The experiment comprised of nine treatments laid out in randomized complete block design (RCBD) with three replications. The result revealed that the highest average grain yield 62.8 q/ha was obtained from Integrity EC 668g/l 1.0 liter with two supplementary hand weeding followed by 61.6 q/ha from Integrity EC 668g/l 1 liter with one hand weeding. Similarly, the minimum above ground wee dry biomass mean 0.07q/ha, 1.5 q/ha obtained from three hand weeding at 30, 60 and 90 days after planting and Integrity EC 668g/l 1.0 liter with two supplementary hand weeding respectively. Consequently highest weed control efficiency 99.93% and 98.5%obtained from the same treatment. In economic feasibility aspect the highest net benefit Accordingly the highest net benefit 61296.4 ETB ha&amp;lt;sup&amp;gt;-1 &amp;lt;/sup&amp;gt;was obtained from Integrity EC 668g/l at 1.0 L/ha + two time supplementary hand weeding with 3042.86 MRR% followed by 60146.2 ETB ha&amp;lt;sup&amp;gt;-1 &amp;lt;/sup&amp;gt;net benefit with 5661.90% MRR obtained from Integrity EC 668g/l at 1 l/ha + one times supplementary hand weeding whereas the minimum net benefit 50551.2 ETB ha&amp;lt;sup&amp;gt;-1&amp;lt;/sup&amp;gt; and 53224.2 ETB ha&amp;lt;sup&amp;gt;-1&amp;lt;/sup&amp;gt; obtained from one hand weeding at 30 &amp; 454 days after planting respectively. Therefore, Integrity EC 668g/l at 1 l/ha + two times supplementary hand weeding and Integrity EC 668g/l at 1 l/ha + one time supplementary hand weeding treatment consecutively more profitable weed management practices to control weeds in maize and there by improve crop production up to 56.94% than other treatments and can be recommended for maize production at Metu area.

Individuals Diagnosed With Type 2 Diabetes Mellitus and the Status of Vitamin B12 Deficiency: A Review.

Type 2 diabetes mellitus (T2DM) is a complex metabolic disorder with a multifactorial etiology and a significant global burden. In recent years, emerging evidence has suggested a potential link between T2DM and vitamin B12 deficiency, raising concerns about its impact on disease progression, management, and associated complications. This comprehensive review critically examines the current understanding of the prevalence, risk factors, clinical implications, and management strategies related to vitamin B12 deficiency in individuals diagnosed with T2DM. The review begins by providing an overview of the epidemiology of T2DM and its associated complications, underscoring the need for comprehensive management approaches. Subsequently, it delves into the physiology of vitamin B12, including its sources, absorption mechanisms, and biological functions, laying the groundwork for understanding the potential implications of deficiency in T2DM. A thorough analysis of the literature is conducted to elucidate the prevalence and risk factors of vitamin B12 deficiency in individuals with T2DM, considering factors such as age, duration of diabetes, medication use (e.g., metformin), dietary patterns, and comorbidities. Special attention is given to the role of metformin, the first-line therapy for T2DM, in precipitating or exacerbating vitamin B12 deficiency through mechanisms involving alterations in the gut microbiota and intestinal absorption. The review further explores the clinical manifestations and diagnostic challenges associated with vitamin B12 deficiency in the context of T2DM, emphasizing the importance of recognizing subtle symptoms and implementing appropriate screening protocols. It discusses the potential implications of vitamin B12 deficiency on glycemic control, diabetic neuropathy, cognitive function, cardiovascular health, and overall quality of life in individuals with T2DM. In addressing the management of vitamin B12 deficiency in T2DM, the review examines various therapeutic strategies, including oral and parenteral supplementation, dietary modifications, and lifestyle interventions. It critically evaluates the evidence supporting routine screening for vitamin B12 deficiency in individuals with T2DM and discusses controversies surrounding optimal supplementation protocols, dosing regimens, and monitoring strategies. Furthermore, the review highlights gaps in current knowledge and identifies areas for future research, such as the long-term effects of vitamin B12 supplementation on clinical outcomes in T2DM, the impact of genetic factors on vitamin B12 metabolism, and the potential role of personalized interventions. Overall, this review consolidates existing evidence and provides insights into the complex relationship between T2DM and vitamin B12 deficiency, aiming to inform clinical practice, enhance patient care, and guide future research endeavors in this important area of metabolic medicine.