Twenty-four multiparous Holstein cows were divided into six blocks of 4 cows based on their previous 305-d mature equivalent milk yield and were used in a 2 × 3 factorial experiment for the first 84 DIM. Objectives were to determine the effects of forage and RUP supplementation on DM and nutrient intakes and digestibilities, milk yield and composition, BW change, and plasma concentrations of insulin, triiodothyronine, thyroxine, cortisol, NEFA, urea N, and protein. One cow from each block was assigned to wheat or sorghum silage plus one of three concentrates. Protein treatments were 1) all supplemental CP from soybean meal; 2) 33.3% of supplemental CP from heated soybean meal, fish meal, and corn gluten meal; and 3) protein treatment 2 plus an additional 4.6% CP from soybean meal. Intakes of DM, CP, NDF, ADF, NEL, and RUP were higher in cows fed sorghum silage; RUP tended to increase DMI, Milk and 3.5% FCM yields were higher for cows fed sorghum silage than for those fed wheat silage (42.3 vs. 40.0 kg/d and 41.2 vs. 38.7 kg/d, respectively). Milk protein, lactose, and SNF were increased by RUP. Insulin was increased, cortisol was decreased, and thyroid hormones were unaffected by RUP. Concentrations of NEFA were highest at wk 4. For early lactation cows fed diets containing sorghum or wheat silage, RUP is a necessity.
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