Sumy National Agrarian University Research Articles

Features of the rhiospheric microbiota of medicinal plants

Medicinal plants are the source of biologically active compounds that are in constant demand for the pharmacological industry. Active production of plant secondary metabolites is possible only under optimal conditions of plant growth and development. The state of medicinal plants is controlled not only by genotype and environmental conditions but by the qualitative and quantitative composition of their microbiota as well. The study of the structure and function of the rhizospheric communities of medicinal plants is important for obtaining of high quality medicinal raw materials. Microorganisms are the constant companions of higher plants, which can be used as a medicinal raw material. The rhizosphere microbiota is highly specific, even between different varieties of the same plant species. Each plant species has a specific microbiome of the rhizosphere, depending on the existing soil community. The rhizosphere of medicinal plants is marked by a special highly specific microbiome due to the specificity of root exudates. Active cell secretion of the roots provides nutrient substrates with microorganisms that form strong associations both inside the root tissues and on the root surface as well as in the soil around the roots. The purpose of the research was to study the effect of medicinal plants of different systematic groups on the composition of the microbial communities of the rhizosphere. The experiments were conducted in 2018–2019 at the nursery medicinal plant plot of the Department of ecology and botany of Sumy National Agrarian University.
 Ecological-trophic groups of microorganisms associated with the roots of medicinal plants in the experiment were represented by ammonifying bacteria, nitrogen-fixing bacteria and bacterias that destroyed of plant residues (cellulose-destroying bacteria). In the analysis of the total number of microorganisms of the rhizosphere revealed differences in the quantitative and qualitative composition of microbiota, due to the specific features of a medicinal plant. Positive influence on the development of microflora in the area of the roots and individual ecological-trophic groups had Mentha longifolia (L)., and a negative effect was observed in plants of Bergenia crassifolia L. It has been established that the number of microorganisms and the diversity of ecological-trophic groups is due to the belonging of a medicinal plant to a particular taxon. The number of microorganisms and their diversity decreased in the direction of: Mentha longifolia – Lysimachia vulgaris – Aristolochia clematitis – Achillea submillefolium – Bergenia crassifolia.

Assessment of the quality and safety of carpets with mycotoxicosis

Assessment of the quality and safety of carpets with mycotoxicosis

Effect of fluoroquinolones on the male chicken reproductive organs

The using of antibiotics in poultry has some disadvantages. The problem of antibiotic resistance of microorganisms is recognized globally and is currently one of the strategic goals worldwide. Fluoroquinolones are an important and large group of synthetic antimicrobials that have great importance in the treatment of infectious-inflammatory poultry diseases of different etiologies. Fluoroquinolones have high bactericidal activity against a number of bacterial pathogens that infect poultry including E. coli, Hebsiell spp., Pseudomonas spp., Staphylococoeus spp. and Chlamydia spp. Enrofloxacin (ENR) is an antimicrobial agent belonging to the group of third-generation fluoroquinolones. ENR has been historically used as veterinary medicine for treatment of gastrointestinal and respiratory infections in several animal species, including poultrys cursing diseases caused by gram-positive and negative bacteria. Enrofloxacin presents 1,4-dihydro-1-cyclopropyl-7-(4-ethyl-1-piperazinyl)-6-fluoro-4-oxo-3-quinoli;3-quinoline carboxylic acid. A further synthesis allowed the reaching of active substances of cyclopropyl, as an antibacterial action to get further extension. A crystalline active substance with faint yellow color that was obtained to develop in high purity, is hardly soluble in water at pH 7, but as the molecule contains acidic and basic groups, it is easily dissolved at both alkaline and acid pH. The goal of the work. The purpose of our study was to investigate the sperm of male chicken after the use of enrofloxacin and to identify metabolites. Materials and methods of research. The researches were conducted in the conditions of vivarium clinics of the veterinary faculty and laboratories of the department of epizootology and parasitology of Sumy National Agrarian University. 80 male chickens were placed for research, which were kept in metal cages for 5–6 individuals. The use, care and transportation of birds for toxicological research were with all applicable animal welfare laws. All reasonable steps were taken to avoid or minimize discomfort, distress or pain of birds. Results of research and discussion. The differences between normal motility and defect sperm in control and enrofloxacin treated birds were not founded. Weight of testes, wattles and combs was not affected by drug. Ascorbic acid, total protein, testosterone and cholesterol concentration were similar in control and enrofloxacin treated groups. It is suggested that enrofloxacin at doses used were safe for male chicken. The administration of the drug “Enzin 10%” to the male chicken did not affect the concentration of ascorbic acid in the testicles, total protein and cholesterol. These indicators are actively involved in spermatogenesis. Thus, it can be concluded that the use of the drug “Enzin 10%” at therapeutic doses for 10 days had no negative effect on spermatogenesis of the male chicken.

Target language teaching by means of e-learning: a case study

The article reveals the implementation of innovative e-learning technologies in the process of mastering the discipline “Target language of the professional use” by students of agricultural universities. The presented e-learning training course is an innovative tool that provides study of educational material in the mode of independent work with a computer as well as regulates the interaction of students with a teacher. Such online collaboration contributes to the improvement of the efficiency of target language knowledge formation through the introduction of upgraded teaching educational materials; stimulates interest in the study of target languages using innovative information and communication technologies; responsiveness of the feedback and the possibility of direct consultation with the teacher in the educational environment of the Moodle platform. The analysis of the results of the experimental survey showed predominantly sufficient and high levels of knowledge of students from the experimental groups who were trained by means of the electronic course; in contrast to the low and initial levels of knowledge of students from the control groups. Particular attention is paid to the characteristics of the content, structure and tasks of the electronic training course on learning English, German, Ukrainian and Russian as target languages for distance learning students of Sumy National Agrarian University.

