Sulfur Deficiency Research Articles

Pulses as a Key Component in Conservation Agriculture: Impacts on Soil Health and Sustainability

Intensive agriculture poses several challenges such as soil degradation, reduced soil organic matter, and increased soil nutrient imbalances including nitrogen, phosphorus, potassium, sulfur, zinc, iron, and manganese deficiencies and nutrient toxicities (Tiwari KN, 2002). It also contributes to degradation of groundwater quality, declining groundwater levels (Humphreys E et al, 2010), and issues like sodicity and salinization (Tiwari VM et al, 2009), resulting in reduced crop productivity. Many of these limitations can be addressed by adopting conservation agriculture, which supports sustainable productivity while safeguarding natural resources (Sharma AR et al, 2012). Pulses are particularly beneficial in conservation agriculture as they align well with its principles: diversified crop rotations, minimum soil disturbance and continuous soil cover. They serve as a resource-conserving technology that mitigates the adverse impacts of modern agricultural practices.

Quantitative Proteomic Analysis of Brassica Napus Reveals Intersections Between Nutrient Deficiency Responses.

Macronutrients such as nitrogen (N), phosphorus (P), potassium (K) and sulphur (S) are critical for plant growth and development. Field-grown canola (Brassica napus L.) is supplemented with fertilizers to maximize plant productivity, while deficiency in these nutrients can cause significant yield loss. A holistic understanding of the interplay between these nutrient deficiency responses in a single study and canola cultivar is thus far lacking, hindering efforts to increase the nutrient use efficiency of this important oil seed crop. To address this, we performed a comparative quantitative proteomic analysis of both shoot and root tissue harvested from soil-grown canola plants experiencing either nitrogen, phosphorus, potassium or sulphur deficiency. Our data provide critically needed insights into the shared and distinct molecular responses to macronutrient deficiencies in canola. Importantly, we find more conserved responses to the four different nutrient deficiencies in canola roots, with more distinct proteome changes in aboveground tissue. Our results establish a foundation for a more comprehensive understanding of the shared and distinct nutrient deficiency response mechanisms of canola plants and pave the way for future breeding efforts.

Impact of Sulfur Deficiency and Excess on the Growth and Development of Soybean Seedlings.

Sulfur is a critical element for plant growth and development, serving as a component of amino acids (cysteine and methionine), iron-sulfur clusters, proteins, glutathione, coenzymes, and auxin precursors. Deficiency or low concentrations of sulfur in the soil can lead to significant growth retardation in plants. The objective of our study was to examine the effects of sulfur (S) deficiency and excess on morphological symptoms, sulfur and nitrogen (N) metabolism, as well as antioxidant activity in soybean. We found that S starvation decreased the fine root length, biomass, and activity, and the chlorophyll content was reduced, while excess sulfur promotes lateral root growth. In contrast to sulfur excess, sulfur deficiency inhibits N and S metabolism levels in both subsurface and above-ground parts, and induced the expression of some sulfur transporters (SULTRs). In this study, we created soybean hairy root lines overexpressing the SULTR gene (GmSULTR2;1a) to observe metabolic changes following sulfur deficiency treatment. The results showed that GmSULTR2;1a saved the sulfur-deficient phenotype, and the antioxidant enzyme activity was much higher than that of the wildtype in the absence of sulfur. Our study revealed the important role of sulfur element in soybean growth and development and the regulation of sulfur deficiency by GmSULTR2;1a.

Sulphur Fractions, Distribution, and Connections with Soil Attributes in the Gopalpur and Ishwardi Soil Series of Bangladesh

The deficiency of soil sulfur (S) is a prevalent issue in Bangladesh soils, posing a significant obstacle to enhancing crop yield and overall productivity. This study aimed to evaluate the distribution of different sulfur fractions across various soil depths in the Gopalpur and Ishwardi soil series, along with their relationships with other soil parameters. In both soil series, the sulfur fractions were ordered as follows: organic > adsorbed > available forms, with organic sulfur being the most abundant. Additionally, soil attributes such as pH, organic carbon, texture, and EC were assessed to understand their relationships with sulphur fractions. Results indicate significant variations in sulphur distribution between the two soils series, with organic sulphur dominating in Gopalpur soils and sulphate-sulphur prevailing in Ishwardi soils. Correlation analysis reveals strong associations between sulphur fractions and soil attributes, highlighting the influence of soil properties on sulphur dynamics. These findings provide valuable insights into sulphur cycling in agricultural soils of Bangladesh and underscore the importance of soil management practices for optimizing sulphur availability and crop productivity.

