Sulfate Heptahydrate Research Articles

Bio-inspired synthesis of ZnO nanoparticles using Taraxacum officinale for antibacterial, antifungal, and anticancer applications

A green synthesis method was developed to fabricate ZnO nanomaterials using Taraxacum officinale (Dandelion) leaf extract as a reducing agent and zinc sulfate heptahydrate as a precursor. Characterization by XRD, EDAX, FTIR, FESEM, and UV–vis spectroscopy confirmed the successful formation of ZnO nanocrystals with enhanced crystallinity following annealing. The ZnO nanomaterials exhibited significant bioactivity, demonstrating 85 % antibacterial efficiency against Escherichia coli and Staphylococcus aureus, along with 70 % antifungal activity. Furthermore, the nanomaterials showed promising anticancer effects, achieving a 60 % reduction in human cancer cell viability. This eco-friendly approach minimizes the use of toxic chemicals typically involved in nanoparticle synthesis, providing a sustainable alternative for producing functional oxide nanoparticles. The use of plant-based reducing agents not only makes the process safer and more biocompatible but also enhances the potential for biomedical applications. ZnO nanomaterials produced through this method offer great promise for antimicrobial therapies and cancer treatment. This efficient, green synthesis method can be further scaled for various applications, highlighting its relevance in fields such as nanomedicine, where bioactivity and eco-friendly synthesis are essential.

Optimisation, Synthesis, and Characterisation of ZnO Nanoparticles Using Leonotis ocymifolia (L. ocymifolia) Leaf Extracts for Antibacterial and Photodegradation Applications.

This work presents a green synthesis route, which utilises extracts from an indigenous plant in South Africa, eastern and southern Africa that is understudied and underutilised, for preparing zinc oxide nanoparticles (ZnO NPs). This study involved optimisation of the green synthesis method using Leonotis ocymifolia (L.O.) extracts and performing comparative studies on the effects of using different zinc (Zn) salt precursors; zinc sulphate heptahydrate (Z001) and zinc acetate dihydrate (Z002) to synthesise the ZnO NPs. The comparative studies also compared the L.O-mediated ZnO NPs and chemical-mediated ZnO NPs (Z003). The as-prepared ZnO NPs were tested for their effectiveness in the photodegradation of methylene blue (MB) dye. Furthermore, antibacterial studies were conducted using the agar well diffusion method on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria. The structural, morphological, and optical characteristics of the synthesised ZnO NPs were analysed using XRD, FTIR, SEM, EDS, DRS, and BET techniques. The XRD results indicated that the L.O-mediated ZnO NPs had smaller crystallite sizes (18.24-19.32 nm) than their chemically synthesised counterparts (21.50 nm). FTIR confirmed the presence of biomolecules on the surface of the L.O-mediated NPs, and DRS analysis revealed bandgap energies between 3.07 and 3.18 eV. The EDS results confirmed the chemical composition of the synthesised ZnO NPs, which were made up of Zn and O atoms. Photocatalytic studies demonstrated that the L.O-mediated ZnO NPs (Z001) exhibited a superior degradation efficiency of the MB dye (89.81%) compared to chemically synthesised ZnO NPs (56.13%) under ultraviolet (UV) light for 240 min. Antibacterial tests showed that L.O-mediated ZnO NPs were more effective against S. aureus than E. coli. The enhanced photocatalytic and antibacterial properties of L.O-mediated ZnO NPs highlight their potential for environmental remediation and antimicrobial applications, thus supporting sustainable development goals.

Optimization of Fermentation and Biocontrol Efficacy of Bacillus atrophaeus XHG-1-3m2

Biological control plays an increasingly important role in various aspects of modern agriculture and forestry. Identifying biocontrol strains with commercial potential for effective disease management is currently a focal point in biological control research. In this study, Bacillus atrophaeus XHG-1-3m2, a strain with significant biocontrol potential against Wilsonomyces carpophilus causing shot hole disease in wild apricots, was developed. The study determined the antibacterial activity of the fermentation broth, the optimal fermentation medium composition and conditions, and explored its effectiveness in controlling Wilsonomyces carpophilus. The optimal fermentation medium for strain XHG-1-3m2 comprises 12.5 g/L yeast extract, 12.5 g/L soy peptone, 10.0 g/L sodium chloride, 1 g/L ammonium chloride, 1 g/L potassium dihydrogen phosphate, 1 g/L disodium hydrogen phosphate, and 0.5 g/L magnesium sulfate heptahydrate. With an initial pH of 7.0, a liquid volume of 40%, an inoculum volume of 3%, and shaking incubation at 28 °C for 24 h, the viable cell count reached 14 × 109 CFU/mL. In vitro and in vivo tests on leaves revealed that the fermentation broth and the biocontrol biofertilizer derived from this strain inhibited the leaf lesions caused by Wilsonomyces carpophilus on wild apricots, achieving inhibition rates of 94.62% and 82.46%, respectively.

