Sunflower Oil Research Articles

Optimization of ultrasound-assisted extraction of phenols from Crocus sativus by-products using sunflower oil as a sustainable solvent alternative

In the last decade, there's been a rising emphasis on eco-friendly solvents in industry and academia due to environmental concerns. Vegetable oils are now recognized as a practical, non-toxic option for extracting phytochemicals from herbs. This study presents a novel, green, and user-friendly method for extracting phenolic content from Crocus sativus L. waste using ultrasound. It replaces conventional organic solvents with sustainable sunflower oil, making the process eco-friendly and cost-effective. The effects of temperature (18–52 °C), ultrasonic time (5–55 min), and solid-solvent ratio (5–31 g/100 mL) were assessed by applying response surface methodology (RSM) and Central composite design. The combined impact of solid-solvent ratio, temperature, and ultrasonic time led to heightened phenolic content and antioxidant activity in the enriched oil. However, when these variables were at their maximum levels, there was a decline in these attributes. The specific conditions found to be ideal were a solid-to-liquid ratio of 26 g/100 mL, a temperature of 45 °C, and a duration of 45 min. The optimum extraction condition yielded the expected highest phenolic content (317.15 mg/ Kg), and antioxidant activity (89.34%). The enriched oil with flower saffron enabled the utilization of renewable natural ingredients, ensuring the production of a healthy extract or product. Also, enriched oils find diverse applications in areas such as food, aquaculture, and cosmetics.

Replacing non-renewable lubricants with vegetables oils in threaded joints

Lubrication is essential to ensure the proper performance of threaded joints subjected to multiple tightenings. Previous research has investigated the effectiveness of various mineral and synthetic lubricants, but no studies have been conducted on those derived from renewable sources. In this study, the performances of sesame, sunflower, coconut, and castor oil are compared to traditional VG46 oil and MoS2 grease. First, the rheological properties of the oils have been characterized. Then, tightening tests have been carried out to measure the coefficients of friction at the underhead and thread. The results demonstrate that vegetable oils outperform mineral VG46, especially in terms of repeatability. In particular, fractionated coconut oil exhibits exceptionally low coefficients of friction, which are not influenced by the tightening speed, unlike all other tested lubricants.

Phosphatidylcholine-depleted lecithin as low-HLB emulsifier in double emulsions: A sensitivity analysis

Phosphatidylcholine-depleted (PC-depleted) lecithin is characterized by a lower phosphatidylcholine/phosphatidylethanolamine (PC/PE) ratio, favoring the formation of water-in-oil (W/O) emulsions, and thus can be applied in water-in-oil-in-water (W/O/W) double emulsions (DEs). However, up to now, only a limited number of studies have described the use of PC-depleted lecithin in double emulsions. In this study, the influence of the formulation and processing conditions on the formation and stability of PC-depleted lecithin stabilized-DEs was investigated. The characterizations of DEs involved morphology, droplet size, viscosity, and entrapped water yield. The experimental results indicated that the incorporation of glucose in the water phase instead of potassium chloride (KCl) increased both the droplet size and the entrapped water yield in DEs, whereby the latter was increased by around 65% as compared to DEs containing 100 mM KCl. Reducing the salt content also facilitated the formation of DEs. Moreover, a correlation between the oil type and the 1st homogenization method was found: whereas DEs formulated with high oleic sunflower oil were always unstable irrespective of the emulsification method used, stable DEs were produced by the combination of medium-chain triglyceride oil and a rotor-stator system whereby microfluidization caused phase inversion. In addition, PC-depleted lecithin could stabilize up to 60% of water in oil. Meanwhile, stable DEs with a maximum internal water fraction of 24.1% were obtained by adjusting the original W/O/W ratio to 25/25/50. Finally, DEs stabilized by PC-depleted lecithin showed a comparable stability to those stabilized by polyglycerol polyricinoleate (PGPR) during 28 days of storage. To sum up, this work provides a better understanding of the sensitivity of PC-depleted lecithin-stabilized W/O/W emulsions towards the applied formulation conditions.

Peach Palm Fruit (Bactris gasipaes) Peel: A Source of Provitamin A Carotenoids to Develop Emulsion-Based Delivery Systems.

