Suitable Test Research Articles

Experimental study on anti-clamping damage structure of drill pipe against slip-crushing

Deep ground conditions bring higher challenges to drill pipe and slip tools, and the slip is more likely to seriously bite or crush the drill pipe, which will easily lead to failure of the drill pipe, seriously affecting the safety of drilling operations. Based on the theoretical analysis of the mechanical behavior and damage characteristics of the drill pipe held by slips, the deep ground suitability test of API-S135 conventional drill pipe and S135 and V150 anti-slip crushing drill pipe combined with micro-teeth marks hydraulic slips with thickened slip holding area is carried out. The test results show that: Compared with S135 common drill pipe, S135 and V150 anti-slip crushing drill pipe have an average change in outside diameter of 43% and 0.19%, with a reduction of 8.31% and 4.9% in the proportion of the maximum depth of the tooth mark, both of which have good mechanical resistance to slip crushing and biting, and the tensile load capacity of the clamped pipe body has been significantly improved. Combined with micro-tooth mark slips, the slips in deep drilling operations can tighten the drill pipe and make the bite damage of the drill pipe less. It can effectively ensure the safety and efficiency of deep drilling operations.

Analysis of Drinking Water Service in Shimla City, Himachal Pradesh

Water is an inevitable requirement for all. Existence of life without water cannot be imagined. Water scarcity is a burning issue and the need to tackle this problem is forefront issue. Shimla City, the capital of Himachal Pradesh is facing water scarcity consecutively from past many years. Though, administration is taking substantial steps to improve the situation, this problem is continuously witnessed by the residents every year. Being capital of the state, Shimla city is hub to various administrative units, educational and important health institutions also to name a few. Even the city is a famous tourist destination and internationally recognised one. All this drew the attention towards the growing need of water in the Shimla city. Since, residents are the first to face the problem of water scarcity, therefore, the present study tries to examine the situation of drinking water service at ground level using peoples’ perception. Hypotheses has been created with respect to certain parameters like relationship between hours of water supply and satisfaction, frequency of water supply with adequacy of quantity received using suitable testing methods. It has been found during the study that though the situation is improving but certain issues like frequency of water, awareness and responsible behaviour from residents is required.

Python's Role in Accelerating Web Application Development with Django

Efficient, secure, and scalable web services are critical in today's digital environment. This study explores the field of web service development and addresses issues such system efficiency, project duration, and changing requirements. The goal of this study is to ensure reliable and efficient web services by streamlining the development process and utilizing the Django framework. The study highlights the application of Django's Model-Template-View (MTV) design pattern, which is customized for a listing management system. Smooth data interaction and effective system performance are ensured by the study's seamless integration of Django's capabilities with MySQL for database management. To further improve system performance, focus is given to standardizing data sharing protocols and expediting user authentication procedures. The automation of web page construction with Python, HTML, and CSS modules, which increases system efficacy, is a crucial component of the research. Furthermore, the study shows how crucial security measures are, such as encryption for data confidentiality and integrity, which are made possible by the REST API interaction with the front end and the Django REST framework. Along with discussing key attacks and weaknesses that are frequent in web technology, the article also offers strategies to reduce these risks. Through the use of suitable SDLC models and testing procedures, it recognizes the crucial role that software engineering processes play in the development process. Focusing attention to Django's feature set, scalability, and wide library support, the study proves its advantages over competing web frameworks. The goal of the research is to provide developers and students with the skills they need to fully utilize Django's capabilities, making it easier to create dependable, successful and safe online applications. This study advances knowledge in the field of web technology and service development by lighting the development, difficulties, and current solutions in these fields. The study's ultimate goal is to open the door for comprehensive, modern, and efficient web service creation in order to satisfy the growing need for effective web solutions in the current digital era.

