Sulfur Deficiency Research Articles

Generation and characterization of single and multigene Arabidopsis thaliana mutants in LSU1-4 (RESPONSE TO LOW SULFUR) genes

In Arabidopsis thaliana, there are four members of the LSU (RESPONSE TO LOW SULFUR) gene family which are tandemly located on chromosomes 3 (LSU1 and LSU3) and 5 (LSU2 and LSU4). The LSU proteins are small, with coiled-coil structures, and they are able to form homo- and heterodimers. LSUs are involved in plant responses to environmental challenges, such as sulfur deficiency, and plant immune responses. Assessment of the role and function of these proteins was challenging due to the absence of deletion mutants. Our work fulfills this gap through the construction of a set of LSU deletion mutants (single, double, triple, and quadruple) by CRISPR/Cas9 technology. The genomic deletion regions in the obtained lines were mapped and the level of expression of each LSUs was assayed in each mutant. All lines were viable and capable of seed production. Their growth and development were compared at several different stages with the wild-type. No significant and consistent differences in seedlings’ growth and plant development were observed in the optimal conditions. In sulfur deficiency, the roots of 12-day-old wild-type seedlings exhibited increased length compared to optimal conditions; however, this difference in root length was not observed in the majority of lsu-KO mutants.

N2-Acetylornithine deacetylase functions as a Cys-Gly dipeptidase in the cytosolic glutathione degradation pathway in Arabidopsis thaliana.

Glutathione (GSH) is required for various physiological processes in plants, including redox regulation and detoxification of harmful compounds. GSH also functions as a repository for assimilated sulfur and is actively catabolized in plants. In Arabidopsis, GSH is mainly degraded initially by cytosolic enzymes, γ-glutamyl cyclotransferase, and γ-glutamyl peptidase, which release cysteinylglycine (Cys-Gly). However, the subsequent enzyme responsible for catabolizing this dipeptide has not been identified to date. In the present study, we identified At4g17830 as a Cys-Gly dipeptidase, namely cysteinylglycine peptidase 1 (CGP1). CGP1 complemented the phenotype of the yeast mutant that cannot degrade Cys-Gly. The Arabidopsis cgp1 mutant had lower Cys-Gly degradation activity than the wild type and showed perturbed concentrations of thiol compounds. Recombinant CGP1 showed reasonable Cys-Gly degradation activity in vitro. Metabolomic analysis revealed that cgp1 exhibited signs of severe sulfur deficiency, such as elevated accumulation of O-acetylserine (OAS) and the decrease in sulfur-containing metabolites. Morphological changes observed in cgp1, including longer primary roots of germinating seeds, were also likely associated with sulfur starvation. Notably, At4g17830 has previously been reported to encode an N2-acetylornithine deacetylase (NAOD) that functions in the ornithine biosynthesis. The cgp1 mutant did not show a decrease in ornithine content, whereas the analysis of CGP1 structure did not rule out the possibility that CGP1 has Cys-Gly dipeptidase and NAOD activities. Therefore, we propose that CGP1 is a Cys-Gly dipeptidase that functions in the cytosolic GSH degradation pathway and may play dual roles in GSH and ornithine metabolism.

Sulfonated nitrogen and added-sulfur sources influence productivity, quality, and nutrient acquisition of soybean-wheat cropping system

Soybean-wheat is the predominant cropping system covering >2.5 Mha area in India. The lower productivity of soybean-wheat cropping system (SWCS), remains a serious concern primarily due to inadequate nutrient management. Increasing sulfur (S) deficiency is widespread, especially under oilseed-based cropping system. Hence, to standardize the S requirement through customized fertilization, an experiment was conducted in completely randomized block design (RBD) comprised of 12 nutrient sources, replicated thrice. The study aims to evaluate the agronomic performance of sulfonated nitrogen (SN) in comparison to conventional S nutrient sources in SWCS. The maximum soybean productivity was recorded under NPK + S through 40-0-0-13 (SN1), although NPK + 50% S (15 kg/ha) as basal and 50% (15 kg/ha) as top dressing through SN2 10-0-0-75 produced maximum wheat grain yield. When compared with no nitrogen (control), the application of 30 kg S ha−1 to both crops increased the productivity of the soybean-wheat cropping system up to 39%. The maximum system (SWCS) productivity (8.45 tha-1) was obtained with the application of 50% S as basal and 50% as top dressing (SN2-based), remaining N through urea. The highest sustainable yield index of soybean (SYIS), i.e. 0.90 was under SN1+ remaining N through urea and likewise highest sustainable yield index of wheat (SYIW) was under S splitting. The application of SN also improved the nutrient acquisition and grain quality of soybean and wheat with a positive nutrient balance in the soil. The protein content and yield of soybean and wheat grains also improved. The higher gluten content in wheat grain was produced with 60 kg S ha−1 applied. The agronomic efficiency of N and S (AEN and AEs) were highest under SN1 and SN2, respectively (32.8 kg grain/kg N applied; 15 kg grain/kg S applied) in soybean, however in wheat, S splitting and urea application resulted in highest agronomic efficiency (AEN and AES) of N and S (17.1 kg grain/kg N applied; 22.3 kg grain/kg S applied respectively). Hence splitting of S doses of SN along with urea and recommend P, K was found efficient for the soybean-wheat cropping system.