The peach palm fruit (Bactris gasipaes) peel is a byproduct after fruit consumption. The peel flour of two varieties (yellow and red) was separately obtained by hot air drying and was subsequently milled. The proximate analysis showed that the red variety exhibited higher protein, fat, and fiber contents than the yellow one. A higher carotenoid (836.5 ± 24.5 μg/g), phenolic compounds (83.17 ± 1.76 mg GAE/100 g), and provitamin A activity (33.10 ± 0.83 μg retinol/g) were found in the cooked red variety. The carotenoid and phenolic compositions were analyzed by HPLC-PDA-MS, finding β-carotene and γ-carotene to be major compounds. The effect of thermal treatment increased the amount of these provitamin A carotenoids and lycopene, which were detected only in the red variety. Among phenolic compounds, procyanidin dimer (isomer I), feruloyl quinic acid, and several apigenin C-hexosides were identified as major constituents of peach palm epicarp. A carotenoid-rich emulsion-based delivery system was obtained after the optimization (RSM model) of carotenoid extraction with ultraturrax and sunflower oil and further development of an ultrasound-assisted emulsion. The best conditions for a stable emulsion were 73.75% water, 25% carotenoid-rich oil extract, 1.25% emulsifiers, and 480 W of ultrasonic power for 5 min. The optimized emulsion had a total carotenoid content of 67.61 μg/g, Provitamin A activity of 3.23 ± 0.56 μg RAE/g, droplet size of 502.23 nm, polydispersity index of 0.170, and zeta potential of -32.26 mV. This emulsion was chemically and physically stable for 35 days at 30 ± 2 °C, showing potential as a food additive with biofunctional properties. The strategy here developed is an economical and environmentally friendly process that allows the reuse of the byproduct of B. gasipaes.

New Short-Circuited Coaxial Method Implementation for Complex Permittivity Measurement of Power Transformer Oils

The investigation into the dielectric properties of transformer oils has been a focal point in both historical and contemporary electric insulation technology as high-voltage applications have greatly benefited from the continuous research efforts in this field. The dielectric permittivity is one of the factors to be negotiated when discussing the electric insulation of any material. This work aims to showcase a new short-circuited coaxial cable method to evaluate the complex dielectric permittivity of palm, sunflower and rapeseed oil and review other previously used measurement techniques. Mainly, the test was performed using a sample holder that represents a short-ended coaxial cable filled with the test material which is connected to one port of a vector network analyser. The measurements of the input impedance for various oil samples were conducted at frequencies ranging from 1 MHz to 10 MHz. The technique uses the value of the input impedance which depends on the reflected signals from the test sample to the network analyser, consequently, the value of the dielectric permittivity and dielectric loss have been calculated using the short circuit impedance formula which involves numerical methods to get the results which shows that this method can be used as an alternative to investigate the oils insulation parameters as it provides smooth results for permittivity without any divergence, ensuring more reliable and consistent measurements as well as providing high accuracy in determining the permittivity of materials.

Investigation of synergistic effects incorporating esterified lignin and guar gum composite aerogel for sustained oil spill cleanup

The recently developed aerogel demonstrates a high capacity for pollutant absorption, making it an environmentally friendly option for oily water treatment. In an effort to reduce the adverse effects of the black liquor accumulation in the pulp industry, this study focused on utilizing the mentioned abundant bio-resource lignin, which can be applied to various high-value applications such as 3D porous materials for oil spill cleanup. Lignin, precipitated from the black liquor, was esterified using maleic anhydride as the esterifying reagent to enhance the hydrophobicity. Then, the composite aerogel fabricated from esterified lignin and guar gum (GG) was successfully prepared through the facile freeze-drying, using glutaraldehyde (GA) as the cross-linker. The resulting aerogel exhibited high porosity values exceeding 95%, low density (27.4 mg/cm3), and an impressive absorption capacity of 32.5 g/g for sunflower oil. These results demonstrate the potential of black liquor utilization as a bio-waste source of lignin and highlight the cost-effective guar gum-esterified lignin composite aerogel, which exhibits remarkable oil absorption capabilities and environmental sustainability promotion.