EFFECTS OF CLIMATE CHANGE ON ADAPTATION OF FARMERS IN NORTH WEST REGIONS OF HERAT PROVINCE

Agriculture and Climate are mutually dependent. There is a need to understand the effect of climate change on agricultural sector both at global and as well as at regional level. The present study was conducted in 2020, to know the effects of climate change on farmers’ adaptation. Ex post facto research design applied for this research. Pre prepared questionnaires have been distributed to120 respondents in three districts of north - west regions of Herat province and analyzed with the help of suitable statistical tests and the findings showed that, all farmers opined that, wheat’s varieties were changed from long duration to short duration, time of planting, switching over to other crops was observed among farmers. Spacing between the rows/plants, quantity of seeds used and quantity of organic fertilizer application were increased after 2010. The major constraint to initiate adaptation measures was; difficult to work in the field due to severe temperature and the important suggestion to mitigate the ill effects of climate change was; planning to control and store rainwater through the implementation of watershed management program. Based on the results of this study, it is hoped that appropriate strategies/ measures can be worked out to create awareness and learning mechanisms to enable farmers to handle their situations better.

Performance evaluation of the Panbio COVID-19/Flu A&B Panel for detection of SARS-CoV-2, influenza A, and influenza B antigens using mid-turbinate nasal swabs.

The Panbio COVID-19/Flu A&B Panel is a suitable rapid test for detecting COVID-19 and influenza in symptomatic subjects across diverse global populations, exhibiting high sensitivity. The assay achieved a sensitivity of 94.0% in samples with Ct ≤24 for COVID-19 and 92.6% in samples with Ct ≤30 for influenza A and B.

Blow-up of solutions to the wave equations with memory terms in Schwarzschild spacetime

The main goal of this work is to discuss formation of singularities of solutions to the Cauchy problems for single wave equation and coupled system of wave equations with nonlinear memory terms in Schwarzschild spacetime. For the initial value problem of nonlinear wave equations, blow-up results of sign changing solutions are derived by employing the suitable test function technique which is related to the Riemann-Liouville fractional derivative and contradiction argument. Additionally, upper bound lifespan estimates of solutions to the small initial value problem of wave equations are established by imposing certain conditions on the initial values. It is worth to mention that crucial difficulty is the setting of test function framework, which has not been applied to the Schwarzschild spacetime in the previous literatures. To the best of our knowledge, the results in Theorems 1.1-1.4 are new.

Marine digital twins for enhanced ocean understanding

Understanding the oceans requires accurate monitoring and suitable test environments for conducting hypothesis-driven simulations. Marine digital twins can be part of the solution to foster knowledge on the way to a sustainable blue economy. Herein we adapt the current literature on digital twins (DTs) in the industrial domain to the marine domain and reinterpret the dimensions of DTs to build a marine digital twin (MDT). These dimensions are the physical entity, the data, the services, and the virtual models. The latter represents the core aspect of DTs that bridges between the other dimensions. It consists of graphical models, physical models and especially methodologies of artificial intelligence as well as machine learning algorithms. The presented dimensions provide a conceptual view to identify missing components when building MDTs and provide researchers and developers guidance for developing MDTs. To exemplify these dimensions, we present two of our projects where MDTs are developed: NorthSat-X and AI4DTE. In NorthSat-X essential climate variables of the North Sea are incorporated into a MDT. Moreover, in AI4DTE a digital prototype is developed to monitor anomalies of offshore wind farms. At the end, we identified that MDTs face three main challenges that are subject of the paper’s discussion: scalability, interoperability, and explainability.

Concordance between tissue (tumor DNA) and liquid (ctDNA) biopsy next-generation sequencing (NGS) data in detection of PIK3CA, AKT1, and PTEN alterations in breast cancer: A retrospective analysis.

