Sugarcane Seeds Research Articles

Production and performance of sugarcane seeds (caryopses) from different hybridizations involving RB92579.

A major challenge in sugarcane breeding program is the obtaining of enough number of seeds (caryopses) for the development of new improved cultivars. Genotypes differ in their function as pollen recipient and pollen donor, which also affect the seed performance. Thus, the aim of this study was to verify the production and performance of sugarcane seeds from different hybridizations involving RB92579 as pollen recipient and pollen donor. Twelve bi-parental crossings were carried out involving RB92579 and other different genotypes randomly chosen. Seed production potential was evaluated by percentage of fertile spikelets and caryopsis fresh weight. The seed physiological potential was determined by evaluating germination and vigor (index of germination rate, number of normal seedlings per gram of fuzz, and seedling dry weight). The results showed better performance for RB92579 as pollen donor for all characteristics studied. Therefore, RB92579 sugarcane cultivar should be used as pollen donor during hybridizations, condition that permits a greater production and physiological performance of seeds for the sugarcane breeding programs.

Effect of Planting Materials and Integrated Nutrient Management on Yield of Sugarcane Seed Crop

A field experiment was conducted at Sugarcane Research Station, Cuddalore during special season of 2017 - 18 (July - February) to select an appropriate planting material of sugarcane seed crop and to ascertain the effective integrated nutrient management package to enhance the productivity of the same. The sugarcane variety used was CoC 25. The experiment was laid out in split plot design and the treatments were replicated thrice. The treatments comprised of three different sugarcane planting materials viz., double budded setts (S1 ) , single bud setts (S2 ) and chip bud seedlings (S3 ) were allocated to the main plots and four integrated nutrient management practices viz., 100 per cent recommended dose of NPK (N1 ), 75 per cent of recommended NPK + Azophos + insitu incorporation of sunnhemp (N2 ), 75 per cent of recommended NPK + insitu incorporation of sunnhemp + foliar spraying of sugarcane booster (N3 ), 75 per cent of recommended NPK + Azophos + insitu incorporation of sunnhemp + foliar spraying of sugarcane booster (N4 ) were accommodated in sub plots. Planting of chip bud seedlings recorded higher establishment percentage (87.01), plant height (229.46 cm), tiller population ( 113.01 ‘000 ha-1), dry matter production (85.87 t ha-1), single cane weight (1.80 kg/cane), seed cane yield (98.32 t ha-1), gross return (Rs. 2,94,962 / ha), net return (Rs. 1,83,040) and B:C ratio (2.63). Among integrated nutrient management practices, application of 75 per cent of recommended NPK + Azophos + insitu incorporation of sunnhemp + foliar spraying of sugarcane booster resulted in higher establishment percentage (78.20), plant height (217.06 cm), tiller population (105.45 ‘000 ha-1), dry matter production (86.77 t ha-1), single cane weight (1.59 kg/cane), seed cane yield (88.71t ha-1), gross return (Rs. 2,66,138 / ha) net return (Rs. 1,56,819) and B:C ratio (2.42).

Effect of Sugarcane Trash Biochar on Growth and Yield of Sugarcane Seed Crop

A field experiment was conducted at Sugarcane Research Station, Cuddalore with the objective to ascertain the effect of sugarcane trash biochar on growth and yield of sugarcane seed crop during special season 2017- 2018. The variety used was CoC 25 and the treatment consists of Recommended NPK alone (300:100:200 kg ha-1), Recom. NPK + sugarcane trash Bio-char @ 2 t ha-1, 50 per cent Recom. NPK + sugarcane trash Bio-char @ 2 t ha-1+ Bio-fertilizer @ 10 kg ha-1, 75 per cent Recom. NPK+ sugarcane trash Bio-char @ 2 t ha-1 + Bio fertilizer @ 10 kg ha-1, 100 per cent Recom. NPK + sugarcane trash Bio-char @ 2 t ha-1 + Bio-fertilizer @ 10 kg ha-1, 125 per cent Recom. NPK + sugarcane trash Bio-char @ 2 t ha-1 + Bio fertilizer @ 10 kg ha-1, 150 per cent Recom. NPK + sugarcane trash Bio-char @ 2 t ha-1 + Bio fertilizer @ 10 kg ha-1. Integrated Nutrient Management practice of 150 per cent Recom. NPK + sugarcane trash Bio-char @ 2 t ha-1 + Bio-fertilizer @ 10 kg ha-1 recorded higher growth and yield attributes viz., plant height, tiller population, root length, dry matter production, single cane weight and seed cane yield. This was followed by 125 per cent Recom. NPK + sugarcane trash Bio-char @ 2 t ha-1 + Bio fertilizer @ 10 kg ha-1. But, higher benefit cost ratio was obtained with the application of 100 per cent Recom. NPK + sugarcane trash Bio-char @ 2 t ha-1 + Bio-fertilizer @ 10 kg ha-1