Growth of freshwater cyanobacterium Aphanizomenon sp. ULC602 in different growing and nutrient conditions.

Aphanizomenon sp. ULC602, recently isolated in a Belgian lake, is a filamentous, nitrogen-fixing, freshwater cyanobacterium that is one of the primary producers of cyanotoxins following its bloom formation, causing water contamination. This study aims to evaluate the effects of growing conditions and essential nutrients on the growth of Aphanizomenon sp. ULC602 via its production of chlorophyll-a (Chlo-a). Our results indicated that this bacterium could grow well at temperatures ranging from 18 to 25°C with an optimal pH of 6.0-7.5 under continuous lighting. It grew slowly in the absence of a carbon source or at lower carbon concentrations. The addition of nitrogen from nitrate and urea led to a less than 50% reduction of Chlo-a content compared to the medium lacking nitrogen. The iron bioavailability significantly stimulated the Chlo-a production, but it was saturated by an iron concentration of 0.115 mM. Moreover, a decrease in Chlo-a biomass was observed under sulfur deficiency. The bacterium could not grow well in media containing various phosphorus sources. In conclusion, as the growth and consequent forming bloom of cyanobacteria can be stimulated or inhibited by environmental conditions and eutrophication, our investigation could contribute to further studies to control the blooming of the target bacterium in freshwater.

A novel sulfur supply strategy for maximizing lipid production in Tribonema minus (Xanthophyceae)

Tribonema minus, a promising filamentous oleaginous microalga, was cultured under different nutrient concentrations and different culture modes (fed-batch culture, two-step culture) to study the method of rapid regulation of its lipid metabolism. In contrast to many other oleaginous microalgae, T. minus did not show that nitrogen stress promoted lipid accumulation; however, sulfur deficiency promoted rapid lipid accumulation with a maximum lipid content of 54% of dry weight. Increasing the MgSO4 concentration significantly increased nitrogen uptake and biomass (10.09 g/L). Lipid productivity was significantly increased by the two-step culture using a medium with a high concentration of MgSO4 in the first step and a sulfur-free medium in the second step. In addition, it was found that the lipid content of T. minus was negatively correlated with the intracellular sulfur content when the intracellular sulfur content was below 0.6%. This study provides a new approach for industrial lipid production in T. minus.

The advantages of methanol-amine solvent mixtures in solution processing of Ge-Sb-S chalcogenide glass thin films

We report on the substantial influence of methanol presence on the dissolution mechanism of Ge20Sb5S75 bulk glass with subsequent superior properties of thin films deposited from such solutions. Raman spectroscopy confirmed significant differences in structural features present in glass solutions prepared from pure amines (n-propylamine and n-butylamine) and their mixtures with methanol. The experiments with dissolved elemental sulfur and Ge25S75 glass analog proved that both antimony and methanol presence induce further splitting of the Ge4S104− cluster structure, which fundamentally affects the properties of deposited thin films. Significant structural and compositional differences were found not only in solutions and as-prepared samples, but also after thin films’ thermal treatment (hard baking up to 210 °C). The as-prepared thin films deposited from amine-methanol mixtures possessed the exact composition of source bulk glass while thin films of other solvent formulations exhibited sulfur deficiency. The annealing up to 210 °C only highlighted this difference. As a result of the different structure of the thin films prepared in this way, the other benefits of methanol addition were found, namely an increase in the refractive index by approx. 0.1 independent of the annealing temperature, or a lower thermally induced thickness contraction (up to 7.5 %).