A modified sequential wavenumber selection-discriminant analysis with Bayesian optimization strategy for detection and identification of chia seed oil adulteration using Raman spectroscopy

The detection of oil fraud can be accomplished through the use of Raman spectroscopy, which is a potent analytical technique for identifying the adulteration of edible oils with inferior or less expensive oils. However, appropriate data reduction and classification methods are required to achieve high accuracy and reliability in the analysis of Raman spectra. In this study, data reduction algorithms such as principal component analysis (PCA) and modified sequential wavenumber selection (MSWS) were applied, along with discriminant analysis (DA) as a classifier for detecting oil fraud. The parameters of DA, such as the discriminant type, the amount of regularization, and the linear coefficient threshold, were optimized using Bayesian optimization. The methods were tested on a dataset of chia oil mixed with 5–40 % sunflower oil, which is a common form of fraud in the market. The results showed that MSWS-DA achieved 100 % classification accuracy, while PCA-DA achieved 91.3 % accuracy. Therefore, it was demonstrated that Raman spectroscopy combined with MSWS-DA and Bayesian optimization can effectively detect oil fraud with high accuracy and robustness.

Saffron (Crocus sativus L.) stigmas as a potential natural additive to improve oxidative stability attributes of sunflower (Helianthus annuus L.) oil stored under different conditions

The aim of our study was to explore the antioxidant potential of Moroccan saffron stigmas (SS) and examine their performance as a natural antioxidant in enhancing the stability and quality properties of edible sunflower oil (SO), with a focus on understanding the critical importance of edible oil stability for predicting quality deterioration during storage. Bearing this in mind, our research was designed to compare the efficacy of three concentrations of dried SS (at 0.2%, 0.3%, and 0.6%) against tocobiol (a synthetic antioxidant) as a positive control (at 0.3% concentration). This comparison was conducted under three distinct storage conditions namely accelerated ageing (60 °C), exposure to light at ambient temperature, and darkness, to evaluate their impact on preventing severe oxidation and extending oil shelf-life. Oxidation state evolution was evaluated through peroxide value (PV), free fatty acid (FFA), anisidine value (p-AV), K270 value (conjugated trienes), total oxidation index (TOTOX), iodine value (IV), and fatty acid composition (FA). Our results revealed notable differences in stability tracking parameters. Specifically, these parameters were higher in samples stored under accelerated conditions, followed by the samples stored in ambient light, while those stored in darkness showed the highest stability among the three storage conditions. Supplementation of sunflower oil with SS and tocobiol significantly enhanced its oxidation stability. Notably, SS exhibited exceptional effectiveness in stabilizing sunflower oil regardless of SS dose, with the highest efficacy observed at 0.6%. This was evidenced by the slower rate of oxidation parameters under various storage conditions, highlighting a superior antioxidant activity compared to both the non-enriched oil and tocobiol-enriched oil. Furthermore, saffron stigmas, used as a natural supplement, contributed to the preservation of polyunsaturated fatty acids, indicating its potential as a robust source of natural antioxidants in sunflower oil. These attributes position SS as a promising alternative to synthetic antioxidants, offering opportunities to enhance the nutritional quality and extend edible oil shelf-life.

Advances in the Use of Four Synthetic Antioxidants as Food Additives for Enhancing the Oxidative Stability of Refined Sunflower Oil (Helianthus annuus L.)

The present investigation was performed to evaluate the effects of various synthetic antioxidants (vitamin A, vitamin E, β-carotene, and BHT) on the oxidation of sunflower oil subjected to accelerated thermal storage at 60 °C for three months (12 weeks). The performance of the antioxidants studied was evaluated using several quality parameters: the free fatty acid value (FFA), primary oxidation (via the peroxide value (PV) and K232 value), secondary oxidation products (via the anisidine value (p-AV) and K270 value), and the total oxidation value (TOTOX). The fatty acid composition (FAC), oxidizability value (COX), iodine value (IV), and pigment content (chlorophyll and carotenoid) were also evaluated. The results revealed that the control sample of sunflower oil exhibited higher susceptibility to oxidative deterioration. Antioxidants at 200 ppm were more effective in preserving the oxidative stability of sunflower oil subjected to accelerated storage compared to the control oil. The smallest increases in all stability parameter indexes were recorded for antioxidant-supplemented sunflower oil. However, the IV and chlorophyll and carotenoid contents were reduced. At 200 ppm, vitamin E and β-carotene showed the greatest stability in sunflower oil, while their combination with vitamin A at 100 ppm of each showed the lowest stability. In addition, synthetic antioxidants provided greater protection against the degradation of polyunsaturated fatty acids (PUFAs). The highest level of PUFA degradation was recorded in the control oil, followed by the oil containing vitamin A. In conclusion, adding synthetic antioxidants to sunflower oil improves its stability during storage. However, some authors associated these molecules with a health risk due to carcinogenic effects as these molecules have been listed as “Generally Recognized As Safe” (GRAS).