Sugarcane Bud Chip Encapsulation for ex vitro Synthetic Seed Formation

Altough the sugarcane crop had a huge world importance the planting system stil the same since the development, needing changes to increase the procution potential. So, the objective of this study was to assess the effect of sugarcane bud chip encapsulation on the initial growth of seedlings. To provide informations for a new planting system, using small stem pieces of sugarcane to produce the seedlings. Two experiments were conducted in a completely randomized design. In the first, bud chip encapsulation was assessed with six concentrations of sodium alginate (0, 10, 20, 30, 40, and 50 g L-1) cross-linked with 300 mM calcium chloride, with the encapsulated chips being kept in a greenhouse. In the second experiment, the capsules resulting from the different sodium alginate concentrations were tested for the dry mass adhered to the bud chip, moisture, swelling index, biodegradability, and solubility. Emergence greater than 70% was obtained at sodium alginate concentrations of 0, 10, and 20 g L-1. The 30, 40, and 50 g L-1 concentrations inhibited seedling emergence and initial growth; however, when the capsule was removed, the bud chips formed viable seedlings. Encapsulation inhibited emergence because the capsule acts as a physical barrier; however, encapsulation may be used for bud chip preservation. The study of new capsules and encapsulation methods may enable the ex vitro production of synthetic sugarcane seeds.

FINANCING BEHAVIOR OF THE PLANT OF SUGARCANE TO FARMERS IN BLITAR DISTRICT (Ethno methodology Study on Sugarcane Farmers in Rejoso Village, Binangun District, Blitar District)

This research was conducted in Rejoso village, Binangun sub-district, Blitar district, with research object of sugar cane farmers there. This study aims to find out how the behavior of farmers in Rejoso Village about financing in the management of sugar cane, especially in financing behavior of the plant of sugarcane. In Rejoso village, plant of sugarcane is only done once in the period of 5 to 10 years because once planting sugarcane can be harvested many times even up to 10 times the harvest. The type of research used is qualitative research using Ethnomethodology method. Ethnomethodology is a study of how people create and understand their everyday life and the way they complete daily life. The subject for ethno methodology is not primitive tribal people but people of various situations in our own society. In this study, the subject of research is the sugar cane farmers in the Rejoso Village, Binangun District, Blitar. Data obtained by interview technique then result of the interview then analyzed to know how financing behavior of the plant of sugarcane. Based on the research results obtained from analyzing the results of interviews with sugar cane farmers in Rejoso Village that the financing behavior of the plant of sugarcane is vary depending on each individual. The difference in behavior lies in how to obtain sugar cane seeds, how to prepare the land and how to plant seeds of sugar cane.

FINANCING BEHAVIOR OF THE PLANT OF SUGARCANE TO FARMERS IN BLITAR DISTRICT (Ethno methodology Study on Sugarcane Farmers in Rejoso Village, Binangun District, Blitar District)

This research was conducted in Rejoso village, Binangun sub-district, Blitar district, with research object of sugar cane farmers there. This study aims to find out how the behavior of farmers in Rejoso Village about financing in the management of sugar cane, especially in financing behavior of the plant of sugarcane. In Rejoso village, plant of sugarcane is only done once in the period of 5 to 10 years because once planting sugarcane can be harvested many times even up to 10 times the harvest. The type of research used is qualitative research using Ethnomethodology method. Ethnomethodology is a study of how people create and understand their everyday life and the way they complete daily life. The subject for ethno methodology is not primitive tribal people but people of various situations in our own society. In this study, the subject of research is the sugar cane farmers in the Rejoso Village, Binangun District, Blitar. Data obtained by interview technique then result of the interview then analyzed to know how financing behavior of the plant of sugarcane. Based on the research results obtained from analyzing the results of interviews with sugar cane farmers in Rejoso Village that the financing behavior of the plant of sugarcane is vary depending on each individual. The difference in behavior lies in how to obtain sugar cane seeds, how to prepare the land and how to plant seeds of sugar cane.

