Suitable Test Research Articles

Turbulence and flapping pivot axis effects on torsional flutter harvester efficiency by closed-form formula

This “Short Communication” investigates the dynamics of a torsional-flutter energy harvester in atmospheric winds with stationary turbulence. This apparatus is an example of a flutter mill, which operates by exploiting aeroelastic instability as a competitive alternative and as a renewable energy supply for one or few housing units. The apparatus has a rigid blade-airfoil that rotates about a pivot to generate flapping motion. Contrary to recent studies by the second author, the effect of random stationary turbulence on flutter onset is examined by an analytical approach, employed by Scanlan (1997) for bridge flutter analysis. Turbulence effect is simulated by suitably modifying the span-wise coherence equation of the aeroelastic load. The incipient flutter threshold is found as a function of turbulence properties. Various configurations are studied, i.e., pivot position, aspect ratio, turbulence coherence decay parameter and structural damping. The objective is to perform a thorough sensitivity analysis as the necessary premise for the planned, future examination of post-critical instability and operational efficiency of the harvester by suitable modeling and wind tunnel tests.

Producing plankton classifiers that are robust to dataset shift

AbstractModern plankton high‐throughput monitoring relies on deep learning classifiers for species recognition in water ecosystems. Despite satisfactory nominal performances, a significant challenge arises from dataset shift, which causes performances to drop during deployment. In our study, we integrate the ZooLake dataset, which consists of dark‐field images of lake plankton (Kyathanahally et al. 2021a), with manually annotated images from 10 independent days of deployment, serving as test cells to benchmark out‐of‐dataset (OOD) performances. Our analysis reveals instances where classifiers, initially performing well in in‐dataset conditions, encounter notable failures in practical scenarios. For example, a MobileNet with a 92% nominal test accuracy shows a 77% OOD accuracy. We systematically investigate conditions leading to OOD performance drops and propose a preemptive assessment method to identify potential pitfalls when classifying new data, and pinpoint features in OOD images that adversely impact classification. We present a three‐step pipeline: (i) identifying OOD degradation compared to nominal test performance, (ii) conducting a diagnostic analysis of degradation causes, and (iii) providing solutions. We find that ensembles of BEiT vision transformers, with targeted augmentations addressing OOD robustness, geometric ensembling, and rotation‐based test‐time augmentation, constitute the most robust model, which we call BEsT. It achieves an 83% OOD accuracy, with errors concentrated on container classes. Moreover, it exhibits lower sensitivity to dataset shift, and reproduces well the plankton abundances. Our proposed pipeline is applicable to generic plankton classifiers, contingent on the availability of suitable test cells. By identifying critical shortcomings and offering practical procedures to fortify models against dataset shift, our study contributes to the development of more reliable plankton classification technologies.

Sediment TIE Validation Study Using South Korean Domestic Species Glyptotendipes tokunagai to Evaluate Contamination with Two Heavy Metals and Ammonia

Toxicity identification evaluation (TIE) is used to support sustainable soil management. However, TIE studies for sediment contamination have been limited and no previous study has validated its applicability to a species native to South Korea, to the best of our knowledge. Therefore, this study aims to validate the applicability of domestic species inhabiting East Asia to a TIE framework. We selected Glyptotendipes tokunagai, a common benthic invertebrate in urban areas. Contaminated sediment samples were generated using cadmium, copper, and ammonia, which are the major pollutants in sediment. Three different adsorbents were chosen as pollutant removal agents to validate the applicability of TIE using G. tokunagai. In the results, G. tokunagai exhibited a mortality rate that was dependent on the concentration of each pollutant. The mortality rate dramatically decreased after adding the chosen adsorbents to the contaminated sediments. Additionally, the observed reduction in toxicant concentrations suggests that these additives are efficient for toxicant removal. In contrast, WCA10L showed a mortality higher than 20% and seems not to be a proper additive for a toxicity assay. Through this study, G. tokunagai was demonstrated to be sufficiently sensitive to sediment contaminated with different pollutants and showed reproducible measurements, making it a suitable test organism for the TIE framework. Moreover, suitable adsorbents were suggested by measuring efficiency and toxicity after the toxicant removal procedure.

