Sugar Analysis Research Articles

Relationships of Light to Enzyme and Sucrose Transformations in Sugarcane Treated with Paraquat

Relationships of Light to Enzyme and Sucrose Transformations in Sugarcane Treated with Paraquat

The K26 capsular polysaccharide from Acinetobacter baumannii KZ-1098: Structure and cleavage by a specific phage depolymerase

The KL26 gene cluster responsible for the synthesis of the K26 capsular polysaccharide (CPS) of Acinetobacter baumannii includes rmlBDAC genes for l-rhamnose (l-Rhap) synthesis, tle to generate 6-deoxy-l-talose (l-6dTalp) from l-Rhap, and a manC gene for D-mannose (D-Manp) that is rare in Acinetobacter CPS. K26 CPS material was isolated from A. baumannii isolate KZ-1098, and studied by sugar analysis, Smith degradation, and one and two-dimensional 1H and 13C NMR spectroscopy before and after O-deacetylation with aqueous ammonia. The following structure of the branched hexasaccharide repeating unit of the CPS was established:→2)−β−D−Manp−1→4−β−D−Glcp−1→3−α−L−6dTalp−1→3−β−D−GlcpNAc−(1→3↑14│Acα−L−Rhap−2←1−α−D−GlcpThe structural depolymerase of phage vB_AbaP_APK26 cleaved selectively the β-GlcpNAc-(1 → 2)-α-Manp linkage in the K26 CPS formed by WzyK26 to give monomer, dimer, and trimer of the CPS repeating unit, which were characterized by high-resolution electrospray ionization mass spectrometry as well as 1H and 13C NMR spectroscopy. The wzyK26 gene responsible for this linkage and the manC gene were only found in six A. baumannii genomes carrying KL26 and one carrying the novel KL148 gene cluster, indicating the rare occurrence of β-GlcpNAc-(1 → 2)-α-Manp in A. baumannii CPS structures. However, K26 shares a β-d-Glcp-(1 → 3)-α-l-6dTalp-(1 → 3)-β-d-GlcpNAc trisaccharide fragment with a group of related A. baumannii CPSs that have varying patterns of acetylation of l-6dTalp.

Ultraclean hybrid poplar lignins via liquid–liquid fractionation using ethanol–water solutions

As recovered from the byproducts stream of a cellulosic ethanol biorefinery, the renewable biopolymer lignin is too impure and polydisperse for many proposed applications. By mixing a hybrid poplar lignin with hot ethanol–water solutions, two liquid phases, one polymer-rich and one solvent-rich, are created. This liquid–liquid equilibrium phenomenon was used to generate solvated (and thus liquefied) lignin fractions of controlled molecular weight for which the impurities analyses for sugars and ash were near or below the limits of detection. Additionally, those carbohydrates and metals impurities end up highly concentrated in a single process stream also having potential value.

Structure and gene cluster of the O-antigen of Enterobacter cloacae G3422

The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G3422 and studied by chemical methods, including sugar analyses, Smith degradation, and solvolysis with anhydrous trifluoroacetic acid, along with 1H and 13C NMR spectroscopy. The following structure of the branched tetrasaccharide repeating unit was established: [Display omitted] The O-antigen gene cluster of Enterobacter cloacae G3422 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in a good agreement with the O-antigen structure.

Structure of the K87 capsular polysaccharide and KL87 gene cluster of Acinetobacter baumannii LUH5547 reveals a heptasaccharide repeating unit

K87 capsular polysaccharide (CPS) was isolated from Acinetobacter baumannii isolate LUH5547 that carries the KL87 capsule biosynthesis gene cluster at the chromosomal K locus. Studies by sugar analysis, selective chemical cleavages, and 1D and 2D 1H and 13C NMR spectroscopy showed that the CPS has a branched heptasaccharide repeat (K unit) containing one residue each of d-glucose (d-Glсp), d-glucuronic acid (d-GlсpA), N-acetyl-d-glucosamine (d-GlсpNAc), 6-deoxy-l-talose (l-6dTalp), and three residues of l-rhamnose (l-Rhap). The following structure of the CPS was established:→3)−α-L-Rhap−(1→2)-α-L-Rhap-(1→3)-α-L-6dTalp-(1→3)-β-D-GlcpNAc-(1→2↑1β-D-GlcpA-(4←1)-α-D-Glcp(2←1)-α-L-RhapThe position of a minor O-acetyl group present in the CPS was not determined. Functions of enzymes coded by genes in the KL87 gene cluster were tentatively assigned and found to be consistent with the CPS structure.