Eco-Friendly Dyeing Processes of Nylon 6.6 Woven Fabrics with Used Coffee Grounds (UCG)

The increasing demand for sustainable practices in the textile industry has led to the exploration of natural dyes and eco-friendly dyeing processes. This study focuses on the potential of used coffee grounds (UCG) as an eco-friendly natural dye for Nylon 6.6 woven fabrics. Five dyeing processes were evaluated, varying in the use of mordants and acids, to assess their impact on the color saturation, colorfastness to laundering, and crocking resistance of Nylon 6.6. fabric. The processes included a control with no mordant or acid and others that incorporated tannic acid, acetic acid, and ferrous sulfate heptahydrate. The results demonstrated that process 4, which combined tannic acid pre-mordanting with acetic acid in the dye bath, provided the best balance between color saturation and colorfastness. Process 2, utilizing only tannic acid, offered some durability in laundering and crocking tests. Process 5, being the least eco-friendly process, demonstrated high color saturation, but it performed poorly in colorfastness to crocking, which means that it released the UCG-based dye after rubbing the dyed Nylon 6.6. fabric. The findings confirm that UCG can be an effective and sustainable natural dye for Nylon 6.6, with pre-mordanting and acid treatment significantly enhancing dye uptake and retention. However, further research is needed to optimize color intensity and expand the application of UCG in textile dyeing.

Biosynthesis of Iron Oxide Nanoparticles by Marine Streptomyces sp. SMGL39 with Antibiofilm Activity: In Vitro and In Silico Study

One of the major global health threats in the present era is antibiotic resistance. Biosynthesized iron oxide nanoparticles (FeNPs) can combat microbial infections and can be synthesized without harmful chemicals. In the present investigation, 16S rRNA gene sequencing was used to discover Streptomyces sp. SMGL39, an actinomycete isolate utilized to reduce ferrous sulfate heptahydrate (FeSO4.7H2O) to biosynthesize FeNPs, which were then characterized using UV–Vis, XRD, FTIR, and TEM analyses. Furthermore, in our current study, the biosynthesized FeNPs were tested for antimicrobial and antibiofilm characteristics against different Gram-negative, Gram-positive, and fungal strains. Additionally, our work examines the biosynthesized FeNPs’ molecular docking and binding affinity to key enzymes, which contributed to bacterial infection cooperation via quorum sensing (QS) processes. A bright yellow to dark brown color shift indicated the production of FeNPs, which have polydispersed forms with particle sizes ranging from 80 to 180 nm and UV absorbance ranging from 220 to 280 nm. Biosynthesized FeNPs from actinobacteria significantly reduced the microbial growth of Fusarium oxysporum and L. monocytogenes, while they showed weak antimicrobial activity against P. aeruginosa and no activity against E. coli, MRSA, or Aspergillus niger. On the other hand, biosynthesized FeNPs showed strong antibiofilm activity against P. aeruginosa while showing mild and weak activity against B. subtilis and E. coli, respectively. The collaboration of biosynthesized FeNPs and key enzymes for bacterial infection exhibits hydrophobic and/or hydrogen bonding, according to this research. These results show that actinobacteria-biosynthesized FeNPs prevent biofilm development in bacteria.