EF yolov8s: A Human–Computer Collaborative Sugarcane Disease Detection Model in Complex Environment

The precise identification of disease traits in the complex sugarcane planting environment not only effectively prevents the spread and outbreak of common diseases but also allows for the real-time monitoring of nutrient deficiency syndrome at the top of sugarcane, facilitating the supplementation of relevant nutrients to ensure sugarcane quality and yield. This paper proposes a human–machine collaborative sugarcane disease detection method in complex environments. Initially, data on five common sugarcane diseases—brown stripe, rust, ring spot, brown spot, and red rot—as well as two nutrient deficiency conditions—sulfur deficiency and phosphorus deficiency—were collected, totaling 11,364 images and 10 high-definition videos captured by a 4K drone. The data sets were augmented threefold using techniques such as flipping and gamma adjustment to construct a disease data set. Building upon the YOLOv8 framework, the EMA attention mechanism and Focal loss function were added to optimize the model, addressing the complex backgrounds and imbalanced positive and negative samples present in the sugarcane data set. Disease detection models EF-yolov8s, EF-yolov8m, EF-yolov8n, EF-yolov7, and EF-yolov5n were constructed and compared. Subsequently, five basic instance segmentation models of YOLOv8 were used for comparative analysis, validated using nutrient deficiency condition videos, and a human–machine integrated detection model for nutrient deficiency symptoms at the top of sugarcane was constructed. The experimental results demonstrate that our improved EF-yolov8s model outperforms other models, achieving mAP_0.5, precision, recall, and F1 scores of 89.70%, 88.70%, 86.00%, and 88.00%, respectively, highlighting the effectiveness of EF-yolov8s for sugarcane disease detection. Additionally, yolov8s-seg achieves an average precision of 80.30% with a smaller number of parameters, outperforming other models by 5.2%, 1.9%, 2.02%, and 0.92% in terms of mAP_0.5, respectively, effectively detecting nutrient deficiency symptoms and addressing the challenges of sugarcane growth monitoring and disease detection in complex environments using computer vision technology.

An EIL Family Transcription Factor From Switchgrass Affects Sulphur Assimilation and Root Development in Arabidopsis.

Sulphur limitation 1 (SLIM1), a member of ethylene-insensitive3-like (EIN3/EIL) protein family, is recognised as the pivotal transcription factor regulating sulphur assimilation, essential for maintaining sulphur homoeostasis in Arabidopsis. However, the function of its monocot homologues is largely unknown. In this study, we identified PvEIL3a, a homologous gene of AtSLIM1, from switchgrass (Panicum virgatum L.), a significant perennial bioenergy crop. Our results demonstrated that introducing PvEIL3a into Arabidopsis slim1 mutants significantly increased the expression of genes responsive to sulphur deficiency, and transgenic plants exhibited shortened root length and delayed development. Moreover, PvEIL3a activated the expression of AtAPR1, AtSULTR1;1 and AtBGLU30, which plays an important role in sulphur assimilation and glucosinolate metabolism. Results of transcriptome and metabonomic analysis further indicated a perturbation in the metabolic pathways of tryptophan-dependent indole glucosinolates (IGs), camalexin and auxin. In addition, PvEIL3a conservatively regulated sulphur assimilation and the biosynthesis of tryptophan pathway-derived secondary metabolites, which reduced the biosynthesis of indole-3-acetic acid (IAA) and inhibited the root elongation of transgenic Arabidopsis. In conclusion, this study highlights the functional difference of the ethylene-insensitive 3-like (EIL) family gene in monocot and dicot plants, thereby deepening the understanding of the specific biological roles of EIL3 in monocot plant species.

Identification and Expression Analysis of Sulfate Transporter Genes Family and Function Analysis of GmSULTR3;1a from Soybean.

