Sufficient Supply Research Articles

Allelopathic interactions of brown mustard (Brassica juncea L.) with other aromatic plants during germination and seedling development

Organic compounds originating from plant metabolism and reaching the environment may inhibit or stimulate germination and early developmental processes of other plants grown in the vicinity. The aim of this study was to investigate the allelopathic interactions of brown mustard with four other aromatic plants (summer savoury, coriander, caraway and common sage). While health-promoting metabolites of brown mustard are thoroughly investigated and the plant receives an increasing interest from cultivators of aromatic and medicinal plants, its allelopathic interactions with other aromatic plants are poorly documented. The experiments were performed in closed germination vessels, under controlled conditions. The influence of the presence or absence of light, as well as of a sufficient or reduced water supply on the co-germination of brown mustard seeds with each of the other four aromatic plants was also evaluated. The presence of brown mustard reduced the germination velocity of caraway, coriander, sage and savoury. It inhibited stem elongation, but stimulated root growth of savoury. Shoot growth of coriander seedlings was enhanced by the vicinity of brown mustard plantlets. Caraway slowed down the germination of brown mustard seeds, but it stimulated root and stem elongation of the seedlings. The presence of savoury enhanced the fresh biomass production of brown mustard. Common sage inhibited root growth, but stimulated stem elongation of brown mustard plantlets. Most of the allelopathic effects were more pronounced under water shortage than under sufficient water supply. Positive interspecific influences on biomass production were, in most cases, more pronounced in the presence of light. The findings may contribute to optimization of brown mustard cultivation in small parcels, in the vicinity of other aromatic plants, some allelopathic interactions being able to stimulate growth and developmental processes in an environmental-friendly and cost-effective manner.

Factors Impacting Compliance with Infection Control Guidelines among healthcare providers in Neonatal Intensive Care Unit, referral hospital, Saudi Arabia

Healthcare-associated infections continue to be a major problem that endangers patient safety, especially for critically ill hospitalized patients. Reducing the risk of this infection can be achieved through improving compliance with infection control guidelines and identifying and controlling the associated factors. This study aim to investigate factors impacting compliance with infection control guidelines and increasing the risk of healthcare-associated infections in neonatal intensive care units. A qualitative approach, a one-to-one interview design, was used, between April 15 and May 15, 2021, to interview ten participants who worked in the neonatal intensive care unit at a Saudi Arabian referral hospital. Through data analysis, two major themes and four sub-themes were found, which include a factor related to the workforce (shortage of staff and lack of knowledge) and factors related to the workplace (shortage of supplies and lack of monitoring). In conclusion, healthcare-associated infections in neonatal intensive care units are a serious issue that could lead to an increase in mortality and morbidity rates among patients. Thus, healthcare settings' commitment to improve compliance with the infection control guidelines is essential to lowering the incidence of healthcare-associated infections. This can be achieved through proper staffing, sufficient supplies, and effective monitoring.

Blood Donor Incentives across 63 Countries: The BEST Collaborative Study

Incentives for blood donors are a much-debated strategy intended to ensure a sufficient supply of blood. Yet, there is a fundamental lack of knowledge about which incentives are offered by different blood collectors. We provide a comprehensive description of incentive policies for whole blood donors across 63 countries and 50 states of the United States. We collected data on incentive policies by conducting 2 surveys among representatives of blood collection establishments. Additionally, we integrated incentive data from an existing study and the World Health Organization (WHO). Lastly, we performed a web content analysis of blood collector websites and news releases to extend incentive data for the United States as well as underrepresented regions. We present descriptive analyses illustrating the type and value of incentives and their geographical distribution around the globe. Approximately half of the countries in our sample employ financial incentives, which include cash and tax benefits, but also less conventional incentives, such as healthcare supplements and raffles. Time off work is also commonly offered to blood donors and varies across blood collection establishments in duration and whether it is granted to all donors or only to those whose employer allows it. There is a geographical clustering of incentives, such that neighboring countries are more likely to employ similar incentives. This study provides insights into the strategies used for incentivizing blood donation and highlights the global diversity of incentive policies for whole blood donors. In stark contrast to WHO guidelines, half of the countries surveyed employ some kind of high-value incentive for blood donors. More realistic guidelines that are adapted to the local cultural and institutional context may be needed to maintain an adequate blood supply.

