Sugars Glucose Research Articles

Information on semaglutide and compounding

Semaglutide belongs to a class of medications known as glucagon‐like peptide‐1 (GLP‐1) receptor agonists. It mimics the GLP‐1 hormone that is released in the gastrointestinal tract in response to eating. One role of GLP‐1 is to prompt the body to produce more insulin, which reduces blood glucose (sugar). GLP‐1 in higher amounts also interacts with the parts of the brain that reduce appetite and signal a feeling of fullness.

Enhancing glycaemic control and promoting cardiovascular health: the therapeutic potential of Trigonella foenumgraecum in diabetic patients – a systematic review and meta-analysis

Background: The antidiabetic potential of fenugreek has been highlighted in past literature, and various in-vitro and in-vivo studies have validated its glucose-lowering effects; however, very limited data are available on its effects on diabetic patients. Objective: An updated systematic review and meta-analysis of randomized control trials that assessed patients who were administered fenugreek. Methods: The PRISMA guidelines (Supplemental Digital Content 1, http://links.lww.com/MS9/A361) were followed when conducting this meta-analysis. PubMed, Scopus, Google Scholar and MEDLINE were searched from inception until June 2023, for randomized control trials that compared fenugreek with control in patients with type 2 diabetes mellitus (DM) and reported the following outcomes of interest: fasting blood glucose, glycated haemoglobin A1c (HbA1c) and postprandial glucose levels. The findings were presented as mean difference (MD) with 95% confidence intervals (CIs) and were pooled using a random effects model. Results: Fenugreek significantly (P<0.001) reduced the fasting blood sugar (FBS), HbA1c levels and postprandial glucose levels in diabetic patients when compared to the control. Conclusion: Among patients with type 2 DM, our comparisons demonstrated a reduction in FBS, HbA1c levels and postprandial glucose levels with the administration of fenugreek seed at 2–5 mg dose in powder form.

Coinoculation of Saccharomyces cerevisiae and Bacillus amyloliquefaciens in solid-state and submerged coffee fermentation: Influences on chemical and sensory compositions

This study aimed to evaluate the influence coffee fermentation with yeast and bacillus as starter cultures in SIAF method applied in solid-state and submerged process. Saccharomyces cerevisiae and Bacillus amyloliquefaciens were separately and coinoculated into natural coffee in buckets with or without water adding. The fermentations were performed for until 96 h, and the effects on physicochemical composition including the concentrations of organic acids (citric, lactic, acetic, malic), sugars (glucose, fructose, sucrose), ethanol, and glycerol as well as the sensory profiles of the resulting coffee beverages, were evaluated. All coffees produced were classified in specialty coffee with final score above 84. The coinoculated coffees obtained the highest sensory scores in submerged process (>86). Due to the results found, the type of process, submerged and solid-state, showed more influence in chemical and sensory analyses than type of inoculation. Submerged process showed major concentration of lactic acid, while solid-state process promotes major concentrations of ethanol, glycerol and acetic acid. Different sensory descriptions were observed in each treatment. Coffees from the submerged process showed nutty, cocoa, and sweet sensory descriptions, while solid-state process produced coffees with sour and acidic attributes.

Comparison of Logistic Regression, Random Forest and Adaboost Algorithms for Diabetes Mellitus Classification

Diabetes mellitus is a chronic disease that affects the way the body regulates sugar (glucose). High blood sugar levels can lead to health complications including heart problems, eye disorders, nerve damage, kidney and blood vessel disorders. It is important for early detection of diabetes by utilizing data mining technology. Data mining has various classification models that can be used to detect diabetes, including logistic regression, random forest and adaboost. The comparison of the three algorithms aims to find out which algorithm is most appropriate in the classification of diabetes. From the results obtained, the random forest algorithm has the best performance in the classification of diabetes mellitus compared to other algorithms.

Isolation, screening, and characterization of the newly isolated osmotolerant yeast Wickerhamomyces anomalus BKK11-4 for the coproduction of glycerol and arabitol.

