Successive Harvests Research Articles

Numerical investigation of future potato yields in Bangladesh using Machine learning and IoT

The changing environment has significantly affected Bangladesh for the past several decades, and a number of minerals and nutrients are in short supply in the human population. However, not only are we being affected by the effects of climate change, but also the crops cultivated on our farms are feeling its impact. The nutrient content and growth of food crops are affected by many components of climate change, such as rising temperatures, increased precipitation, rising CO2 levels, etc. It is commonly regarded that potatoes are one of the healthiest and most popular foods in Bangladesh, with an abundance of nutrients and electrolytes that are beneficial to the body. However, in the not-too-distant future, the production of potatoes will be affected by the high temperature and decreased precipitation resulting from climate change. As potatoes are one of the most essential staple foods in Bangladesh, a viable solution must be found for continuous potato production in the country. A comprehensive examination of the evolution, production, and important nutrients of potatoes will be performed in this study. A range of sensors, networks, drones, cameras, and applications will be collected for the purpose of monitoring the potato field and ensuring that the crops are cultivated in an acceptable environment. This idea can aid our country economically and agriculturally by increasing potato production. Potatoes are affordable for consumers from all socioeconomic classes. Through this idea, Producers will achieve a successful harvest of nutrient-rich potatoes for public consumption.

Development and assessment of Nirgundi-Chakramardha biopesticide against Diamondback Moth on Cabbage

Introduction: The global population is rapidly approaching 9.7 billion by 2050, with Africa and Asia playing significant roles in this growth. This surge necessitates increased crop production, highlighting the importance of effective pest control to ensure food security. India, facing an annual crop loss of about 30% due to pests and diseases, emphasizes the urgency of maintaining ecological balance in natural ecosystems. The diamond back moth (Plutella xylostella L.) poses a substantial threat to cruciferous plants globally, causing severe yield losses of 50–80% in infested cabbage. Addressing this challenge is crucial for minimizing crop losses and securing successful harvests. Our study aimed to analyze the emergence and abundance of small, medium, and large-sized larvae before and after applying treatments. Materials and Methods: A study with five treatments, including Nirgundi-chakramardha biopesticide and a control (Cypermethrin), was conducted. Each treatment was replicated four times, and the entire experiment was set up using a randomized block design. Results: After chemical application, all treatments displayed significant differences. The biopesticide in our study produced notable results within trial groups. Notably, the control group exhibited excellent results compared to the other treatments. Conclusion: The Nirgundi-chakramardha Biopesticide exhibited insecticidal activity against Diamondback moth pests, specifically targeting medium-sized larvae. Although not statistically significant compared to the control group, its efficacy in pest control, especially during the larval stage, indicates its potential as a viable pest management option. Further research and optimization could improve its effectiveness in combating Diamondback moth infestations.

Broad geographic variation in age- and sex-dependent origin of harvested eurasian wigeon (Mareca penelope) revealed by stable-hydrogen (δ2H) isotope analyses of feathers

Migratory waterfowl are a harvested resource shared among multiple European countries, exposing them to potential overexploitation. Management of take is challenging since the life cycle of migratory waterfowl consists of several stages distributed among several locations, with possible spatio-temporal overlap among populations with differing population trends. Successful harvest management in such situations requires knowledge about the connections between breeding and non-breeding locations, and where birds are harvested. Breeding populations of Eurasian wigeon (Mareca penelope) are declining in Finland, underlining the need for more effective harvest management. Relative proportions and temporal distribution of local breeding birds and migrants from a larger Russian breeding population within the Finnish hunting bag has been unknown to date. We studied spatio-temporal origins of Finnish harvested wigeon by measuring stable-hydrogen (δ2H) isotope values from legally harvested birds. We modelled the changes in δ2H values of the feather samples within the hunting season using Gaussian processes and found that the origin of harvested wigeon in Finland changed during the hunting season and differed by age and sex. In juveniles and adult females but not in adult males, origin of harvested birds shifted from local and possibly western Russian birds to more long-distance migratory birds during the harvest season. These patterns likely reflected sex- and age-specific differences in migratory behaviour of Eurasian wigeon in the East Atlantic flyway, which can be used to guide future management and conservation of this species through the implementation of spatio-temporal harvest regulation.