Structure of the 4-O-[1-Carboxyethyl]-d-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium Salinivibrio sp. EG9S8QL.

The moderately halophilic strain Salinivibrio sp. EG9S8QL was isolated among 11 halophilic strains from saline mud (Emisal Salt Company, Lake Qarun, Fayoum, Egypt). The lipopolysaccharide was extracted from dried cells of Salinivibrio sp. EG9S8QL by the phenol–water procedure. The OPS was obtained by mild acid hydrolysis of the lipopolysaccharide and was studied by sugar analysis along with 1H and 13C NMR spectroscopy, including 1H,1H COSY, TOCSY, ROESY, 1H,13C HSQC, and HMBC experiments. The OPS was found to be composed of linear tetrasaccharide repeating units of the following structure: →2)-β-Manp4Lac-(1→3)-α-ManpNAc-(1→3)-β-Rhap-(1→4)-α-GlcpNAc-(1→, where Manp4Lac is 4-O-[1-carboxyethyl]mannose.

Production of traditional milk jam with improved functional properties via probiotic and prebiotic

Objective: In this study, 3% Lactobacillus rhamnosus and Bifidobacterium animalis subsp lactis BB-12 as probiotics and 8% hazelnut paste and banana pulp as prebiotics were added to milk jam samples to produce functional milk jam. Physical, chemical, sensorial and microbiological properties of milk jam samples were discussed during 30 days of storage. Materials and Methods: The milk jam samples were stored at +4 °C for 30 days. Physicochemical (proximate analysis, acidity, hydroxymethylfurfural (HMF), total sugar, viscosity), sensorial and microbiological analysis (Lactobacillus rhamnosus and Bifidobacterium animalis subsp lactis BB-12 counts) of milk jam samples were carried out on 1st, 15th and 30th days of storage. Results: During storage, acidity, HMF, total sugar and L. rhamnosus and B. lactis BB-12 of samples were found to be 0,2-0,22%, 18,46-21,56 mg/L, 28,18 g/100 mL, 7,44-7,54 (log CFU/g) and 5,4-6,65 (log CFU/g) for control samples; 0,16-0,2%, 18,41-25,5 mg/L, 26,61 g/100mL, 7,44-7,58 (log CFU/g) and 5,6-6,54 (log CFU/g) for hazelnut paste added samples and 0,15-0,2 %, 13,6-28,65 mg/L, 24,47 g/100 mL, 7,3-7,67 (log CFU/g) and 5-6,3 (log CFU/g) for banana pulp added samples, respectively. Hazelnut paste added probiotic milk jam samples had the highest overall acceptability score (6,01) given by panellists. Conclusion: It has been determined that probiotic microorganisms in milk jam which has high sugar content maintain probiotic effect level during 30 days storage, hazelnut added as prebiotic source contributes to viability of probiotics and ensures that products are approved and consumed.

Fibroblast Growth Factor 21 and Its Association With Oxidative Stress and Lipid Profile in Type 2 Diabetes Mellitus.

