Superior Strains Research Articles

High-throughput screening and identification of lignin peroxidase based on spore surface display of Bacillus subtilis.

Lignin peroxidase is closely related to agriculture and food as it improves the quality of feedstuffs, facilitates the degradation of lignin in agricultural wastes, and degrades azo dyes that have similar complex structures to lignin. However, the current status of homologous or heterologous expression of lignin peroxidase is unsatisfactory and needs to be modified with the help of immobilization and directed evolution to maximize its potential. Directed evolution technology is an effective strategy for designing and improving enzyme characteristics, and Bacillus subtilis spore surface display technology is an efficient method for preparing immobilized enzymes. A colorimetric dye decolorization assay using Congo red as a substrate was developed and optimized for high-throughput screening of spore surface display in a 96-well plate. After two rounds of screening, a superior mutant strain was selected from 2700 mutants. Its highest catalytic activity was 196.36%. The amino acid substitution sites were identified as N120D and I242T. The mechanism for the enhanced catalytic activity was explained using protein modeling and functional analysis software. This study provides insights into the rational design of lignin peroxidase and its application in food and agriculture. © 2024 Society of Chemical Industry.

Inoculation of multi-metal-resistant Bacillus sp. to a hyperaccumulator plant Sedum alfredii for facilitating phytoextraction of heavy metals from contaminated soil

Co-contamination of soil by multiple heavy metals is a significant global challenge. An effective strategy to address this issue involves using hyperaccumulators such as Sedum alfredii (S. alfredii). The efficiency of phytoremediation can be improved by supplementing with plant growth-promoting bacteria (PGPB). However, bacteria resources of PGPB resistant to multi-heavy metal contamination are still lacking. This study focused nine different strains of Bacillus and screened for resistance to heavy metals including cadmium (Cd), zinc (Zn), copper (Cu), and lead (Pb). A superior strain, Bacillus subtilis PY79 (B. subtilis), showed tolerance for all tested metals. Inoculation with B. subtilis in the rhizosphere of S. alfredii increased the accumulation of Cd, Zn, Cu, and Pb by 88.02%, 58.99%, 90.22%, and 54.97% in the plant shoots after 30 days respectively. B. subtilis application lowered the pH of the rhizosphere soil, thereby increasing the bioavailability of nutrients and heavy metals. Furthermore, B. subtilis helped S. alfredii recruit PGPB and heavy metal-resistant bacteria such as Edaphobacter, Niastella, and Chitinophaga, enhancing the growth and phytoremediation efficiency. Moreover, inoculation with B. subtilis not only upregulated genes of the ABC, HMA, ZIP, and MTP families involved in the translocation and detoxification of heavy metals but also increased the secretion of antioxidants within the cells. These findings indicate that B. subtilis enhances the tolerance, uptake, and translocation of heavy metals in S. alfredii, offering valuable insights for the phytoremediation of multi-metal-contaminated soils.

Cryogenic tribological behavior of coarse, ultrafine grained and heterogeneous Fe-18Cr-8Ni austenitic stainless steel

Commonly used austenitic stainless steels (ASSs) have some limitation in sliding wear conditions due to their relatively low yield strength and hardness. To improve the wear resistance, three kinds of microstructure (coarse grain (CG), heterogeneous structure (HS), and ultrafine grain (UFG)) are prepared, to investigate the grain size on the dry sliding tribological behavior, as well as wear mechanisms of Fe-18Cr-8Ni ASSs at room temperature (RT) and cryogenic temperature (−120 °C). The results indicate that at RT the UFG specimen exhibits the lowest wear rate during the wear tests, where the wear mechanisms are mainly oxidation wear and abrasive wear. While, when tested at −120 °C, the CG specimen exhibits the best wear resistance compared with that of the other two specimens due to its superior plastic deformation ability and strain hardening ability. Moreover, the HS specimen exhibits the lowest coefficient of friction (CoF), which is due to the abrasive particles generated on the contact surface provide a certain level of friction reduction, while the wear rate increases as these particles serve as third-party abrasives, which further removing material.