The Belief in Pullayar among Agrarian People in Ampara District, Sri Lanka

Worship of deities takes a specific position among the religious beliefs related to agriculture. The Pullayar deity belief with agriculture specially can be seen in Northern Province and Nuwarakalaviya in the North Central Province of Sri Lanka. The study investigates the significance of the Pullayar deity belief among the agricultural community in Ampara District. It also focusses on the ways in which the agricultural practices of farmers, socio-cultural identities, and customs are influenced by the deity belief system. The main objective of the research was to examine the contemporary condition of Pullayar deity belief associated with agriculture in the Ampara District. The two Grama Niladari (GN) divisions of Galahitiyagoda and Udayagiriya belonging to Uhana Divisional Secretariat in Ampara district were used as the study areas. Data required for the research was collected using forty (40) farmers selected from the study area under random sampling method. Participatory observation and unstructured interviews were used to gather data. Pullayar worship is closely linked with the agricultural practices of Ampara district. Here farmers perform specific rituals for a successful harvest. Among the farming folk beliefs of Ampara, the Pullayar deity worship is uniquely adapted to protect the land from fertility and natural calamities. According to the farmers in this area, it is customary to worship the Buddha as well as Gambara Devi before doing any task in the paddy fields. As a special offering for pullayar deity, farmers offer coconut and let the pots overflow with milk as a ritual. It is performed by them for the protection of their fields and cattle. Pullayar rituals foster collective social cohesion. Strengthens community bonds through shared spiritual practices. Worship as well reveals a gendered division of religious duties, with men leading agricultural rituals and women maintaining household shrines. Therefore, this society reflects a balanced role of spiritual practices across genders. Though, Pullayar deity worship can be regarded as a particular cultural aspect that is unique to the agrarian economic system in this area and still exists now.

Tailored thermoelectric performance of poly(phenylene butadiynylene)s/carbon nanotubes nanocomposites towards wearable thermoelectric generator application

In this study, two conjugated polymers (CPs) featuring poly(phenylene butadiynylene) (PPB) were meticulously synthesized and composited with single-walled carbon nanotubes (SWCNTs) to investigate their thermoelectric properties and fabricate wearable thermoelectric generators (TEGs). These CPs, designated as P1 and P2, were tailored with distinct side chain configurations by incorporating 2-butyloctyloxy and 6-(methyldioctylsilyl)hexyloxy groups, respectively. Notably, P2, characterized by longer side chains with branchpoints farther from the backbone, exhibited planar backbone structure and enhanced solubility, consequently engendering stronger π–π interaction with SWCNTs and facilitating the disperse of SWCNTs through polymer wrapping at bundle surface. Such characteristics therefore contributed to the superior thermoelectric performances of the P2/SWCNTs nanocomposite, yielding a higher power factor (PF) of 216.5 μW m−1 K−2 for the spin-coated film. Furthermore, the corresponding wearable TEGs, which were constructed through spray-coating with 14 legs, resulted in an output power of approximately 49.6 nW under a temperature difference of 25 K, exhibiting successful harvesting of waste heat. Further applications also demonstrated operational efficacy under diverse conditions. These findings not only underscored the significance of side chain engineering in tailoring the thermoelectric properties of CP/SWCNTs nanocomposites but also demonstrated the feasibility of fabricating high-performance wearable thermoelectric devices applicable in versatile contexts.

Ethanol and Gd3+ activated aggregation induced delayed fluorescence in copper nanoclusters for detection of Cr(VI)

In recent years, significant emphasis has been dedicated towards investigating aggregation-induced emission (AIE) phenomena, and a notable addition to this emerging field is aggregation-induced delayed fluorescence (AIDF). Here, we proposed two different strategies to produce AIDF-based luminescent materials at room temperature from glutathione-capped CuNCs (GSH-CuNCs) by (1) simply modifying the solvent environment and (2) introducing gadolinium (Gd3+) ions. The synthesized GSH-CuNCs displayed weak fluorescence (Fl) emission in aqueous solution with a short delayed lifetime of 3.2 μs and quantum yield (QY) of only 1.42 %. However, introducing GSH-CuNCs to an ethanol medium promptly led to enhanced delayed Fl emission with a significantly delayed lifetime of 21.8 μs and a high QY of 89.2 %. Moreover, introducing Gd3+ ions to the aqueous GSH-CuNC solution also enhances delayed Fl emission with a delayed lifetime of 13.6 μs and a high QY of 79.5 %. Analysis of transmission electron microscopy and dynamic light scattering data showed that both the ethanol medium and Gd3+ addition endows controlled aggregation of GSH-CuNCs, enabling successful harvesting of triplet states and ultimately leading to the AIDF phenomenon. Moreover, the AIDF harnessed from GSH-CuNCs by Gd3+ was successfully employed to detect Cr6+ ions in an aqueous solution with excellent selectivity.

