Background: Staphylococcal food poisoning (SFP) is one of the most common food-borne diseases worldwide resulting from the contamination of food by preformed S. aureus enterotoxins and milk is considered to be a major source of SFP. This study aimed at the PCR based detection of S. aureus and its enterotoxigenic genes in raw milk and milk products. Methods: A total of 300 samples of raw milk and milk products were collected randomly from Aizawl district of Mizoram and were subjected to phenotypic and genotypic (nuc gene) detection of S. aureus and subsequent detection of enterotoxigenic genes namely, sea, seb, sec and sed in S. aureus isolates Result: A proportion of 30.67% S. aureus isolates were found to be positive for species specific nuc gene and 28.26%, 2.17%, 4.34% and 5.43% S. aureus strains were positive for sea, seb, sec and sed genes, respectively. Most contamination of raw milk was observed with S. aureus harbouring highest proportion of sea gene. Combination of two enterotoxigenic genes, sea with another gene, was also possessed by the S. aureus isolates from milk and milk products.