Thermal and exergy analysis of solar air heater enhanced with sodium carbonate decahydrate and Magnesium sulfate heptahydrate PCM: Performance evaluation

The advancement of renewable solar energy is prospective for energy, heat exchanger, and air heater applications. The main objective of the present research is enhancing the solar air heater thermal performance by the modification of a collector absorber-like plain absorber featuring rectangular fine (SAH 2) and sodium carbonate decahydrate (Na2CO3·10H2O) and Magnesium sulfate heptahydrate (MgSO4·7H2O) PCM with rectangular fin (SAH 3). During the experimental evaluation, the airflow varied by 0.042 and 0.059 kg/s. Influences of collector modification on the functional behaviour of solar air heaters (SAH) are evaluated. Its results are compared to the system of conventional setup without modification of the solar collector with a plain absorber (SAH-1). With the excellence of Na2CO3·10H2O/MgSO4·7H2O with fin setup performed with 0.059 kg/s found better heat transfer performance including heat gain, thermal & exergy efficiency, heat loss coefficient, and PCM temperatures like 927.3 W, 78.6 %, 33.6 %, 16.9 W/m2K, and 68.3 ⁰C respectively. Moreover, Increasing the airflow rate leads to improved heat transfer, and the SAH proves to be effective at higher flow rates when equipped with fins and PCM.

Effect of zinc management on yield, nutrient content, and acquisition of zero-tilled wheat (Triticum aestivum)

A field experiment was conducted during the winter (rabi) season 2018–19 and 2019–20 at Khalsa College, Amritsar, Punjab to find out the effect of soil and foliar application of ZnSO4 .7H2 O on zero till wheat. The experi- ment was conducted in a randomized complete block design with three replications. Treatments were control (noZn), soil application of 12.5, 25, 37.5, 50 kg/ha zinc sulfate heptahydrate (ZnSO4 .7H2 O), and foliar application of 0.5% Zn as one spray at heading initiation (5% ear emergence), one spray at 100% heading (complete ear emergence) and two sprays at heading initiation and 100% heading with a recommended dose of fertilizer. Results showed that the soil application of 50 kg Zn/ha with two foliar sprays of 0.5% zinc sulfate heptahydrate at heading initiation and 100% heading produced the highest yield attributes and grain yield of wheat. Application of 50 kg Zn/ ha with two foliar sprays of 0.5 % Zn at heading initiation and 100% heading registered ~25% higher grain yield over control. Grain Zn content (41.77ppm) percent increase was 33.9 with 50 kg Zn/ha with two foliar sprays of 0.5 % zinc at heading initiation and 100% heading over control (31.20 ppm).Likewise, application of 50 kg Zn/ha with two foliar sprays of 0.5 % Zn at heading initiation and 100% heading recorded the highest net returns among the Zn treatments. Overall, soil application of 50 kg Zn/ha with two foliar sprays of 0.5 % zinc at heading initiation and 100% heading improved the yield, uptake, and profitability of zero-tilled wheat in zinc-deficient soils.

Di-μ-adipato-κ4 O 1,O 1':O 6,O 6'-bis-[(2,2'-di-pyridyl-amine-κ2 N,N')zinc(II)] trihydrate.

The title compound, [Zn2(C6H8O4)2(C10H9N3)2]·3H2O or {Zn2[(C5H4N)2NH]2[μ-(CH2)4(COO)2]2}·3H2O, was separ-ated from the solvothermal reaction of zinc(II) sulfate hepta-hydrate, 2,2'-di-pyridyl-amine and sodium adipate. The dinuclear metal complex has a centrosymmetric structure, with the ZnII atom adopting a highly distorted octa-hedral coordination sphere composed of four oxygen atoms from bridging adipato ligands and two pyridine nitro-gen atoms. In the crystal, the title compound aggregates into a tri-periodic supra-molecular structure through inter-molecular hydrogen-bonding networks of the form O-H⋯O and N-H⋯O.

Ecofriendly approaches to efficiently enhance catalase performance

The present work reports on two approaches to enhance catalase (CAT) activity and its stability by using two simple, green processes. In the first procedure, CAT was transiently exposed to an ionic liquid (IL) in the presence of redox molecules related to CAT structure which resulted in partial denaturation. The other method, which uses high hydraulic pressure (HHP) to partially denature CAT (in the presence of redox molecules), has the advantage of being completely reagentless. In both cases, partial denaturation was followed by dialysis, hence refolding and entrapment of redox molecules within the modified 3-D CAT structure (affording a “wired” enzyme). The two approaches to enzyme “wiring” are discussed comparatively from the point of view of the parameters used during the procedure, residual enzyme activity, nature of the modifier, interaction between CAT and the redox molecules, antioxidant activity, and stability over time of the modified protein. Samples of CAT modified in the presence of iron sulfate heptahydrate from each series, respectively, were used to make enzyme electrodes which were tested as amperometric biosensors for hydrogen peroxide detection. Both showed catalytic effect and linear behavior and have potential for applications in the food industry, pharmaceuticals and the textile industry.