Sulfur Deficiency in Cotton: Causes, Symptoms, and Considerations

The purpose of this article is to provide information to cotton farmers, Extension agents, and crop consultants about the symptoms of sulfur deficiency, the various sources of sulfur, and its interaction with changing rates of other major nutrients such as nitrogen. Written by Hardeep Singh, Ethan Carter, Lakesh Sharma, Sudeep Sidhu, and Peter Omara, and published by the UF/IFAS Agronomy Department, November 2023.

MoS2−x/GCD-MoS2−x nanostructures for tuning the overpotential of Volmer-Heyrovsky reaction of electrocatalytic hydrogen evolution

MoS2 nanostructures are famous electrocatalysts in hydrogen evolution reaction (HER). The presence of graphene was already reported to boost the electrocatalytic activity of MoS2. This work reports the influence of Sulphur deficiency and economically synthesizable green carbon dot (GCD) incorporation into hydrothermally synthesized MoS2 nanostructures on the electrocatalytic activity of HER. All as-synthesized nanocatalysts are Sulphur deficient (MoS2−x and GCD-MoS2−x) and exhibited superior HER activity as compared to commercial MoS2. MoS2−x 220 exhibited a Tafel slope of 43 mV/Dec indicates the reaction mechanism as Volmer-Heyrovsky with Heyrovsky as rate determining step. Best electrocatalytic parameters are observed for the same catalyst with overpotential: 247 mV at 10 mA/cm2, Turnover frequency: 0.42 s−1, double layer capacitance: 23.8 mF/cm2 and electrochemically active surface area: 595/cm2. The obtained preferable HER parameters suggest that Sulphur deficiency and incorporation of GCD in MoS2 are playing a vital role in HER electrocatalysis.

Effect of Sulfur Deficiency on the Structural and Superstructural Features of Ga1.29In3.38S7

Effect of Sulfur Deficiency on the Structural and Superstructural Features of Ga1.29In3.38S7

Enhancing Soybean Yield: The Synergy of Sulfur and Rhizobia Inoculation.

Sulfur deficiency severely limits soybean growth, inhibiting the rhizobia nitrogenase and soybean protein synthesis. This study assessed the impact of sulfur fertilization and rhizobia inoculation on soybean growth and nitrogen fixation through bacterial culture and hydroponic experiments. We selected three rhizobia strains for bacterial cultures and used six sulfur levels. The test demonstrated severe inhibition of Rhizobium USDA110 growth without sulfur. In hydroponic experiment, we employed five sulfur levels with USDA110 as the inoculum strain. Soybean growth, nitrogen fixation, yield, and root morphology-related parameters, and root nodule growth, were significantly inhibited without sulfur. Following Rhizobium inoculation, low sulfur concentrations (0.15-0.60 mM) stimulated early-stage (V9) root growth and increased shoot nitrogen accumulation, but inhibited root growth at R5 stage. Furthermore, Rhizobium inoculation notably enhanced soybean growth, nitrogen fixation, and yield, especially within the recommended low sulfur concentration range (0.15-0.30 mM). The maximum nodule nitrogenase activity at R5 stage and highest yield was recorded at a 0.3 mM sulfur concentration with Rhizobium inoculation, which was 9.51-1222.07% higher than other treatments. These findings highlight that low sulfur concentration and rhizobia inoculation enhance soybean growth, nitrogen fixation, and yield but reduce soybean root efficacy, increasing reliance on root nodules.

Perennial Ryegrass (Lolium perenne L.) Response to Different Forms of Sulfur Fertilizers

The aim of the study was to compare sulfate fertilizers and mixtures of elemental sulfur (S0) and sulfate in terms of yield and nitrogen (N) and sulfur (S) status in perennial ryegrass. Mixtures of sulfate and S0 can reduce the consumption of sulfate alone. The plants were grown in soil cultures. The plants were supplemented with S0, K2SO4, MgSO4, and (NH4)SO4 or a mixture of these salts with So. Two sulfur doses were applied and the ryegrass was harvested three times. Fresh and dry weights of each swath, the N and S content, and their uptake were determined. The total fresh yield of sulfur-fertilized plants was 25 to 94% higher compared to unfertilized plants. The increases in dry matter were even more significant. Fertilizers, being a mixture of S0 and sulfate, showed the same efficiency as those containing sulfate alone. Sulfur fertilization resulted in a higher S content and its uptake, lowered N concentration in second and third swatch, and a decrease in total N uptake. In conclusion, to achieve high crop yields, soil sulfur deficiency should be corrected and fertilizers that are the mixture of elemental sulfur and sulfate are a beneficial and effective approach.