1600-P: Sunflower Oil HFD Feeding in Mice Leads to Gut Dysbiosis, Mild Steatohepatitis, and Impaired Glycemic Control

1600-P: Sunflower Oil HFD Feeding in Mice Leads to Gut Dysbiosis, Mild Steatohepatitis, and Impaired Glycemic Control

Candelilla Wax and Glycerol Monostearate-Based Oleogels as Animal Fat Substitutes in Bologna Sausages.

The aim of this study was to produce Bologna sausages rich in unsaturated fatty acids and to evaluate this replacement on the structural characteristics. For the purpose of a comparative analysis, three different types of sausages were produced, distinct only in the type of fat used: I. sausages obtained with pork backfat (PBF), II. sausages produced with oleogel formed from refined sunflower oil and glycerol monostearate (GM_OG), and III. with candelilla wax oleogel (CW_OG). The meat composition was also analyzed to better understand the process in the dynamics and the finished products were analyzed both uncooked and cooked. The enhanced oil-binding capacity of oleogels suggests their potential value as substitutes for saturated fats (>99%). In terms of meat composition textural analysis, the highest hardness value was registered for PBF_C of 25.23 N, followed by a CW_OG_C of 13.08 N and a GM_OG_C of 12.27 N. However, adhesiveness, cohesiveness, springiness index, and gumminess showed similar values between samples. Reformulation of products with oleogels as a fat source abundant in mono- and polyunsaturated fatty acids resulted in uncooked products exhibiting reduced hardness values of 49.01 N (CW_OG_US) and 40.51 N (GM_OG_US), compared to 65.03 N (PBF_US). Color results of the cross-section color can indicate the potential for consumer acceptance due to the reduced color differences between the conventional and oleogel samples.

Liver phospholipid fatty acid composition in response to chronic high-fat diets

Liver phospholipid fatty acid composition depends on the dietary lipid intake and the efficiency of hepatic enzymatic activity. Our study aimed to simultaneously investigate the liver phospholipid fatty acid composition in response to chronic linseed, palm, or sunflower oil diets. We used adult female C57/BL6 mice and randomly divided them into control and three groups treated with 25 % dietary oils. Prior to treatment, we analyzed the fatty acid profiles in dietary oils and hepatocytes and, after 100 days, the fatty acid composition in the liver using gas-liquid chromatography. Linseed oil treatment elevated alpha-linolenic, eicosapentaenoic, and docosapentaenoic acids and reduced arachidonic and docosatetraenoic acids, consequently lowering the n-6/n-3 ratio. Palm oil treatment increased linoleic acid and decreased docosahexaenoic acid, contributing to an elevated n-6/n-3 ratio. Sunflower oil treatment elevated total monounsaturated fatty acids by increasing palmitoleic, oleic, and vaccenic acids. The estimated activity of Δ9 desaturase was significantly elevated in the sunflower oil group, while Δ5 desaturase was the highest, and Δ6 desaturase was the lowest after the linseed oil diet. Our findings demonstrate that chronic consumption of linseed, palm, or sunflower oil alters the distribution of liver phospholipid fatty acids differently. Sunflower oil diet elevated total monounsaturated fatty acids, proposing potential benefits for liver tissue health. Considering these outcomes, a substantial recommendation emerges to elevate linseed oil intake, recognized as the principal ALA source, thereby aiding in reducing the n-6/n-3 ratio. Moreover, modifying dietary habits to incorporate specific vegetable oils in daily consumption could substantially enhance overall health.

In-situ enzyme-initiated production of hexanal from sunflower oil and its release from double emulsion electrospun bio-active membranes

The aim of the presented study was to evaluate the release of the enzymatically initiated production of hexanal from double emulsion electrospun bio-active membranes at a temperature of fruit storage. Among different formulations of water-in-oil (W1/O) primary emulsions, the emulsion composed of 12% w/v Tween20 and 0.1 M NaCl in water (W1) and 6% of poly(glycerol) poly(ricinoleate) dissolved in sunflower oil (O) using W1/O ratio of 80/20 (w/w) (Tween20-NaCl/6% PGPR) was selected, for further incorporation of enzymes, based on the lowest average droplet size (391.0 ± 15.6 nm), low polydispersity index (0.255 ± 0.07), and good gravitational stability also after 14 days. Both enzymes, lipase and lipoxygenase are needed to produce hexanal (up to 58 mg/L). Additionally, double emulsions were prepared with sufficient conductivity and viscosity using different W1/O to W2 ratios for electrospinning. From the selected electrospun membrane, up to 4.5 mg/L of hexanal was released even after 92 days.

