Enzymes Invertase Research Articles

Non-Saccharomyces yeasts diversity on grape berries and molecular characterization of Starmerella bacillaris yeasts strains having high invertase activity

The diversity of yeast species on grape berries changes depending on various factors. Determination of indigenous yeast intensity and diversity on grape berries is important for increasing the sensory characteristics of wine as well as fermentation efficiency. The natural yeast biota of grape berries affects wine flavour and quality by producing some secondary metabolites and hydrolytic enzymes. Despite the application of different nonconventional yeasts in food and fermentation industries, many significant researches are conducted in finding and improving the new strains having industrially important enzyme activities. Invertase enzyme has a vital role in the food industry in which it increases the sweetness of food without crystallizing them. Here, we studied yeast diversity in grape berries from selected localities and also their invertase activity. We collected grape berries from Alphonse, Kınalı Yapıncak, Çavuş, Efes Karası, Cinsaut, Atasarısı and Isabella grape varieties cultivated in Bozcaada island and Gelibolu peninsula. Twenty-one yeast species belonging to seven genera were identified. The yeast strains having high invertase activities were identified with 5.8S-ITS rDNA sequencing technique. The diversity of yeast biota on berries collected from Gelibolu was greater than that of Bozcaada. Metschnikowia pulcherrima, Cryptococcus laurentii and Rhodotorula glutinis yeast species were dominant yeast species on grape berries. A total of 294 sucrose grown yeast strains showed growth on sucrose, and 19 of them exhibit the highest invertase activity that is not glucose repressible. These 19 yeast strains were identified as Starmerella bacillaris using 5.8S ITS rDNA region and the phylogenetic analysis was inferred with the Maximum Parsimony method.

Determination of Therapeutic Values of Düzce/Yığılca Honeys by Underlining Overlooked Parameters

In this study, we aimed to determine the therapeutic value of local Yığılca honeys by examining their physicochemical characteristics, phenolic content, radical scavenging activity, and antimicrobial properties by underlining some overlooked parameters. Honey samples ware divided into two main groups as CH and FH based on melissopalynologycal analysis. Antimicrobial activity of collected honey samples were investigated on important hospital-acquired infections bacteria strains; MRSA (Methicillin-resistant Staphylococcus aureus), MSSA (Methicillin-sensitive Staphylococcus aureus), Escherichia coli, and Acinetobacter baumannii. Although CH’s antimicrobial activities were found slightly higher than the FH’s, there isn’t statistically significant differences between two type honeys. However, the antioxidant activity of CH was found significantly higher than FH. Surprisingly, a higher amount of isomaltose was determined in addition to the total phenolic content in CH compared to FH. There has been found positive correlation between isomaltose amount and zone diameters for MRSA and A. baumannii. We would like to draw attention to isomaltose for its health benefit structure since these parameters may be influence honey’s therapeutic value. We recommend that isomaltose and invertase enzyme should be included in to the honey codex standards suitably depending on the monofloral and multifloral honey’s specific structure, to sensitively standardize and control their quality and therapeutic value. Our data revealed a positive correlation between antioxidant and antimicrobial activity and total phenolic content and higher isomaltose amount.

Relationship between accumulation of autotoxins and soil fertility factors under long-term continuous cropping of cucumber in solar greenhouse

In this study, we investigated the effects of long-term continuous cucumber cropping on phenolic acids in rhizosphere soil, as well as their link to soil chemical characteristics, enzyme activities, and microbiological activities, using rhizosphere soil from the 2nd, 6th, 10th, 14th, 18th, 20th, 24th, and 26th round of cucumber cultivation in solar greenhouse. The results showed that contents of phenolic acids increased significantly with increasing continuous cropping rounds. The increase amount per round of total phenolic acid was significantly higher in the early stage (0-2 rounds) and late stage (20-26 rounds) than middle stage (10-14 rounds) of continuous cropping. Soil nutrient contents were enriched, while invertase enzyme activity and microbial activities were decreased. Redundancy analysis showed that organic matter, total phosphorus, total nitrogen, available nitrogen, microbial biomass carbon and microbial metabolic entropy were main soil fertility factors correlating with the accumulation of phenolic acids. Results of structural equation model showed that soil phosphorus enrichment directly led to the accumulation of phenolic acids, and that nitrogen enrichment indirectly facilitated the accumulation of phenolic acids by altering the activity of microorganisms. As a result, proper nitrogen and phosphorus fertilizers application would reduce the accumulation of phenolic acids and alleviate the cucumber continuous cropping obstacles.

