Lactic acid has applications in many different sectors such as food, chemicals and polymers. Its production from lignocellulosic materials has been highlighted as a possibility to overcome barriers in the production chain such as decreasing process costs and reaching better environmental performance, besides adding value to biomass. However, the needed pre-treatment that lignocellulosic material goes through to release fermentable sugars also produces inhibitor compounds for the microorganisms, such as HMF and furfural. In this work, a Bacillus coagulans strain is evaluated as an l-lactic acid producer using sugarcane bagasse hemicellulosic hydrolysate. l-Lactic acid production reached 55.99 g/L, 0.87 g/g of yield, 1.7 g/ L h −1 of productivity and 99.4% of isomeric purity. Simultaneous bio-detoxification removed 100% of HMF and furfural without any treatment prior. To the best of our knowledge, it is the first time that B. coagulans is used to lactic acid production and bio-detoxification tests.