The Effect of Two Different Agro Climatic Conditions on Growth and Yield Performance of Sugarcane Genotypes

Sixteen (16) Sugarcane genotypes response were studied in an experiment “genotype x location interactions” in two different agro-ecological zones (Mardan and Harichand) of Khyber Pakhtunkhwa, Peshawar-Pakistan during 2011-12 and 2012-13. The experiment was laid out in triplicate Randomized Complete Block (RCB) , design with plot size of 67 m 2 (10 m × 6.7 m). Combined analysis of variance was used to identify the presence of genotype x location interactions from replicated multi-environment trials. The data revealed significant variations among the genotypes for percentage germination, tillers, plant height, nodes plant -1 , internode length and cane yield at 1% probability level. It was observed that none of the genotypes could show superiority with respect to all attributes. However, the mean performance over locations and years indicated that the check genotype Mardan-93 remained superior by showing maximum buds germination of 42.26%. The maximum number of tillers (259.17), plant height (189.41 cm), nodes plant -1 (22.23), internode length (16.97 cm), cane diameter (23.29 mm), cane yield (68.42 t / ha), millable canes (87.83), sugar recovery (10.57%) and sugar yield (7.07 t / ha) was recorded for genotypes MS91CP238, MS99HO391, Hoth127, MS94CP15, S97CP288, MS99HO317, MS92CP979, MS91CP272, MS99HO93, MS99HO93, MS99HO93 and MS99HO317, respectively against check genotypes. It was concluded that genotypes MS99HO317, MS99HO93, MS92CP979 and MS91CP238 were superior at SCRI, Mardan (test location-I) based on tillering ability, milliable canes, cane yield, sugar recovery and sugar yield. Sugarcane Seed Multiplication Farm (SSMF), Harichand-Charsadda. (test location-II), the cultivars MS91CP272, MS99HO391, MS94CP15 and MS99HO391 were superior based on tillers, milliable canes, sugar recovery and sugar yield compared to other genotypes. The combined over years and locations performance exhibited that the genotypes MS99HO317, MS91CP238, MS92CP979 and CP89831 were superior in terms of percentage germination, tillers, cane yield, number of millable canes, sugar recovery and sugar yield.

Detecting sugarcane borer diseases using support vector machine

Based on the fact that great labor of artificial selection was needed after the sugarcane seeds were cut by the sugarcane cutting machine, and there was a misjudgment of the sugarcane borer diseases. SVM (support vector machine) method was proposed in this study to detect the sugarcane borer diseases. With the machine vision technology, together with threshold segmentation, filling and corrosion operation to process the three images of the same sugarcane whose interval is 120°. The classification features, minimum average gray value and the corresponding minimum gray value were selected by adaptive threshold segmentation algorithm, and removed the region which area of 1. The study used radial basis function as the kernel function of SVM, and roughly selected the range of regularization parameters of C and kernel function parameter σ. Finally, it selected the optimal parameters by the grid search and the cross validation method to identify sugarcane with diseases. The test showed that correct rate of diseases and disease-free sugarcane is 96%, 95.83% for the test set, so the method can effectively complete the sugarcane borer diseases detection.

Reconversión del sistema regional de producción de semilla de caña para la agroindustria panelera en Boyacá y Santander

En Colombia, la caña de azúcar Saccharum officinarum L. (Poaceae) es cultivada en 474.559 hectáreas, de las cuales 218.000 están dedicadas a la producción de azúcar y etanol y 266.559 a la producción de panela (azúcar no centrifugada). La hoya del río Suárez, en los departamentos de Boyacá y Santander, es la principal zona productora de panela de Colombia, con aproximadamente 42.701 hectáreas. Al igual que las demás regiones, esta no cuenta con un sistema de producción de caña semilla que asegure la calidad fitosanitaria y la pureza varietal que requiere el subsector, lo cual genera diseminación de plagas y enfermedades que limitan permanentemente los rendimientos. El objetivo de este trabajo fue reactivar el sistema de producción local de caña semilla en la hoya del río Suárez y para tal fin se estableció (en el marco del Plan Nacional de Semilla) el proceso de obtención de plantas germinadas mediante la extracción de yemas. Este procedimiento fue desarrollado en Cenicaña y llegó a convertirse en el sistema base para generar la caña semilla seleccionada de los productores semilleristas de la región. Durante 2014 se produjeron 200.000 plántulas que fueron entregadas a cinco asociaciones de pequeños y medianos productores. Se establecieron 15 ha de semilleros comerciales, para renovar cerca de 10.000 ha con las nuevas variedades de caña en los próximos cuatro años.