Nanofilament organization in highly tough fibers based on lamin proteins

The escalating plastic pollution crisis necessitates sustainable alternatives, and one promising solution involves replacing petroleum-based polymers with fibrous proteins. This study focused on the recombinant production of intracellular fibrous proteins, specifically Caenorhabditis elegans lamin (Ce-lamin). Ce-lamins spontaneously organize within the cell nucleus, forming a network of nanofilaments. This intricate structure serves as an active layer that responds dynamically to mechanical strain and stress. Herein, we investigated the arrangement of nanofilaments into nanofibrils within wet-spun Ce-lamin fibers using alcoholic solutions as coagulants. Our goal was to understand their structural and mechanical properties, particularly in comparison with those produced with solutions containing Ca+2 ions, which typically result in the formation of nanofibrils with a collagen-like pattern. The introduction of ethanol solutions significantly altered this pattern, likely through rearrangement of the nanofilaments. Nevertheless, the resulting fibers exhibited superior toughness and strain, outperforming various synthetic fibers. The significance of the nanofilament structure in enhancing fiber toughness was emphasized through both the secondary structure transition during stretching and the influence of the Q159K point mutation. This study improves our understanding of the structural and mechanical aspects of Ce-lamin fibers, paving the way for the development of eco-friendly and high-quality fibers suitable for various applications, including medical implants and composite materials.

Stress-induced martensite reorientation and its related performances in trained β-Ti based shape memory alloy

In the present study, the influence of training on the microstructural evolution, martensitic transformation behavior and mechanical/functional properties of Ti–V–Al–Zr shape memory alloy was investigated systematically. With the increasing of training cycling number, the degree of martensite reorientation became larger and larger. Moreover, higher density of dislocations appeared in the interior of martensite variants, upon the excessive training cycling number was applied. The international stress can be brought into the matrix, which not only promoted to the ω→αˊˊ transition, but also made the martensite phase stabilizer and even suppressed the αˊˊ→β martensitic transformation during training process. The superior mechanical properties including maximum fracture stress of 906 MPa and the higher fracture strain of 33.6% can be obtained in Ti–V–Al–Zr shape memory alloy subjected with the training cycles number of 5th, which can be attributed to the matrix strengthening stemmed from work hardening and suppression of ω precipitation. Meanwhile, the lower critical stress for the martensite variant reorientation and enhanced matrix strength synergistically contributed to the superior strain recovery characteristics with total completely recoverable strain of 6% in Ti–V–Al–Zr shape memory alloy by controlling training cycles number of 5th. Meanwhile, the higher micro-hardness can be obtained by training.

Thermo-kinetic insights into deformation mechanism in the phase-transforming nanostructured Fe alloy

Solid-state phase transformations (SSPTs) being the most widely versatile routes to tailor microstructures are less utilized in the design of nanostructured (NS) alloys, resulting in a lack of understanding of the significance of SSPTs in tuning mechanical properties. Here, we combine nanostructuring with reverse austenite transformation to make a phase-transforming NS Fe alloy consisting of ultrafine ferrite and austenite grains. By comparison with the coarse-grained (CG) counterpart, the NS sample exhibits high strengths (i.e., the yield and the ultimate tensile strengths are 816 and 1170 MPa, respectively), good ductility with a uniform elongation of 53 %, and superior strain hardening capacity, under multiple strengthening mechanisms and the activation of transformation induced plasticity. Regarding the mechanism in forming the NS sample, grain refinement is revealed to markedly affect the austenite formation kinetics by generating sluggish growth behavior accompanied by sufficient solute partitioning. Then, we clarify the difference in mechanical responses of the phase-transforming NS and CG samples using a physically based microstructures-properties model. Further based on the thermo-kinetic theory of generalized stability, we show that the simultaneous increase of strength and ductility in the NS sample relative to the CG sample is originated from the deformation physics with higher driving force and higher generalized stability. From the thermo-kinetic perspective, our work demonstrates that the SSPTs hold substantial promise in optimizing the microstructures and the mechanical properties of NS Fe alloys and are expected to find wide application in the development of other NS materials.

Flexible Exchange‐Biased Films with Superior Strain Stability

AbstractThe exchange bias (EB) effect plays an undisputed role in the state‐of‐art spintronic devices, both rigid and flexible. However, the poor stability of EB under mechanical strain is detrimental to the construction of reliable flexible spintronic devices, which poses a great challenge for potential wearable applications. Here, it is revealed that the strain‐induced variation of EB field can be greatly suppressed by engineering flexible IrMn/[Co/Pt]3 systems featured with perpendicular magnetic anisotropy (PMA). Particularly, in the multi‐stacks with strong PMA, poorly crystallized IrMn structure, and parallel alignment of the ferromagnetic (FM) moments with the biasing magnetic field applied during deposition, an exceptional good strain stability of EB among reported flexible systems is realized, which results from the stable perpendicular FM moments, the low strain transfer efficiency, and the eliminated strain‐driven training effect. These results provide an effective approach to develop flexible spintronic devices with superior strain stability, rendering them strong contenders in the realms of sensor and storage applications.