Neurodynamic testing of the suprascapular nerve: An observational cadaveric study

BackgroundSuprascapular neurodynamic tests are used to clinically test for suprascapular nerve mechanosensitivity. Various tests described in the literature are proposed to induce suprascapular nerve mechanical strain but their potential effects on nerve strain have not been established. MethodsThis observational cadaveric study used biomechanical measurements to evaluate and compare the strain undergone by the suprascapular nerve during five different neurodynamic positions: (1) Cervical contralateral rotation and scapular retraction; (2) Cervical contralateral sidebending + shoulder girdle depression; (3) Shoulder girdle depression, retraction, posterior tilt and downward rotation; (4) Position 3 + contralateral cervical sidebending; and (5) Scapular protraction + Cervical contralateral sidebending (protraction sidebending test). Suprascapular nerve strains were measured using two differential transducers, one placed before the nerve entry by the suprascapular notch (prescapular portion) and the second after the spine of the scapula in the infraspinal fossa (scapular portion). FindingsPrescapular portion: all 5 positions induced a mean strain increase of 22.70 % (95 %CI 17.14, 28.26) with no significant differences between positions (p > 0.05). Scapular portion: the protraction sidebending test provoked the highest strain increase of 8.99 % (95 %CI 6.83, 11.15), significantly greater than the other positions (p < 0.05). InterpretationAll 5 positions increased suprascapular nerve strain of the prescapular portion of the suprascapular nerve. The protraction sidebending test induced the highest strain of the scapular portion of the nerve. As it is likely to influence both portions of the suprascapular nerve, the protraction sidebending test is the most suitable neurodynamic test to test for suprascapular heightened mechanosensitivity.

The Evaluation of Compost Maturity and Ammonium Toxicity Using Different Plant Species in a Germination Test

The determination of the maturity of compost and other organic fertilizers is very important because of possible phytotoxic or phytostimulating effects. The maturity of compost can be assessed on the basis of chemical analyses, and a germination test with different test plants is most often used to determine phytotoxicity. In this research, the maturity of compost produced from the plant residues subsequent to the maintenance of green public areas was assessed using the results of chemical analyses. Simultaneously, a germination test was carried out with the four test plant species (cucumber, garden cress, triticale, and barley) to determine the phytotoxicity of compost extract in a ratio of 1:2.5 v/v (1:3.3 w/v) and 1:10 v/v (1:13.3 w/v) and the three ammonium N solutions (in the concentrations of 200, 400, and 600 mg/L NH4-N). According to the chemical properties of compost (primarily the C/N, NH4-N/NO3-N ratios, and the NH4-N concentration) and the germination test with cucumber and garden cress, we may conclude that the tested compost was mature and that we did not expect a phytotoxic effect. The choice of a plant is very significant because the germination test with a compost extract demonstrated an undoubted phytostimulating effect on the garden cress and cucumber, with a more pronounced phytostimulating effect of the 1:10 than that of the 1:2.5 v/v compost extract. No such effect was detected on the monocotyledonous test plants triticale and barley since the 1:10 v/v extract had no significant effect, and the 1:2.5 v/v extract had a phytotoxic effect, moderate on the triticale and high on barley. The conclusion is that garden cress and cucumber are suitable test plants for the determination of compost’s phytostimulative effect, but they are not suitable for the determination of phytotoxicity for monocotyledonous plants, especially if the cause of phytotoxicity is a non-ammonium component. Barley is the most suitable species for the determination of compost’s non-ammonium phytotoxicity and nitrogen’s ammonium-form phytostimulative or phytotoxic effect. It would be very useful to conduct a comparative germination test with the compost extracts in the ratios 1:2.5 and 1:10, whereby the 1:2.5 extract would be used as a test of phytotoxicity, and the 1:10 extract for the test of a phytostimulating effect.