e15033 Background: In the Phase 3 CAPItello-291 trial (NCT04305496), the addition of capivasertib (a potent, selective pan-AKT inhibitor) to fulvestrant significantly improved progression-free survival in patients with HR+/HER2− advanced breast cancer, especially in those with PIK3CA/AKT1/PTEN-altered tumors identified using tissue biopsy-based FoundationOneCDx. Given the growing utility of circulating tumor DNA (ctDNA) and interest in liquid biopsy-based tests to detect PIK3CA/AKT1/PTEN alterations, we performed a retrospective analytical comparison of FoundationOneCDx and blood-based FoundationOneLiquidCDx NGS data. Methods: We utilized the FoundationCORE database of breast cancer cases profiled during routine clinical care with FoundationOneCDx and/or FoundationOneLiquidCDx (covering the same genomic regions). Analytical comparison of all pathogenic PIK3CA/AKT1/PTEN alterations as well as alterations defined in the CAPItello-291 protocol (CAPItello-291 alterations) was performed in paired data from cases with both tissue and liquid biopsies, sampled within 90 days of each other. Subgroup analysis was conducted based on ctDNA tumor fraction (TF). Data from liquid biopsies were analyzed for co-occurring mutations. Results: CAPItello-291 alterations were detected in 44.9% of 35,730 tissue biopsies and 34.3% of 7056 liquid biopsies profiled from Jan 2017–Jun 2023. In paired NGS data (n=289), the overall positive percent agreement (PPA) for CAPItello-291 alterations across ctDNA TF subgroups was: TF ≥10% (n=99): 92.5%; TF 1–10% (n=66): 97.1%; TF <1% (n=124): 33.9%. The PPA for all PIK3CA/AKT1/PTEN short variants (base substitutions, indels) analyzed was: ctDNA TF ≥10%: PIK3CA, 93.9%; AKT1, 100%; PTEN, 100%, and ctDNA TF 1–10%: PIK3CA, 96.3%; AKT1, 100%; PTEN, 100%. For PTEN biallelic deletions, the PPA was 50% (2/4) in cases with ctDNA TF ≥10%. In liquid biopsies with CAPItello-291 alterations (n=2421), the most frequent co-occurring mutations were in TP53 (49.9%), ESR1 (31.2%), CDH1 (23.7%), NF1 (13.0%), RB1 (12.8%), CHEK2 (12.4%), ATM (11.9%), FGF3 (11.4%), FGF19 (11.0%), and FGF4 (10.5%). Mutations in BRCA1 (2.4%), BRCA2 (4.8%), and PALB2 (1.3%) were also observed. Conclusions: PPA between liquid and tissue biopsy NGS data for the detection of PIK3CA/AKT1/PTEN short variants was high in cases with ctDNA TF ≥1%. Liquid biopsies offer a minimally invasive approach for the detection of some CAPItello-291 alterations. However, limitations posed by ctDNA TF <1% and sample and variant types should be considered. Together, these data can help clinicians make informed decisions regarding suitable diagnostic tests to determine patient eligibility for breast cancer therapies.

Evaluation of SiL Testing Potential—Shifting from HiL by Identifying Compatible Requirements with vECUs

Due to the increasing complexity of vehicle software, it is becoming increasingly difficult to comprehensively test all requirements. This inevitably means that alternative test methods, e.g., simulation-based methods, must be used more frequently. However, the challenge involves identifying appropriate requirements that can be technically tested in a simulation environment initially. The present work is aimed at evaluation and optimization of the effectiveness of software-in-the-loop (SiL) simulations in the testing process of vehicle software. The focus is on supporting the testing process by shifting specific test cases from hardware-in-the-loop (HiL) test benches to SiL-based simulations. For this purpose, a systematic approach was developed to analyze and categorize requirements, enabling precise and efficient allocation of test cases. Furthermore, a detailed review and recommendation for improving the ProSTEP iViP standard for virtual electronic control units (vECU) was carried out. The developed matrix associates the defined requirement clusters with different classifications of vECUs, facilitating the identification of suitable test environment types for conducting specific test cases. By assigning test cases to appropriate vECU levels, the testing processes can be targeted and cost-optimized. Finally, the theoretical results were evaluated in an SiL simulation environment. It was observed that a significant part of the requirements could effectively be tested using a vECU. These findings confirmed the potential of SiL simulation environments to not only support, but also enhance, the testing process for vehicle software by providing a cost-effective and flexible complement to traditional HiL test benches.

