[1] We present vertically resolved thermospheric temperatures and NO abundances in terms of volume mixing ratio retrieved simultaneously from spectrally resolved 5.3 μm emissions recorded by the Michelson Interferometer for Passive Atmospheric Spectroscopy (MIPAS) in its upper atmospheric observation mode during 2005–2009. These measurements are unique since they represent the first global observations of temperature and NO for both day and night conditions taken from space. A retrieval scheme has been developed which accounts for vibrational, rotational and spin-orbit non-LTE distributions of NO. Retrieved polar temperature and NO profiles have a vertical resolution of 5–10 km for high Ap values, and degrade to 10–20 km for low Ap conditions. Though retrieved NO abundances depend strongly on the atomic oxygen profile used in the non-LTE modeling, observations can be compared to model results in a consistent manner by applying a simple correction. Apart from this, total retrieval errors are dominated by instrumental noise. The typical single measurement precision of temperature and NO abundances are 5–40 K and 10–30%, respectively, for high Ap values, increasing to 30–70 K for Tk and 20–50% for NO VMR for low Ap conditions. Temperature and NO profiles observed under auroral conditions are rather insensitive to smoothing errors related to the mapping of a priori profile shapes. However, for extra-polar and low Ap conditions, a potential systematic bias in the retrieved nighttime temperature and NO profiles related to smoothing errors has been identified from a comparison to Thermosphere Ionosphere Mesosphere Electrodynamics General Circulation Model (TIME-GCM) simulations. We have constructed a solar minimum monthly climatology of thermospheric temperature and NO from MIPAS observations taken during 2008–2009. MIPAS temperature distributions agree well, on average, with the Mass Spectrometer and Incoherent Scatter radar model (NRLMSISE-00), but some systematic differences exist. MIPAS temperatures are generally colder than NRLMSISE-00 in the polar middle thermosphere (mainly in the summer polar region) by up to 40 K; and are warmer than NRLMSISE-00 in the lower thermosphere around 120–125 km by 10–40 K. Thermospheric NO daytime distributions agree well with the Nitric Oxide Empirical Model (NOEM), based on Student Nitric Oxide Explorer (SNOE) observations. A comparison of MIPAS NO number density with the previous climatology for the declining phases of the solar cycle based on HALOE and SME data shows that MIPAS is generally larger with values ranging from 10 to 40%, except in the auroral region and at the equatorial latitudes above 130 km where the MIPAS/HALOE+SME ratio varies from 1.6 to 2. Day-night differences in MIPAS NO show daytime enhancements of up to 140% in the tropical and midlatitudes middle thermosphere. In the lower thermosphere, the diurnal amplitude is smaller and NO concentrations are generally higher during night by about 10–30%, particularly in the auroral regions.