Determination of acute toxicity parameters of “Zoodizin” disinfectant

An important element in ensuring the epizootic well-being of the poultry industry is disinfection. Modern poultry farming requires a large number of effective disinfectants. It is known that the resistance of microorganisms to the effects of disinfectants is based on a genotypic mechanism. The nature of the formation of resistance to disinfectants and antiseptics is different than antibiotics. With regard to disinfectants, resistance is formed more slowly and the proportion of resistant strains in the population of microorganisms may not be high for a long time. This is due to different mechanisms of formation of resistance to antibiotics and disinfectants, in the first case – plasmid mechanism, in the second – chromosomal. However, increasing the resistance to the active substance in disinfectants can be widespread, so it is necessary to periodically rotate disinfectants. The goal of the work – to investigate the parameters of acute toxicity of the disinfectant biocide “Zodizin”. The studies were conducted in the laboratory of Veterinary Pharmacy and the Vivarium of Sumy National Agrarian University. The drug “Zodizine” contains: polyhexamethyleneguanidine hydrochloride – 21.0 %, alkylldimethylbenzylammonium chloride – 3.0 %. For toxicological examination of the disinfectant, healthy white male rats and white female rats weighing 200 ± 10 g 1.5 years of age were used. In the study of acute toxicity of animals observed daily, noted the general condition of the animals, features of their behavior. Studies have found that the toxic effect of the disinfectant “Zodizin” clinically manifested almost equally in both males and females. The average lethal dose for the rat female was 1000.0 ± 35.0 mg/kg body weight, males 1033.0 ± 34.3 mg/kg. Therefore, according to the classification of substances by toxicity, the drug by intragastric administration can be attributed to low-toxic substances. Observations on animals revealed that 1–3 hours after oral administration of the drug in a subtoxic dose in laboratory animals, shortness of breath and inhibition of the central nervous system were noted. Most of them died during the first day. Subsequent observations of the surviving animals indicated that their motor response was suppressed over the next 24–72 hours. Conclusions and prospects for further research: 1. It was found that the average lethal dose of the drug “Zodizin” with oral administration to rats-females was 1000.0 ± 35.0 mg/kg body weight, males – 1033.0 ± 34.3 mg/kg. 2. Experimental studies have proved that the disinfectant “Zodizin” according to GOST 12.1.007-76, belongs to the IV class of danger, that is, to the low-dangerous compounds, and according to GOST 12.1.07 – to the III class of hazard of substances and can be used for disinfection premises where animals and poultry are kept. Further, the sporoсide and corrosion properties of the “Zoodizin” biocide will be studied.

The research of disinfective properties of kontravir for disinfection of veterinary objects

In this field, complex disinfectants were contrasted using effective concentrations against bacteria and spore-forming microorganisms. A significant problem for the owners is the emergence of resistance of strains of microorganisms in the existing production and the same disinfectants. Investigation of trusted working concentrations of disinfectants that do not have a corrosive effect on metal structures. According to the results of research and production, contrast is an effective disinfectant for reducing E. coli and S aureus at 0,3 – 0,5% concentration at exposure for 30 min. and consumption of 100 - 400 cm3 / m2.
 Laboratory researches were carried out in laboratories of microbiological faculties of veterinary medicine of Sumy National Agrarian University. Disappearance gaps and disinfection on the market in Kyiv. Samples were drawn in the refrigerators from the walls, ceilings and floors. Refrigerated chambers made of stainless steel are made up of rubber and plastic elements. Metals are very vulnerable to corrosion with more concentrated acids and alkali. This was taken into account when choosing a disinfectant and its effective organizations. As a disinfectant used the drug contrast (manufacturer PE "Kronos Agro", Ukraine). The disinfectant was taken at a dose of 100 ml per 1 m2. To produce the culture was prepared basic products containing 1000 mg of the drug in 1 ml of distilled water. The experimental solutions were prepared for study with the main formulations developed. Representatives had extraordinary effects. The disks were leaked through the disinfectant to obtain a zone of retention of high bacteria and fungi.
 The reliability of disinfectant destroying the micro-organisms of E. coli and S. aureus test cultures was also known. For the trusted production preparations, the contrast at the trusted enterprises was carried out by the rehabilitation of the premises of the educational laboratory of the Sumy National Agrarian University (premises for animals, dairies, refrigeration chambers).
 Renovation and test activities were performed at 0,1, 0,25 and 0,5 % of exposed contrast with exposure for 60, 30 and 10 min. the disinfectant was consumed 250 cm3 / m2. Upon completion of the studies, the investigated tests were investigated with distilled water (flow rate - 1000 cm3 / m2). Water that has been washed away test products, presented in specially prepared capabilities. Test results for this after disinfection were also investigated with distilled water, which was found on the disinfectant. To study the corrosion activity of the disinfectant used metal plates 1h1sm2. The metal samples were welded to the fifth mark after the commission before and after the study. 0,5; 1,0; 1,5; 2,5 % contrast solutions were used in the study. For comparison, in the experiments used 2% of productive. M. bovis mycobacterial cultures were grown on Pavlovsky's glycerol medium. The bacterial culture was transferred into sterile vials and sterile isotonic material containing 0,05 cm3 / mg was added. A large amount of delay zone in Petri dishes containing 0,5 % of S. aureus disinfectant, 3,5 times S. cholerase 1,8 S. Enteritidis 2 more than 0,5% formal form . Higher indicators of bacterial properties of the disinfectant contrast at a concentration of 0,5%.