Sulfate transporters (SULTRs) are essential for the transport and absorption of sulfate in plants and serve as critical transport proteins within the sulfur metabolism pathway, significantly influencing plant growth, development, and stress adaptation. A bioinformatics analysis of SULTR genes in soybean was performed, resulting in the identification and classification of twenty-eight putative GmSULTRs into four distinct groups. In this study, the characteristics of the 28 GmSULTR genes, including those involved in collinearity, gene structure, protein motifs, cis-elements, tissue expression patterns, and the response to abiotic stress and plant hormone treatments, were systematically analyzed. This study focused on conducting a preliminary functional analysis of the GmSULTR3;1a gene, wherein a high expression level of GmSULTR3;1a in the roots, stems, and leaves was induced by a sulfur deficiency and GmSULTR3;1a improved the salt tolerance. A further functional characterization revealed that GmSULTR3;1a-overexpressing soybean hairy roots had higher SO42-, GSH, and methionine (Met) contents compared with the wild-type (WT) plant. These results demonstrate that the overexpression of GmSULTR3;1a may promote the sulfur assimilation metabolism and increase the content of sulfur-containing amino acids in plants.

Effects of soil and phylogeny on sulfur and phosphorus concentrations in wild plants on volcanic and non-volcanic soils in Japan

ABSTRACT Andosols are soils characterized by relatively high sulfur concentrations, but also by their low phosphorus availability for crops and vegetables. We assessed the effect of soil type on the concentrations of sulfur and phosphorus in plants using handheld X-ray fluorescence (XRF) analysis on herbarium specimens collected from Andosols and non-volcanic soils in Japan. The sulfur concentrations in plants from Andosols were higher than plants from non-volcanic soils, but there was no significant difference between their foliar phosphorus concentrations. Only three specimens out of 1203 herbarium specimens were below the sulfur concentration (1000 mg kg−1) needed for adequate growth of crops, but about half of specimens were below that for phosphorus (2000 mg kg−1). The sulfur concentrations were high in herbaceous plants, especially in plants from the Brassicaceae and Liliaceae families. Overall, the results show that the sulfur concentrations in wild plants from Japan were higher in Andosols, with a low risk for sulfur deficiency. In contrast, the effect of soil type on the phosphorus concentrations in plants was small, and this may be explained because most wild plants grow under phosphorus-limiting conditions. Finally, the analysis reveals a strong correlation between sulfur and phosphorus concentrations in plants, suggesting the existence of a mechanism that controls the sulfur and phosphorus concentration ratios.

Chalcogenide perovskite BaZrS3 bulks for thermoelectric conversion with ultra-high carrier mobility and low thermal conductivity

Chalcogenide perovskites are expected to be promising thermoelectric materials, since they not only possess efficient carrier transport and defect tolerance, but also demonstrate unique advantages of high thermodynamic stability, eco-friendly and earth-abundant constituents. Especially, theoretical reports have predicted their “glass-like” thermal conductivities. However, experimental investigation on thermoelectric performances of chalcogenide perovskite BaZrS3 is extremely scarce due to the difficulty in preparing high-quality bulk samples, which originates from the brittle nature, high melting point, and the large difference in melting points between Ba/S and Zr. In this work, pure phase BaZrS3 bulks with high relative density reaching 100 % are realized by optimized sulfurization from low-cost BaZrO3 powders combined with fast spark plasma sintering. A maximum zT value of 0.37 at 623 K in BaZrS3 bulks is achieved, which is the record-high value among the reported sulfide, halide, and hybrid perovskite materials. A room-temperature electron mobility up to 385 cm2V−1s−1 is among the highest values for perovskites due to the high phase purity, dense morphology and corner-sharing ZrS6 octahedral three-dimensional network as effective carrier channels. Meanwhile, a measured low lattice thermal conductivity of 1.11 Wm−1K−1 at 623 K is attributed to the intense phonon scattering from the intrinsic distorted-perovskite structure and the lattice defects by sulfur deficiency. Moreover, the BaZrS3 bulks in this work are stable against moisture/air and high temperature test. This work provides new insights into the fundamental electrical and thermal properties of chalcogenide perovskites, and highlights their great potential in the practical thermoelectric applications.