Spin-coated BiFeO3 films on Si wafers: Low processing temperature but prominent piezoelectricity

The direct integration of crystalline oxide layers with industrial Si substrate, specifically compatible with CMOS technology, requires the development of relatively simple, low-temperature processing routes below 450 °C. Here, a novel nonstoichiometric approach is proposed to achieve fabrication of BiFeO3 films at 450 °C. Of particular importance is that, a saturation and remnant polarization of ∼80 μC/cm2 and ∼60 μC/cm2 and a strain as large as 1% are obtained. This strain stands as one of the most impressive values reported for thin films, comparable to the most superior strain obtained in ferroelectric films fabricated at temperatures exceeding 700 °C. The current work provides a new paradigm with significant simplicity and novel efficacy in reducing processing temperatures, as well offers a promising material for memory and piezo-driven actuating applications, especially meeting the increasing demand for precision position control systems at the nanometer scale.

STRAIN TRANSFER ANALYSIS OF A FOUR-LAYER EMBEDDED FIBER OPTIC SENSING MODEL IN AN ELASTIC STATE

Optical fiber grating strain sensors are currently utilized in a variety of structural health monitoring applications. The encapsulated fiber optic sensor is unable to completely detect the strain of the structure, so the strain transfer theory should be established to maximize the strain sensing of fiber. It is required to explore the embedded four-layer fiber optic sensing model to create a more plausible strain transfer error hypothesis. Based on the three-layer fiber optic sensing model, the Goodman assumption and Fourier series approach were presented to study the strain transfer efficiency of the four-layer model in the elastic state. First, the physical quantity to be analyzed is determined, and finally the radius, interlayer bonding coefficient and elastic modulus are selected as the parameters affecting the strain transfer efficiency. The length range of efficiency evaluation is 0–2.5[Formula: see text]m, and the transfer efficiency under different radii is above 0.90 when the length [Formula: see text][Formula: see text]m. The interlayer bonding coefficient [Formula: see text] between [Formula: see text][Formula: see text]N/m3 and [Formula: see text][Formula: see text]N/m3 has little impact on the transfer efficiency, and the same is true for [Formula: see text], so it cannot be considered in practice. When [Formula: see text] is between [Formula: see text][Formula: see text]N/m3 and [Formula: see text][Formula: see text]N/m3 and the length [Formula: see text][Formula: see text]m, the strain transfer coefficient reaches 95%. The influence of elastic modulus on the transfer efficiency is very significant when [Formula: see text][Formula: see text]m. The four-layer model performs similarly to the three-layer model within the paste length range of 1.0[Formula: see text]m, but has a superior strain transfer effect when the pasted length exceeds 1.0[Formula: see text]m. As the radius of the protective layer rises, the effect of strain transfer deteriorates.

In-situ high-temperature micromechanical behavior of flash-sintered strontium titanate

Strontium titanate (SrTiO3) is a versatile material with various applications but understanding its mechanical properties, especially in polycrystalline form prepared via field-assisted sintering methods, is limited. This study investigates the high-temperature mechanical properties of flash-sintered SrTiO3 through in-situ microcompression tests comparing the behaviors near the positive and negative electrodes. Due to a significant irregularity in densification, the negative electrode exhibited superior fracture strength and strain across all temperatures when compared to its positive counterpart. Micropillars near the positive electrode contained multiple pre-existing pores that became crack initiation sites, and thus inducing catastrophic failure. Micropillars near the negative electrode exhibited prominent intergranular cracks accompanied by high-density dislocations in the vicinity of the fracture surfaces. This study elucidates the effect of flash sintering-induced defects on the mechanical properties of polycrystalline SrTiO3 at various temperatures.