Acceptability and utility of a broad test battery to evaluate physical functioning and frailty in community-based settings for people experiencing homelessness and addiction–Findings from the LEAP trials

BackgroundPhysical function deficits and frailty are commonly reported in people experiencing homelessness and addiction. Typically, geriatric, or general physical performance measures are employed to evaluate these constructs. Objectives and settingWe aimed to investigate the feasibility of a broad test battery to evaluate physical functioning and frailty in a non-geriatric community-based population of people experiencing homelessness and addiction. Design and methodsPhysical function and the presence of frailty was evaluated using combined data from two linked low-threshold exercise pre-post intervention studies (LEAP I and II). ParticipantsSeventy-four participants attending an open-access day-service centre (18 to 65 years) were evaluated. Participants with acute confusion or behavioural issues, or major medical condition precluding safe participation were excluded. InterventionA broad test battery was performed which evaluated physical functioning and frailty. Main outcome measuresOutcome measures included were the Short Physical Performance Battery, Single Leg Stance, Chair Stand Test, 10 m and 2 minute Walk Tests, limb circumference, dynamometry, Clinical Frailty Scale, SHARE-Frailty Instrument, numerical pain rating and Short Form-12. ResultsTest completion was 100% for all outcomes except pain rating (81% and 79% for LEAPI and II, respectively), and balance and walking tests (95% and 93% in LEAP II). A ceiling effect was detected for the Short Physical Performance Battery. ConclusionHigh feasibility of the test batteries was demonstrated. An optimised test battery was proposed based on findings. This has important implications for clinicians working in inclusion health settings. Clinical Trial Registration NumberClinical Trials.gov: NCT05700305. Contribution of the Paper•This paper proposes a suitable test battery to enable clinicians to evaluate physical functioning and frailty in people experiencing homelessness and addiction challenges in community settings.•There is a paucity of research evaluating outcome measures for this population and no core outcome set has been reported.•This test battery would allow the effect of treatment and targeted interventions to be evaluated in this marginalised population.

Portable near-infrared detection to replace color tests in an analytical scheme for forensic drug identification

In the ever-changing drug market the popularity and availability of substances is dynamic. In the Netherlands, ketamine and various cathinones have recently seen increased prevalence. It is crucial for law enforcement to quickly obtain an initial indication of the identity of a substance. This first test also serves as quality control for subsequent confirmation with GC–MS analysis. Traditionally, color tests have been used for these purposes. While these tests are quick and inexpensive, they have the disadvantage of reacting only to a few traditional drugs. Suitable color tests are not available for many new psychoactive substances (NPS). Near-infrared (NIR) spectroscopy is a rapid technique that provides a characteristic spectrum for organic compounds. This technique is more versatile than color tests and can adapt more quickly to market changes by incorporating reference spectra into the library. This study demonstrates the feasibility of obtaining a good quality NIR spectrum from a 20 mg sub-sample in a test tube. This was achieved by scanning the test tube through the glass bottom. In the routine analytical scheme, these test tubes were subsequently batchwise analyzed by GC–MS. From the NIR spectra, 84 % true positive and 100 % true negative results were achieved on 516 casework samples, including identification of substances without available color tests like ketamine. Missed false negatives primarily involved new substances absent from the library, emphasizing the need for continuous library updates. NIR’s adaptability to market changes is crucial, allowing the inclusion of new substances as they emerge. This method enhances law enforcement’s ability to make informed decisions, aiding in the indictment process.

Evaluation of Clozapine-induced Toxicities in Psychic Patients in Mental Health Care Hospitals in the Southern Region of the Saudi Arabia Kingdom

Background: Clozapine is a psychiatric medication that may cause various side effects, some of them may be serious and fatal adverse effects, such as severe neutropenia, agranulocytosis, lymphocytopenia, myocarditis, and orthostatic hypotension, that have been associated to an increased risk of death. Objective: This study aimed to evaluate the serious and potentially fatal adverse effects of clozapine toxicity in psychic patients at mental health care hospitals in the Southern region of the Kingdom of Saudi Arabia. Methods: By using a survey, data were retrospectively collected from 193 adult psychic patient reports who have been administrated clozapine with regular follow-ups, in mental health hospitals in the Southern region of Saudi Arabia between 2019 and 2021. Then, these data are recorded and analyzed Statistically using SPSS software, with suitable tests, and predetermined statistical significance (p-value) of less than 0.001. Results: The occurrence of agranulocytosis, neutropenia, hypotension, and seizures showed a highly significant correlation with higher doses of clozapine administration (i.e. p &lt; 0.001). Similarly, agranulocytosis and neutropenia were significantly associated with the occurrence of both hypotension and seizures (i.e. p &lt; 0.001). Conclusion: The collected data in this study showed an increased incidence of agranulocytosis and neutropenia associated with clozapine-treated psychic patients in the Southern region of the Kingdom of Saudi Arabia which warrants further clinical studies to find this correlation.