Power load analysis and configuration optimization of solar thermal-PV hybrid microgrid based on building

Solar thermal power system and photovoltaic coupled system can supply electric energy based on renewable solar energy. To explore the optimal configuration of hybrid microgrid driven by solar energy and to achieve a stable and sufficient electric power supply for the distributed energy system, this paper configures a solar thermal-photovoltaic hybrid microgrid and compares the performance of several microgrids based on the power load analysis of the building. The working fluid and the microgrid configuration are determined based on the performance evaluation of single and hybrid microgrids. The effect of solar irradiation on economic and environmental performance is analyzed under the meteorological data of Xi'an and Lhasa in China. Furthermore, the configuration optimizations of a hybrid microgrid based on the actual load and basic load of the building are carried out to examine the performance of the system. Results indicate that economic and environmental performances are sensitive to solar irradiation. More than 50 % of the area of solar thermal collector can be saved for Lhasa compared with Xi'an. Solar thermal-photovoltaic hybrid microgrid with battery can decrease carbon emission and avoid dependence on the external power grid.

Co-precipitation of iron and silicon: Reaction kinetics, elemental ratios and the influence of phosphorus

A sufficient supply of dissolved silicon (DSi) relative to dissolved phosphorus (DP) may decrease the likelihood of harmful algal blooms in eutrophic waters. Oxidative precipitation of Fe(II) at oxic-anoxic interfaces may contribute to the immobilization of DSi, thereby exerting control over the DSi availability in the overlying water. Nevertheless, the efficacy of DSi immobilization in this context remains to be precisely determined. To investigate the behavior of DSi during Fe(II) oxidation, anoxic solutions containing mixtures of aqueous Fe(II), DSi, and dissolved phosphorus (DP) were exposed to dissolved oxygen (DO) in the batch system. The experimental data, combined with kinetic reaction modeling, indicate that DSi removal during Fe(II) oxidation occurs via two pathways. At the beginning of the experiments, the oxidation of Fe(II)-DSi complexes induces the fast removal of DSi. Upon complete oxidation of Fe(II), further DSi removal is due to adsorption to surface sites of the Fe(III) oxyhydroxides. The presence of DP effectively competes with DSi via both of these pathways during the initial and later stages of the experiments, with as a result more limited removal of DSi during Fe(II) oxidation. Overall, we conclude that at near neutral pH the oxidation of Fe(II) has considerable capacity to immobilize DSi, where the rapid homogeneous oxidation of Fe(II)-DSi results in greater DSi removal compared to surface adsorption. Elevated DP concentration, however, effectively outcompetes DSi in co-precipitation interactions, potentially contributing to enhanced DSi availability within aquatic systems.

Effects of oxygen enrichment on diesel spray flame soot formation in O2/Ar atmosphere

In this study, diesel spray combustion at oxygen-enriched conditions (oxygen volume fraction of 21–70 %) with argon dilution is experimentally investigated in a constant-volume combustion chamber. Optical diagnostics are employed to study flame development, stabilization, and soot formation at oxygen-enriched conditions. To further verify the experimental observations, two-stage Lagrangian simulations are used to analyze the effects of oxygen on the formation and oxidation of soot precursors, polycyclic aromatic hydrocarbons. Results show that replacing nitrogen in air by argon leads to a 50 % reduction of the flame lift-off length, an increased soot flame temperature by 300 K, and higher soot concentrations. Flame morphology and structure still follow the classic conventional diesel combustion model in the oxygen range of 21–40 %, while changes are observed when oxygen levels are higher than 50 %. The width and length of the soot flame are shortened, and chemiluminescence from intermediate species like CO dominates the flame natural luminosity at the spray head, where the flame temperature reaches near 3000 K. Soot reduction mechanisms at high-degree oxygen-enrichment conditions are investigated. The intrinsic mixing-limited combustion of diesel sprays leads to unavoidable fuel-rich areas locally, but the shortened flame lift-off length and sufficient oxygen supply confines soot-forming conditions to a smaller, upstream region. The residence time of fuel parcels in this confined soot-forming area is shortened due to the larger local spray velocity. Thereafter, fuel parcels enter a high-temperature fuel-lean region, where the formed soot is oxidized rapidly.