Nitrogen Requirement and Nitrogen Form Preference by Radermachera hainanensis and R. sinica Plug Seedlings

Abstract Radermachera hainanensis Merr. and R. sinica Hemsl. are popular foliage plants raised from seeds. Nitrogen fertilizer can significantly influence medium pH and EC, plug seedling growth, and photosynthesis. Plug seedlings of both species, each with one pair of leaves, were subjected weekly to nutrient solutions with nitrogen (N) concentrations ranging from 0 to 32 mM. Nitrogen-deficient plants exhibited the lowest leaf area, SPAD-502 value, and shoot dry weight but the highest root-to-shoot ratio. Leaf area, SPAD-502 value, and root and shoot dry weights increased with increasing N concentration up to 12-16 mM, and gradually decreased thereafter. Well-grown plants with 12-16 mM N exhibited peak maximal fluorescence (Fm), photochemical quenching (qP) and the lowest minimal fluorescence (Fo) and non-photochemical quenching (qN). Seedlings received nutrient solutions with varying proportions of NH4-N: NO3-N (0:100, 25:75, 50:50, 75:25, and 100:0, in percentages) at 16 mM N. Both species exhibited reduced leaf area, SPAD-502 value, and plant dry weights when supplied with only NO3-N or NH4-N. Maximum growth was achieved at a 50% NH4-N: 50% NO3-N ratio. Species used in this study: Golden jasmine tree, Radermachera hainanensis Merr.; China Doll, Radermachera sinica Hemsl. Chemicals used in this study: ammonium nitrate; sodium dihydrogen phosphate; potassium sulphate; potassium nitrate; magnesium sulfate heptahydrate; calcium sulfate dihydrate; calcium nitrate tetrahydrate; ammonium sulfate; boric acid anhydrous; potassium chloride; ferrous sulfate heptahydrate; disodium ethylenediaminetetraacetic acid dihydrate; copper sulfate pentahydrate; zinc sulfate heptahydrate; molybdic acid; manganese sulfate monohydrate; nickel sulfate hexahydrate; sodium hydroxide.

Synthesis, evaluation, and biocompatibility study of magnetite nano particles in normal cells and cancer cells for health care application

Magnetic nanoparticles have been synthesized in a very simple and economical way by the co-precipitation method, where the effect of molar concentrations of ferric chloride anhydrous (FeCl3) and iron (II) sulphate heptahydrate (FeSO4.7 H20) on magnetite synthesis has been investigated. Also, a detailed study was conducted to study the effect of magnetite and hematite on both normal and cancerous cell lines. After this, the magnetic nanoparticles obtained were analyzed by x-ray Diffraction (XRD), scanning electron microscope (SEM) - energy dispersive x-ray (EDX), Fourier transform infrared spectroscopy (FTIR), zeta potential, vibrating sample magnetometer (VSM), atomic force microscopy (AFM), MTT test, and cell apoptotic assay. The XRD peaks for magnetite were easily discernible in the final formulation. SEM images showed round particles in nano ranges, and FTIR peaks showed the presence of magnetite. Zeta potential showed surface charges. VSM showed the magnetic property of magnetite, and AFM confirmed SEM images. It can be concluded that magnetic nanoparticles were synthesized by the co-precipitation method using an optimized molar concentration of reagents. Also, the necessity of coating uncoated magnetic nanoparticles can be seen from the MTT assay. Cell apoptotic assays have shown that synthesized magnetite nanoparticles have shown potential apoptotic activity on cancer cell lines.