A stress test method of polycrystal 95%Al2O3 based on Vickers indentation-induced cracks

IntroductionPolycrystalline alumina is a critical material in the containment of nuclear reactors, and its stress must be evaluated. ObjectiveThe current Vickers fracture residual stress test methods are mainly used for amorphous materials. To realize the preliminary application in engineering practice, exploring suitable stress test methods for polycrystalline alumina and testing the micro-zone stress of 95%Al2O3 in 4J33/95%Al2O3brazed joints is necessary. MethodsThe specimens were loaded with different stress states by pre-stressing tooling, and the stress distribution in the test area was clarified through finite element analysis. Then, Vickers indentation experiments were carried out to count the indentation radius a, the crack length l, and the direction of crack propagation θ. ResultsWith the crack size not exceeding the grain size and the effects of microstructure (intergranular pores, intragranular pores, and grain boundaries) and crystal orientation on the crack projection length reduced, a model was developed for calculating the relationship between the crack projection length and the pre-stress of polycrystalline alumina at low load indentation in Palmqvist, with a maximum absolute deviation of 23.3 MPa and a maximum relative error of 49.7 %. The microzone stresses on the ceramic side of 4J33/95 % Al2O3brazed joints were characterized. ConclusionThe stress detection method based on Vickers indentation cracks proposed in this study provides a new idea for evaluating residual stresses in polycrystalline alumina; however, the universality of this method in polycrystalline alumina with different grain sizes and its applicability in other polycrystalline brittle materials can still be further explored.

Translating mineral systems criteria into a prospectivity model for IOCG deposits in the Kolari region, Finland

The mineral system approach facilitates mapping the fingerprints of geological processes in ore formation using mineral prospectivity modeling (MPM). It aims to reduce the time and cost of exploration as a priority in the mineral exploration industry. Finland, which is covered with thick soil, dense vegetation, and snow in winter and thus has limited outcrops, serves as a suitable testing ground for this method. In this contribution, MPM was employed to predict favorable targets for Fe-Cu-Au (IOCG) deposits in the Kolari region, northwestern Finland. The mineral system components ore metal and sulfur sources, pathways, energy sources/drivers, traps, and the geological factors influencing the ore-forming processes were translated into mappable criteria and were considered for a regional-scale analysis. Knowledge-driven, fuzzy logic overlay, fuzzy inference system, and geometric average methods integrate evidential layers derived from geological (scale-free geology map), geochemical (till and bedrock sample geochemistry), and geophysical data (magnetic, radiometric, electromagnetic measurements, and gravity worms). Subsequently, the data were modeled using a combined conceptual/empirical approach, i.e. fuzzified evidential layers and logistic regression. In the prospectivity models, existing IOCG deposits are consistently placed within high-favorability areas. In addition, new exploration targets were identified. The models were validated against known IOCG deposits using Receiver Operating Characteristic (ROC) analysis and average Area Under the Curve (AUC) with values of final prospectivity models consistently reaching scores > 0.8. This indicates a favorable outcome of the application of MPM, making the approach applicable to other regions and ore deposit types. A majority voting ensemble technique was employed to combine the favorable areas of each prospectivity model. Then a confidence index was adopted reducing uncertainty linked to the models generated. These outputs consistently facilitated the identification of exploration targets more reliably.

The Photometric Testing of High-Resolution Digital Cameras from Smartphones-A Pilot Study.

Luminance is the fundamental photometric quantity representing the technical meaning of brightness. It is usually measured from a distance using a matrix sensor, which is the basis of the professional instrument. However, specific technical requirements must be fulfilled to achieve accurate results. This paper considers whether modern high-resolution smartphone cameras are suitable for luminance measurements. Three cameras from high-end smartphones were evaluated on a dedicated laboratory stand. The sensors' output characteristics showed relatively good linearity of the individual R, G, and B channels. Unfortunately, the spectral sensitivities were unfavorable, as the minimum error achieved was about 17%. This device is classified outside the generally accepted quality scale for photometric instruments. The presented investigation confirmed that none of the high-resolution smartphone cameras tested was suitable for use as a universal luminance camera. However, one of the test devices can be developmental if restrictively calibrated and used only in a specialistic laboratory stand. Using a smartphone (or only its camera) for luminance measurements requires proper advanced calibration. It is possible, but it limits us to only dedicated applications. The pilot study presented in this paper will help create a suitable test stand for spectacle vision systems, e.g., virtual reality equipment.