Sugar, Invertase Enzyme Activities and Invertase Gene Expression in Different Developmental Stages of Strawberry Fruits.

The cultivated strawberry (Fragaria × ananassa) is octoploid (2n = 8x = 56) and has been the focused fruit species of which an increasing number of molecular and genetic research has been conducted in recent years. The aim of this study is to identify the relationships between sucrose metabolism, invertase enzyme activity and gene expression in four different fruit development periods (red, pink, green and white) of two commercially important strawberry varieties ‘Rubygem’ and ‘Fortuna’. The metabolite profiles (glucose, fructose, sucrose and total sugar content) of two varieties were discovered to be extremely similar. The highest amount of total sugar was found in red fruits, while the lowest was obtained from green fruits. Invertase represents one of the key enzymes in sucrose metabolism. The lowest invertase activity was obtained from the green fruits in ‘Rubygem’ and ‘Fortuna’ during four developmental periods. In these varieties, the amount of sucrose was found to be close to glucose and fructose and the lowest amount was detected in green period, while invertase activity was relatively high during red and pink periods and invertase gene expression was determined at high levels in both primers (St-4 and St-6) in the green period. The results of the study indicated that sugar content and invertase activity were positively correlated while enzyme activity and gene expression were negatively correlated.

Environmentally significant indicators of mountain meadow soils in Azerbaijan

The most widespread soils in the Republic of Azerbaijan are mountain-meadow soils. The aim of the research was a comprehensive and comparative study of morphodiagnostic parameters, physical and chemical properties, as well as the biochemical activity of natural and anthropogenically modified biogeocenoses of the Kedabey region. The area has unique flora and fauna. Surroundings of the villages Gara Murad, Kichik Garamurad, Saratovka and others were investigated. The research was carried out on virgin and cultivated lands in villages. The degree of mineralization of the rivers Zayamchay and Chekhrichay ranges from 140 to 430 mg. The groundwater level in these areas exceeds 2 m, salinization processes are active. A herbarium was collected and the floristic composition of natural cenoses was determined. Invertebrates were also collected and the dominant composition of the fauna of natural cenoses was determined. Of the saprophages in these biocenoses, woodlice of the genus Hemilepisthus and Armadillidium are widely used. The most intensive decomposition of the remains of cereal vegetation is observed in the soil of the coastal strip (65.9%). In the natural cenosis, the rate of destruction of saltwort and salt-tolerant grass vegetation reaches 44.3%. The main role in the destruction of plant remains is played by a group of saprphages, which actively use plant litter. The decomposing material reaches its final stage of microbiological transformation. The duration and amount of CO2 in different soil horizons were also studied. It has been established that with increasing depth, the amount of carbon dioxide decreases. The hydrolytic activity of invertase and urease enzymes in the studied soils can be assessed as very weak. Comparative results of all these agrochemical studies helped to develop virgin soils in agriculture on the plains and achieve high productivity.

Determination of effective assay parameters on the activity of magnetite cross-linked invertase aggregates by personal glucose meter

The invertase enzyme has been immobilized onto various support materials to enhance operational stability, shelf life, and reusability compared to free ones. Among the immobilization methods, carrier-free immobilization, such as cross-linked enzyme aggregates (CLEAs), attracts attention. However, recovery of the CLEAs from the reaction environment by centrifugation or filtration is a great challenge. The use of magnetic nanoparticles (Fe3O4) can overcome these separation limitations of the aggregates. For this aim, in this study, the surface of magnetite nanoparticles was functionalized with a significant number of free amino groups through silanization reaction. After precipitation of these aggregates and then cross-linking to the functionalized structure, the resulting magnetite cross-linked invertase aggregates (MCLIAs) were obtained, having different enzyme and glutaraldehyde concentrations. Magnetic nanoparticles were characterized by SEM, VSM, and FTIR analysis. The assay parameters, both constructional and experimental ones on the activity of MCLIAs, were practically determined by a personal glucose meter (PGM). The results indicate that magnetite nanoparticles with superparamagnetic behaviour were successfully functionalized with amino groups. The activity results demonstrated that experimental parameters were more effective than constructional parameters. MCLIAs also exhibited maximum activity at pH 5. Furthermore, 30-min incubation time and 35 °C were the best activity assay conditions.