Determination of quality of poultry meat in case of damage to ectoparasites

Introduction. Obtaining high-quality and safe poultry products is impossible without an integrated approach and competent solution of biosafety issues in farms. In conditions of poultry rearing using intensive technologies, the problem of ectoparasitic poultry damage, in particular the chicken tick Dermanyssus gallinae, is quite acute. The defeat of the bird by temporary and permanent ectoparasites is the reason for the deterioration of the quality characteristics of poultry carcasses.
 The goal of the work. The purpose of our research was to determination of the quality of poultry meat when affected by ectoparasites.
 Materials and methods of research. These studies were conducted under the conditions of the Department of Veterinary Expertise, Microbiology, Zohygiene and Safety and Quality of Livestock Products of Sumy National Agrarian University. For the study, carcasses of poultry of slaughter conditions were selected from a farm unsuccessful for the red chicken tick Dermanyssus gallinae.
 Results of research and discussion. In the course of the studies, it was proved that the slaughter yield of meat in chickens that were infected with ectoparasites was less compared to the slaughter yield of poultry meat obtained from farms or poultry houses that are relatively ectoparasite. In the meat of sick poultry, the moisture content increased by 2-3% and protein by 1-2%, while at the same time reducing the amount of fat by an average of 4%. As a result of studies, it was found that the changes found in meat indicated the development of pathological processes in meat, which led to the intensification of spoilage processes. Studies of carcasses obtained from healthy poultry found that they retained freshness for 9 days, and carcasses received from sick poultry, already for 5 days did not match the freshness of meat.
 Conclusions and prospects for further research. Based on a set of studies, we found that the qualitative indicators of meat of healthy and sick poultry differed. Intravital damage to poultry by ectoparasites caused changes in the chemical composition of meat, a decrease in its calorie content and biological value.
 In the future, it is planned to determine the qualitative characteristics of poultry carcasses in the associated course of nematodes and aсarosis.

Assessment of commodities fishing in the trade network of the city of Sumy

Introduction. To date, one of the priority tasks of the agro-industrial complex of Ukraine is to provide the population with benign and safe environmental and veterinary-sanitary products of animal products. Aquaculture is one of the fastest growing industries in the rapidly developing agro-industrial complex. The mentioned industry is able to provide the population with high quality nutritious and dietary fishery products in a relatively short period. Fish products replenish the human diet with essential amino acids, polyunsaturated fatty acids, trace elements and other nutrients to meet the needs of the body. But important indicators are the quality and safety of fish and fish products. The use of hazardous fish products by humans can lead to human diseases. Therefore, research on determining the safety and quality of fish remains an important link.
 The goal of the work. The purpose of our research was to investigate commercial fish sold in the Sumy trade network and to carry out its veterinary and sanitary evaluation.
 Materials and methods of research. These studies were conducted under the conditions of the Department of Veterinary Expertise, Microbiology, Zohygiene and Safety and Quality of Livestock Products of Sumy National Agrarian University and in Sumy Regional State Laboratory of the State Consumer Service.
 Conducted research on commercial fish, which came to the sale in the trade network of the city of Sumy, namely in supermarkets, specialized stores and agri-food markets. Fresh, dried and frozen fish were studied.. 
 Results of research and discussion. As a result of the study of the selected samples was found in live fish, namely the fathead, characteristic signs of postodiplastomosis. This disease manifested itself as small black spots on the body of a fish. It was also revealed in the study of carp in two specimens that the chronic course of aeromonosis was manifested by the presence of fissured ulcers on the body of the fish. Since the detection of the disease did not spoil the product indicators of fish, then all living fish are allowed to sell.
 When examining dried fish, no deviations from the requirements for dried fish were found in the submitted samples. All fish are identified as benign and admitted for sale.
 Studies of fresh frozen fish (hake) revealed the presence in the muscle tissue of the nematode Contracoecum squalii
 Affected fish are characterized by an increased content of microorganisms in the deep and superficial muscle layers, a doubtful or negative reaction to peroxidase, a doubtful or substandard reaction with a Nesler reagent, a doubtful or substandard reaction with sulfuric acid, acidification.
 Conclusions and prospects for further research: 1. In a study of 323 samples of live, dried, frozen fish, which entered the trade network in Sumy, it was found that 8 (2.47%) samples did not correspond to good-quality fish.2. Signs of diseases characteristic of aeromonosis (2 specimens), postodiplastomosis (1 specimen) and contracenosis (5 specimens) were established in the selected fish samples.3. A batch of hake (170 kg) in which the pathogen Contracoecum squalii was detected is not allowed for sale and is intended for technical disposal.
 In the future, it is planned to develop and implement a permanent monitoring system for the quality and safety of fish and fishery products that are commercially available.