Magnitude of Heritability and Characters Association in Selected Population of Sugarcane Germplasm Under Alkaline Condition

An experiment was conducted on two groups of sugarcane accessions 15 old (out of cultivation) and 15 new (under cultivation) at the farm of Sugarcane Research and Seed Multiplication Centre, Katya Sadat, Gazipur, U.P. (India) during three consecutive autumn planting seasons 2009–2011 to 2011–2013. The mean value of the characters revealed improvement in different traits which ranged from 8.77 to 56.87 % selection differential rate. High to moderate coefficient of variance was observed for germination, number of shoots, number of millable canes, stalk height, stalk thickness, stalk weight, and cane yield in both the populations. The values of genotypic and phenotypic correlation were higher in new varieties than the old ones for each trait. Further, the value of genotypic correlation was also higher than the phenotypic correlation in both the populations for respective characters. Heritability and correlation response are important criteria for the selection of strong parentage either from the old or new population. Such combinations may be helpful in increasing the cane yield as well as sugar content in the stalk, especially in alkaline condition. The selection of potential parents from the newer population may be more beneficial than the older one except for the selection for some specific desired traits present in the old population. The entries namely CoSe 98231, CoSe 92423, CoSe 95422, CoSe 96436, BO 91, CoLk 8102, and UP 9530 were found better than other entries tested. So it may be suggested that these varieties may be used as parentage in future breeding program to evolve the genetically superior line, which may combine cane yield along with sugar content for alkaline condition area.

Diversity of the Germplasm of <i>Saccharum</i> Species and Related Genera Available for Use in Directed Breeding Programmes for Sugarcane Improvement

Sugarcane is primarily a crop raised by sett cuttings. After the discovery of fertility in sugarcane seeds, attempts have been made for its improvement through concerted breeding efforts. The first phase was limited to crossing among Saccharum officinarum clones and the resulted hybrids although had high sucrose content, lower fibre, lacked vigour, ratooning ability and resistance to diseases. In the second phase after the realization of adaptability to diverse environs, resistance to insect pests and diseases, and tolerance to abiotic stress and ratooning ability in the indigenous canes ( S. barberi, S. spontaneum and S. robustum ), a limited number of these was used in the crossing and subsequent nobilization for varietal improvement. However, the limited use of germplasm could not sustain the challenges to the crop, so also the pace of varietal development. Realization of the fact that further incorporation of S. spontaneum germplasm in breeding sporadic efforts paid dividends, attempts have been made to look into the diversity for traits of agronomic interest not only in Saccharum species, but also in the Saccharum complex, i.e. in the related genera. The present study reviews the diversity available for agronomic traits in Saccharum species clones and related genera which could be made available for use in directed breeding programmes for sugarcane improvement for the ever-increasing need of not only of sugar but also of the energy, paper and other valueadded products from sugarcane.

Response of Sugarcane Seed Plot to Microbial Inoculation by Gluconacetobacter diazotrophicus and Phosphate-Solubilizing Bacteria

A field experiment was conducted for three consecutive years to study the effect of set treatment of Gluconacetobacter diazotrophicus and PSB on seed yield, quality and saving of chemical N and P fertilizers for sugarcane seed plot. The set inoculation of Gluconacetobacter diazotrophicus + PSB coupled with 75 % recommended N and 75 % recommended P2O5 significantly improved the growth, two eye bud-set yield and quality. Among fertilizer levels, 75 % recommended N and 75 % recommended P recorded the highest yield of two eye bud-sets (883.63 and 885.44 thousands/ha, respectively). Between the inoculation treatments, fresh planting material inoculated with Acetobacter recorded higher two eye bud-sets (858.96 thousands/ha) than the uninoculated planting material from previous-year Acetobacter inoculated plot (828.47 thousands/ha); however, they were statistically indistinguishable. Moreover, the higher population and activity of G. diazotrophicus were observed at 75 % recommended N and 75 % recommended P. Among various interactions, the interaction of 75 % N and inoculation treatment showed significantly highest Acetobacter count in cuttings (7.38 × 104). The available N and P status of soil at harvest was significantly influenced by different fertilizer levels (N and P levels), inoculation treatments and their interactions as well.