This study explored the isolation and screening of an osmotolerant yeast, Wickerhamomyces anomalus BKK11-4, which is proficient in utilizing renewable feedstocks for sugar alcohol production. In batch fermentation with high initial glucose concentrations, W. anomalus BKK11-4 exhibited notable production of glycerol and arabitol. The results of the medium optimization experiments revealed that trace elements, such as H3BO3, CuSO4, FeCl3, MnSO4, KI, H4MoNa2O4, and ZnSO4, did not increase glucose consumption or sugar alcohol production but substantially increased cell biomass. Osmotic stress, which was manipulated by varying initial glucose concentrations, influenced metabolic outcomes. Elevated glucose levels promoted glycerol and arabitol production while decreasing citric acid production. Agitation rates significantly impacted the kinetics, enhancing glucose utilization and metabolite production rates, particularly for glycerol, arabitol, and citric acid. The operational pH dictated the distribution of the end metabolites, with glycerol production slightly reduced at pH 6, while arabitol production remained unaffected. Citric acid production was observed at pH 6 and 7, and acetic acid production was observed at pH 7. Metabolomic analysis using GC/MS identified 29 metabolites, emphasizing the abundance of sugar/sugar alcohols. Heatmaps were generated to depict the variations in metabolite levels under different osmotic stress conditions, highlighting the intricate metabolic dynamics occurring post-glucose uptake, affecting pathways such as the pentose phosphate pathway and glycerolipid metabolism. These insights contribute to the optimization of W. anomalus BKK11-4 as a whole-cell factory for desirable products, demonstrating its potential applicability in sustainable sugar alcohol production from renewable feedstocks.

Formulation and Evaluation of Lip Balm using Tomato Extract to Select the Best Concentration of Base

Solanum lycopersicum, known as tomato, is a perennial plant with a weak body and can grow to a height of 1-3 m. It has a yellow flower that grows to be a tomato [1]. Tomato contains 93-95% water, and the remaining constituents include 5-7% inorganic compounds, sugars (glucose, sucrose, and fructose), organic acids (citric acid malic), solids insoluble in alcohol (proteins, pectin, cellulose, and polysaccharides), lipids and carotenoids[2]. Besides, phytochemicals such as alkaloids, flavonoids, glycosides, saponins, tannins, steroids, phlorotannins, and terpenoids were found in both aqueous and methanolic tomato extracts [3,4]. Tomato also contains antioxidants such as vitamins C and (, ȕ carotene, lycopene, lutein, and flavonoids [5].

Correlation Between Thrombocyte Amount and Transient Blood Glucose to Diabetic Foot Ulcers

Objectives: Diabetes Mellitus (DM) is a disease that is caused by an increase of blood sugar glucose, this condition have potential to increase the damage of macrovascular and microvascular. One of the main complications of Diabetes Mellitus Type II (DMT2) is diabetic foot ulcer. Hyperglycemia in T2DM is thought to trigger neuropathy which can cause diabetic foot ulcers and also associated with an increase of thromboxane A2 (vasoconstrictor and platelet aggregator agonist), thereby increasing the risk of plasma hypercoagulability. Methods: The research design at this research is analytic descriptive with a cross-sectional study approach and involves 21 patient with T2DM with diabetic foot ulcers. The level of Transient Blood Glucose (TBG) and thrombocyte was examined from venous blood. Results: This research showed that there were increased thrombocytes in 6 patients (28.57%); increased transient blood glucose in 17 patients (80.95%); and increased transient blood glucose and thrombocytes at the same time were seen in 5 patients (23.8%). Correlation between TBG to diabetic foot ulcer (p=0.236 < 0.05); the correlation between thrombocyte to diabetic foot ulcer (p=0.112 <0.05); the correlation between TBG and thrombocyte to diabetic foot ulcer (r=-0.372, p=0.097), the correlation between TBG to thrombocyte (r=-0.309, p=0.228). Conclusion: From these results we might conclude that there is no relationship between TBG to diabetic foot ulcer, no relationship between thrombocyte to diabetic foot ulcer, weak relationship between TBG and thrombocyte, and there is no relationship between high TBG to thrombocyte. But we cannot exclude other factors that might have influenced the results and requires more research for a more conclusive outcome.International Journal of Human and Health Sciences Supplementary Issue 01: 2024 Page: S59

High sugar consumption for seven days in adult mice increased blood glucose variability, induced an anxiolytic effect and triggered oxidative stress in cerebral cortex.

