Sulfated Polysaccharide Fractions Research Articles

Antioxidant activities of sulfated polysaccharide fractions from Porphyra haitanesis

Three sulfated polysaccharide fractions (F1, F2, and F3) were isolated from Porphyra haitanesis, an important economic alga in China, through anion-exchange column chromatography and their in vitro antioxidant activities were investigated in this study. Galactose was the main sugar unit of the three fractions. The analytical results indicated that polysaccharide fractions from P. haitanesis had similar chemical components to porphyran from other species, but differed in their high sulfate content. The sulfate content of F1, F2 and F3 was 17.4%, 20.5% and 33.5% respectively. All three polysaccharide fractions showed antioxidant activities. They had strong scavenging effect on superoxide radical, and much weaker effect on hydroxyl free radical. Lipid peroxide in rat liver microsome was significantly inhibited, and H2O2 induced hemolysis of rat erythrocyte was partly inhibited by F1, F2 and F3. Among them, F3 showed strongest scavenging effect on superoxide radical; F2 had strongest effect on hydroxyl radical and lipid peroxide.

Isolation and partial characterization of a novel β- d-galactan sulfate from the brown seaweed Laminaria angustata var. longissima

The fastest-moving component (LA-5) on electrophoresis was isolated from a sulfated polysaccharide fraction of the brown seaweed Laminaria angustata var. longissima by anion-exchange chromatography, fractional precipitation with ethanol, and gel filtration. LA-5 consisted of galactose, fucose, glucuronic acid and sulfate (100:2:3:74) and had an apparent average molecular weight of 400 000. The results of enzymatic digestion, optical rotation and 13C-NMR suggested that galactose is present predominantly as the β- d-form in LA-5. By IR, 13C-NMR and methylation analyses, LA-5 has been shown to have a backbone of →3)-β- d-galactosyl-(1→6)-β- d-galactosyl-(1→. Most sulfate groups may be attached to position 6 of 3-linked galactosyl residues. This indicates that the polysaccharide is a new type of galactan sulfate different from the well known galactans from red seaweeds and ascidians. This is the first report on the presence of galactan sulfate in the brown seaweeds.

Isolation and preliminary characterization of fucose-containing sulfated polysaccharides with blood-anticoagulant activity from the brown seaweed Hizikia fusiforme

We reported that a crude, sulfated polysaccharide (SPS) fraction from Hizikia fusiforme showed considerable anticoagulant activity. In order to identify the biologically active substances, we attempted to isolate each of the polysaccharide components from the SPS fraction. As a result, two purified, fucan-like sulfated polysaccharides having anticoagulant activity were obtained. We describe herein the isolation of the purified sulfated polysaccharides, their physical and chemical properties, and their blood-anticoagulant activities

Isolation, purification, and characterization of fucose-containing sulfated polysaccharides from the brown seaweed ecklonia kurome and their blood-anticoagulant activities

A sulfated polysaccharide fraction, obtained from the hot-water extract of the brown seaweed, Ecklonia kurome by removing laminaran and the major part of alginic acid, gave sulfated polysaccharides (B-I, B-II, C-I, and C-II) by both anion-exchange chromatography on a column of Ecteola-cellulose and by fractional precipitation with ethanol containing 0.3% calcium acetate, and then by gel-filtration chromatography on a Sepharose 4B column. B-I and B-II are composed of fucose, galactose, mannose, xylose, glucuronic acid, and ester sulfate in the approximate molar ratios of 1.00:0.36:0.48:1.08:1.85:2.35 and 1.00:0.81:0.18:0.45:0.61:2.00, respectively. C-I and C-II are composed of fucose, galactose, glucuronic acid, and ester sulfate in approximate molar ratios of 1.00:0.03:0.03:1.61 and 1.00:0.19:0.07:1.48, respectively. Blood-anticoagulant activities with respect to activated partial thromboplastin time (APTT) were approximately 24, 19, 81, and 85% of that of heparin for B-I, B-II, C-I, and C-II, respectively. All the polysaccharides showed slight antithrombin activity. No antifactor Xa activity was observed for any of the polysaccharides.

種々の褐藻由来フコース含有硫酸化多糖画分の糖組成と抗血液凝固作用

Fucose-containing sulfated polysaccharide (SPS) fractions were prepared from 85% methanol-treated materials of nine different species of brown seaweeds. They were extracted with diluted hydrochloric acid (pH 2) at room temperature and the extracts fractionated with aqueous 3% cetylpyridinum chloride and 2% CaCl2 solutions. Electrophoresis indicated that each of the obtained fractions consisted of more than two polymeric components. Chemical analysis and g. l. c. showed that each SPS fraction contained fucose, galactose, mannose, xylose, glucuronic acid and half ester sulfate as constituents. The proportions of the constituents varied widely from one fraction to another. Blood-anticoagulant activity tests were conducted on each SPS fraction with human or sheep plasma. The tests included thrombin time (TT), activated partial thromboplastin time (APTT), and anti-(factor Xa) activity in comparison with values of heparin (167 units/mg). When assayed for human plasma, all SPS fractions showed some TT(0-35 units/mg) and APTT (12-38 units/mg) activities, whereas anti-(factor Xa) activity was not remakable in any SPS fraction. Among the SPS fractions tested, that of Ecklonia kurome exhibited the highest activity with respect to TT (35 units/mg) and APTT (38 units/mg). When assayed for sheep plasma, the fraction of E. kurome showed higher activity than that determined for human plasma (activity with respect to TT and APTT was 120 and 95 units/mg).