Kinetic study on the thermal decomposition of iron (II) sulfate using a global optimization approach

The thermal decomposition of sulfates is a major intermediate reaction in oxidizing roasting processes associated with metal sulfides. The optimization of iron (II) sulfate could contribute to lowering processes’ temperatures in complex systems. The present work presents the experimental and modeling results of the thermal decomposition of iron (II) sulfate heptahydrate. The investigation was first based on thermodynamic simulations followed by non-isothermal thermogravimetric analysis. The modeling technique consisted of differential equations formulated to describe each decomposition step individually, with the kinetic parameters estimated using particle swarm optimization (PSO). Using this modeling methodology over a graphical method is advantageous as it can simulate reactions that may occur simultaneously. The modeling succeeded in dehydration and desulfation steps, with an overall R2 value of 0.99. The activation energies and apparent reaction order associated with the dehydration steps were 53.3kJ.mol−1/n=1.65, 88.8kJ.mol−1/n=1.12, and 124kJ.mol−1/n=2.0 for FeSO4.7H2O, FeSO4.4H2O, and FeSO4.H2O, respectively. The desulfation steps were associated with higher activation energy values. In that case, 184kJ.mol−1 and 264kJ.mol−1 for FeSO4 and Fe2(SO4)3, respectively. Regarding the apparent reaction order, both desulfation steps showed to be first-order reactions.

Enhancement in Formation of Insoluble Arsenic Phases without Re-Leaching by Particle Size of Excavated Sediment with Iron Sulfate Heptahydrate

ABSTRACT Massive quantities of naturally arsenic-containing sediments are excavated for construction. Chemical immobilization is applied to such excavated materials for reuse. Appropriate physicochemical properties should be understood to enhance the formation of the insoluble arsenic phases without re-leaching. The up-flow column retention test flowing 10 mg L−1 of arsenic solution was conducted to understand how the retention of arsenic and the phases of arsenic immobilized by iron sulfate heptahydrate depend on the particle size of the excavated sediment. The amount of arsenic retained on the immobilization material (particle size of 0.5 mm) during the retention test was lower for the larger particle size of the excavated sediment. However, arsenic was more stably retained on the immobilization material for the large particle sizes (1.0–2.0 and 2.0–5.0 mm) and the amount of arsenic re-leached was 12% of that in the small one (<0.5 mm). The percentages of arsenic in fraction 3 of sequential extraction increased from 45% to 68% with increasing the particle size. These results indicate that the excavated sediment with a larger particle size than that of immobilization material retains less arsenic on the immobilization material, but the arsenic phases immobilized are more stable, resulting in less arsenic re-leaching. This study suggests that the use of excavated sediment with a particle size larger than that of the immobilization material is desirable for the application of immobilization technique without re-leaching.

Cellulose Nanofiber-Based Nanocomposite Films with Efficient Electromagnetic Interference Shielding and Fire-Resistant Performance.

Cellulose nanofiber (CNF) has been widely used as a flexible and lightweight polymer matrix for electromagnetic shielding and thermally conductive composite films because of its excellent mechanical strength, environmental performance, and low cost. However, the lack of flame retardancy seriously hinders its further application. Herein, renewable and biomass-sourced l-arginine (AR) was used to surface-modify ammonium polyphosphate (APP) and an environmentally friendly biobased flame retardant was synthesized by the coordination of zinc sulfate heptahydrate (ZnSO4·7H2O), which was named AAZ. AAZ was deposited on the surface of CNF by electrostatic adsorption and Zn2+ complexation. The biobased compatibilizer Triton X-100 was employed to assist the exfoliation of graphene nanoplatelets (GNPs) and their dispersion in the CNF matrix. Due to the formation of a dense lamellar layer resembling a shell structure, the CNF/GNPs composite films with a tensile strength of 52 MPa were obtained via vacuum-assisted filtration. Because the phosphorus-containing group produces a protective layer of PxOy compound and promotes the formation of a carbon layer by CNF and the combustion releases ammonia gas, the fire-resistant performance of the composite films was greatly improved. Compared with the pure CNF film, the composite film exhibits 33% reduction in PHRR value and 40% reduction in THR. In addition, the CNF/GNPs composite film with 20 wt % GNPs possessed high conductivity (2079.2 S/m) and electromagnetic interference (EMI) shielding effectiveness (37 dB). The ultrathin CNF/GNPs composite films have excellent potential for use as efficient flame retardant and EMI shielding materials.

Study on the Preparation and Compressive Strength of Boron Mud-Based Basic Magnesium Sulfate Cement.