Poor Fertility, Short Longevity, and Low Abundance in the Soil Seed Bank Limit Volunteer Sugarcane from Seed.

The recent development of genetically modified sugarcane, with the aim of commercial production, requires an understanding of the potential risks of increased weediness of sugarcane as a result of spread and persistence of volunteer sugarcane. As sugarcane is propagated vegetatively from pieces of stalk and the seed plays no part in the production cycle, the fate of seed in the environment is yet to be studied. In this study, sugarcane seed samples, collected in fields over a 2-year period, were used to determine the overall level of sugarcane fertility, seed dormancy, and longevity of seed under field conditions. A survey of the soil seed bank in and around sugarcane fields was used to quantify the presence of sugarcane seeds and to identify and quantify the weeds that would compete with sugarcane seedlings. We demonstrated that under field conditions, sugarcane has low fertility and produces non-dormant seed. The viability of the seeds decayed rapidly (half-life between 1.5 and 2.1 months). This means that, in Australia, sugarcane seeds die before they encounter climatic conditions that could allow them to germinate and establish. Finally, the soil seed bank analysis revealed that there were very few sugarcane seeds relative to the large number of weed seeds that exert a large competitive effect. In conclusion, low fertility, short persistence, and poor ability to compete limit the capacity of sugarcane seed spread and persistence in the environment.

Improvement of the CTC model seed harvester

The study aims to reduce mechanical damage to the buds and billets of seedcane, evaluating the effect of the synchronization between the speed of roller feed train and of the harvester in terms of harvested seedcane quality. A conventional harvester was improved through the introduction of hydraulic oil flow valves, who controlled the speed of the rollers using data gathered by 5 speed sensors and a data acquisition system. The trials were carried out with seeds of the CTC 9 and CTC 14 varieties under treatments of conventional and synchronized harvesting. For the CTC 9 variety the amount of inviable buds decreased from 15.8% to 12.7%. In case of the CTC 14 variety the amount of inviable buds decreased from 10.4% to 8.3%. The amount of optimum and regular billets increased by 8.2% from 65.9 % to 74.1%. In general, the changes proposed, linked to the synchronism of speed, have shown promise in increasing the quality of sugarcane seeds obtained by mechanical harvesting.

Abiotic Limits for Germination of Sugarcane Seed in Relation to Environmental Spread.

Sugarcane is a vegetatively propagated crop and hence the production of seed and its fate in the environment has not been studied. The recent development of genetically modified sugarcane, with the aim of commercial production, requires a research effort to understand sugarcane reproductive biology. This study contributes to this understanding by defining the abiotic limits for sugarcane seed germination. Using seed from multiple genetic crosses, germination was measured under different light regimes (light and dark), temperatures (from 18 °C to 42 °C) and water potentials (from 0 MPa to −1 MPa); cardinal temperatures and base water potential of germination were estimated based on the rates of germination. We found that sugarcane seed could germinate over a broad range of temperatures (from 11 °C to 42 °C) with optima ranging from 27 °C to 36 °C depending on source of seed. Water potentials below −0.5 MPa halved the proportion of seed that germinated. By comparing these limits to the environmental conditions in areas where sugarcane grows and has the potential to produce seed, water, but not temperature, will be the main limiting factor for germination. This new information can be taken into account when evaluating any risk of weediness during the assessment of GM sugarcane.

Effect of substrates and plant growth promoting bacteria in the germination of sugarcane seeds.

The aim of this work was to test different substrates with Plant Growth Promoting Bacteria (PGPB) inoculation on sugarcane seed germination. The substrates were sand, vermiculite and Plantmax®. The completely randomized factorial design 2x3, with 4 repetitions was used. The parameters estimated were speed of germination index, days for emergence, and 30 days after sowing the parameters: height of seedlings (cm), volume of roots (cm3), length of roots (cm), and the number of germinated plants. The application of PGPB promoted better development of seedlings, mainly roots. The Plantmax® presented the better conditions for germination and seedling development. Vermiculite had the worst results. No response to PGPB was observed in the sand. The use of Plantmax® and PGPB in germination of sugarcane seeds is recommended.