Overexpression of BoLSU1 and BoLSU2 Confers Tolerance to Sulfur Deficiency in Arabidopsis by Manipulating Glucosinolate Metabolism.

Sulfur is an essential element for plant growth, development and resistance to environmental stresses. Glucosinolates (GSLs), a group of sulfur rich secondary metabolites found in Brassicaceae plants, are known for their defensive properties against pathogens and herbivores. Due to their integration of a large proportion of total sulfur, their biosynthesis and degradation are closely linked to sulfur metabolism. It has been demonstrated that GSLs can be broken down to release sulfur and facilitate the production of other thio-metabolites when the plant is under stress. However, the regulation of this process is still not fully understood. In this study, we constructed two broccoli LSU (low sulfur responsive) gene overexpressing lines, 35S::BoLSU1 and 35S::BoLSU2, to detect changes in GSL metabolism after sulfur deficiency treatment. The results showed that BoLSU1 and BoLSU2 inhibit the biosynthesis of aliphatic GSLs, while also promoting their degradation and increasing the content of glutathione (GSH), leading to the reallocation of sulfur from the GSL pool to other thio-metabolites such as GSH. Furthermore, this regulation of GSL metabolism mediated by BoLSU1 and BoLSU2 is found to be dependent on myrosinases BGLU28 and BGLU30. Our study provides insight into the physiological role of LSU proteins and their regulation of sulfur metabolism.

Effect of sulfur fertilization on the composition, functionality, and protein quality of navy beans (Phaseolus vulgaris)

AbstractBackgroundSoil sulfur deficiency leads to reduced crop yield and quality loss, which is especially critical for pulses as sulfur is a major component of methionine and cysteine. The present research evaluated the influence of sulfur fertilization on two varieties of navy beans grown at soil sulfur levels of 17, 26, 35, and 44 kg/ha by analyzing their composition, functionality, and protein quality.ResultsIncreasing the soil application of sulfur did not affect the protein content of the test navy bean flours. Methionine, cysteine, and tryptophan were limiting, and their concentrations were not enhanced by sulfur fertilization nor was protein digestibility. The oil holding capacity and emulsion stability were different among sulfur treatments; however, no clear trend was observed.ConclusionSulfur fertilization had only a minimal effect on the quality attributes of navy beans, possibly due to a combined effect of environmental factors that limited the plant's response to sulfur supply. Despite the limited change, the findings underlined the possible role of the environment (e.g., location, crop year, and other variables) in determining the sulfur fertilization response of navy beans and provided valuable insight for researchers to further investigate the topic and optimize navy bean production and quality.

Optimizing antimony trisulfide thin films: enhanced structural and optoelectronic properties via post-annealing in sulfur atmosphere

Antimony trisulfide (Sb2S3) is a promising material for photoabsorption due to its high absorption coefficient, low toxicity, and abundance in nature. However, various stoichiometric and crystalline defects have limited its practical applications. Herein, highly crystalline Sb2S3 thin films are fabricated via thermal evaporation and subsequent annealing in a sulfur atmosphere at different temperatures. The analyses of X-ray diffraction, Raman spectroscopy, scanning electron microscopy, and optical properties reveal highly crystalline Sb2S3 thin films. The films showed reduced stoichiometric and crystal defects, while also displyaing enhanced optical and morphological properties. The improvement in the physical properties of the Sb2S3 thin films is attributed to the filling of sulfur deficiencies by extra sulfur vapors present during the thermal annealing process. Moreover, annealing the obtained Sb2S3 samples at different temperatures (ranging from 250 °C to 300 °C) reveals that the Sb2S3 films exhibit improved physical properties at 300 °C, which is considered to be the optimal annealing temperature. Our findings open up a pathway for the fabrication of highly crystalline Sb2S3 thin films, which could lead to the development of efficient optoelectronic devices.