Brain function is highly altered by glucose toxicity related to diabetes. High consumption of sugar in normal conditions is suspected to affect as well brain integrity. The present study investigates the possible effects of short-term exposure to high sugar diet on brain redox homeostasis in healthy mice. Male adult healthy mice were divided into two groups: control (CG) and sugar-exposed group (SG), that was exposed continually to 10% of glucose in drinking water for 7 days and 20% sucrose pellets food. Behavior, blood glucose variability and cerebral cortex oxidative stress biomarkers were measured at the end of exposure. Animals exposed to the high sugar diet expressed a significant increase in blood glucose levels and high glucose variability compared to control. These animals expressed as well anxiolytic behavior as revealed by the plus maze test. Exposure to the sugar diet altered redox homeostasis in the brain cortex as revealed by an increase in lipid peroxidation and the activity of antioxidant enzymes superoxide dismutase (SOD) and glutathione-s-transferase (GST). On the other hand, catalase (CAT) activity was decreased, and reduced glutathione (GSH) level was not altered compared to control. Further studies are required to understand the mechanisms trigging oxidative stress (OS) in the brain in response to short term exposure to high sugar diet and glucose fluctuations.

Red LED light affects the physicochemical responses of strawberries during storage

This study aimed to evaluate the effect of the storage of strawberries under LED lights on ‘Albion’ strawberry quality. The treatments were applied as follows; (1) storage under continuous blue, red, and ultraviolet-A (UVA) LED light, (2) storage in the dark conditions (control), and (3) storage in the dark conditions after 1 h UVA (UVAh) LED lighting. Strawberries were stored at a temperature of 4±1oC with 85-90% relative humidity for 10 days. In the study, analyses were conducted on the total anthocyanin content, color (L*, hue angle, redness index), total soluble solids (TSS), fructose, glucose, total sugar content, titratable acidity (TA), fruit firmness (N), and weight loss at the start of the experiment and at 2-day intervals during storage. According to the results, the storage of strawberries under continuous red-LED light was successful in improving the anthocyanin and TSS contents, while preserving fruit firmness and reducing weight loss. Moreover, UVA treatment was effective in maintaining the L*, a*, and b* color values, whereas UVAh was effective on the hue angle and redness index. Furthermore, UVAh treatment caused a decrease in the glucose, fructose, and total sugar content and, in the titratable acidity of the strawberries.

Kegiatan Deteksi Dini Penyakit Diabetes Mellitus Tipe 2 melalui Pemeriksaan Gula Darah Sewaktu pada Kelompok Usia Produktif

Diabetes mellitus (DM) occurs when blood sugar (glucose) levels are very high for a long period of time. If the glucose level is less than 126 mg/dL during fasting or less than 200 mg/dL two hours after eating, we diagnose diabetes. In low- and middle-income countries, DM affects as many as 422 million people and is one of the leading causes of death worldwide. Uncontrolled diabetes can cause severe complications such as cardiovascular disease, kidney damage, nerve damage, and vision problems. To prevent this complication, early detection through blood glucose checks is critical. This activity uses the PDCA (Plan-Do-Check-Act) method for diabetes screening in adults at PT. Narindo, North Jakarta. The results of blood sugar checks showed that 5 people (3.97%) had blood sugar levels above normal. Increasing public awareness and knowledge about the risks of diabetes and healthy living is very important to reduce the incidence of diabetes so that it can improve overall health outcomes.

Triblock Glycopolymers with Two 10-mer Blocks of Activating Sugars Enhance the Activation of Acrosomal Exocytosis in Mouse Sperm.

Carbohydrate recognition is imperative for the induction of sperm acrosomal exocytosis (AE), an essential phenomenon in mammalian fertilization. In mouse sperm, polynorbornene 100-mers displaying fucose or mannose moieties were effective at inducing AE. In contrast, glycopolymers exhibiting glucose sugars resulted in no AE activation. To further elucidate the role of ligand density on the activation of AE in mouse sperm, a triple-stain flow cytometry assay was employed to determine the efficacy of polynorbornene block copolymers with barbell-like sequences as initiators of AE. Triblock (ABA or ABC) copolymers were synthesized by ring-opening metathesis polymerization (ROMP) with one or two activating sugars, mannose or fucose, and one nonactivating sugar, glucose. The active ligand fractions in the polymers varied from 10, 20, or 40%. Simultaneously, random copolymers comprising 20% activating ligands were prepared to confirm the importance of ligand positionality in AE activation in mouse sperm. Polynorbornene 100-mers possessing two 10-mer blocks of activating sugars were the most effective copolymers at inducing AE with levels of AE comparable to their homopolymer counterparts and more effective than their random analogues. Small-angle X-ray scattering (SAXS) was then performed to verify that there were no differences in the conformations of the glycopolymers contributing to their varying AE activity. SAXS data analysis confirmed that all of the glycopolymers assumed semiflexible cylindrical structures with similar radii and Kuhn lengths. These findings suggest that the overall ligand density of the sugar moieties in the polymer is less important than the positionality of short blocks of high-density ligands for AE activation in mouse sperm.