Calculated globulin can be used as a screening test for antibody deficiency in children and adolescents.

Calculated globulin (CG, total protein minus albumin levels) correlate well with IgG levels and has been proposed as a suitable screening method for individuals with primary antibody deficiencies (PADs). We aimed to show the correlation of CG with IgG levels in children and adolescents, utilizing a common method for albumin measurement, bromocresol green. Individuals from two Allergy and Immunology clinics were invited to participate. Inclusion criteria were age < 18, stable conditions, and signed informed consent. We included 1084 individuals. Immunoglobulin G values were determined by immunoturbidimetry; the colorimetric bromocresol green method and the Architect Biuret method were utilized for the albumin and total protein (TP) measurements, respectively. A total of 1084 individuals were included in the analysis and divided into 4 age groups (0 to <1 year, 1 to <4 years, 4 to <10 years, and 10 to <18 years). For all patients, the mean age was 6.1 (± 5) years old, the mean IgG was 9.4 (± 4.7) g/L, and CG was 23.7 (± 5.9) g/L. The most frequent diagnosis were respiratory allergies, followed by inborn errors of immunity. IgG and CG varied according to age group. Cutoff values for hypogammaglobulinemia varied from 13.8 g/L in children < 1 year to 23.1 g/L in children and adolescents aged 10 to <18 years. CG sensitivity varied from 70.9% in children aged 1 to <4 years old to 95.8% in children 4 to <10. Specificity ranged from 87.5% in children 4 to <10 years old to 100% in children and adolescents aged 10 to <18 years. CG is a suitable screening test for hypogammaglobulinemia in children less than 18 years of age.

Assessing the influence of actual LNG emission factors within the EU emissions trading system

The shipping sector has been incorporated into the European Union Emissions Trading System (EU-ETS). Undoubtedly, the carbon emission factor is a critical consideration when determining carbon allowances in the EU-ETS, known as EU Allowances (EUAs). In this study, an all-performance carbon emission test was conducted on a marine LNG engine to investigate the differences between the actual carbon emission factor and the reference value. It was observed that the actual carbon emission factor is smaller than the reference value. The test results indicate a discrepancy of approximately 3%, resulting in potential savings of 10,000 to 20,000 dollars in EUAs for a ship consuming 2000 tonnes of LNG fuel annually. Furthermore, variations in the carbon emission factor were observed under different engine operational conditions, fluctuating by around 2% at constant revolutions per minute (RPM). This suggests the need for more suitable emission testing methods to accurately reflect actual emissions and better achieve emission control goals.

Study on the Applicability of Deterioration Detection Techniques for Sandstone Heritage in Multi Environment using MLP-Attention Model

Abstract. Stone cultural heritage, encompassing a broad spectrum of artifacts such as stone artworks, buildings, tools, and utensils, represents one of the most significant categories of cultural heritage. However, the conservation of these cultural heritage faces challenges from the process of deterioration. This degradation not only compromises the structural integrity of the heritage but also results in the loss of invaluable historical information. Thus, there emerges a critical demand for effective methods to detect and assess the condition of stone cultural heritage, enabling timely and precise conservation interventions. Here we obtained sandstone samples under different deteriorative environments through laboratory-simulated deterioration experiments and employed a variety of detection technologies to capture a series of changes in the deterioration detection parameters during the sandstone deterioration process. Subsequently, a deep learning model was established to correlate the detection parameters with the degree of stone deterioration. A SHAP analysis was then conducted to determine the contribution of various parameters to the degree of stone weathering under different experimental environments, providing recommendations for selecting appropriate detection technologies and indicators adapted to different deterioration environments. To further analyze the deterioration processes of the stone samples, XRD analysis was conducted to observe changes in mineral composition throughout the deterioration process. SEM images were utilized to examine the changes in micro-morphology and the internal pore structure associated with deterioration. This study provides a basis for the scientific design of deterioration detection schemes by selecting the most suitable testing technology for optimal deterioration assessment under specific environmental conditions.

Inter-laboratory comparison of real-time quaking-induced conversion (RT-QuIC) for the detection of chronic wasting disease prions in white-tailed deer retropharyngeal lymph nodes.