Agrobacterium tumefaciens-mediated in planta seed transformation strategy in sugarcane

An efficient, reproducible and genotype-independent in planta transformation has been standardized for sugarcane using seed as explant. Transgenic sugarcane production through Agrobacterium infection followed by in vitro regeneration is a time-consuming process and highly genotype dependent. To obtain more number of transformed sugarcane plants in a relatively short duration, sugarcane seeds were infected with Agrobacterium tumefaciens EHA 105 harboring pCAMBIA 1304-bar and transformed plants were successfully established without undergoing in vitro regeneration. Various factors affecting sugarcane seed transformation were optimized, including pre-culture duration, acetosyringone concentration, surfactants, co-cultivation, sonication and vacuum infiltration duration. The transformed sugarcane plants were selected against BASTA(®) and screened by GUS and GFP visual assay, PCR and Southern hybridization. Among the different combinations and concentrations tested, when 12-h pre-cultured seeds were sonicated for 10min and 3min vacuum infiltered in 100 µM acetosyringone and 0.1% Silwett L-77 containing Agrobacterium suspension and co-cultivated for 72-h showed highest transformation efficiency. The amenability of the standardized protocol was tested on five genotypes. It was found that all the tested genotypes responded favorably, though CoC671 proved to be the best responding cultivar with 45.4% transformation efficiency. The developed protocol is cost-effective, efficient and genotype independent without involvement of any tissue culture procedure and can generate a relatively large number of transgenic plants in approximately 2months.

Optimal Design for Cutting Mechanism of the Sugarcane Planting

In this paper, mainly according to the selection of sugar cane seeds and the quality requirements for cutting, we design a cutting mechanism not only to cut off the sugar cane , but also can ensure the cutting sugarcane planting to achieve the agronomic requirements for sugar cane . The institution's overall ideas are as follows: first,measure different kinds of sugar cane planting in different cutting force and cutting speed in case of broken head.After preliminary analysis,gain cutting force,cutting speed ,the diameter of sugar cane ,the species of sugar cane ,cutting blade edge angle, cutting angle and cutting mode correlation.Then select the power transfer device and the working parts of the disk and blade according to the obtained data.Last design drive of belt wheel, drive shaft, bearing type, bearing cover through calculation and checking.The successful design and development of the institution of sugar cane cutting mechanism will be able to greatly reduce the labor intensity and production costs in the process of sugarcane planting.

Fertilidade de cruzamentos, potencial fisiológico e armazenamento de sementes de cana-de-açúcar

Sugar cane (Saccharum sp.) is one of the main agricultural products in Brazil. For its cultivation it is used stems or rhizomes, which enable to reproduce the same genotype infinite times. However, in breeding programs, the sexual reproduction is necessary to produce new genetic combinations, and the use of seeds is the basis for obtaining more productive varieties. Thus, this study aimed to assess the fertility of bi-parental and multiple crossings, involving varieties RB92579 and RB92606, as well as to determine the physiological potential and storage of seeds (spikelets) produced. The determination of the fertility of crossings was performed by evaluating the percentage of caryopses produced in each crossing and the seed physiological potential was determined by the use of standard germination and vigor. To assess the potential of storage, seeds were kept during six months in kraft paper bags under cold room at 21 ± 4.5 ° C and relative humidity of 44± 14%, and the assessments conducted monthly by germination and vigor tests. The fertility and physiological potential of sugar cane seeds are strongly affected by the type of crossing involving varieties RB92579 and RB92606. Seed performance, despite minor fluctuations over time, was kept for six months of storage.

Fertility of crossings, physiological potential and seed storage of sugar cane

Sugar cane (Saccharum sp.) is one of the main agricultural products in Brazil. For its cultivation it is used stems or rhizomes, which enable to reproduce the same genotype infinite times. However, in breeding programs, the sexual reproduction is necessary to produce new genetic combinations, and the use of seeds is the basis for obtaining more productive varieties. Thus, this study aimed to assess the fertility of bi-parental and multiple crossings, involving varieties RB92579 and RB92606, as well as to determine the physiological potential and storage of seeds (spikelets) produced. The determination of the fertility of crossings was performed by evaluating the percentage of caryopses produced in each crossing and the seed physiological potential was determined by the use of standard germination and vigor. To assess the potential of storage, seeds were kept during six months in kraft paper bags under cold room at 21 ± 4.5 ° C and relative humidity of 44± 14%, and the assessments conducted monthly by germination and vigor tests. The fertility and physiological potential of sugar cane seeds are strongly affected by the type of crossing involving varieties RB92579 and RB92606. Seed performance, despite minor fluctuations over time, was kept for six months of storage.