The Effect of Manganese and Sulfur Applications on the Rocket (Eruca vesicaria subsp. Sativa) Plant Grown in Lime Soils

Due to the high lime and pH in the soil, sulfur and micro element deficiencies are observed in green vegetable plants. Rocket, one of the green vegetables and a member of the Brassicaceae family, is widely produced in our country. It is noteworthy that rocket is grown by producers almost everywhere and that this plant grown in our region also has a deficiency of microelements. For this reason, in this study were investigated that the sulfur and manganese requirement of rocket, which is one of the vegetable plants grown in Konya calcareous soil, and the effect of the interaction between these two elements on plant growth and yield. In our study, sulfur (S) fertilizer was applied in three doses (0- 40- 80 kg S da-1) and manganese (Mn) fertilizer in three doses (0- 1- 2 kg Mn da-1) was applied to the rocket plant. The experiment was carried out in the greenhouse conditions according to the randomized plots factorial design. At the end of the experiment, the average leaf height, chlorophyll SPAD value, plant fresh weight, plant height and plant Mn concentration did not differ with different doses of fertilizer applications in the rocket plant. On the other hand, the sulfur concentration in the plant and soil increased significantly with sulfur fertilization (p

The impact of sulfur deficiency on the structural, optical and photoluminescence properties of Zn0.75Cd0.25S quantum dots

The impact of sulfur deficiency on the structural, optical and photoluminescence properties of Zn0.75Cd0.25S quantum dots

Влияние новых видов обогащенных удобрений на продуктивность культур и агрохимические свойства почв

The effect and aftereffect of new types of enriched sulfur-containing fertilizers on the productivity of spring wheat of the Novosibirskaya 31 variety and peas of the Radomir variety, as well as the agrochemical properties of leached chernozem and agro-gray soil of the Krasnoyarsk forest-steppe, characterized by an acute deficiency of mobile sulfur, were studied. It was found that the application of fertilizers containing sulfur statistically significantly increased the increase in productivity of pea biomass in the year of application. With the aftereffect of spring wheat, an increase in productivity was noted on leached chernozem; the aftereffect of fertilizers for wheat on agro-gray soil was not effective. The application of enriched fertilizers did not affect the acid-base balance of soils. In chernozem leached in all fertilized variants, the supply of mineral forms of nitrogen is high, while the content of mobile sulfur increases. The supply of nitrate and ammonium forms of nitrogen in agro-gray soil is low, and the amount of mobile sulfur does not increase. Keywords: SULFUR, ENRICHED FERTILIZERS, SPRING WHEAT, PEAS, AGROCHEMICAL INDICATORS, PRODUCTIVITY, AGROGY SOIL, LEACHED CHERNOZEM

The impact of benzoic acid and lactic acid on the treatment efficiency and microbial community in the sulfur autotrophic denitrification process.

Nitrate poses a potential threat to aquatic ecosystems. This study focuses on the sulfur autotrophic denitrification mechanism in the process of water culture wastewater treatment, which has been successfully applied to the degradation of nitrogen in water culture farm effluents. However, the coexistence of organic acids in the treatment process is a common environmental challenge, significantly affecting the activity of denitrifying bacteria. This paper aims to explore the effects of adding benzoic acid and lactic acid on denitrification performance, organic acid removal rate, and microbial population abundance in sulfur autotrophic denitrification systems under optimal operating conditions, sulfur deficiency, and high hydraulic load. In experiments with 50 mg·L-1 of benzoic acid or lactic acid alone, the results show that benzoic acid and lactic acid have a stimulating effect on denitrification activity, with the stimulating effect significantly greater than the inhibitory effect. Under optimal operating conditions, the average denitrification rate of the system remained above 99%; under S/N = 1.5 conditions, the average denitrification rate increased from 88.34% to 91.93% and 85.91%; under HRT = 6h conditions, the average denitrification rate increased from 75.25% to 97.79% and 96.58%. In addition, the addition of organic acids led to a decrease in microbial population abundance. At the phylum level, Proteobacteria has always been the dominant bacterial genus, and its relative abundance significantly increased after the addition of benzoic acid, from 40.2% to 61.5% and 62.4%. At the genus level, Thiobacillus, Sulfurimonas, Chryseobacterium, and Thermomonas maintained high population abundances under different conditions. PRACTITIONER POINTS: Employing autotrophic denitrification process for treating high-nitrate wastewater. Utilizing organic acids as external carbon sources. Denitrifying bacteria demonstrate high utilization efficiency towards organic acids. Organic acids promote denitrification more than they inhibit it. The promotion is manifested in the enhancement of activity and microbial abundance.