The direct discharge of boron mud poses significant environmental hazards to soil and groundwater. Despite extensive research efforts, the reprocessing of boron mud has not yielded significant advancements. Recently, the development of magnesium cement has spurred interest in the reutilization of boron mud. However, the direct treatment of boron mud remains challenging, necessitating pre-treatment in most studies to achieve substantial results. Consequently, research on the direct incorporation of untreated boron mud is scarce. This study explores the feasibility of using uncalcined boron mud as a base material in basic magnesium sulfate cement (BMSC), composed of lightly calcined magnesia and magnesium sulfate heptahydrate. The effects of varying boron mud content on the compressive strength of the BMSC system were investigated. The results indicate that the 5·1·7 phase is the primary strength phase of BMSC. When the boron mud content is 30%, the uncalcined boron mud has a minimal impact on the formation of the 5·1·7 phase. Additionally, the 28 days compressive strength of BMSC-B30 showed a slight difference compared to the control group BMSC-C, registering at 66.7 MPa. TG-DSC analysis revealed that the presence of a small amount of boron mud inhibits the micro-expansion trend of the BMSC structure. Furthermore, XRD and SEM analyses confirmed that the addition of uncalcined boron mud does not significantly alter the phase structure of the 5·1·7 phase in BMSC. This study provides a foundational basis for the long-term development of direct boron mud treatment.

The Application of Tannic Acid-Coated Magnetite Nanoparticles for Recovery of Microplastics from the Water System

The presence of microplastics (MPs) is rapidly increasing in the environment, posing a significant threat to public health and the ecosystem. To monitor the impact of MPs, it is crucial to have standardized detection methods and MPs remediation techniques. Therefore, this study aimed to use tannic acid-coated magnetite nanoparticles to recover MPs, specifically polystyrene (PS) and polyethylene terephthalate (PET), from water bodies. A facile method for MPs recovery was established using the synthesized tannic acid-coated magnetite (TA-Fe3O4) as an adsorbent. TA-Fe3O4 was synthesized using tannic acid, iron (II) sulfate heptahydrate, and iron (III) chloride hexahydrate through a one-pot co-precipitation method. The adsorbent material was then characterized using a scanning electron microscope coupled with an energy dispersive x-ray spectroscopy (SEM–EDS), x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, zeta potential, and N2 adsorption–desorption (BET) techniques, confirming its successful synthesis. The maximum adsorption efficiencies for PS and PET were 83% and 98%, respectively, under the experimental conditions of pH 6–7, a contact time of 300 min, an adsorbent dose of 2.5 g/L, and an initial MPs concentration of 1.25 g/L. The study also found that the presence of ions in wastewater had a significant effect on MP recovery. However, when it came to recovering MPs from the spent adsorbent, the maximum values were observed in an acidic medium at pH 3–4 under sonification conditions. In conclusion, TA-Fe3O4 showed potential and efficiency in the recovery of MPs in aqueous media. However, further investigation is needed to improve the method and explore its application for multi-MPs recovery performance.

Effect of pH on Toxicity of Zinc Sulphate Hydrate Solutions Using the Spirotox Test

Introduction: Spirostomum ambiguum is commonly used as a biological indicator due to its high sensitivity to heavy metals. It functions normally across a wide pH range of 4.5 to 8.0. Testing on protozoan organisms provides a general understanding of the impact of pH on the toxicity of zinc in pharmaceuticals. By investigating the toxicity of zinc in environment with varying pH levels, the optimal environment for zinc absorption can be determined. Objective: The objective of this study is to assess the toxicity of zinc sulphate solutions using different forms of hydrating and at various pH levels, applying the Spirotox method. Methods: An experiment was conducted using a solution of pharmaceutical substances, specifically zinc sulphate heptahydrate and zinc sulphate monohydrate, with a concentration of 0.01 M. The study aimed to investigate the effect of the solution on the lifespan of S.ambiguum within the pH range of 4.8-6.5. Results: Arrhenius activation energy (Ea linear correlates with LD50 oral toxicity for rats) decreases as pH increases from 4.8 to 6.5 for ⋅7 and ⋅ solutions. The values decrease from 140±10 kJ/mol to 55±10 kJ/mol and from 110±2 kJ/mol to 60±10 kJ/mol, respectively. Conclusion: It was found that the toxicity of zinc sulphate hydrate solution towards S. ambiguum increased as the pH value increased. Hydrating of the active pharmaceutical substance was a crucial factor, it is critically important for preparation of solutions of an active pharmaceutical substance. Keywords: zinc sulphate, Spirotox test, pH, activation energy, toxicity.