Practical Evaluation of Ionic Liquids for Application as Lubricants in Cleanrooms and under Vacuum Conditions

As part of a publicly funded cooperation project, novel high-performance lubricants (oils, greases, assembly pastes) based on ionic liquids and with the addition of specific micro- or nanoparticles are to be developed, which are adapted in their formulation for use in applications where their negligible vapor pressure plays an important role. These lubricants are urgently needed for applications in cleanrooms and high vacuum (e.g., pharmaceuticals, aerospace, chip manufacturing), especially when the frequently used perfluoropolyethers (PFPE) are no longer available due to a potential restriction of per- and polyfluoroalkyl substances (PFAS) due to European chemical legislation. Until now, there has been a lack of suitable laboratory testing technology to develop such innovative lubricants for extreme niche applications economically. There is a large gap in the tribological test chain between model testing, for example in the so-called spiral orbit tribometer (SOT) or ball-on-disk test in a high-frequency, linear-oscillation test machine (SRV-Tribometer from German “Schwing-Reib-Verschleiß-Tribometer”), and overall component testing at major space agencies (ESA—European Space Agency, NASA—National Aeronautics and Space Administration) or their service providers like the European Space Tribology Laboratory (ESTL) in Manchester. A further aim of the project was therefore to develop an application-orientated and economical testing methodology and testing technology for the scientifically precise evaluation and verifiability of the effect of ionic liquids on tribological systems in cleanrooms and under high vacuum conditions. The newly developed test rig is the focus of this publication. It forms the basis for all further investigations.

Black-Box Testing and Auditing of Bias in ADM Systems

For years, the number of opaque algorithmic decision-making systems (ADM systems) with a large impact on society has been increasing: e.g., systems that compute decisions about future recidivism of criminals, credit worthiness, or the many small decision computing systems within social networks that create rankings, provide recommendations, or filter content. Concerns that such a system makes biased decisions can be difficult to investigate: be it by people affected, NGOs, stakeholders, governmental testing and auditing authorities, or other external parties. Scientific testing and auditing literature rarely focuses on the specific needs for such investigations and suffers from ambiguous terminologies. With this paper, we aim to support this investigation process by collecting, explaining, and categorizing methods of testing for bias, which are applicable to black-box systems, given that inputs and respective outputs can be observed. For this purpose, we provide a taxonomy that can be used to select suitable test methods adapted to the respective situation. This taxonomy takes multiple aspects into account, for example the effort to implement a given test method, its technical requirement (such as the need of ground truth) and social constraints of the investigation, e.g., the protection of business secrets. Furthermore, we analyze which test method can be used in the context of which black box audit concept. It turns out that various factors, such as the type of black box audit or the lack of an oracle, may limit the selection of applicable tests. With the help of this paper, people or organizations who want to test an ADM system for bias can identify which test methods and auditing concepts are applicable and what implications they entail.

The Effects of Language Background and Parental Education on Measures of Cognitive Ability: An Analysis of the WPPSI-IV Cognitive Profiles of Monolingual, Simultaneous Bilingual, and Sequential Bilingual German Children Aged 4 to 7 Years.

The present study investigated the possible effects of language background (monolinguals, simultaneous bilinguals, and sequential bilinguals) and parental education (no/low, medium, high, and highest parental education) on measures of cognitive ability provided by the Wechsler Primary and Preschool Scale-Fourth Edition (WPPSI-IV). Statistical analyses were based on a sample of 290 children (130 females, 160 males). Three multivariate variance analyses were conducted to identify possible effects. In cases of statistically significant main effects, post hoc analyses were additionally performed to identify group differences. The results indicated that simultaneous bilinguals performed more similarly to monolinguals than sequential bilinguals. On average, sequential bilinguals achieved significantly lower scores on the Verbal Comprehension Index (VCI), the Vocabulary Acquisition Index (VAI), and the associated subtests than monolinguals and simultaneous bilinguals. Significantly lower average scores on VAI and the associated subtests were found for simultaneous bilinguals compared to monolinguals. Children with parents having no, a lower, or a medium educational level achieved significantly lower scores on VCI, VAI, and the FSIQ than children with parents having a high or highest educational level on average. The present findings suggest that the WPPSI-IV represents a suitable and reliable test battery for the assessment of cognitive skills in children with different language backgrounds and parental educational levels.