Improving the seed yield and quality of canola by using sulfur, strigolactone, and growth promoting microorganisms

The canola needs more sulfur fertilizer than cereals to reach maximum yield. Also, strigolactones can create an extensive root system in canola in order to raise the mineral and water absorption in conditions of deficiency of these substances and strigolactones levels increased under sulfur deficiency. This study was conducted to evaluate the reaction of canola to the application of sulfur, strigolactone and growth promoting microorganisms. Results showed that sulfur application increased seed yield by 23.22% and improved seed oil content by 2.27%. The effect of microorganisms on all traits was significant, especially seed yield enhanced by 3–12%. Thiobacillus had the most positive effect on seed yield and seed quality properties. Strigolactone had no significant effect on seed yield, although it slightly improved some traits. The inoculation of canola with Thiobacillus combined with sulfur and strigolactones increased seed yield by 47% and improve seed quality traits, so it can be recommended.

Regulation of nitrogen metabolism by COE2 under low sulfur stress in Arabidopsis

The interplay between nitrogen and sulfur assimilation synergistically supports and sustains plant growth and development, operating in tandem to ensure coordinated and optimal outcomes. Previously, we characterized Arabidopsis CHLOROPHYLL A/B-BINDING (CAB) overexpression 2 (COE2) mutant, which has a mutation in the NITRIC OXIDE-ASSOCIATED (NOA1) gene and exhibits deficiency in root growth under low nitrogen (LN) stress. This study found that the growth suppression in roots and shoots in coe2 correlates with decreased sensitivity to low sulfur stress treatment compared to the wild-type. Therefore, we examined the regulatory role of COE2 in nitrogen and sulfur interaction by assessing the expression of nitrogen metabolism-related genes in coe2 seedlings under low sulfur stress. Despite the notable upregulation of nitrate reductase genes (NIA1 and NIA2), there was a considerable reduction in nitrogen uptake and utilization, resulting in a substantial growth penalty. Moreover, the elevated expression of miR396 perhaps complemented growth stunting by selectively targeting and curtailing the expression levels of GROWTH REGULATING FACTOR 2 (GRF2), GRF4, and GRF9. This study underscores the vital role of COE2-mediated nitrogen signaling in facilitating seedling growth under sulfur deficiency stress.

Assessment of Soil Health and Soil Quality of Bokaro, District, Jharkhand, India

Taking care of soil health is most importance for sustainable crop production. Soil quality is a blending of soil processes and provides to a measure change in soil condition as related to following factors such as land use, climate patterns, cropping sequences and farming systems. In the present investigation analysis of different physical and chemical characteristics were made to determine soil health of Bokaro district. Results show that soil bulk density was in normal range, soil pore space % varied from 39.85 to 48.32 g cm-3, particle density ranged from 2.24 to 2.69 Mg m-3, water holding capacity varied from 34.23 to 43.86 %. Soil pH and EC were found to be acidic and non-saline in nature respectively while soil organic carbon and available phosphorus were in medium to high range. Available nitrogen and potassium were found to be low to medium range i.e., 197.0 to 219.0 Kg ha - 1 and 201.96 to 266.01 Kg ha -1 and the range of phosphorus varied from 16.96 to 26.68 kg ha -1 respectively. Deficiency of sulphur followed by zinc was found in soils of Bokaro district.

Growth Attributes of Maize (Zea Mays L) Crop and Nutrient Uptake as impacted by Sulphur and Micronutrients Application

Maize, also known as the "queen of cereals," is a highly nutritious crop that requires a significant amount of nutrients to grow. It is particularly sensitive to deficiency of sulphur. It plays a significant role in the synthesis of chlorophyll, oil, and protein. A field experiment was conducted at Rashtriya Chemicals and Fertilizers Limited in Mumbai, Maharashtra, during the Kharif season 2023, to assess the impact of elemental sulphur application and foliar spray of multi micronutrient on maize plant growth and uptake of nutrients by maize as well as the post-harvest status of soil. The experiment was laid out in Randomized Block Design (RBD) with five treatments that were replicated six times each. T1 is the absolute control, T2 is 100% RDF, T3 is the RDF NPK with 45 kg/ha of elemental S, T4 is the RDF NPK with 45 kg/ha coupled with foliar spray of multi micronutrient, and T5 is the RDF NPK with foliar spray of multi micronutrient. The results indicated that application of 45 kg of S per hectare, in combination with a foliar spray of multi micronutrients, resulted in remarkable growth parameters of maize crops. These included plant height, cob height, yield (in kg/ha), dry weight per plant and also the uptake of essential nutrients. This was markedly higher when compared to the other treatments. Except 100 grain weight which was at par with other treatments. While girth of cob and protein content were found non-significant. The study reports suggest that the combined use of S and multimicronutrient in maize crop positively impact the uptake of nutrients and growth attributes.