Sanitary evaluation of fishes fished from the ponds of the Sumy region

Introduction. Fisheries are a traditional industry in Ukraine, started more than 300 years ago. There are more than 1 million hectares of fisheries in the country, including about 200,000 hectares of ponds. The main breeding sites are various carp breeds, white and mottled silver carp, white cupid. Fish and fishery products are important in human nutrition and make up a large part of their diet. In many countries of the world, fish is the main focus of the food industry.
 The goal of the work. The purpose of our research was to carry out a sanitary evaluation of fish caught from the sums of Sumy region.
 Materials and methods of research. These studies were conducted under the conditions of the Department of Veterinary Expertise, Microbiology, Zohygiene and Safety and Quality of Livestock Products of Sumy National Agrarian University and in the fisheries of Sumy region.
 Selection and preparation of fish samples for research were carried out according to GOST 7631-85. For the study used fish from the carp family, which are most common in the fishery farms of Sumy region.
 Results of research and discussion. When conducting organoleptic studies in sample No. 1, the following indicators were found: mouth closed; eyes clear, pale, convex with transparent cornea; cheeks and jaws pale; gill caps tightly against gills; gills with viscous, clear and transparent mucus, bright red, with a slight odor of fresh fish moisture, mucus in a small amount, viscous and transparent, odorless or with a slight odor of dampness; scaly shiny, clean; fins of lifelong appearance and color, without damage, the back is dense, elastic, the fossa from the pressure of the finger quickly, almost immediately disappears; the anal opening is closed, which corresponds to the indicators of good-quality fish; and in sample No. 2, the mouth is open; eyes sunken, dull, pale pink or pale red, with dull cornea; cheeks and jaws pale pink; gill caps do not fit snugly, move away from gills; on the gills dull mucus, mucus much; scales dim; fins covered with thick turbid mucus, at the base of the fins mucus pink or reddish; the back is soft, the fossa from the pressure of the finger slowly disappears; the anal opening is slightly swollen, pink-red, which indicates the quality of fish of dubious freshness. Meat toxicity determination of all fish species studied was performed using Tetrachymena pyriformis infusions. The absence of inhibitory effect on the survival of the infusions, their growth and behavioral response, the degree of mobility, morphological parameters. This testified to the absence of toxicity of all tested fish meat samples. After 3 hours the infusions were alive and mobile. Also, by determining the level of histamine, we found that it was within normal limits. Thus, the histamine content in sample No. 1 was 20,5 ± 3,1 and in sample No. 2, respectively, 22,4 ± 5,5 mg/kg.
 Conclusions and prospects for further research: 1. In the study of fish samples caught from the sums of Sumy region it was found that by organoleptic and physicochemical indicators, sample No. 1 corresponded to good-quality fresh fish, and by carrying out the study of sample No. 2, the results obtained were characteristic of fish of dubious freshness.
 
 The presence of live helminth larvae in humans and animals was not detected in all of the samples of chilled fish we selected for veterinary examination.
 Microscopy of specimens from specimens No. 1 revealed no microflora; drugs were stained poorly, which is characteristic of fresh fish, and in the preparation of sample No. 2 from the surface layers in one of the fields of view revealed 30-35 diplococci.
 
 In the future it is planned to carry out monitoring of the veterinary and sanitary evaluation of fish caught from the Sumy basin.

Breeding evaluation of chinese bread winter wheat varieties recent in the northeastern Forest-Steppe of Ukraine

Experimental researches had been carried out during 2012–2016 in the crop rotation of Sumy National Agrarian University (SNAU) of the Ministry of Education and Science of Ukraine. Soils on the experimental field of SNAU – black soil typical deep, non-humusful medium-loam with high and medium provision with elements of mineral nutrition. The humus content about 3.9 %. The reaction of the soil solution is close to neutral (5.8).
 The analysis of the weather conditions of 2012–2016 researches was conducted on the basis of annual data provided by the meteorological station of the Institute of Agriculture of the North-East of the NAAS, located five kilometers from the experimental field of SNAU. The SNAU soils are classified in the second agro-climatic region of the Sumy region, which according to a long-term data is characterized by temperate continental climate with warm summers and not very cold winters with thaws. Average daily (average annual) air temperature during 2012–2016 fluctuated from +7.9 to +9.5 ºС, and the legth of the frost-free period was close to 230 days. Long-term indicator, precipitation falls within 597–600 mm, with most of it – in the warm period (April-October). In general, the weather conditions during the winter wheat vegetation periods differed from the average annual parameters of the temperature regime, the amount of precipitation and their monthly distribution. It should be noted the excess of temperature to the average long-term index, as well as a slight precipitation increasing. In general, it facilitated to a comprehensive evaluation of the studied Chinese varieties as for an adaptive ability under condition of Ukraine.
 The samples of Chinese winter wheat varieties which originated from the expeditionary gatherings conducted by V. A. Vlasenko in Gansu and Hebei provinces in (2000–2012) were the material for conducting researches. The cultivar Podolianka (the standard) was used in the study for comparison. The research was carried out using field, laboratory and mathematical-statistical methods. Phenological observations and records, evaluation and harvesting were conducted in accordance with generally accepted methods.
 The results of research as for adaptive potential of Chinese bread winter wheat varieties are presented. 50 new cultivars of Chinese origin were analyzed under the conditions of left-bank side of North-East Forest-Steppe of Ukraine: super-early varieties – 10 %; early ripening varieties – 54 %; middle-early varieties – 12 %; mid-ripening varieties – 24 %. As for the level of tolerance for winter conditions, all groups of alien crops were inferior to the standard (cultivar Podolyanka) though they had a great level of index (6.4–7.9 points). In general, Chinese cultivars under the conditions of the research were characterized by relatively satisfactory tolerance for winter conditions; 52 % of analyzed patterns wintered at the level of standard with 8 points. Among them: super-early varieties – 2 %; early ripening varieties – 20 %; middle-early varieties – 8 %; mid-ripening varieties – 22 %. As for the height of the plants we identified – from medium-sized (81‒110 cm) forms to dwarf (30-50 cm). The analyzed genotypes divided into medium-sized forms – 22 %, dwarf forms – 14 % and semidwarf forms – 64 %. There was a direct relation between: a plant height → group of ripeness (r = 0.96) → group of ripeness (r = 0.92) → resistance to overwintering (r = 0.78). Among the analyzed patterns high resistance against a group of diseases had the varieties: middle-early genotypes – Longzhong 10, Zhong mai 19, Shijra zhuang 8; mid-ripening genotypes – Longzhong 1, Longzhong 3, Longzhong 2, Longzhong 4, Longzhong 12, Longzhong 7; early ripening genotypes – DF529, Lankao 906, DF581, СА0175, Zhongmai 9, Shi 4185, Jimai 22, Shixin 733, Shimai 12. As for the crop better than the standard: early ripening varieties –Jimai 19, DF529; middle-early varieties – Shijiazhuang 8, Longzhong 10, Jingdong 8; middle-early varieties – NSA 97-2082, Longzhong 2, Longzhong 4, Longzhong 7, Longzhong 5, Longzhong 8, Longzhong 12, Longzhong 11, Longzhong 3. 16 % of analyzed cultivars distinguished by the totality of researched characteristics – DF529, Shijiazhuang 8, Longzhong 3, Longzhong 10, Longzhong 4, Longzhong 7, Longzhong 12, Longzhong 2.