Colchicine-capped ZnO nanoparticles: Elucidation of their cytotoxicity potential against MDA-MB 231 cancer cells

This research descibes a pioneering approach aimed at preparing zinc oxide nanoparticles (ZnO-NPs) with colchicine as the reducing and capping agent. Colchicine-loaded ZnO-NPs (CHZnO-NPs) were prepared by adding colchicine to the zinc sulfate heptahydrate solution. The CHZnO-NPs formulation was then characterized to determine the morphology, size, crystallinity, elemental composition and vibrational properties. Upon characterization, CHZnO-NPs were studied for their cytotoxic effect against the breast cancer cell line (MDA-MB-231). The successful biosynthesis of CHZnO-NPs was initially confirmed visually by the changes in the mixture color, from light-yellow to white cloudy. The best CHZnO-NPs formulation selected was F3, which possessed 10 mg/mL of colchicine. Formulation (F3) had the smallest mean particle diameter of 43.77 nm and the lowest zeta potential of −19.60 mV. It also had 92.21 ± 0.012 % encapsulation efficiency and 20.86 ± 0.005 % drug loading. Formulation (F3) displayed a hexagonal wurtzite structure with irregular morphology. The observation of colchicine peaks on the FTIR spectra of F3 proved the role of colchicine as a reducing and capping agent during the synthesis of ZnO-NPs. Besides, the in-vitro cell cytotoxicity study on the MDA-MB-231 cell line revealed a significant reduction in cell proliferation at the concentration of 25 μg of colchicine and F3. Further, studies on the cellular migration potential also demonstrated concentration-dependent activity. Overall, CHZnO-NPs were shown to be successfully synthesized via an environmental-friendly procedure and colchicine acted as a capping agent to regulate the particle size, and aggregation, in addition to its anticancer properties.

Contribution of Plant Transfer Printing to Sustainable Fashion

Nowadays, there is a growing awareness of environmental protection, new findings in the field of sustainable chemistry, the use of biodegradable materials, and the increased use of eco-friendly textile products. For this reason, natural dyes are being used more and more frequently, giving rise to a new way of decorating textiles, namely, plant transfer printing, popularly known as “eco-printing”, in which the shape and/or pigment of a plant is transferred to the textile. In addition, the great interest of the young generation in the application and research into the use of natural dyes can create incentives for cultural and social sustainability through the preservation of national heritage. Plant transfer printing is a method that combines scientific technology and artistic design with corresponding benefits for the eco system. The very fact that the patterns are unique and unpredictable brings out the notion of artistic freedom. In the work, plant transfer printing was carried out on undyed cotton material and on material dyed with pomegranate peels, walnut leaves, coffee, and aleppo pine bark. The influence of the pH value and the capillarity of the fabric, as well as the treatment of the leaves with iron(II) sulphate heptahydrate solution, on the aesthetics of the print and the colour fastness during washing was investigated. Based on the optimised parameters and a sustainable fabric design, the clothing collection “Hamadryad”, inspired by Greek mythology, was realised.

Optimization of industrial Fenton process of phenolic effluent using artificial neural network and design of experiments

This work aimed to optimize the homogeneous Fenton process used to remove phenol in a chemical industry located in Mauá (São Paulo, Brazil). Phenolic effluent exhibits significant variability in its chemical composition, which poses a great challenge in finding the optimal reagents ratio for conducting Fenton treatment. Often, there is a need for repetitions to treat the same batch, leading to increased reagent consumption and treatment time. Thus, to optimize the industrial process, the present work applied modern optimization techniques on real data, such as design of experiments (DoE) and artificial neural network (ANN). ANN was used to perform simulations in the company’s process, thereby predicting the quantities of ferrous sulfate heptahydrate and hydrogen peroxide. The following optimal conditions have been determined by DoE (phenol concentration ≤ 4 ppm): pH from 2.7 to 4 and temperature from 28 to 40 °C. Before the implementation of the optimized parameters, the efficiency for a single treatment was 45%. After, the efficiency was 82% and the company saved, on average, 51.6% of reagent costs. The main contribution of the present work is the development and industrial testing of data science methods that aims to optimize the industrial Fenton process.