COVID-19 vaccination strategies in settings with limited rollout capacity: a mathematical modelling case study in Sierra Leone

BackgroundCOVID-19 vaccine coverage in low- and middle-income countries continues to be challenging. As supplies increase, coverage is increasingly becoming determined by rollout capacity.MethodsWe developed a deterministic compartmental model of COVID-19 transmission to explore how age-, risk-, and dose-specific vaccine prioritisation strategies can minimise severe outcomes of COVID-19 in Sierra Leone.ResultsPrioritising booster doses to older adults and adults with comorbidities could reduce the incidence of severe disease by 23% and deaths by 34% compared to the use of these doses as primary doses for all adults. Providing a booster dose to pregnant women who present to antenatal care could prevent 38% of neonatal deaths associated with COVID-19 infection during pregnancy. The vaccination of children is not justified unless there is sufficient supply to not affect doses delivered to adults.ConclusionsOur paper supports current WHO SAGE vaccine prioritisation guidelines (released January 2022). Individuals who are at the highest risk of developing severe outcomes should be prioritised, and opportunistic vaccination strategies considered in settings with limited rollout capacity.

Virginia data reveals severe shortage of child, adolescent psychiatrists

There is a significant shortage of practicing child and adolescent psychiatrists, or CAPs, across the United States, according to data from the American Academy of Child and Adolescent Psychiatry (AACAP), the online news agency, Virginia Mercury, reported Dec. 4. The AACAP data classified states into four groups: states with a sufficient supply of CAPs, those with a high shortage, those with a a severe shortage, and those states with no CAPs. Virginia is in a severe shortage, with just 264 CAPs in the state as of 2019, the most recent data available. That means on average there are 14 CAPs available per 100,000 children in Virginia, which is also the national average. The academy defines a “mostly sufficient supply” as more than 47 per 100,000 children. Nationwide, few counties meet that standard. The Virginia Mental Health Access Program, or VMAP, and oversees the work of all child psychiatrists involved in the program. VMAP is a statewide initiative that strives to give kids greater mental health access by teaching pediatricians to function like psychiatrists. Pediatricians are taught how to screen, diagnose, manage and treat mental health in children. More than $12 million was allocated for child psychiatry and children's crisis response services to be divided throughout the state based on current services already offered. The funds can be used to hire or contract child psychiatrists to provide clinical services, or to train. Mental health advocates have said the budget makes “significant investments” in mental health services across the state.

A new species of Keteleeria (Pinaceae) from the Lower Cretaceous of Inner Mongolia, Northeast China, and its palaeogeographic and palaeoclimatic implications

Keteleeria Carrière (Pinaceae) is a small genus of evergreen conifer trees, with three to five extant species and six variants distributed across China, Laos, and Vietnam. A new species of conifer fossil wood, Keteleeria huolinhensis sp. nov., is described from the Lower Cretaceous Huolinhe Formation in Inner Mongolia, China. This species is characterized by a heterogeneous pith, endarch primary xylem, the presence of axial resin canals, abietinean radial tracheid pitting, mostly taxodioid and occasionally cupressoid cross-field pitting, nodular horizontal and end walls of ray parenchyma cells, and uniseriate rays of 1–15 (mainly 1–8) cell height. This newly discovered fossil wood represents the earliest record of Keteleeria wood, and sheds light on its evolutionary history and palaeogeographical distribution ranges in the geological past. Cladistic analysis based on 12 morphological characteristics supports the assignment of Protopiceoxylon as the ancestral group of Keteleerioxylon and Keteleeria, reflecting the evolution of radial tracheid pitting from the mixed to abietinean type. Quantitative analysis of the growth rings indicated that K. huolinhensis sp. nov. is an evergreen tree with a Leaf Retention Time (LRT) of 1–3 years. The growth ring patterns in the present fossil wood specimen suggest that the Huolinhe Basin experienced a temperate climate with regular seasonal fluctuations, and relatively sufficient water supply during the Early Cretaceous. Traumatic resin canals, wound scars, presumed fungal remains, and insect tracks in the stem contribute to a further understanding of the complex ecological relationships in the Early Cretaceous Huolinhe flora.