Invertase production by yeast mediated silver nanoparticles using statistical tool central composite design

Nanotechnology is a promising field which modifies the properties of materials at the atomic level to attain specific properties with nanometer sized items. The enzyme used in the present study is invertase (beta – fructofuranosidase fructohydrolase, EC 3.2.1.26), which hydrolysis sucrose to fructose and glucose. In industry, the invertase enzyme is used for the significant production of fructose syrup from sucrose syrup. Silver nanoparticles were synthesized from baker’s yeast by bioreduction method. Invertase enzyme was extracted using these yeast mediated AgNPs from a low cost substrate (bagasse). This study focuses on optimization activity interaction of AgNPs induced enzyme invertase, including three factors: pH, temperature (°C), substrate concentration (gl−1). All experiments are designed according to response surface method (RSM) – Central Composite Design (CCD). As the result pH was identified as significant factor (p < 0.05). A maximum activity of 0.266 IUml−1 was achieved at optimum values of substrate concentration (10.29 gl−1), pH (6.3) and temperature (29.06 °C). Technically, these silver nanoparticles are immensely applied to biomedical field. Not only its application, the production process is cost-effective, compatible for medical and pharmaceutical applications as well as large scale commercial production.

Organic fertilizer activates soil beneficial microorganisms to promote strawberry growth and soil health after fumigation

Soil fumigants aim to control soil-borne diseases below levels that affect economic crop production, but their use also reduces the abundance of beneficial microorganisms. Previous studies have shown that adding various types of fertilizers to soil after fumigation can reshape the soil microbial community and regulate crop growth. We fumigated soil with dazomet (DZ) that had been cropped continuously for more than 20 years. After fumigation we applied silicon fertilizer, potassium humate organic fertilizer, Bacillus microbial fertilizer or a mixture of the last two. We studied the effects of different fertilizers treatments on the soil's physicochemical properties, enzyme activities, key soil pathogens and beneficial microbes. We found that fertilizers applied after fumigation promoted soil beneficial microorganisms (such as Fimicutes, Chloroflexi, Bacillus and Actinomadura) restoration; increased Fusarium and Phytophthora pathogen mortality, the content of ammonium nitrogen, sucrase enzyme activity; and increased strawberry fruit yield. A significant increase in strawberry yield was positively correlated with increases in beneficial microorganisms such as Gemmatimonadota, Firmicutes, Bacillus and Flavisolibacter. We concluded that organic fertilizer applied after fumigation significantly increased the number of beneficial microorganisms, improved the physicochemical properties of the soil, increased soil enzyme activities, inhibited the growth of soil pathogens to increase strawberry fruit yield. In summary, organic fertilizer activated soil beneficial microorganisms after soil fumigation, promoted soil health, and increased strawberry fruit yield.

Portable quantification of silver ion by using personal glucose meter (PGM) and magnetite cross-linked invertase aggregates (MCLIA)

Heavy metal detection is critical due to its harmful effects on human health and the ecosystem. Enzyme-based platforms attract attention for heavy metal detection such as silver, a toxic metal, due to being small, portable, and requiring only essential equipment compared with the basic analytical methods. In this study, magnetic cross-linked invertase aggregates (MCLIA) were developed for the first time as an enzyme-based signaling platform to detect Ag+ using a personal glucose meter (PGM). EDX, FTIR, and VSM results depicted that MCLIA was successfully developed and exhibits super-paramagnetism. In addition, MCLIA selectively detected the Ag+ at a sensitivity of 1.2 inhibition rate/μM in a linear range from 5 to 70 μM with a detection limit of 4.6 μM and IC50 value of 42.3 μM. These findings strongly indicate that MCLIA is applicable as a signal platform for portable quantification of other analytes that inhibits the invertase enzyme.

An approach to assess the quality of honey using partial least square method

&lt;p&gt;The objective of the present study is to obtain the quantity of honey components such as moisture, glucose, fructose and sucrose in order to access the quality of honey. The tested honey samples are authenticated if the characteristics of a pure honey. The average ratio of 56% fructose to 44% glucose, but the ratios in the individual honeys ranged from a high of 64% fructose and 36% glucose to a low of 50% fructose and 50% glucose. The contents such as fructose and sucrose in honey is due to the presence of invertase enzymes. The organic acids present in the honey is responsible for the flavor and stability against the contamination of honey due to microorganisms. The natural food items are adulterated intentionally to increase the quantity and there by the quality gets affected. The main adulterants added in honey are sucrose, corn syrup, sugar syrup and jaggery syrup. The quantification deals in finding out the amount of basic constituents present in pure honey and adulterated honey using Fourier transform infrared (FTIR) spectrometer with the multivariate analysis and validating the same using chemical analysis method. The partial least square model is used in predicting the constituents of the samples.&lt;/p&gt;