Impact of immature coffee fruits and water addition during spontaneous fermentation process: Chemical composition and sensory profile

BackgroundCoffee fermentation process influences the final coffee composition and the sensory aspects which define the quality of the beverage. In this study, coffee fruits underwent spontaneous self-induced anaerobic fermentation using samples with two percentages of immature fruits in submerged and solid-state processing. The effects on the physicochemical composition and sensory quality of coffees were evaluated. ResultsThe two percentages of immature fruits corresponded to 11.0 and 0.3% of unripe fruits. The percentage of immature fruits significantly altered the initial content of sugars (sucrose, glucose, and fructose), ash, and titratable acidity. The water addition during the fermentative process did not significantly influence final moisture, proteins, citric acid, and propionic acid concentrations. Compared to the solid-state, the submerged process gave rise to coffees with lower concentrations of ethanol, glycerol, ash, lipids, succinic, and acetic acids. Coffee fermented with 0.3% of immature fruits showed higher lactic acid production in submerged fermentation (67.44 mg/g), and higher concentrations of ethanol (42.84 mg/g) and glycerol (1.68 mg/g) in solid-state fermentation. All coffees produced were classified as specialty coffees with a score above 84 points. However, the submerged fermented coffee with 11% immature fruit stood out with notes of caramel, brown sugar, honey, orange, lemon, floral, nut, yellow and red fruits. ConclusionsThis study confirmed that spontaneous fermentation can be used to produce specialty coffees. Differentiation in sensory attributes can be achieved through the addition of water and varying the percentage of green fruits during the fermentation process. Up to 11% of immature fruits did not compromise coffee quality.How to cite: Ferreira LJC, Casé IN, Bertarini PLL, et al. Impact of immature coffee fruits and water addition during spontaneous fermentation process: Chemical composition and sensory profile. Electron J Biotechnol 2024;69. https://doi.org/10.1016/j.ejbt.2024.04.001.

The antiglycation potential of H1 receptor antagonists – in vitro studies in bovine serum albumin model and in silico molecular docking analyses

The H1 receptor belongs to the family of rhodopsin-like G-protein-coupled receptors activated by the biogenic amine histamine. H1 receptor antagonists are widely used in the treatment of allergies. However, these drugs could have a much broader spectrum of activity, including hypoglycemic effects, which can broaden the spectrum of their use. The aim of the study was to evaluate the antiglycation potential of twelve H1 receptor antagonists (diphenhydramine, antazoline, promethazine, ketotifen, clemastine, pheniramine, cetirizine, levocetirizine, bilastine, fexofenadine, desloratadine, and loratadine). Bovine serum albumin (BSA) was glycated with sugars (glucose, fructose, galactose, and ribose) and aldehydes (glyoxal and methylglyoxal) in the presence of H1 blockers. The tested substances did not induce a significant decrease in the content of albumin glycation end-products, and the inhibition rate of glycoxidation was not influenced by the chemical structure or generation of H1 blockers. None of the tested H1 receptor antagonists exhibited strong antiglycation activity. Antiglycemic potential of H1 blockers could be attributed to their antioxidant and anti-inflammatory activity, as well as their effects on carbohydrate metabolism/metabolic balance at the systemic level.

Valorization of Chlorella Microalgae Residual Biomass via Catalytic Acid Hydrolysis/Dehydration and Hydrogenolysis/Hydrogenation