The rapid geographic spread of chronic wasting disease (CWD) in white-tailed deer (WTD; Odocoileus virginianus) increases the need for the development and validation of new detection tests. Real-time quaking-induced conversion (RT-QuIC) has emerged as a sensitive tool for CWD prion detection, but federal approval in the United States has been challenged by practical constraints on validation and uncertainty surrounding RT-QuIC robustness between laboratories. To evaluate the effect of inter-laboratory variation on CWD prion detection using RT-QuIC, we conducted a multi-institution comparison on a shared anonymized sample set. We hypothesized that RT-QuIC can accurately and reliably detect the prions that cause CWD in postmortem samples from medial retropharyngeal lymph node (RPLN) tissue despite variation in laboratory protocols. Laboratories from 6 U.S. states (Michigan, Minnesota, Missouri, New York, Pennsylvania, Wisconsin) were enlisted to compare the use of RT-QuIC in determining CWD prion status (positive or negative) among 50 anonymized RPLNs of known prion status. Our sample set included animals of 3 codon 96 WTD genotypes known to affect CWD progression and detection (G96G, G96S, S96S). All 6 laboratories successfully identified the true disease status consistently for all 3 tested codon 96 genotypes. Our results indicate that RT-QuIC is a suitable test for the detection of CWD prions in RPLN tissues in several genotypes of WTD.

Evaluation and comparison of performances of six commercial NSP ELISA assays for foot and mouth disease virus in Thailand

ELISA kits that detect antibodies to the non-structural protein (NSP) of the foot-and-mouth disease virus (FMDV), commonly referred to as NSP-ELISA, can distinguish between vaccinated and naturally infected animals. They can play an essential role in demonstrating ‘proof-of-freedom’ during the control of FMD. Although various NSP-ELISA kits are available in Thailand, information regarding their performance is lacking. To select the most appropriate NSP-ELISA kit for our specific purpose, we must compare their performance using carefully characterized sera. This will ensure that we maximize the benefits of our testing. In this study, six NSP-ELISA kits sold in Thailand―Biovet, ID Screen, VDPro, IDEXX, PrioCHECK, and KUcheck-F―were evaluated and compared. A total of 800 serum samples were examined, including samples from 357 cattle and 29 buffaloes in outbreak areas, as well as 14 swine serum samples from the Vaccine Quality Control Unit of the Bureau of Veterinary Biologics, Ministry of Agriculture and Cooperation, Thailand. Four hundred samples were confirmed to originate from animals infected with FMDV through ELISA typing (n = 11, tested as representative samples in each farm) and/or RT-PCR (n = 400, all samples), serving as positive control sera. Additionally, 400 negative control sera were obtained from Japan (97 cattle and 300 pigs) and Australia (3 goats), certified by the World Organisation for Animal Health as ‘free of FMD’. The sensitivity and specificity of the six tests were determined based on the results obtained from two-by-two tables. Cohen’s kappa statistics were calculated for the six tests to assess their concordance, and the diagnostic accuracy of the assays was also determined. For all six NSP-ELISA kits, the sensitivity ranged from 97.75 to 99.50%, and the specificity ranged from 97.25 to 100%. Cohen’s kappa statistics ranged from 0.96 to 1.00, and diagnostic accuracy ranged from 98.13 to 99.75%. The study results indicated that the test kits have statistically similar sensitivity, specificity, concordance, and diagnostic accuracy, suggesting they can be used interchangeably. However, ID Screen demonstrated the highest sensitivity and specificity among all kits tested. Therefore, if a single kit were to be selected from the six evaluated, ID Screen would be the most appropriate choice. These findings can aid in selecting the most suitable test kit. Therefore, it is recommended to consider purchasing a diverse range of effective test kits. Furthermore, these findings can provide guidance for expanding the use of test kits, particularly with the growing availability of NSP-ELISA kits in the market.

Between Life and Death: Sea Urchin Embryos Undergo Peculiar DNA Fragmentation after Exposure to Vanadium, Cadmium, Gadolinium, and Selenium.