Integrated population analysis as a direction of the modern biological and ecological researches

Some historical aspects of the formation of population ecology and biology as a separate scientific field are highlighted. It is noted that at the present stage, an integrated approach of studying populations is increasingly used. The work provides information on the main components of complex population studies implemented at the scientific school of plant population ecology created at Sumy National Agrarian University by Prof. Yu.A. Zlobin. The main achievements of the representatives of this school are shown, and it is also noted that, on the basis of their scientific achievements, the number of studied objects is gradually expanding. An example of this is the use of integrated population analysis for the study of protected autochthonous dendrosozophytes of Ukrainian Polissia whose representative, in particular, is Oxycoccus palustris Pers. Using the example of this species, the basic aspects of the application of complex population analysis are described, including determining the size of the population field, population density, size and morphological characteristics of individuals that form the populations, as well as the ontogenetic, size, and vital structure of populations. Based on such studies, swamp and forest-swamp phytocenoses were differentiated according to the degree of favorableness for the existence and functioning of O. palustris populations. At the same time, populations that can be considered as objects of regulated procurement of plant materials of O. palustris were identified. The conditions of the oligotrophic swamp and the grouping of Sphagnetum (cuspidati) eriophorosum (vaginati) are proved to be the most favorable for the formation of Oxycoccus palustris coenopopulations with high population density, vitality, diversity of size structure and balanced structure Populations from this grouping can be considered as centers of regulated harvesting of plant products and plant materials of the investigated species. Compared to swamp, forest-swamp phytocoenoses were less favorable for the formation and existence of coenopopulations of O. palustris, although they also revealed coenopopulations with a complex of high population characteristics Based on the results of studies and elucidation of the characteristics of populations, the original coenopopulation principle of constructing phytocenocompositions from species of natural flora was also proposed and tested.
 Its essence is that in the selection of certain species in the composition of phytocenocompositions it is necessary to rely on the indicators of phytocenoses, in which its populations have signs of ability for stable and long-term existence in the composition of plant groups The determinants features in this complex are the area of the population field, population density and characteristics of the structure, especially ontogenetic and vitality. According to the first feature of these traits, it is necessary that the populations belong to the group of young, transient or maturing and not to be aging or old, and according to the second – to represent the category of prosperous.

Efficiency of bacterial fertilizers in peas agrocoenosis under the conditions of the northeastern Forest-Steppe of Ukraine

Peas (Pisum sativum L.) is an appreciable annual leguminous crop which can solve the problem of phytoalbumin, improve nitrogen balance of soil and increase the production of food products.
 The important role in cultivation method of leguminous crops, pea in particular, plays the realization of productive potential due to energy-saving techniques, namely seed inoculation. One of the ways to supply pea agrocoenosis with beneficial microflora is the use of bacterial fertilizers which can supply the plants with essential complex of microorganisms, good nutrition and as a result it can help to realize its genetic potential as for yielding capacity and protein content.
 The aim of the research is to define the influence of bacterial fertilizers on pea productivity by different level of mineral nutrition. The researches have been conducted on the base of educational and scientific centre of Sumy National Agrarian University according to generally accepted methods during 2015–2017.
 The research variants were the following: without inoculation by bacterial fertilizer and with seed treatment with Rhyzohumin and Polymyxobakteryn. Inoculation of peas seed was conducted according to the method of the Institute of Agricultural Microbiology and Agro-Industrial Production of NAAS (Chernihiv).
 On the control the seed inoculation has not been done. The backgrounds of mineral nutrition were P60K60 and N60P60K60. The application of bacterial fertilizers which contain new, highly effective, culturally specific variety of rhizobial bacteria with the increased viability in high concentrations ensures the development of maximal amount of tubers on root system of plants. The highest efficiency in forming symbiotic apparatus and intensity of its activity was stipulated by combination of presowing seed inoculation with Rhezohumin and application of phosphate-potassium fertilizers in the doze of P60K60. The maximal amount and weight of nodules was 50.3 unit per plant and 2.06 g per plant. The level of peas yielding capacity is defined by individual plant productivity which is multiplied by its amount and characterizes the cro productivity under the certain conditions of growing.
 It was determined that the combination of seed inoculation by bacterial fertilizers through using of nitrogen fixing (Rhizobium leguminosarum strain 31) and phosphorus mobilizing (Bacillus polymyxa KB) bacteria and mineral fertilizers favours to raising of peas productivity under the conditions North-East Forest-Steppe zone of Ukraine. The most effective result was obtained in the variant with peas seed inoculation by Rhyzohumin and application of mineral fertilizer in the doze N60P60K60 (yield has increased on 33.7 % comparing with the control).