Predictive language processing: integrating comprehension and production, and what atypical populations can tell us.

Predictive processing, a crucial aspect of human cognition, is also relevant for language comprehension. In everyday situations, we exploit various sources of information to anticipate and therefore facilitate processing of upcoming linguistic input. In the literature, there are a variety of models that aim at accounting for such ability. One group of models propose a strict relationship between prediction and language production mechanisms. In this review, we first introduce very briefly the concept of predictive processing during language comprehension. Secondly, we focus on models that attribute a prominent role to language production and sensorimotor processing in language prediction ("prediction-by-production" models). Contextually, we provide a summary of studies that investigated the role of speech production and auditory perception on language comprehension/prediction tasks in healthy, typical participants. Then, we provide an overview of the limited existing literature on specific atypical/clinical populations that may represent suitable testing ground for such models-i.e., populations with impaired speech production and auditory perception mechanisms. Ultimately, we suggest a more widely and in-depth testing of prediction-by-production accounts, and the involvement of atypical populations both for model testing and as targets for possible novel speech/language treatment approaches.

Which Scale to Assess Pain Self-efficacy Shows Better Measurement Properties in Chronic Low Back Pain? A Head-To-Head Comparison Study

ObjectiveTo compare the quality of the measurement properties of Pain Self-Efficacy Questionnaire (PSEQ)-10, PSEQ-4, PSEQ-2, Chronic Pain Self-Efficacy Scale (CPSS) long-form, and CPSS short-form (CPSS-SF) in patients with chronic low back pain (CLBP). DesignCross-sectional and longitudinal studies (measurement properties). SettingOutpatient rehabilitation. ParticipantsParticipants (N=245) with nonspecific CLBP (18-60y, 63% women) were enrolled in this study. InterventionsNot applicable. Main Outcome MeasuresPain self-efficacy questionnaires were administered on 3 occasions: baseline assessment, 1 week after the first assessment (reliability), and after an 8-week exercise program (responsiveness). The intraclass correlation coefficient (ICC) and Cronbach α were used to assess reliability and internal consistency, respectively. Pearson correlation and confirmatory factor analyses were used to assess construct validity. The area under the curve and hypothesis testing were used to assess responsiveness. ResultsNo difference was observed for all the questionnaires regarding internal consistency (Cronbach α>.7), criterion validity (r>.88), and reliability (ICC>.7). The scales confirmed >75% of the hypotheses for the construct validity, except for CPSS-SF. PSEQ-2 did not meet the criterion for structural validity. PSEQ-10 met all the criteria for good measurement properties according to Consensus-Based Standards for the Selection of Health Measurement Instruments. ConclusionsIt was not possible to calculate structural validity for PSEQ-2, CPSS-SF did not meet the criterion for suitable hypothesis testing for construct validity, and all the questionnaires did not show suitable measurement error, except for the PSEQ-10. Hence, the PSEQ-10 was the unique scale that met all the criteria for good measurement properties for assessing pain self-efficacy in CLBP.