The effects of heating and acidulation on the properties and stability of plant seed protein emulsion gels

AbstractThis study aimed to prepare water-in-oil-type gelled protein emulsions. Sorghum, chickpea, and barley proteins were extracted from the seed flours with an alkaline procedure, and 2% protein solutions were used as an aqueous phase with Span 20 emulsifier to create sunflower oil emulsion gels. Prepared gels were analysed for main physicochemical properties, polymorphism, and rheology. Results showed that stable emulsion gels were creamy-greenish in colour and had mechanical stability. They included β and β′ type fat crystals. Rheological analyses indicated that they were soft-gel-like preparates, having certain levels of structural recovery ability after shear. These emulsion gels could be spreadable-fat-like products or shortening alternatives in processed foods. They might provide an extra advantage due to plant protein content. Food application studies are anticipated in future studies.

Antifungal and Antibiofilm Activities of Ozonated Sunflower Oil against Candida albicans Strains: A Pilot Study and Clinical Perspectives

Antifungal and Antibiofilm Activities of Ozonated Sunflower Oil against Candida albicans Strains: A Pilot Study and Clinical Perspectives

Biophysical studies of modified PVC sheet based on sunflower oil for antistatic and blood bags applications

In this work, the surface of polyvinyl chloride PVC sheet was modified by blending it with sunflower seed oil SSO to obtain PVC sheet/SSO films of ratios 100/0, 90/10, 80/20, 70/30, 60/40, and 50/50 (v/v)% using the solution casting method. Various techniques were used to characterize the prepared films, besides the use of hemolysis assays and blood clot formation tests. FTIR spectra revealed that there was a good interaction between the PVC sheet and the oil. The dielectric measurement indicated that SSO addition enhanced the dielectric properties of the sheet. The study of dielectric relaxation times confirmed the interaction between SSO and the sheet. DC conductivity increased to 6 × 10–6 S/m, so it could be applied in antistatic applications. Also, SSO addition increased the value of the thermal stability. According to SEM micrographs, the film was roughened at a ratio of 60/40 and smoothed out at 50/50. This behavior was confirmed with roughness and contact angle measurement results, in which the film of ratio 60/40 had the highest value equal to (72.03°) and then decreased at 50/50 to (59.62°). These results were confirmed by XRD measurement as the crystallinity increased at the film ratio of 60/40 and decreased again at 50/50. Also, the ratio of 60/40 demonstrated a large decrease in thrombus weights along with a slight increase in hemolysis, which is within the acceptable range and has a high degree of biocompatibility, so this concentration is recommended to be used in blood bags applications.

Investigating the nonlinear optical response of virgin cherry kernel oil and its application to detecting adulteration

The nonlinear optical (NLO) response of materials is the basis for their use in various applications including optical switching, optical data storage, and optical limiting. Additionally, it can be used as an innovative authentication method. In this study, the NLO properties of virgin cherry kernel oil (VCKO) were investigated by observing of spatial self-phase modulation (SSPM) diffraction rings using a 532nm continuous-wave laser beam. Based on the intensity-dependent number of symmetric diffraction rings, the nonlinear refraction coefficient and thermo-optical coefficient of VCKO were estimated to be 3.81×10−6cm2/W and 5.34×10−4K−1, respectively, for the first time, according to the authors’ knowledge. Furthermore, we employed the SSPM technique for adulteration detection in the VCKO sample by analyzing its NLO properties. To accomplish this, we prepared different adulterated samples by mixing VCKO with cheaper commercial oils, such as virgin sunflower oil (VSFO) and virgin canola oil (VCO) at various concentration ratios. In the mixed VCKO adulterated samples, the measured NLO parameters were highly correlated with the amount of adulteration. The results confirmed the ability of the SSPM technique to determine the degree of adulteration in oil samples by measuring and comparing their NLO properties. In addition, our results indicate that cherry kernel oil can be a viable candidate as a nonlinear medium for optical applications.