The effect of glutathione on glucosinolate biosynthesis through the sulfur assimilation pathway in pakchoi associated with the growth conditions

Glucosinolates (GSLs) are a group of nitrogen- and sulfur-containing secondary metabolites, synthesized primarily in members of the Brassicaceae family, that play an important role in food flavor, plant antimicrobial activity, resistance to insect attack, stress tolerance, and human anti-cancer effects. As a sulfur-containing compound, glutathione has a strong connection with GSLs biosynthesis as a sulfur donor or redox system, and exists in reduced (glutathione; GSH) and oxidized (glutathione disulfide; GSSG) forms. However, the mechanism of GSH regulating GSLs biosynthesis remainds unclear. Hence, the exogenous therapy to pakchoi under normal growth condition and sulfur deficiency condition were conducted in this work to explore the relevant mechanism. The results showed that exogenous application of buthionine sulfoximine, an inhibitor of GSH synthesis, decreased the transcript levels of GSLs synthesis-related genes and transcription factors, as well as sulfur assimilation-related genes under the normal growth condition. Application of exogenous GSH inhibited the expression of GSLs synthesis- and sulfur assimilation-related genes under the normal condition, while the GSLs biosynthesis and the sulfur assimilation pathway were activated by exogenous application of GSH when the content of GSH in vivo of plants decreased owing to sulfur deficiency. Moreover, exogenous application of GSSG increased the transcript levels of GSLs synthesis- and sulfur assimilation-related genes under the normal growth condition and under sulfur deficiency. The present work provides new insights into the molecular mechanisms of GSLs biosynthesis underlying glutathione regulation.

Methionine restriction-induced sulfur deficiency impairs antitumour immunity partially through gut microbiota

Restriction of methionine (MR), a sulfur-containing essential amino acid, has been reported to repress cancer growth and improve therapeutic responses in several preclinical settings. However, how MR impacts cancer progression in the context of the intact immune system is unknown. Here we report that while inhibiting cancer growth in immunocompromised mice, MR reduces T cell abundance, exacerbates tumour growth and impairs tumour response to immunotherapy in immunocompetent male and female mice. Mechanistically, MR reduces microbial production of hydrogen sulfide, which is critical for immune cell survival/activation. Dietary supplementation of a hydrogen sulfide donor or a precursor, or methionine, stimulates antitumour immunity and suppresses tumour progression. Our findings reveal an unexpected negative interaction between MR, sulfur deficiency and antitumour immunity and further uncover a vital role of gut microbiota in mediating this interaction. Our study suggests that any possible anticancer benefits of MR require careful consideration of both the microbiota and the immune system.

Sulfur fertilization effects on protein concentration and yield of wheat: A meta-analysis

Wheat (Triticum aestivum L.) has a low sulfur (S) requirement compared with other macronutrients, such as nitrogen (N) and potassium (K), and similar to that of magnesium (Mg), phosphorus (P) or calcium (Ca). However, a lack of this nutrient can affect yield and protein concentration and composition, determining the characteristics of the flour. Sulfur deficiency in cropping systems is increasingly reported worldwide, but the effects of S fertilizer are inconsistent among studies depending upon genotype, environment, and management factors. Therefore, our goals were to analyze the results from published studies through a meta-analysis to quantify the effect of S fertilizer on protein concentration and yield of wheat, and to identify environmental, genotypic and management factors behind the response of protein concentration and yield to S fertilizer. This meta-analysis summarizes 22 studies reporting protein concentration, from which 15 also reported yield, most of them published in the 2000’s. We calculated the effect size as the natural log of the response ratio, with the response ratio determined as protein concentration (or yield) with S fertilizer application relative to protein concentration (or yield) without S fertilizer application. On average, S fertilizer increased protein concentration by 5.4 % and yield by 16.2 %. Sulfur fertilizer impact on protein concentration and yield increased with the relatively low soil S levels of our dataset (under 7.20 mg S kg-1), under wet weather conditions (above historical precipitation average), no-till practices, or following legumes. Furthermore, ensuring a balanced nutrition by the co-application of N was a determining factor in achieving a response to S fertilizer, which increased with higher N rates. Interestingly, we found a consistent positive response to S fertilizer for yield among all bread-making quality categories, and only a positive response for protein in high and low breadmaking quality, but not in the medium quality category. Overall, this meta-analysis provides an extensive evaluation of protein and yield response to S fertilization in wheat, and how these responses are influenced by genotypic, environmental, and management factors.