Exogenous DNA damage represents one of the most harmful outcomes produced by environmental, physical, or chemical agents. Here, a comparative analysis of DNA fragmentation was carried out on Paracentrotus lividus sea urchin embryos exposed to four common pollutants of the marine environment: vanadium, cadmium, gadolinium and selenium. Using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, fragmented DNA was quantified and localized in apoptotic cells mapping whole-mount embryos. This is the first study reporting how different chemicals are able to activate distinctive apoptotic features in sea urchin embryos, categorized as follows: (i) cell-selective apoptosis, showing DNA fragmentation restricted to a subset of extremely damaged cells, acting as an embryo survival mechanism; or (ii) total apoptosis, with fragmented DNA widespread throughout the cells of the entire embryo, leading to its death. Also, this is the first report of the effects of Se exposure on P. lividus sea urchin embryos. These data confirm the TUNEL assay as the most suitable test to study DNA fragmentation in the sea urchin embryo model system. Taken together, this research highlights embryos' ability to find alternative pathways and set physiological limits for development under stress conditions.

DEVELOPMENT OF WATER QUALITY MONITORING SYSTEM IN SHRIMP PONDS BASED ON INTERNET OF THINGS (IOT)

This type of research is research and development (R&amp;D) which uses the waterfall development model. The aim of this research is to produce a water quality monitoring system in shrimp ponds based on the Internet of Things (IoT) and to find out the results of testing the development of the system using the ISO 25010 standard. This development involves the application of ESP32, Relay, Toa Siren, DS18b20 Temperature Sensor, pH Sensor Module 4502C, Water Salinity and Turbidity Sensor which allows users to get real-time pond water quality monitoring information via the Telegram application. The test results for the reliability aspect have met the fault tolerance sub-characteristic standards because the percentage of measurement error obtained for the temperature sensor was 0.10%, pH 0.54%, salinity 1.19%, and turbidity 1.00%, still within the permitted tolerance limits. Functionality suitability testing shows that this tool obtains a percentage of 100%, indicating that the system is suitable for use. This tool also meets the portability aspect because it can be used on various devices with different operating systems. Apart from that, the usability evaluation results from user responses are in the very good category with an average of &gt;4.2.

Comparing the short-term leaching behaviour of cements containing supplementary cementitious materials in different leachants for low-level waste encapsulation

Pulverized fly ash (PFA) is commonly used as a supplementary cementitious material (SCM) in cement and is permitted for use in cements and grouts used for the encapsulation of low-level nuclear waste. However, with a global decrease in availability of PFA predicted in the near future, alternative SCMs are required. In nuclear encapsulation, a key parameter for a new SCM is that it does not change the leaching behaviour of the grout. We present promising initial leaching data from two SCMs: metakaolin and waste paper fly ash. These were tested for 24 h in two leachants, deionized water and an artificial groundwater, tracking the pH and leachability of a range of elements as the samples equilibrated with the leachants. The study suggests that for many elements, both metakaolin and waste paper fly ash show a very similar leaching behaviour to PFA grout formulations despite differences in the pH equilibration. In general, the tests suggest that the composition of the elute is dominated by leaching from the cementitious phases, and not the SCM. This suggests that further longer-term leaching studies are required as part of testing for SCM suitability for nuclear grouts in order to understand the connections between leaching and grout microstructure.

Negative results from DNA-based population screening for adult-onset diseases: the recipients' experience.

DNA-based population screening for adult-onset diseases holds promise for advancing personalized medicine and improving public health. Yet as most individuals pursuing such screening receive negative results, the return of results process must ensure that negative results and their implications are clearly understood. We explored the experiences of adults who received negative results from such screening as part of the Electronic Medical Records and Genomics consortium Phase 3 project (eMERGE-3) at Columbia University. In addition to a laboratory report and a standard counseling letter explaining the negative results, participants were randomized to receive (or not) a vignette explaining the results. A diverse cohort of 437 adult participants completed both baseline and post-result surveys. Many participants reported motivations that did not match the screening goals and included hope for diagnosis and family disease risk. A quarter of participants reported not feeling confident explaining their results to others (n = 105, 24%), and those who did not receive the vignette were less confident than those who did (29% versus 19% respectively; p-value = 0.02). Open-text responses about personal and family members' reactions to the results suggested that some perceived an exaggerated benefit from the negative result and might forgo more appropriate genetic testing. Our findings highlight the complexity of returning negative results and raise concerns that participants might forgo more suitable genetic testing. Future research is needed to compare the efficacy of different forms of ancillary materials on individuals' comprehension of negative results.