Genome-wide identification and expression profiling of invertase gene family for abiotic stresses tolerance in Poncirus trifoliata

BackgroundSucrose (Suc) hydrolysis is directly associated with plants tolerance to multiple abiotic stresses. Invertase (INV) enzymes irreversibly catalyze Suc degradation to produce glucose (Glc) and fructose (Frc). However, genome-wide identification and function of individual members of the INV gene family in Poncirus trifoliata or its Citrus relatives in response to abiotic stresses are not fully understood.ResultsIn this report, fourteen non-redundant PtrINV family members were identified in P. trifoliata including seven alkaline/neutral INV genes (PtrA/NINV1–7), two vacuolar INV genes (PtrVINV1–2), and five cell wall INV isoforms (PtrCWINV1–5). A comprehensive analysis based on the biochemical characteristics, the chromosomal location, the exon–intron structures and the evolutionary relationships demonstrated the conservation and the divergence of PtrINVs. In addition, expression analysis of INV genes during several abiotic stresses in various tissues indicated the central role of A/NINV7 among INV family members in response to abiotic stresses. Furthermore, our data demonstrated that high accumulation of Suc, Glc, Frc and total sugar contents were directly correlated with the elevated activities of soluble INV enzymes in the cold-tolerant P. trifoliata, C. ichangensis and C. sinensis, demonstrating the potential role of soluble INV enzymes for the cold tolerance of Citrus.ConclusionsThis work offered a framework for understanding the physiological role of INV genes and laid a foundation for future functional studies of these genes in response to abiotic stresses.

Effect of Acid Whey, Enzymatic Whey, Magnetized and Unmagnetized Whey on the Qualitative and Productive Traits of Date Palm

This study was conducted in one of the private orchards in the district of Abu Al-Khaseeb, Basrah province during the growing seasons 2017 - 2018 in order to examine the effect of spraying acidic whey, enzymatic whey and magnetized and non-magnetized whey on date palm cultivars Phoenix dactylifera L. agricultural (yellow Shwithi and Al-Khdrawi). A Concentration of 100% of the whey was applied to the fruits. The results of the study showed the superiority of the yellow Shwithi and Al-Khdrawi regarding the Qualitative and Productive characteristics. There was a significant difference between the date palm cultivars regarding the length, diameter, the weight of fruit, dry matter, total and reduced sugars and fruit content of invertase enzyme were recorded 33.45 mm, 20.89 mm, 9.302 g, 37.685%, 50.28%, 36.09% and 2347 units/kg/ CO2. The spray treatment was characterized by enzymatic whey in most of the study treatments of fruit length, fruit weight, total sugars and invertase enzyme. It reached 34.55 mm, 9.883 g, 56.15% and 2005.3 units/kg/CO2. The results also showed the importance of bilateral interaction between the variety and spray treatment.

Honey Mead Fermentation from Thai Stingless Bee (Tetragonula leaviceps) Honey using Ethanol Tolerant Yeast

Honey mead is a well-known conventional alcoholic beverage made by microbial fermentation of diluted honey. The selection of prospective yeasts for inoculation of honey-must with regard to honey mead quality determines the quality of mead production. The yeast consortium tolerant to ethanol stress was selected for this study using an enrichment technique. The activity of the invertase enzyme and the level of ethanol tolerance have been investigated. Thai stingless bee honey was used as a substrate, and the selected ethanol tolerant yeast consortium was used for mead fermentation. The results revealed that the PP03 had the highest invertase activity of 75.13±9.16 U/mL and the highest ethanol tolerance level of 12%. This is the first study using an ethanol tolerant yeast consortium to ferment honey mead from Thai stingless bee honey.

Enzymatic Activity of Sod-Podzolic Soil under Sosnowsky’s Hogweed

The activity of soil phosphatase, urease and invertase enzymes in sod-podzolic soil under the influence of Sosnowsky’s hogweed in the spring has been studied. In soil samples under the mono-community of Sosnowsky’s hogweed, high activity of the phosphatase and urease enzymes has been noted; the indicator of invertase activity is characterized as very weak or absent.