Chemical homogeneity and sulfur deficiency in the early B-type stars of the λ Orionis group

ABSTRACT We present a detailed abundance analysis of nine early B-type stars in the λ Orionis group. High- and medium-resolution spectra of the target stars were observed at the Ankara University Kreiken Observatory or acquired from the European Southern Observatory and Observatoire de Haute-Provence archives. The atmospheric parameters of each star were estimated from the hydrogen Balmer, ionized silicone, and helium lines. Non-LTE model atmosphere calculations determined the precise abundances of helium, carbon, nitrogen, oxygen, neon, magnesium, silicon, sulfur, and iron from the spectra of each target star. While the abundances of the light elements He, C, N, O, Ne, Si, and also Fe are close to solar, Mg, Al, and S are deficient, and Ar is overabundant compared to the Sun. The phosphorus absorption lines were only accessible on the optical spectra of HD 43112, HD 34989, 37 Ori, and HD 36895. The phosphorus mean abundance values of these four stars are underabundant compared to the Sun. Argon was only detected on the spectra of HD 43112 and HD 36262 and showed an overabundance of 0.33 dex. The sulfur element deficiency was detected in all stars except for 35 Orionis. Sulfur may be a good signature to distinguish the λ Orionis group members from the Orion star-forming complex. Overall, chemical homogeneity was detected in all B-type star members of the λ Orionis group. Furthermore, the mass and age of each member were calculated and plotted on the H–R diagram.

Vertical Distribution of Forms of Sulphur in Relation to Physicochemical Properties of Lateritic Soils of Eastern India

Sulphur (S) deficiency is widespread in Indian soils, and it has been emerging as a major problem in rice-growing lateritic soils of West Bengal. The vertical distribution of S forms and their relationships with soil physicochemical properties were examined in 50 rice-growing locations of lateritic soils of West Bengal, India, for the current study. For this a total of one hundred fifty representative soil samples were collected from three depths and fifty representative locations of rice-based cropping systems of lateritic belt of West Bengal and analyzed for different fractions of S and important physicochemical properties using standard methodology. Most soils were sandy clay loam to clay loam, with low to medium levels of organic carbon, and strongly acidic to moderately acidic. A decrease in organic carbon across the depths was evident. Among the sulphur forms, organic S was dominant, and the contribution of water-soluble S was least throughout the soil profile. The studied sulphur forms followed the order: total S > organic S > heat soluble S > adsorbed S > sulphate S > water soluble S. Except for adsorbed S, most forms decreased with soil depth. The correlation study revealed that soil pH was positively and significantly correlated with water soluble (r = 0.431**) and sulphate S (r = 0.325*) in surface soils, with sulphate S both in mid-surface (r = 0.450**) and subsurface soils (r = 0.423**). Organic carbon showed a positive correlation with all the S forms throughout the profile. Both clay and silt content positively affected S forms, while sand content showed a negative relationship. Positive correlation among all the S forms observed along soil depth, except for a negative correlation between adsorbed S and sulphate S. This investigation will be helpful for sulphur management to optimize crop yields in lateritic soils.

Effects of Sulfate on the Physiology, Biochemistry, and Activity of Group 1 Sulfate Transporters in Seedlings of Brassica pekinensis

It is well known that some plants have the capability of taking up sulfur as a nutrient from the atmosphere through foliar absorption and can survive well in polluted environments. In order to observe the effects of the relationship between atmospheric hydrogen sulfide (H2S) deposition and soil sulfur nutrition, the current study used Brassica pekinensis as a model plant. The objective in conducting this study was to understand the regulatory mechanisms engaged in the uptake and assimilation of sulfate (SO42−) in plants by studying the modulation of transcription levels of sulfate transporter genes (STGs) (Sultr1;1 and Sultr1;2), changes in growth physiology, and the potential of roots to uptake the SO42− when allowed to grow in the presence or absence of SO42− in a hydroponic nutrient solution. Changes in growth, physico-chemical parameters, and gene expression levels of Group 1 STGs were observed when sulfur-treated and non-treated plants were exposed to phytotoxic H2S levels in the air. Sulfur deficiency enhanced nitrate and free amino acid (FAA) concentrations in the shoot and root regions of the plant. However, there was a significant decrease in the biomass, shoot/root ratio (SRR), chlorophyll content, and thiol content, with p-values < 0.01. This, in turn, increased the sulfur-uptake capacity of plants from the atmosphere through foliar absorption. When the sulfur-uptake capacity of plants increased, there was an increase in the expression level of Group 1 sulfate transporter genes (Sultr1;1 and Sultr1;2), which regulate sulfur transportation through roots. The growth, physico-chemical characteristics, and level of gene expression of Group 1 STGs were unaffected by the availability of excess sulfur in the atmosphere of up to 0.3 μL l−1.