Development of the leaf area and the productivity structure of the sunflower hybrids in the north-eastern Forest-Steppe of Ukraine

At the present stage, the model of leaf plant development is increasingly considered as the main element of genotypes adaptation to specific cultivation conditions, geographical area, etc. Under these conditions, the potential of the hybrid, the range of its adaptation to environmental conditions is determined by the level of interaction between the development of the assimilation apparatus and the generative organs. An urgent task aimed at improving the productivity of sunflower crop due to the use of genotypes adapted to the conditions of the north-eastern Forest-Steppe and Polyssya of Ukraine is to identify typical schemes of interaction of photosynthetic potential and parameters of plant productivity and crop yields.
 The studies were conducted in accordance with the program for the development of a variety model for the conditions of the north-eastern Forest-Steppe and Polyssya of Ukraine. Field experiments were carried out in 2016‒2019 at the Sumy National Agrarian University and the Institute of the Agriculture of North East of Ukraine. In the studies, 28‒56 hybrids of different originators were tested annually. Sunflower was cultivated according to the technology recommended for the area, with pre-harvesting density of 60 thousand plants / ha. Harvesting was done manually, from two central rows of a 4-rows plot. The results were processed using the Statistics package. 
 The data on yield indices and values of leaf area coefficient (LAC) of crop for groups of varieties separated by the duration of growing season were analyzed. It was found that the increasing of leaf area was accompanied by an increasing of yield in case of comparing groups with dates of technological maturation until 20 August, 1 September and 10 September. In all cases, the maximum value of the leaf area index ranged from 3.12‒3.52 m2/m2. These dynamics of indicators indicated to the regulatory nature of the values of leaf area coefficient (LAC) of modern sunflower crop and the absence of genotypes (or conditions) capable of maintaining these values at the level of more than 3.3‒3.5 m2/m2.
 According to the analysis of correlation pleiades, the presence of several levels was found and the relative independence of the relationships between the group of parameters, determining the morphological structure of plants and the vertical structure of crop and the group of parameters, characterizing the content of chlorophyll and its concentration per unit area of leaf surface. In practical terms, the results of the analysis indicated the potential informative nature of the complex use of parameters characterizing the morpho-structure of plants, primarily the index of leaf area and the index of chlorophyll concentration per unit area.
 In order to isolate the typical schemes characteristic of different levels of adaptation to the conditions of the zone, data on 29 sunflower hybrids distributed in the region were clustered. The results of the analysis allowed to distinguish three significantly different algorithms for the realization of the vegetative and generative potential of sunflower hybrids in the area of the north-eastern Forest-Steppe of Ukraine. It has been established that one of the factors for successful realization of the generative potential of sunflower hybrids in the conditions of the zone is the ability to preserve and (in some cases) to improve the structure of relations between the dynamics and parameters of the plant leaf apparatus and the parameters of their generative development.

Influence of external conditions on the manifestation of the average number of tubers in the bush among interspecifics hybrids of potatoes, their backcrosses

The ability to form a large number of tubers in the nest positively affects the productivity of hybrids and potato varieties. However, the expression of the trait is subject to a significant influence of external conditions, depending on the rate of response of the genotype. Studies on the assessment of the varying complexity of interspecific hybrids, their backcrosses in the ability to tie tubers were carried out during 2015‒2017 in three places: the experimental field of the Sumy National Agrarian University (hereinafter SNAU), the Ustimov Experimental Plant of the V. Y. Yuryev Institute of Plant Production of the NAAS of Ukraine (UES ), as well as the Institute of Potato Production of the NAAS of Ukraine (IR). The source material used 26 complex interspecific hybrids, their backcrosses. Varieties standards was Yavir and Grouse. The technique is generally accepted in the study with potatoes. The locations and years of the experiment varied significantly in meteorological conditions. SNAU alone had more rain in 2016 than in many years. In the UES, this applied to 2015 and 2016, and in the IR all the years there was a significant deficit in the supply of moisture with rains. Under the conditions of SNAU, only three decades from 12 in 2015, the air temperature was below the long-term average. In subsequent years there were five. In UES, this, respectively, amounted to 5, 7 and 7 decades. In IK, in all months, the air temperature was above the long-term average; in May 2015 and August 2015 and 2017, the difference exceeded 50 °C.
 The high potential of the studied samples was determined by the ability to tie tubers. In some hybrids, under optimal conditions, their average number in the nest exceeded 20 pcs. A particularly positive effect on the formation of tubers was revealed in 2016 in the IR, when some of them with the maximum manifestation of the trait amounted to 32.1 %. To a lesser extent, the foregoing related to a part of the material with the number of tubers in the nest of 10 pieces and more ‒ 8.4 %. In all places throughout all years hybrids with the best tuber-forming ability were distinguished than the variety-standard Teterev. In general, some of these hybrids amounted to 0.4‒7.7 %.
 The distribution data of the manifestation of the trait among hybrids depending on the place and years of testing indicate that in 2015 and 2016 with a tuber/nest of 8.1–10.0 tubers/nest, and 6.1–8.0 in the latter, the modal class in SNAU. Very unfavorable conditions for tying tubers were identified in 2016 under the conditions of UES. More than half of the test material is assigned to class 4 tuber/nest and less. In the other two years, the modal class was 6.1‒8.0 tuber/nest. In all years, under conditions of infrared hybrids there was no hybrids with a minimum value of the indicator, and the modal classes were, respectively, 8.1‒10.0; more than 12.0 and 6.1‒8, as well as 8.1‒10.0.