Utilization of banana (Musa sp.) fronds extract as an alternative carbon source for poly(3-hydroxybutyrate) production by Cupriavidus necator H16

Replacement of substrate by utilizing renewable, sustainable, abundance, and low-costs carbon sources derived from agricultural waste enables the reduction of wastes in industry and simultaneously capacitate the production of value-added products. The total amount of sugar in the banana frond extract (BFE) was 42.7 g/L, which in part consist of 10.5 g/L fructose. The effect of various BFE concentration on the PHB production was determined by conducting the fermentation utilizing BFE ranging from 10% to 50% (v/v) at 30 °C, 200 rpm for 72 h of incubation. The fermentation with 40% (v/v) BFE resulted in the highest PHB content in biomass (32.1%) and PHB concentration (0.9 g/L). Furthermore, the production of PHB was also examined by comparing the fermentation with 40% (v/v) raw and sucrase enzyme pre-treated BFE, respectively. The fructose content in the enzyme pre-treated BFE after enzymatic hydrolysis was increased to 14.6 g/L. The fermentation with 40% (v/v) pre-treated BFE showed higher PHB content in biomass (37.4%) and PHB concentration (1.3 g/L) as compared to the 40% (v/v) original BFE. The result indicates that 40% (v/v) of enzyme pre-treated BFE can be utilized as an alternative renewable and sustainable carbon feedstocks for PHB production. • BFE can be utilized as an alternative sustainable carbon source for PHB production by C. necator H16. • Effect of various BFE concentration for 10, 20, 30, 40, and 50% (v/v) on the PHB production was examined. • Fermentation with 40% pre-treated BFE showed highest PHB content in biomass (37.4%) and concentration (1.3 g/L).

Research of Original Sorts of Honey

The topicality. Honey, a natural saturated sugar solution, which consists of fructose and glucose in the amount of 65-80% of the total amount of sugars, and also contains important minor components – enzymes (diastase and invertase), organic acids (glycolic acid, acetic acid, etc.) , vitamins, phytoncides, phenolic compounds and minerals. Monofloral honeys, obtained mainly from the nectar of one plant species, are characterized by a conditionally stable composition and unique properties, which gives grounds to class them as functional products. Purpose and methods. The purpose of the work is to use melisopalinological, organoleptic and physicochemical analyses to determine reliable criteria for the authenticity of Ukrainian original sorts of honey. Results. The botanical origin of the investigated honeys has been confirmed by the study of the pollen spectrum of various honey samples by identifying the dominant pollen grains. In addition, all studied samples had their own profile of scent, tastes and consistency, a unique complex of which correlated with honey sorts. The varietal characteristics of the samples under study also influenced the physicochemical indicators, some values of which were outside the permissible limits in accordance with the national regulatory documentation. Conclusions and discussion. For original sorts of honey with varying degrees of monoflority, there is a need for further study of the physicochemical composition in connection with their possible varietal characteristics. The scientific novelty of the work lies in a pilot study of the organoleptic characteristics and physicochemical parameters of the original of honey from Ukraine, in particular pumpkin, blueberry, and echinacea. Practical use of the obtained results is possible for further authentication of coriander, goldenrod, pumpkin, blueberry, thyme, phacelia and Echinacea honey as original sorts for further study of their properties and introduction into functional nutrition.

Effect of Sugary Feeding Periods on Physicochemical Characteristics of Bees' Honey

The objective of the present study is to know effect of sugary feeding periods for honeybee colonies [beginning (FSB), mid (FSM) and end (FSE) of the flowering seasons] on some physicochemical properties of of bees' honey. All data were statistically tested using analysis of variance and discriminating analysis to distinguish between the three types of honey for parameters that can be determined easily in routine honey control. The characterization of three types of honey was carried out based on their quality parameters [Moisture, total soluble solids (TSS), electrical conductivity, Specific gravity, viscosity, pH, total acidity, free acids, lactones, glucose, fructose, sucrose, maltose content, HMF and Diastase and Invertase enzymes]. The results showed that the three honey types could not be assigned by 100% into their actual groups even when all parameters were used simultaneously in the analysis. Two samples of the honey (FSM and FSE) were also assigned. Among different parameters used moisture, TSS, viscosity, sucrose content. The results were discussed in the light of some beekeeping managements before and after harvesting of honey, and the effectiveness of the chosen parameters.