Use of Perpendicular Plate of Ethmoid Split Technique in Rhinoplasty and Septoplasty.

In recent years, the perpendicular plate of ethmoid (PPE) has become a prominent choice for bone-grafting materials. This study introduces the PPE split technique for efficient removal of the bony septum or the harvesting of partial PPE for septal batten grafts in rhinoplasty and septoplasty, particularly suited for cases with thick PPE. Herein, this technique was employed to minimize septal defects and prevent complications while achieving harvested bony grafts of the desired effective thickness. In this retrospective study, 36 patients underwent surgery using the PPE split technique (22 septoplasties and 14 rhinoplasties). The procedure involved detaching the PPE from the septal cartilage and performing a vertical plane osteotomy with a number 10 scalpel blade to remove bony spurs or prepare batten grafts. Paranasal sinus CT revealed that the average anteroinferior PPE thickness was 3.46 mm. This technique was used for both bony batten graft harvesting (26 cases) and bony spur removal, and septum reshaping (10 cases). Successful PPE harvesting was achieved in all cases except for three, which experienced graft breakage. This study demonstrates the viability and effectiveness of the PPE split technique as a supplementary maneuver in septoplasty and rhinoplasty procedures. Its diverse applications include reshaping, correcting deviated septum, harvesting bony batten grafts, and providing support for an aesthetically pleasing nasal profile.

Characterization of early maturing elite genotypes based on MTSI and MGIDI indexes: an illustration in upland cotton (Gossypium hirsutum L.)

BackgroundGlobally, the cultivation of cotton is constrained by its tendency for extended periods of growth. Early maturity plays a potential role in rainfed-based multiple cropping system especially in the current era of climate change. In the current study, a set of 20 diverse Gossypium hirsutum genotypes were evaluated in two crop seasons with three planting densities and assessed for 11 morphological traits related to early maturity. The study aimed to identify genotype(s) that mature rapidly and accomplish well under diverse environmental conditions based on the two robust multivariate techniques called multi-trait stability index (MTSI) and multi-trait genotype-ideotype distance index (MGIDI).ResultsMTSI analysis revealed that out of the 20 genotypes, three genotypes, viz., NNDC-30, A-2, and S-32 accomplished well in terms of early maturity traits in two seasons. Furthermore, three genotypes were selected using MGIDI method for each planting densities with a selection intensity of 15%. The strengths and weaknesses of the genotypes selected based on MGIDI method highlighted that the breeders could focus on developing early-maturing genotypes with specific traits such as days to first flower and boll opening. The selected genotypes exhibited positive genetic gains for traits related to earliness and a successful harvest during the first and second pickings. However, there were negative gains for traits related to flowering and boll opening.ConclusionThe study identified three genotypes exhibiting early maturity and accomplished well under different planting densities. The multivariate methods (MTSI and MGIDI) serve as novel approaches for selecting desired genotypes in plant breeding programs, especially across various growing environments. These methods offer exclusive benefits and can easily construe and minimize multicollinearity issues.

Interspecific Hardy Geranium Progenies: Morphological Characterization and Genetic Evaluation

Hardy geranium is a popular ornamental plant with a high market demand. As a result, there is a strong need for continuous innovation to improve its ornamental qualities. Interspecific hybridization is a widely used technique for introducing desirable traits and creating new cultivars with added value. To explore the possibilities of creating novelty within the Geranium genus, we conducted 2438 interspecific crosses over three flowering seasons, resulting in the successful harvest of 445 seeds and the final production of 82 seedlings. Using embryo rescue increased the germination rate of a seedling from 0.17% to 1.01%. To validate the paternal genetic contribution in the new seedlings, we used amplified fragment length polymorphism (AFLP) to assess the proportion of uniquely inherited paternal markers in all seedlings. AFLP analysis confirmed that 54 of the 82 seedlings were indeed true hybrids (65.8% hybridization success rate). A morphological evaluation of the flowers and leaves, including flower diameter (mm), style length (mm), number of flowers, flowering period (weeks), and color of both flowers and leaves, provided further confirmation of the F1 hybrid status of the seedlings. The results of our interspecific breeding demonstrated the efficiency of interspecific hybridization in hardy geraniums and its potential to create distinctive and novel cultivars for the ornamental market.

3D Camera and Single-Point Laser Sensor Integration for Apple Localization in Spindle-Type Orchard Systems.