IntroductionCardiovascular diseases are the leading cause of mortality in diabetic patients. Oxidative stress and mitochondrial dysfunction lead to diabetic cardiomyopathy (DCM) characterized by impaired cardiac structure and function. Hyperglycemia causes oxidative stress, which can lead to microvascular complications, macrovascular complications, and atherosclerosis. Peripheral tissues produce fibroblast growth factor 21 (FGF-21), which has anti-inflammatory properties, increases oxidation of fatty acids, and improves insulin sensitivity. Its increased levels are found in metabolic syndrome and type 2 diabetes mellitus and may also lead to coronary heart disease. Our study sought to measure the serum FGF-21 levels and their associations with lipid profile parameters and oxidative stress in patients with type 2 diabetes mellitus.MethodologyOne-hundred fifty (150) patients of both genders with type 2 diabetes mellitus were recruited along with 150 controls. Simple random sampling was done. After taking relevant history and physical examination, we drew venous blood samples of each patient and sent them to the institutional laboratory for analysis of fasting blood sugar (FBS) levels, glycated hemoglobin (HbA1C), lipid profile, and FGF-21 serum levels. Oxidative stress parameter malondialdehyde (MDA) was estimated and the total antioxidant status by ferric reducing antioxidant power assay (FRAP) was assessed. Patients were followed up after three months to record the glycemic index, and the values were recorded. We used SPSS Software 25.0 (SPSS, Inc., Chicago, USA) to analyze the data. For consideration of results to be statistically significant, a 𝑃 value of < 0.05 was selected.ResultsThe levels of serum cholesterol, triglycerides, and low-density lipoprotein (LDL) cholesterol were increased in diabetics compared to controls and were statistically significant (p<0.05). High-density lipoprotein (HDL) cholesterol was lower in diabetic patients as compared to the controls (p<0.05). There was a statistically significant increase in the level of MDA in diabetics compared to controls (p˂0.005). Serum levels of total antioxidant status (FRAP) were decreased in diabetics in comparison with controls (p˂0.005). Serum FGF-21 level was statistically increased in diabetics compared to controls (p˂0.005). FGF-21 and MDA are positively correlated and FGF-21 and FRAP are negatively correlated. Serum FGF-21 is positively correlated with total cholesterol, triglycerides, serum LDL cholesterol, and HDL cholesterol.ConclusionOur study concludes that there is a significant correlation between fibroblast growth factor 21, oxidative stress, and abnormal lipid profile in type 2 diabetic patients. FGF-21 could be the target of certain medications used to treat metabolic disorders and obesity.

Determination of Some Agronomic and Fruit Quality Characteristics of Some Watermelon Accessions from Turkish Watermelon Germplasm

This study has been conducted to determine the fruit quality parameters such as sugar and carotenoid content as well as plant and fruit characteristics of 11 local watermelon genotypes from watermelon genetic resources collection of Cukurova University, Department of Horticulture. First and 50% male and female flowering period, main stem length, main stem diameter, number of nodes on main stem, total yield, fruit weight, fruit length, fruit diameter, fruit rind thickness, total soluble solids (TSS), sugar and carotenoid contents were examined. Although there is no significant difference for main stem diameter and number of nodes on the main stem, significant differences were obtained for total yield, sugar and carotenoid composition of the genotypes. Carotenoid and sugar analysis were performed with a high-performance liquid chromatographic method coupled with diode-array detector (HPLC-DAD) and HPLC coupled with refractive index detector (RID), respectively. In all studied genotypes, cis-13-lycopene and β-carotene were the most abundant compounds. As expected for watermelon genotypes, the main sugar found in all studied genotypes was fructose. According to PCA analyses, genotypes were characterized by physical and chemical composition. Overall evaluation of results revealed that Kar 147 had better potential with carotenoid, sugar contents and fruit characteristics.

Evidence of intermolecular associations of β-glucan and high-molar mass xylan in a hot water extract of raw oat groat

High molar mass (HM) β-glucan present in oat products can reduce the risk of diet-related diseases, mainly owing to significantly increased digesta viscosity in human small intestine. To verify a research hypothesis that arabinoxylan (AX) present in oat water extract is associated with HM-β-glucan and thus may influence its functionality, multi-detection HPSEC coupled with enzymatic hydrolysis, sugar and 1H NMR analyses were performed. Isolated cell wall polysaccharide fraction comprised branched AX (arabinose-to-xylose ratio, Ara/Xyl ~ 0.8) and arabinogalactan-protein (AG-P). Nevertheless, it contained (10%) unbranched HM-xylan subfraction (weight-average molar mass, Mw ~ 3153 kg/mol), which was aggregated with β-glucan (Mw ~ 1029–1589 kg/mol) through its HM cellulose-like region. Of the two low molar mass-AXs (LM-AXs) isolated, the first having C(O)-3-monosubstituted and C(O)-2,3-disubstituted β-d-xylopyranosyl residues was covalently interlinked to HM-xylan. The second highly feruloylated, containing C(O)-3- and C(O)-2-monosubstituted units, was tightly bound to AG-P with terminal and 5-linked α-L-arabinofuranosyl residues, and non-covalently associated with LM-β-glucan subfraction.