Influence of amine chain extenders on morphology and performances of poly(dimethylsiloxane) based poly(urea‐urethane) elastomers

AbstractThe structure, morphology, and performance of polyurethanes are greatly influenced by the structure of chain extenders. In this study, two types of amine chain extenders, 1,3‐bis(3‐aminopropyl)‐1,1,3,3‐tetramethyldisiloxane (BAPD) and isophorone diamine (IPDA), were utilized in the synthesis of poly(dimethylsiloxane) (PDMS)‐based poly(urea‐urethane) elastomers (PDMS‐PUUs). The mechanical properties, morphology, and in vitro oxidative stability of the as‐prepared elastomers were controlled by altering the ratio of BAPD and IPDA. The results indicate that the overall phase mixing of PDMS‐PUUs improves after incorporating of BAPD into the hard segments. The hard segment, composed of IPDA with a rigid structure, plays a crucial role in maintaining the essential mechanical properties of the PDMS‐PUUs. Furthermore, PDMS‐PUUs based on mixed chain extenders exhibit relatively high boundary diffuseness and boundary thickness. Especially, the PDMS‐PUU sample synthesized from BAPD and IPDA with a molar ratio of 1/1 features the highest boundary diffuseness (0.094) and boundary thickness (0.395 nm), consequently displaying some remarkable properties, including enhanced cyclic tensile properties, superior strain recovery (92.6%), a low modulus (8.6 MPa), and high tensile strength (20.3 MPa). PDMS‐PUUs also exhibit good oxidative stability and biocompatibility, implying that the as‐prepared PDMS‐PUUs are proven to be biostable and capable of long‐term implantation.

Ultrasensitive electrospinning fibrous strain sensor with synergistic conductive network for human motion monitoring and human-computer interaction

With the rapid development of wearable electronic skin technology, flexible strain sensors have shown great application prospects in the fields of human motion and physiological signal detection, medical diagnostics, and human-computer interaction owing to their outstanding sensing performance. This paper reports a strain sensor with synergistic conductive network, consisting of stable carbon nanotube dispersion (CNT) layer and brittle MXene layer by dip-coating and electrostatic self-assembly method, and breathable three-dimensional (3D) flexible substrate of thermoplastic polyurethane (TPU) fibrous membrane prepared through electrospinning technology. The MXene/CNT@PDA-TPU (MC@p-TPU) flexible strain sensor had excellent air permeability, wide operating range (0–450 %), high sensitivity (Gauge Factor, GFmax = 8089.7), ultra-low detection limit (0.05 %), rapid response and recovery times (40 ms/60 ms), and excellent cycle stability and durability (10,000 cycles). Given its superior strain sensing capabilities, this sensor can be applied in physiological signals detection, human motion pattern recognition, and driving exoskeleton robots. In addition, MC@p-TPU fibrous membrane also exhibited excellent photothermal conversion performance and can be used as a wearable photo-heater, which has far-reaching application potential in the photothermal therapy of human joint diseases.

Unlocking the secrets of intraspecific hybrids (Vitis vinifera L.) cold hardiness: A comprehensive study of genetic factors and trait correlations

Challenges arise in global viticulture due to low temperatures. To ensure the sustainable and high-quality development of the wine industry, it is essential to breed wine grape varieties that are not only of high quality but also possess cold hardiness. Intraspecific recurrent selection in Vitis vinifera can enhance cold hardiness while maintaining fruit quality. In this study, we used ‘Ecolly ’as an intermediary grape variety for crossing with ‘Cabernet Sauvignon’, ‘Marselan’, and ‘Dunkelfelder’, including three reciprocal crosses and a total of 1,657 intraspecific hybrids. We characterized the cold hardiness of these intraspecific hybrids and analyzed the genetic aspects of cold hardiness, ultimately identifying excellent strains with cold hardiness. Parameters like mean high-temperature exotherm (mHTE), mean low-temperature exotherm (mLTE), bound/free water ratio, water loss ratio in vitro, frost damage grades, and overall performance displayed partially normal distributions. In intraspecific hybrids, there was a maternal advantage in traits related to bound/free water ratio and water loss ratio. Some hybrid populations exhibited values for mHTE, mLTE, and water loss ratio that were lower than the low parent's values, while bound/free water ratio showed values higher than the high parent's values. Among the 1,657 intraspecific hybrids, 52 strains could bud under stress at −18 °C, and seven of these strains excelled in three important cold hardiness measures. Our study revealed that cold hardiness in V. vinifera is influenced by multiple genes and is a quantitative trait. Intraspecific hybridization can produce a small number of superior strains with enhanced cold hardiness.

