The results of extraction of chondroitin sulfuric acid in a water solution by various amines in butanol and the effects of certain conditions on the extraction are briefly presented. A method of column chromatography was developed for fractionating hyaluronic acid and chondroitin sulfuric acid and for separating mixtures of these substances. The materials isolated from the columns showed no differences in composition from purified preparations, but showed differences in reduced viscosity.