Accurate localization of apples is the key factor that determines a successful harvesting cycle in the automation of apple harvesting for unmanned operations. In this regard, accurate depth sensing or positional information of apples is required for harvesting apples based on robotic systems, which is challenging in outdoor environments because of uneven light variations when using 3D cameras for the localization of apples. Therefore, this research attempted to overcome the effect of light variations for the 3D cameras during outdoor apple harvesting operations. Thus, integrated single-point laser sensors for the localization of apples using a state-of-the-art model, the EfficientDet object detection algorithm with an mAP@0.5 of 0.775 were used in this study. In the experiments, a RealSense D455f RGB-D camera was integrated with a single-point laser ranging sensor utilized to obtain precise apple localization coordinates for implementation in a harvesting robot. The single-point laser range sensor was attached to two servo motors capable of moving the center position of the detected apples based on the detection ID generated by the DeepSORT (online real-time tracking) algorithm. The experiments were conducted under indoor and outdoor conditions in a spindle-type apple orchard artificial architecture by mounting the combined sensor system behind a four-wheel tractor. The localization coordinates were compared between the RGB-D camera depth values and the combined sensor system under different light conditions. The results show that the root-mean-square error (RMSE) values of the RGB-D camera depth and integrated sensor mechanism varied from 3.91 to 8.36 cm and from 1.62 to 2.13 cm under 476~600 lx to 1023~1100 × 100 lx light conditions, respectively. The integrated sensor system can be used for an apple harvesting robotic manipulator with a positional accuracy of ±2 cm, except for some apples that were occluded due to leaves and branches. Further research will be carried out using changes in the position of the integrated system for recognition of the affected apples for harvesting operations.

Cartilage Warping in Rhinoplasty: An Assessment of Temperature, Carving, and Suturing Conditions

Abstract Goals/Purpose Rhinoplasty is often contingent on the successful harvesting and allocation of cartilage in order to build the nasal framework. While autologous and cadaveric cartilage grafts have been utilized, it remains unclear how factors, such as suturing and carving techniques, as well as temperature, impact its short- and long-term warping potential. Our systematic review seeks to objectively assess how various carving (i.e. peripheral versus central, eccentric versus concentric), suture patterns (i.e. counter-balancing, oppositional), and temperature conditions impact the warping potential of cartilage grafts in rhinoplasty. In conducting this study, we hope to determine the optimal conditions for stable cartilage constructs in rhinoplasty. Methods/Technique A systematic literature review was conducted using a combination of terms including, “warp,” “rhinoplasty,” “cartilage,” “suture,” “temperature,” “carving,” and “cutting.” Removal of duplicates was performed, followed by further screening by abstract and full text. To reduce the possibility of bias, a standardized form was used for data extraction; variables including author, year of publication, journal of publication, study design, conditions, and results were recorded. Results/Complications A total of eleven studies were included in the analysis. Cartilage types included fresh frozen cadaveric graft, autologous costal cartilage, and bovine rib cartilage. Conditions associated with reduced warping include central and concentric based carving patterns, oppositional suturing; Peripherally and eccentric cut cartilage, as well as increased time, were associated with a higher degree of warping. Conclusion To the authors’ knowledge, our systematic analysis is the first to summarize the impact of various conditions on cartilage warping in rhinoplasty. Moreover, we determine the protective nature of certain carving and suturing methods - particularly central, concentric carving, and oppositional suturing - in minimizing cartilage warping. With this information, we hope to guide plastic surgeons in optimizing conditions to potentially prevent cartilage warping, and ultimately, provide reliable and sustainable results for patients undergoing rhinoplasty.

Phase II single-arm study of a combination of obinutuzumab and venetoclax in early relapsed or refractory diffuse large B-cell lymphoma—final results of the AGMT NHL15B study

