Conception rates after artificial insemination or embryo transfer in lactating dairy cows during summer in florida

Abstract

The objective was to compare conception rates to embryo transfer relative to AI, during summer heat stress, in lactating dairy cows. Holstein cows (n=180; 50 to 120 d postpartum) were allocated randomly to 1 of 3 groups: artificial insemination (AI, n=84), embryo transfer using either embryos collected from superovulated donors (ET- DON, n=48), or embryos produced in vitro (ET-IVF, n=48). Embryos from superovulated donors were frozen in 10 % glycerol and were rehydrated in a 3-step procedure, in decreasing concentrations of glycerol in a sucrose medium before transfer. Embryos produced in vitro were frozen in 1.5 M ethylene glycol, thawed and transferred without rehydration. Blood samples were collected from AI and ET recipients on Days 0, 7 and 22 for measurement of progesterone in plasma. Conception rate was estimated for the three groups at Day 22 (progesterone>1 ng/mL) and confirmed at Day 42 by palpation per rectum. Conception rate estimates at Day 22 did not differ among groups (AI, 60.7%; ET-DON, 60.4%; ET-IW, 54.2%), but conception rates at Day 42 differed (AI, 21.4%; ET-DON, 35.4%; ET-IW, 18.8%; AI versus ET: P>0.10 and ET-DON versus ET-IVF: P<0.05). In cows considered pregnant at 22 d but diagnosed open at 42 d, the interestrous intervals were 28.8± 2.2, 35.2± 3.5 and 31.6±2.9 d, respectively, for AI, ET-DON and ET-IVF groups. Transfer of embryos collected from nonheat-stressed superovulated donors significantly increased conception rates in heat stressed dairy cattle. However, transfer of IVF-derived embryos had no advantage over AI. Where appropriate mechanisms are in place to attenuate the effects of heat stress, embryo transfer using frozen-thawed donor embryos increases conception rates.