A Study of the Influence of Environmental Factors on Water–Heat Exchange Process in Alpine Wetlands

Wetlands, which are composed of soil, vegetation and water, have sufficient water supply and are sensitive to climate change. This study analyzes the coupling degree between wetlands and atmosphere and discusses the influence of environmental factors (solar radiation and water vapor pressure deficit) on latent heat flux by using the experimental data from the Maduo Observatory of Climate and Environment of the Northwest Institute of Eco-Environment and Resource, CAS and WRF models. The results showed that, during the vegetation growing season, the average value of Ω (decoupling factor) is 0.38 in alpine wetlands, indicating that the coupling between wetlands and atmosphere is poor. Solar radiation is the main factor influencing the latent heat flux in the results of both observation data analysis and model simulation, and solar radiation and water vapor pressure deficit still have the opposite reaction to latent heat flux; when solar radiation increased by 30%, the average daily amount of latent heat flux increased from 5.57 MJ·m−2 to 7.50 MJ·m−2; when water vapor pressure deficit increased by 30%, the average daily amount of latent heat flux decreased to 5.17 MJ·m−2. This study provides a new research approach for the study of the parameterization of latent heat flux and evapotranspiration in the context of global climate change

Contrasting characteristics of continental and oceanic deep convective systems at different life stages from CloudSat observations

The deep convective system (DCS) has large impacts on the radiation balance, global circulation and consequent the climate change. However, a reasonable representation of the DCS characterization in numerical models is limited by the understanding of its evolutionary mechanism. In this study, we investigate the land-ocean DCS contrast using three years (2015–2017) of cloud profiling radar (CPR) observations from the CloudSat satellite based on a connected area DCS identification approach. We identify the DCS life stages through a buoyancy determination, and conduct a composite image method to provide robust internal statistical structure of the morphology, radar reflectivity and microphysical properties of oceanic and continental DCS during each life stage. Reasonable evolutionary mechanisms of DCSs over ocean and land is proposed. It is found that the continental DCS height is strongly influenced by the intense of convection, and its volume decrease when heavy precipitation occurs. While the oceanic DCS can maintain a larger volume due to the sufficient water vapor supply. It often presents with dispersed stratified precipitation and the main procedure of its dissipation is the cloud edge entrainment. Our results may provide a pathway to evaluate the life cycle and evolution mechanism of DCS from model simulation.

Drought’s Impact on Growth and Strategies to Mitigate its Effects on Potato Cultivation: A review

The potato is the third most widely produced cereal in the world, behind rice and wheat. The contemporary potato is vulnerable to yield loss in prolonged droughts. Droughts are becoming more severe and common as a result of global warming. Because of its shallow root system, potatoes are easily parched. The occurrence of drought and water shortage has substantial ramifications for the production of potatoes, exerting influence on both the volume and quality of potato harvests. Potatoes are a crop that needs a substantial amount of water, since they rely on a regular and sufficient water supply during their whole growth cycle. The presence of drought conditions or limited water supplies might result in many adverse consequences for the cultivation of potatoes. Potato plants are susceptible to experiencing stunted growth and development as a direct result of the water stress that is brought on by drought conditions. The restricted supply of water adversely affects the absorption of vital nutrients by plants, resulting in imbalances and deficits in nutritional levels. These conditions may lead to inhibited development, diminished leaf surface, and a lower quantity of tubers. Water stress circumstances have a considerable negative impact on the total production potential of potato crops. Moreover, the phenomenon of water shortage has a significant impact on the physiological mechanisms of potato plants, specifically in relation to essential functions like photosynthesis and transpiration. A scarcity of water during the tuber bulking phases might lead to a reduction in potato size, hence impacting its commercial worth. The use of effective irrigation systems, such as drip irrigation or precision sprinklers, has the potential to optimize water usage and minimize losses resulting from evaporation. The use of water conservation methods and the adoption of drought-resistant potato cultivars are crucial solutions for mitigating the effects of water shortage on potato farming. Through the use of these procedures, farmers have the ability to augment the resilience of their potato crops and guarantee the attainment of sustainable production, even when faced with conditions of low water availability.

The alternative policies to sustain the level of sufficient domestic rice staple food supply for the population (A case study in Banyumas Regency, Central Java, Indonesia)

The Indonesian government has implemented various policies to meet the population’s need to raise staple food. Global food crises and malnutrition issues arise from several factors including population growth, uncontrolled conversion of rice fields, shifting consumption patterns, and climate change. Therefore, efforts to maintain and increase domestic rice production have become crucial to reduce potential food vulnerabilities and dependence on other countries for food. This study aims to analyze the dynamics of domestic rice sufficiency in the Banyumas Regency, analyze key related variables, and formulate policies to maintain it. The research methods used were visual image interpretation, building dynamic models, and conducting prospective analysis using the MICMAC method. The results show that domestic rice sufficiency is decreasing, resulting in a widening gap between the population’s rice needs and the domestic rice supply. The domestic rice supply in the study area can meet the rice needs of the local population by 2025, and in 2026, a deficit will begin to occur. Policies that can be implemented include increasing the planting index, improving rice productivity, food diversification, and implementing regulations to protect the rice fields. Critical variables in its implementation include the Regent’s Regulation on Rice Field Protection, detailed spatial information on rice fields, irrigation water availability, and the active role of farmer groups.

