Aiming at the problems existing in existing steganalysis algorithms, this article proposes Motion Vector Coding Cost Change video steganalysis features based on Improved Motion Vector Reversion-Based features and Subtractive Probability of Coding Cost Optimal Matching features based on Subtractive Probability of Optimal Matching features from the perspective of the change of coding cost. Motion Vector Coding Cost Change features can be well consistent with the coding cost before recoding by analyzing the sub-pixel coding cost of recoding. By counting the sub-pixel coding costs of motion vectors before and after video recoding, the Sum of Absolute Difference values of motion vectors instead of predicted residuals are applied to steganalysis and detection, and the steganographic algorithm based on motion vectors is effectively detected. Experiments show that Motion Vector Coding Cost Change features have higher detection accuracy than Add-or-Subtract-One, Improved Motion Vector Reversion-Based, and other typical features in various steganography methods, and Subtractive Probability of Coding Cost Optimal Matching features have higher detection effect and better robustness than Subtractive Probability of Optimal Matching features.