Structure and genetics of the O-antigen of Enterobacter cloacae K7 containing di-N-acetylpseudaminic acid

The O-antigen (O-polysaccharide) is an essential component of lipopolysaccharide on the surface of Gram-negative bacteria and plays an important role in interaction with host organisms. In this study, we investigated the chemical structure and characterized the gene cluster of Enterobacter cloacae K7 O-antigen. As judged by sugar analyses along with NMR spectroscopy data, E. cloacae K7 antigen has a tetrasaccharide O-unit with the following structure:→8)-β-Psep5Ac7Ac-(2 → 2)-β-l-Rhap-(1 → 4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1→The O-antigen gene cluster of E. cloacae K7 between conserved genes galF and gnd was sequenced. Most genes necessary for the O-antigen synthesis were found in the cluster and their functions were tentatively assigned by comparison with sequences in the available databases.

Comparison of ultraviolet and refractive index detections in the HPLC analysis of sugars

Refractive index (RI) detection is the standard approach for quantitatively detecting sugars via high-performance liquid chromatography (HPLC), while ultraviolet (UV) absorbance detection is the most commonly used detection method for general HPLC analysis. We compared the two detection approaches of UV and RI in the HPLC analysis of small sugars to investigate whether UV detection could be an alternative method to RI detection. UV detection was performed using a photodiode array scanning from 190 to 400 nm. We obtained comparable limit of detection (LOD) results for RI and UV detection in the HPLC analysis of monosaccharides, while HPLC-RI provided better LOD results than HPLC-UV in disaccharide analysis. Both HPLC-RI and HPLC-UV methods were applied to analyze a real honey sample, and similar results were obtained in terms of precision and recovery. The study conclusively shows that the UV-based HPLC analysis of sugars offers a sufficient alternative to RI-based HPLC analysis.

The Effect of the Cultivar and Harvest Term on the Yield and Nutritional Value of Rhubarb Juice

Since scientific interest in rhubarb from a culinary point of view is a relatively new issue, the aim of this study was to test five edible cultivars of Rheum rhabarbarum L. (‘Poncho’, ‘Canadian Red’, ‘Valentine’, ‘Red Champagne’, and ‘Victoria’) from a specific culinary perspective, i.e., processing into juice. Total yields (t/ha) were established in six harvests during a two-year field experiment. For juice production and subsequent laboratory analysis, rhubarb petioles from two different harvest terms were used (i.e., harvest term A (HTA) and harvest term B (HTB)). Analyses of total sugar, glucose, fructose, total soluble solids (TSS), total acidity, malic acid, and pH level were determined by FT-IR spectrophotometer. Total yields of petioles varied between 28.77 t/ha (‘Canadian Red’) and 45.58 t/ha (‘Red Champagne’) at a density of 11,000 pl/ha. ‘Red Champagne’ significantly (p < 0.05) reached the highest juice yield potential (85%) and the highest values of glucose (9.97 g/L), total soluble solids (4.37 g/L), and total sugars (54.96 g/L).

Three β-Glucuronosyltransferase Genes Involved in Arabinogalactan Biosynthesis Function in Arabidopsis Growth and Development.

Arabinogalactan proteins (AGPs) contain arabinogalactan (AG) polysaccharides that are biologically relevant to plant growth processes. Here, the biochemical and physiological roles of three Golgi localized β-glucuronosyltransferase genes (GLCAT14A, GLCAT14B and GLCAT14C) in Arabidopsis thaliana, responsible for the addition of glucuronic acid to AG chains, were further investigated using single, double and triple glcat14 mutant plants. These proteins were localized to the Golgi apparatus when transiently expressed in Nicotiana benthamiana. Sugar analysis of AGP extracts from Arabidopsis stem, leaf and siliques showed a consistent reduction in glucuronic acid in glcat14 mutants relative to wild type, with concomitant effects resulting in tissue-specific alterations, especially in arabinose and galactose sugars. Although we observed defects in trichome branching in glca14a/b and glca14a/b/c mutants, scanning electron microscope analysis/energy dispersive microanalysis (SEM/EDX) showed no difference in the calcium content of trichomes in these mutants relative to wild type. Immunoblot analyses of the stem and leaf showed a reduction in AGPs as detected with the LM2 antibody in glcat14a/b and glcat14a/b/c mutants relative to wild type. The current work exemplifies the possibility of conducting structure-function assessment of cell wall biosynthetic genes to identify their physiological roles in plant growth and development.