Unraveling the Potential of Organic Oregano and Tarragon Essential Oils: Profiling Composition, FT-IR and Bioactivities

Oregano and tarragon are widely cultivated culinary herbs used for food seasoning, having familiar characteristic aromas appreciated by the wide public. The aim of this research was to characterize essential oils (EOs) from locally sourced organic oregano and tarragon (Cluj, Romania) and study their bioactivity potential. Results showed that oregano EO had a sesquiterpene dominant profile responsible for strong bands between 2800 and 3000 cm−1 on the Fourier transform infrared spectroscopy (FT-IR) spectrum and a composition consistent with reports from similar climatic regions. The tarragon EO profile was defined by phenylpropanoids responsible for the strong sharp peaks between 1000 and 1600 cm−1 on the FT-IR spectrum. In oregano EO, 22 compounds were identified with β-caryophyllene as a major constituent. In tarragon EO, 20 compounds were identified with eugenol as a major constituent. Oregano EO had a stronger antibacterial effect against both Gram-negative and Gram-positive bacterial strains, while tarragon EO had a slightly stronger cytotoxic effect on three types of cancer cell lines tested (skin melanoma, prostate carcinoma, and colorectal adenocarcinoma). It was concluded that, given the fact that a sufficient supply of high-quality plant material can be available for EO extraction, culinary herbs can become reliable candidates for many industries without the risk of discontinued supply. Therefore, research aiming to widen their potential applications is welcome and worth pursuing.

Safety and Efficacy of a Locally Produced Novel Anti-BCMA Chimeric Antigen Receptor T-Cell (CART) (HBI0101) for the Treatment of Relapsed and Refractory Multiple Myeloma

BACKGROUND: Anti-B-cell maturation antigen (BCMA) chimeric antigen receptor T-cell (CART) therapy shows remarkable efficacy in patients with relapsed and/or refractory (R/R) multiple myeloma (MM), with recent phase 3 studies showing clear advantage of idecabtagene vicleucel (ide-cel) and ciltacabtagene autoleucel (cilta-cel)over standard-of-care. However, access to these commercial CART products is a major barrier, and it is estimated that only around a quarter of eligible R/R MM patients will receive CART nowadays. HBI0101 is a novel second generation optimized anti-BCMA CART with 4-1BB co-stimulatory domain, that was developed in an academic setting, at Hadassah Medical Center and Bar-Ilan University. The phase Ia study evaluating HBI0101 (NCT04720313) demonstrated a manageable safety profile and an initial high efficacy (Asherie, Haematologica 2022). Here, we present the updated results of 50 patients receiving 800x10^6 CART cells in the NCT04720313 phases 1a-b / 2. METHODS: The phase 1a study with HBI0101 which first evaluated 20 R/R MM patients with ≥3 prior lines of therapy, including a PI, IMiD and anti-CD38 antibody, revealed a dose of 800x10^6 CART cells to be safe and effective. The phase Ib and phase 2 of the study evaluated this dose infusion in 43 additional patients. Inclusion criteria were relatively permissive with minimum creatinine clearance of 20ml/min, platelet count of 30x10 9/ml, left ventricular ejection fraction of 40%, and ECOG performance status of ≤2. Lymphodepletion included fludarabine 25mg/m 2 and cyclophosphamide 250mg/m 2 on days -5 to -3 before infusion (bendamustine 90mg/m 2 and on days -4 and -3 for patients with creatinine clearance <30ml/min). Planned manufacturing time was 10 days. RESULTS: Of 51 patients who underwent lymphocyte apheresis, 50 (98%) were treated with 800x10^6 CART cells; 7 patients in the phase Ia cohort and 43 in the phases 1b / 2 cohorts. HBI0101 production success rate was 100%, with manufacturing time of 10 days for all patients. The median age was 65y (range 40-84). The median number of prior lines was 4 (range 3-13) and most patients (47/50, 94%) were triple refractory to PI, IMiD and anti-CD38 antibody. Twenty patients (40%) were penta-refractory and 10 (20%) were refractory to the anti-BCMA antibody drug conjugate belantamab mafodotin. Forty-six patients (92%) were refractory to the last line of therapy. Twelve patients (24%) had high risk cytogenetics (t(4:14), t(14:16) or deletion 17p); 31 (62%) when 1q gain included. Sixteen patients (32%) had extramedullary involvement at study entry. Grade 3-4 hematological toxicities were common (anemia- 62%, thrombocytopenia- 54%, neutropenia- 98%, lymphopenia- 100%). Cytokine release syndrome (CRS) occurred in 48/50 (96%)- grade 1/2- 17 patients (82%); grade 3- 7 patients (14%). Tocilizumab was used in 40/48 patients with CRS (median of 1 dose, range 1-4) and corticosteroids in 8/48. Two cases of immune effector cell associated neurotoxicity syndrome (ICANS) were observed (grade 1 and 2). Two patients developed CART-associated hemophagocytic lymphohistiocytosis syndrome (HLH). No irreversible organ toxicities or treatment related deaths occurred. The overall response rate (ORR) was 45/50 (90%), including 29 patients (58%) with complete response (CR)/stringent CR, 10 (20%) with very good partial response and 6 (12%) with partial response. Thirty-five patients (70%) achieved minimal residual disease (MRD) negativity at day +30. At a median follow-up of 11.9 months (range: 0.6-19), the median progression-free survival was 10.6 months, and the median overall survival was not reached (Figure 1). Although the presence of extra-medullary disease and prior anti-BCMA therapy correlated with worse outcome, high response rates were still observed (ORR of 98%/75% for patients without/with extra-medullary disease, respectively, and 95%/75% for patients without/with prior anti-BCMA therapy, respectively). Updated results will be communicated at the presentation time. CONCLUSION: Our findings demonstrate the manageable safety and high efficacy profiles of HBI0101 also in high-risk patients. Fast production time enabled the timely treatment of 98% of apheresed patients. These favorable data are encouraging and support decentralization of CART production at an academic setting, ensuring a sufficient CART supply in the light of the increasing demand.