High and ultra low concentrations of Mercuric chloride initiate their specific action on binding sites of invertase and modify its interaction with sucrose

Background: Mercuric chloride is known to inhibit the activity of enzymes. It is used in homeopathy at ultra low concentration (ULC) and is known as Mercurius corrosivus (Merc cor). ULCs of Merc cor are reported to promote enzyme activity. Objective: To see whether the mother tincture (?) of Merc cor and its ULCs interact with an enzyme invertase at its binding sites and influence enzyme’s action on its substrate sucrose. Methods: Merc cor ? (0.15 M HgCl2) was diluted with deionized and distilled (DD) water 1:100 and succussed 10 times to prepare Merc cor 1 cH or 1st potency. This potency was further diluted and succussed in 200 and 1000 steps to prepare 200cH and 1000cH potencies, respectively. Merc cor 200 cH and 1000cH were prepared in 90% ethanol. The two potencies and blank 90% EtOH were diluted with DD water 1:1000 to minimize ethanol content to a negligible amount 0.09%. The control was DD water (55mM). The drugs, EtOH and water control were mixed separately with 0.037 mM invertase in DD water. Using an isothermal calorimetry (ITC) instrument the substrate sucrose (65mM) was injected at 2 µl every 2 min into 300 µl invertase solution 20 times at 25 0C. Molecular modeling study was done to predict possible binding sites and nature of binding between the enzyme and HgCl2 and between the enzyme and water. Potencies after dilution are virtually water. Fluorescence spectra of invertase (4µM) mixed with drug/control solutions were also obtained to see the effect of drugs on protein folding. Results: Thermodynamic parameters like binding constant, change in enthalpy, entropy and Gibbs free energy showed marked variation in treatment effects on the enzyme. Molecular modeling study also shows variation in binding between invertase and HgCl2 and between invertase and water. Fluorescence spectra show variation in quenching related to different treatments. Conclusion: Merc cor mother tincture and its potencies interact at different binding sites of invertase and modify the enzyme’s action on sucrose. Drug solutions induce conformational changes in the enzyme.

Влияние гуминового препарата «BIO-Дон» на содержание элементов питания в почве и качество черешни

The work deals with the study of the influence of humic preparation ‟BIO-Don” on indicators of soil fertility, the enzymatic activity of Сalcic Chernozem (Pachic) at the experimental site, as well as on the productivity and quality of fruit crops, for example, cherries varieties Talisman and Vasilisa in terms of work experience in the open ground in the territory of «Agrofirm “the Red garden”». Treatment of fruit trees was carried out once by drip irrigation in a dosage of 300 l/ha of working solution with a concentration of gp 0.008 g/l for carbon, soil samples were taken before treatment, two weeks and a month after the application of the humic preparation, a site without the use of “BIO-Don” was used as a control option. The treatment of plants with a humic preparation increases the activity of the enzymes catalase, invertase and phosphatase, especially during the period of mass ripening of fruits. Accordingly, at the same time, the content of mobile phosphorus and exchangeable potassium decreases, which is due to the increased removal of these elements by plants. Chemical analysis of fruit products for the content of soluble solids and titrated acidity revealed a statistically significant increase in these indicators in fruits from areas treated with humic preparation, which is manifested by their greater taste saturation.

Ultimate fate and toxicological consequences of insecticide pyriproxyfen and its metabolites in soil ecosystem

Insecticide pyriproxyfen (PYR) is very specific to insect pests and possess low mammalian toxicity. Its degradation can result in formation of about 10 metabolites in the environment. Some of the metabolites are reported to be very toxic and mobile in nature. Their potential to cause toxicity through environmental contamination may raise serious concern. Available literature is short of the information on formation of different metabolites in soil, their ultimate fate and toxicological consequences. We studied metabolic pathway of PYR by observing dissipation behavior of different metabolites in soil under sub-tropical agro-climatic conditions of north India. Soil under field conditions was treated with PYR at (T1) 100 and (T2) 200 g a.i./ha. Samples were drawn periodically, processed and analyzed using GC-MS tandem mass spectrometry. Six metabolites namely 4-OH-PYR, POP, POPA, 4-OH-POPA, PYPA and PYPAC were formed during degradation process. Most metabolites appeared very early and attained maximum concentration on day one of PYR application. However, their residues persisted for more than 30 days with variable half-lives ranged from 2.6 to 30 days. The toxicological study revealed that metabolites C, E and F were highly pernicious to soil enzymes sucrase, catalase, urease and dehydrogenase. PYR do not posed any detrimental effects in adult honeybees. Considering the persistence behavior and toxicological consequences of metabolites, further investigations are needed for preventing their harmful effect on non-target organisms and associated environment.Summary: Degradation of pyriproxyfen revealed 6 metabolites most of which persisted for more than 30 days in soil. Metabolites C, E and F caused toxicity to soil enzymes. Parent PYR do not caused any detrimental toxicity effect in adult honeybees.