Multi-scale annealing twins generate superior ductility in an additively manufactured high-strength medium entropy alloy

Coherent twin boundaries (CTBs) are internal planar defects that offer a promising pathway for designing advanced metallic materials with superior strength-ductility synergy. However, incorporating nanoscale CTBs into additive manufacturing (AM) microstructures is highly challenging without severe plastic deformation. Here, by utilizing the intrinsic cellular structures in AM alloys, we for the first time achieved a high density of multi-scale annealing twins in a laser powder bed fusion (LPBF) Ni35Co35Cr25Ti3Al2 medium-entropy alloy. These multi-scale annealing twins, together with nanoprecipitates and dislocations, resulted in gigapascal strength (∼1.4 GPa) and substantial tensile ductility (∼25 %). We reveal that the AM-induced cellular structures, decorated with entangled dislocations and Ti segregation at the cellular boundaries, facilitate the abundant nucleation of multi-scale annealing twins through interactions with migrating recrystallization boundaries. Additionally, the cellular precipitation networks enhance the thermal stability of nanoscale annealing twins. Frequent dislocation-TB interactions during deformation contribute to superior strain hardenability and thus good ductility. Synergized multiple strengthening mechanisms, i.e., boundary strengthening, precipitation strengthening, and dislocation strengthening, are responsible for the excellent strength. Our present findings advance the design of AM microstructures by harnessing the beneficial effects of cellular structures and provide valuable guidance for developing alloys with exceptional mechanical properties.

Bifidobacterium longum subsp. longum BG-L47 boosts growth and activity of Limosilactobacillus reuteri DSM 17938 and its extracellular membrane vesicles.

By using probiotics that contain a combination of strains with synergistic properties, the likelihood of achieving beneficial interactions with the host can increase. In this study, we first performed a broad screening of Bifidobacterium longum subsp. longum strains in terms of synergistic potential and physiological properties. We identified a superior strain, BG-L47, with favorable characteristics and potential to boost the activity of the known probiotic strain Limosilactobacillus reuteri DSM 17938. Furthermore, we demonstrated that BG-L47 is safe for consumption in a human randomized clinical study and by performing a genome safety assessment. This work illustrates that bacteria-bacteria interactions differ at the strain level and further provides a strategy for finding and selecting companion strains of probiotics.

Structural characterization and mechanical properties of bicontinuous dual-metal composites prepared by liquid metal dealloying

Most bicontinuous dual-metal composites (BDMCs) generated by liquid metal dealloying (LMD) exhibit lamellar microstructures at the grain boundaries of initial precursor alloys, resulting in little to no ductility. Herein, grain-boundary-defect-free bicontinuous Ni/Ag composites consisting of network-like dealloying structures (Ni phase) and solidified matrices of liquid metal corrosive media (Ag phase) are prepared. Then, a detailed study of their intrinsic structures and mechanical properties under uniaxial tension is conducted. This composite is found to be completely dense without visible holes or cracks. Both the Ni phase and Ag phase have independent open porous structures and are well bonded with clear interfaces, forming a dual-interlocking structure with phase-to-phase and grain-to-grain interlocking. This structure does not significantly affect the elastic modulus or tensile yield strength, but under specific conditions, it can enhance the tensile ductility because of the coordinated deformation between the two phases, thereby reducing the likelihood of stress concentration and delays necking. Moreover, these bicontinuous Ni/Ag composites display superior strain hardening rates in comparison to pure Ag. The high strength and ductility of this structure warrant the exploration of its practical application in many areas, if more defect-free systems are synthesized by optimizing the LMD process in the future.

Exploring tungsten-oxygen vacancy synergy: Impact on leakage characteristics in Hf0.5Zr0.5O2 ferroelectric thin films

The recent discovery of ferroelectric properties in HfO2 has sparked significant interest in the fields of nonvolatile memory and neuromorphic computing. Yet, as device scaling approaches sub-nanometer dimensions, leakage currents present a formidable challenge. While tungsten (W) electrodes are favored over traditional TiN electrodes for their superior strain and interface engineering capabilities, they are significantly hampered by leakage issues. In this study, we elucidate a positive feedback mechanism attributable to W electrodes that exacerbates oxygen vacancy defects, as evidenced by density functional theory computations. Specifically, intrinsic oxygen vacancies facilitate the diffusion of W, which, in turn, lowers the formation energy of additional oxygen vacancies. This cascade effect introduces extra defect energy levels, thereby compromising the leakage characteristics of the device. We introduce a pre-annealing method to impede W diffusion, diminishing oxygen vacancy concentration by 5%. This reduction significantly curtails leakage currents by an order of magnitude. Our findings provide a foundational understanding for developing effective leakage suppression strategies in ferroelectric devices.