BackgroundPatients with diffuse large B-cell lymphoma (DLBCL) relapsing early (within 12 months) or primary refractory to induction therapy with rituximab (R) and CHOP have a poor prognosis. We therefore initiated a study with obinutuzumab and venetoclax.Study design and methodsTwenty-one patients with DLBCL (relapsed within 12 months or primary refractory), detectable Bcl-2 protein expression, and CD20 positivity were included in this prospective single-arm study between 2016 and 2021. Obinutuzumab was administered i.v. at a dose of 1,000 mg on days 1, 8, and 15 in cycle 1 and on day 1 of each of the following 21-day cycles. Venetoclax was given at 800 mg daily p.o. continuously. Treatment was repeated for up to three cycles. Eligible patients were planned to either proceed to cellular therapies or receive up to nine cycles of maintenance. The primary endpoint was objective response rate (ORR) after three cycles (Eudract Nr. 2016-001760-10 and NCT02987400).ResultsTwenty-one patients (median age, 64 years) with refractory or early relapsed DLBCL after one (N = 11) to four previous lines of therapy were included. The majority of patients received three cycles of obinutuzumab/venetoclax (range, 1–8). The regimen was well tolerated with manageable cytopenias and infections. Severe adverse events related to treatment were observed in 9.5%. The ORR was 38.1% (8/21 patients) with a best response of five complete remissions (CRs; 23.8%) and three partial remissions (PRs; 14.2%). The primary endpoint (45% ORR) was not met. Response duration was 83.3% at 84 days, with a progression-free survival of 38.8% at 84 days and 25.9% at 168 days and a median overall survival of 169.1 weeks. All deaths were due to underlying disease. Seven patients became eligible for autologous transplant. Overall, nine patients (42.8%) received 11 cellular therapies (5 ASCT and 6 CAR-T). Three patients went directly from obinutuzumab/venetoclax to CAR-T therapy. All patients had successful peripheral stem cell or T-cell harvests. Characteristics of responders include relapsed disease (response rate, 6 of 11 = 54%), very good or good R-IPI (7 of 8), and low number of previous therapies (median = 1).ConclusionObinutuzumab/venetoclax represents an effective chemo-free relapse regimen with low toxicity that can be followed by cellular therapies, particularly CAR-T cells.

On-demand plerixafor added to high-dose cyclophosphamide and pegylated recombinant human granulocyte colony-stimulating factor in the mobilization of patients with multiple myeloma: a treatment with high effectiveness, convenient, and affordable cost.

The combination of high-dose cyclophosphamide (HD-Cy) (3g/m2) plus granulocyte colony-stimulating factor (G-CSF) and on-demand plerixafor (PXF) has been considered an effective mobilization regimen of patients with multiple myeloma(MM). However, the daily multi-injection regimen of G-CSF poses challenges. This study delves into the efficiency and cost implications of a novel approach, using HD-Cy alongside pegylated G-CSF (PEG G-CSF) and on-demand PXF. Unlike G-CSF, which necessitates daily injections, the half-life of PEG G-CSF extended allows for a single injection. A retrospective analysis was conducted on 350 MM patients, which were categorized based on their mobilization regimens: Cy+PEG G-CSF+/-PXF (n=66), Cy+PEG G-CSF (n=91), Cy+ G-CSF (n=169), and G-CSF+PXF (n=24). Mobilization with Cy+PEG G-CSF+/-PXF(8.79)yielded a notably higher median CD34+ cell count compared to the other regimens: Cy+PEG G-CSF(4.96), Cy+G-CSF (4.65), and G-CSF+PXF (2.99) (P<0.001). The percentage of patients who achieved >6×106/kg CD34+ cells was significantly higher in the Cy+PEG G-CSF+/-PXF group (77.3%) than in the other mobilization regimens: Cy+PEG G-CSF (41.8%), Cy+ G-CSF (37.3%), and G-CSF+PXF (8.3%) (P<0.001). From a cost perspective, the Cy+PEG G-CSF+/-PXF approach was more economical than the G-CSF+PXF strategy but was marginally costlier than the other two methods. A multivariate assessment highlighted that the combination of Cy+PEG G-CSF with on-demand PXF had a superior potential to achieve the desired harvest (6×106/kg) compared to the Cy+PEG G-CSF protocol without PXF. The incremental cost-effectiveness ratio for each 1% increase in the probability of achieving a successful optimal harvest was $ 97.02 per patient. The incidence of neutropenic fever was 3.0% in the Cy+PEG G-CSF+/-PXF group. The combination of on-demand PXF with HD-Cy and PEG G-CSF offers a cost-effective approach with a high mobilization success rate, manageable side effects, and the convenience of fewer injections. It stands as a promising mobilization strategy for MM patients.