Pear rust in the conditions of north-eastern Forest-Steppe of Ukraine

Studies were conducted during 2017‒2019 based on the training laboratory of horticulture and viticulture of the Sumy National Agrarian University in the conditions of the North-Eastern Forest-Steppe of Ukraine. It was found that the pear was massively affected by rust. This is a fungal disease that was rarely found in fruit orchards of the North-Eastern Forest-Steppe of Ukraine.
 Rust infected pear trees very rarely in previous years. Only single spots were found on the leaves of the pear. Since 2015, the development of the disease has noticeably accelerated from year to year. There was a massive rust damage of the pear in the region in 2019. The prevalence of the disease reached 100 % in almost all varieties. Mostly the leaves were affected, not to a large extent the shoots of the pear. On the fruits of the external signs of the disease were not detected.
 During the years of research, weather conditions were optimal for the spread of fungal diseases. Only the aecial stage of the fungus was observed on the pear. The aecial stage of the pathogen is the most harmful. Affected pear leaves fall prematurely. Studies were conducted on pear varieties Lymonka, Petrovska, Medova, Osinnia Yakovlieva, Chyzhovska, Noiabrska, Bere Desiatova, Uliublenytsia Klappa, which showed different degrees of rust damage. The disease manifested itself most significantly (5 points) on the varieties, Uliublenytsia Klappa and Bere Desiatova. Not one of the varieties did not show high resistance to the pathogen. The pear of the Chyzhovska variety was less affected, with a defeat score of 3 (18.8 %) in 2017 and 4 (32.3 % and 44.1 %) in 2018‒2019.
 It is known that the life cycle of the rust pathogen Gymnosporangium sabinae (Dicks.) G. Winter occurs on two plants: pear and juniper. The pathogen from juniper goes on the pear and vice versa. Pear trees do not become infected from each other. On the territory adjacent to the fruit garden of Sumy National Agrarian University, a survey of plantings of different types of juniper was conducted. There were no visible signs of the disease on the juniper. At the same time, pear trees were highly infected with the pathogen. Perhaps the spores of the fungus can spread far through air currents. But at the same time, possible changes in the life cycle of the pathogen in the conditions of this region. Clarification of this circumstance requires deeper further research.

Effects of testing conditions of complex interspecies potato hybrids, their backrosses on the average weight of commercial tubers

Purpose and objectives. To determine the potential of complex interspecies potato hybrids, their backcrosses in terms of the average weight of commercial tubers and to identify the effects of exrenal conditions on the trait expression.Materials and methods. The study was conducted in two soil-climatic zones: the northeastern forest-steppe of Ukraine (Sumy National Agrarian University) and southern woodlands of Ukraine (Institute of Potato Growing) in 2015–2017. We studied the influence of external conditions on the average weight of commercial tubers in 34 complex interspecies hybrids and their backcrosses. The technique was common in experiments on potato.Result and discussion. A high potential of the material under investigation was recorded for the average weight of commercial tubers with the maximum expression of the indicator in a double backcross of four-species hybrid 00.72 /5 in SNAU in 2017 –217 g.We experimentally proved the influence of the external conditions: the study locations and meteorological conditions during the growing period on the trait expression. Hybrids with the average weight of commercial tubers of >100 gin the SNAU conditions were the most frequent in 2017 (23.5%) and the least frequent in 2015 and 2016 (4.9% and 3.9%, respectively). In the IPG, there were no such hybrids in 2015 or 2017; their percentage only was 1.0% in 2016.The high potential of the material under investigation in terms of the trait expression is confirmed by occurrence of hybrids with an increased level of the indicator than that in the best standard variety, which was observed regardless of the external conditions.The best conditions for the formation of commercial tubers with the maximum weight were in SNAU in 2017; their percentage was 27.5% of their total number. Close, albeit lower, percentages were achieved in the IPG in 2016 and 2017: 15.7 and 13.7%, respectively.We observed significant effects impact of the external conditions during the potato growing period on the indicator expression. In SNAU trials, most of the hybrids had the variation coefficients within 21-30% and 31-40%, and in the IPG conditions they were £ 10% or 11-20%. The above-said was also confirmed by categorization of the hybrids, depending on the experiment location and the conditions during the growing period. In SNAU, 2017 was the most favorable for the trait expression. The opposite was true for2015. Inthe IPG, the best distribution of the material by the high average weight of one commercial tuber was noted in 2016 and the worst - in 2015.We revealed different relationships between the genetic control of the trait and the external conditions in sibling forms, which was manifested as various expression of the indicator depending on the trial location and the meteorological conditions in the trial years.Conclusions. The influence of the conditions in the study location and the meteorological conditions during the growing period on the expression of the average weight of commercial tubers was demonstrated. They affected the fulfillment of the genetic potential of the accessions under investigation, its maximum manifestation, the variation coefficients, and categorization of the hybrids into classes of the indicator values.

Decreasing of the risk of zoonoses by solving the problem with stray dogs

People can become infected with zoonoses from domestic or stray dogs (the most dangerous ones are rabies, brucellosis, dermatomycosis, leptospirosis, parasitic diseases). 1,466 rabies outbreaks and 2 cases of human rabies were reported in Ukraine in 2018. In total, 17 people died of rabies in Ukraine in 2014-2018. Sources of rabies were nonvaccinated against rabies dogs.
 In most dermatomycosis patients, the zoonotic form of microsporia caused by Microsporum canis is recorded, and infected cats and dogs play an important role in the spread.
 Dogs are a dangerous reservoir of leptospira and a source of infection for humans. Develops a mild course of leptospirosis, reminiscent of influenza, but has severe consequences and leads to meningitis.
 Dogs can infect humans with the agents of campylobacteriosis and brucellosis, as well as many other parasitic diseases: Ancylostoma braziliense і Ancylostoma caninum, Toxocara canis і Toxocara cati , Cryptosporidium , Echinococcus granulosus, Dirofilaria immitis , Giardia lamblia and ectoparasites: Sarcoptes scabiei (variant canis) and Cheyletiella yasguri. 
 Prevention of zoonotic diseases requires a holistic approach within the framework of the One Health concept. Health, 
 environmental and veterinary services, with the active involvement of local government, conservation and animal welfare organizations, must be involved.
 The problem of handling animals and regulating the numbers of stray animals in Sumy is an urgent one. The solution to this problem is to reduce the number of stray animals with only humane methods, to improve the epizootic and sanitary-ecological situation in the city, to create conditions for the prevention of the phenomenon of stray animals, and to change public opinion towards a civilized, humane, ethical attitude to animals.
 Principles of animal shelter organization, scheme of veterinary and sanitary measures and treatment and preventive treatment of animals have been developed and put into practice by us.
 Within the framework of the agreement with the communal enterprise of the Sumy City Council "Animal Care Center" Sumy National Agrarian University performs the veterinary part of the service of stray animals, which ensures the implementation of the "Program of regulation of the number of stray animals in the city of Sumy by humane methods".