InN@In2S3 Core-Shell nanorod for oxygen-free and penetration depth-unrestricted photodynamic therapy against glioblastoma

Photodynamic therapy (PDT) is among the most promising antineoplastic protocols for glioblastoma (GBM). Nonetheless, GBM is a deep-seated intracranial tumor with a serious hypoxic microenvironment; thus, ensuring a sufficient light penetration depth and oxygen (O2) supply are crucial to achieving a positive therapeutic outcome against GBM using PDT. Herein, InN@In2S3 core–shell nanorods are designed to serve as novel and indispensable PDT agents for GBM treatment. Unlike other PDT agents, InN@In2S3 features wide and strong near-infrared absorption that may improve the penetration depth. Moreover, InN@In2S3 exhibits high electron–hole separation efficiency owing to its Ⅱ heterojunction. The separated holes catalyze the generation of O2 from H2O, thereby ensuring sufficient O2 supply for generating reactive oxygen species through a redox reaction between the generated O2 and separated electrons. Our study demonstrates an O2-free and penetration depth-unrestricted PDT strategy that will promote the clinical treatment of GBM with PDT.

Coenzyme q10 in cardiovascular disease: an insight for a potential therapeutic benefit

The human body synthesizes the provitamin coenzyme Q10 (CoQ10), which plays a crucial role in the electron transport chain by ensuring a consistent and sufficient energy supply. CoQ10 exhibits multiple cellular roles, including its involvement in mitochondrial function, numerous cellular compartments, and plasma lipoproteins. Furthermore, CoQ10 plays a crucial role as an important antioxidant. The chemical composition of CoQ10 exhibits a strong correlation with vitamin K. It consists of a benzoquinone ring serving as the head group, accompanied by a tail made of ten isoprene units. A lack of CoQ10 has been identified as a fundamental factor contributing to numerous age-related and chronic illness problems. The efficacy of CoQ10 in various medical conditions has been proved by empirical evidence. Numerous research trials are now investigating the therapeutic and nutritional potential of CoQ10. CoQ10 continues to be predominantly utilized in the realm of cardiac research and reproductive treatments. However, there has been a notable surge of interest in exploring novel applications, notably in the context of topical administration.