Using Paper as a Biomimetic Fog Harvesting Material

AbstractThis study identifies important factors for designing an effective biomimetic paper‐based fog harvesting substrate by examining the harvesting properties of different surfaces, including glass, polyethylene, and superhydrophobic paper. In laboratory‐scale fogging tests, the wetting behavior of the substrates is characterized, and the importance of the tilt angle of the respective surface relative to the fog flow is elaborated. Because successful fog harvesting requires both efficient accumulation of water droplets on the surface (by condensation and collision) and sufficient but not excessive roll‐off of the liquid, the amount of water finally collected is clearly related to the pinning effect, which should prevent the smallest droplets from being carried away by the wind but must not lead to full and permanent wetting of the surface. Coalescence is identified as a major phenomenon to improve droplet roll‐off. In this context, superhydrophobic paper indicates to be a more effective water collector than glass or polyethylene, especially when oriented vertically, since it allows the droplets to roll off very efficiently. Finally, the addition of glass particles to the superhydrophobic coating is proposed as a means of enhancing pinning and improving the fog harvesting efficiency.

AVALIAÇÃO QUANTITATIVA DE ÁCIDOS AMARGOS, XANTHOHUMOL E ÓLEOS ESSENCIAIS PRESENTES EM FLORES DE DIFERENTES CULTIVARES DE Humulus lupulus L. PRODUZIDAS NA REGIÃO NORDESTE DO BRASIL

QUANTITATIVE EVALUATION OF BITTER ACIDS, XANTHOHUMOL AND ESSENTIAL OILS PRESENT IN FLOWERS OF DIFFERENT CULTIVARS OF Humulus lupulus L. GROWN IN NORTHEASTERN BRAZIL. Hop flowers contain chemical substances that are used to confer sensory properties to beer. Hop flower products have very high commercial value, but national production represents less than 2% of domestic consumption. In this work, flowers from different hop cultivars grown in northeast of Brazil (Rio Grande do Norte and Ceará) were analyzed to quantitate metabolites of industrial interest. Flowers from the initial and three consecutive harvests were collected, and bitter acids and xanthohumol were evaluated by HPLC-UV and essential oils by GC-MS. The cultivar Columbus was subjected to only 10% of the irrigation time during the third flowering phase to evaluate the effect of water stress on the formation of metabolites. Compounds were detected in appreciable quantities in all samples, and consecutive flowering of some cultivars showed a significant increase in total bitter acids with successive harvests. Plants subjected to water stress showed an abrupt decrease in the concentration of compounds. The cultivars evaluated by liquid and gas chromatography showed a significant concentration of bitter acids and essential oils within the expected range, and the chemical composition of the oils showed different compounds characteristic of hops.

Establishment of competition in the market of seed and planting material certification services in Ukraine

The quality and varietal purity of seeds is one of the crucial factors in determining the consumer value of any end product. Sowing with high-quality seeds ensures a successful harvest in the future. The purpose of this study was to determine the state (formation and development) of competition in the market for certification of plant variety seeds. The main methods used in the study are statistical analysis, comprehensive assessment, deduction, abstraction, forecasting, historical, and tabular methods. This study investigated the state of competition of subjects of appraisal activity by six objective indicators, analysed their material and technical base, and human potential. The market for seed certification services is a market with limited access and should be characterised as an oligopoly. This market is represented by entities of different forms of ownership and management, it is competitive in determining seed quality and virtually monopolistic in determining varietal properties. Fluctuations in the cost of services are insignificant (15%) and are not currently the main criterion for seed producers when choosing an organisation that conducts seed certification. The present paper was the first to determine the total market capacity in terms of volume and value, and to present possible ways of its growth, which is the practical value of this study. The data presented in this study can be used by other researchers in their scientific work related to this market or agriculture. The findings may also be required by government officials or businesses operating in the market.

Surface to Perforator Index: Assessing the Importance of the Number of Perforators in Successful Harvesting of the Anterolateral Thigh Flap.

The use of perforator flaps has become more popular with improvement of surgical technique, technology, as well as understanding of microvascular anatomy. The selection of well-perfused angiosomes is critical to the successful outcome of patients undergoing free tissue transfer. The number of perforators that are needed is dependent upon the surface area of the flap being harvested; however, there have been no studies to assess the optimal surface area supplied by each perforator. We hypothesized that the smaller the surface area supplied by each perforator correlated with fewer flap-related complications in the harvesting of the anterolateral thigh (ALT) flap. All ALT flaps harvested from 2015 to 2021 at our institution were retrospectively reviewed. The surface area of the flap harvested was calculated as A = πab, where a is the long radius and b is the short radius of the ellipse. The surface-perforator index (SPI) was calculated for each flap by dividing the surface area of the ALT flap by the number of perforators supplying the flap. Our primary outcomes were flap-related complications that included: partial flap loss, dehiscence, and venous congestion. A total of 106 patients were identified. Twenty-four patients (22.6%) developed perforator-related complications. An increasing SPI and SPI to body surface area were strongly associated with development of complications (adjusted odds ratio [95% confidence interval], adjusted p: 1.02 [1.01, 1.03], < 0.001and 1.23 [1.12, 1.42], p < 0.001). An SPI of greater than 150 cm2/perforator was associated with a higher probability of complications (p < 0.001). Flap-related complications are significantly related to the number of perforators supplying the flap. The smaller the surface area supplied by a single perforator correlates with significantly fewer flap-related complications. SPI is a new index that may be used as a predictive tool to aid in identifying flaps that may be more prone to complications in free tissue transfer.