Enhancing the Efficiency of Soybean Inoculant for Nodulation under Multi-Environmental Stress Conditions.

The development of rhizobial inoculants with increased resistance to abiotic stress is critical to mitigating the challenges related to climate change. This study aims at developing a soybean stress-tolerant Bradyrhizobium inoculant to be used under the mixed stress conditions of acidity, high temperature, and drought. Six isolates of Bradyrhizobium with high symbiotic performance on soybean were tested to determine their growth or survival abilities under in vitro conditions. The representative stress-tolerant Bradyrhizobium isolates 184, 188, and 194 were selected to test their ability to promote soybean growth under stress conditions compared to the type strain Bradyrhizobium diazoefficiens USDA110. The plant experiment indicated that isolate 194 performed better in symbiosis with soybean than other Bradyrhizobium strains under stress conditions. Based on the stress tolerance index, soybeans inoculated with isolate 194 showed a high growth performance and significantly better nodulation competition ability than USDA110 under several stress conditions. Interestingly, supplementation of sucrose in the culture medium significantly enhances the survival of the isolate and leads to improved plant biomass under various stress conditions. Analysis of the intra-cellular sugars of isolate 194 supplemented with sucrose showed the accumulation of compatible solutes, such as trehalose and glycerol, that may act as osmoprotectants. This study indicates that inoculation of stress-tolerant Bradyrhizobium together with sucrose supplementation in a medium could enhance bacterial survival and symbiosis efficiency under stress conditions. Although it can be applied for inoculant production, this strategy requires validation of its performance in field conditions before adopting this technology.

Structural studies on the O-specific polysaccharide of the lipopolysaccharide from Pseudomonas donghuensis strain SVBP6, with antifungal activity against the phytopathogenic fungus Macrophomina phaseolina

An O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide (LPS) of Pseudomonas donghuensis SVBP6, a bacterium with a broad-spectrum antifungal activity in vitro, particularly that against Macrophomina phaseolina. This latter is one of the most virulent and dangerous pathogens of plants, including soybean which is an economically important crop in Argentina today. The OPS was studied by sugar analysis and spectroscopy (1D and 2D 1H and 13C NMR) showing the following trisaccharide repeating unit:→6)-ɑ-D-ManpNAc-(1 → 3)-β-l-Rhap-(1 → 4)-β-D-Glcp-(1→.The crude LPS, the purified LPS and the O-chain were assayed for their antifungal activity against M. phaseolina at 25, 50, 100, and 200 μg plug−1. The results showed that the crude LPS best inhibition was at 200 μg plug−1, able to inhibit the fungus growth by about 45%, while purified LPS and the corresponding OPS, in the same condition, reduced fungus growth by 65%, and 75%, respectively. Furthermore, the purified LPS and OPS significantly reduced the growth of M. phaseolina already at 100 μg plug−1 compared to the crude LPS. The structure of the O-chain is unique among the bacterial LPS and this is the first time that both the antifungal activity of a bacterial LPS and its corresponding O-chain were described.

Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides.

Whole genome sequences of two Acinetobacter baumannii clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously described KL11 and KL83 gene clusters, sharing genes for the synthesis of l-rhamnose (l-Rhap) and 6-deoxy-l-talose (l-6dTalp). CPS material isolated from 48-1789 and MAR24 was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. The structures of K106 and K112 oligosaccharide repeats (K units) l-6dTalp-(1→3)-D-GlcpNAc tetrasaccharide fragment share the responsible genes in the respective gene clusters. The K106 and K83 CPSs also have the same linkage between K units. The KL112 cluster includes an additional glycosyltransferase gene, Gtr183, and the K112 unit includes α l-Rhap side chain that is not found in the K106 structure. K112 further differs in the linkage between K units formed by the Wzy polymerase, and a different wzy gene is found in KL112. However, though both KL106 and KL112 share the atr8 acetyltransferase gene with KL83, only K83 is acetylated.