Microalgal biomass can be utilized for the production of value-added chemicals and fuels. Within this research, Chlorella vulgaris biomass left behind after the extraction of lipids and proteins was converted to valuable sugars, organic acids and furanic compounds via hydrolysis/dehydration using dilute aqueous sulfuric acid as a homogeneous catalyst. Under mild conditions, i.e., low temperature and low sulfuric acid concentration, the main products of hydrolysis/dehydration were monomeric sugars (glucose and xylose) and furanic compounds (HMF, furfural) while under more intense conditions (i.e., higher temperature and higher acid concentration), organic acids (propionic, formic, acetic, succinic, lactic, levulinic) were also produced either directly from sugar conversion or via intermediate furans. As a second valorization approach, the residual microalgal biomass was converted to value-added sugar alcohols (sorbitol, glycerol) via hydrogenation/hydrogenolysis reactions over metallic ruthenium catalysts supported on activated carbons (5%Ru/C). It was also shown that a low concentration of sulfuric acid facilitated the conversion of biomass to sugar alcohols by initiating the hydrolysis of carbohydrates to monomeric sugars. Overall, this work aims to propose valorization pathways for a rarely utilized residual biomass towards useful compounds utilized as platform chemicals and precursors for the production of a wide variety of solvents, polymers, fuels, food ingredients, pharmaceuticals and others.

Smart-Watches Assisted Sugar Level Monitoring with Different Activities and Nutrition based on Machine Learning Approaches

These days, sugar glucose monitoring is very important for both diabetic and non-diabetic patients while they are eating and doing different activities in practice. There are different ways to monitor body glucose levels such as blood-based glucose monitoring and smart watches-based glucose monitoring. However, continuous glucose monitoring (CGM) is an emerging non-invasive method for different subjects (e.g., patients and customers). However, smartwatches have limitations. In this paper, we present a new smartwatch framework that monitors the body's glucose level with new features such as nutrition, and activities. We present the modified dataset with an additional feature such as sugar glucose level with different activities (e.g., running, sitting, sleeping, and walking) while eating different nutrition in different time intervals. We present empirical machine learning such as an activity glucose monitoring algorithm (ASA) which executes all datasets with more optimal results. Simulation results show that our proposed framework is more optimal and shows glucose monitoring with different activities with more features as compared to existing smartwatches and obtained an accuracy of 78% as compared to existing machine learning methods.

Textural formation of instant controlled pressure drop–dried peach chips: Investigation of the electrical, thermal, and textural properties of predried peach slices with osmotic dehydration pretreatment

In this study, the effect of osmotic dehydration (OD) pretreatment with various sugar (erythritol, glucose, and trehalose) on the quality of hot-air-predried peach slices was investigated, particularly focusing on electrical properties, texture, thermal stability, and cell wall strength. Furthermore, the correlation between the properties of predried peach slices and the texture of the instant controlled pressure drop (DIC) dried peach chips was explored. OD pretreatments improved the stability and integrity of the cell wall and cell membrane of pre-dried peach slices, which inhibited the excessive expansion of samples during DIC drying. Especially, peach chips with trehalose-OD exhibited the highest crispiness (1.05 mm), the highest hardness (101.34 N) was obtained in erythritol-OD samples. Overall, the type of osmotic agents affected the texture of DIC peach chips with OD pretreatments. It should be noted that trehalose is a promising osmotic agent for controlling and regulating the quality of DIC peach chips.

Selective extraction of high-added value carboxylic acids from aqueous fermentative effluents with new hydrophobic eutectic solvents (HES)

SummaryRecovering these carboxylic acids from the fermentative streams in a sustainable, green, and economical way is a significant challenge. This work assessed hydrophobic eutectic solvents (HES) – water-immiscible – for the selective recovery of carboxylic acids via liquid–liquid extraction. Different trioctylphosphine oxide (TOPO) mixtures with menthol and thymol were studied and deeply characterized by 1H and 31P NMR to yield stable eutectic solvents, including a novel experiment of 31P NMR at variable temperatures for the first time. Those stable eutectic solvents were tested in the liquid extraction of complex aqueous mixtures containing C2–C6 carboxylic acids and simple sugars (glucose and xylose). The back-extraction of the carboxylic acids for the recovery of the HES was optimized, being necessary in three stages for the complete cleaning of the eutectic solvent using NaOH 0.1 M. The eutectic mixture of TOPO and thymol in a molar ratio of 1:2 exhibited an overall recovery of C5 and C6 carboxylic acids over 70 %, allowing its selective extraction from the rest of the compounds in the complex mixture. Likewise, this HES (after back extraction) was successfully reused in a second extraction cycle, keeping the performance of the fresh one. Therefore, this study demonstrated that HES can have a high extraction selectivity for carboxylic acids of ≥C5. Moreover, these solvents were stable and allowed reusability, reducing the environmental impact and process costs.