GROWTH PERFORMANCE, PARTITION COEFFICIENT AND HARVEST INDEX OF WIDELY GROWN POTATO (SOLANUM TUBEROSUM L.) VARIETIES IN ETHIOPIA

Aim of the study: Aim of the study: The main objective is to compare the differences in growth performance, dry matter partitioning coefficients and harvest index of widely grown potato varieties Material and methods: Materials and Methods: The trial was conducted at Adet Agricultural Research Center, Ethiopia during 2019/2020 and 2020/2021 main cropping season with randomized complete block design with Shenkola, Belete, Gudenie, Guassa, Dagim and Ater Ababa potato varieties. Four different harvesting dates were undertaken (30, 45, 60 and 75 days after planting (DAP). Results and conclusions: Materials and Methods: The trial was conducted at Adet Agricultural Research Center, Ethiopia during 2019/2020 and 2020/2021 main cropping season with randomized complete block design with Shenkola, Belete, Gudenie, Guassa, Dagim and Ater Ababa potato varieties. Four different harvesting dates were undertaken (30, 45, 60 and 75 days after planting (DAP). Results and conclusion At early stages of development, the highest percent of dry matter accumulation went to vegetative growth, and then to tubers in the successive harvests. The maximum tuber yield, bulking rate and harvest index (36.17 t/ha, 4.23 gplant-1day-1 and 0.8) respectively was recorded from Belete variety. Belete variety can give reasonable yield after 45 DAP. Guassa variety (having a PC of 0.92 at 75 DAP) was able to produce 92% of its yield at 75 DAP and its yield was comparable with the highest yielder (Belete variety). In such areas where erratic rainfall is expected, Guassa and Belete varieties can be used as early variety in potato production systems.

Peduncle collision-free grasping based on deep reinforcement learning for tomato harvesting robot

Collision-free grasping of the thin, brief peduncles connecting cherry tomato clusters to the main stem was crucial for tomato harvesting robots. Recognizing that the optimal operating posture for each individual peduncle was various, this study proposed a novel peduncle grasping posture decision model using deep reinforcement learning (DRL) for tomato harvesting manipulators, to overcome the collision issue caused by fixed-posture grasping. This model could dynamically generated action sequences for the harvesting manipulator, ensuring that the end-effector approach to the peduncle along the collision-free path with the optimal grasping posture. Building upon prior research into the multi-task identification of tomato clusters, peduncles, and the main stem, a keypoint-based spatial pose description model for tomato bunches was devised. Through this, the optimal operating posture for the end-effector on the peduncle was established. An improved HER-SAC (Soft Actor Critic with Hindsight Experience Replay) algorithm was subsequently established to guide the end-effector in collision-free grasping motions. The reward function of this algorithm incorporated end-effector posture constraints obtained from the optimal posture plane. In the training phase, a heuristic strategy model, providing prior knowledge, was merged with a dynamic gain module to sidestep local optimal policies, collectively enhancing the learning efficiency. In the simulation, our method improved the success rate of the peduncle grasping by at least 14 %, compared with SAC, HER-DDPG and HER-TD3. For the identical scenarios, improved HER-SAC reached the desired posture with a minimum of 15.5 % fewer steps compared to other algorithms. In field experiments conducted in tomato greenhouses, the robot achieved a harvesting success rate of 85.5 %, which was an increase of 57.3 % and 43.0 % compared to traditional methods with fixed horizontal and parallel-to-main-stem postures, respectively. The average operation time, from identification to successful harvesting, was 11.42 s. Our findings offer a promising solution to enhancing the efficiency of tomato-harvesting robots.