An alternative for the robust assessment of the repeatability and reproducibility of analytical measurements using bivariate dispersion

IntroductionAssessing repeatability and reproducibility in analytical chemistry is commonly based on parametric dispersion indicators, such as relative standard deviation and standard deviation, calculated for each detected variable using repeated measurements of Quality Control (QC) samples collected throughout the data acquisition sequence. However, their reliability strongly relies on the assumption of normality distribution. Knowing that analytical variability is conditional to many sources, the use of such parametric estimators is not always suitable. There is therefore a need for robust indicators of data quality independent of central values and any parametric assumption. MethodsThree specific indicators were developed: (i) intra-group dispersion, based on the median area of the convex hull of QC samples within an analytical batch; (ii) inter-group dispersion, defined as the gradient of the deviation between analytical batches; and (iii) dispersion index. Mathematical properties of these indicators, including positivity, stability, and translation invariance, were then evaluated using synthetic data under normal and non-normal distributions. Finally, the relevance of these indicators and the associated visualization methods were highlighted based on a metabolomics case study involving liquid chromatography coupled to mass spectrometry measurements of the NIST SRM1950 reference material analyzed over more than one year within different projects. ResultsThe proposed indicators were shown to be translation invariant and always positive, while first investigations performed on synthetic data revealed a high stability for multiplication. Moreover, their application to experimental data revealed specific behaviors depending on the characteristics of the signal associated with the different detected analytes, showing their ability to capture the variability observed either in parametric or non-parametric conditions. Moreover, this investigation showed different structures of sensitivity to analytical variability all along the data processing steps. The proposed indicators also allowed a visualization of the analytical drift in two dimensions, to facilitate result interpretation. ConclusionThese indicators open the way to a better and more robust assessment of repeatability and reproducibility but also to improvements of long-term data comparability involving suitability testing.

Pengembangan Media Video Tutorial Sulaman Pita Pada Elemen Desain Hiasan Di SMKS Dharma Wanita Gresik

This research aims: (1) To determine the feasibility of video tutorial media for ribbon embroidery on decorative design elements for class XI students at SMK Dharma Wanita Gresik (2) To determine the response of class Gresik Women. The research was carried out at Surabaya State University. The type of research used is quantitative descriptive. This research method uses R&D or Research and Development. This research instrument uses material suitability tests, media suitability and student responses. The results of this research state that: (1) the level of feasibility of developing ribbon embroidery tutorial video media by media experts and material experts is an average of 3.35 with a feasible category (2) student responses to developing ribbon embroidery video tutorial media The total percentage score obtained is above 94% is categorized as very good.

A Lagrangian approach for solving an axisymmetric thermo-electromagnetic problem. Application to time-varying geometry processes

The aim of this work is to introduce a thermo-electromagnetic model for calculating the temperature and the power dissipated in cylindrical pieces whose geometry varies with time and undergoes large deformations; the motion will be a known data. The work will be a first step towards building a complete thermo-electromagnetic-mechanical model suitable for simulating electrically assisted forming processes, which is the main motivation of the work. The electromagnetic model will be obtained from the time-harmonic eddy current problem with an in-plane current; the source will be given in terms of currents or voltages defined at some parts of the boundary. Finite element methods based on a Lagrangian weak formulation will be used for the numerical solution. This approach will avoid the need to compute and remesh the thermo-electromagnetic domain along the time. The numerical tools will be implemented in FEniCS and validated by using a suitable test also solved in Eulerian coordinates.

Evaluating proficiency testing implementation and identifying challenges that government comprehensive specialized hospital laboratories in northwest Ethiopia faced as they participated in the external quality assessment scheme: A document-based, interview-driven evaluation.

To assess the implementation of proficiency testing in the northwest Ethiopian government comprehensive specialized hospital laboratories, with a focus on identifying and understanding the challenges encountered during their participation in the external quality assessment scheme. A cross-sectional study was conducted among 3 comprehensive specialized hospitals in northwest Ethiopia, analyzing 41 documented laboratory test parameters from 2020 to 2022. In addition, face-to-face, in-depth interviews were carried out to identify the major challenges the participating institutions faced. The study covered a total of 41 tests across 9 cycles. Overall, proper implementation of proficiency testing was observed in 59.3% of the tests, with 61.8% maintaining consistent implementation status over 3 consecutive years. In addition, the overall performance of the laboratory was 54.3%, with a 68.7% participation rate. The predominantly identified challenges included the lack of participation, insufficient reagents and supplies, inadequacy of suitable proficiency testing materials, equipment malfunction and downtime, lack of management support, insufficient budget, and inadequate training and awareness. The results of this study highlight the ineffective implementation of proficiency testing. Contributing factors include personnel issues, equipment and supplies challenges, managerial shortcomings, difficulties with proficiency testing providers, budgetary constraints, and a lack of training and motivation.