Sulfur dynamics in saline sodic soils: The role of paddy cultivation and organic amendments

The phenomenon of sulfur (S) deficiency in Chinese soil is becoming increasingly severe, especially in the northeastern saline-sodic wasteland (WL). To determine the bioavailability of S in long-term cultivated paddy soil with or without organic amendment addition, the long-term field experiment site was established and incubation experiments were conducted. Compared to WL, soil properties have been continuously improved during the 15-year paddy field (PF) cultivation, ultimately enhancing the content of soil organic carbon (by 242.62 %), total nitrogen (by 126.49 %) and alkali-hydrolyzed nitrogen (by 28.07 %). Additionally, soil salinization has been decreasing, and the stock of total sulfur (TS) and available sulfur (AS) in the 0–60 cm soil layer have increased by 32.09 % and 293.52 % respectively. In contrast, organically-amended soil (OA) manifested superior TS and AS levels vs. PF. Additionally, water-soluble sulfur (WSS) was markedly elevated, registering post-annual increments of 227.31 % and 268.36 % in PF and OA, respectively, relative to WL. The key mechanistic impetus for the application of organic amendments was the enhancement of the soil's adsorptive and mineralization capabilities. As observed in our soil culture experiment, the maximum adsorption of sulfate ions and the cumulative mineralization of sulfur in the soil follow the pattern of OA > PF > WL. The Partial Least Squares Path Model (PLSPM) corroborated that both organic amendments and years of cultivation indirectly influenced total inorganic sulfur fractions. This influence is primarily mediated via structural soil enhancements and salinization amelioration, thereby elevating available quantities of sulfur in saline-sodic soil environments. In conclusion, from the perspective of soil sulfur biogeochemical cycling, we have confirmed that reclaiming saline-sodic wasteland for paddy field could be a beneficial and sustainable approach for soil management. These findings of this study can also provide an in-depth understanding of the bioavailability and influencing mechanism of sulfur in long-term saline-sodic paddy fields.

Role of Sulphur Nutrition in Oilseed Crops: A Review

India is a significant importer of edible oils and an important producer of oilseed. India stands as fourth leading oilseeds producing countries after USA, China and Brazil. Oilseed has become one of the most valuable agricultural commodities. Sulphur (S) plays an important role in determining the quality of oil seed from these crops. It is referred to as the fourth major nutrient after N, P and K because crops need sulphur slightly less than they require P. It is required for the formation of proteins, vitamins and chlorophyll. It is an integral part of amino acid such as cystine, cysteine and methionine which are essential components of protein. Plant can absorb sulphur in the form of sulphate (SO4 2-) which is decomposes into sulphur. It is a factor for the determination of higher yield seed and quality of seed that content oil in oilseed crops. Since, sulphur is immobile in plants, it must be continuously supplied from crop emergence till maturity. So, reduced yield can come sulphur shortage at any stage of growth. It can be overcome by supplying sulphur containing fertilizers to plant by soil application and foliar application. Among the source of sulphur, elemental sulphur and gypsum are widely utilized in sulphur deficiency.

Agricultural limitations to soil carbon sequestration: Plant growth, microbial activity, and carbon stabilization

Soil carbon (C) sequestration is an important process for mitigating climate change while improving soil productivity. At the same time, it presents challenges for nutrient availability and changing agricultural practices. The aim of this study was to survey the challenges and limitations found on pioneering farms testing C sequestration using a Finnish network of farmers testing carbon farming practices. A combination of satellite monitoring, on-site measurements and soil analysis was used to quantify and evaluate soil physical, chemical, and biological quality indicators and plant productivity on 20 farms (40 fields). The indicators were assessed through a conceptual C sequestration model, classifying them into limitations for three stages of plant growth, microbial activity or C stabilization. Based on the results, there are limitations in all three stages: plant growth is constrained by crop choices which limit leaf area and sunlight capture, microbial processing of C is limited by poor soil structure and moisture stress, and C stabilization is limited by waterlogging and sulfur deficiency. These problems were found to be widespread, but not present on all the farms. Bringing all the farms to the levels of the best performing farms could reduce the considerable gap between potential productivity and C sequestration and that achieved in practice.