Metabolomic responses based on transcriptome of the hepatopancreas in Exopalaemon carinicauda under carbonate alkalinity stress

High carbonate alkalinity is one of the major stress factors for survival of aquatic animals in saline-alkaline water. Exopalaemon carinicauda is a good model for studying the saline-alkaline adaption mechanism in crustacean because of its great adaptive capacity to alkalinity stress. In this study, non-targeted liquid chromatography-mass spectrometry (LC-MS) metabolomics analyses based on high-throughput RNA sequencing (RNA-Seq) were used to study the metabolomic responses of hepatopancreas in E. carinicauda at 12 h and 36 h after acute carbonate alkalinity stress. The results revealed that most of the significantly differential metabolites were related to the lipid metabolism. In particular, the sphingolipid metabolism was observed at 12 h, the glycerophospholipid metabolism was detected at 36 h, and the linoleic acid metabolic pathway was significantly enriched at both 12 h and 36 h. The combined transcriptome and metabolome analysis showed that energy consumption increased at 12 h, resulting in significant enrichment of AMPK signaling pathways, which contributed to maintain energy homeostasis. Subsequently, the hepatopancreas provided sufficient energy supply through cAMP signaling pathway and glycerophosphate metabolism to maintain normal metabolic function at 36 h. These findings might help to understand the molecular mechanisms of the E. carinicauda under carbonate alkalinity stress, thereby promote the research and development of saline-alkaline resistant shrimp.

Assessment of vitamin A levels in breast milk and serum of lactating mothers in Southeast Nigeria

Background: Vitamin A (VA) is an essential micronutrient critical to human health, infants rely on their mother’s breast milk for sufficient supply. This makes the maternal diet a crucial source of VA for infant growth and development. Aims: This study aimed to assess maternal VA status by assessing serum and breast milk retinol concentrations as well as dietary intake in nursing mothers. Subjects and Methods: A clinic-based cross-sectional study, conducted in Awka, south-east Nigeria, was used to assess 127 lactating women at one to 24 months post-partum. Blood and breast milk samples were obtained from each participant in a fasted state while dietary intake was assessed via an interviewer-administered semi-structured food frequency questionnaire. Retinol concentration was analyzed by spectrophotometry. Descriptive statistics were used to summarize the data while Spearman rank correlation analysis was used to determine associations between breast milk and serum retinol concentrations and study variables. Results: The mean retinol concentration in the serum of the women was a borderline value of 0.75 ± 0.64 µmol/L while the mean retinol concentration in breast milk was 0.99 ± 0.71 µmol/L. Further, the mean milk fat content was 56.18 ± 32.32 g/L while the mean milk retinol to fat ratio was 0.027 ± 0.044 µmol/g. Maternal diet consisted of a preference for provitamin A foods with low bioavailability. The mean retinol concentration in breast milk increased with time post-partum. There was no statistically significant relationship found between the frequency of consumption of most foods and the concentration of retinol in breast milk. Conclusion: The evidence of VA deficiency highlights the need for screening and nutrition education of breastfeeding mothers during ante- and post-natal visits. Keywords: Human milk, serum, vitamin A, maternal diet.

Structure-based functional analysis of a novel NADPH-producing glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum

Corynebacterium glutamicum is an industrial workhorse applied in the production of valuable biochemicals. In the process of bio-based chemical production, improving cofactor recycling and mitigating cofactor imbalance are considered major solutions for enhancing the production yield and efficiency. Although, glyceraldehyde-3-phosphate dehydrogenase (GapDH), a glycolytic enzyme, can be a promising candidate for a sufficient NADPH cofactor supply, however, most microorganisms have only NAD-dependent GapDHs. In this study, we performed functional characterization and structure determination of novel NADPH-producing GapDH from C. glutamicum (CgGapX). Based on the crystal structure of CgGapX in complex with NADP cofactor, the unique structural features of CgGapX for NADP stabilization were elucidated. Also, N-terminal additional region (Auxiliary domain, AD) appears to have an effect on enzyme stabilization. In addition, through structure-guided enzyme engineering, we developed a CgGapX variant that exhibited 4.3-fold higher kcat, and 1.2-fold higher kcat/KM values when compared with wild-type. Furthermore, a bioinformatic analysis of 100 GapX-like enzymes from 97 microorganisms in the KEGG database revealed that the GapX-like enzymes possess a variety of AD, which seem to determine enzyme stability. Our findings are expected to provide valuable information for supplying NADPH cofactor pools in bio-based value-added chemical production.