Copeptin Stimulation by Combined Intravenous Arginine and Oral LevoDopa/Carbidopa in Healthy Short Children and Children with the Polyuria-Polydipsia Syndrome

Introduction: Stimulated copeptin may provide an alternative to water deprivation testing (WDT) in the evaluation of polyuria-polydipsia syndrome (PPS). Though best studied, arginine stimulation alone produces a modest copeptin response in children. We investigated the effectiveness of the arginine + LevoDopa/Carbidopa stimulation test (ALD-ST) for copeptin. Methods: 47 healthy short children (controls), 10 children with primary polydipsia, and 10 children with AVP deficiency received arginine hydrochloride (500 mg/kg intravenously over 30 min) and Levodopa/carbidopa (10:1 ratio; 175 mg of l-Dopa/m2 BSA) orally. Serum copeptin was measured at 0, 60, 90, and 120 min. Results: In controls, ALD-ST increased copeptin from a median of 7.0 pmol/L (IQR 5.0–10.0) to a peak of 44.0 pmol/L (IQR 21.4–181.0) between 60 and 120 min (p < 0.001). Copeptin peak was higher in subjects who experienced nausea or vomiting (57%) than in those who did not (131.0 pmol/L [IQR 42.5–193.8] vs. 22.7 pmol/L [IQR 16.0–33.7], p < 0.001). While subjects with primary polydipsia had similar baseline (8.5 pmol/L [IQR 8.0–11.0]) and stimulated (125.2 pmol/L [IQR 87.6–174.0]) copeptin levels as controls, subjects with AVP deficiency had lower baseline (2.5 pmol/L [IQR 2.0–3.1]) and peak levels (4.6 pmol/L [IQR 2.4–6.0]). A peak copeptin of ≥9.3 pmol/L best predicted absence of complete or partial AVP deficiency with a sensitivity of 100% and specificity of 80%. Conclusions: ALD-ST induced a robust peak copeptin in healthy short children and children with primary polydipsia. Nausea/vomiting, a side effect of ALD-ST, amplified the copeptin response. The ALD-ST may be a suitable initial screening test in children with PPS.

Two chromatographic methods for determination of cytarabine and dexamethazone; two co-administered drugs for the treatment of leukemia: green analytical perspective

Two sensitive, straightforward and repeatable chromatographic techniques were developed for the determination of Cytarabine HCl and Dexamethazone in their pure form and spiked human plasma without prior separation. The drugs are used co-administered for the treatment of Leukemia, a certain type of blood cancer. Method (A) is an isocratic chromatographic HPLC method; separation was accomplished on C18 column using the eluting mixture of 6.9 g/L Monobasic Sodium Phosphate pH 3: methanol (70:30, v/v) and detection was at 275 nm. Concentrations were in the range of 0.2–15 μg/mL for both CYT and DEX. Method (B) is a HPTLC method in which separation was attained on HPTLC F254 plates using methanol: ethyl acetate: ammonia, (7.8:2:0.2, by volume) as eluting solvents and detection was at 275 nm. Concentrations were in the range of 0.1–4 μg/band for both CYT and DEX. The parameters for system suitability testing were evaluated to determine the effectiveness of the developed chromatographic procedures in terms of performance. The recently developed techniques were applied for the determination of the drugs under investigation in spiked human plasma. Validation parameters were examined in accordance with US-FDA criteria. All results were found to be within the acceptable ranges. To evaluate the greenness characters of the proposed methods to the environment; three greenness assessment tools including eco-scale assessments (ESA), green analytical procedure index (GAPI), and Analytical Greenness calculator (AGREE) were used. Acceptable and satisfying results that demonstrated the greenness characteristics of the suggested methods were attained.