Energy Flow Analysis in Oilseed Sunflower Farms and Modeling with Artificial Neural Networks as Compared to Adaptive Neuro-Fuzzy Inference Systems (Case Study: Khoy County)

The evaluation of energy input and output processes in agricultural systems is a crucial method for assessing sustainability levels within these systems. In this research, the investigation focused on the input and output energies and related indices in sunflower farms in Khoy County during the agricultural year 2017–2018. Data were collected from 140 sunflower producers through specialized questionnaires and face-to-face interviews. Additionally, artificial neural networks (ANNs), specifically the multilayer perceptron, were employed to predict the output energy. The results revealed that a substantial portion of the total input energy was attributed to chemical nitrogen fertilizer (43.98%), consumable fuel (25.74%), and machinery (8.42%). The energy efficiency (energy ratio) in these agroecosystems was relatively low, measured at 1.57 for seed and 7.96 for seed and straw. These values should be improved. The energy efficiency in seed production was computed at 0.06 MJ·ha−1, while, for the combined seeds and straw, it was 0.57 MJ·ha−1. In particular, seed energy efficiency represents approximately 11% of the overall biological energy efficiency, highlighting that a substantial 89% of the produced energy is associated with straw. The proper use of this straw is crucial, as its improper handling could lead to a drastic decrease in overall efficiency. Furthermore, the explanatory coefficient (R2) and the mean absolute percentage error (MAPE) to predict the output energy with the best neural network were 0.94, and 1.77 for the training data, 0.97 and 1.55 for the test data, and 0.9 and 2.08 for the validation data, respectively; additionally, 0.97 and 0.42 were obtained by an ANFIS.

Analysis and authentication of avocado oil by low-field benchtop NMR spectroscopy and chemometrics.

Avocado oil is a nutritious, edible oil produced from avocado fruit. It has high commercial value and is increasing in popularity, thus powerful analytical methods are needed to ensure its quality and authenticity. Recent advancements in low-field (LF) NMR spectroscopy allow for collection of high-quality data despite the use of low magnetic fields produced by non-superconductive magnets. Combined with chemometrics, LF NMR opens new opportunities in food analysis using targeted and untargeted approaches. Here, it was used to determine poly-, mono-, and saturated fatty acids in avocado oil. Although direct signal integration of LF NMR spectra was able to determine certain classes of fatty acids, it had several challenges arising from signal overlapping. Thus, we used partial least square regression and developed models with good prediction performance for fatty acid composition, with residual prediction deviation ranging 3.46-5.53 and root mean squared error of prediction CV ranging 0.46-2.48. In addition, LF NMR, combined with unsupervised and supervised methods, enabled the differentiation of avocado oil from other oils, namely, olive oil, soybean oil, canola oil, high oleic (OL) safflower oil, and high OL sunflower oil. This study showed that LF NMR can be used as an efficient alternative for the compositional analysis and authentication of avocado oil. PRACTICAL APPLICATION: Here, we describe the application of LF-NMR for fatty acid analysis and avocado oil authentication. LF-NMR can be an efficient tool for targeted and untargeted analysis, thus becoming an attractive option for companies, regulatory agencies, and quality control laboratories. This tool is especially important for organizations and entities seeking economic, user-friendly, and sustainable analysis solutions.

Reduction of Fossil Fuel Usage and Emissions of Diesel Engines by the Application of Quaternary Blends and Varied Fuel Injection Pressures.

The increasing energy demand has led to the exhaustion of mineral fuel resources and an environmental menace. Biodiesel and alcohol, as oxygenated fuels, offer promising potential for diesel engines. Moreover, the deviation in the fuel injection pressure (IP) favors improvement of the engine performance and reduction of flue gases. The contemporary research aims to explore sustainable biofuel that is an alternative to diesel and to achieve cleaner emissions with enhanced engine performance. The experiment involves testing of a diesel engine tank by quaternary blends comprising diesel, sunflower biodiesel, sunflower oil, and alcohol in the volumetric ratio of 50:25:5:20. The IP was varied from 300, 400, 500, to 600 bar at different engine loads of 10 and 20 N m at 1800 rpm of shaft speed. The quality of the quaternary blend was varied by the inclusion of alcohol having different carbon-chain lengths, namely, ethanol, propanol, butanol, heptanol, and decanol. The effect of alcohol inclusion and variation in the IP led to minimal brake-specific fuel consumption and maximal brake thermal efficiency for blended fuel containing 20% propanol, which was 17.39% lower and 8.70% higher than diesel, respectively. The same composition of the fuel blend offered the lowest smoke and CO2 emissions, which were 92.85 and 27.9% lesser than diesel; moreover, 7.36% lower NO x emission than diesel was achieved.