The number of tubers in the bush in interspecific of the potatoes, their beckcrosses depending on the conditions of growth

The experiments proved the value of many complex interspecific hybrids, their backcrosses according to the ability to tie more tubers in the nest than the best standard variety. Under the favorable external conditions, it was measured at more than 20 pieces/nest. In most hybrids, a significant impact on the implementation of the indicator of the places for the research (Sumy National Agrarian University and Ustymiv Research Station) and meteorological conditions of the years of the experiment (2015-2017). Based on the data on the distribution of hybrids by the quantity of the tubers in the nest, it has been found out that the best conditions for the manifestation of the signs were in SNAU in 2015 and 2016, and in the Ustymov research station in 2015. The biggest number of hybrids (6 pcs.) with the difference of 7 tubers/nest depending on the places of the experiment were found out in 2017 and the lowest number – in 2015. The opposite was true for the minimum difference in the manifestation of the indicator - 1 tuber/nest and less. Only single hybrids were characterized by a low value of the coefficient of variation of the manifestation of the sign (3-9%). The nursing forms reacted differently to the external conditions by the ability to tie tubers. Key words: tubers, interspecific hybrids, their backcrosses, number of tubers in the nest, meteorological conditions, places of the experiments, coefficient of the variation.

Interdisciplinary approach to teaching physics to students majoring in agrarian engineering and agronomy

This study reports on interdisciplinary approach to teaching physics to the students majoring in agrarian and mechanical engineering. Physics plays a crucial role in mastering major disciplines by students of technical universities. Ukrainian higher education provides primarily mono-disciplinary approach to teaching academic and major subjects, so called teaching isolated disciplines. The interdisciplinary aspect of teaching physics corresponds to the requirements of modern society to educate competitive and qualified specialists in agricultural and engineering spheres. The study aims at defining the essence of interdisciplinary links between physics and major subjects, at specifying the peculiarities of job-focused physics tasks, at demonstrating the necessity of implementation the professional context in the process of studying. The paper investigates this issue by analyzing, comparing, systematizing the pedagogical literature.The study is based on monitoring and pedagogical experiment that proved the insufficient level of professional orientation of physics course. Pedagogical experiment was carried (out from 2014 to 2017). The students of State Agrarian and Engineering University in Podilya, Tavriyskiy State Agrarian University majoring in Agrarian Engineering and Agronomy and the students of Sumy National Agrarian University majoring in Agricultural mechanization took part in the survey. The sample involved 150 students. The study demonstrated such advantages of interdisciplinary approach to teaching physics through occupation-based task scenarios as student engagement and highly qualified specialists, subject interaction. The results of the experiment showed the insufficient level of professional competence of physics course. The material in this article, he patterns of physics course tasks accompanied by illustrations for students of mechanical engineering and agrarian departments, in particular, can be used in educational process.

Продуктивність міжвидових гібридів картоплі, їх беккросів залежно від умов випробування

The article presents data of evaluation of 33 complex interspecies hybrids of potatoes, their backcrosses by productivity as a result of testing of material in two places: Sumy National Agrarian University and Ustyimovskaya Research Station of the Institute of Plant Cultivation named V.Ya. Yuriev and for three years (2015-2017). The high potential of the material under study for productivity, with the maximum manifestation of the trait in the three-time backcrosess of the five-species hybrid 08.197/105-1643 g/bush, is 2.6 times higher than the best of the standards of the Javir variety. However, only in 2017, for tests in the SNA, the proportion of hybrids with very high productivity – more than 700 g /bush was 54.4%. It was found that the worst conditions for the realization of the sign were found in 2016 for assessments at the Ustyimovskaya Research Station. Expression of the indicator less than 200 g/nest had 60.6% hybrids. Significant influence of external conditions on the productivity of the investigated material is revealed. The largest share of hybrids with maximum productivity was detected in 2017 in the SNAU and in 2015 at the Ustyimovskaya Research Research Station. In both places, the test was very unfavorable for the manifestation of the sign was 2016, although individual hybrids in these conditions were characterized by the maximum expression of the indicator. Different reaction of standard varieties and test material on growing conditions is proved. The same share of hybrids with a higher manifestation of the trait than the best standard-grade was found in the SNAU (27.3%) and the Ustyimovskaya Research Research Station (9.1%) in 2015 and 2017. For both test sites, it was the same in 2016 (3.0%). By the proportion of hybrids with a large difference in the manifestation of productivity, depending on the places and years of research, the magnitude of the variation coefficient has been shown to have a significant effect of the external condition on the manifestation of the sign. The maximum value of the latter reached 88%, although in some hybrids it was within 5-10%. A different reaction was found to the outer complex of sister forms, which depended on the origin of the material