Pressure injuries (PIs) are a significant concern in healthcare settings, often leading to severe complications for patients. Effective prevention relies heavily on the knowledge and attitudes of nursing staff. Understanding these factors can help formulate strategies to improve patient care and outcomes. Objective: To evaluate the knowledge and attitudes of nurses toward preventing pressure injuries and identify the factors influencing these parameters. Methods: A cross-sectional study was conducted in the Nursing Department of a Tertiary Care Hospital from May 2023 to May 2024. A total of 300 nurses working in the hospital for more than a year and without supervisory roles were selected using consecutive sampling. Data were collected through an anonymous questionnaire divided into demographic information, knowledge assessment using Pieper’s Pressure Ulcer Knowledge Test, and attitudes evaluation using the Attitude Towards Pressure Ulcer Prevention Instrument. Statistical analysis was performed using descriptive statistics and appropriate tests to assess the relationships between variables. Results: The average knowledge score among nurses was 32.88 ± 2.49, with a score of 6.10 ± 1.12 for PI evaluation and 26.83 ± 2.20 for prevention and grading. 59% of participants demonstrated good knowledge. The average attitude score was 39.90 ± 3.81, with the highest score in the personal role subgroup (7.91 ± 0.99) and the lowest in personal efficacy (9.84 ± 1.09). 54% of participants exhibited a favourable attitude towards PI prevention. Conclusion: Nurses displayed satisfactory knowledge and attitudes toward preventing pressure injuries. Enhancing these parameters through exemplary leadership, quality education, and regular training programs can improve healthcare outcomes and patient safety.