Tunable Q-factor wavelet transform based identification of diabetic patients using ECG signals

Diabetes is a chronic health condition that is characterized by increased levels of glucose (sugar) in the blood. It can have harmful effects on different parts of the body, such as the retina of the eyes, skin, nervous system, kidneys, and heart. Diabetes affects the structure of electrocardiogram (ECG) impulses by causing cardiovascular autonomic dysfunction. Multi-resolution analysis of the input ECG signal is utilized in this paper to develop a machine learning-based system for the automated detection of diabetic patients. In the first step, the input ECG signal is decomposed into sub-bands utilizing the tunable Q-factor wavelet transform (TQWT) technique. In the second step, four entropy-based characteristics are evaluated from each SB and elected using the K-W test method. To develop an automatic diabetes detection system, selected features are given as input with 10-fold validation to a SVM classifier using various kernel functions. The 3 rd sub-band of TQWT with the Coarse Gaussian kernel function kernel of the SVM classifier yields a classification accuracy of 91.5%. In the same dataset, the comparative analysis demonstrates that the proposed method outperforms other existing methods.

Abscisic acid treatment prolongs the postharvest life of strawberry fruit by regulating sucrose and cell wall metabolism

After harvesting, strawberry fruit (Fragaria × ananassa, Duch.) are highly susceptible to deterioration in appearance, firmness and quality, all of which limit storage and transportation life. To increase storage life and reduce losses of strawberry, a pre-storage spray treatment of ripe fruit with relatively low concentrations (100 μM) of abscisic acid (ABA) were used. ABA treatment increased the concentration of the soluble sugars sucrose and glucose, and modified the expression of genes involved in sugar metabolism with a substantial reduction in the mRNA abundance of a highly-expressed neutral invertase. ABA treatment also suppressed the expression of polygalacturonase and a pectate lyase that have been shown to be involved in strawberry fruit softening. Corresponding improvements in middle lamella structure and intercellular adhesion were observed using microscopy. The results show that ABA extended the postharvest life of strawberries by increasing content of soluble sugars and maintaining integrity of cell wall.

Effects of phosphorus and potassium fertilization and fruit canopy position on sugar accumulation in mangifera indica cv. 'Kent' pulp

Sugar content is the key to mango quality and growers are constantly seeking methods to optimize it. In this study, fructose, glucose, sucrose, and maltose sugars were evaluated to determine how phosphorus and potassium fertilizers in varying amounts could affect these sugar levels and how sunlight exposure effects fruit. The experiments were conducted in the KALRO Orchard of Kiambu County, Kenya. Thirty-five healthy 7- to 8-year-old ‘Kent’ trees were selected and divided into five treatment groups with five replicates each. Treatment with 1 kg, 2 kg P, K and combined PK per tree resulted in a significantly higher fructose content than the control. In contrast, neither 1 kg nor 2 kg P resulted in significantly higher glucose contents than the control. Combined PK fertilizers at 0.5+0.5 kg per tree reduced fructose and increased glucose, sucrose, and maltose. A combination of PK fertilizers at 1.0+1.0 kg significantly increased only fructose, but not other sugars. Even after applying P and K fertilizers, the position of the fruit canopy did not affect fructose, glucose, or maltose contents. However, there was a trend towards a higher sucrose content in fruits exposed to sunlight (5.17 g/100 g) than in canopy fruits (2.29g/100g), although this difference was not statistically significant. The same trend was observed for the other sugars. The t-test revealed no significant differences between the two canopy positions (t = 1.01, p = 0.344). A significant difference in sugar content (p < 0.05) between fruits harvested at different canopy positions suggests that fruits harvested at different canopy positions did not ripen simultaneously. In contrast, neither 1 kg nor 2 kg of P produced a significant difference in the glucose content. Combining PK fertilizers at 0.5+0.5 kg per tree decreased fructose while increasing glucose, sucrose, and maltose. A combination of PK fertilizers at a rate of 1.0+1.0 kg significantly increased only fructose but not other sugars. Even after the application of P and K fertilizers, the position of the fruit canopy did not affect fructose, glucose, and maltose contents. However, there was a trend towards a higher sucrose content in fruits exposed to sunlight (5.17 g/100 g) than in crown fruits (2.29 g/100 g), although this difference was not statistically significant. The same trend was observed for the other sugars. The t-test revealed no significant differences between the two canopy positions (t = 1.01, p = 0.344). A significant difference in sugar content (p< 0.05) between fruits harvested at different canopy positions suggests that fruits harvested at different canopy positions did not ripen simultaneously.