Genomic approachesidentifySTT4 as a new component in glucose-induced activation of yeast plasma membrane H+-ATPase

Many studies have focused on identifying the signaling pathway by which addition of glucose triggers post-translational activation of the plasma membrane H+-ATPase in yeast. They have revealed that calcium signaling is involved in the regulatory pathway, supported for instance by the phenotype of mutants inARG82 that encodes an inositol kinase that phosphorylates inositol triphosphate (IP3). Strong glucose-induced calcium signaling, and high glucose-induced plasma membrane H+-ATPase activation have been observed in a specific yeast strain with the PJ genetic background. In this study, we have applied pooled-segregant whole genome sequencing, QTL analysis and a new bioinformatics methodology for determining SNP frequencies to identify the cause of this discrepancy and possibly new components of the signaling pathway. This has led to the identification of an STT4 allele with 6 missense mutations as a major causative allele, further supported by the observation that deletion of STT4 in the inferior parent caused a similar increase in glucose-induced plasma membrane H+-ATPase activation. However, the effect on calcium signaling was different indicating the presence of additional relevant genetic differences between the superior and reference strains. Our results suggest that phosphatidylinositol-4-phosphate might play a role in the glucose-induced activation of plasma membrane H+-ATPase by controlling intracellular calcium release through the modulation of the activity of phospholipase C.

Cell envelope and stress-responsive pathways underlie an evolved oleaginous Rhodotorula toruloides strain multi-stress tolerance

BackgroundThe red oleaginous yeast Rhodotorula toruloides is a promising cell factory to produce microbial oils and carotenoids from lignocellulosic hydrolysates (LCH). A multi-stress tolerant strain towards four major inhibitory compounds present in LCH and methanol, was derived in our laboratory from strain IST536 (PYCC 5615) through adaptive laboratory evolution (ALE) under methanol and high glycerol selective pressure.ResultsComparative genomic analysis suggested the reduction of the original strain ploidy from triploid to diploid, the occurrence of 21,489 mutations, and 242 genes displaying copy number variants in the evolved strain. Transcriptomic analysis identified 634 genes with altered transcript levels (465 up, 178 down) in the multi-stress tolerant strain. Genes associated with cell surface biogenesis, integrity, and remodelling and involved in stress-responsive pathways exhibit the most substantial alterations at the genome and transcriptome levels. Guided by the suggested stress responses, the multi-stress tolerance phenotype was extended to osmotic, salt, ethanol, oxidative, genotoxic, and medium-chain fatty acid-induced stresses.ConclusionsThe comprehensive analysis of this evolved strain provided the opportunity to get mechanistic insights into the acquisition of multi-stress tolerance and a list of promising genes, pathways, and regulatory networks, as targets for synthetic biology approaches applied to promising cell factories, toward more robust and superior industrial strains. This study lays the foundations for understanding the mechanisms underlying tolerance to multiple stresses in R. toruloides, underscoring the potential of ALE for enhancing the robustness of industrial yeast strains.

Kinetic studies on optimized extracellular laccase from Trichoderma harzianum PP389612 and its capabilities for azo dye removal

BackgroundAzo dyes represent a common textile dye preferred for its high stability on fabrics in various harsh conditions. Although these dyes pose high-risk levels for all biological forms, fungal laccase is known as a green catalyst for its ability to oxidize numerous dyes.MethodsTrichoderma isolates were identified and tested for laccase production. Laccase production was optimized using Plackett–Burman Design. Laccase molecular weight and the kinetic properties of the enzyme, including Km and Vmax, pH, temperature, and ionic strength, were detected. Azo dye removal efficiency by laccase enzyme was detected for Congo red, methylene blue, and methyl orange.ResultsEight out of nine Trichoderma isolates were laccase producers. Laccase production efficiency was optimized by the superior strain T. harzianum PP389612, increasing production from 1.6 to 2.89 U/ml. In SDS-PAGE, purified laccases appear as a single protein band with a molecular weight of 41.00 kDa. Km and Vmax values were 146.12 μmol guaiacol and 3.82 μmol guaiacol/min. Its activity was stable in the pH range of 5–7, with an optimum temperature range of 40 to 50 °C, optimum ionic strength of 50 mM NaCl, and thermostability properties up to 90 °C. The decolorization efficiency of laccase was increased by increasing the time and reached its maximum after 72 h. The highest efficiency was achieved in Congo red decolorization, which reached 99% after 72 h, followed by methylene blue at 72%, while methyl orange decolorization efficiency was 68.5%.ConclusionTrichoderma laccase can be used as an effective natural bio-agent for dye removal because it is stable and removes colors very well.