A Phase I Trial of Activated Donor-Derived Marrow Infiltrating Lymphocytes to Treat Relapsed Hematologic Malignancies after Allogeneic Transplantation with Post-Transplantation Cyclophosphamide As Graft-Versus-Host Disease Prophylaxis

Background: Donor lymphocyte infusion (DLI) or a second allogeneic transplant (HCT) are the only curative treatments for hematologic malignancies relapsing after initial HCT. Both approaches lack significant efficacy and may cause considerable toxicity. Post-transplant cyclophosphamide (PTCy) used as graft-versus-host disease (GVHD) prophylaxis eliminates alloreactive T cells and enriches for tumor-specific T cells in the bone marrow. We hypothesized that expanded, donor-derived marrow infiltrating lymphocytes (alloMILs) collected post-HCT could restore antitumor immunity in relapsed recipients without the additional GVHD and toxicity seen with DLI or second HCT. Methods: We conducted a phase I dose-escalation trial (NCT02342613) of expanded alloMILs to treat bone marrow infiltrating hematologic malignancies relapsing after HCT with PTCy. Key inclusion criteria were age ≥ 18 years; relapsed hematologic malignancy ≥ 6 months after HLA matched or mismatched HCT with PTCy; donor CD3 + chimerism ≥ 30% in the peripheral blood; and lack of immunosuppressant use for 2 weeks prior to alloMILs harvest. Enrolled patients underwent salvage chemotherapy prior to or after alloMILs harvest to control the disease during the expansion process. Harvested bone marrow mononuclear cells were co-incubated with anti-CD3/CD28 beads and IL-2 for 7 to 14 days until the target CD3 + dose was reached. Given the differential risk of GVHD with matched versus mismatched DLI, dosing progressed from 1 x 10 7 to 5 x10 7 CD3 + cells/kg for matched HCT recipients and 1 x 10 6 to 5 x10 7 CD3 + cells/kg for mismatched HCT recipients. The primary endpoint was the feasibility of the product, defined as: successful harvest; expansion; infusion; and absence of grade III-IV acute GVHD for 90 days after infusion. Evaluation for HLA loss was performed using next-generation sequencing (NGS) of HLA loci. Single cell RNA sequencing (scRNAseq) was performed using 10x Genomics technology, Cell Ranger (v6.0.2), and Seurat (v4.3.0). Results: Thirty-two patients enrolled and underwent bone marrow harvest. Two patients were inaspirable, and five patients were not expanded due to low cell counts or high tumor volume. Twenty-five patients underwent alloMILs expansion, of which five failed to expand. Twenty patients received an infusion and six received a second infusion. Of the twenty patients receiving an infusion, eleven had multiple myeloma (MM) or plasma cell leukemia; five had acute myeloid leukemia (AML); one had chronic myeloid leukemia in blast phase; and three had chronic lymphocytic leukemia. Harvest, expansion, and infusion were well tolerated with minimal adverse events. No patient developed acute or chronic GVHD after infusion. Dosing advanced to the highest level of 5 x10 7 CD3 + cells/kg in both the mismatched and matched HCT cohorts. At day 60, the overall response rate (composite of complete (CR) and partial responses) was 40 percent (8/20) in the entire cohort; 33.3 percent (2/6) in the myeloid cohort (Figure 1A); 30 percent (3/10 evaluable patients) in the MM cohort; and 66.7 percent (2/3) in the lymphoid cohort. One MM patient achieved sustained &amp;gt; 2 years long CR and one AML patient is in ongoing CR, both of whom received alloMILs with no or minimal disease followed by maintenance therapy. HLA loss analysis was performed on pre-expansion samples from three AML patients receiving a mismatched HCT and alloMILs. Both non-responders in this cohort had loss of the host mismatched HLA haplotype. ScRNAseq on pre-expansion samples from ten AML patients, eleven MM patients, and four AML patients in remission after HCT (control) yielded 197 522 total cells and 32 587 CD8 + T cells. CD8 + T cells expressing a senescent-like gene signature previously associated with dysfunctional tumor killing in AML patients (Mazziotta et al ASH 2022) were associated with non-response primarily in AML patients (observed log2 fold change -2.48 compared with responders; FDR = 0.0017) (Figure 1B). Conclusions: AlloMILs demonstrated feasibility, a favorable safety profile, and early evidence of clinical activity in patients with measurable residual disease. The maximum dose of 5 x10 7 CD3 + cells/kg was reached, and no patient developed acute or chronic GVHD. HLA loss and CD8 + T cell senescence may be associated with non-response in AML patients. A clinical trial utilizing alloMILs as maintenance therapy for patients with measurable residual disease is in development.