Vitamin D deficiency is highly prevalent worldwide, even in locations where it was assumed that UV radiation was adequate to prevent vitamin D deficiency. The aims of this study were to measure vitamin D status in a convenience sample of overweight adults in Puerto Rico, which has plenty of sun exposure throughout the year, and relate it to vitamin D intake and sun exposure. Measurements included serum 25(OH)D, anthropometry, food frequency questionnaire and sun exposure questionnaire, obtaining a summary score ranging from 0 (no exposure, dark skin) to 38 (maximum exposure, light skin). Serum 25(OH)D was explained as a function of vitamin D intake, sun exposure, age, and % body fat using a multiple linear regression model. Complete data were obtained for 98 subjects (66% females), with mean age of 51.6±7.5 y, and BMI of 33.5±6.3 kg/m2. Mean serum 25(OH)D levels were 31.1±9.4 ng/ml. Vitamin D status was normal (>30 ng/ml) in 55%, insufficient (20–30 ng/ml) in 14% and deficient (<20 ng/ml) in 31% of the subjects. Mean vitamin D intake was 340±352 IU/d (58% were below the DRI) and sun exposure score was 21.5±6.7. The regression model explained only 7% of the variation of serum 25(OHD) levels, with only % body fat (p=0.07) and vitamin D intake (p=0.08) being marginally significant. In conclusion, in this group of overweight individuals, 45% had low vitamin D status, which was not explained by vitamin D intake or sun exposure.