Comparative Analysis of Sugar and Organic Acid Contents of Different Apple Cultivars in Dryland of Loess Plateau

In order to clarify the characteristics of soluble sugar and organic acid contents among different varieties of apple fruits in the dry area of loess plateau, the contents of sugar and acid in the fruits of 30 main varieties were analyzed. The soluble sugar and organic acid contents in 30 varieties of ripe fruits were tested by high-performance liquid chromatography (HPLC), and the data were analyzed for one-way ANOVA and correlation. The results showed that the fructose contents was the highest in different varieties of apple fruit sugar group with an average value of 52.964 mg/g, accounting for 48.52% of the total sugar contents, and the two were extremely significantly related (r=0.787), and the highest fructose contents was ‘gala’ (69.736 mg/g), the sucrose contents in most apple varieties were higher than glucose, and only 3 varieties showed that glucose contents were higher than sucrose contents, respectively ‘Golden Delicious’, ‘August Fuji’ and ‘Gold Spur’. The main organic acid in different apple varieties was malic acid, with an average value of 3.857 mg/g, accounting for 58.74% of the total acid contents, and the two were extremely significantly related (r=0.845), the contents of quinine acid was second, other types of organic acids contents were low, the variety with the highest malic acid contents was ‘Hanfu’ (8.109 mg/g). There were 3 varieties with sweetness value/total acid contents greater than 30, respectively ‘World No1’, ‘Golden Delicious’ and ‘Gala’, and 13 varieties between 20 and 30, and 14 varieties with less than 20. The sweetness value and total acid contents of apple fruit, as well as their ratio affected the flavor of fruit together.

Investigation methods for detecting honey samples adulterated with sucrose syrup

Various adulterations and thermal treatments applied to honey during or after production interfere with the natural structure of honey and reduce its nutritional value. In this study, three different honey samples were adulterated with sucrose (sugar beet) syrup at the ratios of 0, 10, 20, 40, and 50 % in weight to prepare adulterated honey samples. HPLC was used for the sugar analysis while an atomic absorption (AA) spectrophotometer was used for the potassium and sodium analyses, and other chemical and physical analyses were conducted on these adulterated honey samples. The PCA analysis results showed that the parameters analysed in this study can also be used in the analysis of adulterated honey. As a result, it was determined that some components of the samples that had been adulterated by the addition of sucrose at ratios as high as 50 % may go out of the known variation ranges; in other words, they push the limits. An increase was found in the 5-hydroxymethylfurfural (HMF) amounts in the thermally treated samples while a reduction was observed in diastase activity. These results may be considered an indicator of the fact that honey has been heated.

Chemical constituents of fresh-cut potato as affected by cultivar, age, storage, and cooking.

Certain changes in phenolics and sugars can occur during the storage of potatoes, where particularly amounts of sugars represent the critical factor as they are involved in potentially harmful acrylamide (AA) formation during frying. This research investigates the impact of cultivars (Birgit and Lady Claire), tuber's age (1, 5, and 9 months), and storage duration (1, 5, and 8 days at 10 °C) on the content of phenolics and sugars in raw, boiled, and fried fresh-cut potato (FCP). The influence of these factors on the formation of AA in fried FCP was also assessed. Significant differences in phenolics and sugars were observed between cultivars (cv. Birgit contained 5.77mg of phenolics 100g-1 of dry weight (DW) and 1.75g of sugars 100g-1 DW, while cv. Lady Claire contained 10.13mg of phenolics 100g-1 DW and 0.65g of sugars 100g-1 DW). The content of phenolics significantly decreased, while sugars increased during tubers' aging. FCP storage time had no significant influence on the content of phenolics and sugars. The phenolics and sugars were the highest in the raw samples and the lowest in the boiled ones. Although the AA level in fried samples was significantly influenced by cultivar (Birgit>Lady Claire) and it increased with FCP storage time, it was below European Food Safety Authority (EFSA) regulation's approved maximum value (750µg kg-1 FW) in both cultivars. Therefore, cvs. Birgit and Lady Claire could be considered as promising FCP cultivars and for frying purposes. PRACTICAL APPLICATION: The results of this research reveal that quantitative changes of chemical constituents occurring during storage and cooking of fresh-cut slices of potato cultivars Birgit and Lady Claire are not a concern. This is of particular importance to fresh-cut producers and customers. Phenolics were reduced during storage, but they were still present in all cooked samples independently of the cooking method. The analysis of sugars showed that tubers as old as 9 months could be used for fresh-cut potato processing, providing safe frying without critical levels of acrylamide.