The superovulatory responses and embryo recoveries in Rathi cows superovulated with different doses of Folltropin-V were tested. Post-partum cows were superovulated 60 days post-partum using 400, 300, 200 or 240 mg of Folltropin-V administered in 8 descending doses 12 h IM starting from day 8–10 of a CIDR-B. Cows were induced to estrus by IM administration of a prostaglandin (Inj Cyclix) and withdrawal of CIDR-B at the time of last injection of FSH. Cows were inseminated thrice at 12 h interval using frozen semen. Embryos were recovered by non-surgical means on day seven of insemination. Transrectal ultrasonography was performed on the day of embryo recovery to record the number of unovulated follicles and the number of corpora lutea. Estrus expression was poor in superovulated donors which posed a difficulty in precise timing of insemination. The total ovulatory responses and the total embryo recoveries were non-significantly different between all the groups. However, the mean number of transferable grade embryos was significantly higher in the 300 mg group compared to all other groups. A large number of unovulated follicles were visible ultrasonographically on the day of estrus in the 400 mg group. A total of 2, 5, 3 and 2 animals yielded no embryos in 400, 300, 200 and 240 mg groups respectively and in a total of 5 animals the flushing catheter could not be traversed from the cervix. Transfer of embryos to 29 recipients did not result in pregnancy. It was concluded that superovulation and embryo recoveries are difficult in Rathi (Bos indicus) cows due to anatomic differences and that 300 mg of Folltropin-V appears to be the optimum dose for induction of superovulation.